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Revised 11/2010

NOVA COLLEGE-WIDE COURSE CONTENT SUMMARY BIO 205 - GENERAL (4 CR.)

Course Description

Examines morphology, , , , and control of . Emphasizes application of microbiological techniques to selected fields. Lecture 3 hours. Recitation and laboratory 3 hours. Total 6 hours per week.

General Course Purpose

This is a one semester college transfer-level course designed to meet the needs of science majors. The student is introduced to the morphology, genetics, physiology, ecology, and control of microorganisms, as well as to the of infectious and immunity. Standard microbiological techniques for isolating, culturing, handling, examining, and identifying microorganisms are acquired. A basic understanding of the of microorganisms might be useful to individual planning careers involving pure and/or applied research, diagnosis, prevention and treatment of pathogenic , handling and/or processing, agriculture, industrial processes utilizing microbes, water purification, etc.

Course Prerequisites/Co-requisites

Prerequisites are CHM 111, 112 and two of the following: BIO 101, 102, 110, 120, 141, 142 or division approval.

Course Objectives

The list of objectives given below is not intended to be comprehensive, but rather to serve as foundation upon which to build.

Lecture

Upon completion of this course, the student should be able to:

 list, classify, and contrast the main categories of microorganisms  draw and label the parts of a “typical” bacterial , giving possible functions for each part  contrast eukaryotic and prokaryotic cells  distinguish between and give examples of autotrophic and heterotrophic forms of ; between lithotrophic and organotrophic sources of carbon  draw a typical growth curve and describe its phases  discuss how oxygen levels, temperature, pH, and the osmotic environment may affect microbial growth  contrast bacterial transduction, bacterial transformation, and bacterial conjugation  describe the and nature of nucleic acids  relate the terms DNA, gene, chromosomes, and mutation  describe protein synthesis  outline the operon hypothesis of microbial gene control  list and discuss at least six properties of  describe the basic principles and techniques involved in “” and relate at least three areas in which DNA recombinant technology may bear valuable fruit  list the important events of and/or the significance of the following pathways associated with microbial • Glycolysis • Krebs cycle • Alcoholic fermentation • Lactic acid fermentation

1 • Electron transport system • Aerobic respiration • Anaerobic respiration  contrast photosynthesis in green with that of: • • Sulfur  describe the roles and associations of microorganisms in  list and discuss the principle reasons for and methods of controlling and/or eliminating microorganisms  provide the following for selected diseases caused by microorganisms: • Causative • Occurrence • Reservoir • Mode of transmission • Incubation period • Period of communicability • Method of control including specific treatment  outline and describe the human body’s defensive mechanisms against infectious diseases  list and describe at least five examples of the uses of microorganisms in applied microbiology

Laboratory

Upon completion of this course, the student should be able to:

 use aseptic technique in handling microorganisms  use standard techniques for quantitative measurement of microbial growth  apply standard microbiological techniques to the isolation, cultivation and identification of microorganisms

Major Topics to be Included

 Microbial • Archaebacteria • Cyanobacteria • Photo bacteria • Eubascteria o gram negative o gram positive • Fungi •  Techniques used in microbial taxonomy  Microbial techniques  Microbial cell structure, eukaryotic and prokaryotic  Microbial nutrition and cultivation  Microbial growth and cell division  : enzymes, cell energetics, cellular biosynthesis  , genetic engineering  Control and elimination of microbes  : , infectious diseases, immunity  (taxonomy, cycle, temperate vs. Lytic, medical significance)  Applied microbiology: food, dairy, industrial, pharmaceutical

2 Optional Topics

The instructor may wish to include coverage of eukaryotic and parasitic worms.

Field Trips: Field trips may be taken to one or more of the following places depending upon the time available and student interests.

 American Type Culture Collection  Anaerobe Lab, V.P.I. & State University  National Agricultural Library  National Institutes of Health  Local brewery  Local dairy  Local research and development firm  Local water purification

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