Tender Enquiry No: 8-61/Stores/LHMC/AT/2020-21
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PAPANICOLAOU STAINING SYSTEM (Procedure No. HT40)
PAPANICOLAOU 6. Tap water......................................................................................................................rinse STAINING SYSTEM 7. Scott’s Tap Water Substitute................................................................................10 dips (Procedure No. HT40) 8. Tap water......................................................................................................................rinse 9. ReagentAlcohol,95%.........................................................................................10dips _______________________________________________ 10. PapanicolaouStainOG6..............................................................................1.5minutes INTENDED USE 11. ReagentAlcohol,95%.........................................................................................10dips _______________________________________________ 12. PapanicolaouStainModifiedEA,OR PapanicolaouStainEA50,OR The Sigma-Aldrich Papanicolaou Staining system is intended for staining exfoliative PapanicolaouStainEA65.............................................................................2.5minutes cells in cytologic specimens. Papanicolaou staining reagents are for “In Vitro Diagnostic 13. ReagentAlcohol,95%,twochanges..........................................................10dipseach Use.” 1 14. ReagentAlcohol,100%.....................................................................................1minute Papanicolaou staining techniques, reviewed in a concise report -
New Tetrachromic VOF Stain (Type III-G.S) for Normal and Pathological Fish Tissues C
ORIGINAL PAPER New Tetrachromic VOF Stain (Type III-G.S) for Normal and Pathological Fish Tissues C. Sarasquete,* M. Gutiérrez Instituto de Ciencias Marinas de Andalucía, CSIC Polígono Río San Pedro, Apdo oficial, Puerto Real, Cádiz, Spain richrome methods invariably use dyes in acid ©2005, European Journal of Histochemistry pH solvents, usually diluted in aqueous acetic Tacid, and the concentration of this acid A new VOF Type III-G.S stain was applied to histological sec- matches the concentration of dye. Staining depends tions of different organs and tissues of healthy and pathologi- largely on the attachment of dyes to proteins. The cal larvae, juvenile and adult fish species (Solea senegalensis; acid pH itself is necessary to maximise the amount Sparus aurata; Diplodus sargo; Pagrus auriga; Argyrosomus regius and Halobatrachus didactylus). In comparison to the of dye that will attach to tissue amino groups. original Gutiérrez´VOF stain, more acid dyes of contrasting Proteins have both positively (amino groups) and colours and polychromatic/metachromatic properties were negatively (carboxyl and hydroxyl) charged groups. incorporated as essential constituents of the tetrachromic VOF Usually one predominates and this will have an stain. This facilitates the selective staining of different basic tissues and improves the morphological analysis of histo- overall negative or positive charge (being an acid or chemical approaches of the cell components. The VOF-Type III a basic protein). These charges can, however, bal- G.S stain is composed of a mixture of several dyes of varying ance each other out to some degree. Phosphate size and molecular weight (Orange G< acid Fuchsin< Light green<Methyl Blue<Fast Green), which are used simultane- groups of DNA and binding-proteins are important ously, and it enables the individual tissues to be selectively dif- in nuclear staining.The ionisation of basic groups of ferentiated and stained. -
Reticulin Stain
US 20110229879A1 (19) United States (12) Patent Application Publication (10) Pub. No.: US 2011/0229879 A1 CHURUKIAN (43) Pub. Date: Sep. 22, 2011 (54) METHODS AND COMPOSITIONS FOR Publication Classification NUCLEAR STANING (51) Int. Cl. (75) Inventor: Charles J. CHURUKIAN, CI2O I/68 (2006.01) Rochester, NY (US) (52) U.S. Cl. ......................................................... 435/6.1 (73) Assignee: UNIVERSITY OF ROCHESTER, Rochester, NY (US) (57) ABSTRACT The present invention relates to compositions, methods, and (21) Appl. No.: 13/052,791 kits Suitable for detecting nucleic acids inabiological sample. The nuclear staining composition of the present invention (22) Filed: Mar. 21, 2011 contains a pH buffering reagent, a solubilizing reagent, a basic dye, and an aqueous medium. The composition can be Related U.S. Application Data used alone to detect nucleic acids in a biological sample or in (60) Provisional application No. 61/315,483, filed on Mar. combination with other histological dyes for nuclear counter 19, 2010. staining. Reticulin Stain Patent Application Publication Sep. 22, 2011 Sheet 1 of 3 US 2011/0229879 A1 Reticulin Stain Figures 1A-1B Patent Application Publication Sep. 22, 2011 Sheet 2 of 3 US 2011/0229879 A1 Iron Stain Figures 2A-2B Patent Application Publication Sep. 22, 2011 Sheet 3 of 3 US 2011/0229879 A1 Alcian Blue Figures 3A-3B US 2011/0229.879 A1 Sep. 22, 2011 METHODS AND COMPOSITIONS FOR composition of the present invention does not precipitate in NUCLEAR STANING Solution and has a shelf-life of at least one year. In addition, nuclear staining with the composition of the present invention achieves brighter, more brilliant nuclear staining showing 0001. -
20 to 30 Sec)
457 Observations on a highly specific method for the histochemical detection of sulphated mucopolysaccharides, and its possible mechanisms By I. D. HEATH (From the Department of Anatomy, University of St. Andrews, Queen's College, Dundee. Present address: General Hospital, Nottingham) With 3 plates (figs, i to 3) Summary Whereas basic dyes in aqueous solutions stain chromatin, all mucins, mast cells, the ground substance of cartilage, and epidermis, it has been shown that a 0-03 % solution of basic dye in 5% aluminium sulphate produces a highly specific staining reaction for sulphated mucopolysaccharides. The best dyes are nuclear fast red (Herzberg) and methylene blue. Acid dyes in solutions of aluminium salts are induced to stain the ground substance of cartilage. These observations have been confirmed in a num- ber of species. Other metallic ions have similar properties and the use of green and purple chromic salts indicate that co-ordination plays a part in the reaction. Methylation, saponification, and sulphation experiments show that the sulphate group is essential. This has been confirmed by using pure chemical substances in gelatin models. Oxidation of keratin with performic acid, which produces sulphonic groups, causes hair (previously negative) to react. From this it is suggested that sul- phonic groups may also react, and that the reactive groups need not be attached to mucopolysaccharides. It is further suggested that the specificity of sulphated muco- polysaccharides is due to the fact that they are the only substances present in the tis- sues with a sufficient concentration of sulphate groups. Experiments with solochrome azurine show that the aluminium is attached to all tissue elements irrespective of their nature. -
Biological Stains & Dyes
BIOLOGICAL STAINS & DYES Developed for Biology, microbiology & industrial applications ACRIFLAVIN ALCIAN BLUE 8GX ACRIDINE ORANGE ALIZARINE CYANINE GREEN ANILINE BLUE (SPIRIT SOLUBLE) www.lobachemie.com BIOLOGICAL STAINS & DYES Staining is an important technique used in microscopy to enhance contrast in the microscopic image. Stains and dyes are frequently used in biology and medicine to highlight structures in biological tissues. Loba Chemie offers comprehensive range of Stains and dyes, which are frequently used in Microbiology, Hematology, Histology, Cytology, Protein and DNA Staining after Electrophoresis and Fluorescence Microscopy etc. Many of our stains and dyes have specifications complying certified grade of Biological Stain Commission, and suitable for biological research. Stringent testing on all batches is performed to ensure consistency and satisfy necessary specification particularly in challenging work such as histology and molecular biology. Stains and dyes offer by Loba chemie includes Dry – powder form Stains and dyes as well as wet - ready to use solutions. Features: • Ideally suited to molecular biology or microbiology applications • Available in a wide range of innovative chemical packaging options. Range of Biological Stains & Dyes Product Code Product Name C.I. No CAS No 00590 ACRIDINE ORANGE 46005 10127-02-3 00600 ACRIFLAVIN 46000 8063-24-9 00830 ALCIAN BLUE 8GX 74240 33864-99-2 00840 ALIZARINE AR 58000 72-48-0 00852 ALIZARINE CYANINE GREEN 61570 4403-90-1 00980 AMARANTH 16185 915-67-3 01010 AMIDO BLACK 10B 20470 -
Magenta and Magenta Production
MAGENTA AND MAGENTA PRODUCTION Historically, the name Magenta has been used to refer to the mixture of the four major constituents comprising Basic Fuchsin, namely Basic Red 9 (Magenta 0), Magenta I (Rosaniline), Magenta II, and Magenta III (New fuchsin). Although samples of Basic Fuchsin can vary considerably in the proportions of these four constituents, today each of these compounds except Magenta II is available commercially under its own name. Magenta I and Basic Red 9 are the most widely available. 1. Exposure Data 1.1 Chemical and physical data 1.1.1 Magenta I (a) Nomenclature Chem. Abstr. Serv. Reg. No.: 632–99–5 CAS Name: 4-[(4-Aminophenyl)(4-imino-2,5-cyclohexadien-1-ylidene)methyl]-2- methylbenzenamine, hydrochloride (1:1) Synonyms: 4-[(4-Aminophenyl)(4-imino-2,5-cyclohexadien-1-ylidene)methyl]-2- methylbenzenamine, monohydrochloride; Basic Fuchsin hydrochloride; C.I. 42510; C.I. Basic Red; C.I. Basic Violet 14; C.I. Basic Violet 14, monohydrochloride; 2- methyl-4,4'-[(4-imino-2,5-cyclohexadien-1-ylidene)methylene]dianiline hydrochloride; rosaniline chloride; rosaniline hydrochloride –297– 298 IARC MONOGRAPHS VOLUME 99 (b) Structural formula, molecular formula, and relative molecular mass NH HCl H2N NH2 CH3 C20H19N3.HCl Rel. mol. mass: 337.85 (c) Chemical and physical properties of the pure substance Description: Metallic green, lustrous crystals (O’Neil, 2006; Lide, 2008) Melting-point: Decomposes above 200 °C (O’Neil, 2006; Lide, 2008) Solubility: Slightly soluble in water (4 mg/mL); soluble in ethanol (30 mg/mL) and ethylene -
United States Patent Office Patented Jan
3,488,705 United States Patent Office Patented Jan. 6, 1970 2 3,488,705 having desirable electrophotographic properties can be THERMALLY UNSTABLE ORGANIC ACD SALTS especially useful in elecetrophotography. Such electro OF TRARYLMETHANE DYES AS SENSTEZERS photographic elements may be exposed through a trans FOR ORGANIC PHOTOCONDUCTORS parent base if desired, thereby providing unusual flexibility Charles J. Fox and Arthur L. Johnson, Rochester, N.Y., in equipment design. Such compositions, when coated as assignors to Eastman Kodak Company, Rochester, a film or layer on a suitable support also yield an element N.Y., a corporation of New Jersey No Drawing. Continuation-in-part of application Ser. No. which is reusable; that is, it can be used to form subse 447,937, Mar. 16, 1965. This application Dec. 4, 1967, quent images after residual toner from prior images has Ser. No. 687,503 been removed by transfer and/or cleaning. nt. C. G03g 5/06, 9/00 0. Although some of the organic photoconductors com U.S. C. 96-1.6 32 Claims prising the materials described are inherently light sensi tive, their degree of sensitivity is usually low and in the short wavelength portion of the spectrum so that it is ABSTRACT OF THE DISCLOSURE common practice to add materials to increase the speed 15 and to shift the sensitivity toward the longer wavelength Organic acid salts of triarylmethane dyes are useful as portion of the visible spectrum. Increasing the speed and sensitizers in electrophotographic elements. They are shifting the sensitivity of such systems into the visible thermally unstable and thus readily bleachable. -
A Great Variety of Sources Has Been Used in the Preparation of This Lab Manual, Including Textbooks, Journals, Other Laboratory Manuals, Personal Communications, Etc
A great variety of sources has been used in the preparation of this lab manual, including textbooks, journals, other laboratory manuals, personal communications, etc. Since the material contained in this manual is still undergoing modification and revision, the references have not yet been included. When the evolution nears completion, proper sources will be cited. I am especially indebted to Dr. A.A. Weaver of The College of Wooster, for initiating my interest in histology and microtechnique. It is his manual which provided a lot of the material contained in this book. I am also indebted to Sue Beaumier for her tireless typing and retyping of this text TABLE OF CONTENTS INTRODUCTION 4 TOXIC SUBSTANCES 5 SECTION I MICROTECHNIQUE: TISSUE PREPARATION Killing and Fixation 13 Fixative Formulae 17 Primary Fixatives 18 Dehydration 19 Clearing 20 Paraffin Infiltration 21 Alternate Schedule for Dehydration, Clearing and Embedding 22 Embedding 23 Sectioning 24 Mounting Sections on Slides 25 SECTION II STAINING Staining 28 Dehydration, Clearing and Mounting 28 Cleaning and Labeling Slides 29 Characteristics of a Good Slide 30 General Procedures 32 STAIN PROTOCOLS: Nuclear Stains - Hematoxylins Indirect Hematoxylins 35 Direct Hematoxylins 37 Cytoplasmic Stains - Counterstains 39 Multiple Contrast Stains Picro-Mallory 41 Masson Trichrome 43 Gabe’s Trichrome 44 Acidic & Basic Tissue Components 45 Effect of pH on Staining 46 Metachromasia - Toluidine blue 48 Technique for Nucleic Acids Feulgen (Nucleal) Test 50 Basic Dye Staining of RNA 52 Methyl Green-Pyronin -
The Color of Tissue Diagnostics Routine Stains, Special Stains and Ancillary Reagents
The Color of Tissue Diagnostics Routine Stains, Special Stains and Ancillary Reagents The life science business of Merck KGaA, Darmstadt, Germany operates as MilliporeSigma in the U.S. and Canada. For over years, 100routine stains, special stains and ancillary reagents have been part of the MilliporeSigma product range. This tradition and experience has made MilliporeSigma one of the world’s leading suppliers of microscopy products. The products for microscopy, a comprehensive range for classical hematology, histology, cytology, and microbiology, are constantly being expanded and adapted to the needs of the user and to comply with all relevant global regulations. Many of MilliporeSigma’s microscopy products are classified as in vitro diagnostic (IVD) medical devices. Quality Means Trust As a result of MilliporeSigma’s focus on quality control, microscopy products are renowned for excellent reproducibility of results. MilliporeSigma products are manufactured in accordance with a quality management system using raw materials and solvents that meet the most stringent quality criteria. Prior to releasing the products for particular applications, relevant chemical and physical parameters are checked along with product functionality. The methods used for testing comply with international standards. For over Contents Ancillary Reagents Microbiology 3-4 Fixing Media 28-29 Staining Solutions and Kits years, 5-6 Embedding Media 30 Staining of Mycobacteria 100 6 Decalcifiers and Tissue Softeners 30 Control Slides 7 Mounting Media Cytology 8 Immersion -
Dye Origins Hematoxylin Is Extracted from the Logwood Tree and Purified. It Is Then Oxidized and Combined with a Mordant (Typica
Dye Origins Hematoxylin is extracted from the logwood tree and purified. It is then oxidized and combined with a mordant (typically aluminum) to allow it to bind to the cell structures. Of the many hematoxylin preparations used in histology Gill’s hematoxylin, Harris's hematoxylin and Mayer's hematoxylin are the most popular. Eosin is formed by a reaction between bromine and fluorescein. There are two eosin variants typically used in histology: eosin Y which is slightly yellowish and eosin B which is slightly bluish. Eosin Y is most popular. Special Stains The term special stains traditionally referred to any staining other than an H&E. It covers a wide variety of methods that may be used to visualize particular tissue structures, elements, or even microorganisms not identified by H&E staining. Other methods of staining use immunohistochemistry or in situ hybridization to target specific proteins or DNA/RNA sequences. These methods were sometimes also included as members of the “special stains” family. However, they are quite different in method and purpose and are now typically separated into a third category known as “advanced stains”. While there are literally hundreds of special stains for all manner of purposes, only a few are used with any regularity in clinical histology. The variety of stains also means that special staining is not as automated as H&E staining. While many larger laboratories do use automated instruments for the more common stains, they still have an area for hand staining. The complexity of some stains also works against the uses of automation. Some Common Special Stains. -
Chemical Inventory Contents
HISTOLOGY LABORATORY CHEMICAL INVENTORY CONTENTS 1 Carcinogens ..........................................................................................................3 1.1 Stains 1.2 Carcinogenic determination 2 Extremely hazardous .....................................................................................4 2.1 Reproductive toxins 2.2 High acute toxidity 3 Acid cabinet .........................................................................................................4 4 Chemical cabinet ..............................................................................................5 5 Flammables room .............................................................................................7 6 Histology refrigerator .................................................................................8 7 Histology laboratory .....................................................................................8 8 Stain cabinet ....................................................................................................10 Chemical Inventory 2 1 Carcinogens Product / Common Name Location MSDS Filed Aniline Chloroform Chromium compounds Chromic acid Potassium dichromate Formaldehyde 1.1 Stains Product / Common Name Location MSDS Filed Auramine O, CI 41000 Basic fuchsin, CI 42500 (pararosaniline hydrochloride) Chlorazol black E, CI 30235 Ponceau de xylidine, CI 16150 (ponceau 2R) 1.2 Carcinogenic determination These chemicals are carcinogenic to animals, though there is no human data. Product / Common Name Location MSDS -
Important Early Synthetic Dyes
IMPORTANT EARLY SYNTHETIC DYES Chemistry Constitution Date Properties Compiled by Textile Conservation Interns Andrea Bowes Stephen Collins Shannon Elliott La Tasha Harris Laura Hazl ett Ester M~ th~ Muhammadin Razak Pugi Yosep Subagiyo Edited by Mary W. Ballard, Senior Textile Conservator Conservation Analytical Laboratory Smithsonian Institution 1991 Preface This notebook presents information on a limited number of important early synthetic dyes as recommended by the extensive work of Dr. Helmut Schweppe in the field of dye analysis. A great deal of the data has been abstracted from the well-known encyclopedia on dye structure and properties, the Colour Index, published jointly by the American Association of textile Chemists and Colorists and the Society of Dyers and Colourists. The purpose of this manual is as an aid to textile conservators in their laboratories. It is distributed by the Conservation Analytical Laboratory, Smithsonian Institution, with the kind permission of the American Association of Textile Chemists and Colorists. For a complete review of all known dye structures and chemical properties of dyes, the reader is referred to the master volumes of the Colour Index. Mary W. Ballard, editor Senior Textile Conservator, CAL Senior Member, AATCC October, 1991 TABLE OF CONTENTS I). Listing of Early Synthetic Dyes (by Colour Index Number) (in chronological order by color) II). Glossary of Terms III). Chemistry, Constitution, Date, and Properties Bibliography IV). Toxicity Information a). General Health Hazard Protective Equipment b). Accidental Contact Occurrence General Procedures c). Toxicity Manufacturers Listing d). Toxicity Bibliography V). Chemistry, Constitution, Date and Properties Dye Information Section (Arrange by Colour Index Number) Commercial Name: Colour Index Discoverer, year: Generic Name: C.I.Number: 1) Picric Acid Acid Dye 10305 (Woulfe,l771) 2) Martius Yellow C.I.Acid Yello..