DOCSLIB.ORG

Janibacter Terrae Bacteraemia: Two Faces of the Same Organism

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Janibacter Terrae Bacteraemia: Two Faces of the Same Organism P0413 Emerging infectious diseases Janibacter terrae bacteraemia: two faces of the same organism M.I. Fernández-Natal1,2*, J.A. Sáez-Nieto 3, S. Valdezate-Ramos3, J.M. Guerra-Laso 4, R.H. Rodríguez-Pollán 1, M.I. Bresme-Trigo 1, F. Soriano 5. 1Department of Clinical Microbiology, Complejo Asistencial Universitario de León; 2Institute of Biomedicine (IBIOMED), León; 3Bacterial Taxonomy Laboratory, Centro Nacional de Microbiología-Instituto de Salud Carlos III, Madrid; 4Department of Internal Medicine, Complejo Asistencial Universitario de León. 5Public Health, School of Physiotherapy, Madrid. Key words: Janibacter terrae, bacteraemia, antimicrobial susceptibility Methods: Introduction and Objectives: During a three years period (2001-2003) we isolated an organism presumptively The genus Janibacter (family Intrasporangiaceae, class Actinobacteria) consists of identified as a Janibacter sp. from eight blood cultures belonging to four patients (two aerobic, gram-positive, asporogenous, nonmotile bacteria that show a rod-coccus cycle positive blood cultures per each). during growth, and have high guanine-cytosine content. It has been mainly isolated from Phenotypic methods, including API CoryneTM V2.0 (bioMérieux), were used. different ecological niches ranging from soil, sludge, air, corals, insect gut, and spoiled In addition 16S rDNA amplification and sequencing was also performed and results were oriental melon. Only two Janibacter bacteraemias in humans has been reported, always BLAST compared with the deposited GeneBank sequences. after a single positive blood culture. Herewith we present the microbiological and clinical Antimicrobial susceptibility testing was determined by Etest on MHA with 5% blood features related to four patients from whom J. terrae was isolated from blood cultures. plates and read after 48 h. Results were interpreted following the CLSI recommendations “January”.: Romanesque painting of San Isidoro. León (Spain ) for coryneform organisms. Clinical charts of all patients were retrospectively reviewed. Results: - The organisms were identified by 16S rDNA amplification as J. terrae (99.6% similarity). All strains presented the same Table 1. Clinical and microbiology dates of four patients with Janibacter terrae bacteraemia API profile (3112004). Positive reaction was observed for hydrolysis of gelatin and hippurate, α-glucosidase and maltose assimilation. On the other hand, the organisms were assacharolytic, unable to assimilate NAG and PAC negative. No. and type of positive Underlying Antimicrobial Strain Age Sex Diagnosis Manipulation Fever Outcome blood cultures/no conditions therapy total 2977 66 M 2 aerobics/2 Chronic obstructive Acute bronchitis No Yes Amoxicillin- Cure pulmonary disease clavulanate - All strains were inhibited by the O/129 (150 mg) factor showing an inhibition diameter between 46-48 mm. - All strains were susceptible to imipenem, vancomycin, linezolid, daptomycin, ciprofloxacin, erythromycin, gentamicin, severe tetracycline and cotrimoxazole but resistant to cefotaxime, clindamycin, rifampin, and quinupristin-dalfopristin. Three 1167 66 F 2 aerobics/3 Diabetes Cholangitis No Yes Amoxicillin- Cure strains were penicillin-susceptible (MIC, 1 mg/L) while the forth had an MIC of 2 mg/L. clavulanate Tobramycin 2540 67 F 2 aerobics/3 Non-Hodgkin Multilobar Hickman Yes Ceftazidime Exitus lymphoma pneumonia catheter Amikacin Post-chemotherapy Metronidazole aplasia 1260 77 M 2 aerobics/3 Gastric Myelodysplastic No Yes Piperacillin- Exitus adenocarcinoma syndrome tazobactam with liver metastasis Conclusion: This is the first report showing J. terrae bacteraemia in humans. The organisms were isolated from four patients attended in different period of time having all of them two positive blood cultures, by which the possibility of a laboratory contamination seems unlikely. This organism had been exclusively isolated from soil but it is showing another face behaving as a probable opportunistic human pathogen. Bacterial identification could be achieved by molecular methods but also using standard and commercial biochemical tests. Antimicrobial susceptibility should be determined in all significant clinical isolates. Microbiologists must be aware of this organism which needs adequate methods for an accurate identification. Funding support: Research Project-GRS698/A/11 .
Load more

Recommended publications
  • Pentachlorophenol Degradation by Janibacter Sp., a New Actinobacterium Isolated from Saline Sediment of Arid Land
    Hindawi Publishing Corporation BioMed Research International Volume 2014, Article ID 296472, 9 pages http://dx.doi.org/10.1155/2014/296472 Research Article Pentachlorophenol Degradation by Janibacter sp., a New Actinobacterium Isolated from Saline Sediment of Arid Land Amel Khessairi,1,2 Imene Fhoula,1 Atef Jaouani,1 Yousra Turki,2 Ameur Cherif,3 Abdellatif Boudabous,1 Abdennaceur Hassen,2 and Hadda Ouzari1 1 UniversiteTunisElManar,Facult´ e´ des Sciences de Tunis (FST), LR03ES03 Laboratoire de Microorganisme et Biomolecules´ Actives, Campus Universitaire, 2092 Tunis, Tunisia 2 Laboratoire de Traitement et Recyclage des Eaux, Centre des Recherches et Technologie des Eaux (CERTE), Technopoleˆ Borj-Cedria,´ B.P. 273, 8020 Soliman, Tunisia 3 Universite´ de Manouba, Institut Superieur´ de Biotechnologie de Sidi Thabet, LR11ES31 Laboratoire de Biotechnologie et Valorization des Bio-Geo Resources, Biotechpole de Sidi Thabet, 2020 Ariana, Tunisia Correspondence should be addressed to Hadda Ouzari; [email protected] Received 1 May 2014; Accepted 17 August 2014; Published 17 September 2014 Academic Editor: George Tsiamis Copyright © 2014 Amel Khessairi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Many pentachlorophenol- (PCP-) contaminated environments are characterized by low or elevated temperatures, acidic or alkaline pH, and high salt concentrations. PCP-degrading microorganisms, adapted to grow and prosper in these environments, play an important role in the biological treatment of polluted extreme habitats. A PCP-degrading bacterium was isolated and characterized from arid and saline soil in southern Tunisia and was enriched in mineral salts medium supplemented with PCP as source of carbon and energy.
    [Show full text]
  • (ΐ2) United States Patent (ΐο) Patent No.: US 10,596,255 Β2 Clube (45) Date of Patent: *Mar
    US010596255B2 US010596255B2 (ΐ2) United States Patent (ΐο) Patent No.: US 10,596,255 Β2 Clube (45) Date of Patent: *Mar. 24, 2020 (54) SELECTIVELY ALTERING MICROBIOTA 39/0258; C07K 14/315; C07K 14/245; FOR IMMUNE MODULATION C07K 14/285; C07K 14/295; C12N 2310/20; C12N 2320/30 (71) Applicant: SNIPR Technologies Limited, London See application file for complete search history. (GB) (72) Inventor: Jasper Clube, London (GB) (56) References Cited (73) Assignee: SNIPR Technologies Limited, London U.S. PATENT DOCUMENTS (GB) 4,626,504 A 12/1986 Puhler et al. 5,633,154 A 5/1997 Schaefer et al. (*) Notice: Subject to any disclaimer, the term of this 8,241,498 Β2 8/2012 Summer et al. patent is extended or adjusted under 35 8,252,576 Β2 8/2012 Campbell et al. U.S.C. 154(b) by 0 days. 8,906,682 Β2 12/2014 June et al. 8,911,993 Β2 12/2014 June et al. This patent is subject to a terminal dis ­ 8,916,381 Β1 12/2014 June et al. 8,975,071 Β1 3/2015 June et al. claimer. 9,101,584 Β2 8/2015 June et al. 9,102,760 Β2 8/2015 June et al. (21) Appl. No.: 16/389,376 9,102,761 Β2 8/2015 June et al. 9,113,616 Β2 8/2015 MacDonald et al. (22) Filed: Apr. 19, 2019 9,328,156 Β2 5/2016 June et al. 9,464,140 Β2 10/2016 June et al. (65) Prior Publication Data 9,481,728 Β2 11/2016 June et al.
    [Show full text]
  • Diversity and Distribution of Actinobacteria Associated with Reef Coral Porites Lutea
    View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Frontiers - Publisher Connector ORIGINAL RESEARCH published: 21 October 2015 doi: 10.3389/fmicb.2015.01094 Diversity and distribution of Actinobacteria associated with reef coral Porites lutea Weiqi Kuang 1, 2 †, Jie Li 1 †, Si Zhang 1 and Lijuan Long 1* 1 CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, China, 2 College of Earth Science, University of Chinese Academy of Sciences, Beijing, China Actinobacteria is a ubiquitous major group in coral holobiont. The diversity and spatial Edited by: and temporal distribution of actinobacteria have been rarely documented. In this Sheng Qin, study, diversity of actinobacteria associated with mucus, tissue and skeleton of Porites Jiangsu Normal University, China lutea and in the surrounding seawater were examined every 3 months for 1 year on Reviewed by: Syed Gulam Dastager, Luhuitou fringing reef. The population structures of the P.lutea-associated actinobacteria National Collection of Industrial were analyzed using phylogenetic analysis of 16S rRNA gene clone libraries, which Microorganisms Resource Center, demonstrated highly diverse actinobacteria profiles in P. lutea. A total of 25 described India Wei Sun, families and 10 unnamed families were determined in the populations, and 12 genera Shanghai Jiao Tong University, China were firstly detected in corals. The Actinobacteria diversity was significantly different P. Nithyanand, SASTRA University, India between the P. lutea and the surrounding seawater. Only 10 OTUs were shared by *Correspondence: the seawater and coral samples.
    [Show full text]
  • Polycyclic Aromatic Hydrocarbon Degrading Bacteria from the Indonesian Marine Environment
    BIODIVERSITAS ISSN: 1412-033X Volume 17, Number 2, October 2016 E-ISSN: 2085-4722 Pages: 857-864 DOI: 10.13057/biodiv/d170263 Polycyclic aromatic hydrocarbon degrading bacteria from the Indonesian Marine Environment ELVI YETTI♥, AHMAD THONTOWI, YOPI Laboratory of Biocatalyst and Fermentation, Research Centre for Biotechnology, Indonesian Institute of Sciences. Cibinong Science Center, Jl. Raya Bogor Km 46 Cibinong-Bogor 16911, West Java, Indonesia. Tel. +62-21-8754587, Fax. +62-21-8754588, ♥email: [email protected] Manuscript received: 20 April 2016. Revision accepted: 20 October 2016. Abstract. Yetti E, Thontowi A, Yopi. 2016. Polyaromatic hydrocarbon degrading bacteria from the Indonesian Marine Environment. Biodiversitas 17: 857-864. Oil spills are one of the main causes of pollution in marine environments. Oil degrading bacteria play an important role for bioremediation of oil spill in environment. We collected 132 isolates of marine bacteria isolated from several Indonesia marine areas, i.e. Pari Island, Jakarta, Kamal Port, East Java and Cilacap Bay, Central Java. These isolates were screened for capability to degrade polyaromatic hydrocarbons (PAHs). Selection test were carried out qualitatively using sublimation method and growth assay of the isolates on several PAHs i.e. phenanthrene, dibenzothiophene, fluorene, naphtalene, phenotiazine, and pyrene. The fifty-eight isolates indicated in having capability to degrade PAHs, consisted of 25 isolates were positive on naphthalene (nap) and 20 isolates showed ability to grow in phenanthrene (phen) containing media. Further, 38 isolates were selected for dibenzothiophene (dbt) degradation and 25 isolates were positive on fluorene (flr). On the other hand, 23 isolates presented capability to degrade in phenothiazine (ptz) and 15 isolates could grow in media with pyrene (pyr).
    [Show full text]
  • Non-Commercial Use Only
    Infectious Disease Reports 2019; volume 11:8132 Janibacter species with most reliable diagnostic tool. However, evidence of genomic sometimes a diagnosis cannot be made Correspondence: Ying Bai, Division of using blood culture because of poor labora- Vector-Borne Diseases, Centers for Disease polymorphism isolated tory infrastructure, presence of uncommon Control and Prevention, 3156 Rampart Road, from resected heart valve pathogens, or pretreatment of the patient Fort Collins, Colorado 80521 USA. in a patient with aortic stenosis with antibiotics prior to sample collection. Tel. 1-970-266-3555. Up to 35% of all infective endocarditis E-mail: [email protected] cases remain blood culture negative either Lile Malania,1 Ying Bai,2 Key words: aortic stenosis; bacteria; genome 3 4 due to slow growth of the bacteria or inap- polymorphism; Janibacter. Kamil Khanipov, Marika Tsereteli, 2 5 1 propriate media. Mikheil Metreveli, David Tsereteli, Calcific aortic stenosis is also a major 1 1 Acknowledgements: The authors would like Ketevan Sidamonidze, Paata Imnadze, problem in many countries. In the United to thank Natalia Abazashvili, Tamriko 3 2 Yuriy Fofanov, Michael Kosoy States, the incidence rate of aortic stenosis Giorgadze, and Nazibrola Chitadze for their 1National Center for Disease Control has been reported as 27.1 per 10,000.3 The assistance with laboratory tests; Guram and Public Health, Tbilisi, Georgia; mechanism of calcification remains unclear. Katstadze, and Neli Chakvetadze for their par- ticipation in investigation of the human case; 2Division of Vector-Borne Diseases, The hypothesis is that low grade chronic or recurrent bacterial endocarditis with specif- Levent Albayrak and George Golovko for the Centers for Disease Control and assistance with genome analysis; and ic calcifiable bacteria is a cause of calcifica- Prevention, Fort Collins, CO, USA; 4 Christina Nelson for careful revision of the 3Department of Pharmacology and tion of the aortic valves.
    [Show full text]
  • WO 2014/121298 A2 7 August 2014 (07.08.2014) P O P C T
    (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (10) International Publication Number (43) International Publication Date WO 2014/121298 A2 7 August 2014 (07.08.2014) P O P C T (51) International Patent Classification: VULIC, Marin; c/o Seres Health, Inc., 161 First Street, A61K 39/02 (2006.01) Suite 1A, Cambridge, MA 02142 (US). (21) International Application Number: (74) Agents: HUBL, Susan, T. et al; Fenwick & West LLP, PCT/US2014/014738 Silicon Valley Center, 801 California Street, Mountain View, CA 94041 (US). (22) International Filing Date: 4 February 2014 (04.02.2014) (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, English (25) Filing Language: AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, (26) Publication Language: English BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, (30) Priority Data: HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, 61/760,584 4 February 2013 (04.02.2013) US KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, 61/760,585 4 February 2013 (04.02.2013) US MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, 61/760,574 4 February 2013 (04.02.2013) us OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, 61/760,606 4 February 2013 (04.02.2013) us SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, 61/926,918 13 January 2014 (13.01.2014) us TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, (71) Applicant: SERES HEALTH, INC.
    [Show full text]
  • View Full Text-PDF
    Int.J.Curr.Microbiol.App.Sci (2020) 9(4): 284-302 International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 9 Number 4 (2020) Journal homepage: http://www.ijcmas.com Original Research Article https://doi.org/10.20546/ijcmas.2020.904.035 Optimization of Nutritional Variables Using Response Surface Methodology for Enhanced Antifungal Metabolite Production by Janibacter sp. RC18 from Turmeric Rhizosphere Ruth Chiamaka Osaro-Matthew*, Francis Sopuruchukwu Ire and Nnenna Frank-peterside Department of Microbiology University of Port Harcourt Choba, Nigeria *Corresponding author ABSTRACT Antifungal metabolites from rare actinomycetes have been found attractive for application due to its novelty, potency and environmental friendliness. K e yw or ds Optimization of process variables for enhanced production of antifungal metabolite in Janibacter sp. RC18 was carried out in this study. One factor at a Janibacter sp. time (OFAT) was used for preliminary optimization of fermentation variables RC18, Antifungal (time, temperature, initial pH, carbon and nitrogen sources). A three factor central metabolite , OFAT, composite design (CCD) and response surface methodology (RSM) were Central composite design; Response employed for optimization of the selected significant nutritional variable (starch, surface soybean meal) and CaCO3. The optimum antifungal metabolite production was methodology o obtained at day 7 incubation period inhibition, temperature 30 C, pH 8, starch as carbon source and soybean meal as nitrogen source. Response surface analysis Article Info revealed that the optimum value of the variables were 10.76 g/l of starch, 11.95 g/l Accepted: of soybean meal and 1.57 g/l of CaCO . Under this optimal condition antifungal 04 March 2020 3 Available Online: metabolite production was 23.7 ± 0.09 mm which increased by 22.33 % compared 10 April 2020 to OFAT optimized medium (18.4 ± 0.06 mm).
    [Show full text]
  • Abstract Tracing Hydrocarbon
    ABSTRACT TRACING HYDROCARBON CONTAMINATION THROUGH HYPERALKALINE ENVIRONMENTS IN THE CALUMET REGION OF SOUTHEASTERN CHICAGO Kathryn Quesnell, MS Department of Geology and Environmental Geosciences Northern Illinois University, 2016 Melissa Lenczewski, Director The Calumet region of Southeastern Chicago was once known for industrialization, which left pollution as its legacy. Disposal of slag and other industrial wastes occurred in nearby wetlands in attempt to create areas suitable for future development. The waste creates an unpredictable, heterogeneous geology and a unique hyperalkaline environment. Upgradient to the field site is a former coking facility, where coke, creosote, and coal weather openly on the ground. Hydrocarbons weather into characteristic polycyclic aromatic hydrocarbons (PAHs), which can be used to create a fingerprint and correlate them to their original parent compound. This investigation identified PAHs present in the nearby surface and groundwaters through use of gas chromatography/mass spectrometry (GC/MS), as well as investigated the relationship between the alkaline environment and the organic contamination. PAH ratio analysis suggests that the organic contamination is not mobile in the groundwater, and instead originated from the air. 16S rDNA profiling suggests that some microbial communities are influenced more by pH, and some are influenced more by the hydrocarbon pollution. BIOLOG Ecoplates revealed that most communities have the ability to metabolize ring structures similar to the shape of PAHs. Analysis with bioinformatics using PICRUSt demonstrates that each community has microbes thought to be capable of hydrocarbon utilization. The field site, as well as nearby areas, are targets for habitat remediation and recreational development. In order for these remediation efforts to be successful, it is vital to understand the geochemistry, weathering, microbiology, and distribution of known contaminants.
    [Show full text]
  • Insights Into the Cultured Bacterial Fraction of Corals
    Insights into the cultured bacterial fraction of corals Michael Sweet ( [email protected] ) University of Derby https://orcid.org/0000-0003-4983-8333 Helena Villela Federal University of Rio de Janeiro Tina Keller-Costa Institute for Bioengineering and Biosciences Rodrigo Costa University of Lisbon https://orcid.org/0000-0002-5932-4101 Stefano Romano The Quadram Institute Bioscience https://orcid.org/0000-0002-7600-1953 David Bourne James Cook University Anny Cardenas University of Konstanz Megan Huggett The University of Newcastle, 10 Chittaway Rd, Ourimbah 2258 NSW Australia https://orcid.org/0000- 0002-3401-0704 Allison Kerwin McDaniel College, Westminster Felicity Kuek Pennsylvania State University Monica Medina Pennsylvania State University Julie Meyer Genetics Institute, University of Florida https://orcid.org/0000-0003-3382-3321 Moritz Müller Swinburne University of Technology Sarawak Campus https://orcid.org/0000-0001-8485-1598 Joseph Pollock Pennsylvania State University Michael Rappé University of Hawaii at Manoa Mathieu Sere University of Derby Koty Sharp Roger Williams University Christian Voolstra University of Konstanz https://orcid.org/0000-0003-4555-3795 Maren Ziegler Justus Liebig University Giessen Raquel Peixoto University of California, Davis https://orcid.org/0000-0002-9536-3132 Article Keywords: symbiosis, holobiont, metaorganism, cultured microorganisms, coral, probiotics, benecial microbes Posted Date: November 21st, 2020 DOI: https://doi.org/10.21203/rs.3.rs-105866/v1 License: This work is licensed under a Creative Commons Attribution 4.0 International License. Read Full License 1 Insights into the cultured bacterial fraction of corals 2 Sweet, Michael1*#; Villela, Helena2*; Keller-Costa, Tina3*; Costa, Rodrigo3,4*; Romano, 3 Stefano5*; Bourne, David G.6; Cárdenas, Anny7; Huggett, Megan J.8,9; Kerwin, Allison H.10; 4 Kuek, Felicity11; Medina, Mónica11; Meyer, Julie L.12; Müller, Moritz13; Pollock, F.
    [Show full text]
  • Sepsis Diagnostic Methods and Protocols M ETHODS in MOLECULAR BIOLOGY
    Methods in Molecular Biology 1237 Nicasio Mancini Editor Sepsis Diagnostic Methods and Protocols M ETHODS IN MOLECULAR BIOLOGY Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hat fi eld, Hertfordshire, AL10 9AB, UK For further volumes: http://www.springer.com/series/7651 Sepsis Diagnostic Methods and Protocols Edited by Nicasio Mancini University Vita-Salute San Raffaele, Milan, Italy Editor Nicasio Mancini University Vita-Salute San Raffaele Milan , Italy ISSN 1064-3745 ISSN 1940-6029 (electronic) ISBN 978-1-4939-1775-4 ISBN 978-1-4939-1776-1 (eBook) DOI 10.1007/978-1-4939-1776-1 Springer New York Heidelberg Dordrecht London Library of Congress Control Number: 22014949861 © Springer Science+Business Media New York 2015 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifi cally the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfi lms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. Exempted from this legal reservation are brief excerpts in connection with reviews or scholarly analysis or material supplied specifi cally for the purpose of being entered and executed on a computer system, for exclusive use by the purchaser of the work. Duplication of this publication or parts thereof is permitted only under the provisions of the Copyright Law of the Publisher’s location, in its current version, and permission for use must always be obtained from Springer.
    [Show full text]
  • Janibacter Terrae Sp. Nov., a Bacterium Isolated from Soil Around a Wastewater Treatment Plant
    International Journal of Systematic and Evolutionary Microbiology (2000), 50, 1821–1827 Printed in Great Britain Janibacter terrae sp. nov., a bacterium isolated from soil around a wastewater treatment plant Jung-Hoon Yoon,1 Keun-Chul Lee,1 Seok-Sung Kang,1,2 Yung Hee Kho,1 Kook Hee Kang2 and Yong-Ha Park1 Author for correspondence: Yong-Ha Park. Tel: j82 42 860 4620. Fax: j82 42 860 4598. e-mail: yhpark!kribb4680.kribb.re.kr 1 Korea Research Institute of A bacterial strain, CS12T, which was isolated from soil around a wastewater Bioscience and treatment plant, was subjected to a polyphasic taxonomic study using Biotechnology (KRIBB), T PO Box 115, Yusong, phenotypic characterizations and genetic methods. The cell wall of strain CS12 Taejon, Korea contains meso-diaminopimelic acid as the diamino acid but no arabinose and 2 Department of Food and galactose. The predominant menaquinone is MK-8(H4). Mycolic acids are Life Science, absent. Strain CS12T has a cellular fatty acid profile containing saturated, Sungkyunkwan University, unsaturated, branched and 10-methyl fatty acids. The major fatty acids are iso- Chunchun-dong 300, Jangan-gu, Suwon, Korea C16:0,C18:1 ω9c and anteiso-C17:0. The GMC content is 69 mol%. A phylogenetic tree based on 16S rDNA sequences showed that strain CS12T forms an evolutionary lineage within the radiation enclosing the members of the family Intrasporangiaceae and, in particular, a coherent cluster with Janibacter limosus DSM 11140T. The level of 16S rDNA similarity between strain CS12T and J. limosus DSM 11140T is 987%. The phenotypic characteristics and DNA–DNA relatedness data indicate that strain CS12T should be distinguished from J.
    [Show full text]
  • Janibacter Limosus Gen. Nov., Sp. Meso
    INTERNATIONALJOURNAL OF SYSTEMATICBACTERIOLOGY, Apr. 1997, p. 529-534 Vol. 47, No. 2 0020-7713/97/$04.00+0 Copyright 0 1997, International Union of Microbiological Societies Janibacter limosus gen. nov., sp. nov., a New Actinomycete with meso-Uiaminopimelic Acid in the Cell Wall KARIN MARTIN,l* PETER SCHUMA",2 FREDERICK A. RAINEY,3 BARBARA SCHUETZE,' AND INGRID GROTH' Hans-Knoll-Institut fur Naturstoff-Forschung e. V, and DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Aussenstelle Jena, 0-07745 Jena, and DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, 0-38124 Braunschweig, Germany New gram-positive bacteria were isolated from 1-year-old sludge from a wastewater treatment plant. The isolates are coccoid to rod-shaped, nonmotile aerobes that form neither spores nor mycelia. They are charac- terized by a peptidoglycan with directly cross-linked rneso-diaminopimelic acid (type Aly), by the presence of menaquinone MK-S(H,), and by the lack of mycolic acids. The strains have complex fatty acid patterns with i-C16:oand straight-chain saturated and unsaturated fatty acids as major components. The G+C content of the DNA is 70 mol%. The results of chemotaxonomic studies and a 16s ribosomal DNA sequence comparison support our proposal to assign these bacteria to a new genus, the genus Janibacter gen. nov.; the type species is Junibucter Zirnosus sp. nov., and the type strain of J. Zirnosus is strain HKI 83 (= DSM 11140). Both spore-forming and asporogenous actinomycetes have and starch were determined by the methods of Gledhill and Casida (10). The been screened during the last few years for useful bioactive cytochrome oxidase test was performed by using DrySlide Oxidase Test strips (Difco).
    [Show full text]