DOCSLIB.ORG

Genomics-Driven Discovery, Characterization, and Engineering of Fungal Secondary Metabolites

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Genomics-Driven Discovery, Characterization, and Engineering of Fungal Secondary Metabolites Downloaded from orbit.dtu.dk on: Oct 09, 2021 Genomics-driven discovery, characterization, and engineering of fungal secondary metabolites Wolff, Peter Betz Publication date: 2020 Document Version Publisher's PDF, also known as Version of record Link back to DTU Orbit Citation (APA): Wolff, P. B. (2020). Genomics-driven discovery, characterization, and engineering of fungal secondary metabolites. Technical University of Denmark. General rights Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights. Users may download and print one copy of any publication from the public portal for the purpose of private study or research. You may not further distribute the material or use it for any profit-making activity or commercial gain You may freely distribute the URL identifying the publication in the public portal If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim. GENOMICS-DRIVEN DISCOVERY, CHARACTERIZATION, AND ENGINEERING OF FUNGAL SECONDARY METABOLITES Peter Betz Wolff A thesis submitted for the degree of Doctor of Philosophy February 2020 Genomics-driven discovery, characterization, and engineering of fungal secondary metabolites Peter Betz Wolff, 27th of February 2020 Address: Technical University of Denmark Department of Biotechnology and Biomedicine Sølftofts Plads, Building 223 2800 Kgs. Lyngby Denmark Supervisors: Professor Uffe Hasbro Mortensen Professor Thomas Ostenfeld Larsen Preface Preface This Ph.D. thesis describes the results of my study initiated at the Technical University of Denmark (DTU) in September 2016 and finished in February 2020. The study was conducted at DTU Bioengineering, department of Biotechnology and Biomedicine under the supervi- sion of professor Uffe Hasbro Mortensen and Professor Thomas Ostenfeld Larsen. The Novo Nordisk foundation supported the work, grand NNF15OC0016610. During my Ph.D. project, I have had the opportunity to learn new techniques for molecular engineering within fungal secondary metabolism, gain a broader knowledge for project management, and participate in inspiring conferences worldwide. Kgs. Lyngby, February 2020 Peter Betz Wolff Page i Contents Contents Preface i Acknowledgements v Summary vi Sammenfatning ix Publications xii Conference contributions xiii Abbreviations xiv 1 Project motivation and objective 1 Project motivation . .1 Project objective . .1 References . .3 2 Background 4 Biology and chemistry . .4 The fifth kingdom . .4 From genes to natural products . .5 The biosynthesis of natural products . .8 Pathways . 14 Tetracyclines . 18 Mode of action . 19 Structure activity relationship . 20 Bacteria resistance mechanisms . 22 Tools......................................... 23 Bioinformatics . 23 Quantitative real-time PCR . 23 Chemical analysis and dereplication . 24 Molecular networking for biosynthetic pathway elucidation . 24 References . 26 3 Fungal Synthetic Biology Tools to Facilitate Utilization of Secondary Metabo- lites: Discovery; Heterologous Production; and Biosynthesis of Biocombinato- rial Compounds 38 Abstract . 38 Introduction . 38 Page ii Contents Discovery of secondary metabolite pathways in natural hosts . 39 CRISPR Cas9 in filamentous fungi . 40 Gene-expression cassettes . 41 Activation of silent BGCs in the native host . 43 Global regulation on and beyond chromatin level . 44 Linking fungal biosynthetic gene clusters to secondary metabolite products 46 Discovery and characterization using heterologous expression . 47 Fungal cell factories . 49 Optimization of fungal cell factories . 49 Expanding the chemical space via biocombinatorial synthetic biology . 51 Synthetic Pathways for Production of Natural and Non-natural Products . 51 Synthetic Enzymes for Production of Non-Native Products . 54 Perspective . 56 References . 57 4 Acurin A; a novel hybrid compound; biosynthesized by individually translated PKS- and NRPS-encoding genes in Aspergillus aculeatus 69 Abstract . 69 Introduction . 70 Materials and Methods . 71 Results and Discussion . 76 Acurin A – a novel PK-NRP hybrid compound from Aspergillus aculeatus . 76 Identification of putative PKS and NRPS genes for the production of acurin 78 Separate PKS- and NRPS enzymes synthesize the acurin A core structure . 80 Uncoupled PKS and NRPS activity is conserved in a clade in section Nigri . 81 The acurin BGC contains genes encoding tailoring enzymes and a TF . 85 Gene deletions identify genes in the acr biosynthetic gene cluster . 87 Proposed biosynthesis of acurin A . 89 Conclusion . 91 Acknowledgements . 92 References . 92 5 Genetic Manipulation and Molecular Networking for Biosynthetic Pathway Characterization of Tetracycline-like Compounds in Aspergillus sydowii 97 Abstract . 97 Introduction . 97 Results . 98 Screening the genus Aspergillus for a potential tetracycline-likeBGC . 98 Homologous expression of a putative pathway-specific transcription factor . 100 Targeted gene deletions for tetracycline-like BGC characterization . 102 Molecular networking as a tool for intermediate characterization . 103 Page iii Contents Proposed biosynthetic pathway . 106 Discussion . 109 Screening the genus Aspergillus for potential tetracycline BGCs . 109 Overexpression of nstR leads to an unnatural behavior in A. sydowii .... 109 Targeted gene deletions verify neosartoricinB as the main BGC product . 110 Molecular networking as a tool for biosynthetic pathway characterization . 110 Enzyme order in the pathway is defined by the environmental factors . 110 Conclusion . 111 Acknowledgements . 112 Experimental . 112 References . 115 6 Expression Platform for Biocombinatorial Decoration of a Fungal-derived Tetra- cycline in Aspergillus niger 118 Abstract . 118 Introduction . 118 Results . 119 Homologous reconstitution of the ada pathway in A. niger ......... 119 Verification and analysis of tetracycline production in the platform strains . 122 AMA1-based expression of two tailoring-enzymes for further validation . 122 Identifying tailoring enzyme-encoding genes for further expression . 124 Discussion . 126 Conclusion . 127 Acknowledgements . 127 Experimental section . 127 References . 129 7 Overall discussion, conclusion, and perspectives 131 References . 131 Appendix I (Chapter 4) 137 Appendix II (Chapter 5) 183 Appendix III (Chapter 6) 214 Page iv Acknowledgements Acknowledgements Firstly, I would like to express my gratitude to my supervisors Prof. Uffe Hasbro Mortensen and Prof. Thomas Ostenfeld Larsen, for the opportunity to be part of this research project. These last three and a half years have been an exciting, rewarding, and educational experience for me, and it would not have been possible without the invaluable guidance I received from both throughout this research. They have taught me the methodology needed to carry out my research and to present the results in the most precise way possible. Their motivation and vision for the project have deeply inspired me, and I feel incredibly grateful to have had the chance to work with them. My former master supervisor Assoc. Prof. Jakob Blæsbjerg Hoof has given me valuable support and been a mentor for me throughout this project. I thank you for all the enjoyable scientific discussion we have had over the years, they have inspired me to do more. I want to thank Karolina Subko for the fantastic cooperation on this project. You are a brilliant chemist with innovative ideas, an eye for details, and a great drive. I have enjoyed working together with you, and I have gained a lot more insights into the world of chemistry through our many talks. Thank you. Working at the Technical University of Denmark (DTU) and especially in the Aspergillus laboratory at the section for synthetic biology, has been one of the best working experiences in my career. My co-workers and fellow Ph.D. candidates have always been helpful, and with a great sense of humor. I have particularly appreciated my productive discussions with Kresten Jon Korup Kromphardt and Jakob Kræmmer Haar Rendsvig, they have both contributed to improving my understanding of fungal science. Also, Zofia Dorota Jarczynska has been a great help in the laboratory with guidance and advice. And a special thanks to Andreas Møllerhøj Vestergaard for keeping us all entertained in his inexhaustible search for fungal monooxygenases. Finally, to my caring, loving, and supportive wife, Naja: you have my deepest gratitude. You have been a guardian angel throughout these years, and the project would never have been finished if it wasn’t for you. Your loving care for our toddler daughter and baby son, in my absence, while writing, has been admirable and an inspiration to me. You have helped me in my darkest hour of frustration, listened, and always reassured me that I could do this. For that, I owe you everything. Page v Summary Summary Filamentous fungi can produce many natural products (NPs) with both beneficial properties (e.g., bioactive pharmaceutical compounds), as well as harmful substances (e.g., mycotoxins). For the past decade, microbial whole-genome sequencing projects have identified a sizeable genetic potential for discovering yet more unknown natural products, which include complex derivatives of already-approved drugs. Filamentous fungi, here among the genus Aspergillus, has been a source
Load more

Recommended publications
  • Baudoinia, a New Genus to Accommodate Torula Compniacensis
    Mycologia, 99(4), 2007, pp. 592–601. # 2007 by The Mycological Society of America, Lawrence, KS 66044-8897 Baudoinia, a new genus to accommodate Torula compniacensis James A. Scott1 sooty, fungal growth, so-called ‘‘warehouse staining’’, Department of Public Health Sciences, University of has been well known anecdotally in the spirits Toronto, Toronto, Ontario, Canada M5T 1R4, and industry for many years. During our investigation we Sporometrics Inc., 219 Dufferin Street, Suite 20C, reviewed a number of internal, industry-commis- Toronto, Ontario, M6K 1Y9 Canada sioned studies of this phenomenon from Asia, Europe Wendy A. Untereiner and North America that attempted to ascertain the Zoology Department, Brandon University, Brandon, taxonomic composition of this material using culture- Manitoba, R7A 6A9 Canada based techniques. Despite the distinctiveness of this Juliet O. Ewaze habitat and the characteristic sooty appearance of the Bess Wong growth, these reports persistently implicated the same Department of Public Health Sciences, University of etiologically implausible set of ubiquitous environ- Toronto, Toronto, Ontario, Canada M5T 1R4, and mental microfungi, chiefly Aureobasidium pullulans Sporometrics Inc., 219 Dufferin Street, Suite 20C, (de Bary) Arnaud, Epicoccum nigrum Link, and Toronto, Ontario, M6K 1Y9 Canada species of Alternaria Nees, Aspergillus P. Micheli ex. David Doyle Haller, Cladosporium Link, and Ulocladium Preuss. A Hiram Walker & Sons Ltd./Pernod Ricard North search of the post-1950s scientific literature indexed America, Windsor, Ontario, N8Y 4S5 Canada by ISI Web of Knowledge failed to yield references to this phenomenon. However a broader search of trade literature and the Web led us to the name Torula Abstract: Baudoinia gen.
    [Show full text]
  • Variable Absorption of Mutational Trends by Prion-Forming Domains During Saccharomycetes Evolution
    Variable absorption of mutational trends by prion-forming domains during Saccharomycetes evolution Paul M. Harrison Department of Biology, McGill University, Monteal, Quebec, Canada ABSTRACT Prions are self-propagating alternative states of protein domains. They are linked to both diseases and functional protein roles in eukaryotes. Prion-forming domains in Saccharomyces cerevisiae are typically domains with high intrinsic protein disorder (i.e., that remain unfolded in the cell during at least some part of their functioning), that are converted to self-replicating amyloid forms. S. cerevisiae is a member of the fungal class Saccharomycetes, during the evolution of which a large population of prion-like domains has appeared. It is still unclear what principles might govern the molecular evolution of prion-forming domains, and intrinsically disordered domains generally. Here, it is discovered that in a set of such prion-forming domains some evolve in the fungal class Saccharomycetes in such a way as to absorb general mutation biases across millions of years, whereas others do not, indicating a spectrum of selection pressures on composition and sequence. Thus, if the bias-absorbing prion formers are conserving a prion-forming capability, then this capability is not interfered with by the absorption of bias changes over the duration of evolutionary epochs. Evidence is discovered for selective constraint against the occurrence of lysine residues (which likely disrupt prion formation) in S. cerevisiae prion-forming domains as they evolve across Saccharomycetes. These results provide a case study of the absorption of mutational trends by compositionally biased domains, and suggest methodology for assessing selection pressures on the composition of intrinsically disordered regions.
    [Show full text]
  • Microsporum Canis Genesig Standard
    Primerdesign TM Ltd Microsporum canis PQ-loop repeat protein gene genesig® Standard Kit 150 tests For general laboratory and research use only Quantification of Microsporum canis genomes. 1 genesig Standard kit handbook HB10.04.10 Published Date: 09/11/2018 Introduction to Microsporum canis Microsporum canis is a zoophilic dermatophyte which is responsible for dermatophytosis in dogs and cats. They cause superficial infections of the scalp (tinea capitis) in humans and ringworm in cats and dogs. They belong to the family Arthrodermataceae and are most commonly found in humid and warm climates. They have numerous multi-celled macroconidia which are typically spindle-shaped with 5-15 cells, verrucose, thick-walled, often having a terminal knob and 35-110 by 12-25 µm. In addition, they produce septate hyphae and microconidia and the Microsporum canis genome is estimated at 23 Mb. The fungus is transmitted from animals to humans when handling infected animals or by contact with arthrospores contaminating the environment. Spores are very resistant and can live up to two years infecting animals and humans. They will attach to the skin and germinate producing hyphae, which will then grow in the dead, superficial layers of the skin, hair or nails. They secrete a 31.5 kDa keratinolytic subtilisin-like protease as well as three other subtilisin- like proteases (SUBs), SUB1, SUB2 and SUB3, which cause damage to the skin and hair follicle. Keratinolytic protease also provides the fungus nutrients by degrading keratin structures into easily absorbable metabolites. Infection leads to a hypersensitive reaction of the skin. The skin becomes inflamed causing the fungus to move away from the site to normal, uninfected skin.
    [Show full text]
  • Genome Diversity and Evolution in the Budding Yeasts (Saccharomycotina)
    | YEASTBOOK GENOME ORGANIZATION AND INTEGRITY Genome Diversity and Evolution in the Budding Yeasts (Saccharomycotina) Bernard A. Dujon*,†,1 and Edward J. Louis‡,§ *Department Genomes and Genetics, Institut Pasteur, Centre National de la Recherche Scientifique UMR3525, 75724-CEDEX15 Paris, France, †University Pierre and Marie Curie UFR927, 75005 Paris, France, ‡Centre for Genetic Architecture of Complex Traits, and xDepartment of Genetics, University of Leicester, LE1 7RH, United Kingdom ORCID ID: 0000-0003-1157-3608 (E.J.L.) ABSTRACT Considerable progress in our understanding of yeast genomes and their evolution has been made over the last decade with the sequencing, analysis, and comparisons of numerous species, strains, or isolates of diverse origins. The role played by yeasts in natural environments as well as in artificial manufactures, combined with the importance of some species as model experimental systems sustained this effort. At the same time, their enormous evolutionary diversity (there are yeast species in every subphylum of Dikarya) sparked curiosity but necessitated further efforts to obtain appropriate reference genomes. Today, yeast genomes have been very informative about basic mechanisms of evolution, speciation, hybridization, domestication, as well as about the molecular machineries underlying them. They are also irreplaceable to investigate in detail the complex relationship between genotypes and phenotypes with both theoretical and practical implications. This review examines these questions at two distinct levels offered by the broad evolutionary range of yeasts: inside the best-studied Saccharomyces species complex, and across the entire and diversified subphylum of Saccharomycotina. While obviously revealing evolutionary histories at different scales, data converge to a remarkably coherent picture in which one can estimate the relative importance of intrinsic genome dynamics, including gene birth and loss, vs.
    [Show full text]
  • Diversification of Fungal Chitinases and Their Functional Differentiation in 2 Histoplasma Capsulatum 3
    bioRxiv preprint doi: https://doi.org/10.1101/2020.06.09.137125; this version posted June 16, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-ND 4.0 International license. 1 Diversification of fungal chitinases and their functional differentiation in 2 Histoplasma capsulatum 3 4 Kristie D. Goughenour1*, Janice Whalin1, 5 Jason C. Slot2, Chad A. Rappleye1# 6 7 1 Department of Microbiology, Ohio State University 8 2 Department of Plant Pathology, Ohio State University 9 10 11 #corresponding author: 12 [email protected] 13 614-247-2718 14 15 *current affiliation: 16 Division of Pulmonary and Critical Care Medicine 17 University of Michigan 18 VA Ann Arbor Healthcare System, Research Service 19 Ann Arbor, Michigan, USA 20 21 22 running title: Fungal chitinases 23 24 keywords: chitinase, GH18, fungi, Histoplasma 25 bioRxiv preprint doi: https://doi.org/10.1101/2020.06.09.137125; this version posted June 16, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-ND 4.0 International license. 26 ABSTRACT 27 Chitinases enzymatically hydrolyze chitin, a highly abundant biomolecule with many potential 28 industrial and medical uses in addition to their natural biological roles. Fungi are a rich source of 29 chitinases, however the phylogenetic and functional diversity of fungal chitinases are not well 30 understood.
    [Show full text]
  • Distribution of Methionine Sulfoxide Reductases in Fungi and Conservation of the Free- 2 Methionine-R-Sulfoxide Reductase in Multicellular Eukaryotes
    bioRxiv preprint doi: https://doi.org/10.1101/2021.02.26.433065; this version posted February 27, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 1 Distribution of methionine sulfoxide reductases in fungi and conservation of the free- 2 methionine-R-sulfoxide reductase in multicellular eukaryotes 3 4 Hayat Hage1, Marie-Noëlle Rosso1, Lionel Tarrago1,* 5 6 From: 1Biodiversité et Biotechnologie Fongiques, UMR1163, INRAE, Aix Marseille Université, 7 Marseille, France. 8 *Correspondence: Lionel Tarrago ([email protected]) 9 10 Running title: Methionine sulfoxide reductases in fungi 11 12 Keywords: fungi, genome, horizontal gene transfer, methionine sulfoxide, methionine sulfoxide 13 reductase, protein oxidation, thiol oxidoreductase. 14 15 Highlights: 16 • Free and protein-bound methionine can be oxidized into methionine sulfoxide (MetO). 17 • Methionine sulfoxide reductases (Msr) reduce MetO in most organisms. 18 • Sequence characterization and phylogenomics revealed strong conservation of Msr in fungi. 19 • fRMsr is widely conserved in unicellular and multicellular fungi. 20 • Some msr genes were acquired from bacteria via horizontal gene transfers. 21 1 bioRxiv preprint doi: https://doi.org/10.1101/2021.02.26.433065; this version posted February 27, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license.
    [Show full text]
  • Fungal Evolution: Major Ecological Adaptations and Evolutionary Transitions
    Biol. Rev. (2019), pp. 000–000. 1 doi: 10.1111/brv.12510 Fungal evolution: major ecological adaptations and evolutionary transitions Miguel A. Naranjo-Ortiz1 and Toni Gabaldon´ 1,2,3∗ 1Department of Genomics and Bioinformatics, Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, Dr. Aiguader 88, Barcelona 08003, Spain 2 Department of Experimental and Health Sciences, Universitat Pompeu Fabra (UPF), 08003 Barcelona, Spain 3ICREA, Pg. Lluís Companys 23, 08010 Barcelona, Spain ABSTRACT Fungi are a highly diverse group of heterotrophic eukaryotes characterized by the absence of phagotrophy and the presence of a chitinous cell wall. While unicellular fungi are far from rare, part of the evolutionary success of the group resides in their ability to grow indefinitely as a cylindrical multinucleated cell (hypha). Armed with these morphological traits and with an extremely high metabolical diversity, fungi have conquered numerous ecological niches and have shaped a whole world of interactions with other living organisms. Herein we survey the main evolutionary and ecological processes that have guided fungal diversity. We will first review the ecology and evolution of the zoosporic lineages and the process of terrestrialization, as one of the major evolutionary transitions in this kingdom. Several plausible scenarios have been proposed for fungal terrestralization and we here propose a new scenario, which considers icy environments as a transitory niche between water and emerged land. We then focus on exploring the main ecological relationships of Fungi with other organisms (other fungi, protozoans, animals and plants), as well as the origin of adaptations to certain specialized ecological niches within the group (lichens, black fungi and yeasts).
    [Show full text]
  • Based on a Newly-Discovered Species
    A peer-reviewed open-access journal MycoKeys 76: 1–16 (2020) doi: 10.3897/mycokeys.76.58628 RESEARCH ARTICLE https://mycokeys.pensoft.net Launched to accelerate biodiversity research The insights into the evolutionary history of Translucidithyrium: based on a newly-discovered species Xinhao Li1, Hai-Xia Wu1, Jinchen Li1, Hang Chen1, Wei Wang1 1 International Fungal Research and Development Centre, The Research Institute of Resource Insects, Chinese Academy of Forestry, Kunming 650224, China Corresponding author: Hai-Xia Wu ([email protected], [email protected]) Academic editor: N. Wijayawardene | Received 15 September 2020 | Accepted 25 November 2020 | Published 17 December 2020 Citation: Li X, Wu H-X, Li J, Chen H, Wang W (2020) The insights into the evolutionary history of Translucidithyrium: based on a newly-discovered species. MycoKeys 76: 1–16. https://doi.org/10.3897/mycokeys.76.58628 Abstract During the field studies, aTranslucidithyrium -like taxon was collected in Xishuangbanna of Yunnan Province, during an investigation into the diversity of microfungi in the southwest of China. Morpho- logical observations and phylogenetic analysis of combined LSU and ITS sequences revealed that the new taxon is a member of the genus Translucidithyrium and it is distinct from other species. Therefore, Translucidithyrium chinense sp. nov. is introduced here. The Maximum Clade Credibility (MCC) tree from LSU rDNA of Translucidithyrium and related species indicated the divergence time of existing and new species of Translucidithyrium was crown age at 16 (4–33) Mya. Combining the estimated diver- gence time, paleoecology and plate tectonic movements with the corresponding geological time scale, we proposed a hypothesis that the speciation (estimated divergence time) of T.
    [Show full text]
  • Bodenmikrobiologie (Version: 07/2019)
    Langzeitmonitoring von Ökosystemprozessen - Methoden-Handbuch Modul 04: Bodenmikrobiologie (Version: 07/2019) www.hohetauern.at Impressum Impressum Für den Inhalt verantwortlich: Dr. Fernando Fernández Mendoza & Prof. Mag Dr. Martin Grube Institut für Biologie, Bereich Pflanzenwissenschaften, Universität Graz, Holteigasse 6, 8010 Graz Nationalparkrat Hohe Tauern, Kirchplatz 2, 9971 Matrei i.O. Titelbild: Ein Transekt im Untersuchungsgebiet Innergschlöss (2350 m üNN) wird im Jahr 2017 beprobt. © Newesely Zitiervorschlag: Fernández Mendoza F, Grube M (2019) Langzeitmonitoring von Ökosystemprozessen im Nationalpark Hohe Tauern. Modul 04: Mikrobiologie. Methoden-Handbuch. Verlag der Österreichischen Akademie der Wissenschaften, Wien. ISBN-Online: 978-3-7001-8752-3, doi: 10.1553/GCP_LZM_NPHT_Modul04 Weblink: https://verlag.oeaw.ac.at und http://www.parcs.at/npht/mmd_fullentry.php?docu_id=38612 Inhaltsverzeichnis Zielsetzung ...................................................................................................................................................... 1 Inhalt Vorbereitungsarbeit und benötigtes Material ................................................................................................... 2 a. Materialien für die Probenahme und Probenaufbewahrung ................................................................ 2 b. Materialien und Geräte für die Laboranalyse ...................................................................................... 2 Arbeitsablauf ...................................................................................................................................................
    [Show full text]
  • Fungal Planet Description Sheets: 716–784 By: P.W
    Fungal Planet description sheets: 716–784 By: P.W. Crous, M.J. Wingfield, T.I. Burgess, G.E.St.J. Hardy, J. Gené, J. Guarro, I.G. Baseia, D. García, L.F.P. Gusmão, C.M. Souza-Motta, R. Thangavel, S. Adamčík, A. Barili, C.W. Barnes, J.D.P. Bezerra, J.J. Bordallo, J.F. Cano-Lira, R.J.V. de Oliveira, E. Ercole, V. Hubka, I. Iturrieta-González, A. Kubátová, M.P. Martín, P.-A. Moreau, A. Morte, M.E. Ordoñez, A. Rodríguez, A.M. Stchigel, A. Vizzini, J. Abdollahzadeh, V.P. Abreu, K. Adamčíková, G.M.R. Albuquerque, A.V. Alexandrova, E. Álvarez Duarte, C. Armstrong-Cho, S. Banniza, R.N. Barbosa, J.-M. Bellanger, J.L. Bezerra, T.S. Cabral, M. Caboň, E. Caicedo, T. Cantillo, A.J. Carnegie, L.T. Carmo, R.F. Castañeda-Ruiz, C.R. Clement, A. Čmoková, L.B. Conceição, R.H.S.F. Cruz, U. Damm, B.D.B. da Silva, G.A. da Silva, R.M.F. da Silva, A.L.C.M. de A. Santiago, L.F. de Oliveira, C.A.F. de Souza, F. Déniel, B. Dima, G. Dong, J. Edwards, C.R. Félix, J. Fournier, T.B. Gibertoni, K. Hosaka, T. Iturriaga, M. Jadan, J.-L. Jany, Ž. Jurjević, M. Kolařík, I. Kušan, M.F. Landell, T.R. Leite Cordeiro, D.X. Lima, M. Loizides, S. Luo, A.R. Machado, H. Madrid, O.M.C. Magalhães, P. Marinho, N. Matočec, A. Mešić, A.N. Miller, O.V. Morozova, R.P. Neves, K. Nonaka, A. Nováková, N.H.
    [Show full text]
  • Molecular Identification of Fungi
    Molecular Identification of Fungi Youssuf Gherbawy l Kerstin Voigt Editors Molecular Identification of Fungi Editors Prof. Dr. Youssuf Gherbawy Dr. Kerstin Voigt South Valley University University of Jena Faculty of Science School of Biology and Pharmacy Department of Botany Institute of Microbiology 83523 Qena, Egypt Neugasse 25 [email protected] 07743 Jena, Germany [email protected] ISBN 978-3-642-05041-1 e-ISBN 978-3-642-05042-8 DOI 10.1007/978-3-642-05042-8 Springer Heidelberg Dordrecht London New York Library of Congress Control Number: 2009938949 # Springer-Verlag Berlin Heidelberg 2010 This work is subject to copyright. All rights are reserved, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilm or in any other way, and storage in data banks. Duplication of this publication or parts thereof is permitted only under the provisions of the German Copyright Law of September 9, 1965, in its current version, and permission for use must always be obtained from Springer. Violations are liable to prosecution under the German Copyright Law. The use of general descriptive names, registered names, trademarks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. Cover design: WMXDesign GmbH, Heidelberg, Germany, kindly supported by ‘leopardy.com’ Printed on acid-free paper Springer is part of Springer Science+Business Media (www.springer.com) Dedicated to Prof. Lajos Ferenczy (1930–2004) microbiologist, mycologist and member of the Hungarian Academy of Sciences, one of the most outstanding Hungarian biologists of the twentieth century Preface Fungi comprise a vast variety of microorganisms and are numerically among the most abundant eukaryotes on Earth’s biosphere.
    [Show full text]
  • IV Workshop Libro De Abstracts
    IV Workshop Libro de Abstracts Red nacional sobre las micotoxinas y hongos toxigénicos y de sus procesos de descontaminación 29-31 de mayo de 2019 -Facultad de Farmacia y Nutrición - Universidad de Navarra http://micofood.es ISSN: 2444-3158 Editado en Valencia por: Red Nacional sobre las micotoxinas y hongos toxigénicos y de sus procesos de descontaminación. Raquel Torrijos, Juan Manuel Quiles. Micofood 2019 CONTENIDO COMITÉ CIENTÍFICO 2 PROYECTO MICOFOOD 4 BIENVENIDA 5 PROGRAMA 6 ÍNDICE DE ABSTRACTS 9 COMUNICACIONES ORALES 12 POSTERS 32 COLABORADORES 57 NOTAS 58 1 COMITÉ CIENTÍFICO: Jordi Mañes Vinuesa (Universitat de València) Mónica Fernández Franzón (Universitat de València) Giuseppe Meca (Universitat de València) Misericordia Jiménez Escamilla (Universitat de València) Mar Rodríguez Jovita (Universidad de Extremadura) Vicente Sanchis Almenar (Universitat de Lleida) Agustín Ariño Moneva (Universidad de Zaragoza) Covadonga Vázquez Estévez (Universidad Complutense de Madrid) Elena González-Peñas (Universidad de Navarra) Adela López de Cerain (Universidad de Navarra) Luis González Candelas (Consejo Superior de Investigaciones Científicas) Alberto Cepeda Sáez (Universidad de Santiago de Compostela) Francisco Javier Cabañes Saenz (Universitat Autònoma de Barcelona) Ana María García Campaña (Universidad de Granada) COMITÉ ORGANIZADOR: Jordi Mañes Vinuesa (Universitat de València) Elena González-Peñas (Universidad de Navarra) Adela López de Cerain (Universidad de Navarra) Monica Fernandez Franzon (Universitat de València) Cristina Juan Garcia
    [Show full text]