Product datasheet Anti-NUP214 Antibody Catalog Number: A02408

BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China

Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected]

Basic Information Product Name Anti-NUP214 Antibody Name NUP214 Source Rabbit IgG Species Reactivity human,mouse Tested Application WB,ICC/IF,FCM,Direct ELISA Contents 500ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA and 50% glycerol. Immunogen E.coli-derived human NUP214 recombinant (Position: K36-E374). Purification Immunogen affinity purified. Observed MW 280KD Dilution Ratios Western blot: 1:500-2000 Immunocytochemistry/Immunofluorescence (ICC/IF): 1:50-400 Flow Cytometry: 1-3μg/1x106 cells Direct ELISA： 1:100-1000

Storage 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing

Background Information 214 (Nup2014) is a protein that in humans is encoded by the NUP214 gene. The complex is a massive structure that extends across the , forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. are the main components of the nuclear pore complex in eukaryotic cells. This gene is a member of the FG-repeat-containing nucleoporins. The protein encoded by this gene is localized to the cytoplasmic face of the nuclear pore complex where it is required for proper progression and nucleocytoplasmic transport. The 3' portion of this gene forms a fusion gene with the DEK gene on 6 in a t(6,9) translocation associated with and . Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.

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FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 1 Product datasheet Anti-NUP214 Antibody Catalog Number: A02408

BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China

Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected]

Selected Validation Data

Figure 1. Western blot Figure 2. Flow Cytometry analysis of NUP214 using analysis of HL-60 cells anti-NUP214 antibody using anti-NUP214 (A02408). antibody (A02408). Overlay histogram Lane 1: human Hela showing HL-60 cells whole cell lysates, stained with A02408 Lane 2: human HEK293 (Blue line).anti-NUP214 whole cell lysates, Antibody (A02408, Lane 3: human K562 1μg/1x106 cells) for 30 whole cell lysates, min at 20°C. DyLight488 Lane 4: human THP-1 conjugated goat anti- whole cell lysates. rabbit IgG (BA1127, anti-NUP214 antigen 5-10μg/1x106 cells) was affinity purified used as secondary polyclonal antibody antibody . Isotype control (Catalog # antibody (Green line) was A02408)probed with a rabbit IgG (1μg/1x106) goat anti-rabbit IgG-HRP used under the same secondary antibody The conditions. Unlabelled signal is developed using sample (Red line) was an Enhanced also used as a control. Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for NUP214 at approximately 280KD. The expected band size for NUP214 is at 214KD.

Figure 3.ICC analysis Figure 4.ICC analysis using anti-NUP214 using anti-NUP214 antibody (A02408) was antibody (A02408) was detected in immersion detected in immersion fixed A431 cell line . Cells fixed NIH3T3 cell line . were stained using the Cells were stained using Dylight488-conjugated the Anti-rabbit IgG Dylight594-conjugated Secondary Antibody Anti-rabbit IgG (green)Catalog?#?BA112 Secondary Antibody 7) and counterstained (red)Catalog?#?BA1142) with DAPI (blue). and counterstained with DAPI (blue).

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 2

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