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Constraints on Black-Hole Charges with the 2017 EHT Observations of M87*
PHYSICAL REVIEW D 103, 104047 (2021) Constraints on black-hole charges with the 2017 EHT observations of M87* – Prashant Kocherlakota ,1 Luciano Rezzolla,1 3 Heino Falcke,4 Christian M. Fromm,5,6,1 Michael Kramer,7 Yosuke Mizuno,8,9 Antonios Nathanail,9,10 H´ector Olivares,4 Ziri Younsi,11,9 Kazunori Akiyama,12,13,5 Antxon Alberdi,14 Walter Alef,7 Juan Carlos Algaba,15 Richard Anantua,5,6,16 Keiichi Asada,17 Rebecca Azulay,18,19,7 Anne-Kathrin Baczko,7 David Ball,20 Mislav Baloković,5,6 John Barrett,12 Bradford A. Benson,21,22 Dan Bintley,23 Lindy Blackburn,5,6 Raymond Blundell,6 Wilfred Boland,24 Katherine L. Bouman,5,6,25 Geoffrey C. Bower,26 Hope Boyce,27,28 – Michael Bremer,29 Christiaan D. Brinkerink,4 Roger Brissenden,5,6 Silke Britzen,7 Avery E. Broderick,30 32 Dominique Broguiere,29 Thomas Bronzwaer,4 Do-Young Byun,33,34 John E. Carlstrom,35,22,36,37 Andrew Chael,38,39 Chi-kwan Chan,20,40 Shami Chatterjee,41 Koushik Chatterjee,42 Ming-Tang Chen,26 Yongjun Chen (陈永军),43,44 Paul M. Chesler,5 Ilje Cho,33,34 Pierre Christian,45 John E. Conway,46 James M. Cordes,41 Thomas M. Crawford,22,35 Geoffrey B. Crew,12 Alejandro Cruz-Osorio,9 Yuzhu Cui,47,48 Jordy Davelaar,49,16,4 Mariafelicia De Laurentis,50,9,51 – Roger Deane,52 54 Jessica Dempsey,23 Gregory Desvignes,55 Sheperd S. Doeleman,5,6 Ralph P. Eatough,56,7 Joseph Farah,6,5,57 Vincent L. -
The Role of Neural Crest in Vertebrate Evolution
The Role of Neural Crest in Vertebrate Evolution: Tissue-specific Genetic Labeling in Zebrafish (Danio rerio) Reveals Neural Crest Contribution to Gills, but Not to Scales Dissertation der Mathematisch-Naturwissenschaftlichen Fakultät der Eberhard Karls Universität Tübingen zur Erlangung des Grades eines Doktors der Naturwissenschaften (Dr. rer. nat.) vorgelegt von Alessandro Mongera aus Trento (Italien) Tübingen 2013 Tag der mündlichen Qualifikation: 09.12.2013 Dekan: Prof. Dr. Wolfgang Rosenstiel 1. Berichterstatter: Prof. Dr. Christiane Nüsslein-Volhard 2. Berichterstatter: Prof. Dr. Rolf Reuter 3. Berichterstatter: Dr. Didier Stainier to my friends Contents Abstract 7 Zusammenfassung 11 1 Introduction 15 1.1 Neural crest as a source of vertebrate innovations 15 1.2 Tools for cell lineage tracing . 18 1.2.1 Non-genetic labeling . 18 1.2.2 Cre/loxP-mediated genetic labeling . 21 1.3 The gills . 23 1.4 The post-cranial integumentary skeleton . 25 2 Results 29 Publication 1 . 29 Publication 2 . 43 3 Discussion 49 3.1 NC-driven expansion of the respiratory surface 49 3.2 Mesoderm, and not NC, contributes to fish scales 52 3.3 Tg(sox10:ERT2-Cre) line as a tool to under- stand vertebrate evolution . 55 Bibliography 56 5 Own contribution to the manuscripts 69 Curriculum vitae 71 Full publication list 73 Acknowledgments 75 Appendix 77 Publication 3 . 79 Publication 4 . 95 6 Abstract During vertebrate evolution, the development of an active, predatory lifestyle and an increased body size coincided with the appearance of many morphological innovations. Among these vertebrate-specific phenotypic features, a major role in promoting the massive radiation of this animal group has been played by a new, true head. -
Transcriptional Adaptation in Caenorhabditis Elegans
RESEARCH ARTICLE Transcriptional adaptation in Caenorhabditis elegans Vahan Serobyan1*, Zacharias Kontarakis1†, Mohamed A El-Brolosy1, Jordan M Welker1, Oleg Tolstenkov2,3‡, Amr M Saadeldein1, Nicholas Retzer1, Alexander Gottschalk2,3,4, Ann M Wehman5, Didier YR Stainier1* 1Department of Developmental Genetics, Max Planck Institute for Heart and Lung Research, Bad Nauheim, Germany; 2Institute for Biophysical Chemistry, Goethe University, Frankfurt Am Main, Germany; 3Cluster of Excellence Frankfurt - Macromolecular Complexes (CEF-MC), Goethe University, Frankfurt Am Main, Germany; 4Buchmann Institute for Molecular Life Sciences (BMLS), Goethe University, Frankfurt Am Main, Germany; 5Rudolf Virchow Center, University of Wu¨ rzburg, Wu¨ rzburg, Germany Abstract Transcriptional adaptation is a recently described phenomenon by which a mutation in *For correspondence: one gene leads to the transcriptional modulation of related genes, termed adapting genes. At the [email protected] molecular level, it has been proposed that the mutant mRNA, rather than the loss of protein (VS); function, activates this response. While several examples of transcriptional adaptation have been [email protected] (DYRS) reported in zebrafish embryos and in mouse cell lines, it is not known whether this phenomenon is observed across metazoans. Here we report transcriptional adaptation in C. elegans, and find that † Present address: Genome this process requires factors involved in mutant mRNA decay, as in zebrafish and mouse. We Engineering and Measurement further uncover a requirement for Argonaute proteins and Dicer, factors involved in small RNA Lab, ETH Zurich, Functional maturation and transport into the nucleus. Altogether, these results provide evidence for Genomics Center Zurich of ETH transcriptional adaptation in C. -
Effelsberg Newsletter January 2012
Effelsberg Newsletter Volume 3 w Issue 1 w January 2012 Effelsberg Newsletter January 2012 Call for Proposals Deadline for Effelsberg Proposals is February 8, 2012, 13.00 UT. Page 2 Greetings from the Director Content Page 2 Call for Proposals It is a pleasure to wish you a very happy new year 2012! It has been a very busy last year, with lots of changes and new instrumentation Page 4 First results of the described in the past issues of this newsletter. Even more interesting Effelsberg-Bonn HI Survey changes lie ahead of us, and as usual, we will keep you posted about all the progress being made. We will also continue to give you a glimpse Page 6 Effelsberg delivers first of the science highlights achieved with the 100-m telescope, and it is fringes with Radioastron: also a pleasure to introduce you to further members of staff and to Space VLBI sets a new report on our outreach activities. world-record in radio interferometry. The output and the performance of the telescope is going strong, underlining its superb capabilities even 40 years after its inauguration. Page 7 Who is Who in Effelsberg? Peter Vogt This is not surprising, given that the telescope and its instruments are constantly upgraded. Still, we are only half-way through our activities Page 8 Public Outreach: celebrating these 40 years. Further highlights are still to follow - stay Extension of the tuned! We will give you further details in the upcoming issues of this Effelsberg Milky Way newsletter, which now has a new look. -
TWO POSTDOCTORAL POSITIONS in RADIO ASTRONOMY at the Mpifr in BONN, GERMANY Submission Address: Prof
TWO POSTDOCTORAL POSITIONS IN RADIO ASTRONOMY AT THE MPIfR IN BONN, GERMANY Submission address: Prof. Dr. J. Anton Zensus Max-Planck-Institut für Radioastronomie Auf dem Hügel 69 D-53123 Bonn, Germany Tel. +49 228 525 298 [email protected] Contact information for inquiries: Prof. Dr. Eduardo Ros Tel. +49 228 525 125 [email protected] Deadline: Dec 1st, 2017 The Max-Planck-Institut für Radioastronomie (MPIfR) is recruiting up to two postdoctoral reserachers in radio astronomy. These positions are based in the Radio Astronomy/VLBI department led by Prof. J. Anton Zensus. The department has a broad research portfolio. Research topics focus on the investigation of compact extragalactic objects (AGN) and their broad-band emission, with a special emphasis on very-high-resolution studies. These include mm- and space-VLBI imaging and blazar monitoring with F-GAMMA/MOJAVE/TANAMI. The MPIfR operates the 100-m radio telescope in Effelsberg (element of EVN and GMVA), and it is major partner of APEX in Chile (element of EHT). It also operates a state-of-the-art VLBI correlator and the LOFAR DE1 station, which is part of the International LOFAR Telescope. We are looking for researchers with a science interest matching our research topics. Technical mastery of radio interferometry techniques and VLBI is of advantage, as well as a good publication record. Candidates with complementary skills to our research portfolio are especially encouraged to apply. The appointee will be expected to engage in one or more of our research lines and to collaborate closely with other department members. The International Max Planck Research School for Astronomy and Astrophysics (IMPRS) offers the opportunity for joint research with PhD students, their supervision, and teaching in the program. -
Reaching an Agreement on UK Participation in Horizon Eu
Securing a strong outcome for research in the EU-UK future relationship: Reaching an agreement on UK participation in Horizon Europe The EU and UK have been discussing UK participation in Union Programmes, including Horizon Europe, as part of the negotiations on a future EU-UK relationship. It is encouraging that both sides have committed to the principle of UK participation in their mandates, recognising that collaboration between the UK and the EU in the framework programmes strengthens our ability to tackle shared challenges, such as cancer and climate change. It is vital that this commitment now translates quickly into an agreement on the terms of participation. Horizon Europe association should be a core part of the future relationship between the EU and the UK for research, underpinning valuable scientific partnerships that have been built up over many years. We have all reaped the health benefits of these collaborations. Clinical trials, particularly on diseases with limited patient populations, are reliant on EU-UK collaboration, while close research partnerships continue to accelerate life-changing medical research. Our ability to respond to the threat of climate change and outbreaks of new diseases like Covid-19 has also been greatly improved by close scientific and clinical partnerships across Europe. Knowledge and discovery do not stop at borders, and the shared global challenges we face require joint solutions. Collaboration through the research framework programmes is a springboard to productive partnerships across the world. We owe it to future generations in the UK, the EU and beyond to ensure that the new EU-UK relationship best serves them through research. -
A Portrait of a Black Hole
Overview_Black Holes A picture of a dark monster. The image is the first direct visual evidence of a black hole. This particularly massive specimen is at the center of the supergiant galaxy Messier 87 and was acquired by the Event Horizon Telescope (EHT), an array of eight ground-based radio telescopes distributed around the globe. A portrait of a black hole Black holes swallow all light, making them invisible. That’s what you’d think anyway, but astronomers thankfully know that this isn’t quite the case. They are, in fact, surrounded by a glowing disc of gas, which makes them visible against this bright background, like a black cat on a white sofa. And that’s how the Event Horizon Telescope has now succeeded in taking the first picture of a black hole. Researchers from the Max Planck Institute for Radio Astronomy in Bonn and the Institute for Radio Astronomy in the Millimeter Range (IRAM) in Grenoble, France, were among those making the observations. Photo: EHT Collaboration TEXT HELMUT HORNUNG n spring 2017, scientists linked up objects at extremely small angles of less tion of the Max Planck Institute for Ra- eight telescopes spread out over than 20 microarcseconds. With that dio Astronomy. In the 2017 observing one face of the globe for the first resolution, our eyes would see individ- session alone, the telescopes recorded time, forming a virtual telescope ual molecules on our own hands. approximately four petabytes of data. with an effective aperture close to This is such a huge volume that it was the Idiameter of the entire planet. -
Transcriptional Adaptation in Caenorhabditis Elegans
Research Collection Journal Article Transcriptional adaptation in Caenorhabditis elegans Author(s): Serobyan, Vahan; Kontarakis, Zacharias; El-Brolosy, Mohamed A.; Welker, Jordan M.; Tolstenkov, Oleg; Saadeldein, Amr M.; Retzer, Nicholas; Gottschalk, Alexander; Wehman, Ann M.; Stainier, Didier Y.R. Publication Date: 2020 Permanent Link: https://doi.org/10.3929/ethz-b-000396015 Originally published in: eLife 9, http://doi.org/10.7554/eLife.50014 Rights / License: Creative Commons Attribution 4.0 International This page was generated automatically upon download from the ETH Zurich Research Collection. For more information please consult the Terms of use. ETH Library RESEARCH ARTICLE Transcriptional adaptation in Caenorhabditis elegans Vahan Serobyan1*, Zacharias Kontarakis1†, Mohamed A El-Brolosy1, Jordan M Welker1, Oleg Tolstenkov2,3‡, Amr M Saadeldein1, Nicholas Retzer1, Alexander Gottschalk2,3,4, Ann M Wehman5, Didier YR Stainier1* 1Department of Developmental Genetics, Max Planck Institute for Heart and Lung Research, Bad Nauheim, Germany; 2Institute for Biophysical Chemistry, Goethe University, Frankfurt Am Main, Germany; 3Cluster of Excellence Frankfurt - Macromolecular Complexes (CEF-MC), Goethe University, Frankfurt Am Main, Germany; 4Buchmann Institute for Molecular Life Sciences (BMLS), Goethe University, Frankfurt Am Main, Germany; 5Rudolf Virchow Center, University of Wu¨ rzburg, Wu¨ rzburg, Germany Abstract Transcriptional adaptation is a recently described phenomenon by which a mutation in *For correspondence: one gene leads to the transcriptional modulation of related genes, termed adapting genes. At the [email protected] molecular level, it has been proposed that the mutant mRNA, rather than the loss of protein (VS); function, activates this response. While several examples of transcriptional adaptation have been [email protected] (DYRS) reported in zebrafish embryos and in mouse cell lines, it is not known whether this phenomenon is observed across metazoans. -
Role of Npas4l and Hif Pathway in Endothelial Cell Specification and Specialization in Vertebrates
Role of npas4l and Hif pathway in endothelial cell specification and specialization in vertebrates Dissertation zur Erlangung des Doktorgrades der Naturwissenschaften vorgelegt beim Fachbereich 15 der Goethe-Universität in Frankfurt am Main von Michele Marass aus Trieste, Italien Frankfurt 2018 (D30) vom Fachbereich Biowissenschaften (FB15) der Johann Wolfgang Goethe - Universität als Dissertation angenommen. Dekan: Prof. Dr. Sven Klimpel Gutachter: Prof. Dr. Didier Y. R. Stainier Prof. Dr. Virginie Lecaudey Datum der Disputation : 2 REVIEWERS Prof. Dr. Didier Stainier, Ph.D. Department of Developmental Genetics Max Planck Institute for Heart and Lung Research Bad Nauheim, Germany and Prof. Dr. Virginie Lecaudey, Ph.D. Department of Developmental Biology of Vertebrates Institute of Cell Biology and Neuroscience Johann Wolfgang Goethe University Frankfurt am Main, Germany 3 ERKLÄRUNG Ich erkläre hiermit, dass ich mich bisher keiner Doktorprüfung im Mathematisch-Naturwissenschaftlichen Bereich unterzogen habe. Frankfurt am Main, den ............................................ (Unterschrift) Versicherung Ich erkläre hiermit, dass ich die vorgelegte Dissertation über Role of npas4l and Hif pathway in endothelial cell specification and specialization in vertebrates selbständig angefertigt und mich anderer Hilfsmittel als der in ihr angegebenen nicht bedient habe, insbesondere, dass alle Entlehnungen aus anderen Schriften mit Angabe der betreffenden Schrift gekennzeichnet sind. Ich versichere, die Grundsätze der guten wissenschaftlichen Praxis -
The Zebrafish Issue of Development Christiane Nüsslein-Volhard*
SPOTLIGHT 4099 Development 139, 4099-4103 (2012) doi:10.1242/dev.085217 © 2012. Published by The Company of Biologists Ltd The zebrafish issue of Development Christiane Nüsslein-Volhard* Summary had just started, and it was by no means obvious to what degree In December 1996, a special issue of Development appeared that invertebrates and vertebrates were related – the common notion presented in 37 papers the results of two large screens for was rather not at all, as their modes of development appeared to be zebrafish mutants performed in Tübingen and Boston. The so different. The way embryonic development is described is papers describe about 1500 mutations in more than 400 new dictated by the methods used for analysis. Thus, in flies, genes involved in a wide range of processes that govern development seemed to be determined by genes (defined by development and organogenesis. Up to this day, the mutants mutants), whereas in frogs, enigmatic factors postulated from provide a rich resource for many laboratories, and the issue experimental manipulations did the job. So it was conceivable that significantly augmented the importance of zebrafish as the work on flies would not help us to understand frog (i.e. vertebrate model organism for the study of embryogenesis, vertebrate) development. Still, I was convinced that genetics was neuronal networks, regeneration and disease. This essay relates the most successful approach to dissect and understand complex a personal account of the history of this unique endeavor. systems. The mutant and its phenotype, caused by the lack of a single gene product, is a cleaner experiment than any Introduction transplantation, constriction or centrifugation could ever be. -
Michal Zajaček – Curriculum Vitae
MPIfR Bonn Ph1, Universität zu Köln Michal Zajaček T +49(0)221/470-7788 u +49(0)221/470-6727 B [email protected] Curriculum Vitae Í www.astro.uni-koeln.de/∼zajacek Education September 17, PhD degree, IMPRS scholar at University of Cologne, 1. Physikalisches Institut and MPIfR, 2014– October Bonn, Germany, graduated as very good – sehr good with the grade 1.00. 12, 2017 December 15, Rigorosum examination – RNDr. (Czech Title for the Doctor of natural sciences), 2014 Charles University in Prague, Czech Republic. 2012–2014 Master of Science (Astronomy & Astrophysics), Charles University in Prague, Czech Republic, graduated with honours. 2009–2012 Bachelor of Science (General Physics), Charles University in Prague, Czech Republic, graduated with honours. 2004–2009 Abitur (Maturita), Evangelical Lyceum in Bratislava, Slovakia, all grades excellent. PhD Thesis Title Interaction between interstellar medium and black-hole environment Supervisor Prof. Dr. Andreas Eckart Advisors PD Dr. Silke Britzen, Prof. Dr. Vladimír Karas, Dr. Mónica Valencia-S., Prof. Dr. J. Anton Zensus Description The thesis deals with the transition region where the complex interstellar medium in the Galactic Centre meets a rather simple object at the very centre – most probably a black hole of 4 × 106 Solar masses characterized by only three classical parameters: mass, spin, and electric charge. Recently, a NIR-excess object named DSO/G2 was detected that is moving on a highly eccentric orbit, with the pericentre reached in 2014 at ∼ 2000 Schwarzschild radii. The monitoring, the analysis of NIR data, and the comparison with models have provided an unprecedented opportunity to constrain the properties of previously unexplored region around Sgr A* as well as to determine the nature of this enigmatic source. -
Development in Zebrafish, a Genetic Approach
Development in Zebrafish, a Genetic Approach Didier Stainier University of California [email protected] Zebrafish genetics Didier Stainier, UCSF Biochemistry Juquehy, Brazil 5/2/05 Saccharomyces cerevisiae Caenorhabditis elegans Drosophila melanogaster Saccharomyces cerevisiae Caenorhabditis elegans Drosophila melanogaster Danio rerio GENETIC SCREENS What can one screen for? - developmental processes - physiological processes -behavior -other external fertilization some key developmental stages Zebrafish embryonic development QuickTime™ and a Animation decompressor are needed to see this picture. large number of progeny screening based on morphological traits Zebrafish embryos showing abnormal DV patterning V; ventralized chordin mutants C; dorsalized bmp2 mutants tolloid mutants Cardiac valve formation QuickTime™ and a QuickTime™ and a Video decompressor Video decompressor are needed to see this picture. are needed to see this picture. QuickTime™ and a Cinepak decompressor are needed to see this picture. AP staining flk1:GFP 52 hpf gutGFP line Liver Swim bladder Pancreas Intestinal bulb Ventral View QuickTime™ and a Motion JPEG A decompressor are needed to see this picture. Duc Dong 80 hpf L QuickTime™ and a Motion JPEG A decompressor are needed to see this picture. P lfabp-dsRed elastase-GFP insulin-dsRed Ryan Anderson forward genetic screens in zebrafish -ENU - insertional mutagenesis (retroviral vectors) - large number of tanks (diploid screens) - 3-4 scientists - 18-24 months What can you do with your mutants? - clone the