DOCSLIB.ORG

Janibacter Terrae Sp. Nov., a Bacterium Isolated from Soil Around a Wastewater Treatment Plant

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Janibacter Terrae Sp. Nov., a Bacterium Isolated from Soil Around a Wastewater Treatment Plant International Journal of Systematic and Evolutionary Microbiology (2000), 50, 1821–1827 Printed in Great Britain Janibacter terrae sp. nov., a bacterium isolated from soil around a wastewater treatment plant Jung-Hoon Yoon,1 Keun-Chul Lee,1 Seok-Sung Kang,1,2 Yung Hee Kho,1 Kook Hee Kang2 and Yong-Ha Park1 Author for correspondence: Yong-Ha Park. Tel: j82 42 860 4620. Fax: j82 42 860 4598. e-mail: yhpark!kribb4680.kribb.re.kr 1 Korea Research Institute of A bacterial strain, CS12T, which was isolated from soil around a wastewater Bioscience and treatment plant, was subjected to a polyphasic taxonomic study using Biotechnology (KRIBB), T PO Box 115, Yusong, phenotypic characterizations and genetic methods. The cell wall of strain CS12 Taejon, Korea contains meso-diaminopimelic acid as the diamino acid but no arabinose and 2 Department of Food and galactose. The predominant menaquinone is MK-8(H4). Mycolic acids are Life Science, absent. Strain CS12T has a cellular fatty acid profile containing saturated, Sungkyunkwan University, unsaturated, branched and 10-methyl fatty acids. The major fatty acids are iso- Chunchun-dong 300, Jangan-gu, Suwon, Korea C16:0,C18:1 ω9c and anteiso-C17:0. The GMC content is 69 mol%. A phylogenetic tree based on 16S rDNA sequences showed that strain CS12T forms an evolutionary lineage within the radiation enclosing the members of the family Intrasporangiaceae and, in particular, a coherent cluster with Janibacter limosus DSM 11140T. The level of 16S rDNA similarity between strain CS12T and J. limosus DSM 11140T is 987%. The phenotypic characteristics and DNA–DNA relatedness data indicate that strain CS12T should be distinguished from J. limosus DSM 11140T. Therefore, on the basis of the data presented, a new species of the genus Janibacter, Janibacter terrae, is proposed. The type strain of the new species is strain CS12T (l KCCM 80001T l JCM 10705T). Keywords: Janibacter terrae sp. nov., actinomycete, polyphasic taxonomy INTRODUCTION many strains from soil around a wastewater treatment plant. Among them, one isolate (strain CS12T)was The genus Janibacter was recently proposed by Martin considered to be a Janibacter-like strain from the et al. (1997) with one species, Janibacter limosus. This results of 16S rDNA sequence comparisons. Accord- species was isolated from a 1-year-old sludge sample ingly, strain CS12T has been subjected to a polyphasic collected from a wastewater treatment plant. The characterization to investigate the possibility that it is genus Janibacter has been placed in the family Intra- a second species of the genus Janibacter. Here, we sporangiaceae, together with the genera Intraspor- describe the morphological, phenotypic, phylogenetic angium, Terrabacter, Terracoccus and Sanguibacter and genomic characteristics of this strain. On the basis (Stackebrandt et al., 1997; Prauser et al., 1997). Since of the results described, strain CS12T is considered to there is currently only one validly described species in be a new species of the genus Janibacter, for which we the genus Janibacter, the finding of new species may be propose the name Janibacter terrae sp. nov. very meaningful from the point of view of biological diversity of this genus. Recently, we have isolated many strains that are important for the bioremediation METHODS of toxic aromatic compounds from a wastewater Bacterial strain and culture conditions. Strain CS12T was treatment plant in Korea and some of these strains isolated from a soil sample taken from around a wastewater have been found to be new actinomycete species (Cho treatment plant in Korea by dilution plating on trypticase et al., 1998; Yoon et al., 1999). We have also isolated soy agar (BBL). For investigating its morphological and physiological characteristics, strain CS12T was grown at ................................................................................................................................................. 28 mC on solid or in liquid R medium (Martin et al., 1997). The GenBank accession number for the 16S rDNA sequence of strain CS12T Cell mass for analyses of its cell wall, menaquinones, mycolic is AF176948. acids and polar lipids was obtained from growth in liquid R 01369 # 2000 IUMS 1821 J.-H. Yoon and others Terrabacter tumescens KCTC 9133T (AF005023) 83·7 T 99·5 Terracoccus luteus DSM 44267 (Y11928) Intrasporangium calvum IFO 12989T (D85486) 85·5 Strain CS12T (AF176948) 100 Janibacter limosus DSM 11140T (Y08539) Sanguibacter inulinus NCFB 3024T (X79451) 56·2 Sanguibacter keddieii NCFB 3025T (X79450) 55·8 100 Sanguibacter suarezii NCFB 3023T (X79452) Cellulomonas flavigena NCIMB 8073T (X79463) 81·8 Microbacterium lacticum DSM 20427T (X77441) Brachybacterium faecium DSM 4810T (X83810) 98·9 T 56·2 Dermabacter hominis DSM 7083 (X91034) Jonesia denitrificans DSM 20603T (X83811) Brevibacterium linens DSM 20425T (X77451) Dermatophilus congolensis ATCC 14637T (L40615) 88·1 Micrococcus luteus ATCC 381 (M38242) Nocardia asteroides DSM 43757T (X80606) 95·9 T 58·2 Rhodococcus rhodochrous DSM 43241 (X79288) Pseudonocardia halophobica DSM 43089T (Y08534) Geodermatophilus obscurus DSM 43160T (X92356) Microsphaera multipartita JCM 9543T (Y08541) Nocardioides albus KCTC 9186T (AF004988) 100 Luteococcus japonicus DSM 10546T (Z78208) Streptomyces griseus subsp. griseus KCTC 9080T (M76388) Atopobium minutum NCFB 2751T (X67148) 0·01 ................................................................................................................................................................................................................................................................................................................. Fig. 1. Phylogenetic tree showing the positions of strain CS12T and some other related taxa based on 16S rDNA sequences. Scale bar represents 0n01 substitution per nucleotide position. Bootstrap values (expressed as percentages of 1000 replications) greater than 50% are shown at the branch points. medium. Biomass of strain CS12T and J. limosus DSM agar (BBL), nutrient agar and solid R medium for fatty acid 11140T for DNA extraction was also obtained from growth methyl ester (FAME) analysis. in liquid R medium. The strains were cultivated at 28 mCon Morphological and physiological tests. The morphology of a horizontal shaker at 150 r.p.m. and the broth cultures were cells was examined by light microscopy and transmission checked for purity microscopically before they were har- electron microscopy (TEM). Presence or absence of flagella vested by centrifugation. Strain CS12T and J. limosus DSM was examined by TEM using cells from exponentially 11140T were also grown at 28 mC for 7 d on trypticase soy growing cultures. These were negatively stained with 1% 1822 International Journal of Systematic and Evolutionary Microbiology 50 Janibacter terrae sp. nov. (w\v) phosphotungstic acid, and after air drying, grids were Nucleotide sequence accession numbers. GenBank\EMBL examined with a model CM-20 transmission electron micro- accession numbers for reference 16S rDNA gene sequences scope (Philips). Catalase activity was determined by bubble used in the phylogenetic analysis are shown in Fig. 1. formation in a 3% hydrogen peroxide solution. Oxidase activity was determined by oxidation of 1% p-aminodi- methylaniline oxalate. DNase activity was determined as RESULTS described previously (Cowan & Steel, 1965) with DNase test Morphological and physiological characteristics agar (Difco). Nitrate reduction, indole production, methyl T red and Voges–Proskauer reactions, hydrogen sulfide pro- Strain CS12 is a Gram-positive, non-acid-fast, non- duction and hydrolysis of aesculin were tested as described spore-forming and non-motile bacterium. Its cells are previously (Lanyi, 1987). Hydrolyses of casein, gelatin, cocci, which are 0n6–1n1 µm in diameter after 24 h hypoxanthine, starch, Tween 80, tyrosine and xanthine, and culture in liquid R medium at 30 mC and occur singly, production of urease were also determined as described in pairs, in short chains or often in irregular clumps. previously (Cowan & Steel, 1965). Hydrolysis of arbutin was Cells were always cocci in all growth stages, both in determined according to the method of Kurup & Fink liquid and on solid R media. Colonies on R and (1975). Acid production from carbohydrates was detected nutrient agar were similar to those of J. limosus DSM by the method of Hugh & Leifson (1953). Tolerance of NaCl 11140T in colour and morphology. Colonies of strain and growth at various temperatures were measured on R T medium and brain heart infusion (BHI) medium. CS12 were cream-coloured on R agar and pale- cream-coloured on nutrient agar. Colonies of J. T Isolation of DNA. Chromosomal DNA was isolated and limosus DSM 11140 on nutrient agar were described purified according to the method of Yoon et al. (1996), with as white by Martin et al. (1997), but it is more the exception that ribonuclease T1 was used together with appropriate to describe these as being pale-cream- ribonuclease A. coloured. Colonies of strain CS12T were circular, Chemotaxonomic characterization. The isomer type of the opaque, glistening and convex on R and nutrient agar. The growth of strain CS12T on trypticase soy agar was diamino acid of the cell wall was analysed by the method of T Komagata & Suzuki (1987) and wall sugars were determined poor relative to J. limosus DSM 11140 on the same by the method of Saddler et al. (1991). Menaquinones were medium. Neither substrate mycelia nor primary my- analysed as described by
Load more

Recommended publications
  • Pentachlorophenol Degradation by Janibacter Sp., a New Actinobacterium Isolated from Saline Sediment of Arid Land
    Hindawi Publishing Corporation BioMed Research International Volume 2014, Article ID 296472, 9 pages http://dx.doi.org/10.1155/2014/296472 Research Article Pentachlorophenol Degradation by Janibacter sp., a New Actinobacterium Isolated from Saline Sediment of Arid Land Amel Khessairi,1,2 Imene Fhoula,1 Atef Jaouani,1 Yousra Turki,2 Ameur Cherif,3 Abdellatif Boudabous,1 Abdennaceur Hassen,2 and Hadda Ouzari1 1 UniversiteTunisElManar,Facult´ e´ des Sciences de Tunis (FST), LR03ES03 Laboratoire de Microorganisme et Biomolecules´ Actives, Campus Universitaire, 2092 Tunis, Tunisia 2 Laboratoire de Traitement et Recyclage des Eaux, Centre des Recherches et Technologie des Eaux (CERTE), Technopoleˆ Borj-Cedria,´ B.P. 273, 8020 Soliman, Tunisia 3 Universite´ de Manouba, Institut Superieur´ de Biotechnologie de Sidi Thabet, LR11ES31 Laboratoire de Biotechnologie et Valorization des Bio-Geo Resources, Biotechpole de Sidi Thabet, 2020 Ariana, Tunisia Correspondence should be addressed to Hadda Ouzari; [email protected] Received 1 May 2014; Accepted 17 August 2014; Published 17 September 2014 Academic Editor: George Tsiamis Copyright © 2014 Amel Khessairi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Many pentachlorophenol- (PCP-) contaminated environments are characterized by low or elevated temperatures, acidic or alkaline pH, and high salt concentrations. PCP-degrading microorganisms, adapted to grow and prosper in these environments, play an important role in the biological treatment of polluted extreme habitats. A PCP-degrading bacterium was isolated and characterized from arid and saline soil in southern Tunisia and was enriched in mineral salts medium supplemented with PCP as source of carbon and energy.
    [Show full text]
  • (ΐ2) United States Patent (ΐο) Patent No.: US 10,596,255 Β2 Clube (45) Date of Patent: *Mar
    US010596255B2 US010596255B2 (ΐ2) United States Patent (ΐο) Patent No.: US 10,596,255 Β2 Clube (45) Date of Patent: *Mar. 24, 2020 (54) SELECTIVELY ALTERING MICROBIOTA 39/0258; C07K 14/315; C07K 14/245; FOR IMMUNE MODULATION C07K 14/285; C07K 14/295; C12N 2310/20; C12N 2320/30 (71) Applicant: SNIPR Technologies Limited, London See application file for complete search history. (GB) (72) Inventor: Jasper Clube, London (GB) (56) References Cited (73) Assignee: SNIPR Technologies Limited, London U.S. PATENT DOCUMENTS (GB) 4,626,504 A 12/1986 Puhler et al. 5,633,154 A 5/1997 Schaefer et al. (*) Notice: Subject to any disclaimer, the term of this 8,241,498 Β2 8/2012 Summer et al. patent is extended or adjusted under 35 8,252,576 Β2 8/2012 Campbell et al. U.S.C. 154(b) by 0 days. 8,906,682 Β2 12/2014 June et al. 8,911,993 Β2 12/2014 June et al. This patent is subject to a terminal dis ­ 8,916,381 Β1 12/2014 June et al. 8,975,071 Β1 3/2015 June et al. claimer. 9,101,584 Β2 8/2015 June et al. 9,102,760 Β2 8/2015 June et al. (21) Appl. No.: 16/389,376 9,102,761 Β2 8/2015 June et al. 9,113,616 Β2 8/2015 MacDonald et al. (22) Filed: Apr. 19, 2019 9,328,156 Β2 5/2016 June et al. 9,464,140 Β2 10/2016 June et al. (65) Prior Publication Data 9,481,728 Β2 11/2016 June et al.
    [Show full text]
  • Diversity and Distribution of Actinobacteria Associated with Reef Coral Porites Lutea
    View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Frontiers - Publisher Connector ORIGINAL RESEARCH published: 21 October 2015 doi: 10.3389/fmicb.2015.01094 Diversity and distribution of Actinobacteria associated with reef coral Porites lutea Weiqi Kuang 1, 2 †, Jie Li 1 †, Si Zhang 1 and Lijuan Long 1* 1 CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, China, 2 College of Earth Science, University of Chinese Academy of Sciences, Beijing, China Actinobacteria is a ubiquitous major group in coral holobiont. The diversity and spatial Edited by: and temporal distribution of actinobacteria have been rarely documented. In this Sheng Qin, study, diversity of actinobacteria associated with mucus, tissue and skeleton of Porites Jiangsu Normal University, China lutea and in the surrounding seawater were examined every 3 months for 1 year on Reviewed by: Syed Gulam Dastager, Luhuitou fringing reef. The population structures of the P.lutea-associated actinobacteria National Collection of Industrial were analyzed using phylogenetic analysis of 16S rRNA gene clone libraries, which Microorganisms Resource Center, demonstrated highly diverse actinobacteria profiles in P. lutea. A total of 25 described India Wei Sun, families and 10 unnamed families were determined in the populations, and 12 genera Shanghai Jiao Tong University, China were firstly detected in corals. The Actinobacteria diversity was significantly different P. Nithyanand, SASTRA University, India between the P. lutea and the surrounding seawater. Only 10 OTUs were shared by *Correspondence: the seawater and coral samples.
    [Show full text]
  • Polycyclic Aromatic Hydrocarbon Degrading Bacteria from the Indonesian Marine Environment
    BIODIVERSITAS ISSN: 1412-033X Volume 17, Number 2, October 2016 E-ISSN: 2085-4722 Pages: 857-864 DOI: 10.13057/biodiv/d170263 Polycyclic aromatic hydrocarbon degrading bacteria from the Indonesian Marine Environment ELVI YETTI♥, AHMAD THONTOWI, YOPI Laboratory of Biocatalyst and Fermentation, Research Centre for Biotechnology, Indonesian Institute of Sciences. Cibinong Science Center, Jl. Raya Bogor Km 46 Cibinong-Bogor 16911, West Java, Indonesia. Tel. +62-21-8754587, Fax. +62-21-8754588, ♥email: [email protected] Manuscript received: 20 April 2016. Revision accepted: 20 October 2016. Abstract. Yetti E, Thontowi A, Yopi. 2016. Polyaromatic hydrocarbon degrading bacteria from the Indonesian Marine Environment. Biodiversitas 17: 857-864. Oil spills are one of the main causes of pollution in marine environments. Oil degrading bacteria play an important role for bioremediation of oil spill in environment. We collected 132 isolates of marine bacteria isolated from several Indonesia marine areas, i.e. Pari Island, Jakarta, Kamal Port, East Java and Cilacap Bay, Central Java. These isolates were screened for capability to degrade polyaromatic hydrocarbons (PAHs). Selection test were carried out qualitatively using sublimation method and growth assay of the isolates on several PAHs i.e. phenanthrene, dibenzothiophene, fluorene, naphtalene, phenotiazine, and pyrene. The fifty-eight isolates indicated in having capability to degrade PAHs, consisted of 25 isolates were positive on naphthalene (nap) and 20 isolates showed ability to grow in phenanthrene (phen) containing media. Further, 38 isolates were selected for dibenzothiophene (dbt) degradation and 25 isolates were positive on fluorene (flr). On the other hand, 23 isolates presented capability to degrade in phenothiazine (ptz) and 15 isolates could grow in media with pyrene (pyr).
    [Show full text]
  • Non-Commercial Use Only
    Infectious Disease Reports 2019; volume 11:8132 Janibacter species with most reliable diagnostic tool. However, evidence of genomic sometimes a diagnosis cannot be made Correspondence: Ying Bai, Division of using blood culture because of poor labora- Vector-Borne Diseases, Centers for Disease polymorphism isolated tory infrastructure, presence of uncommon Control and Prevention, 3156 Rampart Road, from resected heart valve pathogens, or pretreatment of the patient Fort Collins, Colorado 80521 USA. in a patient with aortic stenosis with antibiotics prior to sample collection. Tel. 1-970-266-3555. Up to 35% of all infective endocarditis E-mail: [email protected] cases remain blood culture negative either Lile Malania,1 Ying Bai,2 Key words: aortic stenosis; bacteria; genome 3 4 due to slow growth of the bacteria or inap- polymorphism; Janibacter. Kamil Khanipov, Marika Tsereteli, 2 5 1 propriate media. Mikheil Metreveli, David Tsereteli, Calcific aortic stenosis is also a major 1 1 Acknowledgements: The authors would like Ketevan Sidamonidze, Paata Imnadze, problem in many countries. In the United to thank Natalia Abazashvili, Tamriko 3 2 Yuriy Fofanov, Michael Kosoy States, the incidence rate of aortic stenosis Giorgadze, and Nazibrola Chitadze for their 1National Center for Disease Control has been reported as 27.1 per 10,000.3 The assistance with laboratory tests; Guram and Public Health, Tbilisi, Georgia; mechanism of calcification remains unclear. Katstadze, and Neli Chakvetadze for their par- ticipation in investigation of the human case; 2Division of Vector-Borne Diseases, The hypothesis is that low grade chronic or recurrent bacterial endocarditis with specif- Levent Albayrak and George Golovko for the Centers for Disease Control and assistance with genome analysis; and ic calcifiable bacteria is a cause of calcifica- Prevention, Fort Collins, CO, USA; 4 Christina Nelson for careful revision of the 3Department of Pharmacology and tion of the aortic valves.
    [Show full text]
  • WO 2014/121298 A2 7 August 2014 (07.08.2014) P O P C T
    (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (10) International Publication Number (43) International Publication Date WO 2014/121298 A2 7 August 2014 (07.08.2014) P O P C T (51) International Patent Classification: VULIC, Marin; c/o Seres Health, Inc., 161 First Street, A61K 39/02 (2006.01) Suite 1A, Cambridge, MA 02142 (US). (21) International Application Number: (74) Agents: HUBL, Susan, T. et al; Fenwick & West LLP, PCT/US2014/014738 Silicon Valley Center, 801 California Street, Mountain View, CA 94041 (US). (22) International Filing Date: 4 February 2014 (04.02.2014) (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, English (25) Filing Language: AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, (26) Publication Language: English BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, (30) Priority Data: HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, 61/760,584 4 February 2013 (04.02.2013) US KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, 61/760,585 4 February 2013 (04.02.2013) US MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, 61/760,574 4 February 2013 (04.02.2013) us OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, 61/760,606 4 February 2013 (04.02.2013) us SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, 61/926,918 13 January 2014 (13.01.2014) us TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, (71) Applicant: SERES HEALTH, INC.
    [Show full text]
  • View Full Text-PDF
    Int.J.Curr.Microbiol.App.Sci (2020) 9(4): 284-302 International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 9 Number 4 (2020) Journal homepage: http://www.ijcmas.com Original Research Article https://doi.org/10.20546/ijcmas.2020.904.035 Optimization of Nutritional Variables Using Response Surface Methodology for Enhanced Antifungal Metabolite Production by Janibacter sp. RC18 from Turmeric Rhizosphere Ruth Chiamaka Osaro-Matthew*, Francis Sopuruchukwu Ire and Nnenna Frank-peterside Department of Microbiology University of Port Harcourt Choba, Nigeria *Corresponding author ABSTRACT Antifungal metabolites from rare actinomycetes have been found attractive for application due to its novelty, potency and environmental friendliness. K e yw or ds Optimization of process variables for enhanced production of antifungal metabolite in Janibacter sp. RC18 was carried out in this study. One factor at a Janibacter sp. time (OFAT) was used for preliminary optimization of fermentation variables RC18, Antifungal (time, temperature, initial pH, carbon and nitrogen sources). A three factor central metabolite , OFAT, composite design (CCD) and response surface methodology (RSM) were Central composite design; Response employed for optimization of the selected significant nutritional variable (starch, surface soybean meal) and CaCO3. The optimum antifungal metabolite production was methodology o obtained at day 7 incubation period inhibition, temperature 30 C, pH 8, starch as carbon source and soybean meal as nitrogen source. Response surface analysis Article Info revealed that the optimum value of the variables were 10.76 g/l of starch, 11.95 g/l Accepted: of soybean meal and 1.57 g/l of CaCO . Under this optimal condition antifungal 04 March 2020 3 Available Online: metabolite production was 23.7 ± 0.09 mm which increased by 22.33 % compared 10 April 2020 to OFAT optimized medium (18.4 ± 0.06 mm).
    [Show full text]
  • Abstract Tracing Hydrocarbon
    ABSTRACT TRACING HYDROCARBON CONTAMINATION THROUGH HYPERALKALINE ENVIRONMENTS IN THE CALUMET REGION OF SOUTHEASTERN CHICAGO Kathryn Quesnell, MS Department of Geology and Environmental Geosciences Northern Illinois University, 2016 Melissa Lenczewski, Director The Calumet region of Southeastern Chicago was once known for industrialization, which left pollution as its legacy. Disposal of slag and other industrial wastes occurred in nearby wetlands in attempt to create areas suitable for future development. The waste creates an unpredictable, heterogeneous geology and a unique hyperalkaline environment. Upgradient to the field site is a former coking facility, where coke, creosote, and coal weather openly on the ground. Hydrocarbons weather into characteristic polycyclic aromatic hydrocarbons (PAHs), which can be used to create a fingerprint and correlate them to their original parent compound. This investigation identified PAHs present in the nearby surface and groundwaters through use of gas chromatography/mass spectrometry (GC/MS), as well as investigated the relationship between the alkaline environment and the organic contamination. PAH ratio analysis suggests that the organic contamination is not mobile in the groundwater, and instead originated from the air. 16S rDNA profiling suggests that some microbial communities are influenced more by pH, and some are influenced more by the hydrocarbon pollution. BIOLOG Ecoplates revealed that most communities have the ability to metabolize ring structures similar to the shape of PAHs. Analysis with bioinformatics using PICRUSt demonstrates that each community has microbes thought to be capable of hydrocarbon utilization. The field site, as well as nearby areas, are targets for habitat remediation and recreational development. In order for these remediation efforts to be successful, it is vital to understand the geochemistry, weathering, microbiology, and distribution of known contaminants.
    [Show full text]
  • Insights Into the Cultured Bacterial Fraction of Corals
    Insights into the cultured bacterial fraction of corals Michael Sweet ( [email protected] ) University of Derby https://orcid.org/0000-0003-4983-8333 Helena Villela Federal University of Rio de Janeiro Tina Keller-Costa Institute for Bioengineering and Biosciences Rodrigo Costa University of Lisbon https://orcid.org/0000-0002-5932-4101 Stefano Romano The Quadram Institute Bioscience https://orcid.org/0000-0002-7600-1953 David Bourne James Cook University Anny Cardenas University of Konstanz Megan Huggett The University of Newcastle, 10 Chittaway Rd, Ourimbah 2258 NSW Australia https://orcid.org/0000- 0002-3401-0704 Allison Kerwin McDaniel College, Westminster Felicity Kuek Pennsylvania State University Monica Medina Pennsylvania State University Julie Meyer Genetics Institute, University of Florida https://orcid.org/0000-0003-3382-3321 Moritz Müller Swinburne University of Technology Sarawak Campus https://orcid.org/0000-0001-8485-1598 Joseph Pollock Pennsylvania State University Michael Rappé University of Hawaii at Manoa Mathieu Sere University of Derby Koty Sharp Roger Williams University Christian Voolstra University of Konstanz https://orcid.org/0000-0003-4555-3795 Maren Ziegler Justus Liebig University Giessen Raquel Peixoto University of California, Davis https://orcid.org/0000-0002-9536-3132 Article Keywords: symbiosis, holobiont, metaorganism, cultured microorganisms, coral, probiotics, benecial microbes Posted Date: November 21st, 2020 DOI: https://doi.org/10.21203/rs.3.rs-105866/v1 License: This work is licensed under a Creative Commons Attribution 4.0 International License. Read Full License 1 Insights into the cultured bacterial fraction of corals 2 Sweet, Michael1*#; Villela, Helena2*; Keller-Costa, Tina3*; Costa, Rodrigo3,4*; Romano, 3 Stefano5*; Bourne, David G.6; Cárdenas, Anny7; Huggett, Megan J.8,9; Kerwin, Allison H.10; 4 Kuek, Felicity11; Medina, Mónica11; Meyer, Julie L.12; Müller, Moritz13; Pollock, F.
    [Show full text]
  • Sepsis Diagnostic Methods and Protocols M ETHODS in MOLECULAR BIOLOGY
    Methods in Molecular Biology 1237 Nicasio Mancini Editor Sepsis Diagnostic Methods and Protocols M ETHODS IN MOLECULAR BIOLOGY Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hat fi eld, Hertfordshire, AL10 9AB, UK For further volumes: http://www.springer.com/series/7651 Sepsis Diagnostic Methods and Protocols Edited by Nicasio Mancini University Vita-Salute San Raffaele, Milan, Italy Editor Nicasio Mancini University Vita-Salute San Raffaele Milan , Italy ISSN 1064-3745 ISSN 1940-6029 (electronic) ISBN 978-1-4939-1775-4 ISBN 978-1-4939-1776-1 (eBook) DOI 10.1007/978-1-4939-1776-1 Springer New York Heidelberg Dordrecht London Library of Congress Control Number: 22014949861 © Springer Science+Business Media New York 2015 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifi cally the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfi lms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. Exempted from this legal reservation are brief excerpts in connection with reviews or scholarly analysis or material supplied specifi cally for the purpose of being entered and executed on a computer system, for exclusive use by the purchaser of the work. Duplication of this publication or parts thereof is permitted only under the provisions of the Copyright Law of the Publisher’s location, in its current version, and permission for use must always be obtained from Springer.
    [Show full text]
  • Janibacter Limosus Gen. Nov., Sp. Meso
    INTERNATIONALJOURNAL OF SYSTEMATICBACTERIOLOGY, Apr. 1997, p. 529-534 Vol. 47, No. 2 0020-7713/97/$04.00+0 Copyright 0 1997, International Union of Microbiological Societies Janibacter limosus gen. nov., sp. nov., a New Actinomycete with meso-Uiaminopimelic Acid in the Cell Wall KARIN MARTIN,l* PETER SCHUMA",2 FREDERICK A. RAINEY,3 BARBARA SCHUETZE,' AND INGRID GROTH' Hans-Knoll-Institut fur Naturstoff-Forschung e. V, and DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Aussenstelle Jena, 0-07745 Jena, and DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, 0-38124 Braunschweig, Germany New gram-positive bacteria were isolated from 1-year-old sludge from a wastewater treatment plant. The isolates are coccoid to rod-shaped, nonmotile aerobes that form neither spores nor mycelia. They are charac- terized by a peptidoglycan with directly cross-linked rneso-diaminopimelic acid (type Aly), by the presence of menaquinone MK-S(H,), and by the lack of mycolic acids. The strains have complex fatty acid patterns with i-C16:oand straight-chain saturated and unsaturated fatty acids as major components. The G+C content of the DNA is 70 mol%. The results of chemotaxonomic studies and a 16s ribosomal DNA sequence comparison support our proposal to assign these bacteria to a new genus, the genus Janibacter gen. nov.; the type species is Junibucter Zirnosus sp. nov., and the type strain of J. Zirnosus is strain HKI 83 (= DSM 11140). Both spore-forming and asporogenous actinomycetes have and starch were determined by the methods of Gledhill and Casida (10). The been screened during the last few years for useful bioactive cytochrome oxidase test was performed by using DrySlide Oxidase Test strips (Difco).
    [Show full text]
  • Diversity, Ecological Distribution and Biotechnological Potential of Actinobacteria Inhabiting Seamounts and Non-Seamounts in the Tyrrhenian Sea
    Diversity, ecological distribution and biotechnological potential of Actinobacteria inhabiting seamounts and non-seamounts in the Tyrrhenian Sea Item Type Article Authors Ettoumi, Besma; Chouchane, Habib; Guesmi, Amel; Mahjoubi, Mouna; Brusetti, Lorenzo; Neifar, Mohamed; Borin, Sara; Daffonchio, Daniele; Cherif, Ameur Citation Diversity, ecological distribution and biotechnological potential of Actinobacteria inhabiting seamounts and non-seamounts in the Tyrrhenian Sea 2016 Microbiological Research Eprint version Post-print DOI 10.1016/j.micres.2016.03.006 Publisher Elsevier BV Journal Microbiological Research Rights NOTICE: this is the author’s version of a work that was accepted for publication in Microbiological Research. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Microbiological Research, 1 April 2016. DOI: 10.1016/ j.micres.2016.03.006 Download date 26/09/2021 00:32:33 Link to Item http://hdl.handle.net/10754/604744 Accepted Manuscript Title: Diversity, ecological distribution and biotechnological potential of Actinobacteria inhabiting seamounts and non-seamounts in the Tyrrhenian Sea Author: Besma Ettoumi Habib Chouchane Amel Guesmi Mouna Mahjoubi Lorenzo Brusetti Mohamed Neifar Sara Borin Daniele Daffonchio Ameur Cherif PII: S0944-5013(16)30081-7 DOI: http://dx.doi.org/doi:10.1016/j.micres.2016.03.006
    [Show full text]