Electronic Supplementary Material (ESI) for Environmental Science: Water Research & Technology

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Electronic Supplementary Material (ESI) for Environmental Science: Water Research & Technology. This journal is © The Royal Society of Chemistry 2016

Supplementary material

Eukaryotic community diversity and spatial variation during drinking water production (by seawater desalination) and distribution in a full-scale network

A. Belila, J. El-Chakhtoura, P.E. Saikaly, M.C.M. van Loosdrecht, J.S. Vrouwenvelder

Figure S1. KAUST campus map showing the drinking water sampling locations for (i) the produced water (PW) feeding the distribution network and (ii) five household tap water sampling locations (TP1-TP5) in the distribution network.

Figure S2. Venn diagrams displaying the overlap between the eukaryotic communities across different sampling locations based on OTUs (3% distance cutoff): (A) during the pretreatment step and (B) during the reverse osmosis and post-treatment steps. IW: inlet water; SMFP: spruce media filter permeate; RO1: seawater reverse osmosis permeate; RO2: brackish water reverse osmosis permeate; PW: produced water.

Figure S3. Venn diagram displaying the overlap between the eukaryotic communities of the produced water (PW) and the combined tap water samples (TP1-TP5) based on OTUs (3% cutoff).

100%# Unclassified#Fungi# Unclassified#Chytridiomycota# Unclassified#Basidiomycota#

80%# Tremellomycetes# Schizosaccharomycetes# Saccharomycetes#

Pezizomycetes# 60%# NeocallimasJgomycetes#

! LeoJomycetes# Glomeromycetes# 40%# Exobasidiomycetes# Relativeabundance EuroJomycetes# Dothideomycetes# 20%# Chytridiomycetes# Blastocladiomycetes# AgaricosJlbomycetes#

0%# Agaricomycetes# IW# SMFP# RO1# RO2# PW# TP1# TP2# TP3# TP4# TP5# Figure S4. Variation of the fungal community composition (class level) during water desalination and after distribution. IW: inlet water; SMFP: spruce media filter permeate; RO1: seawater reverse osmosis permeate; RO2: brackish water reverse osmosis permeate; PW: produced water; TP1-TP5: tap water samples.

Table S1. Number of shared OTUs between tap water samples

TP1 TP2 TP3 TP4 TP5

TP1 - 11 (8%) 0 4 (3.2%) 0

TP2 - - 61 (27%) 2 (0.9%) 1 (0.4%)

TP3 - - - 51 (23.4%) 8 (3.1%)

TP4 - - - - 7 (3.4%)

TP5 - - - - - TP1-TP5: Tap water samples. Values in parentheses represent the percentage of the shared OTUs compared to the total number of OTUs in both locations.

Table S2. Classification of OTUs recovered from tap water samples.

Percentage homology OTU percentage Tap location ID Species (%) (%) TP1 Nematoda Plectus minimus 97.3-98.0 22.82 Fungi Cochliobolus sp. 007(l)1 97.1-99.0 22.15 Chordata Budorcasta xicolor 97.3 10.07 Fungi Pleospora herbarum 97.1-98.3 9.40 Fungi Cryptococcus ater 97.2-98.4 8.72 Fungi Uncultured fungus 97.1-99.4 8.05 Fungi Uncultured ascomycota 98.2-98.4 6.71 Nematoda Diplolaimelloides myeli 98.3 6.71 Chordata Blarina sp. 98.4-98.9 5.37 TP2 Fungi Cladosporium cladosporioides 98.3-97.1 34.21 Arthropoda Blattella germanica 97.3-99.2 23.68 Fungi Uncultured cladosporium 97.5-98.0 15.79 Fungi Uncultured fungus 97.6-98.5 9.21 Fungi Aureobasidium pullulan 97.1-97.7 6.58 Fungi Cladosporium sp. zhi2 97.1-97.2 5.26 Fungi Dothidea ribesia 97.5-97.6 2.63 Fungi Herpotrichiellaceae sp. lm294 98.0 1.32 Fungi Hymenoscyphus scutula 97.5 1.32 TP3 Fungi Pleospora herbarum 97.1-99.1 23.78 Fungi Dothidea insculpta 97.3-98.8 20.28 Fungi Cochliobolus sp. 007(l)1_1 97.1-98.3 13.99 Fungi Aureobasidium pullulans 97.1-98.7 13.29 Fungi Cladosporium cladosporioides 98.3 8.39 Fungi Uncultured ascomycota 98.2-98.4 6.99 Nematoda Plectus minimus 97.0 6.99 Fungi Uncultured fungus 97.3-98.5 3.50 Phaeophyceae Undaria pinnatifida 98.2 2.80 TP4 Fungi Uncultured fungus 97.2-99.0 36.84 Fungi Acephala sp. 1 97.4-98.7 16.84 Fungi Cladosporium sp. zhi2 97.1-97.3 9.47 Chordata Blarina sp. 97.3-98.4 8.42 Fungi Cladosporium cladosporioides 98.1 7.37 Fungi Uncultured fungus 97.6-98.5 6.32 Arthropoda Gymnoglyphus osu 97.5 5.26 Chordata Bubalus bubalis 97.1-97.5 5.26 Fungi Cryptococcus ater 97.1-97.3 4.21 TP5 Porifera Grantessa sp. 97.1-98.4 28.57 Chordata Bubalus bubalis 97.1-97.3 21.43 Arthropoda Blattella germanica 97.3-99.2 12.86 Chordata Budorcasta xicolor 97.4 10.71

Chordata Blarina sp 97.4-99.1 7.86 Fungi Uncultured fungus 97.9 7.14 Fungi Cochliobolus sp. 007(l)1_1 97.7 6.43 Fungi Uncultured fungus 97.6-98.5 5.00