Genomic and Genetic Insights Into a Cosmopolitan Fungus, Paecilomyces Variotii (Eurotiales)
fmicb-09-03058 December 11, 2018 Time: 17:41 # 1 ORIGINAL RESEARCH published: 13 December 2018 doi: 10.3389/fmicb.2018.03058 Genomic and Genetic Insights Into a Cosmopolitan Fungus, Paecilomyces variotii (Eurotiales) Andrew S. Urquhart1, Stephen J. Mondo2, Miia R. Mäkelä3, James K. Hane4,5, Ad Wiebenga6, Guifen He2, Sirma Mihaltcheva2, Jasmyn Pangilinan2, Anna Lipzen2, Kerrie Barry2, Ronald P. de Vries6, Igor V. Grigoriev2 and Alexander Idnurm1* 1 School of BioSciences, University of Melbourne, Melbourne, VIC, Australia, 2 U.S. Department of Energy Joint Genome Institute, Walnut Creek, CA, United States, 3 Department of Microbiology, Faculty of Agriculture and Forestry, Viikki Biocenter 1, University of Helsinki, Helsinki, Finland, 4 CCDM Bioinformatics, Centre for Crop and Disease Management, Curtin University, Bentley, WA, Australia, 5 Curtin Institute for Computation, Curtin University, Bentley, WA, Australia, 6 Fungal Physiology, Westerdijk Fungal Biodiversity Institute and Fungal Molecular Physiology, Utrecht University, Utrecht, Netherlands Species in the genus Paecilomyces, a member of the fungal order Eurotiales, are ubiquitous in nature and impact a variety of human endeavors. Here, the biology of one common species, Paecilomyces variotii, was explored using genomics and functional genetics. Sequencing the genome of two isolates revealed key genome and gene features in this species. A striking feature of the genome was the two-part nature, featuring large stretches of DNA with normal GC content separated by AT-rich regions, Edited by: a hallmark of many plant-pathogenic fungal genomes. These AT-rich regions appeared Monika Schmoll, Austrian Institute of Technology (AIT), to have been mutated by repeat-induced point (RIP) mutations. We developed methods Austria for genetic transformation of P.
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