Aspergillus Serratalhadensis Fungal Planet Description Sheets 263

262 Persoonia – Volume 40, 2018

Aspergillus serratalhadensis Fungal Planet description sheets 263

Fungal Planet 720 – 13 July 2018 Aspergillus serratalhadensis L.F. Oliveira, R.N. Barbosa, G.M.R. Albuquerque, Souza-Motta, Viana Marques, sp. nov. Etymology. serratalhadensis, refers to the Brazilian city Serra Talhada, new species Aspergillus serratalhadensis is a distinct lineage the location of the ex-type strain of this species. which belongs to Aspergillus section Nigri, clustering in the Classiﬁcation — Aspergillaceae, Eurotiales, Eurotiomycetes. A. aculeatus clade. The BLASTn analysis showed low similar- ity of BenA sequences: A. aculeatus (GenBank HE577806.1; On MEA: Stipes brown, smooth, (200–)250–400(–500) × 8– 93 %) and A. brunneoviolaceus (GenBank EF661105.1; 92 %). 9(–10) μm; conidial heads pale to dark brown; uniseriate; vesicle For CmD low similarities were found to A. aculeatus (Gen- subglobose to globose, (32–)50 × 50(–42) μm diam; phialides Bank FN594542.1; 90 %) and A. brunneoviolaceus (GenBank flask-shaped and covering the entire surface of the vesicle, EF661147.1; 90 %). Aspergillus serratalhadensis and these measuring (1.5–)2 × 1.5(–2) µm; conidia globose occasionally two species are uniseriate. However, in A. brunneoviolaceus subglobose, rough-walled to echinulate, brown-black in mass, the conidia are globose to ellipsoidal, smooth, slightly rough- 5(–6.5) μm diam including ornamentation. ened, 3.5–4.5(–6) × 3.5–4.5(–5) μm diam, with a spherical Culture characteristics — (in the dark, 25 °C after 7 d): Colo- vesicle, (30–)35–70(–90) μm diam. In A. aculeatus conidia nies on MEA 54–56 mm diam, sporulating dark brown to black, were spherical, smooth, slightly roughened, 4.9–5.4 μm diam, mycelium white, floccose, exudate absent, no soluble pigments, with a spherical vesicle, 60–63 μm diam (Klich 2002, Jurjević reverse brownish to buff. Colonies on CYA 60–68 mm diam, et al. 2012).The new species described also differs in growth dark brown to black, mycelium white, floccose, exudate absent, rate on the various media tested. Aspergillus serratalhadensis no soluble pigments, reverse brownish to buff. Colonies on OA was isolated from soil collected in the Brazilian tropical dry for- 38–40 mm diam, sporulating dark brown to black, mycelium est (Caatinga) in the city of Serra Talhada, Pernambuco state. white to pale, floccose, exudate absent, no soluble pigments, reverse darkness. Colonies on YES 60–65 mm diam, sporu- Aspergillus piperis CBS 112811 lating dark brown to black, mycelium white, floccose, sulcate, 1 exudate absent, no soluble pigments, reverse pale. Colonies Aspergillus luchuensis CBS 205.80 1 on CY20S 60–65 mm diam, with black sporulation, mycelium Aspergillus costaricaensis CBS 115574 A. tubingensis 0.53 white, floccose, no exudate, no soluble pigments, reverse pale Aspergillus eucalypticola CBS 122712 clade 0.86 Aspergillus vadensis CBS 113365 to pale buff. Colonies on CREA growing more slowly compared A. niger 1 with other media, 19–20 mm diam, poor sporulation, mycelium Aspergillus neoniger CBS 115656 aggregate white, production of acid positive. No growth on MEA and CYA 0.98 Aspergillus tubingensis NRRL 4875 Aspergillus niger CBS 554.65 at 37 °C. 1 1 A. niger/welwitschiae Aspergillus welwitschiae CBS 139.54 clade Typus. Brazil, Pernambuco state, Serra Talhada, S7°57'21" W38°17'34", Aspergillus brasiliensis CBS 101740 isolated from soil, Sept. 2015, L.F. Oliveira (holotype URM 91189, ex-type 1 Aspergillus sclerotiicarbonarius CBS 121057 culture URM 7866, ITS, BenA, CmD and RPB2 sequences GenBank 0.79 Aspergillus ibericus CBS 35644 MH169127, LT993222, LT993223 and LT995971, MycoBank MB824978). 1 A.carbonarius 1 Aspergillus carbonarius CBS 111.26 clade 1 Notes — ITS, CmD and BenA sequences are important Aspergillus sclerotioniger CBS 115572 Aspergillus ellipticus CBS 482.65 identiﬁcation markers for Aspergillus (Fungaro et al. 2017, Sam- 1 A. heteromorphus clade son et al. 2014). Based on the current phylogenetic analysis, the Aspergillus heteromorphus CBS 117.55 Aspergillus aculeatinus CBS 121060 1 Aspergillus trinidadensis NRRL 62479 1 Aspergillus fijiensis CBS 313.89

1 1 1 Aspergillus brunneoviolaceus CBS 621.78 0.83 Aspergillus aculeatus NRRL 5094 A. aculeatus clade 1 Aspergillus floridensis NRRL 62478 Aspergillus indologenus CBS 114.80 1 1 1 Aspergillus japonicus CBS 114.51 Aspergillus uvarum CBS 121591 1 Aspergillus serratalhadensis sp.nov Aspergillus homomorphus CBS 101889 1 0.54 A. homomorphus Aspergillus saccharolyticus CBS 127449 clade Aspergillus labruscus ITAL 22.223 0.05 Aspergillus flavus CBS 569.65 Bayesian inference tree obtained by phylogenetic analyses of the combined ITS, BenA and CmD sequences conducted in MrBayes on XSEDE in the CIPRES science gateway. Bayes- ian posterior probability values are indicated at the nodes. The Colour illustrations. Caatinga’s soil, isolation source of Aspergillus ser new species is indicated in face. Aspergillus flavus (CBS ratalhadensis; conidia; conidiophores from 7-d-old colonies on MEA. Scale bold bars = 10 µm. 569.65) was used as outgroup.

Luiz F. de Oliveira & Daniela de A. Viana Marques, Universidade de Pernambuco- Campus Serra Talhada, Serra Talhada, Brazil; e-mail: [email protected], [email protected] Renan N. Barbosa, Greicilene M.R. Albuquerque & Cristina M. Souza-Motta, URM Culture Collection, Universidade Federal de Pernambuco, Recife, Brazil; e-mail: [email protected], [email protected] & [email protected]