Citric Acid and Itaconic Acid Accumulation: Variations of the Same Story?
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Design and Development of Molecularly Imprinted Polymers and Imprinted Sensors
ADVERTIMENT. Lʼaccés als continguts dʼaquesta tesi queda condicionat a lʼacceptació de les condicions dʼús establertes per la següent llicència Creative Commons: http://cat.creativecommons.org/?page_id=184 ADVERTENCIA. El acceso a los contenidos de esta tesis queda condicionado a la aceptación de las condiciones de uso establecidas por la siguiente licencia Creative Commons: http://es.creativecommons.org/blog/licencias/ WARNING. The access to the contents of this doctoral thesis it is limited to the acceptance of the use conditions set by the following Creative Commons license: https://creativecommons.org/licenses/?lang=en Design and development of molecularly imprinted polymers and imprinted sensors Ferdia Bates Doctoral Thesis Doctoral Studies in Chemistry Director: Manel del Valle Zafra Departament de Química Facultat de Ciències 2016 Declaration Thesis submitted to aspire for the doctoral degree Ferdia Bates Director's approval: Dr. Manel de Valle Zafra Professor of Analytical Chemistry Bellaterra (Ceerdanyola del Vallès), September 2016 III Funding acknowledgement This present dissertation has been carried out in the laboratories of the Grup de Sensors i Biosensors of the Department de Química in the Universitat Autònoma de Barcelona, with the support of the Marie Curie Actions fellowship FP7-PEOPLE-2010-ITN- and the financial support of the Ministry of Economy and Innovation (MINECO) project “Electronic tongue fingerprinting: aplicaciones en el campo alimentario y de seguridad” (MCINN, CTQ2013-41577-P). Grup de Senors i Biosensors Unitat de Química Analítica Department de Química Universitat Autónoma de Barcelona Edifici Cn 08193, Bellatera IV Acknowledgments For my Masters supervisor at Cranfield university, Doctor Yi Ge, my respected colleague and friend, I would like to offer my most heart-felt thanks; without Dr Ge's encouragement, advise and reference, I most probably would not have pursued a doctorate as a career choice. -
Distribution of Methionine Sulfoxide Reductases in Fungi and Conservation of the Free- 2 Methionine-R-Sulfoxide Reductase in Multicellular Eukaryotes
bioRxiv preprint doi: https://doi.org/10.1101/2021.02.26.433065; this version posted February 27, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 1 Distribution of methionine sulfoxide reductases in fungi and conservation of the free- 2 methionine-R-sulfoxide reductase in multicellular eukaryotes 3 4 Hayat Hage1, Marie-Noëlle Rosso1, Lionel Tarrago1,* 5 6 From: 1Biodiversité et Biotechnologie Fongiques, UMR1163, INRAE, Aix Marseille Université, 7 Marseille, France. 8 *Correspondence: Lionel Tarrago ([email protected]) 9 10 Running title: Methionine sulfoxide reductases in fungi 11 12 Keywords: fungi, genome, horizontal gene transfer, methionine sulfoxide, methionine sulfoxide 13 reductase, protein oxidation, thiol oxidoreductase. 14 15 Highlights: 16 • Free and protein-bound methionine can be oxidized into methionine sulfoxide (MetO). 17 • Methionine sulfoxide reductases (Msr) reduce MetO in most organisms. 18 • Sequence characterization and phylogenomics revealed strong conservation of Msr in fungi. 19 • fRMsr is widely conserved in unicellular and multicellular fungi. 20 • Some msr genes were acquired from bacteria via horizontal gene transfers. 21 1 bioRxiv preprint doi: https://doi.org/10.1101/2021.02.26.433065; this version posted February 27, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. -
Itaconic Acid Production by Microorganisms: a Review
Current Research Journal of Biological Sciences 7(2): 37-42, 2015 ISSN: 2041-076X, e-ISSN: 2041-0778 © Maxwell Scientific Organization, 2015 Submitted: September 29, 2014 Accepted: November 26, 2014 Published: April 20, 2015 Itaconic Acid Production by Microorganisms: A Review Helia Hajian and Wan Mohtar Wan Yusoff School of Bioscience and Biotechnology, Faculty of Science and Technologi, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia Abstract: Itaconic acid (C5H6O4) is an organic acid with unique structure and characteristics. In order to promote the bio-based economy, the US-Department of Energy (DOE) assigned a “top-12” of platform chemicals, which include numerous of organic acids. In particular di-carboxylic acids, like itaconic acid, can be used as monomers for bio-polymers. Thus the need to produce itaconic acid attracts much attention. The favored production process is fermentation of carbohydrates by fungi and Aspergillus terreus is the mostly frequently employed commercial producer of itaconic acid. This review reports the current status of use of microorganisms in enhancing productivity. Keywords: Aspergillus, fermentation, itaconic acid, production INTRODUCTION on production of itaconic acid from renewable resources, a drastic shift from the currently prevalent Itaconic acid (methylene succinic acid) is a sourcing from petrochemical feedstock. Asia-Pacific, promising organic acid. It is a white crystalline backed by tremendous impetus from China is poised to unsaturated dicarbonic acid in which one carboxyl emerge as the fastest growing market for itaconic acid group is conjugated to the methylene group. Different at a Compound Annual Growth rate (CAGR) of over microorganisms have been used in industry for the 9.0% through 2017. -
Fungal Planet Description Sheets: 716–784 By: P.W
Fungal Planet description sheets: 716–784 By: P.W. Crous, M.J. Wingfield, T.I. Burgess, G.E.St.J. Hardy, J. Gené, J. Guarro, I.G. Baseia, D. García, L.F.P. Gusmão, C.M. Souza-Motta, R. Thangavel, S. Adamčík, A. Barili, C.W. Barnes, J.D.P. Bezerra, J.J. Bordallo, J.F. Cano-Lira, R.J.V. de Oliveira, E. Ercole, V. Hubka, I. Iturrieta-González, A. Kubátová, M.P. Martín, P.-A. Moreau, A. Morte, M.E. Ordoñez, A. Rodríguez, A.M. Stchigel, A. Vizzini, J. Abdollahzadeh, V.P. Abreu, K. Adamčíková, G.M.R. Albuquerque, A.V. Alexandrova, E. Álvarez Duarte, C. Armstrong-Cho, S. Banniza, R.N. Barbosa, J.-M. Bellanger, J.L. Bezerra, T.S. Cabral, M. Caboň, E. Caicedo, T. Cantillo, A.J. Carnegie, L.T. Carmo, R.F. Castañeda-Ruiz, C.R. Clement, A. Čmoková, L.B. Conceição, R.H.S.F. Cruz, U. Damm, B.D.B. da Silva, G.A. da Silva, R.M.F. da Silva, A.L.C.M. de A. Santiago, L.F. de Oliveira, C.A.F. de Souza, F. Déniel, B. Dima, G. Dong, J. Edwards, C.R. Félix, J. Fournier, T.B. Gibertoni, K. Hosaka, T. Iturriaga, M. Jadan, J.-L. Jany, Ž. Jurjević, M. Kolařík, I. Kušan, M.F. Landell, T.R. Leite Cordeiro, D.X. Lima, M. Loizides, S. Luo, A.R. Machado, H. Madrid, O.M.C. Magalhães, P. Marinho, N. Matočec, A. Mešić, A.N. Miller, O.V. Morozova, R.P. Neves, K. Nonaka, A. Nováková, N.H. -
|||||||III US005457040A United States Patent (19) 11) Patent Number: 5,457,040 Jarry Et Al
|||||||III US005457040A United States Patent (19) 11) Patent Number: 5,457,040 Jarry et al. (45) Date of Patent: Oct. 10, 1995 (54) PRODUCTION OF ITACONIC ACID BY 4,740,464 4/1988 Holdom et al.......................... 435/135 FERMENTATION 5,231,016 7/1993 Cros et al. .............................. 435/142 (75) Inventors: Alain Jarry, Maisonnay; Yolaine FOREIGN PATENT DOCUMENTS Seraudie, Melle, both of France 0697653 11/1964 Canada .................................. 435/142 0341112 11/1989 European Pat. Off. 73) Assignee: Rhone-Poulenc Chimie, Courbevoie, 1327.937 4/1963 France. France 0052990 7/1973 Japan ..................................... 435/142 0507633 3/1976 U.S.S.R. ................ ... 435/142 0602866 6/1948 United Kingdom....... ... 435/142 (21 Appl. No.: 205,646 0795401 5/1958 United Kingdom....... ... 435/142 22 Filed: Mar. 4, 1994 0878152 9/1961 United Kingdom ................... 435/142 Primary Examiner-Herbert J. Lilling (30) Foreign Application Priority Data Attorney, Agent, or Firm-Burns, Doane, Swecker & Mathis Mar. 12, 1993 (FR) France ................................... 93 02844 57) ABSTRACT (51) Int. Cl. ............................................ C12P 7/44 Itaconic acid and/or salt thereof is produced via aerobic 52 U.S. Cl. ................ 435/142; 435/913 microbial fermentation, for example by means of the species 58) Field of Search .......... - - - - - 435/142,913 Aspergillus terreus or Aspergillus itaconicus, of a nutrient medium containing a source of assimilable carbon, such 56) References Cited carbon source at least in part comprising an effective amount U.S. PATENT DOCUMENTS of glycerol. 3,873,425 3/1975 Kobayashi et al. ..................... 435/145 1 Claim, No Drawings 5,457,040 1. 2 PRODUCTION OF TACONCACD BY the other carbon substrates indicated above, glycerol pre FERMENTATION sents the distinct advantage of being especially advanta geous from an economic standpoint. -
Modeling of Overflow Metabolism in Batch and Fed-Batch Cultures of Escherichia Coli
Biotechnol. Prog. 1999, 15, 81−90 81 Modeling of Overflow Metabolism in Batch and Fed-Batch Cultures of Escherichia coli Bo Xu, Mehmedalija Jahic, and Sven-Olof Enfors* Department of Biotechnology, Royal Institute of Technology, S-100 44 Stockholm, Sweden A dynamic model of glucose overflow metabolism in batch and fed-batch cultivations of Escherichia coli W3110 under fully aerobic conditions is presented. Simulation based on the model describes cell growth, respiration, and acetate formation as well as acetate reconsumption during batch cultures, the transition of batch to fed-batch culture, and fed-batch cultures. E. coli excreted acetate only when specific glucose uptake exceeded a critical rate corresponding to a maximum respiration rate. In batch cultures where the glucose uptake was unlimited, the overflow acetate made up to 9.0 ( 1.0% carbon/ carbon of the glucose consumed. The applicability of the model to dynamic situations was tested by challenging the model with glucose and acetate pulses added during the fed-batch part of the cultures. In the presence of a glucose feed, E. coli utilized acetate 3 times faster than in the absence of glucose. The cells showed no significant difference in maximum specific uptake rate of endogenous acetate produced by glucose overflow and exogenous acetate added to the culture, the value being 0.12-0.18 g g-1 h-1 during the entire fed-batch culture period. Acetate inhibited the specific growth rate according to a noncompetitive model, with the inhibition constant (ki) being 9 g of acetate/L. This was due to the reduced rate of glucose uptake rather than the reduced yield of biomass. -
Regulation of Amino-Acid Metabolism Controls Flux to Lipid Accumulation in Yarrowia Lipolytica
View metadata,Downloaded citation and from similar orbit.dtu.dk papers on:at core.ac.uk Nov 09, 2017 brought to you by CORE provided by Online Research Database In Technology Regulation of amino-acid metabolism controls flux to lipid accumulation in Yarrowia lipolytica Kerkhoven, Eduard J.; Pomraning, Kyle R.; Baker, Scott E.; Nielsen, Jens Published in: n p j Systems Biology and Applications Link to article, DOI: 10.1038/npjsba.2016.5 Publication date: 2016 Document Version Publisher's PDF, also known as Version of record Link back to DTU Orbit Citation (APA): Kerkhoven, E. J., Pomraning, K. R., Baker, S. E., & Nielsen, J. (2016). Regulation of amino-acid metabolism controls flux to lipid accumulation in Yarrowia lipolytica. n p j Systems Biology and Applications, 2, [16005]. DOI: 10.1038/npjsba.2016.5 General rights Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights. • Users may download and print one copy of any publication from the public portal for the purpose of private study or research. • You may not further distribute the material or use it for any profit-making activity or commercial gain • You may freely distribute the URL identifying the publication in the public portal If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim. www.nature.com/npjsba All rights reserved 2056-7189/16 ARTICLE OPEN Regulation of amino-acid metabolism controls flux to lipid accumulation in Yarrowia lipolytica Eduard J Kerkhoven1, Kyle R Pomraning2, Scott E Baker2 and Jens Nielsen1,3 Yarrowia lipolytica is a promising microbial cell factory for the production of lipids to be used as fuels and chemicals, but there are few studies on regulation of its metabolism. -
Isolation and Identification of Microfungi from Soils in Serdang, Selangor, Malaysia Article
Studies in Fungi 5(1): 6–16 (2020) www.studiesinfungi.org ISSN 2465-4973 Article Doi 10.5943/sif/5/1/2 Isolation and identification of microfungi from soils in Serdang, Selangor, Malaysia Mohd Nazri NIA1, Mohd Zaini NA1, Aris A1, Hasan ZAE1, Abd Murad NB1, 2 1 Yusof MT and Mohd Zainudin NAI 1 Department of Biology, Faculty of Science, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia 2 Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia Mohd Nazri NIA, Mohd Zaini NA, Aris A, Hasan ZAE, Abd Murad NB, Yusof MT, Mohd Zainudin NAI 2020 – Isolation and identification of microfungi from soils in Serdang, Selangor, Malaysia. Studies in Fungi 5(1), 6–16, Doi 10.5943/sif/5/1/2 Abstract Microfungi are commonly inhabited soil with various roles. The present study was conducted in order to isolate and identify microfungi from soil samples in Serdang, Selangor, Malaysia. In this study, the soil microfungi were isolated using serial dilution technique and spread plate method. A total of 25 isolates were identified into ten genera based on internal transcribed spacer region (ITS) sequence analysis, namely Aspergillus, Clonostachys, Colletotrichum, Curvularia, Gliocladiopsis, Metarhizium, Myrmecridium, Penicillium, Scedosporium and Trichoderma consisting 18 fungi species. Aspergillus and Penicillium species were claimed as predominant microfungi inhabiting the soil. Findings from this study can be used as a checklist for future studies related to fungi distribution in tropical lands. For improving further study, factors including the physicochemical properties of soil and anthropogenic activities in the sampling area should be included. -
Aspergillus Serratalhadensis Fungal Planet Description Sheets 263
262 Persoonia – Volume 40, 2018 Aspergillus serratalhadensis Fungal Planet description sheets 263 Fungal Planet 720 – 13 July 2018 Aspergillus serratalhadensis L.F. Oliveira, R.N. Barbosa, G.M.R. Albuquerque, Souza-Motta, Viana Marques, sp. nov. Etymology. serratalhadensis, refers to the Brazilian city Serra Talhada, new species Aspergillus serratalhadensis is a distinct lineage the location of the ex-type strain of this species. which belongs to Aspergillus section Nigri, clustering in the Classification — Aspergillaceae, Eurotiales, Eurotiomycetes. A. aculeatus clade. The BLASTn analysis showed low similar- ity of BenA sequences: A. aculeatus (GenBank HE577806.1; On MEA: Stipes brown, smooth, (200–)250–400(–500) × 8– 93 %) and A. brunneoviolaceus (GenBank EF661105.1; 92 %). 9(–10) μm; conidial heads pale to dark brown; uniseriate; vesicle For CmD low similarities were found to A. aculeatus (Gen- subglobose to globose, (32–)50 × 50(–42) μm diam; phialides Bank FN594542.1; 90 %) and A. brunneoviolaceus (GenBank flask-shaped and covering the entire surface of the vesicle, EF661147.1; 90 %). Aspergillus serratalhadensis and these measuring (1.5–)2 × 1.5(–2) µm; conidia globose occasionally two species are uniseriate. However, in A. brunneoviolaceus subglobose, rough-walled to echinulate, brown-black in mass, the conidia are globose to ellipsoidal, smooth, slightly rough- 5(–6.5) μm diam including ornamentation. ened, 3.5–4.5(–6) × 3.5–4.5(–5) μm diam, with a spherical Culture characteristics — (in the dark, 25 °C after 7 d): Colo- vesicle, (30–)35–70(–90) μm diam. In A. aculeatus conidia nies on MEA 54–56 mm diam, sporulating dark brown to black, were spherical, smooth, slightly roughened, 4.9–5.4 μm diam, mycelium white, floccose, exudate absent, no soluble pigments, with a spherical vesicle, 60–63 μm diam (Klich 2002, Jurjević reverse brownish to buff. -
Mining of Cryptic Secondary Metabolism in Aspergillus
Downloaded from orbit.dtu.dk on: Oct 10, 2021 Mining of Cryptic Secondary Metabolism in Aspergillus Guo, Yaojie Publication date: 2020 Document Version Publisher's PDF, also known as Version of record Link back to DTU Orbit Citation (APA): Guo, Y. (2020). Mining of Cryptic Secondary Metabolism in Aspergillus. DTU Bioengineering. General rights Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights. Users may download and print one copy of any publication from the public portal for the purpose of private study or research. You may not further distribute the material or use it for any profit-making activity or commercial gain You may freely distribute the URL identifying the publication in the public portal If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim. Mining of Cryptic Secondary Metabolism in Aspergillus O O O N R HN O N N N O O O OH OH O O OH OH OH O OH O OH OH OH OH HO O OH OH O OH OH OH O Yaojie Guo PhD Thesis Department of Biotechnology and Biomedicine Technical University of Denmark September 2020 Mining of Cryptic Secondary Metabolism in Aspergillus Yaojie Guo PhD Thesis Department of Biotechnology and Biomedicine Technical University of Denmark September 2020 Supervisors: Professor Thomas Ostenfeld Larsen Professor Uffe Hasbro Mortensen Preface This thesis is submitted to the Technical University of Denmark (Danmarks Tekniske Universitet, DTU) in partial fulfillment of the requirements for the Degree of Doctor of Philosophy. -
Short-Chain Fatty Acids Modulate Metabolic Pathways and Membrane Lipids in Prevotella Bryantii B14
proteomes Article Short-Chain Fatty Acids Modulate Metabolic Pathways and Membrane Lipids in Prevotella bryantii B14 Andrej Trautmann 1, Lena Schleicher 2, Simon Deusch 1 , Jochem Gätgens 3 , Julia Steuber 2 and Jana Seifert 1,* 1 Institute of Animal Science, University of Hohenheim, 70599 Stuttgart, Germany; [email protected] (A.T.); [email protected] (S.D.) 2 Institute of Biology, University of Hohenheim, 70599 Stuttgart, Germany; [email protected] (L.S.); [email protected] (J.S.) 3 Institute of Bio- and Geosciences, IBG-1: Biotechnology, Forschungszentrum Jülich, 52425 Jülich, Germany; [email protected] * Correspondence: [email protected] Received: 22 September 2020; Accepted: 13 October 2020; Published: 16 October 2020 Abstract: Short-chain fatty acids (SCFAs) are bacterial products that are known to be used as energy sources in eukaryotic hosts, whereas their role in the metabolism of intestinal microbes is rarely explored. In the present study, acetic, propionic, butyric, isobutyric, valeric, and isovaleric acid, respectively, were added to a newly defined medium containing Prevotella bryantii B14 cells. After 8 h and 24 h, optical density, pH and SCFA concentrations were measured. Long-chain fatty acid (LCFA) profiles of the bacterial cells were analyzed via gas chromatography-time of flight-mass spectrometry (GC-ToF MS) and proteins were quantified using a mass spectrometry-based, label-free approach. Cultures supplemented with single SCFAs revealed different growth behavior. Structural features of the respective SCFAs were identified in the LCFA profiles, which suggests incorporation into the bacterial membranes. The proteomes of cultures supplemented with acetic and valeric acid differed by an increased abundance of outer membrane proteins. -
Genomic and Genetic Insights Into a Cosmopolitan Fungus, Paecilomyces Variotii (Eurotiales)
fmicb-09-03058 December 11, 2018 Time: 17:41 # 1 ORIGINAL RESEARCH published: 13 December 2018 doi: 10.3389/fmicb.2018.03058 Genomic and Genetic Insights Into a Cosmopolitan Fungus, Paecilomyces variotii (Eurotiales) Andrew S. Urquhart1, Stephen J. Mondo2, Miia R. Mäkelä3, James K. Hane4,5, Ad Wiebenga6, Guifen He2, Sirma Mihaltcheva2, Jasmyn Pangilinan2, Anna Lipzen2, Kerrie Barry2, Ronald P. de Vries6, Igor V. Grigoriev2 and Alexander Idnurm1* 1 School of BioSciences, University of Melbourne, Melbourne, VIC, Australia, 2 U.S. Department of Energy Joint Genome Institute, Walnut Creek, CA, United States, 3 Department of Microbiology, Faculty of Agriculture and Forestry, Viikki Biocenter 1, University of Helsinki, Helsinki, Finland, 4 CCDM Bioinformatics, Centre for Crop and Disease Management, Curtin University, Bentley, WA, Australia, 5 Curtin Institute for Computation, Curtin University, Bentley, WA, Australia, 6 Fungal Physiology, Westerdijk Fungal Biodiversity Institute and Fungal Molecular Physiology, Utrecht University, Utrecht, Netherlands Species in the genus Paecilomyces, a member of the fungal order Eurotiales, are ubiquitous in nature and impact a variety of human endeavors. Here, the biology of one common species, Paecilomyces variotii, was explored using genomics and functional genetics. Sequencing the genome of two isolates revealed key genome and gene features in this species. A striking feature of the genome was the two-part nature, featuring large stretches of DNA with normal GC content separated by AT-rich regions, Edited by: a hallmark of many plant-pathogenic fungal genomes. These AT-rich regions appeared Monika Schmoll, Austrian Institute of Technology (AIT), to have been mutated by repeat-induced point (RIP) mutations. We developed methods Austria for genetic transformation of P.