HTTPS://JOURNALS.KU.EDU/REPTILESANDAMPHIBIANSTABLE OF CONTENTS IRCF REPTILES & AMPHIBIANSREPTILES • VOL &15, AMPHIBIANS NO 4 • DEC 2008 • 27(3):472–473189 • DEC 2020

IRCF REPTILES & AMPHIBIANS CONSERVATION AND NATURAL HISTORY

TABLE OF CONTENTS

FirstFEATURE Record ARTICLES of the Naga Hills Horned Frog, . Chasing Bullsnakes (Pituophis catenifer sayi) in Wisconsin: MegophrysOn the Road to Understanding awuh the Ecology and Mahony,Conservation of the Midwest’s Giant Kamei, Serpent ...... Joshua Teeling, M. Kapfer 190 . The Shared History of Treeboas (Corallus grenadensis) and Humans on Grenada: A andHypothetical Excursion Biju ...... (Anura: Megophryidae:Robert W. Henderson 198 RESEARCH ARTICLES Megophryinae),. The Texas Horned Lizard in Central and Western Texas ...... from Emily Henry,Mizoram, Jason Brewer, Krista Mougey, and IndiaGad Perry 204 . The Knight Anole (Anolis equestris) in Florida ...... Brian J. Camposano, Kenneth L. Krysko, Kevin M. Enge, Ellen M. Donlan, and Michael Granatosky 212 Samuel Lalronunga1, Vanramliana2, Isaac Zosangliana1, K. Lalhmangaiha1, Esther Lalhmingliani1 CONSERVATION ALERT 1Systematics and Toxicology Laboratory, Department of Zoology, Mizoram University, Aizawl 796004, Mizoram, India ([email protected]) . World’s Mammals2Department in Crisis ...... of Zoology, Pachhunga University College, Aizawl 796001, Mizoram,...... India 220 . More Than Mammals ...... 223 . The “Dow Jones Index” of Biodiversity ...... 225

HUSBANDRY he Naga Hills. HornedCaptive Care Frog of the Central (Megophrys Netted Dragon awuh ...... ) has been Shannon Plummer 226 Tknown only from the vicinity of Kohima and Star Lakes in southern and PROFILEeastern Nagaland, but was expected to have a . more extensive geographicalKraig Adler: Adistribution Lifetime Promoting due Herpetology to its occurrence ...... Michael L. Treglia 234 at a wide elevationalCOMMENTARY range (1,650–2,220 m asl) (Mahony et al. 2020). Herein we. The provide Turtles Have the Been first Watching record Me of ...... the Naga Hills Eric Gangloff 238

Horned Frog (Fig.BOOK 1) from REVIEW Mizoram, the first record of the species outside Nagaland.. Threatened The Amphibians new record of the World extends edited by the S.N. range Stuart, M. Hoffmann, J.S. Chanson, N.A. Cox, of this species about 300R. Berridge, km southwestP. Ramani, and ofB.E. the Young closest ...... known Robert Powell 243 locality in Nagaland and also extends the elevational range of this species down toCONSERVATION 1,478 m asl. RESEARCH REPORTS: Summaries of Published Conservation Research Reports ...... 245  NATURAL HISTORY RESEARCH REPORTS: Summaries of Published Reports on Natural History ...... 247 During a herpetofaunal NEWBRIEFS ...... survey on 16 June 2017, we ...... 248 collected frogs ofEDITORIAL the genus INFORMATION Megophrys in ...... the Hmuifang ...... 251  Community ForestFOCUS Reserve ON CONSERVATION at Hmuifang: AVillage, Project You Aizawl Can Support ...... 252 District, Mizoram (23º27.213'N, 92º45.181'E; elev. 1,478 m asl; Fig. 2). Prior to fixation of specimens, we excised muscle tissue from the right thigh and stored it in absolute ethanol for DNA extraction. SpecimensFront Cover. were Shannon deposited Plummer. in the Back Cover. Michael Kern Totat et velleseque audant mo Totat et velleseque audant mo estibus inveliquo velique rerchil estibus inveliquo velique rerchil erspienimus, quos accullabo. Ilibus erspienimus, quos accullabo. Ilibus aut dolor apicto invere pe dolum aut dolor apicto invere pe dolum fugiatis maionsequat eumque fugiatis maionsequat eumque moditia erere nonsedis ma sectiatur moditia erere nonsedis ma sectia- ma derrovitae voluptam, as quos Fig.tur 2.ma Map derrovitae showing voluptam, the asdistribution of the Naga Hills Horned Frog accullabo. (Megophrys awuh). The orange star indicates the type locality, the orange circle a previous record, and the red circle the new record. Symbols may represent more than one proximate site.

herpetological collection of the Systematics and Toxicology Laboratory, Department of Zoology, Mizoram University, India (MZUHC 305–7, MZUHC 313–4). Fragments of 16S rRNA mitochondrial gene sequences were generated following Lalronunga and Lalrinchhana Fig. 1. A female Naga Hills Horned Frog (Megophrys awuh) from Hmuifang, Mizoram, India (MZUHC 307). Photograph by Samuel (2017) for comparison with members of the Megophrys mega- Lalronunga. cephala species group available in GenBank. Sequences were

Copyright is held by the authors. Articles in R&A are made available under a 472 Reptiles & Amphibians ISSN 1098-6324 Creative Commons Attribution-NonCommercial 4.0 International license. LALRONUNGA ET AL. REPTILES & AMPHIBIANS • 27(3):472–473 • DEC 2020

Table 1. Uncorrected p-distances between species of the Megophrys (Xenophrys) megacephala species group (16S rRNA). GenBank accession numbers are listed in parentheses after the name of the species.

M. ancrae (KY022318) M. awuh (KY022319) 0.105 M. awuh (MT793042–6) 0.094 0.022 M. dzukou (KY022324) 0.079 0.121 0.119 M. megacephala (KY022315) 0.200 0.220 0.220 0.237 M. oropedion (KY022316) 0.091 0.111 0.106 0.119 0.197 M. numhbumaeng (MN734393) 0.091 0.111 0.106 0.119 0.197 0.000 M. oropedion (KY022317) 0.088 0.120 0.113 0.117 0.202 0.047 0.047 M. serchhipii (KY022323) 0.095 0.094 0.099 0.123 0.209 0.105 0.105 0.108 M. zunhebotoensis (KY022322) 0.093 0.090 0.087 0.115 0.218 0.099 0.099 0.112 0.098

aligned using ClustalW integrated in MEGA 7 (Molecular Directorate of Science and Technology, Government of Evolutionary Genetics Analysis) software (Kumar et al. 2016) Mizoram for financial support of field collection (No. B. with the default settings. Uncorrected pairwise genetic dis- 13012/1/2017-DST). VRL and SL thank the Department tances (p-distance) between sequences (Table 1) were deter- of Biotechnology, New Delhi, for financial support to the mined with MEGA 7 (Kumar et al. 2016). The genetic dis- Institutional Advanced-Level Biotech Hub (BT/22/NE/2011, tance between the sequences from Hmuifang, Mizoram (n 19 July 2017), which supported the molecular work in this = 5, Genbank accession numbers MT793042–6), and the study. We also thank the local authorities of Hmuifang paratype of M. awuh (accession number KY022319) is only Village for allowing us to conduct a survey in their area. 0.022. Lalengliana et al. (2016) had previously misidentified frogs of this species as Megophrys serchhipii, a species described Literature Cited by Mathew and Sen (2009) from Serchhip, Mizoram, about Kumar, S., G. Stecher, and K. Tamura. 2016. MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for bigger datasets. Molecular Biology and 18 km airline distance from the present study site. Evolution 33: 1870–1874. Lalengliana, H.C., Vanlalhmangaihsangi, and H.T. Lalremsanga. 2016. Survey on Acknowledgements anuran fauna of Hmuifang Community Reserved Forest with new reports from Mizoram, pp. 9–16. In: Proceedings Sub-Committee, Mizoram Science We thank the Principal, Pachhunga University College and Congress 2016 (eds.), Science and Technology for Shaping the Future of the Head, Department of Zoology, Mizoram University Mizoram. Proceedings of the Mizoram Science Congress 2016. Allied Publishers for Institutional support; Lalnunhlua, Lalkhawngaiha Sailo, Pvt. Ltd., New Dehli, India. Lalronunga S., and C. Lalrinchhana 2017. Morphological and molecular charac- Malsawmdawngliana, and Vanlalchhuanga for their assis- terization of Theloderma moloch (Anura: Rhacophoridae) from Indo-Burma tance during fieldwork; and the Chief Wildlife Warden, biodiversity hotspot of northeast India. Science Vision 17: 148–159. Environment, Forest and Climate Change Department, Mahony S., R.G. Kamei, E.C. Teeling, and S.D. Biju. 2020. Taxonomic review of the Asian Horned Frogs (Amphibia: Megophrys Kuhl & Van Hasselt) of Northeast Government of Mizoram, India, for issuing a research per- India and Bangladesh previously misidentified as M. parva (Boulenger), with mit (A.38011/5/2011-CWLW/338). SL and EL thank the descriptions of three new species. Journal of Natural History 54: 119–194.

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