Neurotransmission - receptor and channel signaling GPCRs TYROSINE KINASES ION CHANNELS Neurotrophins BDNF NGF NT-3 Glutamate GABA ACh GDNF Neurturin Artemin Persepin NT-4/5 Glutamate Anandamide ATP Glycine γ 2 1 4 3 / α α α 2-AG α β / α A GFR GFR GFR GFR NTR mGLuR A/B/C X1, 2, 3 CB1/2 2 EphA/B* Ephrin RET Trk p75 AMPA/KainateNMDAR P GABA GlyR nAChR IP3 Lyn released Ephexin Fyn Rapsin Homer DAG Adenylate PI3K Grb4 Src PSD95 HAP1 Syntrophin i PD Shc Gephyrin Gi/q cyclase Gi K PLC GABARAP NcK 2RGS3 PI3 Shc + 2+ 2+ GRIF1 PI3K PLC γ Na Ca Ca P PI3K Grb2 PLCβ/γ PKC nNOS γ FAK PiP3/4 SOS MEK CaM Cl- Gephyrin Cl- ER NFkB PDEI PKA Rac RhoA RasGAP Src PTEN PKC Ras GTPase ERK CaMK Cl- 2+ PKK2 Calcineurin Ca ROCK ERK FRS2 AKT/PKB released Raf JNK nNOS SynGAP Grb2 actin MEK 1/2 P Ser133 Inhibition Excitation Src SOS Cell survival CREB of signaling of signaling RAS P CREB ERK 1/2 P P MAPK GSK3 ELK RSK NFκB CREB CREB gene Long term Cytoskeleton transcription synaptic dynamics, neurite ASK1 Bad CRE plasticity (LTP) P P extension CREB CREB Short term MKK3/6 Apoptosis plasticity Others Other GPCRs - ARA9 ACh (Muscarinic): M1, M2, M3, M4 CRE p38MAPK c-fos Potassium - ASICβ, ASIC3 Adrenalin: Alpha1b, 1c, 1d - KChIP2 - DDR 1, 2 ATP: P2Y MSK1 c-jun c-fos gene - KCNQ 3, 5 - ENSA Dopamine: D1, D4 transcription - Kv beta 2 - GJB1 GABA: GABAB receptor - Kv 1.2 - SLC31A1 μ δ κ P P Opioid: , , CREB CREB AP1 - Kv 1.3 - TRAR4 Neurotensin: 1, 2 Growth, - Kv 1.4 - TRPM7 Neurokinin: NKA, NKB, NK1, Calcitonin development & Sodium - Kv 2.2 - VR1 * forward/reverse signalling differentation Serotonin: 5HT-1B, D, E, F, 5HT-2A, B, 5HT-3, 4, 6, 7 inhibitory pathway CRE - APXL - Kv 4.2 - VRL1 www.abcam.com Related antibodies from Abcam: See more products at: www.abcam.com/neuroscience Product Clonality Applications Host Species Datasheet Product Clonality Applications Host Species Datasheet reactivity www.abcam.com/ab… reactivity www.abcam.com/ab… 5HT1A Receptor P ICC/IF, IHC-FoFr, WB Rb Hu, Rat 44635 Metabotropic Glutamate Receptor 1 P IHC-FoFr, IHC-P,IP, WB Rb Hu, Ms, Rat, Chk, Mk 27199 5HT1B Receptor P IHC-P Rb Hu 13154 Metabotropic Glutamate Receptor 1a P IHC-Fr, IP, WB Rb Ms, Rat, Cat 6439 5HT2A Receptor P IHC-FoFr, IHC (PFA fixed), WB Rb Ms, Rat 16028 Metabotropic Glutamate Receptor 3 P ICC, IHC-P Rb Hu 13193 5HT2B Receptor P IHC-Fr, IHC (PFA fixed), WB Rb Hu, Rat 32994 Metabotropic Glutamate Receptor 4 P IHC-P,IP, WB Rb Hu, Ms, Rat, Chk, Mk, Shp 15305 5HT2C Receptor P IHC-FoFr, IHC-Fr, WB Rb Ms, Rat 32172 Metabotropic Glutamate Receptor 5 P ELISA, IHC-P, WB Rb Hu, Ms, Rat 53090 5HT3 Receptor P WB Rb Hu, Rat 13897 Metabotropic Glutamate Receptor 7 P ELISA, IHC-P, WB Rb Hu, Ms, Rat 53705 5HT6 Receptor P ICC/IF, WB Rb Hu, Rat 41052 Metabotropic Glutamate Receptor 8 P ELISA, IHC-P, WB Rb Hu, Ms, Rat 53094 5HT7 Receptor P WB Rb Hu, Ms, Rat 13898 Monoamine Oxidase A P ELISA, WB Gt Hu 40835 alpha 1b Adrenergic Receptor P WB Chk Hu 15851 Monoamine Oxidase B P ELISA, WB Gt Hu 47988 beta 1 Adrenergic Receptor P IF, WB Rb Ms, Rat 3546 Mu Opioid Receptor P ICC, IHC-Fr, WB Rb Hu, Ms, Rat, NHuPrm 10275 Calcium Sensing Receptor P ICC, IHC-P,IP, WB Rb Hu, Rat 3513 Muscarinic Acetylcholine Receptor M3 P IHC-P Rb Hu 13063 Cannabinoid Receptor I P IHC-P, WB Rb Hu, Ms, Rat 23703 Muscarinic Acetylcholine Receptor M3 Cannabinoid Receptor II P IHC-FoFr, WB Rb Hu, Ms, Rat 45942 - Carboxyterminal end P ELISA, ICC, WB Rb Rat 41169 Choline Acetyltransferase P IHC-FoFr, IHC-P, WB Shp Hu, Rat, Gpig, Pig, Rb 18736 Muscarinic Acetylcholine Receptor M5 P ELISA, IHC (PFA fixed), WB Rb Rat 41171 Corticotropin Releasing Factor P AP, ICC, IHC-P, RIA Rb Hu, Ms, Rat 8901 Natriuretic Peptide Receptor B - DARPP32 (phospho T34) P ELISA, WB Rb Hu, Rat 18553 Carboxyterminal end P ELISA, IHC-P, WB Rb Hu, Ms 37620 Dopamine beta Hydroxylase P ICC/IF, IHC-FoFr, IHC-Fr, WB Rb Ms, Rat, Cow 43868 Neurokinin 1 Receptor P IHC-P, IHC (PFA fixed) Rb Hu, Ms, Rat 13133 Dopamine D2 Receptor P ELISA, ICC, IP, WB Rb Rat 21218 Neuropeptide Y P Dot, IHC-FoFr, IHC-Fr, IHC-P, RIA Rb Hu, Ms, Rat, Mk, Rb 10980 Dopamine Receptor D1 P ELISA, ICC, WB Rb Hu, Rat 20066 Nicotinic Acetylcholine Receptor alpha 1 P IHC-P, WB Rb Hu, Ms, Rat, Rb 65832 Dopamine Transporter P ELISA, WB Rb Hu, Ms, Rat 18548 Nicotinic Acetylcholine Receptor alpha 4 P ELISA, ICC/IF, WB Rb Hu, Ms, Rat, Chk, Chmp, Eph receptor B2 P ELISA, IHC-P, WB Rb Hu, Ms 5418 Pfsh, Zfsh 41172 GABA A Receptor alpha 1 P IHC-P, WB Rb Hu, Ms, Rat, Many 65269 Nicotinic Acetylcholine Receptor alpha 7 P ICC/IF, IHC-P, WB Rb Hu, Ms 10096 GABA A Receptor alpha 2 P WB Rb Ms, Rat 8342 NMDAR1 P ELISA, ICC/IF, IP, WB Rb Hu, Ms, Rat 28669 GABA A Receptor alpha 4 P IP, WB Rb Ms, Rat 4120 NMDAR2A (phospho Y1325) P WB Rb Ms 16646 GABA A Receptor alpha 5 P WB Rb Ms, Rat 3700 NMDAR2A P IHC-Fr, IHC-P,IP, WB Rb Ms, Rat, Mk 14596 GABA A Receptor beta 3 P Flow Cyt, ICC, IHC-FoFr, IP, WB Rb Hu, Ms, Rat 4046 NMDAR2B (phospho Y1472) P ELISA, IHC-P Rb Hu, Ms, Rat 59205 GABA A Receptor gamma 2 P IP, WB Rb Ms, Rat 4073 NMDAR2B P IHC-FoFr, IHC-Fr, IP, WB Rb Hu, Ms, Rat 109 GABA A Receptor gamma 2 - nNOS (neuronal) (phospho S847) P IHC-FoFr, WB Rb Ms, Rat 16650 Extracellular domain P IHC-Fr, WB Rb Hu, Ms, Rat, Cow 49961 Noradrenaline transporter P Dot, ELISA, WB Rb Hu, Ms, Rat 41559 GABA P ELISA, ICC/IF, IHC-Glut Rb Rat 43865 Ryanodine Receptor (phospho S2808) P ELISA, IHC-P Rb Hu, Ms 59225 GABA B Receptor 1 P WB Rb Hu, Rat 1135 Serotonin transporter P ICC/IF, IHC-Fr, IHC-P Rb Rat 44520 GABA Receptor Epsilon P WB Rb Hu 35971 Sodium Channel alpha (epithelial) P IHC-Fr, IHC-P, WB Rb Hu 65710 GLP1R P IHC-P Rb Hu, Ms, Rat, Hm 13181 Somatostatin 28 P EM, IF, IHC-Fr, IHC-P Rb Hu, Ms, Rat, Cat, Chk, Cow, GluR2 (phospho S880) P ELISA, IHC-P, WB Rb Hu, Ms, Rat 52180 Dog, Gpig, Pig, Shp 22682 GluR2 P ICC/IF, IHC-P, WB Rb Hu, Ms 40878 Somatostatin P ELISA, WB Rb Hu, Ms, Rat 53165 Glutamate Receptor 1 (AMPA subtype) P WB Rb Ms 31232 Somatostatin Receptor 2 P ICC/IF, IHC-P, WB Rb Hu, Ms, Rat 9550 Glutamate Transporter 1 P WB Rb Ms, Rat 41621 Somatostatin Receptor P IHC-Fr, IHC-P Rb Hu, Ms, Rat 2366 Glutamine Synthetase P IHC-FoFr, IHC-Fr, WB Rb Hu, Ms, Rat 16802 Substance P P IHC-Fr Gpig Hu, Ms, Rat 10353 Kappa Opioid Receptor P ICC, IHC-Fr Rb Hu 10283 Synapsin I - Synaptic Marker P ICC, IHC-FoFr, IHC-Fr, IP, WB Rb Hu, Ms, Rat, Cow, Gpig 8 MC4 Receptor P IHC-P, WB Rb Hu, Ms, Rat 24233 Vasopressin P IHC-P, WB Rb Hu, Ms, Rat, Rb, Shp 8745 Met Enkephalin P IHC-P, IHC-R Rb Hu, Rat, Gpig, Pig 22620 Vesicular Acetylcholine Transporter P IHC-Fr, IHC-P, WB Gt Hu, Ms, Rat 62140 Cannabinoid Receptor II antibody (ab45942) Serotonin transporter antibody (ab44520) Clonality Applications Host Species cross reactivity Clonality Applications Host Species cross reactivity P IHC-FoFr, WB Rb Hu, Ms, Rat P ICC/IF, IHC-Fr, IHC-P Rb Rat Cannabinoids exert their well known physiological effects through two G protein coupled Serotonin (5-hydroxytryptamine; 5-HT) is a neurotransmitter in the central and receptors, cannabinoid receptor 1 (CB1) and CB2. Both cannabinoid receptors have been peripheral nervous systems. Following release, Serotonin is actively cleared shown to inhibit adenylyl cyclase as well as stimulate the mitogen-activated protein kinase, from synaptic spaces by a a high-affinity, Na(+)- and Cl(-)-dependent MAPK. transporter localized in presynaptic neuronal membranes..