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The Cotreatment of Landfill Leachate and High-Nitrate RSC Advances View Article Online PAPER View Journal | View Issue The cotreatment of landfill leachate and high- nitrate wastewater using SBRs: evaluation of Cite this: RSC Adv.,2019,9,39572 denitrification performance and microbial analysis† Huaguang Liu, a Xingyu Zhou, *bc Chaoshen Zhanga and Jinsong Zhangabc Resourceful disposal of landfill leachate has always been an intractable worldwide problem. This study was conducted to investigate the feasibility of biologically treating a combined waste stream of landfill leachate and high-concentration nitrate nitrogen (high-nitrate) wastewater. Raw landfill leachate was pretreated using anaerobic fermentation and ammonia stripping to improve biodegradability. The control sequencing batch reactor (SBR, named R0) was fed only with synthetic high-nitrate wastewater with sodium acetate as the carbon source, whereas the other experimental SBR (named R1) was loaded with mixtures containing leachates. Excessive increase in leachate adversely affected the cotreatment, and it was concluded that the landfill leachate volume ratio should never exceed 7.5% of the total wastewater Creative Commons Attribution-NonCommercial 3.0 Unported Licence. (14% of the initial COD) based on further batch experiments. The maximum specific denitrification rate of À À1 À À1 58.05 mg NO3 -N (gVSS h) was attained in R1, while that of 32.32 mg NO3 -N (gVSS h) was obtained in R0. Illumina MiSeq sequencing revealed that adding landfill leachate did not change the fact that Pseudomonas, Thauera, and Pannonibacter dominant in the sodium acetate supported the denitrification systems, but led to the adjustment of their relative abundance. Moreover, the narG, nirK, nirS, and norB denitrifying genes exhibited increased abundance by 138–980% in the cotreated system, Received 1st October 2019 which was confirmed by q-PCR analyses. These findings reveal that the denitrification efficiency of Accepted 11th November 2019 activated sludge in SBR cotreated with landfill leachate and high-nitrate wastewater significantly DOI: 10.1039/c9ra07966a improved, and this may contribute toward the understanding of the molecular mechanisms of biological This article is licensed under a rsc.li/rsc-advances denitrification under the blending treatment of leachate and high-nitrate wastewater. activated carbon adsorption are unable to keep pace with the Open Access Article. Published on 02 December 2019. Downloaded 9/28/2021 10:35:41 PM. 1. Introduction generation rate of landll leachates, which places substantial In China, the increasing amounts of municipal solid waste pressure on sanitary landlls for follow-up treatments. There- (MSW) are putting tremendous pressure on the treatment of fore, it is imperative to nd a reasonable strategy to treat landll landll leachates. According to the survey data from the leachate. Professional Committee of Urban Domestic Refuse Treatment As a cost-effective potential carbon source, blending landll of the China Association of Environmental Protection Industry, leachate with domestic sewage for treatment has become the amount of MSW disposed by the Chinese government a popular research topic. Organic carbon concentration in raw exceeded 191.1 million tons in 2015 (ref. 1) and is expected to wastewater of most municipal wastewater treatment plants reach 266.8 million tons by 2025.2 In China, more than 60% (WWTPs) is very low,3,4 but the denitrication process needs MSW is disposed of in sanitary landlls, which produce large a large number of carbon sources for consumption as electron amounts of landll leachates (Fig. S1†). However, due to the donors. Therefore, to prevent incomplete denitrication and complex structure and high cost of landll leachates, the nitrite nitrogen accumulation caused by insufficient carbon treatment capacity of mainstream advanced technologies such sources, supplementary carbon sources such as methanol, as nanoltration, reverse osmosis, advanced oxidation, and acetic acid, or glucose need to be added to the denitrication tank to ensure that the total nitrogen content in the effluent meets the emission standards.5,6 Moreover, rapidly biodegrad- aSchool of Civil Engineering, Guangzhou University, 510006, Guangzhou, China able forms of organic matter, such as short-chain fatty acids bShenzhen Municipal Wastewater Treatment and Recycling Engineering Laboratory, (SCFAs) and volatile fatty acids (VFAs), contained in landll Shenzhen Water (Group) Co. Ltd., 518031, Guangdong, China. E-mail: leachates can provide a carbon source that can be easily [email protected] c assimilated by microorganisms during the denitri cation School of Environment, Harbin Institute of Technology, 150090, Harbin, China † Electronic supplementary information (ESI) available. See DOI: process. Consequently, the combined treatment of land ll 10.1039/c9ra07966a leachates and domestic sewage in WWTPs has been extensively 39572 | RSC Adv.,2019,9,39572–39581 This journal is © The Royal Society of Chemistry 2019 View Article Online Paper RSC Advances studied around the world.7–10 Yu et al. designed an anaerobic– comprehensively explored, which could provide a technical anoxic–oxic (A2/O) bioreactor system to conduct a eld test on reference for the selection of economical and efficient addi- the combined treatment of domestic wastewater with landll tional carbon sources for the treatment of high-nitrate leachates in the Datangsha WWTP (Guangzhou, China); they wastewater. found that the removal rates of chemical oxygen demand + (COD), ammonia nitrogen (NH4 -N), and total nitrogen (TN) 2. Materials and methods were 82.65%, 92.69%, and 57.10%, respectively, at the optimal volume ratio of 0.2%.11 Ferraz et al. studied the optimal mixing 2.1 Experimental materials ratio for the cotreatment of landll leachate and domestic 2.1.1 Experimental apparatus setup and operation. Two sewage in a Brazilian WWTP using a pilot-scale submerged lab-scale plexiglass cylindrical SBR bioreactors (R0 and R1) were aerobic biolter (SAB). The results showed that the removal designed with an inner diameter of 150 mm, height of 600 mm, rates of biological oxygen demand (BOD), COD, and dissolved and effective volume of 10 L (Fig. 1). These two SBRs were organic carbon (DOC) were 98%, 80%, and 80%, respectively, operated under intermittent loading with a hydraulic residence 12 when the leachate volume ratio was 2%. Landll leachate time (HRT) of 8 h, including a stirring time of 3–5 h, settling cotreatment with sanitary wastewater was investigated at the time of 0.5 h, unloading period of 0.25 h, and loading period of bench scale in an upow anaerobic sludge blanket (UASB), and 0.25 h. the COD removal rate was greater than 70% for leachate volume 2.1.2 Characteristics and pretreatment of landll leach- 13 ratios of up to 5%. A combined system of a long-term lab-scale ates. The landll leachate used as a feed in the experiments was reactor was used for a more comprehensive cotreatment of collected from the Xiaping Municipal Sanitary Landll, Shenz- landll leachate and municipal sewage at volumetric ratios of hen, China. Before being mixed with high-nitrate wastewater in 1%, 3%, and 5%, and the excess sludge could be reduced by 20– the SBR bioreactor, the leachate was pretreated by ammonia 48%, resulting in no inhibitory effect on the denitrication stripping and anaerobic fermentation according to the proce- 14 23 Creative Commons Attribution-NonCommercial 3.0 Unported Licence. process. Three Irish WWTPs were selected for eld testing by dures described by El-Gohary and Kamel. Ammonia stripping Brennan et al., who deemed that the coprocessing of landll was achieved by aeration of the landll leachate for 6 h with À leachate with municipal sewage may be the most sustainable compressed air at a ow rate of 3 L min 1 and pH value of 8.5– 15 leachate treatment solution that is currently available. 11. A sealed glass anaerobic reactor with an effective volume of However, research concerning cotreatment with landll 5 L and inoculated with activated sludge from the primary leachate has been limited to municipal domestic sewage, and sedimentation tank of a sewage treatment plant (Shenzhen, reports regarding the cotreatment of landll leachate and high- China) was used to ferment the landll leachate. The leachate nitrate wastewater are rare. treated by ammonia stripping was injected into the anaerobic Wastewater containing high nitrate concentrations is widely fermentation tank. The leachate was fermented for 24 h at 25 C This article is licensed under a derived from various types of industrial applications, such as aer anaerobic conditions were established by adding nitrogen 16 + fertilizer production, stainless steel manufacturing and sh for 5 min. Aer pretreatment, NH4 -N, suspended solids (SS), 17 18 canning, cellophane nishing, wet lime–gypsum desulfur- and other pollutants in the landll leachate were effectively 19 20 ization production, nuclear applications, and ion exchange degraded, and the biodegradability was improved (Table S1†). Open Access Article. Published on 02 December 2019. Downloaded 9/28/2021 10:35:41 PM. processes.21 When compared with domestic sewage treatment, the denitrication process consumes more carbon sources when a biological process that can completely remove nitrogen is used to treat such high-nitrate wastewater. Fern´andez-Nava et al.22 studied denitrication in a sequencing batch reactor À (SBR) fed with nitrate (2500 mg L 1) using wastewater from a candy factory, residue from a so drink factory, and residue from a dairy plant as the organic carbon sources. Nevertheless, it is still unclear whether landll leachate can be used as an alternative carbon source for the treatment of high-nitrate wastewater or not. The current study aimed to evaluate the behavior of landll leachate when cotreated with high-nitrate wastewater in a lab- scale SBR. To better assess the organic matter and nitrogen removal characteristics, the denitri cation rates of activated Fig. 1 Schematic diagram of the SBRs. The two SBRs were equipped sludge in the treatment of high-nitrate nitrogen at different with a stirrer, dissolved oxygen meter, and pH and temperature meters landll leachate volume ratios were compared.
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