Analysis and Optimisation of Plant Biomass Degrading Enzyme Production in Aspergillus
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Distribution of Methionine Sulfoxide Reductases in Fungi and Conservation of the Free- 2 Methionine-R-Sulfoxide Reductase in Multicellular Eukaryotes
bioRxiv preprint doi: https://doi.org/10.1101/2021.02.26.433065; this version posted February 27, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 1 Distribution of methionine sulfoxide reductases in fungi and conservation of the free- 2 methionine-R-sulfoxide reductase in multicellular eukaryotes 3 4 Hayat Hage1, Marie-Noëlle Rosso1, Lionel Tarrago1,* 5 6 From: 1Biodiversité et Biotechnologie Fongiques, UMR1163, INRAE, Aix Marseille Université, 7 Marseille, France. 8 *Correspondence: Lionel Tarrago ([email protected]) 9 10 Running title: Methionine sulfoxide reductases in fungi 11 12 Keywords: fungi, genome, horizontal gene transfer, methionine sulfoxide, methionine sulfoxide 13 reductase, protein oxidation, thiol oxidoreductase. 14 15 Highlights: 16 • Free and protein-bound methionine can be oxidized into methionine sulfoxide (MetO). 17 • Methionine sulfoxide reductases (Msr) reduce MetO in most organisms. 18 • Sequence characterization and phylogenomics revealed strong conservation of Msr in fungi. 19 • fRMsr is widely conserved in unicellular and multicellular fungi. 20 • Some msr genes were acquired from bacteria via horizontal gene transfers. 21 1 bioRxiv preprint doi: https://doi.org/10.1101/2021.02.26.433065; this version posted February 27, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. -
Fungal Planet Description Sheets: 716–784 By: P.W
Fungal Planet description sheets: 716–784 By: P.W. Crous, M.J. Wingfield, T.I. Burgess, G.E.St.J. Hardy, J. Gené, J. Guarro, I.G. Baseia, D. García, L.F.P. Gusmão, C.M. Souza-Motta, R. Thangavel, S. Adamčík, A. Barili, C.W. Barnes, J.D.P. Bezerra, J.J. Bordallo, J.F. Cano-Lira, R.J.V. de Oliveira, E. Ercole, V. Hubka, I. Iturrieta-González, A. Kubátová, M.P. Martín, P.-A. Moreau, A. Morte, M.E. Ordoñez, A. Rodríguez, A.M. Stchigel, A. Vizzini, J. Abdollahzadeh, V.P. Abreu, K. Adamčíková, G.M.R. Albuquerque, A.V. Alexandrova, E. Álvarez Duarte, C. Armstrong-Cho, S. Banniza, R.N. Barbosa, J.-M. Bellanger, J.L. Bezerra, T.S. Cabral, M. Caboň, E. Caicedo, T. Cantillo, A.J. Carnegie, L.T. Carmo, R.F. Castañeda-Ruiz, C.R. Clement, A. Čmoková, L.B. Conceição, R.H.S.F. Cruz, U. Damm, B.D.B. da Silva, G.A. da Silva, R.M.F. da Silva, A.L.C.M. de A. Santiago, L.F. de Oliveira, C.A.F. de Souza, F. Déniel, B. Dima, G. Dong, J. Edwards, C.R. Félix, J. Fournier, T.B. Gibertoni, K. Hosaka, T. Iturriaga, M. Jadan, J.-L. Jany, Ž. Jurjević, M. Kolařík, I. Kušan, M.F. Landell, T.R. Leite Cordeiro, D.X. Lima, M. Loizides, S. Luo, A.R. Machado, H. Madrid, O.M.C. Magalhães, P. Marinho, N. Matočec, A. Mešić, A.N. Miller, O.V. Morozova, R.P. Neves, K. Nonaka, A. Nováková, N.H. -
Molecular Identification of Fungi
Molecular Identification of Fungi Youssuf Gherbawy l Kerstin Voigt Editors Molecular Identification of Fungi Editors Prof. Dr. Youssuf Gherbawy Dr. Kerstin Voigt South Valley University University of Jena Faculty of Science School of Biology and Pharmacy Department of Botany Institute of Microbiology 83523 Qena, Egypt Neugasse 25 [email protected] 07743 Jena, Germany [email protected] ISBN 978-3-642-05041-1 e-ISBN 978-3-642-05042-8 DOI 10.1007/978-3-642-05042-8 Springer Heidelberg Dordrecht London New York Library of Congress Control Number: 2009938949 # Springer-Verlag Berlin Heidelberg 2010 This work is subject to copyright. All rights are reserved, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilm or in any other way, and storage in data banks. Duplication of this publication or parts thereof is permitted only under the provisions of the German Copyright Law of September 9, 1965, in its current version, and permission for use must always be obtained from Springer. Violations are liable to prosecution under the German Copyright Law. The use of general descriptive names, registered names, trademarks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. Cover design: WMXDesign GmbH, Heidelberg, Germany, kindly supported by ‘leopardy.com’ Printed on acid-free paper Springer is part of Springer Science+Business Media (www.springer.com) Dedicated to Prof. Lajos Ferenczy (1930–2004) microbiologist, mycologist and member of the Hungarian Academy of Sciences, one of the most outstanding Hungarian biologists of the twentieth century Preface Fungi comprise a vast variety of microorganisms and are numerically among the most abundant eukaryotes on Earth’s biosphere. -
UNIVERSIDADE DE BRASÍLIA – Unb
UNIVERSIDADE DE BRASÍLIA – UnB Faculdade de Medicina – FM Pós-Graduação em Patologia Molecular ROBSON WILLIAN DE MELO MATOS O SISTEMA CELULOLÍTICO DE Penicillium echinulatum: ANÁLISE DA ULTRAESTRUTURA MICELIANA E INFLUÊNCIA DE MODULADORES EPIGENÉTICOS Brasília - DF 2012 UNIVERSIDADE DE BRASÍLIA – UnB Faculdade de Medicina – FM Pós-Graduação em Patologia Molecular ROBSON WILLIAN DE MELO MATOS O SISTEMA CELULOLÍTICO DE Penicillium echinulatum: ANÁLISE DA ULTRAESTRUTURA MICELIANA E INFLUÊNCIA DE MODULADORES EPIGENÉTICOS Dissertação de mestrado apresentada ao Departamento de Patologia Molecular, da Faculdade de Medicina, da Universidade de Brasília, como parte dos requisitos para a obtenção do título de mestre em Patologia Molecular. Orientador: Prof. Dr. Marcio José Poças Fonseca Brasília, DF 2012 ii Trabalho desenvolvido no Laboratório de Biologia Molecular, da Universidade de Brasília (UnB), sob a orientação do Prof. Marcio José Poças Fonseca. iii BANCA EXAMINADORA Titulares: Dr. Edivaldo Ximenes Ferreira Filho Departamento de Biologia Celular (CEL/IB) Universidade de Brasília – UnB Dr. Ricardo Bentes de Azevedo Departamento de Genética e Morfologia (GEM/IB) Universidade de Brasília- UnB Orientador: Dr. Marcio José Poças Fonseca Departamento de Genética e Morfologia (GEM/IB) Universidade de Brasília – UnB Suplente: Dra. Ildinete Silva Pereira Departamento de Biologia Celular (CEL/IB) Universidade de Brasília – UnB iv “Feliz daquele que encontra um amigo digno desse nome.” (Menandro) “Quem supera, vence.” (Johann Wolfgang von Goethe) v À minha família e amigos. vi AGRADECIMENTOS Primeiramente, devo muitos agradecimentos aos meus pais, Roberto e Antonieta (in memoriam), e aos meus irmãos, Rodrigo e Rogério, pelo amor, carinho e atenção. Peço desculpas a todos pelos meus destemperos ocasionais e pelas minhas ausências. -
Aspergillus Serratalhadensis Fungal Planet Description Sheets 263
262 Persoonia – Volume 40, 2018 Aspergillus serratalhadensis Fungal Planet description sheets 263 Fungal Planet 720 – 13 July 2018 Aspergillus serratalhadensis L.F. Oliveira, R.N. Barbosa, G.M.R. Albuquerque, Souza-Motta, Viana Marques, sp. nov. Etymology. serratalhadensis, refers to the Brazilian city Serra Talhada, new species Aspergillus serratalhadensis is a distinct lineage the location of the ex-type strain of this species. which belongs to Aspergillus section Nigri, clustering in the Classification — Aspergillaceae, Eurotiales, Eurotiomycetes. A. aculeatus clade. The BLASTn analysis showed low similar- ity of BenA sequences: A. aculeatus (GenBank HE577806.1; On MEA: Stipes brown, smooth, (200–)250–400(–500) × 8– 93 %) and A. brunneoviolaceus (GenBank EF661105.1; 92 %). 9(–10) μm; conidial heads pale to dark brown; uniseriate; vesicle For CmD low similarities were found to A. aculeatus (Gen- subglobose to globose, (32–)50 × 50(–42) μm diam; phialides Bank FN594542.1; 90 %) and A. brunneoviolaceus (GenBank flask-shaped and covering the entire surface of the vesicle, EF661147.1; 90 %). Aspergillus serratalhadensis and these measuring (1.5–)2 × 1.5(–2) µm; conidia globose occasionally two species are uniseriate. However, in A. brunneoviolaceus subglobose, rough-walled to echinulate, brown-black in mass, the conidia are globose to ellipsoidal, smooth, slightly rough- 5(–6.5) μm diam including ornamentation. ened, 3.5–4.5(–6) × 3.5–4.5(–5) μm diam, with a spherical Culture characteristics — (in the dark, 25 °C after 7 d): Colo- vesicle, (30–)35–70(–90) μm diam. In A. aculeatus conidia nies on MEA 54–56 mm diam, sporulating dark brown to black, were spherical, smooth, slightly roughened, 4.9–5.4 μm diam, mycelium white, floccose, exudate absent, no soluble pigments, with a spherical vesicle, 60–63 μm diam (Klich 2002, Jurjević reverse brownish to buff. -
Safety of the Fungal Workhorses of Industrial Biotechnology: Update on the Mycotoxin and Secondary Metabolite Potential of Asper
View metadata,Downloaded citation and from similar orbit.dtu.dk papers on:at core.ac.uk Mar 29, 2019 brought to you by CORE provided by Online Research Database In Technology Safety of the fungal workhorses of industrial biotechnology: update on the mycotoxin and secondary metabolite potential of Aspergillus niger, Aspergillus oryzae, and Trichoderma reesei Frisvad, Jens Christian; Møller, Lars L. H.; Larsen, Thomas Ostenfeld; Kumar, Ravi; Arnau, Jose Published in: Applied Microbiology and Biotechnology Link to article, DOI: 10.1007/s00253-018-9354-1 Publication date: 2018 Document Version Publisher's PDF, also known as Version of record Link back to DTU Orbit Citation (APA): Frisvad, J. C., Møller, L. L. H., Larsen, T. O., Kumar, R., & Arnau, J. (2018). Safety of the fungal workhorses of industrial biotechnology: update on the mycotoxin and secondary metabolite potential of Aspergillus niger, Aspergillus oryzae, and Trichoderma reesei. Applied Microbiology and Biotechnology, 102(22), 9481-9515. DOI: 10.1007/s00253-018-9354-1 General rights Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights. Users may download and print one copy of any publication from the public portal for the purpose of private study or research. You may not further distribute the material or use it for any profit-making activity or commercial gain You may freely distribute the URL identifying the publication in the public portal If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim. -
Gits-Muselli Et Al 2021.Pdf
Different repartition of the cryptic species of black aspergilli according to the anatomical sites in human infections, in a French University hospital Maud Gits-Muselli, Samia Hamane, Benjamin Verillaud, Elisa Cherpin, Blandine Denis, Louise Bondeelle, Sophie Touratier, Alexandre Alanio, Dea Garcia-Hermoso, Stéphane Bretagne To cite this version: Maud Gits-Muselli, Samia Hamane, Benjamin Verillaud, Elisa Cherpin, Blandine Denis, et al.. Dif- ferent repartition of the cryptic species of black aspergilli according to the anatomical sites in hu- man infections, in a French University hospital. Medical Mycology, Oxford University Press, 2021, pp.myab027. 10.1093/mmy/myab027. pasteur-03260950 HAL Id: pasteur-03260950 https://hal-pasteur.archives-ouvertes.fr/pasteur-03260950 Submitted on 15 Jun 2021 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Copyright Medical Mycology, 2021, 0, 1–8 doi:10.1093/mmy/myab027 Advance Access Publication Date: 0 2021 Original Article Original Article Downloaded from https://academic.oup.com/mmy/advance-article/doi/10.1093/mmy/myab027/6281458 -
Effectiveness of 7.5 Percent Povidone Iodine in Comparison to 1 Percent Clotrimazole in the Treatment of Otomycosis
EFFECTIVENESS OF 7.5 PERCENT POVIDONE IODINE IN COMPARISON TO 1 PERCENT CLOTRIMAZOLE IN THE TREATMENT OF OTOMYCOSIS A DISSERTATION SUBMITTED IN PARTIAL FULFILLMENT OF M.S BRANCH –IV (OTORHINOLARYNGOLOGY EXAMINATION OF THE DR.MGR. MEDICAL UNIVERSITY TO BE HELD IN APRIL 2012 ACKNOWLEDGEMENTS I wish to express my deep gratitude to Dr Anand Job, Professor and Head of Unit 1, Department of Otorhinolaryngology, Speech and Hearing, Christian Medical College and Hospital, Vellore for his able guidance and encouragement in conducting this study and preparing this dissertation. I wish to express my deep gratitude to Dr Achamma Balraj, Head of the Department of Otorhinolaryngology, Speech and Hearing, Christian Medical College and Hospital, Vellore for her able guidance and encouragement in conducting this study and preparing this dissertation. I would like to thank Dr Rita Ruby Albert, Dr Regi Thomas, and Dr Rajan Sundaresan from the Department of Otorhinolaryngology for being my co-investigators in this study. I am extremely thankful to Dr Shalini Anandan, Assistant professor, Department of Microbiology for her guidance in this study. I am thankful to Dr Selvaraj from the Department of Biostatistics for his able guidance in the statistical analysis of this study. I would like to thank the Fluid Research Committee, CMC Hospital for granting me financial assistance for conducting this study. Last but not the least; I would like to thank all my patients who participated with me in this study for their kind co-operation. CERTIFICATE This is to certify that the dissertation entitled “Effectiveness of 7.5 percent povidone iodine in comparison to 1 percent clotrimazole in the treatment of otomycosis” is a bonafide original work of Dr Ajay Philip, submitted in partial fulfillment of the rules and regulations for the MS Branch IV, Otorhinolaryngology examination of The Tamil Nadu Dr. -
Lists of Names in Aspergillus and Teleomorphs As Proposed by Pitt and Taylor, Mycologia, 106: 1051-1062, 2014 (Doi: 10.3852/14-0
Lists of names in Aspergillus and teleomorphs as proposed by Pitt and Taylor, Mycologia, 106: 1051-1062, 2014 (doi: 10.3852/14-060), based on retypification of Aspergillus with A. niger as type species John I. Pitt and John W. Taylor, CSIRO Food and Nutrition, North Ryde, NSW 2113, Australia and Dept of Plant and Microbial Biology, University of California, Berkeley, CA 94720-3102, USA Preamble The lists below set out the nomenclature of Aspergillus and its teleomorphs as they would become on acceptance of a proposal published by Pitt and Taylor (2014) to change the type species of Aspergillus from A. glaucus to A. niger. The central points of the proposal by Pitt and Taylor (2014) are that retypification of Aspergillus on A. niger will make the classification of fungi with Aspergillus anamorphs: i) reflect the great phenotypic diversity in sexual morphology, physiology and ecology of the clades whose species have Aspergillus anamorphs; ii) respect the phylogenetic relationship of these clades to each other and to Penicillium; and iii) preserve the name Aspergillus for the clade that contains the greatest number of economically important species. Specifically, of the 11 teleomorph genera associated with Aspergillus anamorphs, the proposal of Pitt and Taylor (2014) maintains the three major teleomorph genera – Eurotium, Neosartorya and Emericella – together with Chaetosartorya, Hemicarpenteles, Sclerocleista and Warcupiella. Aspergillus is maintained for the important species used industrially and for manufacture of fermented foods, together with all species producing major mycotoxins. The teleomorph genera Fennellia, Petromyces, Neocarpenteles and Neopetromyces are synonymised with Aspergillus. The lists below are based on the List of “Names in Current Use” developed by Pitt and Samson (1993) and those listed in MycoBank (www.MycoBank.org), plus extensive scrutiny of papers publishing new species of Aspergillus and associated teleomorph genera as collected in Index of Fungi (1992-2104). -
Mikrobiologiczna Utylizacja Celulozy 2016, 55, 2, 132–146
POST. MIKROBIOL., MIKROBIOLOGICZNA UTYLIZACJA CELULOZY 2016, 55, 2, 132–146 http://www.pm.microbiology.pl Krzysztof Poszytek1* 1 Pracownia Analizy Skażeń Środowiska, Wydział Biologii, Uniwersytet Warszawski Wpłynęło w maju 2015 r. Zaakceptowano w grudniu 2015 r. 1. Wprowadzenie. 2. Charakterystyka celulozy. 3. Mikroorganizmy celulolityczne. 4. Enzymy celulolityczne. 4.1. Podział systemów celulolitycznych. 4.2. Zasady funkcjonowania wolnych i skompleksowanych enzymów celulolitycznych. 4.3. Biologia molekularna oraz inżynieria genetyczna celulaz. 5. Ekologiczny i praktyczny aspekt utylizacji celulozy. 6. Podsumowanie Microbial cellulose utilization Abstract: Lignocellulosic biomass, consisting of lignin, cellulose and hemicellulose, can be utilized as a substrate in the production of biofuels. Before application, lignocellulosic material requires preliminary treatment. Biological pretreatment, which can be an alternative to the physical and chemical methods, is based on the activity of microorganisms, mainly bacteria and fungi. They produce cellulolytic enzymes, cellulases, which can effectively degrade lignocellulosic biomass and other materials containing cellulose. At least three major groups of cellulases are involved in the hydrolysis process: endoglucanases, exoglucanases and β-glucosidases. Various types of cellulases exist in a free form or as complexes, known as cellulosomes. In order to increase the activity, cellulolytic enzymes can be modified by means of genetic engineering. The final results are intended to increase the efficiency of hydrolysis of lignocellulosic biomass and thus the process of biochemical changes in the context of biofuel production. 1. Introduction. 2. Characteristics of cellulose. 3. Cellulolytic microorganisms. 4. Cellulolytic enzymes. 4.1. Classification of cellulolytic enzymes. 4.2. Operating principles of free and complexed cellulolytic enzymes. 4.3. Molecular biology and genetic engineering of cellulases. -
Production of Extracellular Enzymes and Degradation of Biopolymers by Saprotrophic Microfungi from the Upper Layers of Forest Soil
Plant Soil (2011) 338:111–125 DOI 10.1007/s11104-010-0324-3 REGULAR ARTICLE Production of extracellular enzymes and degradation of biopolymers by saprotrophic microfungi from the upper layers of forest soil Petr Baldrian & Jana Voříšková & Petra Dobiášová & Věra Merhautová & Ludmila Lisá & Vendula Valášková Received: 14 October 2009 /Accepted: 10 February 2010 /Published online: 26 February 2010 # Springer Science+Business Media B.V. 2010 Abstract Production of extracellular enzymes partic- The microfungal strains were able to produce signif- ipating in the degradation of biopolymers was studied icant growth on a range of 41–87, out of 95 simple C- in 29 strains of nonbasidiomycetous microfungi containing substrates tested in a Biolog™ assay, isolated from Quercus petraea forest soil based on monosaccharides being for all strains the most rapidly the frequency of occurrence. Most of the isolates were metabolized C-sources. Comparison with saprotro- ascomycetes and belonged to the genera Acremonium, phic basidiomycetes from the same environment Alternaria, Cladosporium, Geomyces, Hypocrea, showed that microfungi have similar cellulolytic Myrothecium, Ochrocladosporium, and Penicillium capabilities and higher chitinase activities which (18 isolates), and two isolates were zygomycetes. Only testifies for their active role in the decomposition of six isolates showed phenol oxidation activity which both lignocellulose and dead fungal biomass, impor- was low and none of the strains were able to degrade tant pools of soil carbon. humic acids. Approximately half of the strains were able to degrade cellulose and all but six degraded Keywords Lignocellulose . Soil microfungi . Chitin . chitin. Most strains produced significant amounts of Enzymes . Decomposition . Forest biogeochemistry the cellulolytic enzymes cellobiohydrolase and β- glucosidase and the chitinolytic enzymes chitinase, Abbreviations chitobiosidase, and N-acetylglucosaminidase. -
207-219 44(4) 01.홍승범R.Fm
한국균학회지 The Korean Journal of Mycology Review 일균일명 체계에 의한 국내 보고 Aspergillus, Penicillium, Talaromyces 속의 종 목록 정리 김현정 1† · 김정선 1† · 천규호 1 · 김대호 2 · 석순자 1 · 홍승범 1* 1국립농업과학원 농업미생물과 미생물은행(KACC), 2강원대학교 산림환경과학대학 산림환경보호학과 Species List of Aspergillus, Penicillium and Talaromyces in Korea, Based on ‘One Fungus One Name’ System 1† 1† 1 2 1 1 Hyeon-Jeong Kim , Jeong-Seon Kim , Kyu-Ho Cheon , Dae-Ho Kim , Soon-Ja Seok and Seung-Beom Hong * 1 Korean Agricultural Culture Collection, Agricultural Microbiology Division National Institute of Agricultural Science, Wanju 55365, Korea 2 Tree Pathology and Mycology Laboratory, Department of Forestry and Environmental Systems, Kangwon National University, Chun- cheon 24341, Korea ABSTRACT : Aspergillus, Penicillium, and their teleomorphic genera have a worldwide distribution and large economic impacts on human life. The names of species in the genera that have been reported in Korea are listed in this study. Fourteen species of Aspergillus, 4 of Eurotium, 8 of Neosartorya, 47 of Penicillium, and 5 of Talaromyces were included in the National List of Species of Korea, Ascomycota in 2015. Based on the taxonomic system of single name nomenclature on ICN (International Code of Nomenclature for algae, fungi, and plants), Aspergillus and its teleomorphic genera such as Neosartorya, Eurotium, and Emericella were named as Aspergillus and Penicillium, and its teleomorphic genera such as Eupenicillium and Talaromyces were named as Penicillium (subgenera Aspergilloides, Furcatum, and Penicillium) and Talaromyces (subgenus Biverticillium) in this study. In total, 77 species were added and the revised list contains 55 spp. of Aspergillus, 82 of Penicillium, and 18 of Talaromyces.