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Program & Abstract Book PROGRAM & ABSTRACT BOOK Content Scientific Program Committee 1 Local Organising Committee 1 Program at Glance 2 Detailed Program 4 Zebrafish 2020 Chairs 17 Zebrafish 2020 Speakers 18 List of Oral Abstracts 21 List of Poster Abstracts 71 List of Presenters 198 List of Oral Presentation Co-Authors 199 List of Poster Co-Authors 203 Zebrafish 2020 Sponsors 211 Scientific Program Committee Petr Bartunek, Institute of Molecular Genetics, Prague, Czech Republic Michael Brand, Center for Regenerative Therapies Dresden TU, Dresden, Germany Elisabeth Busch-Nentwich, University of Cambridge, UK David Langenau, Massachusetts General Hospital, Harvard Medical School, Boston, USA Chris Lawrence, Boston Children’s Hospital, Boston, USA Graham Lieschke, Australian Regenerative Medicine Institute, Melbourne, Australia Christian Mosimann, University of Denver, USA Trista North, Harvard Medical School, Boston, USA Liz Patton, MRC Human Genetics Unit, University of Edinburgh, UK Ewa Snaar-Jagalska, Leiden University, Leiden, Netherlands Tatjana Sauka-Spengler, Oxford University, Oxford, UK Stefan Schulte-Merker, Münster University, Münster, Germany Didier Stainier, Max Planck Institute for Heart and Lung Research, Bad Nauheim, Germany Leonard Zon, Harvard University, Boston Children’s Hospital, HHMI, Boston, USA Randy Peterson, University of Utah, USA Francisco Pelegri, MAdison University, USA Bettina Schmid, LMU Munich, Germany Massimo Santoro, University of Padua, Italy Herwig Baier, MPI Munich, Germany Local Organising Committee Petr Bartunek, Institute of Molecular Genetics, Prague, Czech Republic Jana Dvorakova, C-IN, Professional Congress Organiser, Czech Republic Jana Oltova, Institute of Molecular Genetics, Prague, Czech Republic Sarka Simova, Institute of Molecular Genetics, Prague, Czech Republic 1 Session Schedule Program at GlanceAll session times are in Central European Time. Open session and see the session info to find out when it starts in your time zone. The organizer reserves the right to make any program changes and to adjust the presentations’ length. th Monday,Monday, October October 26th Tuesday, 26 October 27th ​ Virtual Room A Virtual Room B 0800 ​ Welcome Address ​ Epigenetics, Gene Regulation and Genomic 0900 Resources ​ ​ Coffee Break + Virtual Exhibition 1000 Morphogenesis and Organogenesis I ​ ​ Diamond Sponsor / The Tecniplast InterZebTEC: a 1100 Novel Technical Approach to the Environmental Microbiological Monitoring of Zebrafish ​ Coffee Break + Virtual Exhibition Coffee Break + Virtual Exhibition ​ Germ Line, Early Development and Patterning I Neurobiology I 1200 ​ ​ Lunch Break + Sponsor Pitches 1300 ​ Keynote Session - Prof. Dr. Nikolaus Rajewsky ​ 1400 ​ Emerging Technologies ​ 1500 ​ ​ Community Session 1600 ​ ​ Coffee Break + Virtual Exhibition 00 17 Disease Models I Husbandry and Aquaculture ​ ​ 1800 Coffee Break + Virtual Exhibition Coffee Break + Virtual Exhibition ​ Regeneration I Cancer ​ 1900 ​ 2 ​ Session Schedule All session times are in Central European Time. Open session and see the session info to find out when it starts in your time zone. The organizer reserves the right to make any program changes and to adjust the presentations’ length. th Tuesday,Monday, October October 26th Tuesday, 27 October 27th ​ Virtual Room A Virtual Room B 0800 ​ Germ Line, Early Development and Patterning II Immunity and Infection ​ 0900 ​ ​ Coffee Break + Virtual Exhibition Coffee Break + Virtual Exhibition Regeneration II Cell Signalling and Metabolism 1000 ​ ​ 00 11 Coffee Break + Virtual Exhibition Coffee Break + Virtual Exhibition ​ Neurobiology II Chemical Biology and Drug Discovery ​ 1200 ​ Lunch Break + Sponsor Pitches Lunch Break + Sponsor Pitches ​ 1300 Morphogenesis and Organogenesis II Circuits and Behavior ​ ​ 1400 Coffee Break + Virtual Exhibition Coffee Break + Virtual Exhibition ​ ​ Disease Models II Gold Sponsor - Aquaneering / Aquatic Facility Design: Considerations for Aquatic Facility Planning 1500 ​ Gold Sponsor - Plexx / Iwaki Aquatic Workshop on the New LAbREED™ Zebrafish Housing System ​ Coffee Break + Virtual Exhibition Coffee Break + Virtual Exhibition 00 16 Stem Cells Toxicology ​ ​ 1700 ​ Keynote Session - Prof. Lalita Ramakrishnan ​ 1800 ​ Closing Remarks ​ 1900 ​ ​ 3 Detailed Program Monday October 26, 2020 Welcome Address 08:30–08:50 VIRTUAL ROOM A Petr Bartunek (Czech Republic) Scientific Session: Epigenetics, Gene Regulation and Genomic Resources 08:50–09:55 VIRTUAL ROOM A Chairs: Tatjana Sauka-Spengler (United Kingdom) Elisabeth Busch-Nentwich (United Kingdom) 08:50 Cis-regulatory similarities in the zebrafish and human pancreas uncover potential disease-related enhancers Jose Bessa (Portugal) 09:05 Single-cell-resolved dynamics of chromatin architecture delineate cell and regulatory states in wildtype and cloche/npas4l mutant zebrafish embryos Scott Lacadie (Germany) 09:17 The complexity within you: Single-cell analysis revealed hidden heterogeneity in the thyroid gland Pierre Gillotay (Belgium) 09:29 A novel transgenic zebrafish line to study epigenetics during disease and development Aniket Gore (USA) Scientific Session: Morphogenesis and Organogenesis I 10:15–11:20 VIRTUAL ROOM A Chair: Didier Stainier (Germany) 10:15 Planar Cell Polarity signaling couples nuclear mechanics with changes in muscle differentiation Anne M. Merks (Germany) 10:30 Laminins regulate cardiac growth through restricting second heart field addition Emily Noël (United Kingdom) 4 10:42 Loss of Smad4 steers embryonic development towards a BMP zero morphotype Luca Guglielmi (United Kingdom) 10:54 The dead box helicase Ddx21 controls cell cycle progression and cell number expansion during embryonic lymphangiogenesis Kaska Koltowska (Sweden) Sponsor Session: Diamond Sponsor / The Tecniplast InterZebTEC: a Novel Technical Approach to the Environmental Microbiological Monitoring of Zebrafish 10:50–11:20 VIRTUAL ROOM B Tecniplast InterZebTEC system: the first attempt to simplify and standardize the methodologies of microbiological investigation in aquatic species by offering the possibility of introducing a simple health and hygiene monitoring routine with a standardized sample collection methodology. Moderator: Marco Brocca (Italy) The Tecniplast InterZebTEC: a Novel Technical Approach to the Environmental Microbiological Monitoring of Zebrafish. Speaker: Gianpaolo Milite (Italy) Scientific Session: Germ Line, Early Development and Patterning I 11:40–12:45 VIRTUAL ROOM A Chair: Ferenc Mueller (United Kingdom) 11:40 A Newly Identified Cilium Mechanically Controls Meiotic Chromosomal Pairing and Germ Cell Morphogenesis in Zebrafish And Mouse Yaniv Elkouby (Israel) 11:55 Rbpms2 function in plasticity and sex-specific differentiation of germ cells Florence Marlow (USA) 12:07 Real-time monitoring of Fgf8 propagation during Zebrafish gastrulation and validation of its role as a morphogen Rohit Krishnan Harish (Germany) 12:19 Nodal signalling defines a competency window for the separation of the endodermal from mesodermal lineage through stochastic switching in cell fate Andrew Economou (United Kingdom) 5 Scientific Session: Neurobiology I 11:40–12:45 VIRTUAL ROOM B Chair: Bettina Schmid (Germany) 11:40 Shining a light on the enteric nervous system Gilles Vanwalleghem (Australia) 11:55 Gigaxonin-E3 ligase controls the initiation of Sonic Hedgehog signaling to sustain motor neuron specification and motility in zebrafish Pascale Bomont (France) 12:07 Purinergic signaling selectively modulates maintenance but not repair neurogenesis in the zebrafish olfactory epithelium Mehmet Can Demirler (Turkey) 12:19 Regulation of axonal growth and synaptogenesis by the cytoplasmic pool of core spliceosomal protein SNRNP70 Nikolas Nikolaou (United Kingdom) Keynote Session: Keynote Session – Prof. Dr. Nikolaus Rajewsky 13:30–14:30 VIRTUAL ROOM A Chair: Philipp Junker (Germany) Single-Cell Approaches in Medicine Nikolaus Rajewsky (Germany) Scientific Session: Emerging Technologies 14:30–15:35 VIRTUAL ROOM A Chair: Petr Bartunek (Czech Republic) 14:30 The Flamingo project makes custom, high-end microscopy more accessible Michael Weber (USA) 14:45 Harmonic generation in-vivo imaging of the zebrafish cardiovascular system Guy Malkinson (France) 14:57 Single-cell phenotypic analysis and dual lineage tracing in real-time with a zebrafish genetic mosaic system Bing Xu (USA) 6 15:09 Cre-controlled CRISPR (3C) provides an easy method for conditional gene inactivation in zebrafish Stefan Hans (Germany) Scientific Session: Community Session 15:45–16:40 VIRTUAL ROOM A Stefan Schulte-Merker (Germany) Elizabeth Patton (United Kingdom) Carole Wilson (United Kingdom) Kevin Thiessen (Germany) Periklis Pantazis (United Kingdom) Apolline Goudmaeker (Belgium) Tatjana Piotrowski (USA) Scientific Session: Disease Models I 17:00–18:05 VIRTUAL ROOM A Chair: Ewa Snaar-Jagalska 17:00 Functional evaluation of catastrophic epilepsy genes: The Epilepsy Zebrafish Project (EZP) Scott Baraban (USA) 17:15 Genetic variants associated to type 2 diabetes modulate endocrine enhancers in vivo Ana Eufrásio (Portugal) 17:27 Abnormal spine development and highly mineralized inclusions in vertebrae in a zebrafish model of CHARGE syndrome Maximilian Breuer (Canada) 17:39 Istaroxime treatment ameliorates calcium dysregulation in a zebrafish model for Phospholamban R14del cardiomyopathy Sarah Kamel (Netherlands) Scientific Session: Husbandry and Aquaculture 17:00–18:05 VIRTUAL ROOM B Chair: Christian Lawrence (USA) 17:00 Anesthesia does not equal analgesia: pain management in
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