Welcome Address
It is our great pleasure and honour to welcome you in Budapest, Hungary on the occasion of the 20th European Bioenergetics Conference. Bioenergeticists from the whole world – not only from Europe – gather every other year to present the most exciting developments from the molecular and atomic resolution details of the machinery involved in the chemiosmotic conversion of light and chemical energy to the physiology and pathology connected to the energy metabolism of cells, with mitochondria occupying the central stage. This year’s EBEC is organized and hosted by the medical school of Budapest, Semmelweis University – celebrating the 200th birthday of its denominator, Semmelweis Ignác in 2018 and the 250th anniversary of its foundation in 2019 – and co-organized by the Biological Research Centre of the Hungarian Academy of Sciences. The aim of the conference is to promote research in, and interaction between the diverse fields ranging from biophysics to medical sciences, and to encourage students and early career investigators to stay involved and build connections with colleagues and leading experts in bioenergetics. EBEC2018 will be attended by approximately 400 delegates. We are pleased to announce the Peter Mitchell Medal acceptance lecture by Etana Padan from the Hebrew University, Jerusalem, who was awarded this prestigious prize by the Mitchell medal committee formed by previous awardees. With nine plenary and sixteen parallel sessions organized, there will be over 100 lectures and a plenary round table session, the latter focusing on the hot topic of the mitochondrial permeability transition pore. Plenty of time will be allocated for viewing the over 250 posters presented by the delegates. We are grateful to members of the International Advisory Committee of EBEC for their suggestions for speakers and to session chairs for volunteering or accepting our invitation. We are also grateful to our sponsors and supporting organizations for their generous financial contributions, especially to assist the participation of students and young investigator. Last but not least, we are indebted to all of you, who bring the latest and most exciting science to EBEC2018! It only remains for us to wish you a successful conference and a memorable stay in Budapest, the capital city of Hungary.
László Tretter László Zimányi Organizer of EBEC2018 Co-organizer of EBEC2018 Department of Medical Biochemistry Institute of Biophysics Biological Research Centre, Semmelweis University, Budapest Hungarian Academy of Sciences, Szeged
PREVIOUS EUROPEAN BIOENERGETICS CONFERENCES NEXT EUROPEAN BIOENERGETICS CONFERENCE 1980 Urbino, Italy 1982 Lyon, France 2020 Marseille, France 1984 Hannover, Germany 1986 Prague, Czech Republic 1988 Aberystwyth, United Kingdom MITCHELL MEDAL AWARDEES 1990 Noordwijkerout, The Netherlands 1992 Helsinki, Finland 1994 P. Leslie DUTTON 1994 Valencia, Spain 1996 John E. WALKER 1996 Louvain-la-Neuve, Belgium 1998 Wolfgang JUNGE 1998 Göteborg, Sweden 2000 Mårten WIKSTRÖM 2000 Brighton, United Kingdom 2002 Horst WITT 2002 Arcachon, France 2004 Hartmut MICHEL 2004 Pisa, Italy 2006 Masasuke YOSHIDA 2006 Moscow, Russian Federation 2008 David G. NICHOLLS 2008 Dublin, Ireland 2010 Dieter OESTERHELT 2010 Warsaw, Poland 2012 H. Ronald KABACK 2012 Freiburg, Germany 2014 Robert B. GENNIS 2014 Lisbon, Portugal 2016 Peter RICH 2016 Riva del Garda, Italy 2018 Etana PADAN
COMMITTEES AND ORGANISATION Carola HUNTE, Germany Local Organizing Committee Cesare INDIVERI, Italy László TRETTER, László ZIMÁNYI Amandine MARÉCHAL, UK Attila AMBRUS Anthony L. MOORE, UK Christos CHINOPOULOS Plácido NAVAS LLORET, Spain Andras EKE David G. NICHOLLS, USA Beata TOROCSIK Wolfgang NITSCHKE, France Artur OSYCZKA, Poland Scientific Committee Etana PADAN, Israel Paolo BERNARDI, Italy Manuela PEREIRA, Portugal Peter BRZEZINSKI, Sweden Eduardo RIAL, Spain Gary CECCHINI, USA Peter R. RICH, UK Andrei D. VINOGRADOV, Russia Barbara SCHOEPP-COTHENET, France P. Leslie DUTTON, USA Adam SZEWCZYK, Poland Thorsten FRIEDRICH, Germany László TRETTER, Hungary Robert GENNIS, USA Salvador URIBE-CARVAJAL, Mexico Alessandro GIUFFRÈ, Italy John E. WALKER, UK Erich B. GNAIGER, Austria Mårten WIKSTRÖM, Finland Joachim HEBERLE, Germany László ZIMÁNYI, Hungary
INVITED SPEAKERS AND CHAIRS Wayne D. FRASCH USA Pia ÄDELROTH Sweden Christian FREZZA United Kingdom Ulrike ALEXIEV Germany Thorsten FRIEDRICH Germany Susanne ARNOLD The Netherlands Győző GARAB Hungary Peter BAI Hungary José J. GARCIA-TREJO Mexico Robert BALABAN USA Robert B. GENNIS USA Alessandra BARACCA Italy Akos GERENCSER Hungary Paolo BERNARDI Italy Christoph GERLE Japan Giancarlo A. BIAGINI Italy Erich GNAIGER Austria Juan Pedro BOLAÑOS Spain György HAJNOCZKY USA Michael BÖRSCH Germany Joachim HEBERLE Germany Ulrich BRANDT Germany Etienne HEBERT-CHATELAIN Canada Leonid S. BROWN Canada Judy HIRST United Kingdom Peter BRZEZINSKI Sweden Daniel Ken INAOKA Japan Karin B. BUSCH Germany Cesare INDIVERI Italy Susana CADENAS Spain Naotada ISHIHARA Japan Gary CECCHINI USA Petr JEŽEK Czech Republic Quan CHEN China Elizabeth Ann JONAS USA Christos CHINOPOULOS Hungary Frank JORDAN USA Stirling CHURCHMAN USA Ville R.I. KAILA Germany Vito DE PINTO Italy Hideki KANDORI Japan Norbert A. DENCHER Germany Pekka KATAJISTO Finland José FARALDO-GOMEZ USA Yuriy KIRICHOK USA Shelagh FERGUSON-MILLER USA Werner J.H. KOOPMAN The Netherlands David FERRICK USA Andrey V. KOZLOV Austria Giovanni FINAZZI France Werner KUEHLBRANDT Germany
Edmund R.S. KUNJI United Kingdom Alberto SANZ United Kingdom Genji KURISU Japan Leonid SAZANOV Austria Petar H. LAMBREV Hungary Luca SCORRANO Italy Mei LI China Thomas N. SEYFRIED USA Wei LI China Jian-Ren SHEN Japan Michael P. LISANTI United Kingdom Keshav K. SINGH USA William MARTIN Germany Giancarlo SOLAINI Italy Hartmut MICHEL Germany Alexei A. STUCHEBRUKHOV USA Francis MILLET USA Ildikó SZABÓ Italy Anthony L. MOORE United Kingdom Adam SZEWCZYK Poland Jan NEDERGAARD Sweden Marten SZIBOR Finland Nathan NELSON Israel Ye TIAN China Richard NEUTZE Sweden László TRETTER Hungary David G. NICHOLLS USA Salvador URIBE-CARVAJAL Mexico Artur OSYCZKA Poland Rienk VAN GRONDELLE The Netherlands Etana PADAN Israel Andrei VINOGRADOV Russian Federtion Luigi PALMIERI Italy Carlo VISCOMI United Kingdom Manuela PEREIRA Portugal Sir John E. WALKER United Kingdom Nikolaus PFANNER Germany Douglas C. WALLACE USA Peter POHL Austria Mårten WIKSTRÖM Finland Richard PORTER Ireland Richard J. YOULE USA Zsolt RADAK Hungary László ZIMÁNYI Hungary Andrea RASOLA Italy Peter R. RICH United Kingdom Rodrigue ROSSIGNOL France Ann SAADA (REISCH) Israel SCIENTIFIC PROGRAM
SCIENTIFIC PROGRAMME
Saturday 25 August, 2018
16:30 OPENING CEREMONY
17:00 MITCHELL MEDAL PLENARY Chair: Robert B. Gennis
The NhaA Na+/H+ antiporter, new insight into structural and functional dynamics Etana Padan Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem, Israel
18:00 OPENING PLENARY
Chair: László Zimányi
Sixty-five years of the mitochondrial permeability transition: Past, present and future Paolo Bernardi Department of Biomedical Sciences, University of Padova, and Consiglio Nazionale delle Ricerche, Institute of Neuroscience, Padova, Italy
19:00 WELCOME RECEPTION
SCIENTIFIC PROGRAM
Sunday 26 August, 2018
08:20 PLENARY IV.A. MITOCHONDRIA AND CANCER Chair: László Tretter
08:20 Metabolic management of Glioblastoma (30+10’) Thomas N. Seyfried Department of Biology, Boston College, Chestnut Hill, Massachusetts, United States 09:00 PLENARY I. MACROMOLECULAR STRUCTURE AND FUNCTION 1 Chair: Thorsten Friedrich
09:00 Cryo-EM structures of complex I from mouse heart mitochondria in biochemically-defined states (30+10’) Judy Hirst MRC Mitochondrial Biology Unit, University of Cambridge, United Kingdom 09:40 Structure of the alternative complex III in a supercomplex with cytochrome oxidase (30+10’) Robert B. Gennis Department of Biochemistry, University of Illinois, Urbana, USA 10:20 Regulation, functional analysis and assembly of dimeric ATP synthases in mitochondria (30+10’) John E. Walker MRC Mitochondrial Biology Unit, Cambridge, United Kingdom
11:00 Coffee Break
11:30 PLENARY II. MACROMOLECULAR STRUCTURE AND FUNCTION 2 Chair: Peter Pohl
11:30 Cytochrome c oxidase – a molecular machine (30+10’) Mårten Wikström Institute of Biotechnology, University of Helsinki, Finland SCIENTIFIC PROGRAM
12:10 Terminal oxidases of the heme-copper and bd oxidase type, a structural and functional comparison (30+10’) Hartmut Michel Department of Molecular Membrane Biology, Max Planck Institute of Biophysics, Frankfurt am Main, Germany 12:50 CryoEM structures of ATP synthase (30+10’) Werner Kühlbrandt Max Planck Institute of Biophysics, Department of Structural Biology, Frankfurt am Main, Germany
13:30 LUNCH and POSTER VIEWING 1.
ROOM "A" ROOM "B" 15:00 1A. QUINONE REDUCTASES 15:00 1B. OXIDATIVE STRESS Chair: Alexei A. Stuchebrukhov Chair: Andrei Vinogradov
15:00 Tweaking the gearbox: the mechanism and 15:00 Human 2-oxo acid dehydrogenase multienzyme complexes: regulation of mitochondrial complex I (20+5’) experimental observation of the thiamin diphosphate- Ulrich Brandt enamine radical species and its contribution to generation of Radboud University Medical Center, Nijmegen, superoxide and hydrogen peroxide in vitro (20+5’) Netherlands; University of Cologne, Germany Frank Jordan Department of Chemistry, Rutgers University, Newark, New Jersey 07102-1811, USA 15:25 Coupling mechanism of complex I (20+5’) 15:25 Site-specific ROS signalling during ageing (20+5’) Leonid Sazanov Alberto Sanz Institute of Science and Technology Austria, Institute for Cell and Molecular Biosciences, Newcastle University Am Campus 1, Klosterneuburg, 3400 Austria Institute for Ageing, Newcastle University, Newcastle upon Tyne, United Kingdom
SCIENTIFIC PROGRAM
ROOM "A" ROOM "B" 15:50 Molecular mechanism of long-range proton- 15:50 UCP3 regulation in response to oxidative stress and its role electron coupling in respiratory complex I (20+5’) in cardioprotection (20+5’) Ville R.I. Kaila Susana Cadenas Department of Chemistry, Technical University Munich Centro de Biología Molecular “Severo Ochoa” (CSIC/UAM), Madrid, (TUM), Germany Spain
16:15 Large scale atomistic simulations unveil key role 16:15 E3-deficiency by the pathogenic mutations of the human of ubiquinone dynamics in proton pumping by dihydrolipoamide dehydrogenase: elucidation of the mitochondrial complex I (15+5’) molecular pathomechanism by a multifaceted structural Vivek Sharma approach (15+5’) Department of Physics, University of Helsinki, Attila Ambrus Helsinki, Finland MTA-SE Laboratory for Neurobiochemistry, Department of Medical Biochemistry, Semmelweis University, Budapest, Hungary 16:35 Superoxide: quinone oxidoreductase – a new 16:35 Mitochondrial reactive oxygen species production and player in the respiratory chain? (15+5’) pxygen level: linear dependence or not? (15+5’) Christoph von Ballmoos Alexander Galkin Department of Chemistry and Biochemistry, University of Department of Pediatrics, Columbia University, Feil Family Brain and Bern, Switzerland Mind Research Institute, Weill Cornell Medicine, New York, NY, USA
17:00 Coffee Break
SCIENTIFIC PROGRAM
ROOM "A" ROOM "B" 17:30 2A. REGULATION OF RESPIRATORY CHAIN, 17:30 2B. MITOCHONDRIAL PHYSIOLOGY, PATHOLOGY FROM SUBUNITS TO SUPERCOMPLEXES Chair: Rodrigue Rossignol Chair: Gary Cecchini
17:30 The protonmotive force under pressure: an 17:30 Coenzyme Q10 depletion induces endogenous hypoxia in isomorphic analysis (20+5’) cultured cells (15+5') Erich Gnaiger Irene Liparulo D. Swarovski Research Laboratory, Department of Visceral, Department of Pharmacy and Biotechnology - FaBiT, University of Transplant and Thoracic Surgery, Medical University of Bologna, Italy Innsbruck, Austria; Oroboros Instruments, Innsbruck, Austria 17:55 Regulatory interactions within the respiratory 17:50 Knockout of DAPIT protein disrupts ATP synthase chain (20+5’) oligomerisation and has a profound role in regulation of Peter Brzezinski glucose homeostasis (15+5') Department of Biochemistry and Biophysics, The Tomáš Mráček Arrhenius Laboratories for Natural Sciences, Stockholm Institute of Physiology, Czech Academy of Sciences, Prague, University, Stockholm, Sweden Czech Republic 18:20 3D super-resolution microscopy reflects 18:10 Mitochondrial uncoupling protein 3 is a biomarker of mitochondrial cristae alternations and mtDNA heart development and fatty acid β-oxidation nucleoid size and distribution (20+5’) metabolism (15+5') Petr Ježek Elena E. Pohl Department of Mitochondrial Physiology, Institute of Department of Biomedical Sciences, University of Veterinary Physiology of the Czech Academy of Sciences, Prague, Medicine, Vienna, Austria Czech Republic
SCIENTIFIC PROGRAM
ROOM "A" ROOM "B" 18:45 Microcompartmention of mitochondrial OXPHOS, 18:30 Cyclophilin D isomerase activity controls FoF1-ATP resolved with superresolution microscopy (20+5’) synthase oligomerization and mitochondrial Karin B. Busch ultrastructure (15+5') Mitochondrial Dynamics Group, School of Biology, Rubén Quintana-Cabrera University of Osnabrück, Germany Institute of Functional Biology and Genomics (IBFG), Department of Biochemistry and Molecular Biology, University of Salamanca; Institute of Biomedical Research of Salamanca, University Hospital of Salamanca, CIBERFES. Institute of Health Carlos, Madrid, Spain 19:10 Subunit composition of the membrane energy- 18:50 Pore formation by yeast mitochondrial ATP synthase transducing complexes in diverse bacteria and involves subunits e, g and b (15+5') archaea (15+5') Michela Carraro Michael Y. Galperin Department of Biomedical Sciences, University of Padova, Consiglio National Center for Biotechnology Information, NLM, Nazionale delle Ricerche, Institute of Neuroscience, Padova, Italy National Institutes of Health, Bethesda, Maryland, USA 19:30 The atypical subunit composition of oxidative 19:10 A role for mitochondrial uncoupling protein 3 in CD4+ phosphorylation complexes is associated with original T cell function (15+5') extra structural domains in Euglena gracilis (15+5') Emma B. O'Connor Héctor V. Miranda-Astudillo School of Biochemistry and Immunology, Trinity Biomedical Laboratoire de Génétique et Physiologie des Microalgues, Sciences Institute, Trinity College Dublin, Ireland InBioS/Phytosystems, Institut de Botanique, Université de Liège, Belgium 19:50 Control of UCP1 activity in brown-fat 19:30 Tissue- and substrate-specific patterns in the oxygen mitochondria (15+5') kinetics of mitochondrial respiration (15+5') Jan Nedergaard András T. Mészáros The Department of Molecular Biosciences, The Wenner-Gren Oroboros Instruments, Innsbruck, Austria; Institute of Surgical Institute, Stockholm University, Sweden Research, University of Szeged, Hungary
SCIENTIFIC PROGRAM
Monday 27 August, 2018
09:00 PLENARY III. MITOCHONDRIAL STRUCTURE, PHYSIOLOGY AND MOTILITY Chair: Robert S. Balaban
09:00 The cristae modulator OPA1 requires mitochondrial ATP synthase oligomers to safeguard mitochondrial function (30+10’) Luca Scorrano University of Padua, Italy 09:40 Mitochondrial calcium signaling (30+10’) György Hajnóczky MitoCare Center, Thomas Jefferson University, USA 10:20 Dynamics of mitochondrial double membranes, genomes, and functions (30+10’) Naotada Ishihara Department of Biological Science, Graduate School of Science, Osaka University and Department of Protein Biochemistry, Institute of Life Science, Kurume University, Japan 11:00 Coffee Break
11:30 PLENARY IV. MITOCHONDRIA AND CANCER Chair: György Hajnóczky
11:30 A novel mitochondrial inhibitor for eradicating breast cancer stem cells (30+10’) Béla Ozsvari, M. Lisanti 12:10 Mitochondrial dysfunction and cancer: metabolites and beyond (30+10’) Christian Frezza MRC Cancer Unit, University of Cambridge, Hutchison/MRC Research Centre, Cambridge, United Kingdom 12:50 Targeting mitochondrial Hexokinase 2 to develop a novel anti-neoplastic approach (30+10’) Andrea Rasola Department of Biomedical Sciences, University of Padova, Italy
SCIENTIFIC PROGRAM
13:30 LUNCH and POSTER VIEWING 2.
14:15-14:45 LUNCHTIME SPECIAL - BARRY BOCHNER'S LECTURE New mitochondrial function assay technology Barry R. Bochner Biolog, Inc., Hayward, CA, USA
ROOM "A" ROOM "B" 15:00 3A. QUINOL OXIDASES AND TERMINAL OXIDASES 15:00 3B. FRONTIERS IN MITOCHONDRIAL RESEARCH Chair: Francis Millet Chair: Paolo Bernardi
15:00 Insights into radical intermediates of catalytic Qo and 15:00 Selective segregation of mitochondria by stem cells Qi sites of cytochrome bc1 (20+5’) Pekka A. Katajisto (20+10’) Artur Osyczka, Marcin Sarewicz University of Helsinki, and Karolinska Institutet, Helsinki, Finland Department of Molecular Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland 15:25 The binding mode of the natural compound ilicicolin H 15:30 Critical role of mitophagy in inflammasome activation and to the Qi site of the mitochondrial cytochrome bc1 hepatocarcinogenesis (20+10’) complex (15+5’) Quan Chen Wei-Chun Kao The State Key Laboratory of Membrane Biology, Chinese Institute for Biochemistry and Molecular Biology, ZBMZ, Faculty Academy of Sciences, Beijing 100101, China of Medicine, University of Freiburg, Germany
SCIENTIFIC PROGRAM
ROOM "A" ROOM "B" 15:45 Structural basis for energy transduction by respiratory 16:00 Co-regulation of mitochondrial and cytosolic translation alternative complex III (20+5’) programs (20+10’) Manuela M. Pereira Stirling Churchman Instituto de Tecnologia Química e Biológica – António Xavier, Department of Genetics, Harvard Medical School, Boston, MA USA Universidade Nova de Lisboa; Departamento de Química e Bioquímica, Faculdade de Ciências, Universidade de Lisboa, Portugal 16:10 Cytochrome bd in mycobacteria (15+5’) 16:30 Mitochondria and Adaptations in Hibernation (20+10’) Dirk Bald Wei Li Department of Molecular Cell Biology, AIMMS, Vrije Retinal Neurobiology Section National Eye Institute, National Universiteit Amsterdam, The Netherlands Institutes of Health Bethesda, MD, USA
16:30 Supercomplexes assembly factor 1 (SCAF1) shapes 17:00 Wnt signaling mediates intercellular mitochondrial metabolism (15+5') unfolded protein response (20+10’) Sara Cogliati Ye Tian Centro Nacional de Investigaciones Cardiovasculares Carlos III State Key Laboratory of Molecular Developmental Biology, (CNIC), Melchor Fernandez Almagro, Madrid, Spain Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China 17:30 Streaming biology in real time to inform adoptive T cell engineering designs (20+5’) David Ferrick Agilent Technologies, Seahorse Bioscience, USA
19:00 ORGAN CONCERT IN THE SZENT ISTVÁN BASILICA
SCIENTIFIC PROGRAM
Tuesday 28 A ugust, 2018
09:00 PLENARY V. TRANSPORT AND METABOLISM Chair: Cesare Indiveri
09:00 A chloroplast-localized mitochondrial calcium uniporter homolog mediates stress-specific response in Arabidopsis plants (30+10’) Ildiko Szabo Department of Biology, University of Padova, Italy 09:40 Tryptophan rich sensory protein, TSPO: Functional insights from high resolution structures (30+10’) Shelagh Ferguson-Miller Michigan State University, Michigan, USA 10:20 Mitochondrial uncoupling proteins: how many of them? (30+10’) Yuriy Kirichok Department of Physiology, University of California San Francisco, CA, USA 11:00 Coffee Break
11:30 PLENARY VI. MITOCHONDRIAL PHYSIOLOGY AND SIGNALIZATION Chair: Richard Porter
11:30 Control of brain metabolism and behavior by cell-type specific mitochondrial CB1 receptors (30+10’) Etienne Hebert-Chatelain Université de Moncton, Department of Biology, Canada 12:10 The mitochondria reticulum of muscle cells (30+10’) Robert S. Balaban Laboratory of Cardiac Energetics, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD, USA 12:40 Roles of endogenous PINK1 and Parkin in vivo – a different type of mitochondrial QC (30+10’) Richard J. Youle NIH National Institute for neurological Disorders and Stroke (NINDS) SCIENTIFIC PROGRAM
13:30 LUNCH AND POSTER VIEWING 3. 13:30 EBEC SCIENTIFIC COMMITTEE MEETING
ROOM "A" ROOM "B" 15:00 4A. TERMINAL OXIDASES 15:00 4B. BIOENERGETICS OF CNS DISEASES Chair: Pia Ädelroth Chair: Norbert A. Dencher
15:00 Subunit structure and regulation of cytochrome 15:00 OXPHOS organization in neurons and astrocytes c oxidase (20+5’) regulates brain metabolism (20+5’) Susanne Arnold Juan P. Bolaños Radboud Institute for Molecular Life Sciences, Radboud Institute of Functional Biology and Genomics (IBFG), University University Medical Center, Nijmegen, The Netherlands of Salamanca, CSIC, Salamanca, Spain 15:25 Cytochrome c oxidase subunit 4 isoform switch 15:25 Thiamine preserves mitochondrial function in a rat model results in modulation of oxygen affinity (15+5’) of traumatic brain injury, preventing inactivation of the Petr Pecina 2-oxoglutarate dehydrogenase complex (20+5’) IPHYS CAS, Department of Bioenergetics, Prague, Andrey V. Kozlov Czech Republic Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Vienna, Austria 15:45 A common core coupling mechanism for 15:50 Inosine reverses motor neuron toxicity observed in mitochondrial and bacterial A-type cytochrome amyotrophic lateral sclerosis patient astrocytes with an c oxidases? (20+5’) adenosine deaminase deficiency (15+5’) Peter R. Rich Scott P. Allen Institute of Structural and Molecular Biology, University Sheffield Institute for Translational Neuroscience (SITraN), College London, United Kingdom University of Sheffield, Sheffield, United Kingdom 16:10 Heme-copper oxidases – mechanisms for chemistry 16:10 A novel model of Leber’s hereditary optic neuropathy: and energy conservation, including proton respiratory insights and effects of idebenone (15+5’) pumping (15+5’) David A. Brown Margareta R.A. Blomberg Virginia Tech, Department of Human Nutrition, Foods, and Department of Organic Chemistry, Arrhenius Laboratories, Exercise and the Virginia Tech Center for Drug Discovery, Stockholm University, Sweden Blacksburg, VA, USA SCIENTIFIC PROGRAM
ROOM "A" ROOM "B" 16:30 Snapshot of an oxygen Intermediate in the catalytic 16:30 Alterations in the circadian clock-dependent reaction of cytochrome c oxidase (15+5’) mitochondrial functions in Parkinson patient fibroblasts Denis L. Rousseau carrying mutated PARK2 (15+5’) Department of Physiology and Biophysics, Albert Einstein Consiglia Pacelli College of Medicine, Bronx, NY, USA Department of Clinical and Experimental Medicine, University of Foggia, Italy
17:00 Coffee Break
17:30 5A. ATP SYNTHASES AND ATPases 17:30 5B. TARGETING OF BIOENERGETIC ORGANELLES FOR Chair: Salvador Uribe-Carvajal MITOCHONDRIAL DISEASES Chair: Anthony L. Moore
17:30 Monitoring single FoF1-ATP synthases at work in 17:30 Anthony L. Moore (opening) (10’) the ABELtrap (20+5’) Michael Börsch Single-Molecule Microscopy Group, Jena University Hospital, Germany 17:55 The uniqueness of subunit α, γ and ε of mycobacterial 17:40 Targeting the mitochondrial electron transport chain F-ATP synthases: Evolutionary variants for niche of Plasmodium falciparum: Opportunities and adaptation (15+5’) challenges towards the development of improved Gerhard Grüber antimalarials for the elimination era (20+5’) School of Biological Sciences, Nanyang Technological Giancarlo A. Biagini University, Singapore Research centre for Drugs and Diagnostics, Liverpool School of Tropical Medicine, Pembroke Place, Liverpool, United Kingdom SCIENTIFIC PROGRAM
ROOM "A" ROOM "B"
18:15 Unidirectional control of the F1FO-ATPase/synthase 18:05 Targeting the alternative oxidase for antitrypanosomal nanomotor by the ζ pawl-ratchet inhibitor protein drug development (20+5’) of Paracoccus denitrificans (20+5’) Daniel Ken Inaoka José J. García-Trejo School of Tropical Medicine and Global Health, Nagasaki Universidad Nacional Autónoma de México (U.N.A.M.), University, 1-12-4, Sakamoto, Nagasaki; Department of Ciudad de México, Mexico Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo; Department of Host-Defense Biochemistry, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Japan 18:40 Zooming in on the F1-ATPase substeps using a theory 18:30 Bioenergetic consequences of xenotopic expression of of molecular transfer (15+5’) Ciona intestinalis alternative oxidase (AOX) in the Sándor Volkán-Kacsó mouse (20+5’) California Institute of Technology, Noyes Laboratory, Marten Szibor Pasadena, CA, USA Faculty of Medicine and Life Sciences, University of Tampere; Institute of Biotechnology, University of Helsinki, Finland
19:00 F1-ATPase dwell and power stroke 18:55 Mitochondrial diseases: from mechanisms to relationships (20+5’) therapies (20+5’) Wayne D. Frasch Carlo Viscomi Arizona State University, Tempe, AZ, USA MRC Mitochondrial Biology Unit, University of Cambridge, United Kingdom 19:20 General discussion (10’)
21:00 GALA DINNER ON THE DANUBE BOAT "EURÓPA"
SCIENTIFIC PROGRAM
Wednesday 29 August, 2018
09:00 PLENARY VII. EVOLUTION AND BIOGENESIS OF BIOENERGETIC SYSTEMS Chair: David G. Nicholls
09:00 Mitochondrial DNA variation in human radiation and disease (30+10’) Douglas C. Wallace Center for Mitochondrial and Epigenomic Medicine, The Children’s Hospital of Philadelphia and Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, USA; Douglas C. Wallace Institute of Mitochondrial and Epigenomic Information Sciences, Xi’an Jiatong University, Xi’an, China 09:40 Mitochondrial machineries for import and assembly of proteins (30+10’) Nikolaus Pfanner Institute of Biochemistry and Molecular Biology University of Freiburg, Germany 10:20 The physiology of microbe number one (30+10’) William Martin Institute of Molecular Evolution Heinrich-Heine-Universitaet Duesseldorf, Germany 11:00 Coffee Break
ROOM "A" ROOM "B" 11:30 6A. MICROBIAL RHODOPSINS: PUMPS AND 11:30 6B. BIOENERGETICS: DISEASE AND HEALTH CHANNELS Chair: Susanne Arnold Chair: László Zimányi
11:30 Interplay of chloride and proton transfers in the novel 11:30 The pathomechanism of COX deficiency includes nuclear class of light-driven anion transporters (20+5’) DNA damage and replicative stress (20+5’) Leonid S. Brown Ann Saada (Reisch) Department of Physics and Biophysics Interdepartmental Metabolic and Enzyme Laboratory, Department of Genetic and Group, University of Guelph, Ontario, Canada Metabolic Diseases; Hadassah Medical Center and the Hebrew University of Jerusalem, Israel SCIENTIFIC PROGRAM
ROOM "A" ROOM "B" 11:55 Molecular mechanism of channelrhodopsin (20+5’) 11:55 Exogenous NAD+ prevents galactose-induced death of Joachim Heberle Leigh Syndrome patient fibroblasts with isolated Freie Universität Berlin, Department of Physics, Experimental complex I deficiency (20+5’) Molecular Biophysics, Berling, Germany Werner J.H. Koopman 12:20 Light-driven sodium-pumping rhodopsin: A new Department of Biochemistry, Radboud Institute for Molecular Life concept of active transport (20+5’) Sciences, Radboud Center for Mitochondrial Medicine, Hideki Kandori Radboudumc, Nijmegen, The Netherlands Nagoya Institute of Technology, Japan 12:20 Targeting the mitochondrial trifunctional protein in 12:45 Electrophysiology of the engineered light-driven oxidative lung carcinomas (20+5’) sodium pump eKR2 and its conversion into a Rodrigue Rossignol channel (15+5’) INSERM U1211, Bordeaux; Bordeaux University; CELLOMET, Arend Vogt Functional Genomics Center (CGFB), Bordeaux, France Experimental Biophysics, Humboldt-Universität zu Berlin, 12:45 Exercise-induced mitochondrial biogenesis: is it a key for Germany diseases prevention? (20+5’) 13:05 Time-resolved serial femtosecond crystallography Zsolt Radák studies at an X-ray free electron laser reveals structural University of Physical Education, Budapest, Hungary changes in bacteriorhodopsin (20+5’) 13:10 The control of oxidative phosphorylation in healthy and Richard Neutze type 2 diabetic human pancreatic β-Cells (20+5’) University of Gothenburg, Sweden Akos A. Gerencser Buck Institute for Research on Aging, Novato, CA, USA
13:30 LUNCH and POSTER VIEWING 4.
SCIENTIFIC PROGRAM
15:00 PTP ROUND TABLE Moderator: Christos Chinopoulos 15:00 John E. Walker (10’) 15:10 Paolo Bernardi (10’) 15:20 ATP synthase in neuronal development, neurodegeneration and plasticity (10’) Elizabeth Ann Jonas Yale University Department Internal Medicine (Endocrinology); New Haven, USA 15:30 Is F-ATP synthase the mitochondrial permeability transition pore? (10’) Christoph Gerle Osaka University, Japan
15:40 Discussion
17:00 Coffee Break
ROOM "A" ROOM "B" 17:30 7A. CHANNELS AND TRANSPORTERS 17:30 7B. BIOENERGETICS OF CANCER Chair: Yuriy Kirichok Chair: Giancarlo Solaini
17:30 The Ins and OUTs of mitochondrial glutamate 17:30 How do cancer cells harness energy? (20+5’) transport (20+5’) Christos Chinopoulos Luigi Palmieri Department of Medical Biochemistry Semmelweis University, Department of Biosciences, Biotechnologies and Budapest, Hungary Biopharmaticeutics University of Bari, Italy 17:55 The DOs and DON’Ts of secondary-active transporters: 17:55 Metabolic vulnerabilities in solid tumors predicted by lessons from the Na+/Ca2+ exchanger (20+5’) rapid ex vivo functional analysis (15+5’) José D. Faraldo-Gómez Andre Koit Theoretical Molecular Biophysics Laboratory National Institute of Chemical Physics and Biophysics, Tallinn, National Heart, Lung & Blood Institute, National Institutes of Estonia Health, Bethesda, MD 20892, USA SCIENTIFIC PROGRAM
ROOM "A" ROOM "B"
18:20 VDAC isoforms in S. cerevisiae (20+5’) 18:15 Zebrafish (Danio rerio) as a model to study the Vito De Pinto pathophysiological role of the mitochondrial chaperone Department of Biomedicine and Biotechnology, TRAP1 (15+5’) University of Catania, Italy Claudio Laquatra Department of Biomedical Sciences and CNR Institute of Neurosciences, University of Padua, Italy
18:45 Mitochondrial regulation of mitochondrial 18:35 The ATP synthase inhibitor protein IF1 plays a significant potassium channels (20+5’) role in cancer metabolic flexibility (20+5’) Adam Szewczyk Alessandra Baracca Laboratory of Intracellular Ion Channels, Nencki Institute of Department of Biomedical and Neuromotor Sciences, Lab of Experimental Biology, Warsaw, Poland Biochemistry and Mitochondrial Pathophysiology, University of Bologna, Italy 19:10 The structural mechanism of transport by the 19:00 Substrate oxidation differences in human glioma cells mitochondrial ADP/ATP carrier (20+5’) and their potential clinical significance (15+5’) Edmund R.S. Kunji Anna Sebestyén MRC Mitochondrial Biology Unit, University of Cambridge, 1st Department of Pathology and Experimental Cancer Research, Cambridge Biomedical Campus, United Kingdom Semmelweis University, Budapest, Hungary 19:35 The human SLC1A5 amino acid transporter: structure/function relationships, regulatory aspects and involvement in energy metabolism (15+5’) Mariafrancesca Scalise Department DiBEST (Biologia, Ecologia, Scienze della Terra) Unit of Biochemistry and Molecular Biotechnology, University of Calabria, Arcavacata di Rende, Italy
SCIENTIFIC PROGRAM
T h u r s d a y 30 August, 2018
ROOM "A" ROOM "B" 09:00 8A. ANTENNAE AND PHOTOSYSTEMS 09:00 8B. VARIABILITY OF MITOCHONDRIAL FUNCTIONS Chair: Ulrike Alexiev AND PATHOLOGIES Chair: Jan Nedergaard
09:00 Cryo-EM structure of maize PSI-LHCI-LHCII 09:00 Sirtuins, NAD+ and mitochondrial bioenergetics: a supercomplex (20+5’) critique (20+5’) Mei Li David G. Nicholls National Laboratory of Biomacromolecules, CAS Center for Buck Institute for Research on Aging, Novato, CA, USA Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China 09:25 Structure and function of photosystem I complexes 09:25 NAD+ homeostasis plays role in mitochondrial biogenesis and potential implications on photosynthetic during beige adipocyte differentiation (20+5’) electron transport regulation in microalgae (15+5’) Péter Bai Michael Hippler Departments of Medical Chemistry; MTA-DE Lendület Institute of Plant Biology and Biotechnology, University of Laboratory of Cellular Metabolism, Debrecen, Hungary; Münster, Germany Research Center for Molecular Medicine, Faculty of Medicine, University of Debrecen, Hungary 09:45 X-ray and NMR studies of the complex between 09:50 Interaction of Alzheimer’s disease triggering amyloid ferredoxin and photosystem I (20+5’) beta peptides with membranes and organelles: Genji Kurisu bioenergetical consequences (20+5’) Institute for Protein Research, Osaka University, Japan Norbert A. Dencher Moscow Institute of Physics and Technology, MIPT, Dolgoprudniy/Moscow Oblast, Russia; Chemistry/Physical Biochemistry, Technical University Darmstadt, Germany SCIENTIFIC PROGRAM
ROOM "A" ROOM "B"
10:10 Energy transfer in LHCII resolved by 2D electronic 10:15 Mitochondrial cAMP augments Ca2+ uptake into the spectroscopy at ambient and low temperatures (20+5’) organelle to support steroidogenesis (15+5’) Petar H. Lambrev Gergő Szanda Institute of Plant Biology, Biological Research Centre, Department of Physiology, Semmelweis University, MTA-SE Hungarian Academy of Sciences, Szeged, Hungary Laboratory of Molecular Physiology, Semmelweis University and Hungarian Academy of Sciences, Budapest, Hungary 10:35 Global spectroscopic analysis to study the regulation 10:35 Mitochondrial patch clamp of beige adipocytes reveals of the proton motive force in photosynthetic UCP1-Positive and UCP1-negative cells both exhibiting organisms (20+5’) futile creatine cycling (15+5’) Giovanni Finazzi Ambre M. Bertholet Cell and Plant Physiology laboratory, CEA Grenoble, France Department of Physiology, University of California, San Francisco, CA, USA 11:00 Coffee Break
11:30 PLENARY VIII. PHOTOSYNTHETIC SYSTEMS Chair: Győző Garab
11:30 The quantum design of photosynthesis (30+10’) Rienk van Grondelle Department of Biophysics, Faculty of Sciences, VU University Amsterdam, De Boelelaan Amsterdam, The Netherlands 12:10 Photosynthetic reaction centers – Robustness with increased complexity (30+10’) Nathan Nelson Department of Biochemistry and Molecular Biology, The George S. Wise Faculty of Life Sciences, Tel Aviv University, Israel
12:50 Mechanism of photosynthetic water-splitting catalyzed by the Mn4CaO5 metal cluster in photosystem II (30+10’) Jian-Ren Shen Research Institute for Interdisciplinary Science, Okayama University, Japan
13:30 CLOSING CEREMONY SCIENTIFIC PROGRAM
POSTERS
P1 a/1 Accessory LYR subunit LYRM6/NDUFA6 has a critical function for complex I activity Heike Angerer Goethe University Frankfurt, Medical School, Institute of Biochemistry II, Structural Bioenergetics Group, Frankfurt, Germany P1 a/2 Molecular simulations of quinone binding into respiratory complex I and of substrate reactivity in complex II Guilherme Menegon Arantes Department of Biochemistry, Universidade de Sao Paulo, São Paulo, Brazil
P1 a/3 Functional characterisation of E. coli cytochrome b561 proteins: A membrane-associated superoxide scavenger? Olivier Biner Department of Chemistry and Biochemistry, University of Bern, Switzerland P1 a/4 Reversible decoupling of the proton pumps of mitochondrial complex I by fixing a loop in the ubiquinone reduction pocket Alfredo Cabrera-Orefice Radboud Institute for Molecular Life Sciences, Department of Pediatrics, Radboud University Medical Center, Nijmegen, The Netherlands; Cluster of Excellence Macromolecular Complexes, Goethe-University, Frankfurt am Main, Germany P1 a/5 Cardiolipin affects respiratory complex I structure and dynamics Andrea Di Luca Department of Chemistry, Technical University of Munich, Germany P1 a/6 A highly conserved loop in the PSST subunit of complex I is essential for ubiquinone binding and reduction Etienne Galemou Yoga Structural Bioenergetics Group, Institute of Biochemistry II, Medical School, Goethe-University Frankfurt am Main, Germany P1 a/7 The mechanism of mammalian respiratory complex I Domen Kampjut Institute of Science and Technology Austria, Am Campus, Klosterneuburg, Austria
SCIENTIFIC PROGRAM
P1 a/8 Probing the conformational heterogeneity of complex I by combination of cryo-electron microscopy, crystallography and normal mode analysis Karol Kaszuba IST Austria, Klosterneuburg, Austria P1 a/9 Proton pumping mechanism of complex I Ievgen Mazurenko School of Biomedical Sciences, University of Leeds, United Kingdom P1 a/10 Characterization of the Quinone-binding pocket of mitochondrial respiratory complex I through specific chemical modifications Masatoshi Murai Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Japan P1 a/11 Proton translocation pathways and inter-subunit coupling in the membrane domain of respiratory complex I Max E. Mühlbauer Department of Chemistry, Technical University Munich, Germany P1 a/12 Assembly of the Iron-Sulfur clusters of complex I in Escherichia coli Franziska Nuber Institute of Biochemistry, Albert-Ludwigs-University, Freiburg, Germany P1 a/13 Reduction of 2-methoxy-1,4-naphtoquinone by mitochondrially-localized Nqo1 yielding NAD+ supports substrate-level phosphorylation during respiratory inhibition Dora Ravasz Department of Medical Biochemistry, Semmelweis University, Budapest, Hungary P1 a/14 Energetics and dynamics of long-range electron transfer in respiratory complex I Michael Röpke Department of Chemistry, Technical University of Munich, Garching, Germany P1 a/15 Energetics and dynamics of proton coupled-electron transfer reactions in the NADH/FMN site of respiratory complex I Patricia Saura Department of Chemistry, Technical University of Munich, Garching, Germany SCIENTIFIC PROGRAM
P1 a/16 Large scale atomistic simulations unveil key role of ubiquinone dynamics in proton pumping by mitochondrial complex I Outi haapanen Department of Physics, University of Helsinki, Institute of Biotechnology, University of Helsinki, Finland P1 a/17 Two-electron reduction of ubiquinone in respiratory complex I Alexei A. Stuchebrukhov Department of Chemistry, University of California Davis, One Shields Avenue, Davis, California, USA P1 a/18 NAD+ Binding site-independent energy-linked reverse electron transfer in respiratory complex I Andrei D. Vinogradov Department of Biochemistry, School of Biology, Moscow State University, Russian Federation P1 a/19 Using molecular approaches to understand complex I deficiency in the Ndufs4 knockout mouse model ZhanYin MRC Mitochondrial Biology Unit, University of Cambridge, United Kingdom P1 a/20 The cryo EM structure of respiratory complex I from Yarrowia lipolytica Volker Zickermann Goethe University Frankfurt, Medical School, Institute of Biochemistry II, Frankfurt am Main, Cluster of Excellence Macromolecular Complexes, Goethe University Frankfurt, Germany P1 a/21 A humanised model of complex I as a tool for mitochondrial disease characterisation Daniel N. Grba MRC Mitochondrial Biology Unit, University of Cambridge, United Kingdom
P1 b/1 N-Acetylcysteine induced S-glutathionylation prevented oxidative stress, bioenergetic and dynamic mitochondrial alterations on kidney insufficiency generate by folic acid administration Omar Emiliano Aparicio-Trejo Department of Biology, Faculty of Chemistry, National Autonomous University of Mexico (UNAM), Mexico P1 b/2 Fluorescence lifetime imaging as a tool to detect oxidative stress and associated effects of drug binding to cytochrome c oxidase Ulrike Alexiev Physics Department, Freie Universität Berlin, Germany SCIENTIFIC PROGRAM
P1 b/3 Dietary supplementation with MicroActive Coenzyme Q10 increases expression of antioxidant genes in Thoroughbred skeletal muscle Caitriona E. Curley School of Biochemistry and Immunology, Trinity Biomedical Sciences Institute (TBSI), Trinity College Dublin, Ireland P1 b/4 The impact of mitochondria and reactive oxygen species on the proliferation of human amniotic mesenchymal stromal cells Sergiu Dumitrescu Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Vienna, Austria P1 b/5 Cytochrome c-mediated peroxidative permeabilization of cardiolipin-containing liposomes is prevented by minocycline Alexander M. Firsov Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Russian Federation P1 b/6 Phospholipase A2γ ablation is associated with decreased expression of selected antioxidant enzymes in liver, heart and brain Martin Jaburek Department of Mitochondrial Physiology, Institute of Physiology of the Czech Academy of Sciences, Prague, Czech Republic P1 b/7 Mitochondrial energetic status and oxidative stress during tardigrade anhydrobiosis Andonis Karachitos Department of Bioenergetics, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, Poznań, Poland P1 b/8 VIRTUAL MITOCHONDRION: A model of ROS production by complex II and energy metabolism Jean-Pierre Mazat IBGC-CNRS & Université de Bordeaux, France P1 b/9 Regulatory role of heme oxygenase and nitric oxide synthase in macrophages Andrea Müllebner Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Vienna, Austria
SCIENTIFIC PROGRAM
P1 b/10 Implication of mitochondrial Reactive Oxygen Species production in cardiomyocyte signaling and cardiac rhythm Audrey Sémont IHU Liryc, Electrophysiology and Heart Modeling Institute, fondation Bordeaux Université, Univ. Bordeaux, Centre de recherche Cardio-Thoracique de Bordeaux, INSERM, Centre de recherche Cardio-Thoracique de Bordeaux, France P1 b/11 The vesicle trap. How to measure the real activity of the phagocyte NADPH oxidase? XavierSerfaty Laboratoire de Chimie Physique, UMR 8000, Paris Sud University, CNRS, Paris Saclay University, Orsay, France P1 b/12 Enhanced ROS production in mitochondria from prematurely aging mtDNA mutator mice Irina G. Shabalina Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, Sweden P1 b/13 Preservation of mitochondrial bioenergetics with CAT-4001 after peroxide injury Alexander H. Thomson Virginia Tech, Department of Human Nutrition, Foods, and Exercise and the Virginia Tech Center for Drug Discovery, Blacksburg, USA P1 b/14 The interplay between mitochondrial reactive oxygen species formation and ubiquinone reduction level in Acanthamoeba castellanii mitochondria Karolina Dominiak Department of Bioenergetics, Adam Mickiewicz University, Poznan, Poland
P2 a/1 Acanthamoeba castellanii UCP protein expressed in yeast system; influence on viability of SOD1- and SOD2-deficient yeast under oxidative stress Nina Antos-Krzeminska Department of Bioenergetics, Faculty of Biology, Adam Mickiewicz University, Poznan, Poland P2 a/2 Acclimation of Danio Renio (zebrafish) embryos to the cold Clarissa S. Barthem Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Federative Republic of Brazil
SCIENTIFIC PROGRAM
P2 a/3 Molecular identity and regulatory mechanisms of the mitochondrial uncoupling protein of regular somatic tissues Ambre M. Bertholet Department of Physiology, University of California, San Francisco, USA P2 a/4 Complete knock-out of both COX4 isoforms in HEK293 cells leads to combined complex IV and complex I deficiency Kristýna Čunátová IPHYS CAS, Department of Bioenergetics, Prague, Czech Republic P2 a/5 Mitochondrial supercomplexes do not enhance catalysis by quinone channeling Justin G. Fedor Mitochondrial Biology Unit, University of Cambridge, United Kingdom P2 a/6 Isolation of the yeast respiratory supercomplex using styrene-maleic acid Andrew M. Hartley Institute of Structural and Molecular Biology, University College London, United Kingdom P2 a/7 The well-known biocide triclosan demonstrates unusually high protonophoric activity on artificial planar BLM and bacterial cell membranes, but moderate uncoupling potency in isolated mitochondria and neuronal cells Elena A. Kotova Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russian Federation P2 a/8 The impact of genipin in cells is not only confined to UCP2 Jürgen Kreiter University of Veterinary Medicine, Vienna, Austria
P2 a/9 Functional and structural characterization of isolated mammalian mitochondrial respiratory supercomplex I+III2 James A. Letts Institute of Science and Technology Austria, Klosterneuberg, Austria P2 a/10 The effect of lipid phase transitions in mitochondrial membranes on respiration and OxPhos system supercomplex formation Semen V. Nesterov Institute of Cytochemistry and Molecular Pharmacology, Moscow, Russian Federation
SCIENTIFIC PROGRAM
P2 a/11 PFOA modulates activity and gene and protein expressions of UCP1 in brown adipocytes Patrícia Reckziegel Department of Molecular Bioscience, The Wenner-Gren Institute, Stockholm University, Sweden; Department of Pharmacology, Biomedical Sciences Institute, University of São Paulo, Brazil P2 a/12 Molecular bases of mitochondrial uncoupling protein 1 induced thermogenesis Mathilde S. Piel Université Paris-Diderot, Paris, France; Institut de Biologie Physico Chimique, Paris, France P2 a/13 The role of Rcf1 as a modulator of the respiratory chain in yeast Jacob Schäfer Department of Biochemistry and Biophysics, Arrhenius Laboratories, Stockholm University, Sweden P2 a/14 Purification and analysis of a respiratory supercomplex in the Firmicutes Bacillus subtilis and Geobacillus stearothermophilus Nathalie Sisattana Institut de Biologie Physico-Chimique, UMR 7099 CNRS/Université Paris Diderot, France P2 a/15 Protein turnover within mitochondrial complexes Ilka Wittig Functional Proteomics, Goethe University, Frankfurt, Germany P2 a/16 Mitochondrial respiratory states and rates: building blocks of mitochondrial physiology Erich Gnaiger D. Swarovski Research Laboratory, Department of Visceral, Transplant and Thoracic Surgery, Medical University of Innsbruck, Oroboros Instruments, Innsbruck, Austria P2 a/17 Absence of UCP3 impacts mitochondrial glycerol-3-phosphate dehydrogenase activity and enzymatic antioxidant capacity in brown adipose tissue of mice acclimated at thermoneutrality Assunta Lombardi Department of Science and Technology University of Sannio, Italy
SCIENTIFIC PROGRAM
P2 b/1 Nitrite lowers the oxygen cost of ATP supply in skeletal muscle cells by stimulating the rate of glycolytic ATP synthesis Charles Affourtit School of Biomedical Sciences, University of Plymouth, United Kingdom P2 b/2 Molecular mechanisms of ischemic kidney injury and protection: the role of mitochondria Nadezda V. Andrianova Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, Moscow, Russian Federation P2 b/3 Short term cultivation of human amniotic mesenchymal stromal cells at atmospheric oxygen causes metabolic switch to oxidative phosphorylation Asmita Banerjee Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, AUVA Research Center, Vienna, Austria P2 b/4 Modulating cellular flavin cofactor levels in humans via the bi-functional and monofunctional FLAD1 gene products Maria Barile Department of Biosciences, Biotechnology, and Biopharmaceutics, University of Bari Aldo Moro, Bari, Italy P2 b/5 Ubiquitin-dependent degradation of mitochondrial proteins regulates energy metabolism Giovanni Bénard Laboratoire Maladies Rares: Génétique et Métabolisme- INSERM U1211, Bordeaux, France; Université de Bordeaux, France P2 b/6 ATP, the forgotten nucleotide in relationship between metabolism and body mass of individuals Mélanie Boël Laboratoire d'Ecologie des Hydrosystèmes Naturels et Anthropisés, Université de Lyon, ENTPE, France P2 b/7 Aerobic metabolism of endothelial cells chronically exposed to statins Izabela Broniarek Department of Bioenergetics, Faculty of Biology, Adam Mickiewicz University in Poznan, Poland P2 b/8 Role of mitochondrial Ca2+ in the interplay between circadian core clock genes and cellular energetic metabolism Olga Cela Department of Clinical and Experimental Medicine, University of Foggia, Italy
SCIENTIFIC PROGRAM
P2 b/9 Real-time imaging of mitochondrial ATP dynamics discloses the metabolic setting of single cells Maria R. Depaoli Gottfried Schatz Research Center, Molecular Biology and Biochemistry, Medical University of Graz, Austria P2 b/10 Analysis of mitochondrial ATP synthase cluster formation in insulin-secreting INS-1E cells as visualized by 3D super-resolution dSTORM microscopy Andrea Dlasková Institute of Physiology, Department of Mitochondrial Physiology, Prague, Czech Republic P2 b/11 Respiratory mapping of mitochondrial pathways for establishing a database of mitochondrial physiology Carolina Doerrier Oroboros Instruments, Innsbruck, Austria P2 b/12 Activation of microglial cells requires metabolic shifts towards glycolysis Amalia M. Dolga Department of Molecular Pharmacology, Groningen Research Institute of Pharmacy, University of Groningen, The Netherlands P2 b/13 Bioenergetic basis for the effects of arginine and alpha-ketoglutarate on lifespan Dmytro Gospodaryov Department of Biochemistry and Biotechnology, Vasyl Stefanyk Precarpathian National University, Ivano-Frankivsk, Ukraine P2 b/14 How Mitochondrial Src Kinase Modulates Mitochondrial Functions? Hala Guedouari Department of Biology, Université de Moncton, NB, Canada P2 b/15 Mitochondria impacts DNA methylation and gene expression through modulation of methionine and polyamine metabolism Janin H. Santos National Institute of Environmental Health Sciences, NIH, USA P2 b/16 Hibernating mitochondria, the way to confer cell stress? Koen D.W. Hendriks Department of Clinical Pharmacy and Pharmacology, University Medical Centre Groningen, University of Groningen, The Netherlands
SCIENTIFIC PROGRAM
P2 b/17 Branched chain 2-oxoacid metabolism supplies mitochondrial H2O2 and ATP to stimulate insulin secretion Blanka Holendová, Lydie Plecitá-Hlavatá Department of Mitochondrial Physiology, Institute of Physiology, AS CR, Prague, Czech Republic
P2 b/18 Electron supply to the Q-junction: assessment of mitochondrial respiration, H2O2 flux and the redox state of the Q-pool Timea Komlodi Oroboros Instruments, Innsbruck, Austria P2 b/19 Endogenous quinones sustain NADH oxidation by complex I during anoxia, supporting substrate-level phosphorylation in mouse liver mitochondria Timea Komlódi Department of Medical Biochemistry, Semmelweis University, Budapest, Hungary P2 b/20 mTORC1 inhibition affects mitochondrial morphology Hana Nuskova Division of Signal Transduction in Cancer and Metabolism, German Cancer Research Center (DKFZ), Heidelberg, Germany P2 b/21 Development of a new chemical inhibitor of OPA1 Activity to increase cancer cell sensitivity toward apoptosis Anna Pellattiero Department of Biology, University of Padua, Venetian Institute of Molecular Medicine, Padua, Italy
P2 b/22 Dietary supplementation with MicroActive Coenzyme Q10 increases plasma and skeletal muscle Coenzyme Q10 levels in Thoroughbred horses that are genetically variable for basal Coenzyme Q10 activity levels Mary F. Rooney School of Biochemistry and Immunology, Trinity Biomedical Sciences Institute (TBSI), Trinity College Dublin, Ireland P2 b/23 Searching of metabolic markers of successful anhydrobiosis in tardigrades Milena Roszkowska Department of Bioenergetics, Adam Mickiewicz University, Poznań, Poland P2 b/24 Dysfunction of the immune system in conditional IF1 (Atp5if1-/-) knockout mice in colon Fulvio Santacatterina Departamento de Biología Molecular, Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas- Universidad Autónoma de Madrid (CSIC-UAM); Centro de Investigación Biomédica en Red de Enfermedades Raras (CIBERER), Universidad Autónoma de Madrid, Spain SCIENTIFIC PROGRAM
P2 b/25 Age dependent loss of mitochondrial function in epithelial tissue can reversed by Coenzyme Q10 Daniel Schniertshauer Department of Life Sciences, Albstadt-Sigmaringen University of Applied Sciences, Sigmaringen, Germany P2 b/26 Metabolic profiling and myoglobin expression in human neonatal and adult mesenchymal stem cells Rosella Scrima Department of Clinical and Experimental Medicine, University of Foggia, Italy P2 b/27 Mitochondrial activity differs in two sub-regions of the human amniotic membrane Adelheid Weidinger Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, AUVA Research Center, Austrian Cluster for Tissue Regeneration, Vienna, Austria
P2 b/28 KMADP for oxidative phosphorylation depends on substrate oxidative capacity Wayne T. Willis Department of Medicine, University of Arizona, Tucson, USA P2 b/29 Development of a kit to measure absolute millivolts of mitochondrial and plasma membrane potentials in cell cultures Akos A. Gerencser Buck Institute for Research on Aging, Novato, Image Analyst Software, Novato, CA, USA P2 b/30 Control analysis of cellular energy metabolism in intact, adherent cell cultures Akos A. Gerencser Buck Institute for Research on Aging, Novato, Image Analyst Software, Novato, CA, USA P2 b/31 Unravelling the roles of Fission Protein 1: a forgotten mitochondrial fission factor with pleiotropic functions Tiago Branco Fonseca Venetian Institute of Molecular Medicine, Padova, Italy
P3 a/1 Self-assembled proteolipossomes to functionally characterize the alternative oxidase from Moniliophthora perniciosa Mario R. O. Barsottini Institute of Biology, State University of Campinas, Federative Republic of Brazil SCIENTIFIC PROGRAM
P3 a/2 Role of the 2Fe-2S Rieske cluster in protection against ROS generation by cytochrome bc1 complex Łukasz Bujnowicz Department of Molecular Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland P3 a/3 Functional and structural characterization of Alternative Complex III Filipa Calisto Instituto de Tecnologia Química e Biológica – António Xavier, Universidade Nova de Lisboa, Oeiras, Portugal
P3 a/4 “Independent motion” model for the Qo site of cytochrome bc1 implicated from the combinatorial mutational studies in Rhodobacter capsulatus Robert Ekiert Department of Molecular Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland P3 a/5 Prediction of AOX inhibitors from molecular structure Alicia Rosell Hidalgo Department of Biochemistry and Biomedicine, School of Life Sciences, University of Sussex, Brighton, United Kingdom P3 a/6 Molecular effects of human cytochrome b mutation G34S causing exercise intolerance studied in bacterial model Patryk Kuleta Department of Molecular Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland
P3 a/7 Structural and functional characterization of the interaction of yeast cytochrome bc1 complex with recombinant cytochrome c Vishnupriya Pandey Institute for Biochemistry and Molecular Biology, ZBMZ, Faculty of Medicine, University of Freiburg, Germany
P3 a/8 Thermodynamic properties of two magnetically distinct forms of semiquinone in the catalytic Qi site of cytochrome bc1 Sebastian Pintscher Department of Molecular Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland P3 a/9 Role of the Q-loop for the function of Escherichia coli cytochrome bd oxidase Alexander Theßeling Institut für Biochemie, Albert-Ludwigs-Universität, Freiburg, Germany
SCIENTIFIC PROGRAM
P3 a/10 Cryo-EM structure of the alternative complex III from Rhodothermus marinus Janet Vonck Department of Structural Biology, Max Planck Institute of Biophysics, Frankfurt am Main, Germany P3 a/11 Comparison of the kinetic parameters of alternative oxidases purified from Trypanosoma brucei, Sauromatum guttatum and Arabidopsis thaliana Fei Xu Biochemistry and Biomedicine, School of Life Sciences, University of Sussex, Falmer, United Kingdom; Applied Biotechnology Center, Wuhan Institute of Bioengineering, Wuhan, China P3 a/12 Development of novel AOX inhibitors for fungicidal resistance management Luke Young Biochemistry & Biomedicine, School of Life sciences, University of Sussex, Falmer, Brighton, United Kingdom
P4 a/1 Exploring the reductive phase of cytochrome c oxidase: assignment of heme’s redox states and relative structure changes through potential-resolved FTIR Federico Baserga Department of Physics, Freie Universität Berlin, Germany P4 a/2 Control of transmembrane charge transfer in cytochrome c oxidase by the membrane potential Markus L. Björck Department of Biochemistry and Biophysics, The Arrhenius Laboratories for Natural Sciences, Stockholm University, Sweden P4 a/3 Conformational transitions of respiratory cytochrome c oxidase Marian Fabian Center for Interdisciplinary Biosciences, Technology and Innovation Park, P. J. Šafárik University, Košice, Slovakia P4 a/4 EPR detection of radical(s) in cytochrome c oxidase Daniel Jancura Department of Biophysics, Faculty of Science, Safarik University, Kosice, Slovakia P4 a/5 A novel setup for time-resolved IR spectroscopy on cytochrome c oxidase Pit Langner Experimental Molecular Biophysics, Department of Physics, Freie Universität Berlin, Germany SCIENTIFIC PROGRAM
P4 a/6 Definition of the electron transfer pathway between cytochrome c and cytochrome oxidase Francis Millett Department of Chemistry and Biochemistry, University of Arkansas, Fayetteville, AR, USA P4 a/7 Role of subunit COX8A in biogenesis of cytochrome c oxidase in human fibroblasts Daria Rotko Nencki Institute of Experimental Biology, Warsaw, Poland
P4 a/8 Towards time-resolved structural studies of ba3-type cytochrome c oxidase Cecilia Safari University of Gothenburg, Department of Chemistry and Molecular Biology, Sweden P4 a/9 Novel insights in to lipid-protein interactions from large scale atomistic molecular dynamics simulations of cytochrome c oxidase Vivek Sharma Department of Physics, University of Helsinki, Institute of Biotechnology, Finland P4 a/10 Extraction and reconstitution of S. cerevisiae cytochrome c oxidase in liposomes without the use of detergent Irina A. Smirnova Department of Biochemistry and Biophysics, the Arrhenius Laboratories for Natural Sciences, Stockholm University, Sweden P4 a/11 Computational investigation of the hydration of the H channel of bovine heart cytochrome c oxidase Bo Thomsen, Y. Sugita Theoretical Molecular Science Laboratory, RIKEN, 2-1 Hirosawa, Wako, Japan P4 a/12 The stochastic modelling of proton pumping: the contribution of the K-channel Victoria Titova Institute of Cytochemistry and Molecular Pharmacology, Moscow, Russian Federation P4 a/13 Protonation state and conformational changes at the K-channel entry of cytochrome c oxidase Alexander Wolf Department of Physics, Freie Universität Berlin, Germany P4 a/14 Physiological and structural analysis of cytochrome c oxidase activating protein Higd1a Yuya Nishida Medical Biochemistry, Osaka University Graduate School of Medicine, Osaka, Japan
SCIENTIFIC PROGRAM
P4 a/15 Proton kinetics in ba3 oxidase Federica Poiana Stockholm University, Sweden
P4 b/1 Creatine Transporter (CrT) deficiency impairs brain energetics under stress Hong-Ru Chen Department of Neuroscience, University of Virginia School of Medicine, Charlottesville; Department of Pediatrics, Emory University School of Medicine, Atlanta, USA P4 b/2 Hexokinase is the main modulator of H2O2 production and calcium handling during post-natal brain development in mitochondria Eduardo S. Ferreira Department of Medical Biochemistry Leopoldo de Meis, IBqMLM-Federal University of Rio de Janeiro, Brazil P4 b/3 Perinatal asphyxia and brain development: mitochondrial damage without anatomical or cellular losses Antonio Galina Institute of Medical Biochemistry Leopoldo de Meis, Rio de Janeiro, Brasil P4 b/4 Novel pathogenic heteroplasmic substitution m.14597 A>G in the MT-ND6 gene in female with Leber’s hereditary optic neuropathy Tatiana D. Krylova Research Centre For Medical Genetics, Moscow, Russian Federation P4 b/5 Characterization of reversible loss of flavin from mitochondrial complex I in ischemic brain injury Anna Stepanova Department of Pediatrics, Columbia University, New York, USA P4 b/6 Systems biology identifies preserved integrity but impaired metabolism of mitochondria due to a glycolytic defect in Alzheimer’s disease neurons Niamh M. C. Connolly Department of Physiology & Medical Physics, Royal College of Surgeons in Ireland, Dublin, Ireland
SCIENTIFIC PROGRAM
P5 a/1 ATP synthase purification and subunit composition analysis in the models of mammalian Complex V deficiencies Iuliia Efimova Department of Bioenergetics, Institute of Physiology, The Czech Academy of Sciences, Prague, Czech Republic P5 a/2 Bioinformatic analysis of prokaryotic F-type ATP synthase operons Boris A. Feniouk Faculty of Bioengineering and Bioinformatics, A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Russian Federation P5 a/3 Single particle analysis of V1-ATPase from thermophilic bacterium Aya Furuta Department of Molecular Biosciences Kyoto Sangyo University, Kyoto, Japan P5 a/4 Structure, mechanism, and regulation of the chloroplast ATP synthase Alexander Hahn Department of Structural Biology, Max-Planck-Institute of Biophysics, Frankfurt, Germany
P5 a/5 Water molecules in proton half-channels of F0F1-ATP synthase: prediction and implications Leonid A. Ivontsin Institute of Cytochemistry and Molecular Pharmacology, Moscow, Russian Federation P5 a/6 Role of ATPase Inhibitory Factor 1 (IF1) in metabolic regulations of insulin secreting INS-1E cells Anežka Kahancová Institute of Physiology, Czech Academy of Sciences, Prague, Czech Republic
P5 a/7 Regulatory C-terminal domain of the ε subunit in FoF1 ATP synthase is important to maintain cellular membrane potential by activating ATP-dependent H+ pumping in Bacillus subtilis Yasuyuki Kato-Yamada Department of Life Science, College of Science, Rikkyo University, Tokyo, Japan P5 a/8 De novo designed axis works as a rotor of rotary motor Jun-ichi Kishikawa Department of Molecular Biosciences, Kyoto Sangyo University, Kyoto, Japan P5 a/9 Proton translocation, force generation and dimerization in the F-type ATP synthase of Polytomella sp. Niklas Klusch Department of Structural Biology; Frankfurt, Germany SCIENTIFIC PROGRAM
P5 a/10 Single-molecule analysis of bovine mitochondrial F1-ATPase for direct assignment of crystal structures and rotational pausing states Ryohei Kobayashi Department of Applied Chemistry, School of Engineering, University of Tokyo, Japan P5 a/11 A disease-causing hydrogen bond in ATP synthase of mitochondrial genetic origin Roza Kucharczyk Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland P5 a/12 Phonon assisted proton ATPase - electron tunnelling model Elena Lacatus Polytechnic University of Bucharest, Romania
P5 a/13 Biochemical properties of Escherichia coli FOF1-ATP synthase with Leu249Gln substitution in beta subunit Anna S. Lapashina Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, Russian Federation 2+ 2+ P5 a/14 ADP/ Mg inhibition of F1 ATPases is tuned using Mg affinity governed by species-specific γ subunit contacts to α and β subunits Duncan G. G. McMillan Department of Biotechnology, Delft University of Technology, The Netherlands P5 a/15 Cryo EM structure of intact rotary H+-ATPase/synthase from Thermus thermophilus Atsuko Nakanishi Department of Molecular Biosciences, Kyoto Sangyo University, Japan P5 a/16 Subunit a of ATP synthase – essential for ATP synthase dimerization in Saccharomyces cerevisiae Katarzyna Niedzwiecka, Emilia Baranowska Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland P5 a/17 The influence of vacuolar Proton-Translocating ATPase (V-ATPase) in health and disease through the lens of glycolysis Karlett J. Parra Department of Biochemistry and Molecular Biology, University of New Mexico Health Sciences Center, Albuquerque, NM, USA
SCIENTIFIC PROGRAM
+ P5 a/18 The effect of point mutations in Escherichia coli H -FOF1 ATP synthase on the MgADP-inhibition of ATPase activity Tatiana E. Shugaeva Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, Russian Federation P5 a/19 Membrane potential driven ATP synthesis in single reconstituted F-ATP synthase Hendrik Sielaff Single Molecule Microscopy Group, Jena University Hospital, Jena, Germany
P5 a/20 Chemo-mechanical coupling mechanism of rotation of mammalian F1-ATPase by static and dynamic X-ray crystallographic studies Toshiharu Suzuki Department of Applied Chemistry, School of Engineering, The University of Tokyo, Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology, Department of Molecular Bioscience, Kyoto-Sangyo University, Kyoto, Japan
P5 a/21 Rotation of the engineered F1-ATPase with non-catalytic α-type P-loops Hiroshi Ueno Department of Applied Chemistry, Graduate School of Engineering, The University of Tokyo, Japan
P5 a/22 Electrophysiological properties of channel formed by bovine FOF1 ATP synthase in planar lipid bilayer Andrea Urbani Department of Biomedical Sciences, University of Padova, Padova, Italy P5 a/23 Vacuolar-ATPase E subunit somatic missense mutations identified in the COSMIC database result in increased enzyme activity Bradleigh Whitton Faculty of Medicine, Cancer Sciences Unit, University of Southampton, Southampton General Hospital Tremona Road Southampton, United Kingdom P5 a/24 Mechanism of light regulation on chloroplast ATP synthase revealed by single-particle cryo-EM Jay-How Yang Center for Applied Structural Discovery (CASD), Biodesign Institute, Arizona State University, Tempe, AZ, USA
SCIENTIFIC PROGRAM
P5 a/25 The chemomechanical coupling of F1 ATPase of Paracoccus denitrificans Mariel Zarco-Zavala Department of Applied Chemistry, Graduate School of Engineering, The University of Tokyo, Japan
P5 a/26 The high-resolution structure and inhibition of Trypanosoma brucei F1-ATPase Alena Zíková Biology Centre ASCR, Institute of Parasitology, Ceske Budejovice, Czech Republic P5 a/27 Mechanistic features of subunit ε of the Mycobacterial F-ATP synthase Nebojša Bogdanović School of Biological Sciences, Nanyang Technological University, Singapore
P5 b/1 Drug development for human African trypanosomiasis targeting its cyanide-insensitive respiration Yasutoshi Kido Department Parasitology, Grad. Sch. Med., Osaka City University, Japan
P6 a/1 Proton translocations in channelrhodopsin-1 from Chlamydomonas augustae Ramona Schlesinger Freie Universität Berlin, Department of Physics, Genetic Biophysics, Berlin, Germany
P6 b/1 Disease-segregating Aifm1 mutation causes myopathy in knockin mice Daniele Bano Deutsches Zentrum für Neurodegenerative Erkrankungen (DZNE, Bonn), Germany P6 b/2 Repeated exposure to hyperbaric hyperoxia affects mitochondrial functions of the lung fibroblasts Jiri Dejmek Institute of Biophysics, Biomedical Center, Czech Republic
SCIENTIFIC PROGRAM
P6 b/3 Assessing the role of mitochondrial glycerol 3-phosphate dehydrogenase in the antidiabetic effect of metformin - a mass spectrometry imaging study Ove Eriksson Meilahti Clinical Proteomics Core facility, HiLIFE, Faculty of Medicine, Biomedicum Helsinki, University of Helsinki, Finland P6 b/4 Cardioprotective effect of dehydrosilybin preconditioning in isolated rat heart Eva Gabrielova Department of Medical Chemistry and Biochemistr, Olomouc, Czech Republicy P6 b/5 Altered mitochondrial dynamics due to DNM2 mutations Anikó Gal MitoCare Center, Department of Pathology, Anatomy and Cell Biology, Thomas Jefferson University, Philadelphia, PA, USA; Institute of Genomic Medicine and Rare Disorders, Semmelweis University, Budapest, Hungary P6 b/6 Mitochondrial bioenergetics in skeletal muscle of premanifest and early manifest transgenic minipig model for Huntington’s disease Hana Hansikova Laboratory for Study of Mitochondrial Disorders, Department of Pediatrics and Adolescent Medicine, First Faculty of Medicine, Charles University and General University Hospital in Prague, Czech Republic P6 b/7 Creating patient-specific stem cell models and characterizing metabolic changes in Leigh’s Syndrome Shilpa Iyer Department of Biological Sciences, Fulbright College of Arts and Sciences, Univ of Arkansas, Fayetteville, USA P6 b/8 Aerobic metabolism adaptations of human endothelial EA.hy926 cells related to chronic hypoxia Agnieszka Koziel Department of Bioenergetics, Adam Mickiewicz University, Poznan, Poland P6 b/9 Impact of sepsis on mitochondrial respiration of the porcine kidney Jitka Kuncova Institute of Physiology, Faculty of Medicine in Plzeň, Charles University, Pilsen, Czech Republic P6 b/10 Mitochondrial ATP production is required for Wnt signaling modulation Luigi Leanza Department of Biology, University of Padova, Italy
SCIENTIFIC PROGRAM
P6 b/11 Investigating the role of the mitochondria shaping protein Opa1 in controlling adipocyte size Marta Medaglia University of Padova, Department of Biology, Padova, Italy P6 b/12 The role of mitochondrial superoxide release in acute and chronic hypoxia sensing and signaling of the pulmonary vasculature Oleg Pak Excellence Cluster Cardiopulmonary System, University of Giessen and Marburg Lung Center (UGMLC), member of the German Center for Lung Research (DZL), Justus-Liebig-University, Giessen, Germany P6 b/13 Bioenergetics of the Costello syndrome Rodrigue Rossignol INSERM U1211, 33000 Bordeaux, France P6 b/14 Mitochondrial DNA copy number and Complex I activity in pediatric acute lymphoblastic leukemia Archna Singh Department of Biochemistry, All India Institute of Medical Sciences, New Delhi, India P6 b/15 Evaluating the bioenergetics of myoblast migration on aligned nanofiber scaffolds Kalyn S. Specht Virginia Tech, Department of Human Nutrition, Foods, and Exercise and the Virginia Tech Center for Drug Discovery, Blacksburg, USA P6 b/16 X-ray crystallographic structure analysis of seven disease-causing mutants of human lipoamide dehydrogenase Eszter Szabo Department of Medical Biochemistry, MTA-SE Laboratory for Neurobiochemistry, Semmelweis University, Budapest, Hungary P6 b/17 Alterations in energy transfer pathways in Wfs1 deficient mice Kersti Tepp Laboratory of Bioenergetics, National Institute of Chemical Physics and Biophysics, Tallinn, Estonia P6 b/18 ATP synthase assembly defect and liver damage are major pathology hallmarks of inducible Tmem70 knockout in adult mice Marek Vrbacky Institute of Physiology, Czech Academy of Sciences, Prague, Czech Republic
SCIENTIFIC PROGRAM
P7 a/1 Epoxycyclohexenedione-type Compounds Make Up a New Class of Inhibitors of the Bovine Mitochondrial ADP/ATP Carrier Ayaki Aoyama Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto, Japan P7 a/2 Biophysical and pharmacological characterization of a channel-forming mitochondrial protein complex Vanessa Checchetto Department of Biology, University of Padova, Italy P7 a/3 The putative role of human VDAC1 in Huntington disease pathogenesis Daria Grobys Department of Bioenergetics, Faculty of Biology, Adam Mickiewicz University, Poznań, Poland P7 a/4 The LAT1 amino acid transporter: substrate binding site and drug discovery Cesare Indiveri Department DiBEST (Biologia, Ecologia, Scienze della Terra) Unit of Biochemistry and Molecular Biotechnology, University of Calabria, Arcavacata di Rende, Italy P7 a/5 Control of brown adipose thermogenesis by a cold-inducible, circadian mitochondrial transporter Iuliia Karavaeva Section for Metabolic Genetics at the Novo Nordisk Foundation Center for Basic Metabolic Research, University of Copenhagen, Denmark P7 a/6 The influence of flavonoids on oxygen consumption and membrane potential of isolated endothelial mitochondria via modulation of mitoBKCa channel activity Anna Kicinska Department of Bioenergetics, Adam Mickiewicz University, Poznan, Poland P7 a/7 Recording channel activity of the ROMK1/2 protein produced in E. coli Piotr Koprowski Laboratory of Intracellular Ion Channels, Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warsaw, Poland P7 a/8 SK channel activation induces neuroprotection by metabolic reprogramming Inge E. Krabbendam Dept. of Molecular Pharmacology, University of Groningen, The Netherlands
SCIENTIFIC PROGRAM
P7 a/9 Production of ROMK1/2 protein in E. coli for functional studies Milena Krajewska Laboratory of Intracellular Ion Channels, Department of Biochemistry, Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warsaw, Poland
P7 a/10 BK-DEC splice variant forms a functional BKCa channel in the inner mitochondrial membrane Shur K. Kucman Laboratory of Intracellular Ion Channels, Nencki Institute of Experimental Biology Polish Academy of Science, Warsaw, Poland P7 a/11 Calcineurin-homologous proteins bind with high affinity to the CBD-region of the human Na+/H+ exchanger NHE1 Shuo Liang Institute for Biochemistry and Molecular Biology, ZBMZ, Faculty of Medicine, Faculty of Biology, University of Freiburg, Germany P7 a/12 A specific mechanism based on alternative 5’UTRs controls the VDAC1 translation in D. melanogaster Angela Messina Department of Biological, Geological and Environmental Sciences, University of Catania, Italy P7 a/13 Role of the essential protein LETM1 in mitochondrial cation regulation Karin Nowikosky Department of Internal Medicine I and Comprehensive Cancer Center, Medical University of Vienna, Austria P7 a/14 Novel psoralen-derivatives with increased solubility in cancer treatment Roberta Peruzzo Department of Biology, University of Padua, Italy P7 a/15 Following kinetic processes of membrane proteins in real time using fluorescence microscopy Thomas Schick University of Bern, Department of Chemistry and Biochemistry, Graduate School for Cellular and Biomedical Sciences, Bern, Switzerland P7 a/16 Loss of mitochondrial phosphate carrier SLC25A3 in skeletal muscle provides evidence for compensatory but lower capacity phosphate uptake into mitochondria Erin L. Seifert MitoCare Center for Mitochondrial Imaging Research and Diagnostics, Department of Pathology, Anatomy and Cell Biology, Thomas Jefferson University, USA
SCIENTIFIC PROGRAM
P7 a/17 Purification of a seven-subunit Mrp-type sodium-proton antiporter Julia Steiner Institute of Science and Technology, Austria P7 a/18 Delivery of functional genetic code into human mitochondrion Natalya Verechshagina Laboratory of Molecular Genetics Technologies, Immanuel Kant Baltic Federal University, Kaliningrad, Russian Federation P7 a/19 Dynamics of Mitochondrial Calcium Uniporter expression at different gestational age Polina Vishnyakova National Medical Research Center for Obstetrics, Gynecology and Perinatology, Moscow, Russian Federation
P7 a/20 Metal ion-substituted protoporphyrins as a tool to study regulation of the activity of mitoBKCa channel by hydrogen sulfide Agnieszka Walewska Laboratory of Intracellular Ion Channels, Department of Biochemistry, Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warsaw, Poland P7 a/21 The structure-function analysis of mitochondrial calcium uniporter (MCU) using a yeast expression system Takenori Yamamoto Institute for Genome Research, Faculty of Pharmaceutical Sciences, Tokushima University, Japan
P7 a/22 Role of dcu C4-dicarboxylate transporters in H2 production during fermentation of glucose or glycerol Satenik Mirzoyan Department of Biochemistry, Microbiology and Biotechnology, Yerevan State University, Armenia P7 a/23 Understanding Escherichia coli formate channels working direction during fermentation of the mixture of glucose, glycerol and formate at pH 7.5 Satenik Mirzovan Department of Biochemistry, Microbiology and Biotechnology, Faculty of Biology; Scientific-Research Institute of Biology, Yerevan State University, Armenia
P7 b/1 The effect of 2-ketobutyrate on mitochondrial substrate level phosphorylation David Bui Department of Medical Biochemistry, Semmelweis University, Budapest, Hungary SCIENTIFIC PROGRAM
P7 b/2 TRAP1 regulation in cancer metabolism: identification of new interactors Giuseppe Cannino Dipartimento di Scienze Biomediche, Università di Padova, Italia P7 b/3 Mitochondria ROS blocker OP2-113 downregulates the insulin receptor substrate-2 (IRS-2) and inhibits lung tumor growth Nivea Dias Amoedo INSERM U1211, Bordeaux University, Bordeaux, France P7 b/4 Mitochondrial Src kinase, a potential therapeutic target for breast cancer Marie-Ange Djeungoue-Petga Department of Biology, Université de Moncton, Canada P7 b/5 Decisive role of mitochondrial substrate level phosphorylation on the survival of glutaminolytic cancer cells Judit Dóczi Department of Medical Biochemistry, Semmelweis University, Budapest, Hungary P7 b/6 EIF3F regulates oxidative phosphorylation and metastasis in human lung adenocarcinoma Pauline Esteves Bordeaux University, Bordeaux, France P7 b/7 Application of stable isotope metabolomics to discover new drug targets for breast cancer treatment Aleksandr Klepinin National Institute of Chemical Physics and Biophysics, Tallinn, Estonia P7 b/8 Progression series of murine fibrosarcoma separates proliferative and invasive transformation characteristics Michaela Kripnerová Institute of Physiology, Faculty of Medicine in Pilsen, Charles University, Pilsen, Czech Republic P7 b/9 Bioenergetic changes in breast cancer cells by lithocholic acid Edit Mikó Departments of Medical Chemistry; MTA-DE Lendület Laboratory of Cellular Metabolism, Debrecen, Hungary P7 b/10 Exploring the roles of mitochondrial dynamics protein Opa1 in melanocytes and melanoma Akiko Omori Venetian Institute of Molecular Medicine, Padova, Italy SCIENTIFIC PROGRAM
P7 b/11 Mechanisms of coupling of glycolysis and oxidative phosphorylation in colon cancer. Do we progress in understanding of the Warburg effect? Zulfiya Orynbayeva Department of Surgery, Drexel University College of Medicine, Philadelphia, USA P7 b/12 The phosphotransfer network and metabolic plasticity of colon cancer cells Ljudmila Ounpuu National Institute of Chemical Physics and Biophysics, Tallinn, Estonia P7 b/13 Substrate oxidation differences in human glioma cells and their potential clinical significance Anna Sebestyén 1st Department of Pathology and Experimental Cancer Research, Semmelweis University, Budapest, Hungary P7 b/14 Mitochondrial 2HG production as a function of IDH2 and HOT in breast cancer cells Katarína Smolková Institute of Physiology, Department of Mitochondrial Physiology, Czech Academy of Sciences, Czech Republic P7 b/15 Different regulation of mitochondrial respiration in human colorectal and breast cancer clinical samples Laura Truu Laboratory of Bioenergetics, National Institute of Chemical Physics and Biophysics, Tallinn, Estonia
P7 b/16 The ATP synthase inhibitor protein IF1 plays a significant role in cancer metabolic flexibility Gianluca Sgarbi Department of Biomedical and Neuromotor Sciences, Lab of Biochemistry and Mitochondrial Pathophysiology, University of Bologna, Italy
P7 b/17 The ATP synthase inhibitor protein IF1 plays a significant role in cancer metabolic flexibility Alessandra Baracca Department of Biomedical and Neuromotor Sciences, Lab of Biochemistry and Mitochondrial Pathophysiology, University of Bologna, Italy P7 b/18 Mitochondrial function linked to metabolic differences in dysplastic and cancerous oral cells Marilena Karavyraki School of Biochemistry and Immunology, Trinity Biomedical Sciences Institute (TBSI), Trinity College Dublin, Ireland
SCIENTIFIC PROGRAM
P8 a/1 The study of proton transfer in photosystem II Krzysztof L. Buzar Freie Universität Berlin, Department of Physics, Theoretical Molecular Biophysics, Berlin, Germany P8 a/2 Evaluating the importance of cyclic electron flow around photosystem I in microalgae Suzanne Ferté IBPC, UMR7141, CNRS, Paris, France P8 a/3 To b or not to b: direct reduction of cytochrome b-563 by ferredoxin in higher plant photosynthetic cyclic electron flow in vitro? Nick Fisher MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing, Michigan, USA P8 a/4 DEM - the dynamic exchange membrane model. Polymorphism of lipid phases in plant thylakoid membranes Győző Garab Institute of Plant Biology, Biological Research Centre, Hungarian Academy of Sciences Szeged, Hungary; Faculty of Science, University of Ostrava, Czech Republic P8 a/5 Effect of high-light acclimation on photosynthetic apparatus of Norway spruce Václav Karlický Faculty of Science, University of Ostrava, Ostrava; Global Change Research Institute CAS, Brno, Czech Republic P8 a/6 Light-induced conformational changes in Photosystem II core complexes revealed by rapid-scan Fourier Transfrom Infrared spectroscopy Melinda Magyar Institute of Plant Biology, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary P8 a/7 Mechanistic insights into Calredoxin function in the presence and absence of calcium Giulia Maria Marchetti Institute of Plant Biology and Biotechnology, University of Muenster, Germany P8 a/8 Direct measurement of the excited states of carotenoids in LHCII trimers using fs-stimulated Raman Spectroscopy Francesco Saccon Queen Mary University of London. School of Biological and Chemical Sciences, London, United Kingdom
SCIENTIFIC PROGRAM
P8 a/9 Comparison of plastid ultrastructure under isoosmotic polyethylene glycol and salt stress Katalin Solymosi Department of Plant Anatomy, ELTE - Eötvös Loránd University, Budapest, Hungary P8 a/10 Ferredoxin: NADP oxidoreductase; connected to the first and the last steps of photosynthetic reactions Bettina Ughy Institute of Plant Biology, Biological Research Centre, Hungarian Academy of Sciences Szeged, Hungary P8 a/11 Impact of donor- and acceptor-side inhibition upon the light tolerance of photosystem II Sam Wilson School of Biological and Chemical Sciences, Queen Mary University of London, United Kingdom P8 a/12 Colocalization of autofluorescent and YFP tagged photosynthetic membrane proteins imaged by CLSM Gábor Steinbach Institute of Biophysics, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary
P9 /1 Large-scale analyses of PTP dynamics on individual mitochondria Camille Colin University BORDEAUX, ISM, NSysA Group, ENSCBP, Pessac, France P9 /2 Role of F-ATP synthase f subunit in dimer formation and PTP modulation Chiara Galber Department of Biomedical Sciences and CNR Neuroscience Institute, University of Padova, Italy P9 /3 The inhibitor protein IF1 modulates the permeability transition pore in a human tumorigenic cell model Valentina Giorgio Department of Biomedical Sciences and CNR Neuroscience Institute, University of Padova, Italy P9 /4 Regulation of the mitochondrial permeability transition pore by arginine residues of F-ATP synthase Lishu Guo Department of Biomedical Sciences, University of Padova, Italy P9 /5 Deletion of subunits of human ATP synthase and impact on the mitochondrial permeability transition Jiuya He Medical Research Council Mitochondrial Biology Unit, University of Cambridge, Cambridge Biomedical Campus, United Kingdom
SCIENTIFIC PROGRAM
P9 /6 Effect of anions on Cyclophilin D binding to F-ATP synthase: Implications for the permeability transition pore Giovanna Lippe Department of Agricultural, Food, Environmental and Animal Sciences, University of Udine, Italy P9 /7 The role of ATP synthase megachannel in mitochondrial permeability transition Nelli Mnatsakanyan Department of Internal Medicine, Yale University, New Haven, USA P9 /8 A mitochondrial therapy for Duchenne muscular dystrophy Anna Stocco Department of Biomedical Sciences and CNR Neuroscience Institute, University of Padova, Italy
P10 /1 Towards unidirectional reconstitution of membrane proteins into liposomes Andrea M. Amati Department for Chemistry & Biochemistry, University of Bern, Switzerland; Graduate School for Cellular and Biomedical Sciences, University of Bern, Switzerland P10 /2 The mitochondria-targeted antioxidant SkQ1 can carry adenosine 3′,5′-cyclic monophosphate, but not guanosine 3′,5′-cyclic monophosphate, through artificial and natural membranes Yuri N. Antonenko Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russian Federation P10 /3 Generation of a novel, personalised in vitro model to assess the impact of mitochondrial DNA variation upon bioenergetic function and susceptibility to hepatotoxicity Amy Ball MRC Centre for Drug Safety Science, University of Liverpool, United Kingdom P10 /4 The electron-bifurcating hydrogenase Hnd from Desulfovibrio fructosovorans Myriam Brugna Laboratoire de Bioénergétique et Ingénierie des Protéines, Aix-Marseille Université, CNRS, France P10/5 Conformational states of coenzymes FAD and NADH monitored by ultrafast spectroscopy Géza I. Groma Institute of Biophysics, Biological Research Centre, Hungarian Academy of Sciences Szeged, Hungary SCIENTIFIC PROGRAM
P10 /6 Microbial oxidative sulfur metabolism: the membrane-bound heterodisulfide reductase-like complex of the bacterium Aquifex aeolicus Marianne Guiral Institut de Microbiologie de la Méditerranée, Laboratoire de Bioénergétique et Ingénierie des Protéines UMR 7281, Marseille, France P10 /7 Exploring the energetics of ammonia oxidizing archaea Logan H. Hodgskiss Archaea Biology and Ecogenomics Division, University of Vienna, Austria P10 /8 Non-heme Fe cofactor insertion into respiratory nitric oxide reductase from Paracoccus denitrificans Maximilian Kahle Department of Biochemistry and Biophysics, Stockholm University, Sweden P10 /9 Graphene-functionalized interface: ballistic electron transport Elena Lacatus Polytechnic University of Bucharest, Romania P10 /10 Remodeled cardiolipin modify the biophysical properties of the mitochondrial inner membrane in response to the protonmotive force Luis Alberto Luévano-Martínez Department of Parasitology, Instituto de Ciências Biomédicas; Department of Biochemistry, Instituto de Química Universidade de São Paulo, Brazil + P10 /11 Role of F0F1-ATPase in H flux by Escherichia coli during lactose fermentation at different pHs Satenik Mirzoyan Scientific-Research Institute of Biology Faculty of Biology, Yerevan State University, Armenia P10 /12 Surface-enhanced Raman spectroscopy as a new tool to study peculiarities of mitochondria bioenergetics Evelina I. Nikelshparg Faculty of Biology, Moscow State University, Russian Federation P10 /13 How mitochondrial (ultra) structure affects mitochondrial function Elianne P. Bulthuis Department of Biochemistry, Radboud Institute for Molecular Life Sciences, Radboud Center for Mitochondrial Medicine, Radboudumc, Nijmegen, The Netherlands
SCIENTIFIC PROGRAM
P10 /14 Protons at the membrane water interface Peter Pohl Institute of Biophysics, Johannes Kepler University Linz, Austria P10 /15 The energy metabolism of Leishmania as a drug target Eduardo Rial Centro de Investigaciones Biológicas, CSIC, Madrid, Spain P10 /16 A novel hydroxylamine oxidoreductase from a thermoacidophilic volcanic methanotroph for survival of high ammonia stress Wouter Versantvoort Department of Microbiology, Institute for Water and Wetland Research, Radboud University Nijmegen, AJ, The Netherlands P10 /17 Measuring real-time bioenergetic behaviour of electrically stimulated muscle cells Anthony Wynne School of Biomedical Sciences, University of Plymouth, United Kingdom P10 /18 Mitochondrial uncouplers induce CpG methylation of the ICAM1 gene promoter in endothelial cells Roman A. Zinovkin Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russian Federation P10 /19 Mitochondria: An old organelle with new functions Zakaria A. Almsherqi Department of Physiology, National University of Singapore, Singapore P10 /20 Resolving the mechanism of proton pumping coupled to hydride transfer in E. coli Transhydrogenase Simone Graf University of Bern, Department of Chemistry and Biochemistry, Bern, Switzerland P10 /21 New mitochondrial function assay technology Barry R. Bochner Biolog, Inc., Hayward, CA, USA