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Supplemental Data For: Fernández González, M., J Supplemental Data for: Fernández González, M., J. Martínez Gascueña, and A. Mena Morales. 2012. Identification and relationships of grapevine cultivars authorized for cultivation in Castilla La Mancha (Spain). Am. J. Enol. Vitic. 63:564-567. doi: 10.5344/ajev.2012.09010. Supplemental Table 1 Cultivars studied, berry color (C), number of samples analyzed (N), and Castilla La Mancha Appellations of Origin (AO) in which they are authorized. Recommended cultivars Ca N AOb Authorized cultivars Ca N AOb Airén B 8 2,3,9 Alarije B 1 Albillo Real B 12 4,5 Cabernet franc N 3 3,4,7,g Bobal N 3 3,4,7 Cabernet Sauvignon N 4 1,2,3,4,5,6,7,8,9,a,b,c,d,e,g,h Coloráillo R 10 Chardonnay B 3 1,2,3,4,5,8,9,a,c,d,e,g Garnacha Tinta N 4 1,2,3,4,5,8,9,h Forcallat Tinta N 1 Garnacha Tintorera N 3 1,2,4 Garnacha Peluda N 3 Macabeo, Viura B 4 2,3,4,5,6,8,9 Gewürtztraminer B 2 3 Malvar B 2 6 Graciano N 2 3,4,b Merseguera B 2 Malbec N 1 4,e,g Monastrell N 2 1,2,3,4 Malvasía Aromática B 1 2 Muscat à petits grains blancs B 5 1,2,3,4,6,7,8,9 Mazuela, Cariñena N 1 4 Pedro Ximénez B 1 2,3 Mencía N 1 3 Tempranillo, Cencibel N 6 1,2,3,4,5,6,7,8,9, Merlot N 4 1,2,3,4,5,6,7,8,9,a,b,c,d,e,g a,c,d,e,f,g,h Tinto Velasco, Frasco N 3 4 Montúa, Chelva B 1 Torrontés B 4 3,6 Moravia Agria N 3 4 Moravia Dulce, Crujidera N 7 3,4 Muscat of Alexandria B 3 Pardillo, Marisancho B 4 4 Pardina, Jaén Blanco B 3 Parellada B 2 3 Petit Verdot N 2 1,2,3,4,5,7,9,b,e,f,g Pinot noir N 1 3,4 Prieto Picudo N 1 Riesling B 3 3 Rojal Tinta R 3 4 Sauvignon blanc B 2 1,2,3,4,5,6,7,9,d,g Syrah N 3 1,2,3,4,5,6,7,8,9,a,b,c,d,e,f,g,h Tinto de la Pámpana Blanca N 3 Verdejo B 2 1,3,4,5,6,8,9 Verdoncho B 4 Viognier B 1 3,4 aBerry color: B, blanc (white); N, noir (black); R, rouge or rose (red or pink). bAppellation of Origin: Almansa (1), Jumilla (2), La Mancha (3), Manchuela (4), Méntrida (5), Mondéjar (6), Ribera del Júcar (7), Uclés (8), Valdepeñas (9). Payment wines: Dehesa del Carrizal (a), Dominio de Valdepusa (b), Finca Élez (c), Guijoso (d), Campo de La Guardia (e), Florentino(f), Casa del Blanco (g), Calzadilla (h). Supplemental Table 2 Primer concentrations for the four multiplex PCRs. Multiplex PCR D Multiplex PCR A Multiplex PCR B Multiplex PCR C Primer pair (dye)a Primer pair (dye)a Primer pair (dye)a Primer pair (dye)a VVS2 PET 0.15 µM VMC1b11 6-FAM 0.15 mM VMC4F3.1 NED 0.10 µM CCMP3 6-FAM 0.04 µM VVMD5 6-FAM 0.25 µM VVIB01 NED 0.10 mM VVIN16 PET 0.10 µM CCMP5 VIC 0.05 µM VVMD7 NED 0.10 µM VVIH54 VIC 0.10 mM VVIV37 VIC 0.10 µM CCMP10 NED 0.12 µM VrZAG62 VIC 0.05 µM VVIN73 VIC 0.10 mM VVIV67 PET 0.30 µM ccSSR9 PET 0.05 µM VrZAG79 PET 0.10 µM VVIP31 NED 0.15 mM VVMD5 6-FAM 0.35 µM ccSSR14 NED 0.10 µM VVMD27 6FAM 0.10 µM VVIP60 PET 0.17 mM VVMD21 PET 0.10 µM VrZAG67 VIC 0.10 µM VVIQ52 PET 0.05 mM VVMD27 6-FAM 0.10 µM VrZAG64 NED 0.10 µM VVMD24 PET 0.10 mM VVMD28 VIC 0.20 µM VrZAG83 PET 0.05 µM VVMD25 6-FAM 0.10 mM VVMD32 NED 0.08 µM VrZAG21 NED 0.10 µM VVMD7 NED 0.10 mM VVS2 PET 0.13 mM aOnly the forward primer is labeled with a dye. The concentrations of forward and reverse primer are identical. 1 Am. J. Enol. Vitic. Supplemental Data for: Fernández González, M., J. Martínez Gascueña, and A. Mena Morales. 2012. Identification and relationships of grapevine cultivars authorized for cultivation in Castilla La Mancha (Spain). Am. J. Enol. Vitic. 63:564-567. doi: 10.5344/ajev.2012.09010. Supplemental Table 3 Nuclear microsatellite genotypes and chlorotypes (C) for the cultivars studied. Allele sizes are expressed as base pairs. Cultivar VMC1b11 VVMD25 VVIH54 VVIN73 VVIP31 VVMD7 VVIB01 VVIQ52 VVS2 VVMD24 VVIP60 VVMD27 VVMD5 Airén 184 : 184 252 : 252 166 : 166 262 : 262 188 : 188 240 : 250 288 : 288 83 : 87 143 : 145 207 : 211 322 : 322 177 : 190 222 : 230 Albillo Dorado 172 : 184 238 : 252 164 : 166 254 : 260 178 : 190 236 : 250 288 : 292 83 : 87 143 : 145 207 : 209 318 : 322 179 : 190 224 : 232 Albillo Real 184 : 188 236 : 236 166 : 168 254 : 254 190 : 192 230 : 236 288 : 292 83 : 87 135 : 155 207 : 209 320 : 326 181 : 185 224 : 224 Bobal 184 : 188 238 : 264 166 : 168 262 : 262 174 : 184 236 : 240 288 : 292 81 : 83 145 : 147 207 : 209 326 : 326 177 : 185 224 : 230 Cabernet franc 184 : 188 236 : 252 166 : 180 262 : 266 188 : 188 236 : 260 288 : 288 81 : 81 139 : 147 207 : 207 314 : 324 177 : 185 222 : 236 Cabernet Sauvignon 184 : 184 236 : 246 166 : 180 262 : 266 188 : 188 236 : 236 288 : 288 81 : 87 139 : 151 207 : 216 305 : 314 171 : 185 228 : 236 Chardonnay 166 : 184 236 : 252 164 : 168 262 : 264 178 : 182 236 : 240 286 : 292 81 : 87 137 : 143 207 : 216 318 : 322 177 : 185 230 : 234 Coloraíllo 172 : 188 238 : 252 164 : 166 254 : 262 176 : 190 236 : 236 288 : 288 83 : 83 143 : 145 209 : 213 318 : 326 179 : 179 224 : 232 Coloraíllo/ G10 184 : 184 238 : 246 166 : 168 254 : 262 174 : 190 236 : 240 288 : 288 81 : 87 145 : 157 207 : 211 318 : 322 181 : 190 228 : 236 Coloraíllo/ G13 166 : 188 252 : 264 166 : 168 262 : 262 174 : 190 236 : 240 288 : 288 83 : 83 143 : 147 207 : 207 318 : 326 175 : 190 230 : 242 Forcallat Tinta 184 : 188 238 : 252 166 : 168 262 : 262 174 : 178 236 : 240 288 : 288 87 : 87 133 : 145 209 : 216 318 : 326 175 : 177 218 : 230 Garnacha Tinta 188 : 194 238 : 252 164 : 168 254 : 262 174 : 182 236 : 240 286 : 288 81 : 87 137 : 145 209 : 216 318 : 322 190 : 190 222 : 236 Garnacha Tintorera 182 : 188 238 : 238 164 : 168 254 : 262 174 : 182 236 : 240 288 : 292 81 : 87 133 : 145 207 : 209 318 : 322 177 : 190 222 : 234 Gewürtztraminer 170 : 172 246 : 246 164 : 164 262 : 262 178 : 192 240 : 254 286 : 292 81 : 87 151 : 151 211 : 216 318 : 320 185 : 185 228 : 234 Graciano 172 : 184 260 : 268 166 : 166 262 : 262 178 : 190 236 : 236 288 : 288 87 : 87 139 : 151 207 : 207 318 : 318 175 : 179 222 : 234 Macabeo/ Viura 184 : 184 236 : 238 166 : 166 262 : 262 174 : 194 236 : 236 288 : 292 83 : 87 133 : 145 207 : 209 318 : 326 185 : 190 230 : 232 Malbec 166 : 172 236 : 246 166 : 180 262 : 262 180 : 182 236 : 260 286 : 292 83 : 87 133 : 151 207 : 207 318 : 322 185 : 187 224 : 234 Malvar 184 : 188 238 : 252 164 : 168 260 : 262 174 : 188 236 : 240 288 : 288 81 : 83 143 : 145 207 : 213 318 : 326 175 : 190 232 : 236 Malvasía Aromática 166 : 184 238 : 252 158 : 164 256 : 262 172 : 182 240 : 246 288 : 292 87 : 87 143 : 145 207 : 216 318 : 330 175 : 179 222 : 222 Mazuela/ Cariñena 174 : 184 238 : 252 166 : 166 262 : 262 174 : 176 236 : 236 288 : 292 81 : 83 143 : 145 207 : 213 318 : 326 177 : 181 222 : 224 Mencía 172 : 184 238 : 246 164 : 166 262 : 262 178 : 190 246 : 254 292 : 305 83 : 87 145 : 151 207 : 207 318 : 322 177 : 185 222 : 232 Merlot 172 : 184 236 : 246 166 : 166 262 : 266 182 : 188 236 : 244 288 : 292 81 : 83 139 : 151 207 : 211 318 : 330 185 : 187 222 : 232 Merseguera 184 : 188 238 : 252 164 : 168 260 : 262 188 : 188 236 : 240 288 : 288 81 : 83 143 : 145 209 : 213 318 : 322 181 : 190 236 : 236 Monastrell 172 : 188 238 : 260 166 : 166 262 : 262 178 : 190 246 : 246 288 : 288 87 : 87 133 : 151 207 : 216 318 : 322 175 : 185 222 : 236 Montúa/ Chelva 184 : 188 252 : 252 166 : 166 262 : 262 174 : 178 240 : 250 288 : 288 87 : 87 143 : 151 207 : 207 318 : 326 177 : 181 230 : 234 Moravia Agria 166 : 184 252 : 252 166 : 168 262 : 262 188 : 188 236 : 250 288 : 292 83 : 83 145 : 151 207 : 207 318 : 326 175 : 175 224 : 232 Moravia Dulce/ G9 184 : 188 238 : 252 166 : 166 254 : 262 178 : 190 236 : 240 288 : 292 83 : 83 143 : 145 209 : 213 318 : 326 179 : 190 224 : 232 Moravia Dulce/ Crujidera 184 : 188 238 : 252 166 : 168 254 : 262 174 : 190 236 : 240 288 : 288 83 : 87 143 : 145 207 : 209 318 : 322 179 : 190 224 : 228 Muscat of Alexandria 166 : 184 246 : 246 166 : 166 262 : 262 186 : 190 246 : 248 288 : 292 81 : 81 133 : 149 211 : 211 318 : 322 175 : 190 224 : 228 Muscat à petits grains blancs 184 : 188 238 : 246 166 : 166 260 : 262 182 : 186 230 : 246 288 : 292 81 : 81 133 : 133 211 : 216 318 : 318 175 : 190 224 : 232 Pardillo/ Marisancho 184 : 188 238 : 246 166 : 168 262 : 262 174 : 190 236 : 246 288 : 305 81 : 87 145 : 157 211 : 213 318 : 322 181 : 190 232 : 236 Pardina/ Jaén Blanco 168 : 188 238 : 252 166 : 168 262 : 262 174 : 178 240 : 246 288 : 305 83 : 87 137 : 145 207 : 209 318 : 322 177 : 177 230 : 232 Parellada 188 : 188 236 : 238 164 : 166 262 : 262 174 : 188 240 : 246 288 : 288 83 : 87 133 : 143 207 : 209 318 : 326 175 : 177 224 : 230 Pedro Ximénez 168 : 188 238 : 246 166 : 166 262 : 262 174 : 186 236 : 236 288 : 305 87 : 87 133 : 145 207 : 211 318 : 322 177 : 181 232 : 236 Petit Verdot 168 : 172 246 : 252 164 : 180 262 : 264 178 : 192 236 : 260 288 : 292 83 : 83 143 : 155 213 : 213 318 : 322 175 : 185 222 : 228 Pinot noir 166 : 172 236 : 246 164 : 168 262 : 264 178 : 182 236 : 240 286 : 292 87 : 87 137 : 151 213 : 216 318 : 320 181 : 185 224 : 234 Prieto Picudo Tinto 166 : 170 246 : 252 164 : 168 262 : 262 178 : 188 236 : 254 286 : 292 81 : 87 143 : 151 207 : 211 318 : 322 175 : 185 222 : 234 Riesling 166 : 184 246 : 252 150 : 164 262 : 262 174 : 182 246 : 254 286 : 296 81 : 87 143 : 151 207 : 216 318 : 324 177 : 185 222 : 230 Rojal Tinta 184 : 188 238 : 252 166 : 166 254 : 262 182 : 190 236 : 240 288 : 288 83 : 83 137 : 145 207 : 209 318 : 318 181 : 190 224 : 230 Sauvignon blanc 172 : 184 238 : 246 164 : 166 262 : 262 188 : 192 236 : 254 286 : 288 81 : 87 133 : 151 216 : 216 318 : 320 171 : 185 224 : 228 Syrah 166 : 188 238 : 238 164 : 166 260 : 262 180 : 188 236 : 236 288 : 292 87 : 87 133 : 133 207 : 213 318 : 318 185 : 187 222 : 228 Tempranillo/ Cencibel 172 : 184 238 : 252 164 : 166 254 : 260 176 : 178 236 : 250 288 : 292 83 : 83 143 : 145 207 : 213 318 : 326 179 : 179 232 : 232 Tinto Velasco/ Frasco 172 : 184 236 : 236 164 : 166 260 : 262 182 : 188 230 : 250 288 : 288 87 : 87 133 : 133 207 : 213 318 : 322 175 : 181 228 : 234 Torrontés 184 : 188 238 : 252 166 : 168 260 : 262 174 : 188 236 : 236 288 : 288 83 : 87 143 : 145 209 : 216 318 : 322 181 : 190 230 : 232 Verdejo 166 : 170 246 : 246 164 : 164 260 : 262 174 : 178 236 : 254 286 : 288 81 : 87 151 : 157 211 : 216 318 : 322 179 : 185 222 : 234 Viognier 182 : 188 236 : 238 164 : 166 260 : 262 182 : 192 236 : 246 288 : 292 87 : 87 133 : 139 207 : 211 318 : 322 181 : 187 222 : 228 a Chlorotypes according to Supplemental Table 4.
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