Reticulin Stain
US 20110229879A1 (19) United States (12) Patent Application Publication (10) Pub. No.: US 2011/0229879 A1 CHURUKIAN (43) Pub. Date: Sep. 22, 2011 (54) METHODS AND COMPOSITIONS FOR Publication Classification NUCLEAR STANING (51) Int. Cl. (75) Inventor: Charles J. CHURUKIAN, CI2O I/68 (2006.01) Rochester, NY (US) (52) U.S. Cl. ......................................................... 435/6.1 (73) Assignee: UNIVERSITY OF ROCHESTER, Rochester, NY (US) (57) ABSTRACT The present invention relates to compositions, methods, and (21) Appl. No.: 13/052,791 kits Suitable for detecting nucleic acids inabiological sample. The nuclear staining composition of the present invention (22) Filed: Mar. 21, 2011 contains a pH buffering reagent, a solubilizing reagent, a basic dye, and an aqueous medium. The composition can be Related U.S. Application Data used alone to detect nucleic acids in a biological sample or in (60) Provisional application No. 61/315,483, filed on Mar. combination with other histological dyes for nuclear counter 19, 2010. staining. Reticulin Stain Patent Application Publication Sep. 22, 2011 Sheet 1 of 3 US 2011/0229879 A1 Reticulin Stain Figures 1A-1B Patent Application Publication Sep. 22, 2011 Sheet 2 of 3 US 2011/0229879 A1 Iron Stain Figures 2A-2B Patent Application Publication Sep. 22, 2011 Sheet 3 of 3 US 2011/0229879 A1 Alcian Blue Figures 3A-3B US 2011/0229.879 A1 Sep. 22, 2011 METHODS AND COMPOSITIONS FOR composition of the present invention does not precipitate in NUCLEAR STANING Solution and has a shelf-life of at least one year. In addition, nuclear staining with the composition of the present invention achieves brighter, more brilliant nuclear staining showing 0001.
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