Poster Sessions (November 13 & 14)

1. Protein structure and dynamics (I) 1P001 Structure-activity relationships and C-terminal elongation effects of growth-blocking peptide ○Yoshitaka Umetsu1，Tomoyasu Aizawa1,3，Kaori Muto2，Hiroko Yamamoto1，Masakatsu Kamiya1,3，Yasuhiro Kumaki1,3， Mineyuki Mizuguchi2，Makoto Demura1,3，Yoichi Hayakawa4，Keiichi Kawano1,3 1Grad. Sch. of Sci., Hokkaido Univ. 2Fac. of Pharmaceut. Sci., Toyama Univ. 3Grad. Sch. of Life Sci., Hokkaido Univ. 4Fac. of Agr., Saga Univ. 1P002 Structure of tachyplesin I bound to dodecylphosphocholine micelles ○Keitaro Sugita1，Tomoyuki Ohkubo1，Masakatsu Kamiya1,2，Tatsuro Kamijima2，Satoshi Shimamoto1，Fumie Tatami2， Tomoyasu Aizawa1,2，Mineyuki Mizuguchi3，Shun-ichiro Kawabata4，Makoto Demura2，Keiichi Kawano1 1Grad. Sch. of Sci., Hokkaido Univ. 2Grad. Sch. of Life Sci., Hokkaido Univ. 3Fac. of Pharmaceut. Sci., Toyama Univ. 4Dept. of Biol. Grad. Sch. of Sci., Kyushu Univ. 1P003 NMR solution structure of a novel cytokine from the larval integument of the armyworm, Pseudaletia separata ○Masakatsu Kamiya1,2，Shin-ichi Nakatogawa1，Yasunori Oda 3，Tatsuro Kamijima2，Tomoyasu Aizawa1,2，Yasuhiro Kumaki1，Yoichi Hayakawa3，Makoto Demura2，Keiichi Kawano1 1Grad. Sch. of Sci., Hokkaido Univ. 2Grad. Sch. of Life Sci., Hokkaido Univ. 3Dept. of Appl. Biol. Sci., Fac. of Agr., Saga Univ. 1P004 Structure and Dynamics of two Calcium Binding Proteins from Entamoeba histolytica by NMR ○Sourajit M. Mustafi1，Ritu B. Mutalik1，Sulakshna MUkherji1，Kandala V.R. Chary1 1Department of Chemical Sciences, T.I.F.R, Mumbai 1P005 Improved dispersibility and stability of protein samples in solution NMR ○Kazuo Hosoda1，Takeshi Ishii1，Xiang Long1，Tadashi Nakamura 1，Yasuko Iizuka1，Hiroyuki Kogure1，Nobuyuki Nameki1， Toshiyuki Kohno2，Kenji Kubota1，Kaori Wakamatsu1 1Dept. of Biochem. Chem. Engn., Fac. of Engn., Gunma Univ. 2Mitsubishi Kagaku Institute of Life Sciences 1P006 A novel NMR signal assignment method for large and less soluble proteins ○Toshiyuki Kohno1 1Mitsubishi Kagaku Institute of Life Sciences 1P007 Dynamic structure analysis of antibiotic peptide Alamethicin in the lipid bilayers by solid-state NMR ○Daisuke Mishima1，Takashi Nagao1，Daisuke Ishioka1，Akira Naito1 1Sch. Eng., Yokohama Natl. Univ., 1P008 The structures of ion channels with six transmembrane segments revealed by single particle analysis of EM images Toshihiko Ogura 1，Kazuhiro Mio1，○Chikara Sato1 1Neuroscience Research Institute, National Institute of Advanced Industrial Science and Technology (AIST) 1P009 Single particle analysis of purinergic P2X2 receptor ○Kazuhiro Mio1，Yoshihiro Kubo2,3,4，Toshihiko Ogura1，Tomomi Yamamoto2，Chikara Sato1 1Neuroscience Research Institute, National Institute of Advanced Industrial Science and Technology (AIST) 2Division of Biophysics and Neurobiology, Department of Molecular Physiology, National Institute for Physiological Sciences 3COE program for Brain Integration and its Disorders, Tokyo Medical and Dental University Graduate School 4SORST, Japan Science and Technology Corporation 1P010 The fast and robust system to create two-dimensional (2D) average images in single particle analysis ○Masaaki Kawata1，Chikara Sato2 1GTRC, Natl Inst Adv Ind Sci & Technol (AIST) 2NRI, Natl Inst Adv Ind Sci & Technol (AIST) 1P011 A fully automatic 3D reconstruction method using simulated annealing of protein projections ○Toshihiko Ogura1，Chikara Sato1 1Affiliation 1:Neuroscience Research Institute, National Institute of Advanced Industrial Science and Technology (AIST) 1P012 The 3D structure of DNA polymerase/PCNA/DNA complex obtained from single particle EM images ○Kouta Mayanagi1,5，Hirokazu Nishida2，Tsuyoshi Shirai1,5，Yoshizumi Ishino3,5，Kosuke Morikawa4,6 1Nagahama Institute of Bio-Science and Technology 2Hitachi, Ltd. 3Kyusyu Univ. 4Osaka Univ. 5BIRD, JST 6CREST, JST 1P013 Structure of the Whole Cytosolic Region of ATP-Dependent Protease FtsH ○Ryoji Suno1，Daisuke Tsuchiya3,4，Hajime Niwa2，Masasuke Yoshida1，Kosuke Morikawa3,5 1Chem. Res. Lab. Tokyo Inst. of Tech 2Imperial College London 3beri 4Keio Univ. 5Inst. for Protein Research, Osaka Univ. 1P014 Snapshots of tRNA sulfuration via an adenylated intermediate Tomoyuki Numata1，Yoshiho Ikeuchi 2，Shuya Fukai1，Tsutomu Suzuki2，○Osamu Nureki1 1Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology 2Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo 1P015 Crystal structure of the hypothetical protein TTHB029 from Thermus thermophilus HB8 ○Takahito Imagawa1，Hitoshi Iino2，Mayumi Kanagawa2，Akio Ebihara2，Seiki Kuramitsu2,3，Hideaki Tsuge1 1Tokushima Bunri Univ. 2RIKEN Harima Institute at SPring-8. 3Dept of Biology, Osaka Univ. 1P016 Structural analysis of EbhA from Staphylococcus aureus ○Yoshikazu Tanaka1,2，Makoto Kuroda3，Min Yao4，Nobuhisa Watanabe4，Toshiko Ohta 3，Isao Tanaka4 1Dept. of Med. Genome Sci., Grad. Sch. of Front. Sci., The Univ. of Tokyo 2Div. of Biol. Sci., Grad. Sch. of Sci., Hokkaido Univ 3Inst. of Basic Med. Sci., Grad. Sch. of Compreh. Human Sci., Univ. of Tsukuba 4Faculty of Adv. Life Sci., Hokkaido Univ., Sapporo 1P017 Crystal structure and molecular characterization of NEAT domain from Staphylococcus aureus ○Ayuko Suenaga1，Yoshikazu Tanaka1,2,4，Makoto Kuroda3，Kouhei Tsumoto1,2，Toshiko Ohta3，Isao Tanaka5，Izumi Kumagai1 1Dept. of Biomol. Eng., Grad. Sch. of Eng., Tohoku Univ. 2Dept. of Med. Genome Sci., Grad. Sch. of Frontier Sci., the Univ. of Tokyo 3Inst. of Basic Med. Sci., Grad. Sch. of Compreh. Human Sci., Univ. of Tsukuba 4Div. of Biol. Sci., Grad. Sch. of Sci., Hokkaido Univ. 5Faculty of Adv. Life Sci., Hokkaido Univ. 1P018 Structures of binary complex of human BACE1 (β-secretase) ○Hideaki Shimizu1，Asako Tosaki1，Kumi Kaneko1，Takashi Sakurai 1，Nobuyuki Nukina1 1Laboratory for structural neuropathology, Brain Science Institute, RIKEN 1P019 Crystal structure of histidine kinase and response regulator complex ○Seiji Yamada1，Hiroshi Sugimoto1，Miki Kobayashi1,2，Shuji Akiyama1,3，Hiro Nakamura1,4，Yoshitsugu Shiro 1 1RIKEN SPring-8 Center, Harima Inst. 2Grad. Sch. of Life Sci. Univ. Hyogo 3PRESTO, JST 4Grad. Sch. of Int. Sci. Yokohama City Univ. 1P020 Crystal Structure of Wild type Putidaredoxin from Pseudomonas Putida Masayuki Kondo1，Shigeki Matoba1，Kanako Sugiyama2，Sam-Yong Park2，○Hiroshi Hori1 1Dept. of Bioeng., Osaka Univ. 2Protein Design Lab., Yokohama City Univ. 1P021 ADAMs' architecture revealed by VAPs: Molecular mechanism of ectodomain shedding by ADAMs Soichi Takeda1,2，○Tomoko Igarashi1，Kazuhiko Sugata1，Hidezo Mori1，Satohiko Araki3 1Dept Cardiac Physiol, Natl Cardiovas Ctr Res Inst 2Riken Harima Inst 3Sch Biol Sci, Nagoya Univ 1P022 The crystal structure of N-terminal domain of plants NADPH oxidase ○Takashi Oda 1，Kokoro Hayashi2，Chojiro Kojima2，Hiroshi Hashimoto1，Tsutomu Kawasaki2，Ko Shimamoto2，Mamoru Sato1，Toshiyuki Shimizu1 1International Graduate School of Arts and Sciences, Yokohama City University 2Graduate School of Biological Sciences, Nara Institute of Science and Technology 1P023 Structural insight of interaction between proliferating cell nuclear antigen and human TLS polymerases ○Asami Hishiki1，Hiroshi Hashimoto1，Keijiro Kamei2，Eiji OHASHI2，Toshiyuki Shimizu1，Haruo Ohmori2，Mamoru Sato1 1International Graduate School of Arts and Sciences, Yokohama City University 2Institute for Virus Research, Kyoto University 1P024 Structural basis for histone recognition and inhibition mechanism of human peptidylarginine deiminase 4 ○Kyouhei Arita1，Yuan Lu o 2，Paul Thompson2，Toshiyuki Shimizu1，Hiroshi Hashimoto1，Yu ji Hid aka 3，Michiyuki Yamada 1，Mamoru Sato1 1International Graduate School of Arts and Sciences, Yokohama city University 2University of South Carolina 3Faculty of Science and Engineering, Kinki University 1P025 8-oxo-dGTP hydrolysis mechanism in the MutT crystal by kinetic protein crystallography Teruya Nakamura1，○Yuriko Yamagata 1 1Grad. Sch. of Pharmaceut. Sci., Kumamoto Univ. 1P026 An attempt to analyze structural fluctuation in protein crystals using isotropic atomic displacement parameters ○Kazuaki Harata1，Toshihiko Akiba1 1Biological Information Research Center, AIST 1P027 Structual and biochemical studies of MHC class I recognition by human Leukocyte Ig-like receptors Kimiko Kuroki1，Mitsunori Shiroishi1，Mizuho Kajikawa1，Linda Rasubala1，Daisuke Kohda1，○Katsumi Maenaka1 1Div. of Struct. Biol., Med. Inst. of Bioreg., Kyushu Univ. 1P028 Endophilin BAR domain drives membrane curvature by two newly identified structure-based mechanisms Michitaka Masuda1，○Soichi Takeda2,3，Manami Sone1，Takashi Ohki1，Hidezo Mori2，Yuj i Kami ok a 1，Naoki Mochizuki1 1Dept Struct Analysis, Natl Cardiovas Ctr Res Inst 2Dept Cardiac Physiol, Natl Cardiovas Ctr Res Inst 3Riken Harima Inst 1P029 Structure of 6-Pyruvoyl Tetrahydrobiopterin Synthase from Pyrococcus horikoshii OT3: Novel Oligomerisation and Substrate Binding Modes ○Bagautdin Bagautdinov1，Mitsuaki Sugahara2，Naoki Kunishima1 1Advanced Protein Crystallography Research Group , RIKEN SPring-8 Center, Harima Institute 2Structural Biophysics Laboratory, RIKEN SPring-8 Center, Harima Institute 1P030 Homodimeric Crossover Structure of the Human GCSF-Receptor Signaling Complex ○Taro Tamada 1，Eijiro Honjo1，Yoshitake Maeda2，Tomoyuki Okamoto2，Matsujiro Ishibashi3，Masao Tokunaga3，Ryota Kuroki1 1Quantum Beam Sci., Japan Atomic Energy Agency 2Pharm. Research Lab., Kirin Brewery Co. Ltd. 3Faculty of Agri., Kagoshima Univ. 1P031 Mail-in data collection at SPring-8 protein crystallography beamlines ○Nobuo Okazaki1，Kazuya Hasegawa1，Go Ueno2，Yuji Fukumoto2，Hironori Murakami2，Hisanobu Sakai1，Masaki Yamamoto1,2 1SPring-8/JASRI 2SPring-8/RIKEN 1P032 Real-Time SAXS Observation of Assembling-Disassembling Cycles of Cyanobacterial Circadian Clock Proteins ○Atsushi Nohara1，Kazuki Ito2，Yuichiro Maeda 1，Takao Kondo1，Shuji Akiyama3 1Division of Biol. Science, Graduate School of Science, Nagoya University 2RIKEN Harima Institute SPring-8 Center 3PRESTO, Japan Science and Technology Agency 1P033 Small-angle X-ray Scattering Studies on Assembling-Disassembling Complexes of Cyanobacterial Circadian Clock Proteins ○Shuji Akiyama1，Atsushi Nohara2，Kazuki Ito3，Yuichiro Maeda 2，Takao Kondo2 1PRESTO, Japan Science and Technology Agency 2Div. of Biol. Science, Graduate School of Science, Nagoya University 3RIKEN Harima Institute SPring-8 Center 1P034 Conformational analysis of trigger factor and its mutant Junmei Zhou1，Yi Shi 1，○Masaji Shinjo2，Hiroshi Kihara2 1Institute of Biophysics, Academia Sinica 2Department of Physics, Kansai Medical University 1P035 Analysis of secondary structure with wide-angle solution X-ray scattering Takashi Nakagawa1，Hiroshi Kihara2，Shigeru Yanagi1，○Masaki Kojima1 1School of Life Sci., Tokyo Univ. of Pharm. and Life Sci. 2Dept. of Physics, Kansai Med. Univ. 1P036 The Structural Characterization of the Molten Globule States of Cytochrome c Evaluated by Solution X-ray Scattering ○Shigeyoshi Nakamura1，Yasutaka Seki 1，Kunitugu Soda1，Shun-ichi Kidokoro1 1Dept. of Bioeng, Nagaoka Univ. of Technol. 1P037 Hydration related protein dynamics studied by incoherent neutron inelastic scattering ○Hiroshi Nakagawa1，Yasumasa Joti2，Akio Kitao2，Kaoru Shibata1，Nobuhiro Go1，Mikio Kataoka1,3 1QBSD, JAEA 2Lab. of Mol, Des. Tokyo Univ. 3Dept. of Mat. Sci. NAIST 1P038 Molecular simulation study of the origin of non-Gaussian behavior on the elastic incoherent neutron scattering from protein dynamics ○Atsush Tokuhisa1,2，Yasumasa Joti3，Hiroshi Nakagawa4，Akio Kitao3，Mikio Kataoka1,4 1Graduate School of Materials Science, Nara Institute of Science and Technology 2Computational Biology Group, Neutron Biology Research Center, Quantum Beam Science Direction, Japan Atomic Energy Agency 3Center for Bioinformatics, Institute of Molecular and Cellular Biosciences University of Tokyo 4 Neutron Biophysics Group, Neutron Biology Research Center, Quantum Beam Science Direction, Japan Atomic Energy Agency 1P039 Dynamic structures of proteins characterized by networks formed by connecting positively correlated residues in normal mode vibrations ○Hiroshi Wako1，Shigeru Endo2 1Sch. Social Sciences, Waseda Univ. 2Dept. Physics, Sch. Science, Kitasato Univ. 1P040 Normal Mode Analyses of multimeric proteins with symmetry in Dihedral Angle Space ○Shigeru Endo1，Hiroshi Wako2 1Dept. of Phys., Sch. of Science, Kitasato Univ. 2Sch. of Social Scinece, Waseda Univ. 1P041 Structural alignment with Delaunay codes characterizing local structures and structural motifs identified by the alignment ○Shohei Tamura1，Yaemi Teramoto 1，Jiro Katto1，Hiroshi Wako2 1Grad. Sch. of Sci. and Eng., Waseda Univ. 2Sch. of Soc. Sci., Waseda Univ. 1P042 Large-scale Relaxation Phenomena in Complex Elastic Networks: Possible Design Principles of Molecular Machines ○Yuichi Togashi1，Alexander S. Mikhailov1 1Dept. of Phys. Chem., Fritz Haber Inst. of the Max Planck Society 1P043 Determination of hydrogen-atom positions for endoPG using Molecular Dynamics Simulations ○Isao Okazaki1，Minoru Saito1 1Faculty of Science and Technology, Hirosaki University 1P044 Ligand-dissociation increases protein flexibility in trp RNA-binding attenuation protein (TRAP): Molecular dynamics simulation study ○Hidemi Araki1，Sotaro Fuchigami1，Mitsunori Ikeguchi1，Jonathan G. Heddle1，Jeremy R.H. Tame1，Akinori Kidera1 1International Graduate School of Arts and Sciences, Yokohama City Univ. 1P045 Gap compression/extension mechanism of bacterial flagellar hook as the molecular universal joint ○Tadaomi Furuta1,2，Fadel A. Samatey3,4，Hideyuki Matsunami3，Katsumi Imada3,4，Keiichi Namba3,4，Akio Kitao2,5 1Graduate School of Agricultural and Life Sciences, The University of Tokyo 2Core Research for Evolutional Science and Technology, Japan Science and Technology Agency 3Dynamic Nano Machine Project, International Cooperative Research Project, Japan Science and Technology Agency 4Graduate School of Frontier Biosciences, Osaka University 5 Institute of Molecular and Cellular Biosciences, The University of Tokyo 1P046 Collective Coordinate Coupling Analysis of Protein Dynamics ○Akira R. Kinjo1 1Institute for Protein Research, Osaka University 1P047 Analysis of the dynamics of RuvA - RuvB - Holliday junction DNA complex studied by molecular dynamics simulation ○Hisashi Ishida1，Nobuhiro Go1 1Japan Atomic Energy Agency, Quantum Beam Science Directorate 1P048 Flexible docking multicanonical molecular dynamics simulations between factor Xa and its inhibitors ○Narutoshi Kamiya1，Yasushige Yonezawa2，Haruki Nakamura2 1Graduate School of Medicine, Kobe Univ. 2Institute for Protein Research, Osaka Univ. 1P049 A new backbone-torsion-energy term represented by a double Fourier series ○Yoshitake Sakae 1，Yuko Okamoto 2 1QuLiS, Hiroshima Univ. 2Dept. of Physics, Nagoya Univ. 1P050 Extracting dynamical regularity during structural transition ○Hiroshi Teramoto1，Kazuo Takatsuka1 1Department of Basic Science, Graduate School of Arts and Sciences, The University of Tokyo 1P051 Free Energy Landscape Picture and Correlation of Multiple Time Scale Conformational Fluctuations in Protein Allostery ○Kazuhito Itoh1，Masaki Sasai1 1Dept of Computational Science and Engineering, Nagoya Univ. 1P052 Development of a Technique to Dynamically Predict Protein Structures for the Reproduction of Protein Folding Process ○Takeshi N. Sasaki 1，Masaki Sasai1 1Dept. of Comp. Sci. and Eng., Grad. Sch. of Eng., Nagoya Univ. 1P053 A novel method of calculating the volume of the space between two subunits of a protein ○Miki Maeda1，Hiroya Nobori2 1National Institute of Agrobiological Sciences 2Mitsubishi Space Software Co., Inc. 1P054 Calculation of Heat Capacity and Entropy Convergence of BPTI by Using Extended Scaled Particle Theory ○Masayuki Irisa1 1Dept. of Comp. Sci. and Systems Eng., Kyushu Inst. Technology 1P055 QM/MM study on catalytic mechanism of PPIase protein Pin1 ○Yasushige Yonezawa1，Narutoshi Kamiya2，Haruki Nakamura1 1IPR,Osaka Univ. 2CGI,Med.,Kobe Univ. 1P056 Theoretical study on the physicochemical properties of Pumilio RNA-binding domain by quantum chemical calculations ○Ikuo Kurisaki1，Kaori Fukuzawa2,4，Tatsuya Nakano3,4，Hirofumi Watanabe1,4，Shigenori Tanaka1 1Kobe University, Graduate School of Science and Technology 2Mizuho Information & Reseach Institute, Inc. 3Division of Safety Information on Drug, Food and Chemicals, National Institute of Health Sciences 4Japan Science and Thechnology Agency, CREST 1P057 Properties of Residue Contact Occurrence in Denatured Proteins Calculated with the Potential from the Interresidue Average Distances ○Takeshi Kikuchi1 1Dept. Biosci. and Bioinf., Col. Inf. Sci. and Eng., Ritsumeikan Univ. 1P058 Development and evaluation of a system for scoring the local structural segment configuration ○Yoshihide Makino 1，Nobuya Itoh1 1Biotechnology Research Center, Toyama Prefectural University 1P059 Predicting ligand binding sites of uncharacterized protein structure ○Mizuki Morita1，Shugo Nakamura1，Kentaro Shimizu1 1Graduate School of Agricultural and Life Sciences, The University of Tokyo 1P060 Development of a protein tertiary structure prediction server ○Shugo Nakamura1，Mizuki Morita1，Masanori Kakuta1，Kentaro Shimizu1 1Dept. of Biotechnology, The Univ. of Tokyo

2. Protein function (I) 1P061 Spectroscopic and Electrochemical Studies of Pseudoazurin Met16X Variants ○Yuji Obara 1，Rehab F. Abdlhamid1，Hiroshi Hori2，Takamitsu Kohzuma1 1Inst. of Appl. Beam Sci., Ibaraki Univ. 2Grad. Sch. of Eng. Sci., Osaka Univ. 1P062 Aromatic Ring Stacking Interaction in a Blue Copper Protein, Pseudoazurin ○Takayuki Higuchi1，Rehab F. Abdelhamid1，Yoshiko Uchida1，Yu ji Ob ara 1，Takamitsu Kohzuma1 1Ibaraki Univ. Inst. Appl. Beam Sci. 1P063 Comparative analysis of ribosome atomic structures deduced computationally from EM images and X-ray structures ○Yu Tsustsumi1，Atsushi Matsumoto1,2，Kei Yura1,2，Hisashi Ishida1,3 1CREST, JST 2CCSE, Japan Atomic Energy Agency 3QBSD, Japan Atomic Energy Agency 1P064 Comprehensive analysis of the MHCI binding of leukocyte immunoglobulin-like receptors using surface plasmon resonance technique ○Kimiko Kuroki1，Mitsunori Shiroishi1，Yuko Fukunaga1，Daisuke Kohda1，Katsumi Maenaka1 1Div. Struct. Biol., Med. Inst. Bioreg., Kyushu Univ. 1P065 Structural study of mutated FcεRI β chain ITAM by CD spectroscopy. ○Tomoyoshi Terada 1，Koichi Murayama1，Chisei Ra1 1Department of Physiology and Biophysics, Gifu University Graduate School of Medicine 2Division of Molecular Cell Immunobiology and Allergology, Advanced Medical Research Center, Nihon University Graduate School of Medical Science 1P066 Near Native Excited states in DLC8 Dimer : Implications to Cargo Trafficking ○Poluri M. Krishna Mohan1，Ramakrishna V. Hosur1 1Dept. of Chemical Sciences, Tata Institute of Fundamental Research 1P067 Structural and mutational analyses of curculin, a taste-modifying and sweet-tasting protein ○Eiji Kurimoto1，Maiko Suzuki1，Eiko Amemiya1，Yoshiki Yamaguchi 1，Satoru Nirasawa2，Yoshie Kurihara 3，Nobuhisa Shimba4，Ningchun Xu4，Tatumi Kashiwagi 4，Misako Kawai4，Ei-ichiro Suzuki4，Koichi Kato1 1Grad. Sch. Pharm. Sci., Nagoya City Univ. 2National Food Research Institute 3Yokohama National Univ. 4Ajinomoto Co., Inc. 1P068 Fluorescence spectroscopic studies on the conformational changes of thermostabilized Inorganic Pyrophosphatase mutants. Taku Kanai1，Ayumi Maeda 1，Eiichiro Hanafusa1，○Takanori Satoh1 1Fac. of Int.Arts and Sci, Tokushima Univ. 1P069 Observation of GroEL-GroES action by high speed atomic force microscopy ○Daisuke Yamamoto1,2，Masaaki Taniguchi1，Toshio Ando1,2 1Dept. of Physics, Kanazawa Univ. 2JST/CREST 1P070 Single-molecule imaging of chaperonin GroEL-GroES dynamics using high-speed atomic force microscopy ○Masaaki Taniguchi1，Daisuke Yamamoto1,2，Noriyuki Kodera1，Toshio Ando1,2 1Department of Physics, Faculty of Science, Kanazawa University 2JST/CREST 1P071 The allosteric transition of GroEL in the presence of ADP ○Kazunobu Takahashi1，Hokuto Yamada1，Kosuke Maki1，Kunihiro Kuwajima1 1Department of Physics, School of Science, University of Tokyo 1P072 Flexible C-terminal region of GroEL contributes to maintaining the environment of central cavity for proper protein folding ○Koudai Machida1，Akane Okada1，Kunihiro Hongo1，Tomohiro Mizobata1，Yasushi Kawata1 1Dept. Biotech., Fac. Eng. and Grad. Sch. of Biomed. Sci., Tottori University 1P073 Development of a single molecule fluorescence microscope with rapid solution switching system using microfablication technique ○Tomoya Sameshima1，Yukiko Sato2，Taro Ueno1，Takahiro Arakawa2，Yoshitaka Shirasaki2，Shuichi Shoji2，Takashi Funatsu1 1Graduate School of Pharmaceutical Sciences, The University of Tokyo 2Graduate School of Science and Engineering, Waseda University 1P074 Non-native structure of α-lactalbumin-fatty acid complex as an apoptosis induction factor ○Tatsuro Kamijima1，Sato Toshiya2，Kaoru Akimoto2，Tomoyasu Aizawa2，Keiichi Kawano2，Makoto Demura1 1Bioinfo. and Mol. Sci. Course, Div. of Life Science, Grad. School of Life Sci., Hokkaido Univ. 2Biophysical Sciences, Dept. of Biol. Science, Grad. School of Sci., Hokkaido Univ. 1P075 Analysis of complex formation among FEN1, PCNA and 5'-flap DNA by Fluorescence Cross Correlation Spectroscopy ○Hiroshi Sakata1，Makiyo Uchida2，Masataka Kinjo1，Hiroshi Morioka2 1Laboratory of Biophysics, RIES, Hokkaido University 2Faculty of Pharmaceutical Sciences, Hokkaido University 1P076 Molecular basis for recognition of E-cadherin by killer cell-lectin like receptor, KLRG1 ○Seiko Nakamura1，Kimiko Kuroki1，Izuru Ohki1，Kaori Sasaki1，Takuma Maruyama2，Masayuki Ito2，Mitsuhiko Ikura3， Kazuo Yamamoto2，Naoki Matsumoto2，Daisuke Kohda1，Katsumi Maenaka1 1Div. Struct. Biol., Med. Inst. Bioreg., Kyushu Univ., 2Dep. Integrated Biosci., Grad. Sch. Frontier Sci., Tokyo Univ., 3Div. Signal. Biol., Ontario Cancer Inst., Univ. of Toronto, 1P077 Isothermal titration calorimetric and fluorescence quenching studies on the phloem exudate lectin from pumpkin (Cucurbita maxima) ○A. Narahari1，Musti J. Swamy1 1School of Chemistry, Univ. of Hyderabad, Hyderabad-500046 1P078 Characterization of the interaction between Pex3p and Pex19p involved in Peroxisomal membrane protein trafficking ○Yasuhiko Sato1，Hiroyuki Shibata2，Hiroaki Nakano1，Tsuneo Imanaka3，Hiroaki Kato1 1Grad. Sch. Pharm, Kyoto Univ. 2Natl. Cardiovas. Ctr. Res. Inst. 3Dept. of Biol. Chem., Fac.of Pharm. Sci., Univ. of Toyama 1P079 The interaction mechanism between the RING-H2 finger domain of ubiquitn ligase and the ubiquitin conjugating enzyme ○Etsuko Katoh1，Shizue katoh1，Koji Kikuchi1，Yuki Tsunoda1，Cui Hong1，Eiichi Minami1 1National Institute of Agrobiological Sciences 1P080 Bacterial Unsaturated Glycoside Hydrolases as Virulent Factors: Novel Catalytic Reaction Mechanism ○Takafumi Itoh1，Akihito Ochiai2，Bunzo Mikami1，Wataru Hashimoto2，Kousaku Murata2 1Div. of Appl. Life Sci., Grad. Sch. of Agriculture, Kyoto Univ. 2Div. of Food Sci. and Biotech., Grad. Sch. of Agriculture, Kyoto Univ. 1P081 Reaction Mechanism of Arginine Kinase with Substrate Specificity Towards D-arginine ○Kouji Uda1,2，Aiko Matsumoto1，Tomohiko Suzuki1 1Laboratory of Biochemistry, Faculty of Science, Kochi University 2JSPS Research Fellow (PD) 1P082 Substrate recognition system of phosphagen kinases. Importance of amino acid residue 95. ○Kumiko Tanaka1，Tomohiko Suzuki1 1Laboratory of Biochemistry, Faculty of Science, Kochi University 1P083 Peptide fragment of m4 muscarinic receptor activates Gi/o but not Gq Makoto Kubota1，Hideki Kusunoki2，Takeshi Tanaka2，Tomohito Morikawa 1,2，Kazuki Sato3，Hidehito Mukai2，Toshiyuki Kohno2，○Kaori Wakamatsu1 1Dept. of Biological and Chemical Engineering, Gunma Univ. 2Mitsubishi Kagaku Institute of Life Sciences 3Dept. of Environmental Science, Fukuoka Womens Univ. 1P084 Sensitive and precise evaluation of the glycosidase activity for the hydrolysis of several substrates using multi- wavelength BCA method ○Satoshi Souma1，Yuu Saitou1，Shun-ichi Kidokoro1 1Dept. of Bioeng, Nagaoka Univ. of Tech. 1P085 Exploring the Complete Sequence Space at a Hamming Distance One around E. coli Dihydrofolate Reductase: Stability and Activity ○Hisashi Takahashi1，Akiko Yokota1，Tatsuyuki Takenawa1，Munehito Arai1，Yukiko Aruga1，Masahiro Iwakura1 1AIST 1P086 Structure and Function of Dihydrofolate Reductases from Deep-sea Bacteria ○Chiho Murakami1，Eiji Ohmae1，Kunihiko Gekko1，Shin-ichi Tate1，Kaoru Nakasone2，Fumiyoshi Abe3，Chiaki Kato3 1Graduate School of Science, Hiroshima Univ. 2 Faculty of Engineering, Kinki Univ. 3Japan Agency for Marine-Earth Science and Technology 1P087 Function of Trp104 in Human Hematopoietic Prostaglandin D2 Synthase ○Yosuke Urahama1，Yoshiko Uchida1，Yoshihiro Urade 2，Takamitsu Kohzuma1 1Inst. Appl. Beam. Sci., Ibaraki Univ., 2Dept. Mol. Behav. Biol., Osaka Bioscience Inst., 1P088 N-linked protein glycosylation in hyperthermophilic archaeon Pyrococcus furiosus ○Jun Kamishikiryou1，Mayumi Igura1，Katsumi Maenaka1，Daisuke Kohda1 1Div. of Structural Biology, Kyushu Univ. 1P089 ATPase activities of the type III protein export systems ○Fumio Hayashi1，Eri Inobe1，Toru Minamino2,3，Katsumi Imada2,3，Keiichi Namba2,3，Kenji Oosawa1 1Dept. of Nano-Material Systems, Gunma Univ. 2Grad. School of Frontier Biosciences, Osaka Univ. 3Dynamic NanoMachine Project, ICORP, JST 1P090 Excavation of novel peptidyl-prolyl isomeases on the basis of information acquired by comprehensive mutational analysis ○Teikichi Ikura1，Kengo Kinoshita2，Nobutoshi Ito1 1School of Biomedical Science, Tokyo Medical & Dental Univ. 2Institute of Medical Science, Univ. of Tokyo 1P091 Observation of the gradually freezing ice/solution interface on aqueous solution of antifreeze protein ○Rikiya Nakanishi1，Yusuke Morikane1，Yoshimichi Hagiwara2 1Graduate student, Graduate school of Science and Technology, Kyoto Institute of Technology 2Graduate school of Science and Technology, Kyoto Institute of Technology 1P092 Anisotropic growth kinetics of ice crystals affected by interface adsorption of antifreeze Glycoprotein(AFGP) molecules ○Yoshinori Furukawa1，Kousuke Nomura1，Takanori Terasawa 1，Salvador Zepeda1，Yukihiro Uda1，Etsuro Yokoyama2 1ILTS, Hokkaido Univ. 2Comp. C., Gakushuin Univ.

3. Protein folding and misfolding (I) 1P093 A Theoretical Analysis on the Free Energy Changes Associated with Protein Folding ○Takashi Imai 1，Yuichi Harano 2，Masahiro Kinoshita2，Andriy Kovalenko3,4，Fumio Hirata5 1Department of Bioscience and Bioinformatics, Ritsumeikan University 2International Innovation Center, Kyoto University 3National Institute for Nanotechnology, National Research Council of Canada 4Department of Mechanical Engineering, University of Alberta 5Department of Theoretical Studies, Institute for Molecular Science 1P094 New replica exchange method, REST ○Tomoshi Kameda1 1CBRC, AIST 1P095 Coarse-graining of coarse-grained protein models ○Hiromitsu Shimoyama1，Macoto Kikuchi2 1Dept. of Physics, Osaka Univ. 2Cybermedia Center, Osaka Univ. 1P096 Long time folding simulations of a three-stranded antiparallel β-sheet peptide and a ββα-folded peptide ○Tadashi Ando1，Ichiro Yamato1，Naoki Kobayashi2，Masayuki Takeda2 1Department of Biological Science and Technology, Tokyo University of Science 2Department of Information Sciences, Tokyo University of Science 1P097 Folding simulation of a designed zink-finger like protein ○Motonori Ota1，Mitsunori Ikeguchi2，Akinori Kidera2 1GSIC, Tokyo Tech. 2Intntl. Grad. Schl. Arts & Sci., Yokohama C. Univ. 1P098 Structure-based design of folding cooperativity of a de novo Cro protein ○Yasuhiro Isogai1 1RIKEN SPring-8 Center, Harima Institute 1P099 A novel method for predicting the native structure of a protein ○Yuichi Harano1，Roland Roth2，Yuji Sugita3，Mitsunori Ikeguchi4，Masahiro Kinoshita1 1International innovation center, Kyoto Univ. 2Max-Planck institute for Metallforschung, Stuttgart 3Inst. of molecular and cellular biosciences, The Univ. of Tokyo 4International graduate school of arts and sciences, Yokohama City Univ. 1P100 Effect of single amino acid substitutions on kinetic properties of folding studied by a statistical mechanical model of lattice proteins ○Haruo Abe1，Hiroshi Wako2 1Dept. of Natural Sci., Nishinippon Inst. of Tech. 2Sch. of Social Sci., Waseda Univ. 1P101 The role of Ca2+ in the folding of goat α-Lactalbumin ○Katsuaki Tomoyori1，Kimiko Saeki1，Kosuke Maki1，Takashi Nakamura1，Kunihiro Kuwajima1 1Department of Physics School of Science Univ. of Tokyo 1P102 Comparison of burst phase folding intermediates of tear lipocalin and β-lactoglobulin. ○Seiichi Tsukamoto1，Takako Yamasita1，Hideaki Tsuge2，Kazuo Fujiwara1，Kosuke Maki3，Kunihiro Kuwajima3， Masamichi Ikeguchi1 1Dept. of Bioeng., Fac. of Tech., Soka Univ. 2Inst. for Health Sci., Tokushima Bunri Univ. 3Dept. of Phys., Sch. of Sci., Tokyo Univ. 1P103 Identification and characterization of key substructures involved in the early folding events of a (β/α)8-barrel protein ○Satoshi Akanuma 1，Akihiko Yamagishi1 1Dept. of Molecular Biology, Tokyo Univ. of Pharm. and Life Sci. 1P104 Conformational Changes during Apoplastocyanin Folding Observed by Photocleavable Modification and Transient Grating ○Shun Hirota1,2，Yu kari Fu ji mot o 3，Jungkwon Choi4，Naoki Baden4，Noriko Katagiri3，Masako Akiyama3，Rinske Hulsker5， Marcellus Ubbink5，Toshihide Okajima6，Teruhiro Takabe7，Noriaki Funasaki1，Yoshihito Watanabe3，Masahide Terazima4 1Department of Physical Chemsitry, Kyoto Pharmaceutical University 2PRESTO, JST 3Department of Chemistry, Graduate School of Science, Nagoya University 4Department of Chemistry, Graduate School of Science, Kyoto University 5Leiden Institute of Chemistry, Gorlaeus Laboratories, Leiden University 6Institute of Scientific and Industrial Research, Osaka University 7Research Institute, Meijo University 1P105 Equilibrium and kinetics of the folding of canine milk lysozyme ○Hiroyasu Nakatani1，Kosuke Maki1，Kimiko Saeki1，Tomoyasu Aizawa2，Makoto Demura3，Keiichi Kawano2，Kunihiro Kuwajima1 1Department of Physics, Graduate School of Science, University of Tokyo 2Division of Biological Science, Graduate School of Science, Hokkaido University 3Division of Life Science, Graduate School of Life Science, Hokkaido University 1P106 Submillisecond Folding of Escherichia coli Dihydrofolate Reductase Monitored by Time-Resolved Fluorescence Spectroscopy ○Munehito Arai1，Masahiro Iwakura1，C. Robert Matthews2，Osman Bilsel2 1IBRF, AIST 2Dept Biochem Mol Pharm, UMass Medical School 1P107 Helix-rich intermediate of oxidized and reduced proteins on their folding pathway ○Jinsong Li1，Masaji Shinjo1，Yoshitaka Matsumura1，Hiroshi Kihara1 1Department of Physics, Kansai Medical University 1P108 Development of sheath-flow system to observe protein folding events at a single-molecule level ○Kiyoto Kamagata1,2，Masahito Kinoshita1，Yuji Goto1，Satoshi Takahashi1,2 1Inst. Protein Res. 2CREST•JST 1P109 Solvent-tuning collapse and helix formation time scales of λ*6-85 Charles Dumont1，○ Yoshitaka Matsumura2，Seung J. Kim1，Jinsong Li2，Elena Kondrashkina3，Hiroshi Kihara2， Martin Gruebele1,4 1Dept. of Physics, Univ. of Illinois 2Dept. of Physics, Kansai Medical Univ. 3BioCAT at Advanced Photon Source 4Dept. of Chemistry, Univ. of Illinois 1P110 Characterization of conformational rearrangement during the folding process of Staphylococcal nuclease ○Masayoshi Onitsuka1，Hironari Kamikubo1，Yoichi Yamazaki 1，Yasu shi Imamot o 1，Mikio Kataoka1 1Graduate School of Materials Science, Nara Institute of Science and Technology 1P111 Extraction of the Essential Regions of the Dihydrofolate Reductase Sequence by Alanine Insertion Perturbation Analysis ○Mika Umeyama1，Hiironari Kamikubo1，Masahiro Iwakura2，Mikio Kataoka1 1Graduate School of Materials Science, Nara Institute of Science and Technology 2Protein Design Research Group Institute for Biological Resources and Functions National Institute of Advanced Industrial Science and Technology 1P112 Multiple time scales in the folding dynamics of single chain monellin revealed by single molecule measurements ○Akio Maeda1，Masahito Kinoshita1，Kiyoto Kamagata1，Takashi Konno2，Yuji Goto1，Satoshi Takahashi1,3 1Institute for Protein Research, Osaka University 2Faculty of Medical Science, Fukui University 3CREST/JST 1P113 Backbone NMR assignment and hydrogen exchange of goat α-lactalbumin ○Takashi Nakamura1，Emi Yukawa1，Kosuke Maki1，Kunihiro Kuwajima1 1Department of Physics, School of Science, University of Tokyo 1P114 Folding mechanisms of a proline-free variant of staphylococcal nuclease studied by mutagenesis approach. ○Ryuji Takahashi1，Ryuhei Yoshida1，Kosuke Maki1，Kunihiro Kuwajima1 1Department of Physics, Graduate School of Science, the University of Tokyo 1P115 Fast thermal denaturation of the hyperthermophilic protein, ST1625 product ○Shuichiro Goda1,2，Chihiro Morimoto1，Chika Sakai1，Harumi Yoshida1，Haruhiko Sakuraba1，Toshihisa Ohshima1,3 1Fac. of Eng., The Univ. of Tokushima 2Present address: Fac. of Eng., Nagasaki Univ. 3Present address: Fac. of Agr., Kyushu Univ. 1P116 The Assessment of the New Apparatus for the Single Molecule Detection of Protein Folding ○Masahito Kinoshita1，Yu ji Got o 1，Satoshi Takahasi2 1Inst. Protein.Res. 2CREST, JST 1P117 Unfolding and refolding of three CutA1s from P. horikoshii, T. thermophilus, and O. sativa, with unusually high denaturation temperatures ○Masahide Sawano1，Kyoko Ogasahara2，Shizue Katoh3，Etsuko Katoh3，Shigeyuki Yokoyama1,4,5，Katsuhide Yutani1 1RIKEN Spring-8, Harima Inst. 2Inst.Protein Res.,Osaka Univ. 3Nat.Inst. of Agrobiological Sci. 4RIKEN GSC 5Grad. Sch. Sci.,Univ. Tokyo 1P118 H/D exchange characterization of the C-terminal α-helix in the denatured state of pyrrolidone carboxyl peptidase from a hyperthermophile ○Satoshi Iimura1，Taro Umezaki1，Makoto Takeuchi2，Mineyuki Mizuguchi2，Kyoko Ogasahara3，Hiromasa Yagi3，Hideo Akutsu3，Yasuo Noda 1，Shin-ichi Segawa1，Katsuhide Yutani4 1Sch. Sci. & Tech., Kwansei Gakuin Univ. 2Fac. Pharm. Sci., Univ. Toyama 3Inst.Protein Res., Osaka Univ. 4RIKEN SPring8, Harima Inst. 1P119 2SS[6-127, 64-80] variant of lysozyme forms an ordered structure in the anchor region between α- and β-domains in the presence of glycerol ○Mitsunobu Yusa1，Yasuo Noda 1，Hideki Tachibana2，Shin-ichi Segawa1 1Sch. of Sci. and Tech., Kwansei Gakuin Univ. 2Fac. of Sci., Kobe Univ. 1P120 Folding or not folding in the membrane of Escherichia coli ○Kunio Ihara1 1Center for Gene Research, Nagoya University 1P121 Studies of Molten-Globule Structure of Cytochrome c by Detailed Analysis of Solution X-ray Scattering Profiles. ○Yasutaka Seki 1，Shigeyoshi Nakamura1，Katsuaki Inoue2，Shun-ichi Kidokoro1，Kunitsugu Soda1 1Dept. Bioeng., Nagaoka Univ. Technol. 2Exp. Fac. Div., Spring-8, JASRI 1P122 Fragment analysis of the partially unfolded state of equine β-lactoglobulin ○Kanako Nakagawa1，Masamichi Ikeguchi1 1Dept. of Bioeng., Soka Univ. 1P123 TFE-induced conformations of a synthesized oligopeptide originating from the C-terminal α-helix of pyrrolidone carboxyl peptidase ○Taro Umezaki1，Satoshi IImura1，Yasuo Noda 1，Katsuhide Yutani2，Shin-ichi Segawa1 1Sch. Sci. & Tech., Kwansei Gakuin Univ. 2RIKEN SPring8, Harima Inst. 1P124 Stability of helical structure of bee venom melittin ○Yoshinori Miura 1，Hirofumi Sakashita1 1Center for Advanced Instrumental Analysis, Kyushu Univ. 1P125 TFE-induced structures in lysozyme variants lacking some of the disulfide bonds determined by the residue-specific H-D exchange method. ○Naoki Tokunaga1，Yasuo Noda1，Hideki Tachibana2，Shin-ichi Segawa1 1Sch. Sci. & Tech., Kwansei Gakuin Univ. 2Fac. Sci., Kobe Univ 1P126 Effects of glycerol on kinetics of the disulfide bonds regeneration of 2SS-variant of lysozyme, 2SS[6-127, 64-80] ○Kazuhiro Matsuo1，Kunihiko Okamoto1，Yasuo Noda1，Hideki Tachibana2，Shin-ichi Segawa1 1Sch. Sci. & Tech., Kwansei Gakuin Univ. 2Fac. Sci., Kobe Univ. 1P127 Observation of structure formation in the oxidative refolding of ribonuclease A ○Fumio Kumakura1，Mitsumasa Yoneda1，Michio Iwaoka1 1Department of Chemistry, School of Science, Tokai University 1P128 Role of an off-barrel strand for the structural stabilization of equine β-lactoglobulin ○Yoshiteru Yamada 1，Miwa Wakabayashi1，Kanako Nakagawa1，Kouta Nakayama1，Kazuo Fujiwara 1，Masamichi Ikeguchi1 1Dept. of Bioinfo., Soka Univ. 1P129 Difference of folding properties between natural and de novo Cro proteins ○HIroshi Imamura1，Yasuhiro Isogai2，Minoru Kato1 1Dept. of Appl. Chem., Ritsumeikan Univ. 2Bio-metal Science Lab. RIKEN Harima institute/Spring8 1P130 Effect of Pressure and temperature on the secondary structure of bovine β-lactoglobulin ○Shinko Nakamura1，Minoru Kato1 1Dept. of Appl. Chem., Ritsumeikan Univ. 1P131 Detection of substructural unfolding of SNase by intermolecular force microscopy. ○Akihiro Fukagawa1，Michio Hiroshima2，Kunihiro Kuwajima3，Makio Tokunaga1,2,4 1Structural Biology Center, National Institute of Genetics. 2Research Center for Allergy and Immunology, RIKEN. 3Department of Physics, Graduate School of Science, University of Tokyo. 4Department of Genetics, School of Life Science, The Graduate University for Advanced Studies.

4. Protein engineering 1P132 Structural Diversity of Protein Segments Follows a Power-law Distribution ○Shinya HONDA1，Yoshito SAWADA 1 1National Institute of Advanced Industrial Science and Technology (AIST) 1P133 A method to evaluate heme-binding affinity on the protein theoretical model ○Yukiko Takai1，Motonori Ota2，Yasuhiro Isogai3 1Grad. Sch. of Biosci & Biotechnol., Tokyo Tech. 2GSIC, Tokyo Tech. 3RIKEN Harima Institute 1P134 Photo-switching Calmodulin modified by Photochromic Molecules ○Hideki Shishido1，Masafumi Yamada1，Daichi Shimomura2，Kazunori Kondo2，Shinsaku Maruta1 1Div. of Bioeng., Grad. sch. of Eng., Soka Univ. 2Dep. of Bioinfo., Fac. of Eng., Soka Univ. 1P135 Optimization of Protein Trans-splicing Reaction Conditions ○Tetsuya Kamioka 1，Minako Tawa1，Shihori Sohya1，Yutaka Kuroda1 1Dept of Biotechnology and Life Science, Faculty of Engineerig, Tokyo University of Agriculture and Technology 1P136 Crystal Lattice Engineering: Crystallization and Structure Determination of Human RNase 1 Mutants with a Hydrophobic Interface of Leucines ○Hidenori Yamada1，Kohei Miyata1，Shinya Fujiki1，Masaru Tano1，Masayuki Moriya1，Takeshi Ino1，Megumi Kosaka1， Hiroko Tada1，Junichiro Futami1，Mamoru Yamanishi1，Hiroshi Yamaguchi2，Masaharu Seno1，Takashi Nomoto1，Tomoko Hirata1，Motonobu Yoshimura1，Eijiro Honjo3，Taro Tamada 3，Ryota Kuroki3 1Graduate School of Nat. Sci. Technol, Okayama Univ. 2School of Sci. Technol., Kwansei Gakuin Univ. 3Neutron Sci. Res. Center, Japan Atomic Energy Res. Inst. 1P137 Computational prediction of T cell epitope peptides for designing hepatitis C virus vaccine. ○Keiko Udaka1，Toshie Mashiba 2，Tomoya Miyakawa3，Tsutomu Osada3 1Dept. of Immunology, Kochi Medical School 2Dept. of Gastroenterology and Metabology, Graduate School of Medicine, Ehime Univ. 3Dept. of Bioinformatics Business Promotion, NEC corporation 1P138 A method for producing of rabbit monoclonal antibody from spleen and bone marrow B cells using yeast display system ○Masatoshi Nakagawa1，Takachika Azuma1 1RIBS, Tokyo Univ. of Science 1P139 BmNPV expression system; Application to human natural killer cell receptors ○Mizuho Kajikawa1，Kaori Sasaki1，Kimiko Kuroki1，Tomoko Motohashi2，Tsukasa Shimojima2，Enoch Y. Park3，Daisuke Kohda1，Katsumi Maenaka1 1Div. Struct. Biol., Med. Inst. Bioreg., Kyushu Univ. 2Nat. Inst. Genetics 3Dept. Appl. Biol. Chem., Shizuoka Univ. 1P140 A novel expression system for high-level recombinant protein production in Escherichia coli ○Eri Hojo1，Masakatsu Kamiya1,2，Tomoyasu Aizawa1,2，Mitsuhiro Miyazawa3，Yu suk e Kat o 3，Makoto Demura1,2，Keiichi Kawano1 1Grad. Sch. of Sci., Hokkaido Univ. 2Grad. Sch. of Life Sci., Hokkaido Univ. 3Dept. of Dev. Biol., Natl. Inst. of Agrobiol. Sci. 1P141 Preparation of Ultra Fine Cobalt Ferrite by Chemical Coprecipitation Method and Trial of Encapsulation in Iron Storage Protein Ferritin ○Masafumi Shimizu1，Toshihiko Sato1，Makoto Tsuruoka1 1Tokyo univ. of Tech. 1P142 Site-selective deposition of ferritin on the patterned silicon substrates ○Akira Yamazaki1，Hiroko Fukano1，Hideyuki Yoshimura1 1Dept. of Physics, Meiji University 1P143 LAHTH: A designed helix-turn-helix protein with high thermal stability ○Taku Matsuba 1，Naoki Umeda1，Satoshi Akanuma1，Akihiko Yamagishi1 1Dept. of Molecular Biology, Tokyo Univ. Pham. and Life Sci. 1P144 Hyperthermophilic translation system in the common ancestor: Analysis of ancestral mutants of GlyRS of the Thermus thermophilus. ○Hideaki shimizu1，Shin-ichi Yokobori1，Takatoshi Ohkuri1,2，Takashi Yokogawa3，Kazuya Nishikawa3，Akihiko Yamagishi1 1Department of Molecular Biology, Tokyo University of Pharmacy and Life Science 2Present address: Gradurate Scool of Pharmaceutical Sciences, Kyushu University 3Department of Biomolecular Science, Faculty of Engineering, Gifu University 1P145 Cold adaptation of the thermophilic enzyme 3-isopropylmalate dehydrogenase from Sulfolobus tokodaii ○Michika Sasaki1，Mayumi Uno1，Keiko Watanabe1，Akihiko Yamagishi1 1 Dept. of Molecular Biology, Tokyo Univ. of Pharmacy and Life Science 1P146 Attempt to understand the information encoded in the amino acid sequence of photoactive yellow protein by the simplification of sequence ○Kumiko Shirai1，Yoichi Yamazaki 1，Hironari Kamikubo1，Yasushi Imamoto1，Mikio Kataoka1 1Graduate School of Materials Science, Nara Institute of Science and Technology 1P147 2-dimensional crystallization of mutant bacterioferritin ○Yuka Takasaki1，Motoi Noguchi1，Shigeki Takeda1 1Gunma University 1P148 Study of expression of PSD-95 in neuron-glia mixed culture cells using adenovirus ○Atsuo Miyazawa1，Hyeji Kim1，Yuko Fukunaga1 1Structural Physiology Research Group, RIKEN SPring-8 Center

5. Heme proteins 1P149 Resonance Enhancement of the νFe=O Raman Mode upon Red Excitation ○Kenichiro Ikemura1，Masahiro Mukai2，Hideo Shimada2，Kyoko Shinzawa-Itoh1，Shinya Yoshikawa1，Takashi Ogura1 1Grad. Sch. of Life Sci., Univ. of Hyogo 2Sch. of Med., Keio Univ. 1P150 Spectroscopic Studies of the Putative Heme-binding Domain of Heme-regulated eIF2αKinase ○Kyoko Hirai1，Marketa Miksanova1，Jotaro Igarashi1，Toru Shimizu1 1Institute of Multidisciplinary Research for Advanced Materials, Tohoku University 1P151 Functional studies of a heme-based sensor phosphodiesterase, EcDOS ○Atsunari Tanaka1，Hiroto Takahashi1，Toru Shimizu 1 1Institute of Multidisciplinary Research for Adbanced Materials, Tohoku Univercity 1P152 Effect of SNPs on the Drug Binding and Metabolizing Properties of Human CYP2C9 Tadayuki Uno1，○Noritsugu Ueda1，Yusuke Kagawa1,2，Ryosuke Watanabe2，Yoshikazu Tomisugi1，Hatsuo Maeda1 1Osaka University 2Kumamoto University 1P153 Effects of SNPs on the Drug Binding and Metabolizing Activity of CYP1A2 Tadayuki Uno1，○Masafumi Egawa1,2，Shin-ichi Murata2，Akihide Tsujimoto1，Yoshikazu Tomisugi1，Hatsuo Maeda1 1Osaka Unvi. 2Kumamoto Univ. 1P154 Resonance Raman Studies of CYP2C19 and Its Complexes with Substrates Sayed M. S. Ali1,2，Yukiko Oyama2，Yoshinobu Ishikawa2，Yoshikazu Tomisugi1，Hatsuo Maeda1，○Tadayuki Uno1 1Graduate School of Pharmaceutical Sciences, Osaka University 2Graduate School of Pharmaceutical Sciences, Kumamoto University 1P155 Spectroscopic Studies of Neuronal PAS Protein 2 (NPAS2), a Transcription Activator Protein Associated with Circadian Rhythms ○Naoki Hikage1，Jotaro Igarashi1，Emiko Sato1，Ikuko Sagami2，Toru Shimizu1 1Institute of Multidisciplinary Research for Advanced Materials, Tohoku University 2Grad. Sch. of Agr., Kyoto Pref. Univ. 1P156 Thermodynamic analysis of cofactor binding to the reductase domain of neuronal nitric oxide synthase ○Ikuko Sagami1，Ryuhei Sanae1，Fumiaki Kurokawa1，Yusuke Izutani1，Masayuk Oda1 1Dept. of Biofunction Chemistry, Grad. Sch. of Agri, Kyoto Pref. Univ. 1P157 Exploring the Binding Conformations of Bulkier Dipeptide Amide Inhibitors in Constitutive Nitric Oxide Synthases ○Jotaro Igarashi1,3，Huiying Li1，Mack L. Flinspach III1，Joumana Jamal1，Weiping Yang 1，José Antonio Gómez-Vidal2， Elizabeth A Litzinger2，Hui Huang2，Richard B. Silverman2，Thomas L. Poulos1 1Dept. of Mol. Biol. & Biochem., Univ. California, Irvine 2Dept. of Chem., Northwestern Univ. 3Inst. of Multidisciplinary Res. for Adv. Mat., Tohoku Univ. 1P158 Crystal structure of the heme oxygenase complexed with α-meso hydroxyheme, a high reactive intermediate. ○Masaki Unno1，Masao Ikeda-Saito1 1Inst. of Multidisciplinary Research for Advanced Materials, Tohoku Univ. 1P159 EPR Characterization of the heme complex of a higher plant heme oxygenase-1 ○Tomohiko Goya1，Xuhong Zhang2，Tadashi Yoshida2，Catharina_T. Migita1,3 1The United Graduate School of Agricultural Sciences, Tottori University 2Yamagata University School of Medicine 3Department of Biological Chemistry, Facultyof Agriculture, Yamaguchi University. 1P160 Effect of the aromatic amino-acid mutation on the heme catabolism of rat heme oxygenase-1 ○Satoko Togashi1，Xuhong Zhang2，Tadashi Yoshida2，Catharina_T. Migita1 1Department of Biological Chemistry, Yamaguchi University 2Yamagata University School of Medicine 1P161 Substrate Binding and Metabolizing Properties of Indoleamine 2,3-Dioxygenase Tadayuki Uno1 ，○Mai Ushida1 ，Akemi Matsuura2 ，Chikako Nakayama2 ，Tomoko Hosokawa2 ，Yoshikazu Tomisugi 1，Yoshinobu Ishikawa2，Hatsuo Maeda1 1Osaka University 2Kumamoto University 1P162 Exogenous Ligand Binding Mechanism of Cytoglobin Tadayuki Uno1，○Kaori Kukino1,2，Hatsuo Maeda1，Yoshikazu Tomisugi1，Yoshinobu Ishikawa2，Norifumi Kawada3， Katsutoshi Yoshizato4，Hitomi Sawai5，Yoshitsugu Shiro5 1Osaka Univ. 2Kumamoto Univ. 3Osaka City Univ. 4Hiroshima Univ. 5RIKEN Harima Institute/SPring-8 1P163 Roles of Cysteine Residues in the Gas Binding to Human Neuroglobin Tadayuki Uno1，○Leila Hafsi1,2，Hatsuo Maeda1，Hiroko Tsutsumi2，Daisuke Ryu2，Yoshikazu Tomisugi1，Yoshinobu Ishikawa2，Hatsuo Maeda1 1Osaka Univ. 2Kumamoto Univ. 1P164 Analysis of Multi-Frequency and High-Field EPR Spectra for Powder and Single-Crystal of Mn(III)Mb with Integer Spin (S = 2) ○Masaki Horitani1，Haruhiko Yashiro2，Masayuki Hagiwara2，Hiroshi Hori1 1 Dept. of Bioeng., Osaka Univ 2KYOKUGEN, Osaka Univ 1P165 Ancestral myoglobins: amino acid sequences, syntheses and oxygen-binding properties ○Manabu Ishida1，Atsushi Yasuda2，Iori Yamasaki2，Yu ta M u rakami 3，Motonori Ota4，Yasuhiro Isogai5，Kiyohiro Imai1,6 1Research Center for Micro-Nano Technol., Hosei Univ. 2Dept. of Materials Chem., Grad. Sch. of Eng., Hosei Univ. 3Dept. of Materials Chem., Fac. of Eng., Hosei Univ. 4Dept. of Biosci. and Biotechnol., GSIC Center, Tokyo Inst. of Technol. 5Biometal Sci. Lab., RIKEN, Harima Inst./SPring-8 6Dept. of Frontier Biosci., Fac. of Eng., Hosei Univ. 1P166 Dynamics of Hemoglobin in Fixed Quaternary Structure ○Atsushi Inagaki1，Yasuhisa Mizutani2 1Grad. Schl. of Sci. and Tech., Kobe Univ. 2Grad. Schl. of Sci., Osaka Univ. 1P167 Molecular dynamics simulations of a hemoglobin in water for 45 ns on the earth simulator ○Minoru Saito1，Isao Okazaki1 1Faculty of Science and Technology, Hirosaki University 1P168 Oxygen Binding Properties and Dimer-Tetramer Equilibrium of βW37 Mutants of Human Hemoglobin A ○Masako Nagai1,2，Yayoi Aki-Jin1，Kiyohiro Imai1,2，Fumio Arisaka3，Yukifumi Nagai 1 1Research Center for Micro-Nano Technology, Hosei Univ. 2Dept. of Frontier Bioscience, Faculty of Engineering, Hosei Univ. 3Dept. of Biomolecular Processing, Graduate School of Bioscience and Biotechnology, Institute of Technology 1P169 Over-expression of Recombinant Human Hemoglobin Tadayuki Uno1，○Ishimaru Takefumi1,2，Sohtaro Takigawa1，Yohei Hoashi 3，Toshiya Kai 3，Yoshikazu Tomisugi1，Yoshinobu Ishikawa2，Hatsuo Maeda1 1Osaka Univ. 2Kumamoto Univ. 3Nipro 1P170 Coordination States of E7- or F8-Tyrosine Residue and Quaternary Structure of Five Methemoglobin M Variants: A 244-nm Excited UVRR Study ○Yayoi Aki-Jin 1，Masako Nagai1,2，Michihiko Aki3，Minoru Kubo3，Kiyohiro Imai1,2，Teizo Kitagawa3 1Research Center for Micro-Nano Technology, Hosei Univ. 2Dept. of Frontier Bioscience, Faculty of Engineering, Hosei Univ. 3Okazaki Institute for Integrative Bioscience, National Institutes of Natural Sciences 1P171 Oxygen binding properties of hemoglobins from diving mammals ○Megumi Koyanagi1，Shigenori Nagatomo2，Yoshihiro Fujise3，Tadasu Yamada4，Hajime Mita2，Yasuhiko Yamamoto2， Kiyohiro Imai1 1Dept. of Materials Chem., Grad. Sch. of Eng., Hosei Univ. 2Dept. of Chem., Univ. of Tsukuba 3The Institute of Cetacean Research 4Independent Administrative Institution National Museum 1P172 Determination of amino acid residues, which might be responsible for Root effect of tuna Hb, by site-directed mutagenesis ○Hiroshi Mitsui1，Gentaro Miyazaki1，Hiroshi Hori1 1Dept. of Bioeng., Osaka Univ. 1P173 The Effect of Solvation on the Allosteric Properties of the Bluefin Tuna Hemoglobin ○Tomotaka Kenjo1，Antonio Tsuneshige1，Gentaro Miyazaki2，Kiyohiro Imai1 1Department of Materials Chemistry, Graduate School of Engineering, Hosei University 2Department of Biophysical Engineering, Faculty of Engineering, Osaka University 1P174 Labeling of iron-sulfur protein with a fluorescent dye to detect its conformation change in the cytochrome bc1 complex ○Toshiaki Miki 1，Shun Sakamoto1,2，Hiroaki Yokota1，Yoshie Harada 1,3 1Tokyo Metropolitan Insti. of Med. Sci. 2Dep. of Applied Chem. Tokyo Uni. of Sci. 3CREST, JST 1P175 Determination of the three-dimensional structure of respiratory chain cytochrome c oxidase by electron cryo- microscopy ○Ryosuke Teranishi1，Daisuke Kakihisa1，Hiroshi Fujishima1，Junshi Sakamoto1，Takuo Yasunaga1 1Department of Bioscience and Bioimformatics, Faculty of Computer Science and Systems Engineering, Kyusyu Institute of Technology 1P176 ATR-FTIR Characterisation of the Ferryl Intermediates of Cytochrome c Oxidase ○Masayo Iwaki1，Anne Puustinen2，Mårten Wikström2，Peter R. Rich1 1Glynn Laboratory of Bioenergetics, Department of Biology, University College London 2Helsinki Bioenergetics Group, Institute of Biotechnology, University of Helsinki 1P177 Lipid movements in bovine heart cytochrome c oxidase depending on crystal packing ○Hiroshi Aoyama1，Kyoko Shinzawa-Itoh2，Tomoko Maeda 2，Tomitake Tsukihara3，Shinya Yoshikawa2 1RIKEN Harima Institute 2Dept. of Life Science, Univ. of Hyogo 3Institute for Protein Research, Osaka Univ. 1P178 X-ray structural analysis of the metal binding site on the subunit III of bovine cytochrome c oxidase ○Kazumasa Muramoto1，Sayaka Inaoka1，Kyoko Shinzawa-Itoh1，Tomoko Maeda1，Tomitake Tsukihara 2，Shinya Yoshikawa 1 1Department of Life Science, University of Hyogo 2Institute for Protein Research, Osaka University 1P179 X-ray structural analysis of the ligand-bound conformation of bovine cytochrome c oxidase in the reduced state ○Maki Tanuguchi1，Kazumasa Muramoto1，Kyoko Shinzawa-Itoh1，Tomoko Maeda1，Tomitake Tsukihara 2，Shinya Yoshikawa 1 1Department of Life Science, University of Hyogo 2Institute for Protein Research, Osaka University 1P180 Effect of X-ray induced photoreduction on bovine cytochrome c oxidase ○Hiroyuki Nabekura1，Kazumasa Muramoto1，Kyoko Shinzawa-Itoh1，Tomoko Maeda 1，Tomitake Tsukihara2，Shinya Yoshikawa 1 1Department of Life Science, University of Hyogo 2Institute for Protein Research, Osaka University 1P181 Cancel

1P182 Theoretical Investigation into Proton Transfer Mechanism Involving Peptide Bonds ○Katsumasa Kamiya1，Mauro Boero1,2，Masaru Tateno1,2，Kenji Shiraishi2，Atsushi Oshiyama1,2 1Center for Computational Sciences, Univ. of Tsukuba 2Graduate School of Pure and Applied Physics, Univ. of Tsukuba 1P183 Mutational analyses of the proton pumping H-pathway of bacterial cytochrome c oxidase Seigo Kakiuchi1，Yoshinori Yukutake1，Kunitoshi Shimokata1,2，Yu ku e Kat ayama 1,2，Makoto Suematsu1，Tomitake Tsukihara3， Shinya Yoshikawa4，○Hideo Shimada1 1Dept. of Biochem. Integrative Med. Biol., Sch. of Med., Keio Univ. 2JBIC 3Inst. of Protein Res., Osaka Univ. 4Sch. of Sci. Univ. of Hyogo 1P184 Proton pumping activity of cytochrome c oxidase is essential for mitochondrial biogenesis in HeLa cells, but not the cell growth ○Kunitoshi Shimokata1,2，Yoshinori Yukutake1，Yukie Katayama 1,2，Makoto Suematsu1，Tomitake Tsukihara3，Shinya Yoshikawa 4，Hideo Shimada1 1Sch. of medicine, Keio Univ. 2JBIC 3Inst. of Protein Reserch, Osaka Univ. 4Dept. of life science, Univ.of Hyogo

6. Macromolecular assembly 1P185 Interactions between Vibrio phage KVP40 gp5 and gene product of ORF334, T4 gp27 homologue Mai Nemoto1，○Shuji Kanamaru1，Fumio Arisaka1 1Department of Biomolecular Engineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology 1P186 Role of the T3S4 Domain of the Hook-Length Control Protein FliK in Export Specificity Switching of the Flagellar Type III Export Apparatus ○Tohru Minamino1,2，Hedda F. Ferris3，Nao Moriya1,4，May Kihara3，Keiichi Namba1,2 1Grad. Sch. of Fron. Biosci., Osaka Univ. 2Dynamic Nano. Proj., ICORP, JST 3Dept. of MB & B, Yale Univ. 4JSPS Research Fellow DC 1P187 Role of the N-terminal disordered region in Salmonella hook protein FlgE in its export and self-assembly ○Nao Moriya1,2，Tohru Minamino1,3，Hedda U. Ferris4，May Macnab4，Keiichi Namba1,3 1Graduate School of Frontier Biosciences, Osaka University 2JSPS Research Fellow DC 3Dynamic NanoMachine Project • ICORP • JST 4Department of Biophysics and Biochemistry, Yale University 1P188 Reconstitution of V1 complex of Thermus thermophilus V-ATPase revealed that ATP binding to the A subunit is crucial for V1 formation ○Hiromi Imamura1，Saeko Funamoto2，Masasuke Yoshida2,3，Ken Yokoyama2 1Inst. Sci. Indust. Res., Osaka Univ. 2ATP System Project, JST 3Chem. Res. Lab., Tokyo Inst. Tech. 1P189 Thermal stability and structural feature of actinidain-hydrolyzed collagen self-assembly ○Saori Kunii1，Michiko Shibano2，Takuya Saito1，Koichi Morimoto1 1Grad. School of Biotechnological Science, Kinki University 2Dept. of Microbiology, Kinki University School of Medicine 1P190 Toward creating metal binding helix fiber ○Mika Takahashi1，Toshiaki Takei 1,2，Kin-ichiro Miura2,3，Atsuo Tamura1 1Graduate School of Science and Technology, Kobe Univ. 2Institute for Biomolecular Science, Gakushuin Univ. 3Research Institute, Chiba Institute of Technology. 1P191 Molecular design of functional peptide nanotube ○Aya Kidowaki1，Atsuo Tamura1 1Graduate School of Science and Technology, Kobe University 1P192 Involvement of the molecular chaperone HtpG in the degradation and re-assembly of phycobilisomes in cyanobacteria ○Hitoshi Nakamoto1，Naoki Okamoto1，Kazuki Miyagi1，Takeshi Sato1 1Dept. of Biochemistry and Molecular Biology, Saitama Univ. 1P193 Application of polarized IR-microscope spectroscopy and solid-state NMR to structural analysis of protein fibers ○Ohki Kambara1，Masanori Kiyozono1，Mika Takahashi1，Kengo Daidoji2，Akira Naito2，Toshiaki Takei 1,3，Kin-ichiro Miura3,4，Atsuo Tamura1 1Graduate School of Science and Technology, Kobe Univ. 2Graduate School of Engineering, Yokohama National Univ. 3Institute for Biomolecular Science, Gakushuin Univ. 4Chiba Institute of Technology 1P194 Multiple protein docking guided by low-resolution image of complex using Gaussian mixture model ○Takeshi Kawabata1,3，Kei Yura2,3 1Nara Institute of Science and Technology 2Japan Atomic Energy Agency 3JST CREST 1P195 Crystal structure of infectious bursal disease virus VP2 subviral particle: Implications in virion assembly and immunogenicity ○Tzu-Ping Ko1，Cheng-Chung Lee1，Chia-Cheng Chou1，Masato Yoshimura2，Min-Ying Wang3，Andrew H.-J. Wang1 1Institute of Biological Chemistry, Academia Sinica, Taipei 2Institute for Protein Research, Osaka University, Osaka 3Graduate Institute of Biotechnology, National Chung-Hsing University, Taichung 1P196 Purification of the Type III Secretion Machineries of Vibrio parahaemolyticus ○Jin Yagasaki1，Seiji Kojima1，Tetsuya Iida2，Michio Homma1 1Div. of Biological Science, Nagoya. Univ. 2Dept. of Bact. Infections, Research Inst. for Microb. Disieases, Osaka Univ.

7. Nucleic acid binding proteins 1P197 RNA recognition mechanism of the Human Immunodeficiency Virus Type-2 Nucleocapsid Protein ○Takashi Matsui1，Hiroshi Endoh1，Emi Miyauchi1，Hiroyoshi Komatsu2，Takeshi Tanaka3，Toshiyuki Kohno3，Kazuki Sato4，Yoshio Kodera 1,5,6，Tadakazu Maeda1,5 1Dept. of Physics, Sch. of Sci., Kitasato Univ. 2Dept. of Immunol., Sch. of AHS., Kitasato Univ. 3Mitsubishi Kagaku Inst. of Life Sci. (MITILS) 4Sch. of Human Environmental Sci., Fukuoka Women's Univ. 5Center of Disease Proteomics, Sch. of Sci., Kitasato Univ. 6Clinical Proteomics Research Center, Chiba University Hospital 1P198 Reaction mechanism of bacterial Glu-tRNAGln-dependent amidotransferase ○Akiyoshi Nakamura1，Min Yao1，Sarin Chimnaronk1，Naoki Sakai1，Isao Tanaka1 1Fac. of Adv. Life Sci, Hokkaido Univ. 1P199 Structure of Archaeal Translation Initiation Factor aIF2βγ complex: Implication of the β subunit in GTPase Activation ○Masaaki Sokabe1，Min Yao1，Naoki Sakai1，Shingo Toya1，Isao Tanaka1 1Faculty of Advanced Life Science, Hokkaido Univ. 1P200 Crystal structure of Pyrococcus horikoshii arginyl-tRNA synthetase complexed with tRNAArg and ATP analogue ○Tomomi Sumida1,2，Tatsuo Yanagisawa2，Yuk i e M ori 1，Shigeyuki Yokoyama2,3，Michiko Konno1 1Dept. Chem., Ochanomizu Univ. 2RIKEN Genomic Sciences Center, 3Dept. Biophys. and Biochem., Grad. Sch. Sci., Univ. of Tokyo, 1P201 Analysis of Aquifex MnmC2 tRNA modification enzyme for two codon set of genetic code. ○Madoka Nishimoto1，Rie Shibata1，Emiko Fusatomi1，Takaho Terada1,2，Mikako Shirouzu1,2，Yoshitaka Bessho1,2， Shigeyuki Yokoyama1,2,3 1RIKEN GSC 2RIKEN HARIMA-SP8 3Grad. Sch. Sci. Univ. Tokyo 1P202 Computational analyses of mechanisms of large conformational changes for switching of distinct catalytic reactions of an enzyme Cong Yulong1，○Yohsuke Hagiwara1,2，Masaru Tateno2 1Department of Bioscience and Biotechnology, Tokyo Institute of Technology 2Center for Computational Sciences, University of Tsukuba 1P203 Structure of CO-sensing transcription regulator CooA bound to exogenous ligand imidazole ○Hirofumi Komori1,2，Sayaka Inagaki3，Shiro Yoshioka3，Shigetoshi Aono3，Yoshiki Higuchi1,2 1Dept. of Life Sci., Univ. of Hyogo 2RIKEN Harima Inst. 3Okazaki Inst.for Integrative Biosci. 1P204 The switch of transcription induced by UV-damaged DNA ○Takashi Miyamoto1，Motoki Susa1,2,3，Nobuo Shimamoto1,2 1School of Life Science, SOKENDAI 2Structural Biology Center, National Institute of Genetics 3Present: Management Center of Intellectual Property, Hokkaido Univ. 1P205 The closed structure of an archaeal DNA ligase from Pyrococcus furiosus ○Hirokazu Nishida1，Shinichi Kiyonari2，Yoshizumi Ishino2，Kosuke Morikawa3 1Central Research Labratory, Hitachi Ltd. 2Dept. of Genetic Resources Technology, Kyushu University 3Department of Structural Biology, Biomolecular Engineering Research Institute (BERI) 1P206 Free Energy Landscape for Interactions between DNA Bases and Protein Side Chains ○Tomoki Yoshida 1,2，Hideo Doi1,2，Misako Aida1,2，Hidetoshi Kono3，Shaji Kumar4，Michael M. Gromiha4，Akinori Sarai6 1Dept. of Chem., Grad. Sch. of Sci. Hiroshima Univ. 2Center for Quantum Life Sciences (QuLiS) 3Japan Atomic Energy Agency (JAEA) 4Advanced Tech. Inst. 5Comput. Biol. Res. Cen. (CBRC) 6Dept. of Biosci. and Bioinfo., Kyushu Inst. of Tech. 1P207 Classification of Protein-DNA Complexes Based on Structural Descriptors Ponraj Prabakaran2，Joerg G. Siebers1，Shandar Ahmad3，Michael M. Gromiha4，Maria G. Singarayan5，○ Akinori Sarai1 1Dept. of Biosci. Bioinf., Kyushu Inst. of Tech. 2NCI, NIH 3Dept. of Biosci., Jamia Millia Islamia Univ. 4Comp. Biol. Res. Cent., AIST 5SAIC-Frederick, Inc., NCI 1P208 Possible contradiction of the consensus mechanism of E. coli sigma-70 and the observed activity of its N- terminus half proteins ○Suganthan Rajan Babu1，Yu mik o Sat o 1，Shoji Yagi1，Gyedu Ampaabeng1，Nobuo Shimamoto1,2 1SOKENDAI 2NIG 1P209 Correlation between rpoD alleles and upper limits of growing temperature. ○Akiko Hatakeyama1，Yumiko Sato 2，Hiroki Nagai3，Hideki Nakayama1,2，Nobuo Shimamoto1,2 1School of Life Sci., SOKENDAI. 2Structural Biology Center, National Institute of Genetics 3Research Institute for Microbial Deseases, Osaka Univ. 1P210 DNA-binding proteins expressed in regenerating newt retina ○Osamu Hisatomi1，Mari Kotoura1，Daisuke Kitano1，Tatsushi Goto1，Akiyuki Hasegawa1，Eiri Ono1，Fumio Tokunaga1,2 1Grad. Sch. of Sci., Osaka Univ. 2CREST, JST 1P211 Analyses of molecular interaction of HLH proteins by FRET assay ○Mari Kotoura1，Tatsushi Goto1，Daisuke Kitano1，Fumio Tokunaga2，Osamu Hisatomi1 1Graduate School of Science, Osaka University 2CREST, Japan Science and Technology Agency 1P212 The relationship between conformation and activity of NifA ○Hisayoshi Tsuihiji1，Yoichi Yamazaki 1，Hironari Kamikubo1，Mikio Kataoka1 1Nara institute of science and technology 1P213 Study on molecular mechanism for tissue specific expression of novel G protein, HrGn found in Halocynthia roretzi. ○Shinya Mori1，Akira Shirato1，Tatsuo Iwasa 1 1Dept.of Materials Science and Engineering, Muroran Institute of Technology 1P214 Analysis of DNA topoisomerase III function in Schizosaccharomyces pombe ○Masashi Inutsuka1，Tomotake Hosokawa1，Takeshi Terasaki 1，Ryo Hanai1 1Department of Life Science, Rikkyo University 1P215 Tetraplex of fission yeast telomeric DNA and unfolding of the tetraplex on the interaction with telomeric DNA binding protein Pot1 Ayako Furukawa1，○Hidetaka Torigoe1 1Tokyo University of Science 1P216 Thermodynamic Characterization of DNA-binding Activity of Human Lon Protease ○Si-Han Chen1，Shih-Hsiung Wu1 1Institute of Biochemistry, Academia Sinica 1P217 Structural and functional characterization of BldD, an essential developmental regulator of Streptomyces coelicolor ○Chang-Jin Lee1，Jeong-Mok Kim1，In-Kwon Kim1，Sa-Ouk Kang1 1Lab. of Biophysics, School of Biological Sciences, and Institute of Microbiology, Seoul National Univ., Seoul 151-742, South Korea 1P218 The C-terminal extension specific to archaeal and eukaryotic DNA ligases modulates the DNA binding activity ○Maiko Tanabe1，Shinichi Kiyonari2，Yoshizumi Ishino2，Hirokazu Nishida1 1Central Research Lab., Hitachi Ltd. 2Dept. of Genetic Resources Technology, Kyushu University 1P219 Structure of the left-handed archaeal RadA filament: a subunit rotation motif controls homologous DNA strand exchange reaction ○Li-Tzu Chen1，Tzu-Ping Ko1，Yuan-Chih Chang2，Kuei-An Lin1，Shih-Chieh Ti1，Chia-Seng Chang2，Ting-Fang Wang1， Andrew H.-J. Wang1 1Institute of Biological Chemistry,Academia Sinica 2Institute of Physics, Academia Sinica 1P220 The DNA-recognition fold of Sso7c4 suggests an ancestral role in the evolution of β-barrel protein family ○Chun-Hua Hsu1，Andrew H.-J. Wang1 1Institute of Biological Chemistry, Academia Sinica

8. Muscle contraction and muscle proteins 1P221 A Functional Region of ELC Responsible for The Formation of 10S Conformation in Smooth Muscle Myosin ○Tsuyoshi Katoh1，Masayuki Takeuchi1，Atsuhiko Ishida1，Takanobu Taniguchi1 1Dept. of Biochem., Asahikawa Med. Col. 1P222 The involvement of Fyn in Ca2+-sensitization of vascular smooth muscle contraction. ○Hozumi Kawamichi1，Fengling Guo1，Hiroko Kishi1，Junying Miao1，Dan Xu1，Katsuko Kajiya1，Sei Kobayashi1 1Dept. Mol. Physiol. & Med. Bioregulation. Yamaguchi Univ. Grad. Sch. of Med. 1P223 Effects of BDM on the transverse stiffness of muscle fibers and myofibrils studied by atomic force microscopy ○Nao Akiyama1，Daisuke Miyashiro1，Yuki Kunioka2，Takenori Yamada1 1Dept. of Physics, Fac. of Science, Tokyo Univ. of Science 2JST, Innov. Plaza Ishikawa 1P224 The diameter and its changes of single skeletal myofibrils examined by atomic force microscopy ○Daisuke Miyashiro1，Hirotaka Fujita1，Atsushi Hamazaki1，Nao Akiyama1，Yuki Kunioka2，Takenori Yamada1 1Dept. of Physics, Fac. of Science, Tokyo Univ. of Science 2JST,Innov.Plaza Ishikawa 1P225 Passive and active tension generation of striated muscles with reference to the domain structure of their connectin/titin. ○Masako Kimura1，Sumiko Kimura2，Shigeru Takemori1 1Department of Physiology, The Jikei University School of Medicine 2Department of Biology, Faculty of Science, Chiba University 1P226 Characterization of Mesoscopic Distortions within an Actin Filament in Vitro ○Kuniyuki Hatori1，Tomoyuki Kikuchi1 1Dept. of Bio-System Eng. 1P227 Length-dependent activation by the ADP-bound myosin in skeletal myofibrils -Implications for the self-regulating mechanism of SPOC- ○Yuta Shimamoto1，Fumiaki Kono1，Madoka Suzuki2，Shin'ichi Ishiwata1 1Department of Physics, School of Science and Engineering, Waseda University 2ASMeW, Waseda University 1P228 Native thin filament of aorta smooth muscle has bound IQGAP1, a scaffolding protein ○Yoh Okamoto1，Wataru Endo 2 1Muroran Institute of Technology, Department of Applied Chemistry, Division of Bioengineering 2Muroran Institute of Technology, Department of Applied Chemistry, Division of Bioengineering 1P229 Distribution of bead displacements in optical-trap single molecule experiments on muscle myosins ○Yuki Kagawa1 1Dept. of Electrical Eng. and Bioscience, Waseda Univ. 1P230 Visualization of the ATP Hydrolysis Intermediates of Myosin II by Electron Cryo-microscopy ○Yoshinori Ohta 1，Nobuhisa Umeki2，Eisaku Katayama3，Shinsaku Maruta2，Takuo Yasunaga1 1Department of Bioscience and Bioinformatics, Faculty of Computer Science and Systems Engineering, Kyushu Institute of Technology 2Department of Bioengineering, Soka University 3Division of Biomolecular Imaging, Institute of Medical Science, The University of Tokyo 1P231 Arrangement of myosin heads in intact and actin-extracted skinned myofibril bundles of striated muscle. ○Shigeru Takemori1，Masako Kimura1，Maki Yamaguchi1，Tetsuo Ohno1，Hiroshi Okuyama2，Hiroko Toyoda2，Naoto Yagi 3，Yuichiro Tanishima1，Taku Yamada1，Keisuke Saitou1 1Dept. Physiol. Jikei Univ. Sch. of Medicine. 2Dept. Physiol. Kawasaki Univ. Sch. of Medicine. 3JASRI (SPring8) 1P232 Flexibility Change in Actin Filaments Induced by Myosin Molecules at a Low ATP Concentration ○Yuusuke Arii1，Kuniyuki Hatori1 1Dept. of Bio-system Eng., Yamagata Univ. 1P233 Spin-spin relaxation of 1H-NMR signals from myofibril suspension of rabbit skeletal muscle. ○Tetsuo Ohno1，Masako Kimura1，Maki Yamaguchi1，Shigeru Takemori1 1Department of Physiology,The Jikei Univ. Sch. of Med 1P234 An establishment and analysis of recombinant wild-type and mutant human cardiac actins. ○Mitsusada Iwasa1,2，Ken-Ichi Sano4，Kayo Maeda1,2，Toshiro Oda 1,2，Yuichiro Maeda 1,2,3 1ERATO Maeda PJT, JST 2RIKEN SPring-8 Center 3Nagoya Univ. 4The Cancer Inst. 1P235 Single molecule measurement of Elasticity of connectin-like 1200K-protein in obliquely striated muscle of a polychaete (Annelida) ○Shinichirou Hatakeyama1，Noboru Izawa1，Yi Zhu2，Henk Granzier2，Sumiko Kimura1 1Department of Biology, Faculty of Science, Chiba University 2Department of VCAPP, Washington State University 1P236 Orientations of Resting Myosin Crossbridges in Skeletal Muscles determined by X-ray Fiber Diffraction ○Kanji Oshima1，Yasunori Takezawa2，Yasunobu Sugimoto2，Katsuzo Wakabayashi2 1The center for Adv. Med. Eng. and Info., Osaka Univ. 2Div. of Biophys. Eng., Sch. of Eng. Sci., Osaka Univ. 1P237 Functional analysis of M47A/E360H mutant actin expressed in Dictyostelium cells ○Hiroaki Kubota1，Togo Shimozawa1，Kei Kubo1，Shin'ichi Ishiwata1 1Department of Physics, School of Science and Engineering, Waseda University 1P238 Molecular basis of troponin-related cardiomyopathy and Ca2+-desensitizing compound ○Fumiaki Yumoto1,2，Naoto Tadano3，Sachio Morimoto3，Qun-Wei Lu3，Koji Nagata2，Iwao Ohtsuki1，Masaru Tanokura2 1Dept. of Physiol. II, Jikei Univ. Sch. of Med. 2Grad. Sch. of Agricul. Life Sci., Univ. of Tokyo 3Grad. Sch. of Med., Kyushu Univ. 1P239 Size distribution of actin oligomers in physiological actin solutions analyzed by photon counting histogram ○Naofumi Terada1，Togo Shimozawa1，Shin'ichi Ishiwata1，Takashi Funatsu 2 1Grad. Sch. of Sci. Eng., Waseda Univ. 2Grad. Sch. of Pharm. Sci., Univ. of Tokyo 1P240 Imaging of single molecule of actin within a single sliding filament ○Yousuke Taguchi1，Ei-ich Imai1，Katsuhiko Shimada2，Hajime Honda1 1Department of BioEngineering, Nagaoka University of Technology, Nagaoka 2School of Design and Architecture, Nagoya City University, Nagoya 1P241 The hydrophobic environment around the moving actin filaments ○Yusuke Shimazaki1，Ei-ichi Imai1，Hajime Honda1 1Dept. of Biology engineering, Nagaoka Univercity of Technology 1P242 Theoretical Study of the Myosin ATPase Mechanism ○Hiroshi Kagawa1，Qi Gao2，Yu suk e Tamu ra 3，Kazuhide Mori4 1Physics Lab., Nippon Med. Sch. 2Tokyo Inst. of Tech. 3SGI Japan, Ltd. 4WCSC 1P243 Switch action of troponin I on the Ca2+-regulated thin filament as revealed by spin-labeling distance measurement using PELDOR Tomoki Aihara1,3，Motoyoshi Nakamura1,3，Shoji Ueki1,4，Masao Miki2，○Toshiaki Arata 1,4 1Dept. Biol. Sci., Grad. Sch. Sci., Osaka University 2Fukui University 3ERATO, JST (Present address) 4CREST, JST 1P244 Conformational states of tropomyosin molecule on muscle thin filament ○Keisuke Ueda1，Chieko Kimura3，Tomoki Aihara3，Shoji Ueki1,4，Masao Miki2,4，Toshiaki Arata 1,4 1Dept. Biol. Sci. Osaka Univ. 2Fukui Univ 3ERATO-JST 4CREST-JST 1P245 Properties of "bio-nanomuscle" at various ATP concentrations analyzed with submillisecond resolution ○Kosuke Watanabe1，Madoka Suzuki2，Shin'ichi Ishiwata2,3 1Grad Sch Pure/Appl Phys, Waseda Univ 2ASMeW, Waseda Univ 3Dept Phys, Sch Sci/Eng, Waseda Univ 1P246 Effect of lattice spacing on SPOC of skeletal myofibrils ○Fumiaki Kono1，Yu ta Shi mamot o 2，Shin'ichi Ishiwata1,2 1Graduate School of Pure and Applied Physics, Waseda University 2Department of Physics, School of Science and Engineering, Waseda University 1P247 Molecular mechanism of stretch activation in insect flight muscle as examined by X-ray diffraction ○Hiroyuki Iwamoto1，Katsuaki Inoue1，Tetsuro Fujisawa2，Naoto Yagi1 1SPring-8, JASRI 2Riken Harima Inst. 1P248 The "active" effects of G680V on the sliding velocity of an actin filament. ○Syun Tsukano1，Ei-ichi Imai1，Hajime Honda1 1Dept. of Bioengineering, Nagaoka Univ. of tech. 1P249 Spatial distortions of actin filaments on the sliding movement in the in vitro motility assay ○Mihoko Kunita1,3，Shigeru Sakurazawa1，Hajime Honda2 1Dept. of Systems Information Science, Future University-Hakodate 2Dept. of BioEngineering, Nagaoka University of Technology 3JSPS Research Fellow 1P250 A FRET model of the Troponin-Tropomyosin complex Chieko Kimura-Sakiyama1，Yutaka Ueno2，Katsuzo Wakabayashi3，○Masao Miki1 1Department of Applied Chemistry and Biotechnology, Fukui University 2National Institute of Advanced Industrial Science and Technology 3Graduate School of Engineering Science, Osaka University 1P251 Function of Two Heads of Rabbit White Skeletal Muscle Myosin in Muscle Contraction ○Akio Inoue1 1Dept. Biol., Grad. Sch. Sci., Osaka Univ. 1P252 Structure Analysis of the Thin Filament Including Troponin by X-ray Diffraction ○Tatsuhito Matsuo1，Yu taka Uen o 2，Yasunori Takezawa1，Katsuzo Wakabayashi1 1Division of Biophysical Engineering, Graduate School of Engineering Science, Osaka University, Tyonaka, Osaka 560-8531 2Neuroscience Research Institute, National Institute of Advanced Science and Technology, Tsukuba, Ibaraki 305-8568 1P253 Modeling of F-actin ○Toshiro Oda 1,2，Yu ich i ro M aed a 2,3 1RIKEN Spring-8 center, RIKEN harima institute, RIKEN 2ERATO Actin Filament Dynamics Project, JST 3 School Biological Sciences, Nagoya Univ

9. Molecular motor (I) 1P254 Conformations and chemical states of F1-ATPase during rotation ○Tomoko Masaike1，Masasuke Yoshida2，Kazuhiro Oiwa3，Takayuki Nishizaka 1 1Dept. of Physics, Gakushuin Univ. 2Chemical Resources Lab., Tokyo Institute of Technology 3National Institute of Information and Communication Technology 1P255 Fiber diffraction study on flagellar axonemes from a wild type and mutant strains of Chlamydomonas ○Shiori Toba1，Hiroyuki Iwamoto2，Tetsuro Fujisawa3，Hitoshi Sakakibara1，Kazuhiro Oiwa1 1Kobe Advanced ICT Research Center, National Institute of Information and Communications Technology 2Research and Utilization Division, Japan Synchrotron Radiation Research Institute, SPring-8 3Biometal Science Laboratory, RIKEN Harima Institute 1P256 Characterization of the Cys-introduced P-ring component FlgI and the stator component MotB in the flagellar motor of Escherichia coli ○Yohei Hizukuri 1，Seiji Kojima1，Ikuro Kawagishi1,2，Michio Homma1 1Div. of Biol. Sci., Grad. Sch. of Sci., Nagoya Univ. 2Inst. for Advanced Research, Nagoya Univ. 1P257 Amino acid residues involved in proton translocation in the MotA/MotB stator complex of the bacterial flagellar motor ○Yong-Suk Che 1,2，Tohru Minamino1,2，Nobunori Kami-ike1,2，Keiichi Namba1,2 1Graduate School of Frontier Biosciences, Osaka University 2Dynamic NanoMachine Project, International Cooperative Research Project, JST 1P258 Fluctuation in sliding movement driven by molecular motors: simulation study ○Yasuhiro Imafuku1，Namiko Mitarai2，Hiizu Nakanishi2，Katsuhisa Tawada1 1Dept. of Biology, Kyushu Univ. 2Dept. of Physics, Kyushu Univ. 1P259 Direct visualization of a chromatin-remodeling protein, Rad54, translocating along single-molecules of double-stranded DNA ○Ichiro Amitani1,2,3，Ronald J. Baskin2，Stephen C. Kowalczykowski1,2,3 1Section of Microbiology, Univ. of California, Davis 2Section of Molecular and Cellular Biology, Univ. of California, Davis 3Center for Genetics and Development, Univ. of California, Davis 1P260 Single Cell Measurement of Intracellular pH of Salmonella using Ratio-metric Fluorescence Measurement of pH- Sensitive GFP, pHluorin ○Nobunori Kami-ike1，Tohru Minamino1,2，Yong-Suk Che 1,2，Keiichi Namba1,2 1Dynamic NanoMachine Project, International Cooperative Research Project, JST 2Graduate School of Frontier Biosciences, Osaka Univ. 1P261 Fluctuation analysis of microtubule sliding over kinesin thick filaments in vitro ○Daigo Hirata1，Toshiki Taba1，Masaki Edamatsu2，Yoko Y. Toyoshima 2，Akira Yamada3，Yasuhiro Imafuku1，Katsuhisa Tawada 1 1Department of Biology, Kyushu University 2Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo 3Biological ICT Group, National Institute of Information and Communications Technology 1P262 Improvement and analysis of ATP synthase crystals ○Yasuo Shirakihara 1，Aya Shiratori1，Satoshi Murakami2，Toshiharu Suzuki3，Masasuke Yoshida3 1National Institute of Genetics 2Osaka University 3Tokyo Institute of technology 1P263 Mutational studies of GTP-binding motif of FlhF in regulation of the number and location of flagella in Vibrio alginolyticus ○Akiko Kusumoto1，Hiroyuki Terashima1，Akari Shinohara1，Toshiharu Yakushi1,2，Seiji Kojima1，Michio Homma1 1Dept. of Biological Science, Nagoya Univ. 2Fac. of Agriculture, Shinshu Univ. 1P264 Control of kinesin-microtubule motility by caged peptides derived from the kinesin C-terminus domain ○Akiko Nomura1,2，Taro Q. P. Uyeda1，Noboru Yumoto1，Yoshiro Tatsu 1 1AIST 2RIKEN 1P265 Localization of Three Chlamydomonas Outer Arm Heavy Chains ○Hiroko Takazaki1，Zhongmei Liu2，Ritsu Kamiya2，Takuo Yasunaga1 1Department of Bioscience and Bioinformatics, Kyushu Institute of Technology 2Department of Biological Sciences, University of Tokyo 1P266 Single molecule FRET observations of the conformational intermediates of kinesin motor protein during processive movement ○Teppei Mori1，Hisashi Tadakuma1，Michiko Nakajima1，Michio Tomishige1 1Department of Applied Physics, The University of Tokyo 1P267 Structural study of sea urchin outer-arm dynein bound to microtubules ○Hironori Ueno1，Azumi Yoshimura2，Takuo Yasunaga3，Chikako Shingyoji2，Keiko Hirose1 1Research Institute for Cell Engineering, National Institute for Advanced Industrial Science and Technology (AIST) 2Department of Biological Sciences, Graduate School of Science, University of Tokyo 3Department of Biochemical Engineering and Science, Kyushu Institute of Technology 1P268 Conformational Changes in a Kinesin Motor Kar3 Catalysed by Interaction with Microtubules ○Keiko Hirose1，Toshihiko Akiba2，Sharyn Endow3，Linda Amos4 1Res. Inst. Cell Engineering, AIST 2Biological Information Res. Center, AIST 3Duke University Medical Center 4MRC Laboratory of Molecular Biology 1P269 Single binding unit of the 'catch' reconstituted in vitro with myosin and twitchin purified from bivalve adductor muscle ○Akira Yamada1，Maki Yoshio1，Kazuhiro Oiwa1 1Biological ICT Group, National Institute of Information and Communications Technology 1P270 Three-dimensional trajectories of the central stalk subunit in stepping rotation of F1-ATPase ○Kaoru Okada1，Kana Mizutani1，Satoko Shibano1，Tomoko Masaike1，Takayuki Nishizaka1,2 1Gakushuin Univ., Fac. Sci., Dept. phys. 2PRESTO, JST, 1P271 Brownian motors driven by transmembrane ion flows ○Takeshi Nakagawa1，Kazuo Sasaki1 1Department of Applied Physics, Tohoku University 1P272 A point mutation in the SH1 helix alters elasticity and thermal stability of myosin II. ○Sosuke Iwai1,2，Daisuke Hanamoto1，Shigeru Chaen1 1Department of Integrated Sciences in Physics and Biology, College of Humanities and Sciences, Nihon University 2National Institute of Advanced Industrial Science and Technology 1P273 Cooperativity causes plasticity in molecular motors ○Seiji Esaki1，Yoshiharu Ishii2，Toshio Yanagida1,2 1Graduate School of Frontier Biosciences, Osaka University 2Formation of soft nano-machines, CREST 1P274 Structural dynamics of acto-myosin V revealed by high-speed AFM ○Noriyuki Kodera1，Daisuke Yamamoto1,2，Toshio Ando1,2 1Dept. of Physics, Kanazawa Univ. 2CREST/JST 1P275 A dynein/microtubule system that can partially mimic the motile properties of flagellar axonemes ○Susumu Aoyama1，Ritsu Kamiya1,2 1Dept. of Biological Sciences, Univ. of Tokyo 2CREST, JST 1P276 Analysis of AFM images of Protein in Light of its Atomic Model ○Mitsuru Sakashita1，Noriyuki Kodera1，Hidemi Nagao2，Atsuko Iwane Hikikoshi3，Toshio Yanagida3，Toshio Ando1,4 1Dept. of Physics, Kanazawa Univ. 2Dept. of Computational Science, Kanazawa Univ. 3Graduate School of Frontier Biosciences, Osaka Univ. 4CREST/JST 1P277 Calcium Regulation of Myosin V ○Shin'ichiro Tanaka1，Naoki Nagura1，Yu ta Sai t o 1，Toshio Ando1,2 1Dept.of physics,Kanazawa Univ 2CREST/JST 1P278 Myosin V walks by lever action and Brownian motion ○Katsuyuki Shiroguchi1，Kazuhiko Kinosita, Jr.1 1Dept. of Phys., Faculty of Sci. and Eng., Waseda Univ. 1P279 Flexible and rigid regions in the stalk domain of ncd motor protein ○Tsukasa Makino1，Hisayuki Morii2，Takashi Shimizu 2，Fumio Arisaka3，Yu suk e Kat o 1，Koji Nagata1，Masaru Tanokura1 1Dept. Appl. Biol. Chem., Grad. Sch. Agric. Life Sci., Univ. Tokyo 2Inst. Biol. Funct. Resources, A.I.S.T. 3Dept. Biomol. Processing, Grad. Sch. Biosci. & Biotech.,Tokyo Inst. Tech. 1P280 Molecular dynamics simulation of Fo rotary motor: rotational diffusion and proton pathway ○Hisatsugu Yamasaki1，Mitsunori Takano1 1Dept. of Physics, Waseda Univ. 1P281 Torque-speed relationship of Na+-driven chimeric flagellar motor in Escherichia coli. ○Yuichi Inoue 1，Yoshiyuki Sowa2，Michio Homma3，Akihiko Ishijima1 1IMRAM, Tohoku Univ. 2Dept. of Physics, Univ. of Oxford 3Division of Biological Science, Nagoya Univ 1P282 Attempt of functional reconstitution of stator of the bacterial flagellar motor ○Hiroyuki Terashima1，Seiji Kojima1，Michio Homma1 1Division of Biological Science, Graduate School of Science, Nagoya univ. 1P283 Identification of two novel inner-arm dynein heavy chains in Chlamydomonas flagella ○Toshiki Yagi1，Ritsu Kamiya1,2 1Department of Biological Science, Graduate School of Sciences, Univertisy of Tokyo 2CREST, Japan Science and Technology Agency 1P284 The behavior of F1 motor in the presense of the uniform external torque applied with electrorotation ○Takahiro Nakayama-Watanabe1，Eiro Muneyuki2，Seishi Kudo3，Shigeru Sugiyama4，Masasuke Yoshida1 1Chemical Resources Lab., Tokyo Tech. 2Dept. of Physics, Chuo Univ. 3Dept. of Engineering, Toin Univ. of Yokohama 4NFRI 1P285 The mode of interaction between myosin V and actin filament ○Jun Kozuka1,2，Yoshiharu Ishii2，Toshio Yanagida1,2 1Soft Biosystem Group, Laboratories for Nanobiology, Graduate School of Frontier Biosciences, University of Osaka 2Formation of Soft Nanomachines, Core Research for Evolution Science and Technology, Japan Science and Technology Agency 1P286 Analysis of unconventional myosins by spectroscopic electron cryo-microscopy. ○Shingo Fujiwara1，Yousuke Kano1，Osamu Sato2，Kazuaki Honma2，Reiko Ikebe2，Mitsuo Ikebe2，Takuo Yasunaga1 1Department of Bioscience and Bioinfomatics, Computer Science and System Engineering, Kyushu Institute of Technology Graduate School 2Univ. of Massachusetts Medical School 1P287 Structural analysis of the flagella in the soybean symbiont Azospirillum brasilence ○Masaomi Kanbe1,3，Tatsuaki Ebisawa1，Bonnie Stephens2，Gladys Alexander2，Shin-Ichi Aizawa1,3 1Department of Life Sciences, Prefectural University of Hiroshima 2Georgia State University 3CREST, Japan Science & Technology Agency(JST) 1P288 One-dimensional diffusion of charged nanoparticles along microtubules ○Itsushi Minoura1，Etsuko Muto1 1RIKEN BSI 1P289 Single-molecule observation of the in vitro movement of flagellar outer dynein arm complex ○Akane Okawa1，Toshiki Yagi1，Tomonobu Watanabe2，Hideo Higuchi2，Ritsu Kamiya1 1Dept. of Biological Science, Graduate School of Science, University of Tokyo 2Biomedical engineering Research Organizaition, Tohoku University 1P290 Molecular dynamics simulation of biased motion of myosin head along actin filament ○Tomoki P. Terada 1，Masaki Sasai1 1Dept. of Computational Science and Technology, Grad. Sch. of Engineering, Nagoya Univ. 1P291 Crystal structure of MotY ; an essential component of the torque generator of Na+-driven flagellum in Vibrio alginolyticus ○Akari Shinohara1，Katsumi Imada2,3，Mayuko Sakuma4，Toshiharu Yakushi5，Seiji Kojima4，Michio Homma1,4 1CREST, JST 2Graduate School of Frontier Biosci., Osaka Univ. 3ICORP, JST 4Div. of Biol. Sci., Graduate School of Sci., Nagoya Univ. 5Dept. of Biosci. and Biotech. Shinsyu Univ. 1P292 A possible mechanism for determining the directionality of myosin molecular motors ○Tadashi Masuda 1 1School of Biomedical Science, Tokyo Medical and Dental University 1P293 The construction of measurement system for simultaneous observation of myosin V ○Tomotaka Komori1，So Nishikawa2，Takayuki Ariga1，Atsuko H. Iwane1，Hisashi Yamakawa3，Osamu Ohara3，Toshio Yanagida1,2 1Graduate School of Frontier Biosciences, Osaka Univ. 2JST, CREST 3Kazusa DNA Research Institute 1P294 ADP affinity of myosin VI is modulated by load and depends on loading direction ○Sergey V. Mikhailenko1，Yusuke Oguchi1，Adrian O. Olivares2，Enrique M. De La Cruz2，Shin'ichi Ishiwata1 1Dept. of Physics, Waseda Univ. 2MB&B Dept., Yale Univ. 1P295 Characterization of MyosinVI isolated from sea urchin eggs ○Souhei Sakata1，Jiro Usukura2，Issei Mabuchi1 1Div.Biol., Grad.Sch.Arts & Sci., Univ.Tokyo 2Center Res., Adv.Sci.&Tech., Nagoya Univ. 1P296 Two-dimensional observation of stepping motion of single kinesin molecules along microtubule ○Yuya Miyazono1，Keishi Kikuchi1，Hisashi Tadakuma1，Michio Tomishige1 1Dept. of Applied Physics, Tokyo Univ. 1P297 Direct observation of T7 RNA polymerase rotation during transcription. ○Yuko Kato 1，Hiroaki Yokota1，Masahito Hayashi1，Kayoko Terada1,2，Yasuhiro Sasuga1,2，Yoshie Harada 1,2 1The Tokyo Metropol. Inst. of Med. Sci. 2CREST, JST 1P298 Thermodynamics of stepsize and signal production in myosin molecular motor system. ○Hiroto Tanaka1,2，Toshio Yanagida1,2 1Formation of soft nano-machines, CREST, JST 2Grad. Sch. of Frontier Biosciences, Osaka Univ. 1P299 The micro needle study of the relationship between myosin and a single actin filament ○Masatoshi Morimatsu1，So Nishikawa2，Yoshikazu Tsukasaki1，Takuya Okada2，Atsuko H Iwane1，Toshio Yanagida1 1Graduate school of Frontier biosciences, Osaka Univ 2Softnano, CREST, JST 1P300 Two modes of microtubule-sliding driven by cytoplasmic dynein ○Tomohiro Shima 1，Takahide Kon1，Reiko Okura1，Kazuo Sutoh1 1Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo 1P301 ε Subunit Stops Rotation of Thermophilic F1-ATPase;Single Molecular Analysis of the inhibition by the ε subunit. ○Takamitsu Haruyama1，Yoko Hirono-Hara2，Hiroyuki Noji3，Yasuyuki Kato-Yamada1 1Department of Life Science, College of Science, Rikkyo (St. Paul's) University 2Institute of Industrial Science, The University of Tokyo 3The Institute of Scientific and Industrial Research, Osaka University 1P302 A refined Brownian motor model for the single-headed kinesin KIF1A ○Ryo Kanada1，Kazuo Sasaki2 1Cybermedia Center, Osaka Univ 2Dept. of Appl. Phys., Tohoku Univ 1P303 Kinetic mechanism of the Fastest Motor Protein, Chara Myosin ○Kohji Ito1，Mitsuo Ikebe2，Taku Kashiyama 1，Toshifumi Mogami3，Takahide Kon3，Keiichi Yamamoto1 1Dept. of Biology, Chiba Univ. 2Dept. of Physiology, Univ. of Massachusetts Medical School 3Dept. of Life Science, Tokyo Univ.

10. Cytoskeletons 1P304 Crystal structure of Formin Homology 2 domain of human DAAM1 ○Masami Yamashita1，Tomohito Higashi 3，Yusuke Sato1，Ryutaro Shirakawa3，Toru Kita 3，Hisanori Horiuchi3，Shuya Fukai1,2，Osamu Nureki1 1Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology 2Center for Biological Resources and Informatics, Tokyo Institute of Technology 3Department of Cardiovascular Medicine, Graduate School of Medicine, Kyoto University 1P305 The binding mechanism of capping protein (CP) to barbed and of actin filament ○Shuichi Takeda1，Akihiro Narita1，Hiroshi Morii1，Yuichiro Maeda 1,2 1ERATO, JST 2Grad. Sch. of Sciences, Nagoya Univ. 1P306 Drebrin-A, a spine specific actin-binding protein in neurons, inhibits the activities of ADF/cofilin, caldesmon, and myosin-V. ○Ryoki Ishikawa1，Kaoru Katoh2，Ayumi Takahashi1，Ce Xie1，Koushi Oseki3，Michitoshi Watanabe3，Michihiro Igarashi3， Akio Nakamura1，Kazuhiro Kohama1 1Detp. Mol. Cell. Pharmacol., Gunma Univ. Grad. Sch. Med. 2Neurosci. Res. Inst., NAIST 3Div. Mol. Cell. Biol., Niigata Univ. Grad. Sch. Med. Dent. Sci. 1P307 Cell polarization accompanies stress fiber formation in spreading fibroblasts ○Yosuke Senju1，Hidetake Miyata1 1Dept. of Physics, Tohoku Univ. 1P308 Imaging of living tissue culture cell by AFM and a deforming effect under high pressure environment ○Yasuaki Yamamoto 1，Hideyuki Yoshimura2，Tetsuya Miwa 3 1Dept. of physics, Meiji Univ 2Dept. of physics, Meiji Univ. 3Dept. of XBR, JAMSTEC 1P309 Elasticity of Solutions of Actin Filaments with Polymorphous Assembly Structures ○Tadanao Ito1,2，Masahito Yamazaki3 1Research Center for Low Temperatures and Materials Sciences, Kyoto University 2Graduate School of Science, Kyoto University 3Department of Physics, Faculty of Science, Shizuoka University 1P310 Mechanical properties of a single microtubule measured by optical tweezers; The effect of kinesin binding ○Yuji Ozaki 1，Togo Shimozawa1，Masako Ohtaki2，Shin'ichi Ishiwata1 1Graduate School of Pure and Applied Physics, Waseda University 2Visiting Lecturer, School of Science and Engineering, Waseda University 1P311 Measurements of microtubule-dependent forces acting on mitotic spindle ○Takeshi Itabashi1，Yuta Shimamoto1，Jedidiah Gaetz2，Jenny Z. Liu2，Tarun M. Kapoor2，Shin'ichi Ishiwata1 1Dept. Phys., Sch. Sci./Eng., Waseda Univ. 2Lab. Chem./Cell Biol., The Rockefeller Univ. 1P312 Microviscoelasticity mapping of the environment around the mitotic spindle extracted from Xenopus egg ○Yuta Abe 1，Takeshi Itabashi1，Yu ta Sh i mamot o 1，Shizuka Sato1，Togo Shimozawa1，Tarun M. Kapoor2，Shin'ichi Ishiwata1 1Dept. Phys., Sci./Eng., Waseda Univ. 2Lab. Chem./Cell Biol., The Rockefeller Univ. 1P313 Fluorescence phalloidin binding to I-filaments of skeletal muscle: Both ends of an I-filament are different ○Natsumi Tsujimoto1，Masaaki Kuroda1 1Dept. of Biological Science, Shimane Univ. 1P314 Digitally controlled feedback system developed for the optical tweezers ○Shouhei TSUKAHARA1，Togo SHIMOZAWA2，Shin'ichi ISHIWATA2 1Graduate school School of Integrative Bioscience and Biomedical Engineering, Waseda University 2Dept. of Physics, School of science Science and engineeringEngineering, Waseda University 1P315 Modulation of the fluorescence intensity of a single actin filament by the tensile force. ○Togo SHIMOZAWA1，Shin'ichi ISHIWATA1 1Dept. of Physics, Waseda Univ. 1P316 Direct observation of cofilin-actin interaction by fluorescence microscopy ○Chikanori Ohno1，Togo Shimozawa1，Naruki Sato2，Shin'ichi Ishiwata1 1Dept. of Physics, Waseda University 2Department of Biology, Faculty of Science, Chiba University 1P317 The analysis of the binding between F-actin and Microtubule-associated protein 4 isoforms. ○Hirokazu Nakatake1，Kazuyuki Matsushima2，Susumu Kotani2，Kiyotaka Tokuraku1 1Dept. of Chem. Sci. and Eng., Miyakonojo Nat. Col. of Tech. 2Dept. of Biological Sci., Faculty of Sci., Kanagawa Univ. 1P318 Mechanical tension in actin filaments prevents the filaments from disassembly by cofilin. ○Kimihide Hayakawa1，Hitoshi Tatsumi2，Masahiro Sokabe1,2,3 1Cell Mechanosensing Project, SORST, JST 2Dept. of Physiology, Nagoya University Graduate School of Medicine 3Dept. of Molecular Physiology, NIPS, NINS 1P319 Differences in the regulation of microtubule stability by microtubule-associated protein 4 isoforms ○Hatsune Moriyama1，Shigeaki Miyamoto1，Susumu kotani2，Kiyotaka Tokuraku3 1Dept. of Biosience and Bioinformatics, Kyushu Inst. of Tech. 2Dept. of Biological Sci., Faculty of Sci., Kanagawa Univ. 3Dept. of Chem. Sci. and Eng., Miyakonojo Nat. Col. of Tech. 1P320 Gas7 increases of microtubule polymerization coupled with the enhancement of the polymerization-intermediate sheet ○Tomohiko J. Itoh1，Hirotada Akiyama2，Takafumi Uchida2 1Div. Biol. Sci., Nagoya Univ. 2Gra. Sch. Agric. Sci., Lab. Enzymol., Tohoku Univ., 1P321 Ferritin forms dynamic oligomers to associate with microtubules in vivo: Implication for the role of microtubules in iron metabolism. ○Hiroyuki Nakagawa1,2，Mohammad_R Hasan2，Sayaka Koikawa2，Susumu Kotani3，Shigeaki Miyamoto2 1Faculy of Scince, Fukuoka University 2Department of Bioscience and Bioinformatics, Kyushu Institute of Technology 3Faculty of Science, Kanagawa University 1P322 A microtubule-associated protein 4 specific five-repeat isoform inhibits kinesin-driven microtubule gliding ○Kiyotaka Tokuraku1，Taro Noguchi1,2，Makiko Nishie1，Kazuyuki Matsushima3，Susumu Kotani3 1Miyakonojo National College of Technology 2National Institute for Advanced Industrial Science and Technology 3Kanagawa University 1P323 Molecular basis of microtubule disassembly mechanism regulated by katanin p60. ○Naoko Iwaya1,2，Satoru Unzai2，Kentaro Tomii3，Natsuko Goda2，Hidehito Tochio1，Masahiro Shirakawa1，Hidekazu Hiroaki2 1Grad. Sch. of Eng., Kyoto Univ. 2Int. Grad. Sch. of Arts and Sci., Yokohama City Univ. 3CBRC, AIST 1P324 Structural state transitions of G-actin studied by molecular dynamics simulation ○Taira Hayashida 1，Mitsunori Takano2 1Dept. of Bioscience and Biomedical Engineering, Science and Engineering, Waseda Univ. 2Dept. of Physics, Science and Engineering, Waseda Univ.

11. Morphogenesis and cell adhesion 1P325 The effect of active peptide mobility and conformation on cell morphology ○Teruaki YOSHIDA1，Takuya NODZUMI2，Daisuke AKIEDA2，Satoshi SIMAMOTO2，Tomoyasu AIZAWA1,2，Keiichi KAWANO2，Makoto DEMURA1 1Grad. Sch of LifeSci., Hokkaodo Univ. 2Grad. Sch of Sci., Hokkaodo Univ. 1P326 Structural analysis of uro-adherence factor A, UafA, from Staphylococcus saprophyticus ○Eriko Matsuoka1，Yoshikazu Tanaka1，Makoto Kuroda2，Min Yao1，Nobuhisa Watanabe1，Toshiko Ohta 2，Isao Tanaka1 1Faculty of Advanced Life Science, Hokkaido Univ. 2Dept. of Microbiology, Grad. School of Comprehensive Human Science, Univ. of Tsukuba 1P327 Mechanical force-dependent zyxin accumulation leads to actin polymerization at focal adhesions. ○Hiroaki Hirata1,2，Hitoshi Tatsumi3，Masahiro Sokabe1,2,3 1ICORP/SORST, Cell Mechanosensing Project, Japan Science and Technology Agency 2Dept. of Molecular Physiology, National Institute for Physiological Sciences 3Dept. of Physiology, Graduate School of Medicine, Nagoya Univ. 1P328 Molecular mechanism of stretch-induced deadhesion of integrin in endothelial cells: involvement of clathrin-dependent endocytosis. ○Daisuke Kiyoshima1，Keisuke Kawakami2，Kimihide Hayakawa3，Hitoshi Tatsumi1，Masahiro Sokabe1,3,4 1Dept. Physiol., Grad. Sch. Med., Univ. Nagoya, Nagoya, Japan 2Dept. Phys. Ther., Sch. Health Sci., Univ. Nagoya, Nagoya, Japan 3SORST Cell-Mechanosensing Project, JST, Nagoya, Japan 4Dept. of Molecular Physiology, NIPS. NINS, Okazaki, Japan 1P329 Effects of cell-cell contact on anoikis induction ○Yumiko Emoto 1 1Dept. of Biology, Kyushu Univ 1P330 Spontaneous formation of cell clusters in a primary culture of cardiac myocytes ○Akihiro Isomura1，Takahiro Harada2 1Department of Physics Graduate School of Science Kyoto University 2Department of Human and Artificial Intelligent Systems, University of Fukui 1P331 The energy metabolism during formation of the multicellular structure ○Tatsufumi Nomura1，Chiaki Hidai2，Seiichiro Nakabayashi1 1Dept. of Chem., Saitama Univ. 2Dept. of Physiol., Nihon Univ. Sch. Med. 1P332 Polarized epithelial morphogenesis by cellular active rearrangement - computer simulation ○Hisao Honda1，Tatsuzo Nagai2，Masaharu Tanemura3 1Hyogo Univ. 2Kyushu Kyoritsu Univ. 3Inst. of Statistical Math

12. Membrane dynamics 1P333 Theoretical Study of Phospholipid Liposome ○Toshihiko Ogihara1，Hiroshi Kagawa2，Yu ji Takamot o 3，Qi Gao4，Yusuke Tamura5，Chikaomi Ohe6 1Dept. of Gen. Sci., Azabu Univ. 2Physics Lab., Nippon Med. Sch. 3Sapporo Holdings Ltd. 4Tokyo Inst. of Tech. 5SGI Japan, Ltd. 6Dept. of Chem., Waseda Univ. 1P334 Investigation on vesicle fission of biomembrane by the single GUV method [3]. ○Yasuyuki Inaoka1，Masahito Yamazaki2 1Dept. of Phys., Fac., Sci., Shizuoka Univ. 2Int. Biosci. Sec., Grad. Sch. of Sci. and Eng., Shizuoka Univ. 1P335 Low pH-induced phase transition from Lα to cubic phases of biomembranes ○Yoshihide Okamoto 1，Masahito Yamazaki2 1Dept.Phys., Fac.Sci., Shizuoka Univ. 2Int.Biosci.Sec., Grad.Sch of Sci. and Eng., Shizuoka Univ. 1P336 Specific interaction of the fluorescent probe derived from acylated 3β-cholesteryl amine with sphingolipid ○Yoshikatsu Ogawa1，Mari Gotoh1，Tomoko Okada1，Osamu Niwa1，Mutsuo Tanaka1 1National Institute of Advanced Industorial Science and Technology (AIST) 1P337 Physical properties of liposomes observed by various methods and its relation to functions of membranes ○Li WU1，Kaoruko Uramoto1，Tetsuhiko Ohba1，Kazuo Ohki1 1Department of Physics, Graduate School of Science, Tohoku University 1P338 Comparative study on the stabilizing effect of cholesterol on lamellar bilayers with FCS and PFG-NMR ○Thorsten Pieper1，Svetlana Markova2，Masataka Kinjo1，Dieter Suter2 1Lab, of Supramolecular Biophysics, Hokkaido Univ., Sapporo 2Experimental Physics III, University of Dortmund 1P339 Biomechanical Study of the Interaction between Lipid Bilayer and Membrane Proteins ○Taichi Tamanaka 1，Masao Oka1，Megumi Yasuda1，Rehana Afrin1，Atsushi Ikai1 1Dept. of Bioscience and Biotechnology, Tokyo Institute of Technology 1P340 Functional Gap Junctions between Liposomes and Cells. ○Shin-ichiro M. Nomura1，Wakiko Asayama1，Kazunari Akiyoshi1 1Tokyo Medical & Dental Univ., Institute of Biomaterials & Bioengineering, 21stCOE 1P341 Single Giant Unilamellar Vesicle Method Reveals Interaction of (-)-Epigallocatechin Gallate, Tea Catechin, with Lipid Membranes Yukihiro Tamba1，○Shinya Ohba2，Hiroe Yoshioka3，Masahito Yamazaki4 1IJRC., Shizuoka Univ. 2Dept. Phys., Fac. Sci., Shizuoka Univ. 3RRC., Fac.Sci, Shizuoka Univ. 4Int. Biosci. Sec., Grad. Sch. of Sci. and Eng., Shizuoka Univ. 1P342 Effects of fusogenic peptides on substrate supported planar lipid bilayers. ○Takehiko Inaba1，Yoshiro Tatsu 1，Kenichi Morigaki1 1AIST 1P343 Several factors affecting the pore formation of Antimicrobial Peptide, Magainin 2 ○Yukihiro Tamba 1，Takuya Yoshitani2，Junji Mano2，Masahito Yamazaki3 1IJRC, Shizuoka Univ. 2Dept. of Phys., Fac. of Sci., Shizuoka Univ. 3Int. Biosci. Sec., Grad. Sch. of Sci. and Eng., Shizuoka Univ. 1P344 Membrane perturbation by antimicrobial peptides from amphibian and arachnid species ○Martin P. Boland1，Tara L. Pukala 2，John D. Gehman1，Lucia Kuhn-Nentwig3，John H. Bowie2，Frances Separovic1 1School of Chemistry, University of Melbourne, VIC 3010 2Department of Chemistry, University of Adelaide, SA 5005 3Zoological Institute, University of Bern, Baltzerstrasse 6, CH-3012, Bern 1P345 Conformational analysis of α-MSH bound to phospholipids bilayers using solid state NMR ○Eisuke Takeda1，Katsuyuki Nishimura2，Atsushi Kira3，Akira Naito1 1Grad. Sch. Eng., Yokohama Natl. Univ. 2Inst. Mol. Sci. 3ULVAC 1P346 Conformation and interaction of β-endorphin with a model membrane consisting of unsaturated lipid bilayers as studied by solid-state NMR ○Emi Taniguchi1，Katsuyuki Nishimura2，Akira Naito1 1Grad. Sch. Eng., Yokohama Natl. Univ. 2Inst. Mol. Sci. 1P347 Interaction of ACTH with cholesterol containing lipid bilayers as studied by solid state NMR ○Takahiro Noguchi1，Akihiro Myoga1，Katsuyuki Nishimura2，Akira Naito1 1Grad.Sch.Eng.,Yokohama Natl.Univ. 2Inst.Mol.Sci 1P348 Dynamic structures of somatostatin-14 (SS-14) and octreotide bound to phospholipid bilayers on studied by solid state NMR ○Yuhei Okada 1，Katsuyuki Nishimura2，Junya Sasaki1，Atsushi Kira3，Akira Naito1 1Grad. Sch. Eng. Yokohama Nati, Univ. 2Inst. Mol. Sci. 3ULVAC 1P349 pH Effect on the Interaction between Cubic Phaese-Membranes and De Novo Designed Peptides Partitioned into their Membrane Interface ○Shah _ Md. Masum1,2，Masahito Yamazaki2 1Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhaka 2Int. BioSci. Sec., Grad. Sch. of Sci. and Eng., Shizuoka Univ. 1P350 Comparative Study on Selective Interaction of LfcinB-25 and LfcinB-6 with Acidic Phospholipid Bilayers and its Antimicrobial Activity ○Masako Umeyama1，Atsushi Kira2，Akira Naito1 1Grad. Sch. of Engineering, Yokohama Natl. Univ. 2ULVAC, Inc.

13. Membrane transport 1P351 Membrane transport assay system for transporter proteins using artificial lipid bilayers ○Hiroaki Suzuki1，Kazuhito Tabata2，Hiroyuki Noji2，Shoji Takeuchi1 1The University of Tokyo, Institute of Industrial Science 2Osaka University, The Institute of Scientific and Industrial Research 1P352 Cell characterization method based on transport ability of fluorescent substrate ○Yu Anzai 1，Hiroyuki Moriguchi2，Kenji Yasuda1,3 1Graduate School of Arts and Sciences, University of Tokyo 2Graduate School of Frontier Sciences, University of Tokyo 3Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University 1P353 Ion transport across a bilayer lipid membrane facilitated by gramicidin A-Effect of counter anions on the cation transport- ○Osamu Shirai1，Shunsuke Ozaki1，Jun Ohnishi1，Yumi Yoshida 2，Sorin Kihara2，Kenji Kano1 1Graduate School of Agriculture, Kyoto Univ. 2Dept. of Chemistry, Kyoto Inst. of Technol. 1P354 Electrochemical elucidation on ion transport across a bilayer lipid membrane -The role of hydrophobic ions as carrier of the counter ion- ○Shunsuke Ozaki1，Osamu Shirai1，Sorin Kihara2，Kenji Kano1 1Graduate School of Agriculture, Kyoto University 2Department of Chemistry, Kyoto Institute of Technology 1P355 ATP cannot be an energy source for the P-type ATPase-mediated cation transport in the Post-Albers scheme ○Yoshihiro Fukushima1，Makoto Ushimaru1 1Dept. of Chemistry, Kyorin Univ. School of Med. 1P356 Crystallization and X-ray diffraction of SecYE translocon from Thermus thermophilus with a Fab fragment ○Tomoya Tsukazaki 1，Hiroyuki Mori2，Ryuichiro Ishitani1，Shuya Fukai1，Anna Perederina3，Dmitry G. Vassylyev3， Koreaki Ito2,4，Osamu Nureki1,5 1Grad. Sch. of Biosci. and Biotechnol., Tokyo Inst. of Tech. 2Inst. for Virus Res., Kyoto Univ. 3Dep. of Biochem. and Mol. Gen., Univ. of Alabama 4CREST, JST 5PRESTO, JST 1P357 Crystal structure of yeast Sec2p, a guanine nucleotide exchange factor for yeast Rab GTPase, Sec4p. ○Yusuke Sato 1，Ryutaro Shirakawa3，Hisanori Horiuchi3，Naoshi Dohmae4，Shuya Fukai1,2，Osamu Nureki1 1Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology 2Center for Biological Resources and informatics, Tokyo Institute of Technology 3Department of Cardiovascular Medicine, Graduate School of Medicine, Kyoto University 4Biomolecular Characterization, RIKEN 1P358 Structural analysis of the Mammalian Exocyst Complex ○Shuya Fukai1,2，Yusuke Maruyama 3，Ryutaro Shirakawa4，Hisanori Horiuchi4，Chikara Sato3，Osamu Nureki2 1Center for Biological Resources and Informatics, Tokyo Tech. 2Graduate School of Bioscience and Biotechnology, Tokyo Tech. 3Neuroscience Research Institute, AIST 4Graduate School of Medicine, Kyoto Univ. 1P359 Involvement of Rabs in the intracellular trafficking of secretory granules in mast cells ○Noriko Nakagawa1，Mamoru Nakanishi2，Naohide HIrashima1 1Graduate School of Pharmaceutical Sciences, Nagoya City University 2School of Pharmacy, Aichi Gakuin university 1P360 Imaging of COPII vesicle formation and budding from artificial membrane. ○Kazuhito Tabata1，Ken Sato2，Toru Ide3，Hiroyuki Noji1 1ISIR, Osaka Univ. 2Mole. memb. biol., RIKEN 3Front. Biosci., Osaka Univ.

14. Ion channels and receptors 1P361 MSC1 is a mechanosensitive channel in the intracellular membrane of Chlamydomonas. ○Yoshitaka Nakayama1，Kenta Fujiu2，Masahiro Sokabe2,3,4，Kenjiro Yoshimura1,2,5 1 Struc. Biosci., Grad. Sch. Life Environ. Sci., Univ. Tsukuba, Tsukuba. 2Cell-Mechanosensing, ICORP/SORST, JST, Nagoya. 3 Dept. Physiol., Nagoya Univ. Grad. Sch.Med., Nagoya. 4 Dept. Mol. Physiol., Natl. Inst. Physiol. Sci., Okazaki. 5Okazaki. Inst. Integ. Biosci., Natl. Inst. Natural Sci., Okazaki. 1P362 pH-dependent gating of E. coli MscL is controlled by a cluster of charged residues ○Anna Kloda1，Boris Martinac1，Alexandre Ghazi2 1School of Biomedical Sciences, University of Queensland, Brisbane 2Laboratoire des Biomembranes, Universite Paris-Sud, Orsay Cedex 1P363 An attempt to visualize conformational changes during channel gating in the bacterial mechanosensitive channel, MscL ○Hiroaki Machiyama1，Hitoshi Tatsumi1，Masahiro Sokabe1,2,3 1Department of Physiology, Nagoya University Graduate School of Medicine 2ICORP/SORST Cell Mechanosensing Project, Japan Science and Technology Agency 3Department of Molecular Physiology, National Institute for Physiological Sciences 1P364 The Role of TRP Channels in Mechanotransduction of HUVEC ○Yuki Katanosaka1，Tomohiko Suemori1，Keiji Naruse1 1Dept. of Cardiovasc. Physiol., Okayama Univ. 1P365 Immunofluorescence microscopy of TRPC proteins and Gq/11 in the plasma membrane of bovine ciliary muscle cells ○Motoi Miyazu1，Fuminori Yasui2，Hiroshi Ohinata1，Yoshiko Takai3，Akira Takai1 1Dept. Physiol., Asahikawa Med. Coll. 2Dept. Ophthalmol., Asahikawa Med. Coll. 3Dept. Ophthalmol., Sch., of Med. Nagoya Univ. 1P366 Single Molecule Imaging of Stretch-Activated BKca Channels on the Plasma Membrane of Live Cells: Immobilization on Focal Contacts ○Takeshi Kobayashi1，Yoshie Takeda 1，Keiji Naruse2，Masahiro Sokabe1,3 1Dep. Physiol., Nagoya Univ. Grad. Sch. Med. 2Dep. Cardiovasc. Physiol., Okayama Univ. Grad. Sch. Med., Dent. Pharmaceu. Sci. 3ICORP/SORST, Cell Mechanosensing, JST 1P367 Whole-cell patch-clamp analysis of large conductance, stretch-activated, Ca2+-activated K+ channel in chick cultured ventricular myocytes ○Masahiro Kishio1，Keiji Naruse1 1Dept of Cardiovasc. Physiol., Okayama Univ. 1P368 Changes in surface exposing sites of the KcsA potassium channel upon active gating: Effect of a channel blocker, tetrabutylammonium (TBA) ○Masayuki Iwamoto1,3，Hirofumi Shimizu1,3，Fumiko Inoue1,3，Takashi Konno1,3，Yu ji C. Sasak i 2,3，Shigetoshi Oiki1,3 1Dept. Mol. Physiol. Biophys., Fac. Med. Sci., Univ. Fukui 2JASRI, SPring-8 3JST/CREST SASAKI-Team 1P369 Single channel properties of lysenin measured in the artificial lipid bilayer ○Takaaki Aoki 1，Yuko Takeuchi1，Toru Ide1,2 1Graduate School of Frontier Biosciences, Osaka University 2Molecular-Informational Life Science Research Group, RIKEN 1P370 Lysenin forms a voltage dependent channel in artificial lipid bilayer membranes ○Toru Ide1,2，Yuko Takeuchi1，Takaaki Aoki1 1Graduate School of Frontier Biosciences, Osaka University 2Molecular-Informational Life Science Research Group, RIKEN 1P371 The role of GABA receptor in odor information processing in Limax varentianus ○Suguru Kobayashi1，Ryota Matsuo1，Etsuro Ito1 1Facl. Pharmaceut. Sci., Tokushima Bunri Univ. 1P372 Utilization of synthetic fluorescent agonist of nAChR for simultaneous optical and electrical single molecule measurements ○Yuko Takeuchi1，Takaaki Aoki 1，Makoto Ihara2，Yoshinaga Yoshimura 3，Kenzo Fujimoto3，Kazuhiko Matsuda2，Toru Ide1 1Graduate School of Frontier Bioscience, Osaka Uni. 2Graduate School of Agriculture, Kinki Uni. 3The School of Materials Science, Japan Advanced Institute of Science and Technology 1P373 Crystal structure reveals the mechanism for highly specific interaction between CHP and NHE family and its role in pH regulation ○Youssef Ben Ammar1,3，Soichi Takeda2，Takashi Hisamitsu1，Hidezo Mori2，Shigeo Wakabayashi1 1Dept. of Mol. Physiol., National Cardiovascular Center Research Institute 2Dept. of Card. Physiol., National Cardiovascular Center Research Institute 3Japan Society for the Promotion of Science, JSPS 1P374 Normal Mode Analysis of a Small Membrane Polypeptide ○Takaharu Mori1，Hironori Kokubo2，Hirofumi Shimizu3，Masayuki Iwamoto3，Shigetoshi Oiki3，Yuko Okamoto1 1Dept. of Physics, Nagoya Univ. 2Dept. of Chemistry, Univ. of Houston 3Dept. of Mol. Physiol. Biophys., Univ. of Fukui 1P375 Single-molecular gating dynamics of KcsA potassium channel measured by diffracted X-ray tracking ○Hirofumi Shimizu1,2，Masayuki Iwamoto1,2，Fumiko Inoue1,2，Takashi Konno1,2，Yu ji _C. Sasak i 1,3，Shigetoshi Oiki1,2 1JST/CREST SASAKI-Team 2Department of Molecular Physiology and Biophysics, Faculty of the Medical Sciences, University of Fukui 3JST/JASRI 1P376 Structural Analysis of Non-Selective Cation Channel TRPV4 using a Phase-Contrast Transmission Electron Microscope ○Hideki Shigematsu1，Takaaki Sokabe2，Makoto Tominaga2,3，Kuniaki Nagayama1,3 1Section of Nano-Structure Physiology, Okazaki Institute for Integrative Bioscience, National Institutes of Natural Sciences 2Section of Cell Signaling, Okazaki Institute for Integrative Bioscience, National Institutes of Natural Sciences 3Department of Physiological Sciences, The Graduate University for Advanced Studies

15. Cellular signal transduction 1P377 Discrimination of G-protein by analyzing physicochemical properties of N- and C- terminal regions of G-proteins ○Ganga D. Ghimire1,2，Kenichiro Imai4，Fumitsugu Akazawa3，Toshiyuki Tsuji5，Masashi Sonoyama2，Shigeki Mitaku2,3 1Venture Business Laboratory, Nagoya University 2Department of Applied Physics, Graduate School of Engeneering,Nagoya University 3Tokyo University of Agriculture and Technology, Department of Biotechnology 4Toyota Physical and Chemical Research Institute 5Frontiers of Computational Science, 21st century COE program, Nagoya University 1P378 cDNA cloning and expression analysis of signaling proteins in olfactory sensory system of Japanese common newt, Cynopus pyrrhogaster ○Masataka Takashima1，Keiko Okano2，Ken Sawada1，Tadashi Nakamura 2，Tatsuo Iwasa 1 1Dept. of Mat. Sci. and Eng., Muroran IT 2Dept. of App. Phys. And Chem., Univ. of Electro-Comm. 1P379 Expression and Purification of the Extracellular Region of Interleukin-13 Receptor α1 Chain and its Interaction with Ligand. ○Ryota Kuroki1，Eijiro Honjo1，Taro Tamada 1，Kazuhiko Arima2，Kenji Izuhara2 1Quantum Beam Science Directorate, JAEA 2Dept. of Biomolecular Sciences, Saga Medical School 1P380 Compartments and lipid microdomains in a muscle cell membrane as observed by single-molecule tracking of phospholipids ○Kenji Tanaka1，Takahiro Fujiwara2，Kenichi Suzuki2，Xiao.Y Shan1，Akihiro Kusumi2 1Dept. of Biological Science, Nagoya Univ. 2ICORP-JST, Institute for cal Sciences, Kyoto Univ. 1P381 Transient recruitment of the cytoplasmic lipid-anchored molecules at GPI-anchored receptor clusters: single-molecule detection ○Ikuko Koyama-Honda1，Ken Ritchie2，Takahiro Fujiwara1，Eriko Kajikawa1，Hideji Murakoshi1，Akihiko Yoshimura3， Akihiro Kusumi1 1Membrane Mechanisms Proj., ICORP-JST, Kyoto Univ., and Kusumi Membrane Organizer Proj., ERATO-SORST-JST, Nagoya Univ. 2Dept. of Physics, Purdue Univ. 3Inst. for Bioregulation, Kyushu Univ. 1P382 Single fluorescent molecule tracking of MARCKS on the cytoplasmic surface of the cell membrane ○Kokoro Iwasawa1，Eriko Kajikawa1，Ikuko Honda-Koyama1，Rinshi Kasai1，Ken Ritchie2，Yoshihiro Miwa 3，Akihiro Kusumi1 1Membrane Mechanisms Project, ICORP-JST, The Institute for Frontier Medical Sciences, Kyoto University 2Department of Physics, Purdue University 3Graduate School of Comprehensive Human Science, Tsukuba University 1P383 Direct estimate of the dimer fraction of a GPCR using a single fluorescent-molecule imaging ○Rinshi S. Kasai1，Eric R. Prossnitz2，Akihiro Kusumi1 1ICORP-JST, Inst. for Frontier Med. Sci., Kyoto Univ. 2Dept. of Cell Biol. and Physiol., Univ. of New Mexico 1P384 Single-molecle tracking of the dynamic remodeling of glutamate receptor clusters induced by ligand binding ○Chieko Nakada1，Hiroto Yoshida1，Rie Hasegawa1,2，Tatsuya Umeda 3，Shigeo Okabe3，Akihiro Kusumi1,2 1Institute for Frontier Medical Sciences, Kyoto University 2Membrane Mechanism Project, ICORP-JST 3Tokyo Medical and Dental University 1P385 Tetrameric hub structure of postsynaptic scaffolding protein Homer ○Mariko K. Hayashi1，Heather M. Ames1，Yasunori Hayashi1 1RIKEN-MIT Neurosci. Center, The Picower Inst. for Learning and Memory, MIT 1P386 Hierarchical Clustering of Epidermal Growth Factor Receptor in the Plasma Membrane as Observed Using Single- molecule Microscopy ○Kenji Nishida1，Toshio Yanagida1，Yasushi Sako2 1Nanobiology Laboratories, Osaka University 2Cellular Informatics Laboratory, RIKEN 1P387 Interactions between intracellular signaling molecules Ras and Raf1 analyzed by single-molecule imaging ○Kayo Hibino1，Toshio Yanagida2，Yasushi Sako1 1Discovery Res. Inst., RIKEN 2Grad. Sch. of Frontier Biosciences, Osaka Univ. 1P388 Relevance of ternary complex model to intracellular dynamics of G protein-coupled chemoattractant receptor in living Dictyostelium cells ○Yukihiro Miyanaga1，Hidekazu Kuwayama1，Toshio Yanagida1，Masahiro Ueda1 1Laboratories for Nanobiology, Graduate School of Frontier Biosciences, Osaka University 1P389 Effect of cobalt treatment on mitochondrial DNA damage in HEK293 acutely exposed to H2O2 ○Yasutomo Nomura 1，Hiroyuki Fujuwara2，Toshifumi Takayama 1，Michihiko Sato3，Eiji Takahashi4，Yasukazu Hozumi5， Zhonggang Feng2，Takao Nakamura1 1Dept. Environ. Life Sci., Yamagata Univ 2Dept. Bio-System Eng., Yamagata Univ 3Central Lab. Res. Edu., Yamagata Univ 4Dept. Physiol., Yamagata Univ 5Dept. Anat. Cell Biol., Yamagata Univ 1P390 Effect of phosphatidylserine on function and structure of the PLC-δ1 PH domain at the membrane surface ○Naoko Uekama1，Takio Sugita1，Masashi Okada1，Hitoshi Yagisawa1，Satoru Tuzi1 1Grad. Schl. Life Sci, Univ. Hyogo 1P391 Effects of nonbilayer lipid and membrane curvature on membrane association of PLC-δ1 PH domain ○Satoru Tuzi1，Akiko Hatakeyama1，Takahiro Aoki1，Naomi Tokuda1，Naoko Uekama1，Masashi Okada1，Hitoshi Yagisawa1 1Grad. Schl. Life Sci., Univ. Hyogo 1P392 The plasma membrane shuttling of CAPRI is related to regulation of mast cell activation ○Tadahide Furuno1，Rika Nakamura2，Mamoru Nakanishi1 1Sch. Pharm., Aichi Gakuin Univ. 2Grad. Sch. Pharm. Sci., Nagoya City Univ. 1P393 Effects of two phosphatases, HePTP and VHR, on mast cell activation ○Ikuko Mizuno1，Tadahide Furuno2，Naohide Hirashima1，Mamoru Nakanishi2 1Grad. Sch. Pharmaceut. Sci., Nagoya City Univ. 2Sch. Pharm., Aichi-Gakuin Univ. 1P394 Gravity-induced calcium transient devoid of amyloplast contribution may be involved in an early phase of gravitropism in Arabidopsis ○Masatsugu Toyota1，Takuya Furuichi1，Hitoshi Tatsumi1，Masahiro Sokabe1,2,3 1Dept. Physiol., Nagoya Univ. Grad. Sch. Med. 2Cell-Mechanosensing Project, SORST, JST 3Natl. Inst. Physiol. Sci., Dept. Mol. Physiol. 1P395 An attempt at imaging of single functioning synaptosomes ○Toshiaki Teratani 1，Suguru Kawato2，Yoshihiro Ohta 1 1Depatment of Biotechnology and Life Science, Graduate School of engineering, Tokyo University of Agriculture and Technology 2Department of Biophysics and Life Sciences, University of Tokyo 1P396 Reactive oxygen species modulate CDK4/cyclin D1 in differentiation of PLB-985 cells: a crucial role of NADPH oxidase ○Yoshihiro Ando1，Tomoko Suzuki 1，Wakako Hiraoka1 1Dept. of Physics, Meiji Univ. 1P397 Fluorescence Imaging of the Effects of Cyclophilin D on Mitochondrial Dysfunction ○Chisako Fujita1，Hidejiro Tazawa1，Kiyotaka Machida2，Hiroyuki Osada2，Yoshihiro Ohta 1 1Division of Biotechnology and Life Science, Tokyo University of Agriculture and Technology 2The Antibiotics Laboratory, Discovery Research Institute, Riken 1P398 Single Mitochondrion Imaging of Internal Membrane Structure Changes Daisuke Morikawa1，○Yoshihiro Ohta 1 1Tokyo Univ. of Agriculture and Technology, Div. of Biotech. and Life Sci. 1P399 Suppression of ROS production by UCP2 in neuronal cell subjected to oxidative stress ○Kenji Tomita1，Sakie Kimura1，Hitoshi Yamasita2，Masafumi Funakoshi1，Yoshihiro Ohta1 1Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology 2Department of Biomedical Sciences, Chubu University 1P400 Disturbances in chemotactic signaling by an alteration in membrane lipids ○Satomi Matsuoka1,2，Toshio Yanagida 1，Masahiro Ueda1 1Graduate School of Frontier Biosciences, Osaka University 2JSPS Research Fellow

16. Cell-cell communication 1P401 Relations between intra-cellular reaction and inter-cellular interaction for chemotactic aggregation ○Masayo Inoue1，Kunihiko Kaneko1,2 1Dept. of Basic Science, Univ. of Tokyo 2ERATO, JST 1P402 Heterogeneity of cell population in continuous culture of Escherichia coli Makoto Sadamitsu1，○Chikara Furusawa1,2，Akiko Kashiwagi1，Tetsuya Yomo1,2,3，Hiroshi Shimizu1 1Grad. School of Information Science and Technology, Osaka Univ. 2ERATO Complex Systems Biology Project, JST 3Grad. School of Frontier Biosciences, Osaka Univ. 1P403 Individual-cell-based measurement of the field potential in a cardiomyocyte by multi-electrode array with agarose microchambers ○Tomoyuki Kaneko1,2，Kensuke Kojima3，Ikurou Suzuki3，Yoshihiro Sugio3，Kenji Yasuda1 1Department of Biomedical Information, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University 2PRESTO, Japan Science and Technology Agency 3Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo 1P404 Measurement of Community Effect of Cardiomyocytes Estimated by Cell-number Dependency of Beat-Rate Fluctuation Decrease ○Kensuke Kojima1，Tomoyuki Kaneko2，Kenji Yasuda2 1Dept. of Life Sciences, Tokyo Univ. 2Dept. of Biomedical Information, Tokyo Medical and Dental Univ. 1P405 Study of Cellular Communication of Suprachiasmatic Nucleus through Synaptic Connections. ○Yoshihiro Sugio1，Hideyuki Terazono2，Ikurou Suzuki1，Akihiro Hattori2，Tomoyuki Kaneko2,3，Yasuhiko Jimbo4， Kenji Yasuda1,2 1Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo 2Department of Biomedical Information, Division of Biosystems, Institute of Biomaterials and Bioengineering, Tokyo Medial and Dental University 3PRESTO, JST 4Department of Human and Engineered Environmental Studies, Graduate School of Frontier Sciences, University of Tokyo 1P406 Role of SynCAM in the interaction between dorsal root ganglia and mast cells ○Keisuke Okamoto1，Tadahide Furuno2，Naohide Hirashima1，Mamoru Nakanishi2 1Grad. Sch. Pharmaceut. Sci., Nagoya City Univ. 2Sch. Pharma., Aichi-Gakuin Univ. 1P407 Fitness induced gene expression of chloramphenicol-resistant Escherichia coli strain ○Saburo Tsuru1，Junya Ichinose3，Akiko Kashiwagi1，Tetsuya Yomo1,2,3 1Department of Bioinformatic Engineering, Graduate School of Information Science and Technology Osaka University 2Graduate School of Frontier Biosciences, Osaka University 3Complex Systems Biology Project, ERATO, Japan Science and Technology Corporation 1P408 Structure of Exopolysaccharide from the Biofilms of Thermus aquaticus YT-1 ○Miao_Hsia Lin1，Yu_Liang Yang1，Mao_Yuan Chen2，Guang_Huey Lin3，San_San Tsay2，Shih_Hsiung Wu1 1Institute of Biological Chemistry, Academia Sinica, Taipei 115, Taiwan 2Department of Life Science and Institute of Plant Biology, National Taiwan University, Taipei 106, Taiwan 3Microbial Genetics Laboratory, Institute of Microbiology Immunology and Molecular Medicine, Tzu-Chi University, Hualien, 970, Taiwan

17. Light driven systems 1P409 Photoreaction mechanisms of two cyanobacterial BLUF domain proteins studied by low temperature spectroscopy ○Yoshimasa Fukushima1，Koji Okajima2，Masahiko Ikeuchi2，Shigeru Itoh1 1Division of Material Science, Graduate School of Science, Nagoya University 2Department of Life Science, Graduate School of Arts and Sciences, University of Tokyo 1P410 Light-induced structural changes in the BLUF domain of AppA revealed by Raman spectroscopy ○Masashi Unno1，Shinji Masuda2，Taka-aki Ono3，Seigo Yamauchi4 1Faculty of Sci. & Eng., Saga Univ. 2Grad. Sch. of Biosci. and Biotech., Tokyo Inst. of Technology 3College of Engin., Ibaraki Univ. 4IMRAM, Tohoku Univ. 1P411 Photoinduced Electron Transfer in Glucose Oxidase: A Picosecond Time-resolved Ultraviolet Resonance Raman Study ○Akiko Fujiwara1，Yasuhisa Mizutani2 1Grad. Schl. of Sci. and Tech., Kobe Univ. 2Grad. Schl. of Sci., Osaka Univ. 1P412 Identification of the N-H stretch of Asn1008 by FTIR spectroscopy in the LOV2 domain of Adiantum Phytochrome3 ○Tatsuya Iwata1，Dai Nozaki1，Satoru Tokutomi2，Hideki Kandori1 1Department of Materials Science and Engineering, Nagoya Institute of Technology 2Department of Biological Science, Graduate School of Science, Osaka Prefecture University 1P413 Role of Phe-1010 in the light-induced structural changes of LOV2 domain of Adiantum Phytochrome3 ○Atsushi Yamamoto1，Tatsuya Iwata1，Satoru Tokutomi2，Hideki Kandori1 1Department of Materials Science and Engineering, Nagoya Institute of Technology 2Department of Biological Science, Graduate School of Science, Osaka Prefecture University 1P414 Molecular Dynamics Study of Hydrated Water Behavior in Photoactive Yellow Protein ○Taku Mizukami1,3，Ayumu Sugiyama2，Hidemi Nagano2,3 11School of Materials Science, Japan Advanced Institute of Science and Technology 2Division of Mathematical and Physical Science, Graduate School of Natural Science and Technology, Kanazawa University 3HJK center 1P415 Spectral tuning mechanism and theoretical prediction of absorption maximum of photoactive yellow protein ○Kazutomo Kawaguchi1，Takahisa Yamato1,2 1Graduate School of Science, Nagoya University 2CREST, JST 1P416 A theoretical study on the proteinquake of photoactive yellow protein ○Kana Koike1，Takehisa Yamato1,2 1Graduate School of Science 2CREST,JST 1P417 The proton transfer reaction in the Photocycle of Photoactive Yellow Protein ○Motoshi Kamiya1，Shinji Saito2，Iwao Ohmine1 1Dept. of Chemistry, Nagoya Univ. 2Institute for Molecular Science 1P418 FTIR study of Internal Water Molecules in the Schiff Base Region of Proteorhodopsin ○Daisuke Ikeda1，Yu j i Fu ru tani 1，Hideki Kandori1 1Department of Materials Science and Engineering, Nagoya Institute of Technology 1P419 Halide-Bound D212N Mutant of Bacteriorhodopsin Mikihiro Shibata1，Maiko Yoshitsugu1，Noriko Mizuide1，Kunio Ihara2，○Hideki Kandori1 1Nagoya Institute of Technology 2Nagoya Univ. 1P420 Studies on reaction dynamics of halorhodopsin during chloride ion pumping ○Keiichi Inoue1，Megumi Kubo2，Makoto Demura2，Naoki Kamo3，Masahide Terazima1 1Dept. of Chemistry, Grad. School of Science, Kyoto Univ. 2Div. of Biological Science, Grad. School of Science, Hokkaido Univ. 3Faculty of Pharmaceutical Sciences, Grad. School of Pharmaceutical Siences, Hokkaido Univ. 1P421 FTIR Study of the O Intermediate in the Complex between pharaonis Phoborhodopsin and Its Cognate Transducer ○Motohiro Ito1，Yuji Furutani1，Masayo Sumii1，Naoki Kamo2，Hideki Kandori1 1Department of Materials Science and Engineering, Nagoya Institute of Technology 2Graduate School of Pharmaceutical Sciences, Hokkaido University 1P422 Converting a light-driven ion pump (bacteriorhodopsin) into a photosensory receptor ○Yuki Sudo1，John Spudich1 1The University of Texas at Houston 1P423 Local Conformation and Dynamics Changes in the vicinity of the Retinal in Photoactivated pharaonis phoborhodopsin by Solid-State NMR ○Takudo Nishio1，Izuru Kawamura1，Hazime Saito2，Naoki Kamo3，Akira Naito1 1Graduate School of Engineering, Yokohama National University 2Center for Quantum Life Sciences, Hiroshima University 3Laboratory of Biophysical Chemistry, Graduate School of Pharmaceutical Sciences 1P424 Pressure induced isomerization of retinal and dynamics change of protein on bacteriorhodopsin in the dark by Fast Magic Angle Spinning NMR ○Izuru Kawamura1，Yoshiaki Degawa1，Satoru Yamaguchi2，Katsuyuki Nishimura3，Satoru Tuzi2，Hazime Saito4，Akira Naito1 1Grad. Sch. Eng., Yokohama Natl. Univ. 2Dept. Life Sci., Univ. Hyogo 3Inst. Mol. Sci. 4Dept. Quantum Life Sci., Hiroshima Univ. 1P425 Structure analysis of Tyr residues with retinal isomerization in bacteriorhodopsin by solid state NMR. ○Naoki Kihara1，Izuru Kawamura1，Satoru Tuji2，Hajime Saito3，Akira Naito1 1Grad. Sch. Eng., Yokohama Natl. Univ. 2Dept. Life Sci. Univ. Hyogo 3Dept. Quantum Life Sci. Hiroshima Univ. 1P426 Local structure analysis of Tyr residues is located in trans-membrane site of bacteriorhodopsin by solid state high resolution NMR. ○Naoki Kihara1，Izuru Kawamura1，Satoru Tuji2，Hajime Saito3，Akira Naito1 1Sch Eng, Yokohama Na 2Sch, Hyogo Univ. 3Sch, Hiroshima Univ. 1P427 Local structural change of bacteriorhodopsin accompanied with the removal of retinal as studied by solid state NMR ○Junko Tanabe1，Masato Ohmine1，Izuru Kawamura1，Naoki Kihara1，Satoru Tuzi2，Hazime Saito2,3，Akira Naito1 1Grad. Sch. Eng., Yokohama Natl. Univ. 2Dept. Life Sci., Univ. Hyogo 3Dept. Quantum Life Sci., Hiroshima Univ. 1P428 Inter- and Intra-Molecular Interaction Involved in Photobleaching Bacteriorhodopsin Reconstituted in DMPC Liposome ○Rumi NEGISHI1，Masashi SONOYAMA1，Shigeki MITAKU1 1Nagoya University 1P429 Identification of amino acid residues responsible for the helical movement of metabotropic glutamate receptor in the activation process ○Masataka Yanagawa1，Takahiro Yamashita1，Akihisa Terakita1,2，Yoshinori Shichida1 1Dept. of Biophys., Grad. Sch. of Sci., Kyoto Univ. and CREST-JST, Kyoto 2Present Address: Dept. of Biol., Grad. Sch. of Sci., Osaka City Univ., Osaka 1P430 Expression and characterization of melanopsin ○Akihisa Terakita1,3，Mitumasa Koyanagi1，Hisao Tsukamoto2,3，Yoshinori Shichida2,3 1Department of Bio- and Geosciences, Graduate School of Science, Osaka City Uiversity 2Department of Biophysics, Graduate School of Science, Kyoto Uiversity 3CREST/JST 1P431 Mutational analyses of active state of bistable rhodopsins. ○Hisao Tsukamoto1,2,3，Akihisa Terakita2,3，Mitsumasa Koyanagi2,3，Yoshinori Shichida1,2 1Department of Biophysics, Graduate School of Science, Kyoto University 2Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency 3Department of Bio- and Geosciences, Graduate School of Science, Osaka City University. 1P432 Insights into the role of the covalent bond between rhodopsin and the chromophore. ○Takesi Matsuyama H1，Hiroo Imai2，Yoshinori Shichida1 1Department of Biophysics, Graduate School of Science, Kyoto University and CREST-JST, Kyoto 606-8502 2Dept. of Cell. Mol. Biol., Primates Res. Inst., Kyoto University, Inuyama, Aichi 484-8506 1P433 Regulation of the photoisomerization efficiency in visible and UV-absorbing visual pigments ○Kei Tsutsui1,2，Hiroo Imai1,2,3，Yoshinori Shichida1,2 1Department of Biophysics, Graduate School of Science, Kyoto University, Kyoto 606-8502 2Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency, Kyoto 606-8502 3Present Address: Department of Cellular and Molecular Biology, Primate Research Institute, Inuyama, Aichi 484-8506 1P434 ES1 : an abundant protein specifically expressed in cone inner segments of the carp retina ○Yuhki Komatsu1，Shuji Tachibanaki1,2，Satoru Kawamura1,2 1Grad. Sch. of Frontier Biosciences, Osaka Univ. 2Dept. of Biology, Grad. Sch. of Sci., Osaka Univ. 1P435 High cGMP synthetic activity in cones: the mechanism of rapid recovery in a cone photoresponse ○Norihiko Takemoto1，Yoshie Shimauchi-Matsukawa1，Shuji Tachibanaki1,2，Satoru Kawamura1,2 1Grad. Sch. of Frontier Biosciences, Osaka Univ. 2Dept. of Biology, Grad. Sch. of Sci., Osaka Univ. 1P436 Structural Changes in the L2-intermediate of pharaonis Halorhodopsin Studied by FTIR Spectroscopy ○Mikihiro Shibata1，Makoto Demura2，Hideki Kandori1 1Department of Materials Science and Engineering, Nagoya Institute of Technology 2Division of Biological Sciences, Graduate School of Science, Hokkaido University 1P437 Pump-dump fluorescence spectroscopy for photoactive yellow protein ○Yasuo Kanematsu 1，Ryosuke Nakamura1，Norio Hamada1，Hideki Ichida1，Fumio Tokunaga2 1JST-CREST, VBL-CASI, Osaka Univ. 2JST-CREST, Dept. of Earth and Space Sci., Osaka Univ. 1P438 Enhancement of photoreaction in Photoactive Yellow Protein by infrared laser pulse irradiation ○Suguru Ida1，Hideki Ichida2，Ryosuke Nakamura3，Norio Hamada3，Yasuo Kanematsu 2,3，Fumio Tokunaga1,3 1Grad. school of Sci, Dept. of biology, Osaka Univ. 2VBL-CASI Osaka Univ. 3JST-CREST 1P439 Kinetics of major conformational change of PYP ○Yuji Hoshihara 1，Yasushi Imamoto 2，Mikio Kataoka2，Fumio Tokunaga3，Yoshifumi Kimura 4，Masahide Terazima1 1Dept. of Chem., Grad. Sch. of Sci., Kyoto Univ. 2Grad. Sch. of Material Sci., Nara Inst. of Sci. and Tech. 3Dept. of Earth and Space Sci., Grad. Sch. of Sci., Osaka Univ. 4International innovation center, Kyoto Univ. 1P440 Equilibrium between monomer and dimer forms of phototropin1-LOV2 domain ○Yusuke Nakasone1，Takeshi Eitoku1，Daisuke Matsuoka2，Satoru Tokutomi2，Masahide Terazima1 1Graduate school of science, Kyoto University 2RIAST, Osaka Prefecture University 1P441 Color Tuning of the Rhodopsin Chromophore Using Clay ○Kazutomo Ido1，Yuji Furutani 1，Masako Sasaki2，Makoto Ogawa3，Hideki Kandori1 11Department of Materials Science and Engineering, Nagoya Institute of Technology 2Research Institute of Science and Technology, Tokai University 3Department of Earth Sciences, School of Education, Waseda University

18. Neuro-infomatics 1P442 Effect of reliability in the Bayesian inference model of neural networks in the presence of multiple perceptions ○Seigo Maekawa1，Kenichi. L. Ishikawa1,2 1School of Engineering, University of Tokyo 2JST 1P443 Spatial Reward Learning Dynamical System Model ○Adam Ponzi1 1RIKEN Brain Science Institute 1P444 The correlation between the environmental adaptability and the diversity of the time window length of spatio-temporal response selectivity ○Hiroyuki Ohta1，Yukio Gunji2 1Graduate School of Science and Technology, Kobe Univ. 2Faculty of Science, Kobe Univ. 1P445 A brain activity to control switching among perceptual states for Ambiguous Apparent Motion ○Masanori Shimono1，Tsunehiro Takeda2 1Tokyo Univ. 2Tokyo Univ.

19. Behavior science 1P446 Bilateral Cerebellar Ablation Does not Impair Trace Eyeblink Conditioning in Mice ○Takayuki Kotoku1，Takahiro Horiuchi1，Shigenori Kawahara1 1Graduate School of Pharmaceutical Sciences, The University of Tokyo 1P447 Depth perception in Plaid pattern motion and in Crossed barber-pole Illusion ○Takashi Nakamura1 1Faculty of Humanities, Niigata University 1P448 Exploratory behavior in pill bugs whose antennae wear the artificial attachments. ○Tohru Moriyama1，Tetsuro Takeda2 1Dept. Complex Syst., Future Univ.-Hakodate 2Grad. Sch. Syst. Infom. Sci., Future Univ.-Hakodate 1P449 Response of C.elegans to low-temperature stress ○Masakazu Hayashi1，Taeko Oguma 2，Norio Murase1,2，Hisako Amino3，Kiyoshi Kita3 1Graduate School of Advanced Science and Technology, Tokyo Denki University 2Graduate School of Science and Engineering, Tokyo Denki University 3Graduate School of Medicine, the University of Tokyo 1P450 The temporal and spacial scaling rule involved in the locomotion of ant and mouse ○Kazumitsu Hanai1，Mamiko Ozaki2，Daigo Yamauchi2，Chihiro Yokoyama3，Yasuhito Nakatomi3，Kenji Fukui3 1Dept. Physics, Kyoto Pref. Univ. Med. 2Dept. App. Biol., Kyoto Inst. Tech. 3Dept. Psychiatry, Kyoto Pref. Univ. Med. 1P451 The true slime mold shows the response to periodic environmental change ○Tetsu Saigusa1，Toshiyuki Nakagaki1 1Hokkadio University

20. Radiation biology 1P452 An energy-tunable X-ray microbeam irradiation system at the Photon Factory ○Katsumi Kobayashi1，Noriko Usami1，Hiroshi Maezawa2，Tohru Hayashi3，Kotaro Hieda4，Kaoru Takakura5，Yoshiya Furusawa6 1Photon Factory, IMSS, KEK 2Sch. Health Science, Univ. Tokushima 3Hayashi So-ken Inc. 4Dep. Life Sci., Coll. Sci., Rikkyo Univ. 5Coll. Liberal Arts, Int. Christian Univ. 6HIMAC, Natl. Inst. Radiol. Sciences 1P453 X-ray microbeam system for irradiation of living cells ○Takahiro Kuchimaru1，Fuminobu Sato1，Kikuo Shimizu2，Yushi Kato1，Toshiyuki Iida1 1Division of Electrical, Electronic and Information Eng., Osaka Univ. 2Radioisotope Research Center, Osaka Univ. 1P454 Localization of DNA double-strand break repair proteins in the target irradiated cell nuclei by synchrotron X-ray microbeam ○Masanori Tomita1，Munetoshi Maeda2，Noriko Usami3，Yoshihisa Matsumoto 4，Katsumi Kobayashi3 1Low Dose Radiat. Res. Cent., Centl. Res. Inst. Electric Power Industry 2Dept. Materials Structure Sci., GUAS. 3Photon Factory, IMSS, KEK 4Dept. Rad. Res., Cent. Disease Biol. & Integrative Med., Grad. Schl. Med., Univ. Tokyo 1P455 Comparison of the survival fractions of cells irradiated with X-ray microbeams of different sizes ○Munetoshi Maeda1，Noriko Usami1,2，Katsumi Kobayashi1,2 1Dept. Materials Structure Sci., GUAS. 2KEK-PF 1P456 Hematopoietic stem cell dynamics under low dose-rate gamma-irradiation ○Kensuke Otsuka1，Hiroshi Tauchi2，Kazuo Sakai3，Takao Koana1 1Low Dose Radiat. Res. Center, Cetrl. Res. Inst. Electric Power Industry 2Department of Environmental Sciences, Faculty of Sciece, Ibaraki University 3Research Center for Radiation Protection, National Institute of Radiological Sciences 1P457 Radiation inhibits protein downregulation by RNA interference and antisense oligodeoxynucleotides in human breast cancer cells. ○Tetsuo Nakajima1，Mitsuru Nenoi1 1Research Center for Radiation Safety, Natl. Inst. Radiol. Sci. 1P458 Cu2+ release and inactivation of tyrosinase induced by 1MHz ultrasound ○Tsukasa Takahashi1，Takashi Kondo2，Wakako Hiraoka1 1Dept. of Physics, Meiji Univ. 2Dept. of Radiorogical, Toyama Univ.

21. Development and differentiation 1P459 A regional gradient of intrinsic rhythmicity depicted in embryonic cultured multiple-hearts ○Tetsuro Sakai2，Kohtaro Kamino1 1Dept. of Physiology, Tokyo Medical and Dental Univ. Graduate Sch. and Faculty of Medicine 2Dept. of Physiology, Univ. of the Ryukyus Sch. of Medicine 1P460 Cell division of primary stromal-vascular cells during adipose differentiation ○Masafumi Nagayama1，Tsutomu Uchida1，Toshio Taira 2，Kyoko Shimizu2，Masato Sakai2，Kazutoshi Gohara1 1Division of Applied Physics, Graduate School of Engineering, Hokkaido University 2Primary Cell Co., Ltd. 1P461 The homeobox-containing protein, Hbx3 is implicated in tip formation and prespore differentiation in Dictyostelium discoideum ○Ji-Sun Kim1，Chang-Hoon Choi1，Sun-Young Jeong1，Sa-Ouk Kang 1 1Laboratory of Biophysics, School of Biological Sciences. and Institute of Microbiology, Seoul National University 1P462 Cancel

1P463 On rigorous conditionsfor high cell-type diversity in virtue of algebraic approach ○Hiroshi YOSHIDA1，Hirokazu ANAI2，Katsuhisa HORIMOTO3 1Inst. of Med. Sci., Tokyo Univ. 2 IT Core Lab., FUJITSU LAB. LTD./CREST, JST. 3CBRC, AIST

22. Genome biology 1P464 Structural and functional diversities of enzymes with the common catalytic domain ○Keiko Tan'ya1，Motonori Ota2，Ken Nishikawa3 1Grad. Sch. of Biosci&Biotech, Tokyo Tech. 2GSIC, Tokyo Tech. 3CIB-DDBJ, NIG 1P465 Novel Genomic Analysis Tool based on the Scanning Probe Microscope ○Kimiko Yamamoto1，Seigo Kuwazaki1，Kazumi Tsukamoto2，Satoshi Akanuma2，Yoshitaka Suetsugu1，Junko Narukawa1， Kazuei Mita1，Toshio Ohtani2，Shigeru Sugiyama2 1National Institute of Agrobiological Sciences 2National Food Research Institute, NARO 1P466 Successive dissection and recovery of chromosome nano-fragments and direct BAC mapping onto chromosomes by scanning probe microscopy ○Shigeru Sugiyama1，Kazumi Tsukamoto1，Takeshi Yamauchi1，Seigo Kuwazaki2，Tomoyuki Yoshino3，Junichi Wakayama1， Satoshi Akanuma1，Megumi Sasou1，Hirokazu Takahashi1，Kimiko Yamamoto2，Toshio Ohtani1 1National Food Research Institute, NARO 2National Institute of Agrobiological Sciences 3Prefectural University of Hiroshima

23. Bioinformatics, genomics and proteomics (I) 1P467 Physicochemical properties of secondary structures around non-charged amino acid segments ○Naoyuki Asakawa1，Kenichiro Imai1,2，Shigeki Mitaku1 1Nagoya University, Graduate School of Engineering, Department of Applied Physics 2Toyota Physical and Chemical Research Institute 1P468 Proteins with charge periodicity of 28 residues from total genomes ○Runcong Ke1，Shigeki Mitaku1 1Dept. of Applied Physics, Nagoya Univ. 1P469 Characteristic cellular localization of proteins which have charge periodicity of 28 residues ○Noriyuki SAKIYAMA1，Runcong KE1，Shigeki MITAKU1 1Department of Applied Physics, Graduate School of Engineering, Nagoya University 1P470 Amino acid sequence analysis of motor protein by physical finger print method ○Hideki Tanizawa1，Shigeki Mitaku1 1Dept. of Applied Physics, Nagoya Univ. 1P471 Prediction of Secondary Structure Breaker in Soluble Proteins ○Kenichiro Imai1,2，Shigeki Mitaku2 1Toyota Phys. and Chem. Res. Inst. 2Dept. of Appl. Phys., Grad. Sch. of Eng., Nagoya Univ. 1P472 Domain linker prediction using a Support Vector Machine(SVM) ○Teppei Ebina1，Takao Arai1，Hiroyuki Toh2，Yu taka Ku rod a 1 1Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology 2Medical Institute of Bioregulation, Kyushu University 1P473 Probabilistic description of profile-profile comparison for remote homology detection ○Ryotaro Koike1,2，Motonori Ota1，Akinori Kidera3 1Global Sci. Info. & Comput. Center, Tokyo Inst.of Tech. 2BIRD, JST 3International Grad. Sch. Arts & Sci.,Yokohama City Univ. 1P474 Automatic extraction of conserved region from alignment based on protein structure ○Shinsuke Yamada1，Koutarou Yamada1，Hayato Yamana1，Tamotsu Noguchi2 1Dept. of Computer Science, Waseda Univ. 2Computational Biology Research Center, AIST 1P475 Predicting protein disordered region from classified data ○Hiromi Arai1，Masayuki Yamamura2 1Department of Computational Intelligence and Systems Science, Tokyo Institute of Technology 2Department of Computational Intelligence and Systems Science, Tokyo Institute of Technology 1P476 Intrinsically Disordered Regions of Human Membrane Proteins ○Yoshiaki Minezaki1，Ken Nishikawa1 1CIB-DDBJ Center, National Institute of Genetics 1P477 Analysis of transmembrane helical regions of G-protein-coupled receptors (GPCRs) for a novel prediction system ○Toshiyuki TSUJI1,2，Shigeki MITAKU1,2 1Dept. of Applied Physics, Graduate School of Engineering Nagoya Univ. 221 Century COE Program "Frontiers of Computational Science" Graduate School of Engineering Nagoya Univ. 1P478 Sequence and structure features of GPCRs, which are effective to determine the GPCR and G-protein binding selectivity ○Makiko Suwa1，Takahiko Muramatsu2 1Computational Biology Research Center, National Institute of Advanced Industrial Science and Technology 2Nara Institute of Science and Technology 1P479 A possible effect of alternative splicing on multiple regions of a single protein ○Masafumi Shionyu1，Kei Yura2，Mitiko Go1,3 1Fac. Bio-Sci., Nagahama Inst. Bio-Sci. Tech. 2Quantum Bioinfo., JAEA 3Ochanomizu Univ. 1P480 Database analysis of S•••O and S•••N interactions for phospholipase A2 ○Michio Iwaoka1，Noriyoshi Isozumi1 1Department of Chemistry, School of Science, Tokai University 1P481 Real-time Structure Based Queries at the Protein Data Bank japan ○Daron M. Standley2,4，Hiroyuki Toh3，Haruki Nakamura1 1Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Osaka University, Osaka 2Japan Science and Technology Agency, Institute for Bioinformatics Research and Development (BIRD) 3Medical Institute of Bioregulation, Kyushu University, Kyushu 4Center for Advanced Medical Engineering and Informatics, Osaka University, Osaka 1P482 Structural based analysis of protein-sugar binding sites ○Clara Shionyu-Mitsuyama1，Kengo Kinoshita2，Akinori Kidera3，Haruki Nakamura1 1IPR, Osaka Univ. 2IMS, Tokyo Univ. 3Grad. Sch. of Art and Sciences, Yokohama City Univ. 1P483 Computational analyses of amino acid residue propensity in protein-RNA interfaces and prediction methods for the interfaces Oanh T.P. Kim1，○Kei Yura1,2，Nobuhiro Go3 1Quantum Bioinf., CCSE, JAEA 2CREST, JST 3Comp. Biol., Quantum Beam, JAEA 1P484 Predicted Protein-Protein Interaction Networks based on Homology-Modeled Complex Structures ○Naoshi Fukuhara1，Takeshi Kawabata1，Nobuhiro Go2 1Grad. Sch. of Infor. Sci., Nara Inst. of Sci. and Tech. 2CCSE, JAEA 1P485 Efficiency of the empirical rules of ligand-protein interaction automatically processed from Protein Data Bank ○Mihoko Saito1，Tsuyoshi Shirai1 1Department of Bioscience, Nagahama Institute of BioScience and Technology 1P486 Prediction of Protein-Protein Interaction Sites Using Evolutionary and Structural Information ○Masanori Kakuta1，Shugo Nakamura1，Kentaro Shimizu1 1Graduate School of Agricultural and Life Sciences, The University of Tokyo 1P487 Improvement in Software for Kinetics Analyses of Protein-Ligand Interaction ○Masaki Yamamura1，Takuma Shiraki 2，Takashi S. Kodama3，Tatsuo Nakagawa4，Natsuhiro Ichinose1，Osamu Gotoh1 1Department of Intelligence Science and Technology, Graduate School of Informatics, Kyoto University 2Institute for Protein Research, Osaka University 3Department of Medical Genetics, Osaka University Medical School 4UNISOKU CO., LTD. 1P488 Analysis of interaction profiles between calmodulin and various target molecules by a protein-protein interaction simulation ○Nobuyuki Uchikoga1,2，Takatsugu Hirokawa2 1Japan Biological Informatics Consortium 2Computational Biology Research Center

24. Mathematical biology 1P489 Mathematical modeling of gene interactions associated with Wnt signaling pathway in colorectal carcinoma ○Yoshimi Naruo 1，Kaoru Mogushi1，Yoh Iwasa2，Hiroshi Tanaka1 1Department of Bioinformatics, Tokyo Medical and Dental University 2Department of Biology, Faculty of Sciences, Kyushu University 1P490 A Mathematical Model of Cell Size Homeostasis ○Benjamin Pfeuty1，Kunihiko Kaneko1 1Department of Pure and Applied Sciences, University of Tokyo. 1P491 Mathematical modeling for pattern formation of dendrite ○Kaoru Sugimura1，Tadashi Uemura 1，Atsushi Mochizuki2 1Kyoto University 2National Institute for basic biology 1P492 Neural Circuit and Behavioral Criteria of C. elegans ○Yuishi Iwasaki1 1Department of Intelligent System Engineering, Ibaraki University 1P493 Competitive localization of molecules in mass-conserved systems ○Shuji Ishhihara1，Mikiya Otsuji2，Atsushi Mochizuki1 1National Institute for Basic Biology 2Faculty of Medicine, University of Tokyo 1P494 Emergence of Morphological Order in the Network Formation of Physarum Plasmodium ○Tomohiro Shirakawa 1，Yukio-P. Gunji1,2 1Grad. School of Earth and Planetary Science, Kobe Univ. 2Dept. of Earth and Planetary Sciences, Kobe Univ. 1P495 Solving the shortest path problem by Physarum solver - Modeling of the Adaptive Network of True Slime Mold ○Atsushi Tero1，Ryo Kobayashi2，Toshiyuki Nakagaki3 1Hokkaido Univ. 2Hiroshima Univ. 3Hokkaido Univ. 1P496 Predicting regulation of the phosphorylation cycle of KaiC clock protein using mathematical models Hisako Takigawa-Imamura1，○Atsushi Mochizuki1 1National Institute for Basic Biology 1P497 Modeling of the cyanobacterial KaiC phosphorylation cycle in vitro Mitsumasa Yoda1，○Kohei Eguchi1，Tomoki Terada 1，Masaki Sasai1 1Dept. of Computational Science and Engineering, Graduate School of Engineering, Nagoya Univ. 1P498 Phenomenological Study for the Stability of a Microbial Community ○Masashi Tachikawa1 1ERATO Complex Systems Biology Project, JST

25. New methods and tools (I) 1P499 Physicochemical characterization of nanopipette electrodes for developing real-time in vivo biosensors ○Senkei Umehara1,2，Miloslav Karhanek2，Ronald W. Davis2，Kenji Yasuda2,3，Nader Pourmand2 1Dept Life Sci, Grad Sch Arts Sci, Univ Tokyo 2Stanford Genome Technology Center, Stanford Univ 3Inst Biomed Bioeng, Tokyo Med Dent Univ 1P500 Studies of metal ion interactions with calmodulin using Dual Polarization Interferometry (DPI) ○Yoji Yoshimi 1,2，Toshifusa Toda 2 1Marubun Corporation 2Tokyo Metropolitan Institute of Gerontology 1P501 A nanoprobe method for detecting mRNAs in individual living cells ○Yuji Kunitomi1，Hironori Uehara1，Atushi Ikai1，Toshiya Osada1 1Tokyo Institute of Technollogy Department of Bioscience 1P502 3-D measurement of the nanometer displacement of microspheres under optical microscope ○Naoki Noda1，Shinji Kamimura1 1The University of Tokyo 1P503 Quantification of sizes of gold nano-particles on a cell surface by using curvature-reconstruction-method of the atomic force microscopy ○Hyonchol Kim1，Koudai Oikawa2，Naoya Watanabe3，Masatsugu Shigeno3，Yoshiharu Shirakawabe3，Kenji Yasuda1,2 1Inst. of Biomaterials and Bioengineering, Tokyo Medical and Dental Univ. 2Graduate School of Arts and Sciences, The Univ. of Tokyo 3SIINT Inc. 1P504 Observation of biological samples at high resolution by high-speed FM-AFM ○Naohisa Takahashi1，Hayato Yamashita1，Masaaki Taniguchi1，Noriyuki Kodera1，Takayuki Uchihashi1,2，Toshio Ando1,2 1Dept. of Physics, Kanazawa Univ 2CREST/JST 1P505 Detection of thermal response associated with intracellular calcium increase induced by ionomycin in single HeLa cell Vadim Tseeb1，○Madoka Suzuki2，Shin'ichi Ishiwata1,2 1Dept Phys, Sch Sci/Eng, Waseda Univ 2ASMeW, Waseda Univ 1P506 Spatial analysis of stretch-induced tyrosine phosphorylation using cell-adhesion-patterned endothelial cells ○Akira Yamada1，Yuki Katanosaka1，Tomoyo Komatsu1，Tomohiko Suemori1，Masahiro Kishio1，Bao Jinhua1，Satoshi Mohri1，Keiji Naruse1 1Dept. of Cardiovasc. Physiol., Okayama Univ. 1P507 Fluorescence Correlation Spectroscopy using a New Single-Element Aspheric Objective Lens ○Tsuyoshi Sonehara1，Takashi Anazawa1，Kenko Uchida1 1Hitachi, Ltd., Central Research Laboratory 1P508 Detection of prion protein immune complex using Fluorescence Correlation Spectroscopy and Fluorescence Cross- Correlation Spectroscopy ○Fumihiko Fujii1，Motohiro Horiuchi2，Masayoshi Ueno3，Hiroshi Sakata1，Issei Nagao1，Mamoru Tamura1，Masataka Kinjo1 1Laboratory of Biophysics, RIES, Hokkaido Univ. 2Laboratory of Prion Diseases, Graduate School of Veterinary Medicine, Hokkaido Univ. 3Obihiro Research Laboratory, Fujirebio Inc. 1P509 A novel method to quantify the nucleic acid by fluorescence correlation spectroscopy coupled with template directed DNA photoligation ○Issei Nagao1，Kenzo Fujimoto2，Masataka Kinjo3 1Japan Biological Information Research Center, AIST Bio-IT Researcn Building 2The School of Material Science (Japan) Advanced Institute of Science and Technology 3Hokkaido Univ. Research Institute for Electronic Science 1P510 FCS measurement in rat liver - beyond the cell biology ○Yuka Saito 1，Shinya Horita1，Mamoru Tamura1，Masataka Kinjo1 1Laboratory of Supramolecular Biophysics, Reserch Institute for Electronic Science, Hokkaido University 1P511 Kinetic analysis of bacteriorhodopsin photocycle by transforming time-resolved FTIR spectroscopic data into a 2D- lifetime distribution ○Victor A. Lorenz Fonfria1，Hideki Kandori1 1Dept. of Materials Science and Engineering, Nagoya Institute of Technology 1P512 Fast backbone amide assignment of 15N-HSQC spectrum ○Seongmu Bak1，Masakatsu Kamiya1,2，Yoshitaka Umetsu1，Tomoyasu Aizawa1,2，Takahide Kouno3，Mineyuki Mizuguchi3， Yasuhiro Kumaki1，Makoto Demura2，Keiichi Kawano1 1Grad. Sch. of Sci., Hokkaido Univ. 2Grad. Sch. of Life Sci., Hokkaido Univ. 3Fac. of Pharmaceut. Sci., Toyama Univ. 1P513 Solid-State 17O NMR of Biological Molecules ○Kazuhiko Yamada1，Miwako Asanuma1，Toshio Yamazaki1，Hiroshi Hirota1 1RIKEN Genomic Sciences Center 1P514 Potential Measurement of Lipid Bilayer Membrane by Microfluidic FET Device ○Yoshihiko Kuwana1，Katsura Kojima1，Yasushi Tamada 1 1Nat. Inst. of Agrobiol. Sci. 1P515 Development of a High-Sensitivitive, Multi-Frequency EPR system as a New Tool for Research on Metalloproteins with Integer Electron Spin ○Haruhiko Yashiro1，Takanari Kashiwagi1，Masaki Horitani2，Hiroshi Hori2，Masayuki Hagiwara1 1KYOKUGEN, Osaka Univ. 2Dept. of Bioeng, Osaka Univ. 1P516 Ab initio calculation of NMR chemical shifts of large molecules by FMO method Qi Gao1,2，○Satoshi Yokojima1，Takao Kobayashi1，Shinichiro Nakamura1 1Mitsubishi Chemical Group Science and Technology Research Center, INC. and CREST-JST 2Department of Biomolecular Engineering, Tokyo Institute of Technology 1P517 Direct Observation of Intracellular Materials Using a Phase Contrast Transmission Electron Microscope (TEM). ○Koji Nitta1，Hideki Shigematsu1，Yoshiyuki Fukuda2，Kuniaki Nagayama1,2 1 Laboratory of Section of Nano-Structure Physiology, Okazaki Institute for Integrative Biosciences, 2School of Life Science, The Graduate University for Advanced Studies 1P518 Light scattering can detect the thermal-dependent conformational changes of proteins. ○Kohei Shiba1，Kyoko Ogasahara2，Koji Inaka3，Shigeru Sugiyama3，Tomomitsu Hatakeyama4，Atsushi Nakagawa2 1Scientific Inst.Business Div., Sysmex Corporation 2Institute for Protein Research, Osaka University 3Mol Logics Inc. 4Faculty of technology, Nagasaki University 1P519 Nanosecond responses of proteins to ultra-high temperature pulses ○Bradley C. Steel1，David R. McKenzie1，Marcela M. M. Bilek1，Neil J. Nosworthy1，Cristobal G. dos Remedios2 1School of Physics, The University of Sydney 2Bosch Institute, The University of Sydney 1P520 Construction of selenomethionyl protein expression system by Pichia pastoris for the protein crystallography. ○Toshihiko Kitajima1，Yasunori Chiba1，Yoshifumi Jigami1 1Research Center for Glycoscience, AIST 1P521 Manipulation of protein microcrystal using a laser tweezers for X-ray crystallography ○Takaaki Hikima 1，Shin Muraoka2，Masaki Yamamoto1,2 1RIKEN SPring-8 Center 2JASRI/SPring-8 1P522 Fabrication of freezing apparatus with controlling temperature and humidity for electron cryo-microscopy ○Chie Hirai1，Takuo Yasunaga1 1Department of Bioscience and Bioinfomatics, Computer Science and System Engineering, Kyushu Institute of Technology Graduate School 1P523 Dvelopment of novel automated determination of contrast transfer function of electron microscopy ○Motoki Imamura1，Takuo Yasunaga1 1Department of Bioscience and Bioinformatics, Graduate School of Computer Science and System Engineering, Kyushu Institute of Technology 1P524 Identification of surface structure and orientation of immobilized protein with TOF-SIMS ○Satoka Aoyagi1 1Shimane University

26. Single molecule biophysics 1P525 Designing a mutant F1-ATPase for easy and rapid single molecule analysis. ○Mariko Nakamura1，Hiroshi Ueno1，Hiromi Imamura1，Hiroyuki Noji1 1ISIR , Osaka Univ. 1P526 Single-molecule analysis of F1-motor loaded with nonhydrolyzable substrate ○Ryo Fujisawa1，Daichi Okuno1，Hiroyuki Noji1 1ISIR, Osaka univ, 1P527 Does the forcible rotation of F1 motor enhance ATP hydrolysis? ○Yuko Iko 1，Takako Akamatsu1，Shouichi Sakakihara1，Kazuhito_V. Tabata1，Hiroyuki Noji1 1ISIR, Osaka Univ. 1P528 Development of the Single Molecule Imaging System of the Fo Motor ○Hiroshi Ueno1，Kazuhito Tabata1，Toshiharu Suzuki 2,3，Toru Ide4，Masasuke Yoshida2,3，Hiroyuki Noji1 1ISIR, Osaka Univ. 2Chemical Resources Laboratory, Tokyo Tech 3ATP System Project, ERATO, JST 4Graduate School of Frontier Biosciences, Osaka Univ. 1P529 Rotation rate of F1-ATPase increases from 10 to 2000 over the temperature range of 5º-65ºC ○Shou Furuike1，Kengo Adachi1，Megumu Shio2，Naoyoshi Sakaki3，Rieko Shimo-Kon1，Hiroyasu Itoh4,5，Masasuke Yoshida 6，Kazuhiko Kinosita, Jr.1 1Fac.of Sci. and Eng, Waseda Univ. 2JST ERATO ATP system 3Inst. of Industrial Sci, Univ. of Tokyo 4Tsukuba Res. Lab. 5JST CREST Soft-Nano Machine 6Chemical Resources Lab. Tokyo Tech. 1P530 Regulation of rotation of cyanobacterial F1 ATPase ○Hiroki Konno1,2，Tomoe Murakami 1,2，Fumie Koyama2，Hnayo_Ueoka Nakanishi2,3，Toru Hisabori1,2 1Chemical Resources Laboratory, Tokyo Institute of Technology 2ATP System Project, ERATO, JST 3Laboratory of Cell Dynamics, Graduate School of Bioagricultural Sciences, Nagoya University 1P531 Self-regulated kinesin motility. Tomonobu M. Watanabe1，Toshio Yanagida2，○Atsuko H. Iwane2 1Nanomed., Biomed. Eng. Res. Org., Tohoku Univ. 2Lab. for Nanobiology, Grad. Sch. of Frontier Biosci., Osaka Univ. 1P532 Simultaneous observation of rotatory and stepping motions of single myosin II molecules ○So Nishikawa1，Masatoshi Nishikawa1，Toshio Yanagida 1,2 1Soft Nano Machine, CREST, JST 2Frontier Biosciences, Osaka Univ. 1P533 Mechanism of Inhibition of the V-type Molecular Motor by Tributyltin Chloride ○Mizuho Takeda1，Hiromi Imamura2，Katsuya Shimabukuro3，Chiyo Ikeda4，Masasuke Yoshida1,4，Ken Yokoyama4 1Tokyo Institute of Technology 2Osaka Univ. 3Florida State Univ. 4Japan Science and Technology Agency 1P534 Loading direction controls the ADP affinity of myosin V. ○Yusuke Oguchi1，Sergey V. Mikhailenko1，Adrian O. Olivares2，Enrique M. De La Cruz2，Shin'ichi Ishiwata1 1Department of Physics, School of Science and Engineering, Waseda University 2Molecular Biophysics & Biochemistry Department, Yale University 1P535 Extracting Dynamics from Time Series Data of Single Molecule Experiments on a Photo-by-Photo Basis ○Chun Biu Li1,2，Tamiki Komatsuzaki1,2,3 1Nonlinear Science Laboratory, Kobe University 2Japan Science and Technology (JST/CREST) 3Department of Theoretical Studies, Institute for Molecular Science, Okazaki 1P536 Single molecule imaging of nerve growth factor receptor trkA expressed in the growth cones of dorsal root ganglion explants ○Tomomi Tani1，Kenta Saito1，Takeharu Nagai1 1RIES, Hokkaido Univ. 1P537 Randomness and Memory in Single Molecule Time Series ○Kazuto Sei1，Akinori Baba1,2，Chun Biu Li1,2，Tamiki Komatsuzaki1,2,3 1Nonlinear Science Laboratory, Department of Earth and Planetary Science, Faculty of Science, Kobe University, Nada Kobe 657-8501 2Japan Science and Technology Corporation (JST)/Core Research for Evolutional Science and Technology (CREST), Nada, Kobe 657-8501 3Japan and Department of Theoretical Studies, Institute for Molecular Science, Myodaiji, Okazaki 444-8585 1P538 Single-molecule imaging of Ras-PI3K signaling in chemotaxing Dictyostelium cells ○Dean Thumkeo1，Takuji Yoshihara1，Toshio Yanagida1，Masahiro Ueda1 1Laboratory of Nanobiology, Graduate School of Frontier Biosciences, Osaka University 1P539 Applicability of local ergodic state analysis of single molecule time series ○Akinori Baba1，Tamiki Komatsuzaki1,2 1Faculty of Science, Kobe Univ., JST/CREST 2Dept. of Theoretical Studies, Institute for Molecular Science 1P540 Single-molecular behavior of rhodopsin in native disc membrane ○Fumio Hayashi1 1Dept. of Biology, Kobe Univ. 1P541 Multiple domain interactions between the single cell adhesion molecule ,nectin and cadherin, revealed by high sensitive force measurements ○Yoshikazu Tsukasaki1，Kazuo Kitamura2，Kazuya Shimizu3，Atsuko_H. Iwane1，Yoshimi Takai 4，Toshio Yanagida1 1Dept. of Nanobiology, Osaka Univ. 2Dept. of Neuroscience, Osaka Univ. 3Dept. of Internal Medicine, Kobe Univ. 4Dept. of Molecular Biology and Biochemistry, Osaka Univ. 1P542 Single molecule imaging of chaperonin functions using zero-mode waveguides ○Taro Ueno1，Takashi Tanii2，Naonobu Shimamoto2，Takeo Miyake2，Hironori Sonobe2，Iwao Odomari2，Takashi Funatsu1 1Graduate School of Pharmaceutical Sciences, The University of Tokyo 2Graduate School of Science and Engineering, Waseda University 1P543 Single-molecule structural and functional analyses of nuclear pore complex Shotaro Otsuka1，Hirohide Takahashi1，○Shige H. Yoshimura1 1Graduate School of Biostudies, Kyoto University 1P544 Direct observation of unfolding-refolding dynamics of single cytochrome c proteins with milliseconds time resolution ○Kenji Okamoto1，Masayoshi Nishiyama1，Masahide Terazima1 1Dept. of Chemistry, Grad. Sch. of Science, Kyoto Univ. 1P545 Cross-talk free FCCS measurement in living cells ○Yasuo Takahashi1，Jun-ichi Nishimura1，Masataka Kinjo2，Atsushi Miyawaki3 1Olympus Corporation 2RIES, Hokkaido University 3Brain Science Institute, RIKEN 1P546 Monitoring of intracellular gene delivery and expression using Fluorescence Correlation Spectroscopy ○Akira Sasaki1，Masataka Kinjo1 1Laboratory of Molecular Cell Dynamics, Graduate school of Life science, Hokkaido University 1P547 Kinetic analysis of EGFR/Grb2 interactions using single-molecule imaging ○Miki Morimatsu1,2，Hiroaki Takagi2，Kosuke Ohta2，Toshio Yanagida2，Yasushi Sako2,3 1Kyoto university 2Osaka university 3RIKEN 1P548 Corralling of phospholipids and transmembrane proteins by "fences" and "pickets" in the cell membrane: single- molecule tracking study ○Takahiro Fujiwara1，Kokoro Iwasawa1，Ken Ritchie2，Kotono Murase1，Yasuhiro Umemura 1，Hidetoshi Yamashita1， Kenichi Suzuki1，Akihiro Kusumi1 1ICORP-JST and Inst. for Frontier Med. Sci., Kyoto Univ. 2Dept. of Physics, Purdue Univ. 1P549 Both MHC class II and its GPI-anchored form undergo hop diffusion as observed by single-molecule tracking ○Yasuhiro Umemura1，Takahiro Fujiwara1，Kenichi Suzuki1，Marija Vrljic2，Stefanie Y. Nishimura2，W. E. Moerner 2， Harden M. McConnell2，Akihiro Kusumi1 1Membrane Mechanisms Project, ICORP-JST, Inst. for Frontier Med. Sci., Kyoto Univ. 2Dept. of Chem. Stanford Univ. 1P550 Detection of transient arrest of lateral diffusion of membrane molecules in single-molecule tracking trajectories ○Kuniko Sakamoto1，Takahiro Fujiwara1，Akihiro Kusumi1 1Membrane Mechanisms Project, ICORP-JST, Kyoto Univ. 1P551 Single hydrogen bonds of DNA base pairs detected by intermolecular force microscopy ○Michio Hiroshima1,2，Makio Tokunaga1,2,3 1RCAI, RIKEN 2Natl. Inst. of Genetics 3Grad. Univ. for Adv. Studies 1P552 Snapshots of DNA untangling by a type-II topoisomerase ○Katsunori Yogo1，Kazuhiko Kinosita1 1Waseda Univ. 1P553 Direct observation of the twisting motion of a single DNA molecule caused by the intercalation of ethidium bromide ○Masahito Hayashi1，Yoshie Harada 1,2 1Tokyo Met. Inst. Med. Sci. 2CREST, JST 1P554 Elastic behaviour of RecA filaments ○Taro Nishinaka 1，Yuko Doi1，Eiji Yashima1,2 1ERATO, Japan Science and Technology Agency 2Institute for Advanced Research, Nagoya University 1P555 Irreversible work during a stretch-relax cycle of a single collapsed DNA ○Yoshihiro Murayama1，Hirofumi Wada2，Masaki Sano1 1Dept. of Physics, the Univ. of Tokyo 2Physik Dept. Tech. Univ. Munich 1P556 Novel microscopy for simultaneous single molecule measurement of DNA/protein interaction ○Hiroaki Yokota1，Yong-Woon Han1，Jean-François Allemand2，Xugang Xi3，Vincent Croquette2，David Bensimon2， Yoshie Harada 1 1Dept. Mol. Physiol., The Tokyo Metropol. Inst. Med. Sci. 2LPS, ENS 3LBPA, ENS Cachan 1P557 Peptide bond formation induces the breakage of Shine-Dalgarno interaction on the ribosome ○Sotaro Uemura1，Magdalena Dorywalska2，Tae-Hee Lee1，Harold Kim1，Joseph Puglisi2，Steven Chu1,3 1Stanford University, Department of Physics 2Stanford University, Department of Structural Biology 3Lawrence Berkeley National Laboratory 1P558 Real-time Imaging of DNA-Streptavidin Complex Formation in Solution using a High-Speed Atomic Force Microscope ○Mime Kobayashi1，Koji Sumitomo1，Keiichi Torimitsu1 1NTT Basic Research Labs., NTT Corp. 1P559 Nano-indentation of Single Protein Using Atomic Force Microscopy ○Hiroshi Sekiguchi1，Yasuhiro Mori1，Rehana Afrin1，Atsushi Ikai1 1Dept. of Life Sci., Tokyo Institute of Tech. 1P560 Observing mechanical unfolding and folding process of single molecular protein by AFM ○Takaaki Ishii 1，Atsuto Katano1，Yosh ih i ro M u rayama 1，Masaki Sano1 1Dept. of Phys, Tokyo Univ. 1P561 Forced Unfolding of OspA by Atomic Force Microscopy ○Shiga Kazuki1，Shohei Koide2，Hiroshi Sekiguchi1，Atsushi Ikai1 1Dept. of Bioscience and Biotechnology, Tokyo Institute of Technology Depart 2Department of Biochemistry and Molecular Biology The University of

27. Molecular dynamics simulation 1P562 Force-Induced Human Lysozyme with Camelid VHH HL6 Antibody Fragment : Dissociation A Molecular Dynamics study ○Yeng-Tseng Wang1，Heng-Chuan Kan1 1Southern Business Unit, National Center for High-Performance Computing 1P563 Consistent dynamic phenomena in amyloidogenic forms of transthyretin: a molecular dynamics study ○Craig A. Gough1,2，Takashi Gojobori1,2,3，Tadashi Imanishi2 1Integrated Database Group, JBIRC, JBIC, Tokyo 2Integrated Database Group, BIRC, AIST, Tokyo 3CIB-DDBJ, National Institute of Genetics, Mishima 1P564 All-Atom Molecular Dynamics Simulation of Conformational Changes in Adenylate Kinase ○Sotaro Fuchigami1，Mitsunori Ikeguchi1，Akinori Kidera1 1Intl. Grad. School of Arts and Sciences, Yokohama City Univ. 1P565 Molecular dynamics simulations of association and docking between an inhibitor and an enzyme. ○Hiroh Miyagawa1，Kunihiro Kitamura1 1Taisho Pharmaceutical Co., Ltd. 1P566 Molecular dynamics simulation of clustered DNA damage site with DNA repair enzyme MutM ○Mariko Higuchi1，Miroslav Pinak1 1Research Group for Radiation Effect Analysis, Japan Atomic Energy Agency 1P567 Molecular dynamics study of dynamical structure stability of giant hemoglobin from Oligobrachia mashikoi Ayumu Sugiyama1，Tetsunori Yamamoto1，○Hidemi Nagao1，Keigo Nishikawa1，Nobutaka Numoto2,3，Kunio Miki2,3， Yoshihiro Fukumori1 1Graduate School of Natural Science and Technology, Kanazawa University 2Graduate School of Science, Kyoto University 3RIKEN Harima 1P568 The theoretical study of sulfide-binding mechanism of the giant hemoglobin of Oligobrachia mashikoi ○Tetsunori Yamamoto1，Ayumu Sugiyama1，Keigo Nishikawa1，Hidemi Nagao1，Kimikazu Sugimori1，Nobutaka Numoto2,3， Kunio Miki2,3，Yoshirio Fukumori1 1Graduate School of Natural Science and Technology, Kanazawa University 2Graduate School of Science, Kyoto University 3RIKEN Harima 1P569 Molecular dynamics study of solvent water behavior in giant hemoglobin of Oligobrachia mashikoi. ○Keigo Nishikawa1，Ayumu Sugiyama1，Tetsunori Yamamoto1，Hidemi Nagao1，Nobutaka Numoto2,3，Kunio Miki2,3， Yoshihiro Fukumori1 1Graduate School of Natural Science and Technology, Kanazawa University 2Graduate School of Science, Kyoto University 3RIKEN Harima 1P570 Molecular dynamics simulations of lysozyme variants lacking some of the disulfide bridges: a comparison with NMR observations ○Tomoki Mizuguchi1，Okimasa Okada2，Shin-ichi Segawa1 1School of Science and Technology, Kwansei Gakuin University 2Technology and Development, Fuji Xerox Co., Ltd 1P571 A Computational Study of Monoamine Oxidase A Dynamics ○Rossen Apostolov1，Yasushige Yonezawa1，Haruki Nakamura1 1Istitute for Protein Research, Osaka University 1P572 Mechanism of Ion Permeation in Model Channel; Free Energy Surface and Dynamics of K+ Ion Transport in Anion- doped Carbon Nanotube ○Takashi Sumikama1，Shinji Saito2，Iwao Ohmine1 1Dept. of Chemistry, Nagoya Univ. 2Institute for Molecular Science 1P573 Folding simulation of the B-domain of staphylococcal protein A ○Kenichiro Nishi1，Tohru Terada1，Kentaro Shimizu1 1Graduate School of Agricultural and Life Sciences, The University of Tokyo 1P574 Mathematical modeling reveals nonlinear mechanism in cytochrome-c induced apoptosis ○Ryosuke Ishiwata1，Masaki Morioka1，Soichi Ogishima1，Hiroshi Tanaka1 1Dept. of Computational Biology, Tokyo Medical and Dental University 1P575 Potential structure changes of dynein stalk by molecular dynamics calculation ○Kazuki Fukae1，Kazuo Sutoh2，Takuo Yasunaga1 1Depatment of bioscience and bioinformatics, Graduate school of computer science and systems engineering, Kyusyu Institute of Tecnology 2Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo 1P576 Molecular dynamics analysis of amino acid polypeptide model in water in a model of simulated a part of Antifreeze protein type I ○Hiromi Taniguchi1，Takashi Nobekawa1，Yoshimichi Hagiwara 1 1Graduate student, Graduate school of Science and Technology, Kyoto Institute of Technology 2Graduate school of Science and Technology, Kyoto Institute of Technology 1P577 Efficient calculation of electrostatic interaction in biomolecular simulation revisited ○Jiro Shimada1，Takumi Washio 1，Hiroaki Fukunishi1，Toshikazu Takada 1 1NEC Fundamental and Environmental Research Laboratories 1P578 Coarse-graining approach of simulation of actin polymerization and elastic properties of filamentous actin. ○Tatsuya Yamada1，Shigeki Mitaku1 1Nagoya Univ. 1P579 Large-scale molecular dynamics simulations with the pairwise electrostatic interaction method for protein-solvent systems ○Gota Kikugawa1，Yasushige Yonezawa 2，Haruki Nakamura2，Ryutaro Himeno1 1CBU, RIKEN 2IPR, Osaka Univ. 1P580 Dynamics of cavity in c-Myb R2 sub-domain studied by high-pressure MD simulation ○Junya Toyokawa1，Sachiko Nakai1，Kazuyuki Akasaka1 1School of Biology-Oriented Science and Technology, Kinki University 1P581 Improving efficiency of conformation sampling in multicanonical molecular dynamics simulation ○Tohru Terada 1，Kentaro Shimizu1 1Graduate School of Agricultural and Life Sciences, The University of Tokyo 1P582 Explicit and GB/SA solvents: each with two different force fields in multicanonical conformational sampling of 25- residue polypeptide ○Daisuke Mitomo1，Yukihisa S Watanabe2，Narutoshi Kamiya3，Junichi Higo1 1Tokyo Univ. of Pharm. and Life Sci. 2JBIRC 3Med., Kobe Univ. 1P583 Efficient search of protein low-energy conformational space with a newly devised Wang-Landau molecular dynamics technique ○Takehiro Nagasima1，Akira R. Kinjo1,2，Takashi Mitsui3，Ken Nishikawa1,2 1CIB and DDBJ, National Institute of Genetics 2Dept. of Genetics, The Grad. Univ. for Advanced Studies (SOKENDAI) 3Fujitsu Limited 1P584 Amyloid β-peptides studied by the multicanonical-multioverlap algorithm ○Satoru G. Itoh1，Yuk o Okamot o 1 1Department of Physics, School of Science, Nagoya University 1P585 Explicit Symplectic Molecular Dynamics Simulation for Rigid-Body Molecules in the Canonical Ensemble ○Hisashi Okumura1，Satoru G. Itoh1，Yuko Okamoto 1 1Department of Physics, School of Science, Nagoya University 1P586 Protein conformational change derived from simulated coherent neutron scattering data ○Yasumasa Joti1，Akio Kitao1 1IMCB, Univ. of Tokyo 1P587 Folding free-energy landscapes of 10-residue proteins ○Daisuke Satoh1，Kentaro Shimizu2，Shugo Nakamura2，Tohru Terada2 1IML., The Univ. of Tokyo 2Graduate School of Agricultural and Life Sciences, The Univ. of Tokyo 1P588 Conformational change of proteins described by extended Go-like models ○Hiroo Kenzaki1,2，Masaki Sasai1,2 1JST-CREST 2Dept. of Computational Science and Engineering, Nagoya University 1P589 Coupling between protein conformational change and ligand binding reaction studied by multiple-basin energy landscape model ○Kei-ichi Okazaki1，Shoji Takada1 1Kobe univ. graduate school of science and technology 1P590 Investigation of The Structure-Function Relationship of Importin-β by Molecular Dynamics Simulations ○Yoshinori Hirano 1,2，Noriaki Okimoto2，Atsushi Suenaga2，Makoto Taiji2，Naoko Imamoto2，Masato Yasui1，Toshikazu Ebisuzaki2 1Keio Univ., School of Medicine 2RIKEN 1P591 A discrete wavelet time series analysis; the hierarchy of dynamics of biomolecules ○Sachiko Sato1，Akinori Baba2，Tamiki Komatsuzaki1,2,3 1Earth and Planetary Sciencies, Faculity of Science, Kobe Univ. 2Faculity of Science, Kobe Univ., JST/CREST 3Dept. of Theoretical Studies, Institute for Molecular Science 1P592 All atom molecular dynamics simulations of short peptides for De Novo protein structure prediction ○George Chikenji1 1Dept. of Computational Science and Engineering, Nagoya Univ

28. Macromolecular crystal growth 1P593 A computer-assisted supersaturation control system for the preparation of large protein crystal ○Katsuyoshi Nakazato1，Nobuhiro Sakurai1，Mituteru Hoshina1 1Dept.of Integrated Sciences in Physics and Biology, College of Humanities and Sciences, Nihon Univ. 1P594 Layer Silicates, Novel Nucleation-inducing Materials for Protein Crystallization ○Masahide Takehara1，Hiroyuki Oshikane1，Osamu Nureki1，Fujio Mizukami3，Kengo Sakaguchi2 1Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 2Department of Applied Biological Science, Faculty of Science and Technology, Science University of Tokyo 3Laboratory for Membrane Chemistry, National Institute of Advanced Industrial Science and Technology(AIST) 1P595 The protein rotational dynamics during the lysozyme pre-nucleation process ○Daisuke Takahashi1，Toshiaki Koga 2，Etsuko Nishimoto1，Shoji Yamashita1 1Institute of Biophysics, Faculty of Agriculture, Graduate School of Kyushu University 2National Institute of Advanced Industrial Science and Technology (AIST) Kyushu