Pollen Patterns Form from Modulated Phases Asja Radja1, Eric M. Horsley1, Maxim O. Lavrentovich2*, and Alison M. Sweeney1*
[email protected] [email protected] *
[email protected] *
[email protected] 1Department of Physics and Astronomy, University of Pennsylvania, 209 S. 33rd St., Philadelphia, Pennsylvania 19104, U.S.A. 2Department of Physics and Astronomy, University of Knoxville, 1408 Circle Dr., Knoxville, Tennessee 37996, U.S.A *Co-corresponding authors 1 Abstract Pollen grains are known for their impressive variety of species-specific, microscale surface patterning. Despite having similar biological developmental steps, pollen grain surface features are remarkably geometrically varied. Previous work suggests that a physical process may drive this pattern formation and that the observed diversity of patterns can be explained by viewing pollen pattern development as a phase transition to a spatially modulated phase. Several studies have shown that the polysaccharide material of plant cell walls undergoes phase separation in the absence of cross-linking stabilizers of the mixed phase. Here we show experimental evidence that phase separation of the extracellular polysaccharide material (primexine) during pollen cell development leads to a spatially modulated phase. The spatial pattern of this phase-separated primexine is also mechanically coupled to the undulation of the pollen cell membrane. The resulting patterned pools of denser primexine form the negative template of the ultimate sites of sporopollenin deposition, leading to the final micropattern observed in the mature pollen. We then present a general physical model of pattern formation via modulated phases. Using analytical and numerical techniques, we find that most of the pollen micropatterns observed in biological evolution could result from a physical process of modulated phases.