DOCSLIB.ORG

Infundibulum As the 'Trichter' and the Caudal Half As the 'Tube'

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Infundibulum As the 'Trichter' and the Caudal Half As the 'Tube' Arch. histol. jap. Vol. 23, n. 5 (July 1963). P. 447-459. Dept. of Anim. Husb., Fac. of Fish. and Anim. Husb., Hiroshima Univ., Fukuyama, Japan. Histological and Histochemical Studies on the Oviduct of the Domestic Fowl with Special Reference to the Region of Uterovaginal Juncture. 鶏 の 卵 管, と く に 子 宮 と 腟 の 移 行 部 の 組 織 学 的 お よ び 組 織 化 学 的 研 究. Shunsaku FUJII 藤 井 俊 策. (Received May 30, 1963.) The histological structure of the oviduct of the domestic fowl has almost com- pletely been investigated by many workers, including SURFACE (1912), GIERSBERG (1921, 1922), FROBOSE (1928), BRADLEY (1928), and RICHARDSON (1935). SURFACE studied mainly the histological structure of the oviduct itself. RICHARD- SON observed in detail the function and histological s structure of the gland of the ovi- duct. At present, the oviduct is generally divided into five portions: the infundi- bulum or funnel, the magnum or albumen secreting portion, the isthmus, the uterus or shell-gland region, and the vagina. Each portion has its particular structure and physiological function for egg production. In addition, some workers have identified another portion as the region of juncture, where one type of mucosa intermingles with another type. For instance, GIERSBERG (1922) distinguished the cranial half of the infundibulum as the 'Trichter'and the caudal half as the 'Tube'. RICHARDSON, (1935)also subdivided the infundibulum into 'funnel'without glandsand the 'chalazi- ferous region'with glands, and insertedthe 'isthmo-uterineregion' between the isthmus and the uterus. In spite of such large number of histological observations as these, little has been reported on the histochemistry of the oviduct. In the course of histological and histochemical studies of the fowl oviduct, the author found the existence of characteristic mucous zones both at the caudal end of the uterus and the cranial end of the vagina. Each zone had definite histological and histo- chemical characteristics which distinguished it from its adjacent portion. The present paper deals mainly with morphological findings of the region of uterovaginal juncture. I. Materials and Methods. The oviducts collected from adult White Leghorn hens just after decapitation were used. The structure of the oviduct changes largely in accordance with the state of laying. Therefore, the oviducts used had been removed from nonlaying and laying hens in varying states of laying; that is, before ovulating, after laying, and stages where eggs were at different levels of the oviduct. They were classified according to the purpose of investigation and subjected to some suitable methods. For observation of general histological structure, they were fixed in BOUIN's and ZENKER's solution and made to paraffin sections 5-10μ thick, which were stained with hematoxylin 447 448 S. FUJII: and eosin and by HEIDENHAIN's azan and iron-hematoxylin methods. For demonst- ration of polysaccharide and mucopolysaccharide, they were fixed in CARNOY's solu- tion and 95% alcohol and stained by the periodic acid-SHIFF (PAS), LISON's alcian blue, and BEST's mucicarmine and mucihematein methods. For demonstration of the activities of alkaline and acid phosphatases, material was immersed in acetone, sectioned, and treated by GOMORI's calcium phosphate method and lead phosphate method, respectively. Some material was fixed in BAKER's calcium formalin and sectioned with a freezing microtome at a thickness of 15-20μ The sections were stained by the following techniques: for fat substances, BAKER's sudan black B and GIACCIO's sudan III stain, for phospholipids BAKER's acid hematein, MENSHIK's Nile blue and CIACCIO's sudan stain, for cholesterin and its ester SHULTZ's test, OKAMOTO's method and FEINGIN's digitonin method. In addition, the polarized- light method was employed for demonstration of cholesterin. II. Results. A. Macroscopical Observation. Macroscopically, the uterus is a somewhat flattened, elongated sac with trans- verse bands. The vagina is a narrow muscular tube, extending from the caudal end of the uterus to the cloaca. The boundary between the uterus and the vagina is indicated by the presence of a sphincter muscle. In more detail, the enlarged body of the uterus is constricted gradually in funnel shape as it approaches the narrow vaginal orifice, where the uterus ends abruptly. Caudal to the orifice, the vagina at first curves a little dorso-cranially, forming the first flexure. Then it ascends along the dorsal wall of the uterus for a short distance, about 1cm, enclosing the outer, longitudinal muscle of the uterus. It again turns suddenly backwards by forming the second flexure, after which it becomes smaller in caliber towards the cloaca, convoluting slightly in its course (Fig. 1). The inner surface of the uterus is thrown into a number of leaf-like folds of mucous membrane showing a faint rose pink color. On the other hand, that of the vagina is white in color and forms low, regular longitudinal folds. In the uterovaginal junc- ture, the transition of the mucous membrane is grad- ual, so that no clear demarcation is noticed, except a vague constriction around the vaginal orifice. Fig. 1. Outside appearance of the Careful observation of the terminal portion of the uterine and vaginal regions of the uterus indicates, however, that a narrow, ring- oviduct in a laying hen. Abbrevia- shaped zone, about 0.5-1cm wide, around the tion: Ub uterus proper, RU recessus vaginal orifice differs distinctly from the main por- uteri, Vg vaginal glanid region, Vp vagina proper. About 1/2. tion of the uterus in the color and shaps of the folds of mucous membrane. In this zone, the mucoss presents a characteristic grayish-white color, forming low, somewhat regularly ar- Histological and Histochemical Studies on the Oviduct etc. 449 ranged, longitudinal folds. These folds represent exactly an intermediate type between the uterine and the vaginal mucosal folds. This zone can be recognized by a naked eye, especially by the color of its mucosa, but the shade of its mucosa varies according to the activity of the oviduct. In general, this part of the uterus appears grayish- white in nonlaying hens, as well as in laying hens containing no egg in the uterus. Therefore, it is very characteristic of these hens. In hens which have just expelled eggs, this zone appears faint pink, with its regular color faded. Therefore, it is not so distinctly differentiated as before. The change in mucosal color suggests that some structural changes have occurred in the epithelium during the formation of egg-shell or expulsion of the egg. Accordingly, the caudal end of the uterus has not only these macroscopical features, but also such definite microscopical ones as mentioned below. So the author calls this portion as the recessus uteri, distinguishing it from the main portion of the uterus (hereinafter referred to as the uterus proper). Fig. 2. Inside appearance of the region of uterovaginal juncture. For abbreviations, see Fig. 1. Fig. 3. The mucosa of the uterus proper in a laying hen. Note luxuriant uterine glands. Hematoxylin and eosin (HE) staining. ×190. The beginning of the vagina, about 1cm long, between the first and the second flexure as mentioned above, is much larger in outside and inside diameter than the remainder of the vagina (hereinafter referred to as the vagina proper). The mucosal folds of the inner wall are lower and wider in the former than the latter. In addition, this part of the vagina is characterized histologically by the presence of many large tubular glands in the lamina propria which are to be mentioned below. So it is called the vaginal gland region, distinguishable from the vagina proper (Fig. 2). B. Histological Observation. As is generally known. the uterine mucosa is thrown into numerous folds with 450 S. PUJII: a few secondary ridges, which are lined by a pseudostratified epithelium composed of two kinds of cells, ciliated columnar and goblet cells. Situated in the lower position, goblet cells are extremely slender and scattered among tall ciliated cells located in the upper position. They are slightly positive to mucin stains in the PAS method, but are not stained with such dyes as alcian blue, mucicarmine, and mucihematein. The lamina propria is vascular and packed densely with highly branched tubular glands, known as the uterine glands. The glands are of large cuboidal cells with centrally located small, round nuclei (Fig. 3). Toward the caudal end of the uterus, the uterine glands decrease in number and gradually changes into small, simple tubular glands. Each gland has a narrow lumen Fig. 4. The mucosa of the recessus uteri in a laying hen. Note small tubular glands scattered in loosely arranged connective tissue. HE staining. ×190. Fig. 6. Fig. 6. The high-power magnification of Fig. 5. HE staining. ×400. Fig. 5. The mucosa of the juncture of the recessus uteri and the vaginal gland region. Note small glands mingling with large ones. HE staining. ×190. Fig. 5. Histological and Histochemical Studies on the Oviduct etc. 451 surrounded by a few low cuboidal cells. Although it seems to be different from the uterine gland, the cytoplasm of its glandular cells contains fine acidophilic granules characteristic of the uterine glands. In the recessus uteri continuous to the previous region, however, the surface of the fold is comparatively irregular, owing to an increase in secondary ridges. The lining epithelium has still essentially the same composition, although its goblet cells become smaller in number and larger in size than before and stainable with all the mucin stains employed before. It should be noted that the lamina propria of this region is composed of avascular loose connective tissue and has a few small glands scattered (Figs.
Load more

Recommended publications
  • Te2, Part Iii
    TERMINOLOGIA EMBRYOLOGICA Second Edition International Embryological Terminology FIPAT The Federative International Programme for Anatomical Terminology A programme of the International Federation of Associations of Anatomists (IFAA) TE2, PART III Contents Caput V: Organogenesis Chapter 5: Organogenesis (continued) Systema respiratorium Respiratory system Systema urinarium Urinary system Systemata genitalia Genital systems Coeloma Coelom Glandulae endocrinae Endocrine glands Systema cardiovasculare Cardiovascular system Systema lymphoideum Lymphoid system Bibliographic Reference Citation: FIPAT. Terminologia Embryologica. 2nd ed. FIPAT.library.dal.ca. Federative International Programme for Anatomical Terminology, February 2017 Published pending approval by the General Assembly at the next Congress of IFAA (2019) Creative Commons License: The publication of Terminologia Embryologica is under a Creative Commons Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) license The individual terms in this terminology are within the public domain. Statements about terms being part of this international standard terminology should use the above bibliographic reference to cite this terminology. The unaltered PDF files of this terminology may be freely copied and distributed by users. IFAA member societies are authorized to publish translations of this terminology. Authors of other works that might be considered derivative should write to the Chair of FIPAT for permission to publish a derivative work. Caput V: ORGANOGENESIS Chapter 5: ORGANOGENESIS
    [Show full text]
  • Sexual Reproduction & the Reproductive System Visual
    Biology 202: Sexual Reproduction & the Reproductive System 1) Label the diagram below. Some terms may be used more than once. Spermatozoa (N) Mitosis Spermatogonium (2N) Spermatids (N) Primary Oocyte (2N) Polar bodies (N) Ootid (N) Second polar body (N) Meiosis I Primary spermatocyte (2N) Oogonium (2N) Secondary oocyte (2N) Ovum (N) Secondary spermatocytes (2N) First polar body Meiosis II Source Lesson: Gametogenesis & Meiosis: Process & Differences 2) Label the diagram of the male reproductive system below. Seminal vesicle Testis Scrotum Pubic bone Penis Prostate gland Urethra Epididymis Vas deferens Bladder Source Lesson: Male Reproductive System: Structures, Functions & Regulation 3) Label the image below. Rectum Testis Ureter Bulbourethral gland Urethra Urinary bladder Pubic bone Penis Seminal vesicle Ductus deferens Epididymis Prostate gland Anus Source Lesson: Semen: Composition & Production 4) Label the structures below. Inner and outer lips of the vagina Mons pubis Vaginal opening Clitoris Anus Urethral opening Perineum Vulva Source Lesson: Female Reproductive System: Structures & Functions 5) Label the diagram below. Some terms may be used more than once. Clitoris Vulva Labia majora Labia minora Perineum Clitoral hood Vaginal opening Source Lesson: Female Reproductive System: Structures & Functions 6) Label the internal organs that make up the female reproductive system. Uterus Fallopian tubes Ovaries Cervix Vagina Endometrium Source Lesson: Female Reproductive System: Structures & Functions 7) Label the diagram below. LH Follicular
    [Show full text]
  • Vocabulario De Morfoloxía, Anatomía E Citoloxía Veterinaria
    Vocabulario de Morfoloxía, anatomía e citoloxía veterinaria (galego-español-inglés) Servizo de Normalización Lingüística Universidade de Santiago de Compostela COLECCIÓN VOCABULARIOS TEMÁTICOS N.º 4 SERVIZO DE NORMALIZACIÓN LINGÜÍSTICA Vocabulario de Morfoloxía, anatomía e citoloxía veterinaria (galego-español-inglés) 2008 UNIVERSIDADE DE SANTIAGO DE COMPOSTELA VOCABULARIO de morfoloxía, anatomía e citoloxía veterinaria : (galego-español- inglés) / coordinador Xusto A. Rodríguez Río, Servizo de Normalización Lingüística ; autores Matilde Lombardero Fernández ... [et al.]. – Santiago de Compostela : Universidade de Santiago de Compostela, Servizo de Publicacións e Intercambio Científico, 2008. – 369 p. ; 21 cm. – (Vocabularios temáticos ; 4). - D.L. C 2458-2008. – ISBN 978-84-9887-018-3 1.Medicina �������������������������������������������������������������������������veterinaria-Diccionarios�������������������������������������������������. 2.Galego (Lingua)-Glosarios, vocabularios, etc. políglotas. I.Lombardero Fernández, Matilde. II.Rodríguez Rio, Xusto A. coord. III. Universidade de Santiago de Compostela. Servizo de Normalización Lingüística, coord. IV.Universidade de Santiago de Compostela. Servizo de Publicacións e Intercambio Científico, ed. V.Serie. 591.4(038)=699=60=20 Coordinador Xusto A. Rodríguez Río (Área de Terminoloxía. Servizo de Normalización Lingüística. Universidade de Santiago de Compostela) Autoras/res Matilde Lombardero Fernández (doutora en Veterinaria e profesora do Departamento de Anatomía e Produción Animal.
    [Show full text]
  • Uterus – Dilation
    Uterus – Dilation Figure Legend: Figure 1 Uterus - Dilation of the uterine lumen in a female B6C3F1/N mouse from a chronic study. There is dilation of the uterine horn. Figure 2 Uterus - Dilation in a female B6C3F1/N mouse from a chronic study (higher magnification of Figure 1). The endometrial epithelium is cuboidal. Figure 3 Uterus - Dilation in a female B6C3F1/N mouse from a chronic study. There is dilation of the uterine lumen, which contains flocculent, eosinophilic material. Figure 4 Uterus - Dilation in a female B6C3F1/N mouse from a chronic study (higher magnification of Figure 3). There is flattened epithelium and eosinophilic material in the uterine lumen. Comment: Dilation of uterine horns (Figure 1, Figure 2, Figure 3, and Figure 4) is commonly observed at necropsy, and frequently these uteri have accumulations of excessive amounts of fluid within the 1 Uterus – Dilation lumen. Uterine dilation is relatively commonly seen in both rats and mice and may be segmental. Luminal dilation may be associated with stromal polyps or occur secondarily to hormonal imbalances from ovarian cysts or to a prolonged estrus state after cessation of the estrus cycle in aged rodents. Administration of progestins, estrogens, and tamoxifen in rats has been associated with uterine dilation. Luminal dilation is normally observed at proestrus and estrus in cycling rodents and should not be diagnosed. Increased serous fluid production is part of the proestrus phase of the cycle judged by the vaginal epithelium (which shows early keratinization covered by a layer of mucified cells) and should not be diagnosed. With uterine dilation, the endometrial lining is usually attenuated or atrophic and the wall of the uterus thinned due to the increasing pressure, but in less severe cases the endometrium can be normal (Figure 2).
    [Show full text]
  • The Functions of Uterine Secretions R
    Printed in Great Britain J. Reprod. Fert. (1988) 82,875-892 @ 1988 Journals of Reproduction & Fertility Ltd The functions of uterine secretions R. M. Roberts and F. W. Bazer Departments of Biochemistry and Animal Sciences, University of Missouri, Columbia, MO 65211, U.S.A.; and Department of Animal Science, University of Florida, Gainesville, FL 32611, U.S.A. Summary. The likely functions of uterine secretions, often termed histotroph, in the nurture of the early conceptus are reviewed. Particular emphasis has been placed on the pig in which the uterus synthesizes and secretes large amounts of protein in response to progesterone. In this species, which possesses a non-invasive, diffuse type of epithelio- chorial placentation, the secretions provide a sustained embryotrophic environment which is distinct from that of serum. A group of basic proteins dominates these uterine secretions after Day 1 1 of pregnancy and its best characterized member is uteroferrin, an iron-containing acid phosphatase with a deep purple colour. Evidence has accumulated to suggest that uteroferrin, rather than functioning as an acid phosphatase, is involved in transporting iron to the conceptus. Three basic polypeptides which are found non- covalently associated with uteroferrin have been shown to be antigenically closely related to one another and to have arisen by post-translational processing from a common precursor molecule. Their function is unknown. A group of basic protease inhibitors has been identified which bear considerable sequence homology to bovine pancreatic trypsin inhibitor (aprotinin) and may control intrauterine proteolytic events initiated by the conceptuses. The last basic protein so far characterized is lysozyme which is presumed to have an antibacterial role.
    [Show full text]
  • Nomina Histologica Veterinaria, First Edition
    NOMINA HISTOLOGICA VETERINARIA Submitted by the International Committee on Veterinary Histological Nomenclature (ICVHN) to the World Association of Veterinary Anatomists Published on the website of the World Association of Veterinary Anatomists www.wava-amav.org 2017 CONTENTS Introduction i Principles of term construction in N.H.V. iii Cytologia – Cytology 1 Textus epithelialis – Epithelial tissue 10 Textus connectivus – Connective tissue 13 Sanguis et Lympha – Blood and Lymph 17 Textus muscularis – Muscle tissue 19 Textus nervosus – Nerve tissue 20 Splanchnologia – Viscera 23 Systema digestorium – Digestive system 24 Systema respiratorium – Respiratory system 32 Systema urinarium – Urinary system 35 Organa genitalia masculina – Male genital system 38 Organa genitalia feminina – Female genital system 42 Systema endocrinum – Endocrine system 45 Systema cardiovasculare et lymphaticum [Angiologia] – Cardiovascular and lymphatic system 47 Systema nervosum – Nervous system 52 Receptores sensorii et Organa sensuum – Sensory receptors and Sense organs 58 Integumentum – Integument 64 INTRODUCTION The preparations leading to the publication of the present first edition of the Nomina Histologica Veterinaria has a long history spanning more than 50 years. Under the auspices of the World Association of Veterinary Anatomists (W.A.V.A.), the International Committee on Veterinary Anatomical Nomenclature (I.C.V.A.N.) appointed in Giessen, 1965, a Subcommittee on Histology and Embryology which started a working relation with the Subcommittee on Histology of the former International Anatomical Nomenclature Committee. In Mexico City, 1971, this Subcommittee presented a document entitled Nomina Histologica Veterinaria: A Working Draft as a basis for the continued work of the newly-appointed Subcommittee on Histological Nomenclature. This resulted in the editing of the Nomina Histologica Veterinaria: A Working Draft II (Toulouse, 1974), followed by preparations for publication of a Nomina Histologica Veterinaria.
    [Show full text]
  • Índice De Denominacións Españolas
    VOCABULARIO Índice de denominacións españolas 255 VOCABULARIO 256 VOCABULARIO agente tensioactivo pulmonar, 2441 A agranulocito, 32 abaxial, 3 agujero aórtico, 1317 abertura pupilar, 6 agujero de la vena cava, 1178 abierto de atrás, 4 agujero dental inferior, 1179 abierto de delante, 5 agujero magno, 1182 ablación, 1717 agujero mandibular, 1179 abomaso, 7 agujero mentoniano, 1180 acetábulo, 10 agujero obturado, 1181 ácido biliar, 11 agujero occipital, 1182 ácido desoxirribonucleico, 12 agujero oval, 1183 ácido desoxirribonucleico agujero sacro, 1184 nucleosómico, 28 agujero vertebral, 1185 ácido nucleico, 13 aire, 1560 ácido ribonucleico, 14 ala, 1 ácido ribonucleico mensajero, 167 ala de la nariz, 2 ácido ribonucleico ribosómico, 168 alantoamnios, 33 acino hepático, 15 alantoides, 34 acorne, 16 albardado, 35 acostarse, 850 albugínea, 2574 acromático, 17 aldosterona, 36 acromatina, 18 almohadilla, 38 acromion, 19 almohadilla carpiana, 39 acrosoma, 20 almohadilla córnea, 40 ACTH, 1335 almohadilla dental, 41 actina, 21 almohadilla dentaria, 41 actina F, 22 almohadilla digital, 42 actina G, 23 almohadilla metacarpiana, 43 actitud, 24 almohadilla metatarsiana, 44 acueducto cerebral, 25 almohadilla tarsiana, 45 acueducto de Silvio, 25 alocórtex, 46 acueducto mesencefálico, 25 alto de cola, 2260 adamantoblasto, 59 altura a la punta de la espalda, 56 adenohipófisis, 26 altura anterior de la espalda, 56 ADH, 1336 altura del esternón, 47 adipocito, 27 altura del pecho, 48 ADN, 12 altura del tórax, 48 ADN nucleosómico, 28 alunarado, 49 ADNn, 28
    [Show full text]
  • Seasonal Variation of the Oviduct of the American Alligator, Alligator Mississippiensis (Reptilia: Crocodylia)
    JOURNAL OF MORPHOLOGY 270:702–713 (2009) Seasonal Variation of the Oviduct of the American Alligator, Alligator mississippiensis (Reptilia: Crocodylia) April Bagwill,1* David M. Sever,2 and Ruth M. Elsey3 1Department of Zoology, Oklahoma State University, Stillwater, Oklahoma 74078 2Department of Biological Sciences, Southeastern Louisiana University, Hammond, Louisiana 70402 3Louisiana Department of Wildlife and Fisheries, Rockefeller Wildlife Refuge, Grand Chenier, Louisiana 70643 ABSTRACT The annual oviductal cycle of the American The range of the American alligator spans the alligator, Alligator mississippiensis, is described using light southeastern coastal plain from central Texas east and electron microscopy. Previous work done by Palmer to North Carolina, with small populations reaching and Guillette ([1992] Biol Reprod 46:39–47) shed some the southeast corner of Oklahoma and the south- light on the reproductive morphology of the female alliga- ern third of Arkansas in the most northern part of tor oviduct; however, their study was limited and did not report details relating to variation across the reproductive their range (Conant and Collins, 1998). Alligators season. We recognize six variable regions of the oviduct: in- were close to extinction during the 1970s due to fundibulum, tube, isthmus, anterior uterus, posterior overhunting; however, effective management plans uterus, and vagina. Each area shows variation, to some were put into place and now the population is well degree, in the histochemistry and ultrastructure of oviduc- over several million. Louisiana has the highest tal secretions. Peak secretory activity occurs during the population at around 1.5 million wild alligators months of May and June, with the greatest variation occur- (Louisiana Department of Wildlife and Fisheries, ring in the tube and anterior uterus.
    [Show full text]
  • Histomorphological Changes in the Tubular Genitalia of the Sow (Sus Scrofa Domesticus) As Influenced by Age Harpal Singh Bal Iowa State University
    Iowa State University Capstones, Theses and Retrospective Theses and Dissertations Dissertations 1969 Histomorphological changes in the tubular genitalia of the sow (Sus scrofa domesticus) as influenced by age Harpal Singh Bal Iowa State University Follow this and additional works at: https://lib.dr.iastate.edu/rtd Part of the Animal Structures Commons, and the Veterinary Anatomy Commons Recommended Citation Bal, Harpal Singh, "Histomorphological changes in the tubular genitalia of the sow (Sus scrofa domesticus) as influenced by age" (1969). Retrospective Theses and Dissertations. 4639. https://lib.dr.iastate.edu/rtd/4639 This Dissertation is brought to you for free and open access by the Iowa State University Capstones, Theses and Dissertations at Iowa State University Digital Repository. It has been accepted for inclusion in Retrospective Theses and Dissertations by an authorized administrator of Iowa State University Digital Repository. For more information, please contact [email protected]. This dissertation has been microiihned exactly as received 69-15,597 BAL, Harpal Singh, 1928- HISTOMORPHOLOGICAL CHANGES IN THE TUBULAR GENITALIA OF THE SOW (SUS SCROFA DOMESTICUS) AS INFLUENCED BY AGE. Iowa State University, Ph.D., 1969 Anatomy University Microfilms, Inc., Ann Arbor, Michigan HISTOMORPHOLOGICAL CHANGES IN THE TUBULAR GENITALIA OF THE SOW (SUS SCROFA DOMESTICUS) AS INFLUENCED BY AGE Earpal Singh Bal A Dissertation Submitted to the Graduate Faculty in Partial Fulfillment of The Requirements for the Degree of DOCTOR OF PHILOSOPHY
    [Show full text]
  • Downloaded from Bioscientifica.Com at 09/26/2021 05:28:00AM Via Free Access 416 A
    LOCALIZATION OF TISSUE-SPECIFIC ANTIGENS IN THE RABBIT OVARY, OVIDUCT AND UTERUS BY THE FLUORESCENT ANTIBODY TECHNIQUE A. G. SACCO and C. A. SHIVERS Department of ^oology, University of Tennessee, Knoxville, Tennessee 37916, U.S.A. {Received 17th January 1972) Summary. The indirect fluorescent antibody technique was used to localize the reproductive tissue-specific antigens present in the rabbit ovary, oviduct and uterus. The two ovarian antigens were localized in the zona pellucida, theca interna and atretic follicles; the six oviducal antigens in the epithelium of the mucosa; and the two uterine antigens in the endometrial stroma and lumina of uterine glands and the lamina propria of the cervix. INTRODUCTION Ouchterlony analysis indicates that the ovaries and reproductive tract of the female rabbit contain reproductive tissue-specific antigens (Sacco & Shivers, 1973b). A minimum of two such antigens was detected in the ovary, six in the oviduct and two in the uterus. Since these antigens may be of importance in reproductive processes, it seemed of interest to determine their location within the respective tissues. It was the purpose of this investigation to localize these antigens using the indirect fluorescent antibody technique. MATERIALS AND METHODS Preparation of antisera Sheep antisera produced against rabbit ovary, oviduct and uterus were pre¬ pared and absorbed according to procedures described by Sacco & Shivers (1973b). The antisera were rendered specific to the reproductive tissues by absorption with rabbit plasma, kidney and spleen. Antiserum to be labelled with fluorescein isothiocyanate (FITC) for the indirect fluorescent antibody technique (Goldman, 1968) was produced in rabbits against the y-globulin fraction (ammonium sulphate precipitation) of normal sheep serum.
    [Show full text]
  • Ansc 630: Reproductive Biology 1
    ANSC 630: REPRODUCTIVE BIOLOGY 1 INSTRUCTOR: FULLER W. BAZER, PH.D. OFFICE: 442D KLEBERG CENTER EMAIL: [email protected] OFFICE PHONE: 979-862-2659 ANSC 630: INFORMATION CARD • NAME • MAJOR • ADVISOR • RESEARCH INTERESTS • PREVIOUS COURSES: – Reproductive Biology – Biochemistry – Physiology – Histology – Embryology OVERVIEW OF FUNCTIONAL REPRODUCTIVE ANATOMY: THE MAJOR COMPONENTS PARS NERVOSA PARS DISTALIS Hypothalamic Neurons Hypothalamic Neurons Melanocyte Supraoptic Stimulating Hormone Releasing Paraventricular Factor Axons Nerve Tracts POSTERIOR PITUITARY INTERMEDIATE LOBE OF (PARS NERVOSA) Oxytocin - Neurophysin PITUITARY Vasopressin-Neurophysin Melanocyte Stimulating Hormone (MSH) Hypothalamic Divisions Yen 2004; Reprod Endocrinol 3-73 Hormone Profile of the Estrous Cycle in the Ewe 100 30 30 50 15 15 GnRH (pg/ml)GnRH GnRH (pg/ml)GnRH 0 0 (pg/ml)GnRH 0 4 h 4 h 4 h PGF2α Concentration 0 5 10 16 0 Days LH FSH Estradiol Progesterone Development of the Hypophysis Dubois 1993 Reprod Mamm Man 17-50 Neurons • Cell body (soma; perikaryon) – Synthesis of neuropeptides • Cellular processes • Dendrites • Axon - Transport • Terminals – Storage and Secretion Yen 2004 Reprod Endocrinol 3-73 • Peptide neurotransmitter synthesis • Transcription – Gene transcribes mRNA • Translation – mRNA translated for protein synthesis • Maturation – post-translational processing • Storage in vesicles - Hormone secreted from vesicles Hypothalamus • Mid-central base of brain – Optic chiasma – 3rd ventricle – Mammillary body • Nuclei – Clusters of neurons • Different
    [Show full text]
  • And Uterine Glands on Decidualization and Fetoplacental Development
    Sexually dimorphic effects of forkhead box a2 (FOXA2) and uterine glands on decidualization and fetoplacental development Pramod Dhakala, Andrew M. Kellehera, Susanta K. Behuraa,b, and Thomas E. Spencera,c,1 aDivision of Animal Sciences, University of Missouri, Columbia, MO 65211; bInstitute for Data Science and Informatics, University of Missouri, Columbia, MO 65211; and cDepartment of Obstetrics, Gynecology, and Women’s Health, University of Missouri, Columbia, MO 65201 Contributed by Thomas E. Spencer, August 11, 2020 (sent for review July 7, 2020; reviewed by Ramakrishna Kommagani and Geetu Tuteja) Glands of the uterus are essential for pregnancy establishment. the stroma/decidua (15), and recent evidence indicates that Forkhead box A2 (FOXA2) is expressed specifically in the glands of uterine glands influence stromal cell decidualization (15–18). the uterus and a critical regulator of glandular epithelium (GE) Additionally, uterine glands have direct connections to the de- differentiation, development, and function. Mice with a condi- veloping placenta in mice (19, 20) and humans (21). tional deletion of FOXA2 in the adult uterus, created using the Mice lacking leukemia inhibitory factor (LIF) and uterine lactotransferrin iCre (Ltf-iCre) model, have a morphologically gland knockout mice and sheep are infertile, thereby establishing normal uterus with glands, but lack FOXA2-dependent GE- the importance of the uterine glands, their secretions, and expressed genes, such as leukemia inhibitory factor (LIF). Adult products for embryo implantation and pregnancy (22–28). iCre/+ f/f FOXA2 conditional knockout (cKO; Ltf Foxa2 ) mice are infer- Forkhead box (FOX) transcription factors play essential roles in tile due to defective embryo implantation arising from a lack of cell growth, proliferation, and differentiation in a number of LIF, a critical implantation factor of uterine gland origin.
    [Show full text]