DOCSLIB.ORG

ROLE of BIOACTIVE FOOD COMPOUNDS in ACUTE and CHRONIC INFLAMMATION Victor Pallarés López

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
ROLE of BIOACTIVE FOOD COMPOUNDS in ACUTE and CHRONIC INFLAMMATION Victor Pallarés López ROLE OF BIOACTIVE FOOD COMPOUNDS IN ACUTE AND CHRONIC INFLAMMATION Victor Pallarés López Dipòsit Legal: T.1472-2012 ADVERTIMENT. L'accés als continguts d'aquesta tesi doctoral i la seva utilització ha de respectar els drets de la persona autora. Pot ser utilitzada per a consulta o estudi personal, així com en activitats o materials d'investigació i docència en els termes establerts a l'art. 32 del Text Refós de la Llei de Propietat Intel·lectual (RDL 1/1996). Per altres utilitzacions es requereix l'autorització prèvia i expressa de la persona autora. En qualsevol cas, en la utilització dels seus continguts caldrà indicar de forma clara el nom i cognoms de la persona autora i el títol de la tesi doctoral. No s'autoritza la seva reproducció o altres formes d'explotació efectuades amb finalitats de lucre ni la seva comunicació pública des d'un lloc aliè al servei TDX. Tampoc s'autoritza la presentació del seu contingut en una finestra o marc aliè a TDX (framing). Aquesta reserva de drets afecta tant als continguts de la tesi com als seus resums i índexs. ADVERTENCIA. El acceso a los contenidos de esta tesis doctoral y su utilización debe respetar los derechos de la persona autora. Puede ser utilizada para consulta o estudio personal, así como en actividades o materiales de investigación y docencia en los términos establecidos en el art. 32 del Texto Refundido de la Ley de Propiedad Intelectual (RDL 1/1996). Para otros usos se requiere la autorización previa y expresa de la persona autora. En cualquier caso, en la utilización de sus contenidos se deberá indicar de forma clara el nombre y apellidos de la persona autora y el título de la tesis doctoral. No se autoriza su reproducción u otras formas de explotación efectuadas con fines lucrativos ni su comunicación pública desde un sitio ajeno al servicio TDR. Tampoco se autoriza la presentación de su contenido en una ventana o marco ajeno a TDR (framing). Esta reserva de derechos afecta tanto al contenido de la tesis como a sus resúmenes e índices. WARNING. Access to the contents of this doctoral thesis and its use must respect the rights of the author. It can be used for reference or private study, as well as research and learning activities or materials in the terms established by the 32nd article of the Spanish Consolidated Copyright Act (RDL 1/1996). Express and previous authorization of the author is required for any other uses. In any case, when using its content, full name of the author and title of the thesis must be clearly indicated. Reproduction or other forms of for profit use or public communication from outside TDX service is not allowed. Presentation of its content in a window or frame external to TDX (framing) is not authorized either. These rights affect both the content of the thesis and its abstracts and indexes. UNIVERSITAT ROVIRA I VIRGILI ROLE OF BIOACTIVE FOOD COMPOUNDS IN ACUTE AND CHRONIC INFLAMMATION Victor Pallarés López Dipòsit Legal: T.1472-2012 UNIVERSITAT ROVIRA I VIRGILI ROLE OF BIOACTIVE FOOD COMPOUNDS IN ACUTE AND CHRONIC INFLAMMATION Victor Pallarés López Dipòsit Legal: T.1472-2012 UNIVERSITAT ROVIRA I VIRGILI ROLE OF BIOACTIVE FOOD COMPOUNDS IN ACUTE AND CHRONIC INFLAMMATION Victor Pallarés López Dipòsit Legal: T.1472-2012 UNIVERSITAT ROVIRA I VIRGILI ROLE OF BIOACTIVE FOOD COMPOUNDS IN ACUTE AND CHRONIC INFLAMMATION Victor Pallarés López Dipòsit Legal: T.1472-2012 Victor Pallarés López Role Of Bioactive Food Compounds In Acute And Chronic Inflammation Ph. Doctoral Thesis Directed by Dr. Maria Teresa Blay Olivé Departament de Bioquímica i Biotecnologia Tarragona 2012 UNIVERSITAT ROVIRA I VIRGILI ROLE OF BIOACTIVE FOOD COMPOUNDS IN ACUTE AND CHRONIC INFLAMMATION Victor Pallarés López Dipòsit Legal: T.1472-2012 UNIVERSITAT ROVIRA I VIRGILI ROLE OF BIOACTIVE FOOD COMPOUNDS IN ACUTE AND CHRONIC INFLAMMATION Victor Pallarés López Dipòsit Legal: T.1472-2012 Departament de Bioquímica i Biotecnologia C/ Marcel·lí Domigo s/n Campus Sescelades 43007 Tarragona Tel. +34 977 55 84 97 Fax. +34 977 55 82 32 FAIG CONSTAR que aquest treball, titulat “Role of bioactive food compounds in acute and chonic inflammation”, que presenta Victor Pallarés López per a la obtenció de títol de Doctor, ha estat realitzat sota la meva direcció al Departament de Bioquímica i Biotecnologia d’aquesta universitat i que acompleix els requeriments per poder optar a Menció Europea. Tarragona, 5 de Novembre de 2012 La directora de la tesi doctoral Dra. M. Teresa Blay Olivé UNIVERSITAT ROVIRA I VIRGILI ROLE OF BIOACTIVE FOOD COMPOUNDS IN ACUTE AND CHRONIC INFLAMMATION Victor Pallarés López Dipòsit Legal: T.1472-2012 UNIVERSITAT ROVIRA I VIRGILI ROLE OF BIOACTIVE FOOD COMPOUNDS IN ACUTE AND CHRONIC INFLAMMATION Victor Pallarés López Dipòsit Legal: T.1472-2012 A la meva família UNIVERSITAT ROVIRA I VIRGILI ROLE OF BIOACTIVE FOOD COMPOUNDS IN ACUTE AND CHRONIC INFLAMMATION Victor Pallarés López Dipòsit Legal: T.1472-2012 UNIVERSITAT ROVIRA I VIRGILI ROLE OF BIOACTIVE FOOD COMPOUNDS IN ACUTE AND CHRONIC INFLAMMATION Victor Pallarés López Dipòsit Legal: T.1472-2012 "No sé com em deu veure el món, però al meu entendre, em sembla que he estat només com un nen que juga a la vora del mar, i que es diverteix buscant de tant en tant una pedra més polida i una petxina més bonica del normal, mentre que el gran oceà de la veritat s'exposava davant meu completament desconegut." Sir Isaac Newton UNIVERSITAT ROVIRA I VIRGILI ROLE OF BIOACTIVE FOOD COMPOUNDS IN ACUTE AND CHRONIC INFLAMMATION Victor Pallarés López Dipòsit Legal: T.1472-2012 UNIVERSITAT ROVIRA I VIRGILI ROLE OF BIOACTIVE FOOD COMPOUNDS IN ACUTE AND CHRONIC INFLAMMATION Victor Pallarés López Dipòsit Legal: T.1472-2012 Agraïments Bé, començo aquesta part després de finalitzar totes les altres sabent que no és menys important que cap altra, ja que sense la vostra aportació, consells, ajuda, companyia, bon rotllo, i més i més i més, no s’hagués pogut dur a terme aquesta tesi. Després d’aquesta petita “intró”, m’agradaria afegir que no em vull deixar a ningú, però tot pot passar, així que us demano disculpes a aquells que no estiguin entre aquests agraïments, però, si heu format part d’aquest període directa o indirectament, us en dono les gràcies també. Primer de tot, voldria agrair a la Mayte, la meva tutora, el temps que m’ha brindat, així com l’orientació i els consells que m’ha anat donant des de fa 5 anys, durant el treball de fi de carrera, el màster i ara el doctorat. Gràcies per donar-me l’oportunitat d’endinsar-me en el món de la investigació i de confiar en els meus passos, que finalment també formen part dels teus. També vull agrair-te tot l’esforç realitzat al final del trajecte. A pesar de totes les inconveniències que se’ns han presentat, hem pogut tirar-ho endavant. Gràcies per tot. Gràcies també a tots els profes, Montse, Anna, Juan, Cinta, Pepa, Santi, Gerard, Antoni, Teresa, Anna (Arola), Begoña i també al recent nouvingut Manuel (Molta sort!), que gràcies a petits consells, exposicions o xerrades informals he pogut aprendre a ser millor investigador, i en especial al Lluís, per confiar en mi en els primers passos, i al Miquel, qui sempre ha tingut una estona per resoldre qualsevol dubte. A tots els doctorands, companys de batalla, que hem conviscut durant aquests quatre anys començant pels que han passat: David, Helena, Sabina, Fisu, Isa, Gemma, Albert (Guardiola Coach), Esther, i Laura; en especial, un agraïment enorme a la Xim, que va ser la meva mentora al laboratori, ajudant-me en tot i més el que va poder, i amb la qual vaig compartir les meus primers passos al lab. I amb els que ara comparteixo els últims moments a la uni, com l’Anna (gaudeix de la segona), la corredora de fons Cris (sempre treballant), la Ligia, la Laura, l’Aleix (un eivissenc autèntic), l’Ester, la Maria, el Husam (La bar-ba-cooa), la Maria José, la Zara, la Neus (esa futbolista!), l’Adrià, la Noe i l’Anabel (madridista no convençuda, jejeje; i és que el destí vol fer-te canviar d’opinió!!). Junts hem passat aquests quatre anys entre “barbaquius”, dies de platja, nits de farra, sopars de Nadal i, de tant en tant, per què no dir-ho, moments al laboratori (Perquè sí, els doctorands també treballem, jeje). Gràcies per tots els moments! Al CTNS Team, l’Anna, la Shirle, el Josep, i sobretot al Toni: Gràcies per tots els consells i la vostra disposició!! I els bons moments que hem passat, dies de partit (Barça Time), sopars, etc. També van ser molt grans!! UNIVERSITAT ROVIRA I VIRGILI ROLE OF BIOACTIVE FOOD COMPOUNDS IN ACUTE AND CHRONIC INFLAMMATION Victor Pallarés López Dipòsit Legal: T.1472-2012 També vull agrair el suport de les tècniques que durant aquests anys m’han ajudat en tot el que he necessitat, la Vane, la Yaiza, i sobretot la Niurka (Niurkix) i la Rosa (mi Rosaaa), que sempre s’ofereixen per ajudar en qualsevol cosa. També, vull agrair al Santiago i la Pietat per la seva disposició durant la preparació dels laboratoris d’estudiants, sempre amb tot controlat. Al Secretary-Team, amb la Ingrid, la Montse, la Rosa, i l’Esther (encara que ja no hi és, sempre s’ha preocupat de tots els seus “nens”), que ens ajuden en tots els tràmits i en tots els temes burocràtics, sempre de bon grat, agrair la seva feina sempre ben feta. Moltes gràcies! Ei!! I també la Mari!! Quines xerrades pel passadís... Gràcies pels teus “Bon dia” i el teu bon rotllo! També, als membres d’Enologia, amb els que sempre es pot comptar quan es necessita, en especial, al Braulio i la Marta.
Load more

Recommended publications
  • The Structural Basis of Gas-Responsive Transcription by the Human Nuclear Hormone Receptor REV-Erbb
    PLoS BIOLOGY The Structural Basis of Gas-Responsive Transcription by the Human Nuclear Hormone Receptor REV-ERBb Keith I. Pardee1,2, Xiaohui Xu1,2,3, Jeff Reinking1,2,7¤a, Anja Schuetz4¤b, Aiping Dong4, Suya Liu5, Rongguang Zhang6, Jens Tiefenbach1,2, Gilles Lajoie5, Alexander N. Plotnikov4¤b, Alexey Botchkarev4, Henry M. Krause1,2*, Aled Edwards1,2,3,4* 1 Banting and Best Department of Medical Research, The Department of Molecular Genetics, University of Toronto, Toronto, Canada, 2 Terrence Donnelly Centre for Cellular & Biomolecular Research, University of Toronto, Toronto, Canada, 3 Midwest Center for Structural Genomics, University of Toronto, Toronto, Canada, 4 Structural Genomics Consortium, University of Toronto, Toronto, Canada, 5 Department of Biochemistry, University of Western Ontario, London, Ontario, Canada, 6 Midwest Center for Structural Genomics, Argonne National Lab, Argonne, Illinois, United States of America, 7 Department of Biology, State University of New York at New Paltz, New Paltz, New York, United States of America Heme is a ligand for the human nuclear receptors (NR) REV-ERBa and REV-ERBb, which are transcriptional repressors that play important roles in circadian rhythm, lipid and glucose metabolism, and diseases such as diabetes, atherosclerosis, inflammation, and cancer. Here we show that transcription repression mediated by heme-bound REV- ERBs is reversed by the addition of nitric oxide (NO), and that the heme and NO effects are mediated by the C-terminal ligand-binding domain (LBD). A 1.9 A˚ crystal structure of the REV-ERBb LBD, in complex with the oxidized Fe(III) form of heme, shows that heme binds in a prototypical NR ligand-binding pocket, where the heme iron is coordinately bound by histidine 568 and cysteine 384.
    [Show full text]
  • Study of the Role of the Adaptor Protein Myd88 in the Iron-Sensing Pathway
    Université de Montréal Study of the role of the adaptor protein MyD88 in the iron-sensing pathway and of the effect of curcumin in the development of anemia in a DSS-induced colitis mouse model By Macha Samba Mondonga Department of Microbiology and Immunology Faculty of Medicine Thesis presented to the Faculty of Graduate Studies in partial fulfillment of the Ph.D. degree in Microbiology, Immunology and Infectious Diseases 30 Aout 2018 © Macha Samba Mondonga, 2018 Université de Montréal Cette thése est intitulée: Study of the role of the adaptor protein MyD88 in the iron-sensing pathway and of the effect of curcumin in the development of anemia in a DSS-induced colitis mouse model Par Macha Samba Mondonga Department of Microbiology and Immunology Faculty of Medicine 30 Aout 2018 © Macha Samba Mondonga, 2018 RÉSUMÉ Étude du rôle de la protéine adaptatrice MyD88 dans la voie de détection du fer et de l’effet de la curcumine dans le développement de l'anémie dans un modèle murin de colite induite par le DSS L'hepcidine est l'hormone peptidique essentielle pour la régulation systémique de l'homéostasie du fer, en déclenchant la dégradation de la ferroportine et en évitant le développement d'une surcharge ou d'une carence en fer. L'expression de l'ARNm de l'hepcidine HAMP est contrôlée par deux voies principales, la voix de signalisation inflammatoire et la voie de détection en fer, BMP/SMAD4. Il a été rapporté que MyD88, la protéine adaptatrice impliquée dans l'activation des cytokines via la stimulation des récepteurs toll-like (TLRs), contribue à l'expression de l'hepcidine dans la voie inflammatoire.
    [Show full text]
  • Data-Driven Insights Into Ligands, Proteins, and Genetic Mutations
    Data-Driven Insights into Ligands, Proteins, and Genetic Mutations by Jing Lu A dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy (Bioinformatics) in the University of Michigan 2016 Doctoral Committee: Professor Heather A. Carlson, Chair Professor Charles L. Brooks III Assistant Professor Barry Grant Professor David S. Sept Professor Kerby A. Shedden © Jing Lu, 2016 Acknowledgements I would like to thank my advisor, Dr. Heather Carlson, for years of patient guidance, teaching, and support through the course of my PhD. I have learnt how to think critically and be rigorous in every step of research. I also want to express gratitude to my committee: Professor Charles L. Brooks III, Assistant Professor Barry Grant, Professor David S. Sept, Professor Kerby A. Shedden. Their advising is insightful and deepens my understanding of my research projects. I would like to thank Dr. Richard Smith for timely support for both my writing and research. For many Saturdays and Sundays, he promptly responds my requests for proofreading. Much of my work is built on his code in protein and ligand analysis. I would like to thank other members in Dr. Carlson’s lab for helping me with my work. Through the discussion with Dr. Jim Dunbar, I have learnt many critical ideas in Cheminformatics. Also, thank you to Sarah Graham and Jordan Clark for their tremendous friendship and willing to help with my writing. I would also thank previous members in Dr. Carlson’s lab. I would thank Dr. Phani Ghanakota for many late-night discussions and Dr.
    [Show full text]
  • Targeting Heme with Single Domain Antibodies
    Targeting Heme with Single Domain Antibodies Zélia Licínia Ferreira Gouveia Dissertation presented to obtain the Ph.D degree in Biochemistry Instituto de Tecnologia Química e Biológica António Xavier | Universidade Nova de Lisboa Oeiras, June, 2015 Targeting Heme with Single Domain Antibodies Zélia Licínia Ferreira Gouveia Dissertation presented to obtain the Ph.D degree in Biochemistry Instituto de Tecnologia Química e Biológica António Xavier | Universidade Nova de Lisboa Research work coordinated by: Oeiras, Junho, 2015 Targeting Heme with Single Domain Antibodies Zélia Licínia Ferreira Gouveia Supervisor: Dr. Miguel P.Soares Instituto Gulbenkian da Ciência Co-Supervisor: Dr. Smilja Todorovic Instituto de Tecnologia Química e Biológica PhD fellowship financed by Fundação para a Ciência e Tecnologia (Apoio financeiro da FCT e do FSE no âmbito do Quadro Comunitário de apoio, BD: SFRH/BD/44828/2008) Oeiras, Junho, 2015 Acknowledgements Firstly and primarily I would like to express my most sincere gratitude to my Ph.D supervisor Dr. Miguel P. Soares for accepting me as his Ph.D student and to trust me this Ph.D project. I want to thank him for his help, advices and for non-stop teaching and extremely helpful and constructive scientific discussions. Today, I am the scientist that you help to build, thank you very much! I extremely grateful to Dr. Miguel P. Soares for his understanding and support, in a particular period of my life in which I had to take several weeks of my work time to be and support my family as we were having a extremely difficult time in our life. For all of this and more, thank you very much! I am grateful to Dr.
    [Show full text]
  • (12) Patent Application Publication (10) Pub. No.: US 2011/0098193 A1 Kingsmore Et Al
    US 2011 0098193A1 (19) United States (12) Patent Application Publication (10) Pub. No.: US 2011/0098193 A1 Kingsmore et al. (43) Pub. Date: Apr. 28, 2011 (54) METHODS AND SYSTEMS FOR MEDICAL type (such as a disease symptom) of the trait, wherein the SEQUENCING ANALYSIS association of the relevant element with the relevant compo nent phenotype identifies the relevant element as an element (76) Inventors: Stephen F. Kingsmore, St. associated with the trait, wherein the relevant component Augustine, FL (US); Callum J. phenotype is a component phenotype having a threshold Bell, Santa Fe, NM (US) value of severity, age of onset, specificity to the trait or dis ease, or a combination, wherein the relevant element is an (21) Appl. No.: 12/910,764 element having a threshold value of importance of the ele ment to homeostasis relevant to the trait, intensity of the (22) Filed: Oct. 22, 2010 perturbation of the element, duration of the effect of the element, or a combination. The disclosed methods are based Related U.S. Application Data on a model of how elements affect complex diseases. The disclosed model is based on the existence of significant (60) Provisional application No. 61/254,115, filed on Oct. genetic and environmental heterogeneity in complex dis 22, 2009. eases. Thus, the specific combinations of genetic and envi ronmental elements that cause disease vary widely among the Publication Classification affected individuals in a cohort. The disclosed model is an (51) Int. Cl. effective, general experimental design and analysis approach C4OB 30/04 (2006.01) for the identification of causal variants in common, complex C4DB 60/2 (2006.01) diseases by medical sequencing.
    [Show full text]
  • Mechanisms of Paracrine and Endocrine Actions of Angiotensin II
    University of Tennessee, Knoxville TRACE: Tennessee Research and Creative Exchange Doctoral Dissertations Graduate School 8-2004 Mechanisms of paracrine and endocrine actions of Angiotensin II Suyeon Kim University of Tennessee, Knoxville Follow this and additional works at: https://trace.tennessee.edu/utk_graddiss Part of the Life Sciences Commons Recommended Citation Kim, Suyeon, "Mechanisms of paracrine and endocrine actions of Angiotensin II. " PhD diss., University of Tennessee, 2004. https://trace.tennessee.edu/utk_graddiss/4533 This Dissertation is brought to you for free and open access by the Graduate School at TRACE: Tennessee Research and Creative Exchange. It has been accepted for inclusion in Doctoral Dissertations by an authorized administrator of TRACE: Tennessee Research and Creative Exchange. For more information, please contact [email protected]. To the Graduate Council: I am submitting herewith a dissertation written by Suyeon Kim entitled "Mechanisms of paracrine and endocrine actions of Angiotensin II." I have examined the final electronic copy of this dissertation for form and content and recommend that it be accepted in partial fulfillment of the requirements for the degree of Doctor of Philosophy, with a major in Human Ecology. Naima Moustaid-Moussa, Major Professor We have read this dissertation and recommend its acceptance: Jay Whelan, Jung Han Kim, Brynn H. Jones, John Koontz Accepted for the Council: Carolyn R. Hodges Vice Provost and Dean of the Graduate School (Original signatures are on file with official studentecor r ds.) To the Graduate Council: I am submitting herewith a dissertationwritten by Suyeon Kim entitled "Mechanisms of paracrine and endocrineactions of Angiotensin II." I have examined the finalpaper copy of this dissertation for formand content and recommend that it be accepted in partial fulfillment of the requirements forthe degreeof Doctor of Philosophy, with a major in Human Ecology.
    [Show full text]
  • The Incidence and Functional Relevance of Intrinsic Disorder in Enzymes and the Protein Data Bank Shelly Deforte University of South Florida, [email protected]
    University of South Florida Scholar Commons Graduate Theses and Dissertations Graduate School 6-27-2016 Intrinsic Disorder Where You Least Expect It: The Incidence and Functional Relevance of Intrinsic Disorder in Enzymes and the Protein Data Bank Shelly Deforte University of South Florida, [email protected] Follow this and additional works at: http://scholarcommons.usf.edu/etd Part of the Bioinformatics Commons, Medicine and Health Sciences Commons, and the Molecular Biology Commons Scholar Commons Citation Deforte, Shelly, "Intrinsic Disorder Where You Least Expect It: The ncI idence and Functional Relevance of Intrinsic Disorder in Enzymes and the Protein Data Bank" (2016). Graduate Theses and Dissertations. http://scholarcommons.usf.edu/etd/6219 This Thesis is brought to you for free and open access by the Graduate School at Scholar Commons. It has been accepted for inclusion in Graduate Theses and Dissertations by an authorized administrator of Scholar Commons. For more information, please contact [email protected]. Intrinsic Disorder Where You Least Expect It: The Incidence and Functional Relevance of Intrinsic Disorder in Enzymes and the Protein Data Bank by Shelly DeForte A dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy Department of Molecular Medicine College of Medicine University of South Florida Major Professor: Vladimir Uversky, Ph.D. Yu Chen, Ph.D. Robert Deschenes, Ph.D. Sandy Westerheide, Ph.D. Bin Xue, Ph.D. Date of Approval: June 14, 2016 Keywords: intrinsically disordered protein, x-ray crystallography, structural biology, enzyme function Copyright © 2016, Shelly DeForte Table of Contents List of Tables ................................................................................................ iv List of Figures ...............................................................................................
    [Show full text]
  • Acronyms/Abbreviations
    NOAA Coral Reef Information System - Glossary of Terminology Coral Reef Information System Home Data & Publications Regional Portals CRCP Activities Glossary Home / Glossary Home / Glossary of Terminology and Acronyms/Abbreviations Acronyms/Abbreviations A | B | C | D | E | F | G | H | I | J | K | L | M | N | O | P | Q | R | S | T | U | V | W | X | Y | Z | 20CR : 20th Century Reanalysis A | B | C | D | E | F | G | H | I | J | K | L | M | N | O | P | Q | R | S | T | U | V | W | X | Y | Z | A : Adenine A/D : Analog/Digital A3H : Activities and Attractions Association of Hawaii AA : Alien Algae AA : Assistant Administrator AA : Atomic Absorption AA : Awareness and Appreciation AA : Awareness and Appreciation Team (SEFCRI) AAAS : American Association for the Advancement of Science AACL : Aquaculture, Aquarium, Conservation and Legislation http://coralglossaryxml.s3-website-us-west-2.amazonaws.com/print-acronym.html[9/13/2015 3:46:07 PM] NOAA Coral Reef Information System - Glossary of Terminology AAFC : Agriculture and Agri-Food Canada AAG : American Association of Geographers AAL : Average Annual Loss AAPA : American Association of Port Authorities AAPG : American Association of Petroleum Geologists AAR : Amino Acid Racemization AARNet : Australian Academic and Research Network AAS : Alternate Air Source AAS : Atomic Absorption Spectrometry AATAMS : Australian Acoustic Tagging and Monitoring System AAUS : American Academy of Underwater Sciences ABC : Acceptable Biological Catch ABC : ATP Binding Cassette ABE : Autonomous Benthic Explorer
    [Show full text]
  • Ferric Heme As a CO/NO Sensor in the Nuclear Receptor Reverbβ by Coupling 2 Gas Binding to Electron Transfer 3 Anindita Sarkar1, Eric L
    bioRxiv preprint doi: https://doi.org/10.1101/2020.06.22.164806; this version posted June 22, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 1 Ferric Heme as a CO/NO Sensor in the Nuclear Receptor Reverbβ by Coupling 2 Gas binding to Electron Transfer 3 Anindita Sarkar1, Eric L. Carter1, Jill B. Harland2, Amy L. Speelman2,3, Nicolai Lehnert2, and Stephen W. 4 Ragsdale1* 5 1Department oF Biological Chemistry, University oF Michigan, Ann Arbor, Michigan 48109. 6 2Department oF Chemistry and Department of Biophysics, University of Michigan, Ann Arbor, Michigan 48109. 7 3Current address: PaciFic Northwest National Laboratory, Richland, WA 99354. 8 9 Abstract 10 Rev-Erbβ is a nuclear receptor that couples circadian rhythm, metabolism, and inflammation.1-7 11 Heme binding to the protein modulates its function as a repressor, its stability, its ability to bind 12 other proteins, and its activity in gas sensing.8-11 Rev-Erbβ binds Fe3+-heme tighter than Fe2+- 13 heme, suggesting its activities may be regulated by the heme redox state.9 Yet, this critical role of 14 heme redox chemistry in defining the protein’s resting state and function is unknown. We 15 demonstrate by electrochemical and whole-cell electron paramagnetic resonance experiments that 16 Rev-Erbβ exists in the Fe3+ form within the cell essentially allowing the protein to be heme-replete 17 even at low concentrations of labile heme in the nucleus.
    [Show full text]
  • Ferric Heme As a CO/NO Sensor in the Nuclear Receptor Rev-Erbß by Coupling Gas Binding to Electron Transfer
    Ferric heme as a CO/NO sensor in the nuclear receptor Rev-Erbß by coupling gas binding to electron transfer Anindita Sarkara, Eric L. Cartera,1, Jill B. Harlandb, Amy L. Speelmanb,c,2, Nicolai Lehnertb, and Stephen W. Ragsdalea,3 aDepartment of Biological Chemistry, University of Michigan, Ann Arbor, MI 48109; bDepartment of Chemistry, University of Michigan, Ann Arbor, MI 48109; and cDepartment of Biophysics, University of Michigan, Ann Arbor, MI 48109 Edited by John T. Groves, Princeton University, Princeton, NJ, and approved November 19, 2020 (received for review August 6, 2020) Rev-Erbβ is a nuclear receptor that couples circadian rhythm, me- are in low nanomolar concentrations (∼<2.5 nM) (20). Here, we tabolism, and inflammation. Heme binding to the protein modu- focus on the Fe3+/2+ redox couple and how this redox poise lates its function as a repressor, its stability, its ability to bind other contributes to Rev-Erb’s ability to be a gas sensor. The heme- proteins, and its activity in gas sensing. Rev-Erbβ binds Fe3+-heme binding constants for the different redox states of Rev-Erbβ range 2+ 3+ 2+ more tightly than Fe -heme, suggesting its activities may be reg- over 200-fold, from the Fe /thiolate with Kd ∼0.1 nM to Fe with ulated by the heme redox state. Yet, this critical role of heme re- Kd ∼22 nM (15). These Kd values indicate that Rev-Erb would lose dox chemistry in defining the protein’s resting state and function a significant amount of heme if it is reduced to the ferrous form, is unknown.
    [Show full text]
  • Biology Is the Most Powerful Technology on the Planet
    biology is the most powerful technology on the planet WE’RE HIRING ginkgobioworks.com/careers One source. Multiple solutions. Wherever your research takes you, whatever the latest technology, you can rely on our real-industry expertise and high quality, trusted products. Together, we can create long-lasting partnerships that stand the test of time and technology. See what we can do for you at www.idtdna.com. table of contents About ......................................................................................................... 9 Team Map ................................................................................................... 10 Contributors ............................................................................................... 12 Sponsors ..................................................................................................... 18 Exhibitors ................................................................................................... 19 Maps ......................................................................................................... 20 Exterior Hynes Convention Center...................................................... 20 Plaza Level ....................................................................................... 21 Halls A and B ..................................................................................... 22 Second Floor..................................................................................... 23 Third Floor.......................................................................................
    [Show full text]
  • M. Muoio and Daniel P. Kelly Kapoor, Timothy R. Koves, Robert Stevens
    Energy Metabolic Re-Programming in the Hypertrophied and Early Stage Failing Heart: A Multi-systems Approach Ling Lai, Teresa C. Leone, Mark P. Keller, Ola J. Martin, Aimee T. Broman, Jessica Nigro, Kapil Kapoor, Timothy R. Koves, Robert Stevens, Olga R. Ilkayeva, Rick B. Vega, Alan D. Attie, Deborah M. Muoio and Daniel P. Kelly Circ Heart Fail. published online September 18, 2014; Circulation: Heart Failure is published by the American Heart Association, 7272 Greenville Avenue, Dallas, TX 75231 Copyright © 2014 American Heart Association, Inc. All rights reserved. Print ISSN: 1941-3289. Online ISSN: 1941-3297 The online version of this article, along with updated information and services, is located on the World Wide Web at: http://circheartfailure.ahajournals.org/content/early/2014/09/18/CIRCHEARTFAILURE.114.001469 Data Supplement (unedited) at: http://circheartfailure.ahajournals.org/content/suppl/2014/09/18/CIRCHEARTFAILURE.114.001469.DC1.html Permissions: Requests for permissions to reproduce figures, tables, or portions of articles originally published in Circulation: Heart Failure can be obtained via RightsLink, a service of the Copyright Clearance Center, not the Editorial Office. Once the online version of the published article for which permission is being requested is located, click Request Permissions in the middle column of the Web page under Services. Further information about this process is available in the Permissions and Rights Question and Answer document. Reprints: Information about reprints can be found online at: http://www.lww.com/reprints Subscriptions: Information about subscribing to Circulation: Heart Failure is online at: http://circheartfailure.ahajournals.org//subscriptions/ Downloaded from http://circheartfailure.ahajournals.org/ at Loyola University of Chicago on November 3, 2014 Energy Metabolic Re-Programming in the Hypertrophied and Early Stage Failing Heart: A Multi-systems Approach Lai et al: Metabolic Remodeling in Heart Failure Ling Lai, MD, PhD1; Teresa C.
    [Show full text]