Sepsis Diagnostic Methods and Protocols M ETHODS in MOLECULAR BIOLOGY
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Pentachlorophenol Degradation by Janibacter Sp., a New Actinobacterium Isolated from Saline Sediment of Arid Land
Hindawi Publishing Corporation BioMed Research International Volume 2014, Article ID 296472, 9 pages http://dx.doi.org/10.1155/2014/296472 Research Article Pentachlorophenol Degradation by Janibacter sp., a New Actinobacterium Isolated from Saline Sediment of Arid Land Amel Khessairi,1,2 Imene Fhoula,1 Atef Jaouani,1 Yousra Turki,2 Ameur Cherif,3 Abdellatif Boudabous,1 Abdennaceur Hassen,2 and Hadda Ouzari1 1 UniversiteTunisElManar,Facult´ e´ des Sciences de Tunis (FST), LR03ES03 Laboratoire de Microorganisme et Biomolecules´ Actives, Campus Universitaire, 2092 Tunis, Tunisia 2 Laboratoire de Traitement et Recyclage des Eaux, Centre des Recherches et Technologie des Eaux (CERTE), Technopoleˆ Borj-Cedria,´ B.P. 273, 8020 Soliman, Tunisia 3 Universite´ de Manouba, Institut Superieur´ de Biotechnologie de Sidi Thabet, LR11ES31 Laboratoire de Biotechnologie et Valorization des Bio-Geo Resources, Biotechpole de Sidi Thabet, 2020 Ariana, Tunisia Correspondence should be addressed to Hadda Ouzari; [email protected] Received 1 May 2014; Accepted 17 August 2014; Published 17 September 2014 Academic Editor: George Tsiamis Copyright © 2014 Amel Khessairi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Many pentachlorophenol- (PCP-) contaminated environments are characterized by low or elevated temperatures, acidic or alkaline pH, and high salt concentrations. PCP-degrading microorganisms, adapted to grow and prosper in these environments, play an important role in the biological treatment of polluted extreme habitats. A PCP-degrading bacterium was isolated and characterized from arid and saline soil in southern Tunisia and was enriched in mineral salts medium supplemented with PCP as source of carbon and energy. -
Bordetella Petrii Clinical Isolate Isolates of This Species Have Been Previously Reported from 4
routine laboratory protocols. Initial susceptibility testing Bordetella petrii using disk diffusion indicated apparent susceptibility of the isolate to erythromycin, gentamicin, ceftriaxone, and Clinical Isolate piperacillin/tazobactam. The isolate was resistant to amox- icillin, co-amoxiclav, tetracycline, clindamycin, ciproflo- Norman K. Fry,* John Duncan,* Henry Malnick,* xacin, and metronidazole. After initial sensitivity results, a Marina Warner,* Andrew J. Smith,† 6-week course of oral clarithromycin (500 mg, 8 hourly) Margaret S. Jackson,† and Ashraf Ayoub† was begun. We describe the first clinical isolate of Bordetella petrii At follow-up appointments 3 months and 6 months from a patient with mandibular osteomyelitis. The only pre- after antimicrobial drug therapy ceased, clinical and radi- viously documented isolation of B. petrii occurred after the ographic findings were not unusual, and the infected area initial culture of a single strain from an environmental healed successfully. Despite the successful clinical out- source. come, the isolate was subsequently shown to be resistant to clarithromycin in vitro (Table). Improvement of the 67-year-old man visited an emergency dental clinic, osteomyelitis may also have been facilitated by the biopsy Awhere he complained of toothache in the lower right procedure, during which a sequestrum of bone was mandibular quadrant. Examination showed a root-filled removed. lower right canine tooth that was mobile and tender to per- The gram-negative bacillus (designated strain cussion. The tooth was extracted uneventfully under local GDH030510) was submitted to the Health Protection anesthesia. The patient returned after several days with Agency, Centre for Infections, London, for identification. pain at the extraction site. A localized alveolar osteitis was Preliminary tests results were consistent with those diagnosed, and local debridement measures were institut- described for members of the genus Bordetella. -
Proposal for Assessment of New Health Technologies
v4.0 11.12.2017 Proposal for assessment of new health technologies Important information – read this first! Submitted proposals for national health technologies (HTAs) will be published in full. If the proposer thinks there is information necessary for filling out the form, that should not be made public, please contact the secretariat (Nye Metoder) before submission. The proposer is aware that the form will be published in its entirety (tick): ☒ Proposer has filled out point 19 below «Interests and, if any, conflicts of interest» (tick): ☒ This form serves the purpose to submit proposals for health technology assessment (HTA) at the national level in Nye Metoder - the national system for managed introduction of new health technologies within the specialist health service in Norway. The form does not apply to proposals for research projects. A health technology assessment is a type of evidence review, and for this to be possible, documentation is required, e.g. from completed clinical trials. Lack of documentation may be one of the reasons why the Commissioning Forum (Bestillerforum RHF) does not assign a health technology assessment. If the proposal concerns a medical device, the proposer is familiar with the document «Guidance criteria for management of medical devices in the National System for Managed Introduction of New Health Technologies within the Specialist Health Service in Norway» (link) (tick): ☒ Contact information: Name of the proposer (organization / institution / company / manufacturer): ViroGates A/S, Denmark Name of proposal contact: Jesper Eugen-Olsen, PhD Telephone number: +45 51 51 80 51 E-mail address: [email protected] Date and locality: 03-01-2019, Copenhagen Side 1 av 9 v4.0 11.12.2017 1. -
Virogates Has Been Granted a New Patent to Measure the Risk of Developing Life Style Related Diseases Using Its Suparnostic ® Biomarker Product
ViroGates has been granted a new patent to measure the risk of developing life style related diseases using its suPARnostic ® biomarker product Denmark, August 22, 2011 ViroGates, that develops and distributes the biomarker suPARnostic ®, today announced that it has been granted a patent in the UK, patent number GB2461410, based on the international patent application PCT/EP2007/064497. The patent is entitled “Method and tool for predicting cancer and other diseases”. The patent grant is the first related to the international patent application, and will allow ViroGates to pursue measuring and monitoring of patients’ blood, to determine whether the individual is at risk of developing life style related diseases such as cardiovascular diseases, type-2 diabetes (T2D), COPD and cancer. The grant of this patent further strengthens ViroGates’ patent portfolio, which comprises patents related to measuring and monitoring of various infectious diseases such as HIV, tuberculosis and various conditions of sepsis. The MONICA10 study (published in the Journal of Internal Medicine, 28 May 2010 by Jesper Eugen-Olsen, Ove Andersen and Steen B. Haugaard, Hvidovre University Hospital) has been the scientific basis for establishing this relation between suPAR and life style related diseases. The study documented that suPAR was predictive of development of cardiovascular diseases, diabetes and cancer, and particularly in predicting which individuals would progress to develop lung cancer. ViroGates, supported by a network of international key opinion leaders, is working towards establishing suPARnostic ® as a clinical decision-making product worldwide. “We are very excited to receive the news of this first grant of a patent relating to predicting serious life style related diseases” comments Jakob Knudsen, CEO of ViroGates. -
Novel Biomarkers in the Management of HPV-Positive & -Negative Oropharyngeal Carcinoma January 2016
THE UNIVERSITY OF SHEFFIELD Novel Biomarkers in the Management of HPV-Positive & -Negative Oropharyngeal Carcinoma Thesis submitted to the University of Sheffield for the degree of Doctor of Philosophy Robert Bolt Department of Oral & Maxillofacial Pathology January 2016 Abstract Human Papillomavirus (HPV)-related oropharyngeal carcinoma is considered to be in the early stages of an epidemic1-12. A marked rise in the incidence of this sexually-transmissible cancer has captured the public interest, and much debate exists over both the prophylactic and therapeutic strategies currently employed to manage this healthcare priority. HPV-positive oropharyngeal carcinoma is associated with highly favourable oncological outcomes. Clinical attention over recent years has been paid to the potential de-escalation of therapy in order to account for the disease’s favourable prognosis, in addition to reducing therapeutic burden in a well-prognosticating, younger patient cohort, for which consequences of radical chemo-radiotherapy strategies may disproportionately impact on longer-term quality of life. Whilst optimising the management of the ever-increasing proportion of HPV-positive oropharyngeal carcinomas is desirable and highly justifiable, it appears the poorer prognosticating HPV-negative oropharyngeal carcinoma has at least in part become overlooked. Oropharyngeal carcinoma is unique in comparison to many other established HPV-related cancers inasmuch as a clear HPV-negative subset exists, to which established aetiological factors (tobacco smoking and alcohol consumption) strongly correlate. For most other HPV- related carcinomas, such as cervical, anal and penile, tumours classified as HPV-negative are either regarded as potentially-virus containing, or else cannot be correlated to a definitive aetiological agent. Comparison of HPV-positive and -negative oropharyngeal carcinoma therefore offers unprecedented insight into the biological significance of each aetiological agent, and how prognostication of each disease may relate to tumour behaviour at a molecular level. -
Identification of Bordetella Spp. in Respiratory Specimens From
504 Clinical Microbiology and Infection, Volume 14 Number 5, May 2008 isolates of extended-spectrum-beta-lactamase-producing Original Submission: 27 October 2007; Revised Sub- Shigella sonnei. Ann Trop Med Parasitol 2007; 101: 511–517. mission: 5 December 2007; Accepted: 19 December 21. Rice LB. Controlling antibiotic resistance in the ICU: 2007 different bacteria, different strategies. Cleve Clin J Med 2003; 70: 793–800. Clin Microbiol Infect 2008; 14: 504–506 22. Boyd DA, Tyler S, Christianson S et al. Complete nucleo- 10.1111/j.1469-0691.2008.01968.x tide sequence of a 92-kilobase plasmid harbouring the CTX-M-15 extended spectrum b-lactamase involved in an outbreak in long-term-care facilities in Toronto, Canada. Cystic fibrosis (CF) is an autosomal recessive Antimicrob Agents Chemother 2004; 48: 3758–3764. disease, characterised by defective chloride ion channels that result in multi-organ dysfunction, most notably affecting the respiratory tract. The RESEARCH NOTE alteration in the pulmonary environment is asso- ciated with increased susceptibility to bacterial infection. Recent advances in bacterial taxonomy and improved microbial identification systems Identification of Bordetella spp. in have led to an increasing recognition of the respiratory specimens from individuals diversity of bacterial species involved in CF lung with cystic fibrosis infection. Many such species are opportunistic T. Spilker, A. A. Liwienski and J. J. LiPuma human pathogens, some of which are rarely found in other human infections [1]. Processing Department of Pediatrics and Communicable of CF respiratory cultures therefore employs Diseases, University of Michigan Medical selective media and focuses on detection of School, Ann Arbor, MI, USA uncommon human pathogens. -
Iron Transport Strategies of the Genus Burkholderia
Zurich Open Repository and Archive University of Zurich Main Library Strickhofstrasse 39 CH-8057 Zurich www.zora.uzh.ch Year: 2015 Iron transport strategies of the genus Burkholderia Mathew, Anugraha Posted at the Zurich Open Repository and Archive, University of Zurich ZORA URL: https://doi.org/10.5167/uzh-113412 Dissertation Published Version Originally published at: Mathew, Anugraha. Iron transport strategies of the genus Burkholderia. 2015, University of Zurich, Faculty of Science. Iron transport strategies of the genus Burkholderia Dissertation zur Erlangung der naturwissenschaftlichen Doktorwürde (Dr. sc. nat.) vorgelegt der Mathematisch-naturwissenschaftlichen Fakultät der Universität Zürich von Anugraha Mathew aus Indien Promotionskomitee Prof. Dr. Leo Eberl (Vorsitz) Prof. Dr. Jakob Pernthaler Dr. Aurelien carlier Zürich, 2015 2 Table of Contents Summary .............................................................................................................. 7 Zusammenfassung ................................................................................................ 9 Abbreviations ..................................................................................................... 11 Chapter 1: Introduction ....................................................................................... 14 1.1.Role and properties of iron in bacteria ...................................................................... 14 1.2.Iron transport mechanisms in bacteria ..................................................................... -
See Virogates Presentation Here
ViroGates ViroGates A/S Västra Hamnen Investor Day 2 December 2020 ViroGates 2 ViroGates - a commercial stage company with approved products for the hospital sector ViroGates suPARnostic® products suPAR is the biomarker detected by ViroGates’ three CE-IVD marked suPARnostic® products and is a protein found in plasma. Description ViroGates A/S is an international medical technology ViroGates has established clinical utility of suPARnostic® supported by company developing and marketing blood test products +700 peer reviewed publications. under the suPARnostic® brand for better triaging in hospitals to: ViroGates has patented the clinical use of suPAR levels as a risk stratification method and currently holds 4 granted patent families and one newly filed application (2018). improve patient care reduce healthcare costs AUTO Flex ELISA Quick Triage TurbiLatex empower clinical staff Application R&D & clinical Near-patient Automated, use lab centralized lab ViroGates was founded in 2001 and has since 2018 been listed on Nasdaq First North Growth Market DK – Ticker Technology ELISA Lateral flow Turbidimetric “VIRO” Launch year 2009 2015 2018 (CE-IVD) ViroGates 3 suPAR is a marker of immune activation and a strong predictor disease severity and progession uPAR suPAR suPARnostic® +700 peer (soluble urokinase Plasminogen Activator Receptor) suPAR DI DI DII DII reviewed papers supporting A prognostic inflammatory DIII DIII evidence biomarker for immune activation indicating: Kidney disease Liver disease • Disease presence (acute, Diabetes Elevated -
(ΐ2) United States Patent (ΐο) Patent No.: US 10,596,255 Β2 Clube (45) Date of Patent: *Mar
US010596255B2 US010596255B2 (ΐ2) United States Patent (ΐο) Patent No.: US 10,596,255 Β2 Clube (45) Date of Patent: *Mar. 24, 2020 (54) SELECTIVELY ALTERING MICROBIOTA 39/0258; C07K 14/315; C07K 14/245; FOR IMMUNE MODULATION C07K 14/285; C07K 14/295; C12N 2310/20; C12N 2320/30 (71) Applicant: SNIPR Technologies Limited, London See application file for complete search history. (GB) (72) Inventor: Jasper Clube, London (GB) (56) References Cited (73) Assignee: SNIPR Technologies Limited, London U.S. PATENT DOCUMENTS (GB) 4,626,504 A 12/1986 Puhler et al. 5,633,154 A 5/1997 Schaefer et al. (*) Notice: Subject to any disclaimer, the term of this 8,241,498 Β2 8/2012 Summer et al. patent is extended or adjusted under 35 8,252,576 Β2 8/2012 Campbell et al. U.S.C. 154(b) by 0 days. 8,906,682 Β2 12/2014 June et al. 8,911,993 Β2 12/2014 June et al. This patent is subject to a terminal dis 8,916,381 Β1 12/2014 June et al. 8,975,071 Β1 3/2015 June et al. claimer. 9,101,584 Β2 8/2015 June et al. 9,102,760 Β2 8/2015 June et al. (21) Appl. No.: 16/389,376 9,102,761 Β2 8/2015 June et al. 9,113,616 Β2 8/2015 MacDonald et al. (22) Filed: Apr. 19, 2019 9,328,156 Β2 5/2016 June et al. 9,464,140 Β2 10/2016 June et al. (65) Prior Publication Data 9,481,728 Β2 11/2016 June et al. -
Of Body Inflammation for Maxillofacial Surgery Purpose-To Make
applied sciences Article Index of Body Inflammation for Maxillofacial Surgery Purpose-to Make the Soluble Urokinase-Type Plasminogen Activator Receptor Serum Level Independent on Patient Age Marcin Kozakiewicz 1,*, Magdalena Trzci´nska-Kubik 1 and Rafał Nikodem Wlazeł 2 1 Department of Maxillofacial Surgery, Medical University of Lodz, 113 Zeromskiego˙ Str., 90-549 Lodz, Poland; [email protected] 2 Department of Laboratory Diagnostics and Clinical Biochemistry, Medical University of Lodz, 251 Pomorska Str., 92-213 Lodz, Poland; [email protected] * Correspondence: [email protected]; Tel.: +48-42-6393068 Featured Application: Inflammation is still a threat to patients of all age groups. Monitoring the infection is essential to control it. The soluble urokinase-type plasminogen activator receptor (suPAR) is a very sensitive marker. Maxillofacial surgery is one of the fields of medicine widely fighting against infections. The concentration of suPAR holds an independent information for risk stratification of morbidity and mortality in various acute and chronic diseases. The use of an ultra-sensitive measure of the development of inflammation and at the same time independent on the patient’s age would be clinically very beneficial. Abstract: Background: The serum suPAR level is affected in humans by it increases with age. Therefore it makes difficult interpretation and any comparison of age varied groups. The aim of this Citation: Kozakiewicz, M.; study is to find simple way to age independent presentation of suPAR serum level for maxillofacial Trzci´nska-Kubik,M.; Wlazeł, R.N. surgery purpose. Methods: In generally healthy patients from 15 to 59 y.o. suPAR level was tested Index of Body Inflammation for in serum before orthognathic or minor traumatologic procedures. -
Diversity and Distribution of Actinobacteria Associated with Reef Coral Porites Lutea
View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Frontiers - Publisher Connector ORIGINAL RESEARCH published: 21 October 2015 doi: 10.3389/fmicb.2015.01094 Diversity and distribution of Actinobacteria associated with reef coral Porites lutea Weiqi Kuang 1, 2 †, Jie Li 1 †, Si Zhang 1 and Lijuan Long 1* 1 CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, China, 2 College of Earth Science, University of Chinese Academy of Sciences, Beijing, China Actinobacteria is a ubiquitous major group in coral holobiont. The diversity and spatial Edited by: and temporal distribution of actinobacteria have been rarely documented. In this Sheng Qin, study, diversity of actinobacteria associated with mucus, tissue and skeleton of Porites Jiangsu Normal University, China lutea and in the surrounding seawater were examined every 3 months for 1 year on Reviewed by: Syed Gulam Dastager, Luhuitou fringing reef. The population structures of the P.lutea-associated actinobacteria National Collection of Industrial were analyzed using phylogenetic analysis of 16S rRNA gene clone libraries, which Microorganisms Resource Center, demonstrated highly diverse actinobacteria profiles in P. lutea. A total of 25 described India Wei Sun, families and 10 unnamed families were determined in the populations, and 12 genera Shanghai Jiao Tong University, China were firstly detected in corals. The Actinobacteria diversity was significantly different P. Nithyanand, SASTRA University, India between the P. lutea and the surrounding seawater. Only 10 OTUs were shared by *Correspondence: the seawater and coral samples. -
Polycyclic Aromatic Hydrocarbon Degrading Bacteria from the Indonesian Marine Environment
BIODIVERSITAS ISSN: 1412-033X Volume 17, Number 2, October 2016 E-ISSN: 2085-4722 Pages: 857-864 DOI: 10.13057/biodiv/d170263 Polycyclic aromatic hydrocarbon degrading bacteria from the Indonesian Marine Environment ELVI YETTI♥, AHMAD THONTOWI, YOPI Laboratory of Biocatalyst and Fermentation, Research Centre for Biotechnology, Indonesian Institute of Sciences. Cibinong Science Center, Jl. Raya Bogor Km 46 Cibinong-Bogor 16911, West Java, Indonesia. Tel. +62-21-8754587, Fax. +62-21-8754588, ♥email: [email protected] Manuscript received: 20 April 2016. Revision accepted: 20 October 2016. Abstract. Yetti E, Thontowi A, Yopi. 2016. Polyaromatic hydrocarbon degrading bacteria from the Indonesian Marine Environment. Biodiversitas 17: 857-864. Oil spills are one of the main causes of pollution in marine environments. Oil degrading bacteria play an important role for bioremediation of oil spill in environment. We collected 132 isolates of marine bacteria isolated from several Indonesia marine areas, i.e. Pari Island, Jakarta, Kamal Port, East Java and Cilacap Bay, Central Java. These isolates were screened for capability to degrade polyaromatic hydrocarbons (PAHs). Selection test were carried out qualitatively using sublimation method and growth assay of the isolates on several PAHs i.e. phenanthrene, dibenzothiophene, fluorene, naphtalene, phenotiazine, and pyrene. The fifty-eight isolates indicated in having capability to degrade PAHs, consisted of 25 isolates were positive on naphthalene (nap) and 20 isolates showed ability to grow in phenanthrene (phen) containing media. Further, 38 isolates were selected for dibenzothiophene (dbt) degradation and 25 isolates were positive on fluorene (flr). On the other hand, 23 isolates presented capability to degrade in phenothiazine (ptz) and 15 isolates could grow in media with pyrene (pyr).