1 1 The benefit-risk analysis of omega-3 polyunsaturated fatty acids and heavy metals in seven 2 smoked fish species from Siberia 3 4 Michail I. Gladyshev a,b,*,Olesia V. Anishchenkoa, Olesia N. Makhutovaa,b, Olesia V. 5 Kolmakovaa, Maria Y. Trusova M.Y.a,b, Vasiliy N. Morgunc, Iliada V. Gribovskayaa, Nadezhda 6 N. Sushchika,b 7 8 aInstitute of Biophysics of Siberian Branch of Russian Academy of Sciences, Akademgorodok, 9 50/50, Krasnoyarsk 660036, Russia 10 bSiberian Federal University, Svobodny av. 79, Krasnoyarsk, 660041, Russia 11 сFederal Regional Center for Standardization, Metrology and Testing in the Krasnoyarsk 12 Region, 1a Vavilov Str., Krasnoyarsk, 660093, Russia 13 14 * Corresponding author at:Institute of Biophysics of Siberian Branch of Russian Academy of 15 Sciences, Akademgorodok, 50/50, Krasnoyarsk 660036, Russia 16 E-mail address:
[email protected] (M.I.Gladyshev), telephone +7 902 920 8903 17 18 19 Abstract 20 21 In cold smoked species of genus Coregonus, identified by molecular genetic analysis, contents of 22 fatty acids and heavy metals and arsenic were measured. The highest content of sum of long- 23 chain polyunsaturated fatty acids of omega-3 family (LC-PUFA), namely eicosapentaenoic 24 (EPA) and docosahexaenoic (DHA) fatty acids, 6.53±0.78 mg g-1 wet weight, was characteristic 25 of tugun Coregonus tugun. This is the first quantitative estimation (mg LC-PUFA per g of 26 product) of the nutritive value of smoked fish. Thus, to obtain a daily personal doze of 2 27 EPA+DHA of 1 g, recommended for prevention of cardiovascular diseases, one needs to 28 consume 153 g of the smoked tugun.