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Neurotransmission: Receptor Signaling

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Neurotransmission - receptor and channel signaling GPCRs TYROSINE KINASES ION CHANNELS Neurotrophins BDNF NGF NT-3 Glutamate GABA ACh GDNF Neurturin Artemin Persepin NT-4/5 Glutamate Anandamide ATP Glycine γ 2 1 4 3 / α α α 2-AG α β / α A GFR GFR GFR GFR NTR mGLuR A/B/C X1, 2, 3 CB1/2 2 EphA/B* Ephrin RET Trk p75 AMPA/KainateNMDAR P GABA GlyR nAChR IP3 Lyn released Ephexin Fyn Rapsin Homer DAG Adenylate PI3K Grb4 Src PSD95 HAP1 Syntrophin i PD Shc Gephyrin Gi/q cyclase Gi K PLC GABARAP NcK 2RGS3 PI3 Shc + 2+ 2+ GRIF1 PI3K PLC γ Na Ca Ca P PI3K Grb2 PLCβ/γ PKC nNOS γ FAK PiP3/4 SOS MEK CaM Cl- Gephyrin Cl- ER NFkB PDEI PKA Rac RhoA RasGAP Src PTEN PKC Ras GTPase ERK CaMK Cl- 2+ PKK2 Calcineurin Ca ROCK ERK FRS2 AKT/PKB released Raf JNK nNOS SynGAP Grb2 actin MEK 1/2 P Ser133 Inhibition Excitation Src SOS Cell survival CREB of signaling of signaling RAS P CREB ERK 1/2 P P MAPK GSK3 ELK RSK NFκB CREB CREB gene Long term Cytoskeleton transcription synaptic dynamics, neurite ASK1 Bad CRE plasticity (LTP) P P extension CREB CREB Short term MKK3/6 Apoptosis plasticity Others Other GPCRs - ARA9 ACh (Muscarinic): M1, M2, M3, M4 CRE p38MAPK c-fos Potassium - ASICβ, ASIC3 Adrenalin: Alpha1b, 1c, 1d - KChIP2 - DDR 1, 2 ATP: P2Y MSK1 c-jun c-fos gene - KCNQ 3, 5 - ENSA Dopamine: D1, D4 transcription - Kv beta 2 - GJB1 GABA: GABAB receptor - Kv 1.2 - SLC31A1 μ δ κ P P Opioid: , , CREB CREB AP1 - Kv 1.3 - TRAR4 Neurotensin: 1, 2 Growth, - Kv 1.4 - TRPM7 Neurokinin: NKA, NKB, NK1, Calcitonin development & Sodium - Kv 2.2 - VR1 * forward/reverse signalling differentation Serotonin: 5HT-1B, D, E, F, 5HT-2A, B, 5HT-3, 4, 6, 7 inhibitory pathway CRE - APXL - Kv 4.2 - VRL1 www.abcam.com Related antibodies from Abcam: See more products at: www.abcam.com/neuroscience Product Clonality Applications Host Species Datasheet Product Clonality Applications Host Species Datasheet reactivity www.abcam.com/ab… reactivity www.abcam.com/ab… 5HT1A Receptor P ICC/IF, IHC-FoFr, WB Rb Hu, Rat 44635 Metabotropic Glutamate Receptor 1 P IHC-FoFr, IHC-P,IP, WB Rb Hu, Ms, Rat, Chk, Mk 27199 5HT1B Receptor P IHC-P Rb Hu 13154 Metabotropic Glutamate Receptor 1a P IHC-Fr, IP, WB Rb Ms, Rat, Cat 6439 5HT2A Receptor P IHC-FoFr, IHC (PFA fixed), WB Rb Ms, Rat 16028 Metabotropic Glutamate Receptor 3 P ICC, IHC-P Rb Hu 13193 5HT2B Receptor P IHC-Fr, IHC (PFA fixed), WB Rb Hu, Rat 32994 Metabotropic Glutamate Receptor 4 P IHC-P,IP, WB Rb Hu, Ms, Rat, Chk, Mk, Shp 15305 5HT2C Receptor P IHC-FoFr, IHC-Fr, WB Rb Ms, Rat 32172 Metabotropic Glutamate Receptor 5 P ELISA, IHC-P, WB Rb Hu, Ms, Rat 53090 5HT3 Receptor P WB Rb Hu, Rat 13897 Metabotropic Glutamate Receptor 7 P ELISA, IHC-P, WB Rb Hu, Ms, Rat 53705 5HT6 Receptor P ICC/IF, WB Rb Hu, Rat 41052 Metabotropic Glutamate Receptor 8 P ELISA, IHC-P, WB Rb Hu, Ms, Rat 53094 5HT7 Receptor P WB Rb Hu, Ms, Rat 13898 Monoamine Oxidase A P ELISA, WB Gt Hu 40835 alpha 1b Adrenergic Receptor P WB Chk Hu 15851 Monoamine Oxidase B P ELISA, WB Gt Hu 47988 beta 1 Adrenergic Receptor P IF, WB Rb Ms, Rat 3546 Mu Opioid Receptor P ICC, IHC-Fr, WB Rb Hu, Ms, Rat, NHuPrm 10275 Calcium Sensing Receptor P ICC, IHC-P,IP, WB Rb Hu, Rat 3513 Muscarinic Acetylcholine Receptor M3 P IHC-P Rb Hu 13063 Cannabinoid Receptor I P IHC-P, WB Rb Hu, Ms, Rat 23703 Muscarinic Acetylcholine Receptor M3 Cannabinoid Receptor II P IHC-FoFr, WB Rb Hu, Ms, Rat 45942 - Carboxyterminal end P ELISA, ICC, WB Rb Rat 41169 Choline Acetyltransferase P IHC-FoFr, IHC-P, WB Shp Hu, Rat, Gpig, Pig, Rb 18736 Muscarinic Acetylcholine Receptor M5 P ELISA, IHC (PFA fixed), WB Rb Rat 41171 Corticotropin Releasing Factor P AP, ICC, IHC-P, RIA Rb Hu, Ms, Rat 8901 Natriuretic Peptide Receptor B - DARPP32 (phospho T34) P ELISA, WB Rb Hu, Rat 18553 Carboxyterminal end P ELISA, IHC-P, WB Rb Hu, Ms 37620 Dopamine beta Hydroxylase P ICC/IF, IHC-FoFr, IHC-Fr, WB Rb Ms, Rat, Cow 43868 Neurokinin 1 Receptor P IHC-P, IHC (PFA fixed) Rb Hu, Ms, Rat 13133 Dopamine D2 Receptor P ELISA, ICC, IP, WB Rb Rat 21218 Neuropeptide Y P Dot, IHC-FoFr, IHC-Fr, IHC-P, RIA Rb Hu, Ms, Rat, Mk, Rb 10980 Dopamine Receptor D1 P ELISA, ICC, WB Rb Hu, Rat 20066 Nicotinic Acetylcholine Receptor alpha 1 P IHC-P, WB Rb Hu, Ms, Rat, Rb 65832 Dopamine Transporter P ELISA, WB Rb Hu, Ms, Rat 18548 Nicotinic Acetylcholine Receptor alpha 4 P ELISA, ICC/IF, WB Rb Hu, Ms, Rat, Chk, Chmp, Eph receptor B2 P ELISA, IHC-P, WB Rb Hu, Ms 5418 Pfsh, Zfsh 41172 GABA A Receptor alpha 1 P IHC-P, WB Rb Hu, Ms, Rat, Many 65269 Nicotinic Acetylcholine Receptor alpha 7 P ICC/IF, IHC-P, WB Rb Hu, Ms 10096 GABA A Receptor alpha 2 P WB Rb Ms, Rat 8342 NMDAR1 P ELISA, ICC/IF, IP, WB Rb Hu, Ms, Rat 28669 GABA A Receptor alpha 4 P IP, WB Rb Ms, Rat 4120 NMDAR2A (phospho Y1325) P WB Rb Ms 16646 GABA A Receptor alpha 5 P WB Rb Ms, Rat 3700 NMDAR2A P IHC-Fr, IHC-P,IP, WB Rb Ms, Rat, Mk 14596 GABA A Receptor beta 3 P Flow Cyt, ICC, IHC-FoFr, IP, WB Rb Hu, Ms, Rat 4046 NMDAR2B (phospho Y1472) P ELISA, IHC-P Rb Hu, Ms, Rat 59205 GABA A Receptor gamma 2 P IP, WB Rb Ms, Rat 4073 NMDAR2B P IHC-FoFr, IHC-Fr, IP, WB Rb Hu, Ms, Rat 109 GABA A Receptor gamma 2 - nNOS (neuronal) (phospho S847) P IHC-FoFr, WB Rb Ms, Rat 16650 Extracellular domain P IHC-Fr, WB Rb Hu, Ms, Rat, Cow 49961 Noradrenaline transporter P Dot, ELISA, WB Rb Hu, Ms, Rat 41559 GABA P ELISA, ICC/IF, IHC-Glut Rb Rat 43865 Ryanodine Receptor (phospho S2808) P ELISA, IHC-P Rb Hu, Ms 59225 GABA B Receptor 1 P WB Rb Hu, Rat 1135 Serotonin transporter P ICC/IF, IHC-Fr, IHC-P Rb Rat 44520 GABA Receptor Epsilon P WB Rb Hu 35971 Sodium Channel alpha (epithelial) P IHC-Fr, IHC-P, WB Rb Hu 65710 GLP1R P IHC-P Rb Hu, Ms, Rat, Hm 13181 Somatostatin 28 P EM, IF, IHC-Fr, IHC-P Rb Hu, Ms, Rat, Cat, Chk, Cow, GluR2 (phospho S880) P ELISA, IHC-P, WB Rb Hu, Ms, Rat 52180 Dog, Gpig, Pig, Shp 22682 GluR2 P ICC/IF, IHC-P, WB Rb Hu, Ms 40878 Somatostatin P ELISA, WB Rb Hu, Ms, Rat 53165 Glutamate Receptor 1 (AMPA subtype) P WB Rb Ms 31232 Somatostatin Receptor 2 P ICC/IF, IHC-P, WB Rb Hu, Ms, Rat 9550 Glutamate Transporter 1 P WB Rb Ms, Rat 41621 Somatostatin Receptor P IHC-Fr, IHC-P Rb Hu, Ms, Rat 2366 Glutamine Synthetase P IHC-FoFr, IHC-Fr, WB Rb Hu, Ms, Rat 16802 Substance P P IHC-Fr Gpig Hu, Ms, Rat 10353 Kappa Opioid Receptor P ICC, IHC-Fr Rb Hu 10283 Synapsin I - Synaptic Marker P ICC, IHC-FoFr, IHC-Fr, IP, WB Rb Hu, Ms, Rat, Cow, Gpig 8 MC4 Receptor P IHC-P, WB Rb Hu, Ms, Rat 24233 Vasopressin P IHC-P, WB Rb Hu, Ms, Rat, Rb, Shp 8745 Met Enkephalin P IHC-P, IHC-R Rb Hu, Rat, Gpig, Pig 22620 Vesicular Acetylcholine Transporter P IHC-Fr, IHC-P, WB Gt Hu, Ms, Rat 62140 Cannabinoid Receptor II antibody (ab45942) Serotonin transporter antibody (ab44520) Clonality Applications Host Species cross reactivity Clonality Applications Host Species cross reactivity P IHC-FoFr, WB Rb Hu, Ms, Rat P ICC/IF, IHC-Fr, IHC-P Rb Rat Cannabinoids exert their well known physiological effects through two G protein coupled Serotonin (5-hydroxytryptamine; 5-HT) is a neurotransmitter in the central and receptors, cannabinoid receptor 1 (CB1) and CB2. Both cannabinoid receptors have been peripheral nervous systems. Following release, Serotonin is actively cleared shown to inhibit adenylyl cyclase as well as stimulate the mitogen-activated protein kinase, from synaptic spaces by a a high-affinity, Na(+)- and Cl(-)-dependent MAPK. transporter localized in presynaptic neuronal membranes..
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  • Advances in Surgery for Pituitary Tumors

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    roper_subbed.qxp 14/1/09 9:36 am Page 36 Brain Trauma Neuro-oncology Advances in Surgery for Pituitary Tumors a report by Steven N Roper, MD Edward Shedd Wells Professor of Neurosurgery, University of Florida DOI: 10.17925/USN.2008.04.02.36 Although benign in nature, pituitary tumors continue to offer many at birth the anterior pituitary gland is composed of cells that were created opportunities for improvement in therapy. Goals of treatment include by embryonic stem cells. However, throughout post-natal life, adult stem complete removal or ablation of tumor cells, maintenance of normal pituitary cells contributed new pituitary cells of all major sub-types to the gland. This function, normalization of elevated hormone levels in endocrine-active means that the adult gland represents a mosaic of cells that have similar tumors, and minimizing adverse effects from therapy. There have been many phenotypes (i.e. lactotrophs, somatotrophs, etc.) but have very different recent advances related to the surgical treatment of pituitary tumors and the origins (i.e. embryonic or adult progenitor cells). They also provided purpose of this article is to review some of the more significant ones. preliminary evidence that adult stem cells may be involved in tumorigenesis in the pituitary in a mouse model, the retinoblastoma (Rb-1)+/- mouse. This Behavior of Pituitary Tumors will, no doubt, stimulate considerable interest in the study of the cellular One of the most notable features of pituitary adenomas is their ubiquity in originators of human pituitary tumors. population studies compared with the relatively small number of tumors that actually present with clinical problems.
  • Characterisation of L-Cell Secretory Mechanisms and Colonic

    Characterisation of L-Cell Secretory Mechanisms and Colonic

    Characterisation of L -cell secretory mechanisms and colonic enteroendocrine cell subpopulations Lawrence Billing Downing College, Cambridge This dissertation is submitted for the degree of Doctor of Philosophy September 2018 Abstract Enteroendocrine cells (EECs) are chemosensitive cells of the gastrointestinal epithelium that exert a wide range of physiological effects via production and secretion of hormones in response to ingested nutrients, bacterial metabolites and systemic signals. Glucagon-like peptide-1 (GLP-1) is one such hormone secreted from so-called L-cells found in both the small and large intestines. GLP-1 exerts an anorexigenic effect and together with glucose- dependent insulinotropic polypeptide (GIP), restores postprandial normoglycaemia through the incretin effect. These effects are exploited by GLP-1 analogues in the treatment of type 2 diabetes. GLP-1 may also contribute to weight-loss and remission of type 2 diabetes following bariatric surgery which increases postprandial GLP-1 excursions. Here we investigated stimulus secretion coupling in L-cells. A novel 2D culture system from murine small intestinal organoids was established as an in vitro model. This was used to characterise synergistic stimulation of GLP-1 secretion in response to concomitant stimulation by bile acids through the Gs-protein coupled receptor GPBAR1 and free fatty acids through the Gq-coupled receptor FFAR1. Roughly half of colonic, but not small intestinal, L-cells co-produce the orexigenic peptide insulin-like peptide 5 (INSL5). This hitherto poorly examined subpopulation of L-cells was characterised through transcriptomic analysis, intracellular calcium imaging (using a novel GCaMP6F-based transgenic mouse model), LC/MS peptide quantification and 3D super resolution microscopy (3D-SIM).