Multi Locus Sequence Analysis of Root Nodule Isolates from Lotus
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UNIVERSIDAD POLITÉCNICA DE CARTAGENA ESCUELA TÉCNICA SUPERIOR DE INGENIERÍA AGRONÓMICA DEPARTAMENTO DE PRODUCCIÓN VEGETAL INGENIERO AGRÓNOMO PROYECTO FIN DE CARRERA: “AISLAMIENTO E IDENTIFICACIÓN DE LOS RIZOBIOS ASOCIADOS A LOS NÓDULOS DE ASTRAGALUS NITIDIFLORUS”. Realizado por: Noelia Real Giménez Dirigido por: María José Vicente Colomer Francisco José Segura Carreras Cartagena, Julio de 2014. ÍNDICE GENERAL 1. Introducción…………………………………………………….…………………………………………………1 1.1. Astragalus nitidiflorus………………………………..…………………………………………………2 1.1.1. Encuadre taxonómico……………………………….…..………………………………………………2 1.1.2. El origen de Astragalus nitidiflorus………………………………………………………………..4 1.1.3. Descripción de la especie………..…………………………………………………………………….5 1.1.4. Biología…………………………………………………………………………………………………………7 1.1.4.1. Ciclo vegetativo………………….……………………………………………………………………7 1.1.4.2. Fenología de la floración……………………………………………………………………….9 1.1.4.3. Sistema de reproducción……………………………………………………………………….10 1.1.4.4. Dispersión de los frutos…………………………………….…………………………………..11 1.1.4.5. Nodulación con Rhizobium…………………………………………………………………….12 1.1.4.6. Diversidad genética……………………………………………………………………………....13 1.1.5. Ecología………………………………………………………………………………………………..…….14 1.1.6. Corología y tamaño poblacional……………………………………………………..…………..15 1.1.7. Protección…………………………………………………………………………………………………..18 1.1.8. Amenazas……………………………………………………………………………………………………19 1.1.8.1. Factores bióticos…………………………………………………………………………………..19 1.1.8.2. Factores abióticos………………………………………………………………………………….20 1.1.8.3. Factores antrópicos………………..…………………………………………………………….21 -
Actes Congrès MICROBIOD 1
Actes du congrès international "Biotechnologie microbienne au service du développement" (MICROBIOD) Marrakech, MAROC 02-05 Novembre 2009 "Ce travail est publié avec le soutien du Ministère de l'Education Nationale, de l'Enseignement Supérieur, de la Formation des Cadres et de la Recherche Scientifique et du CNRST". MICROBIONA Edition www.ucam.ac.ma/microbiona 1 1 Mot du Comité d'organisation Cher (e) membre de MICROBIONA Cher(e) Participant(e), Sous le Haut Patronage de sa Majesté le Roi Mohammed VI, l'Association MICROBIONA et la Faculté des Sciences Semlalia, Université Cadi Ayyad, organisent du 02 au 05 Novembre 2009, en collaboration avec la Société Française de Microbiologie, le Pôle de Compétences Eau et Environnement et l'Incubateur Universitaire de Marrakech, le congrès international "Biotechnologie microbienne au service du développement" (MICROBIOD). Cette manifestation scientifique spécialisée permettra la rencontre de chercheurs de renommée internationale dans le domaine de la biotechnologie microbienne. Le congrès MICROBIOD est une opportunité pour les participants de mettre en relief l’importance socio-économique et environnementale de la valorisation et l’application de nouvelles techniques de biotechnologie microbienne dans divers domaines appliquées au développement et la gestion durable des ressources, à savoir l’agriculture, l'alimentation, l'environnement, la santé, l’industrie agro-alimentaire et le traitement et recyclage des eaux et des déchets par voie microbienne. Ce congrès sera également l’occasion pour les enseignants-chercheurs et les étudiants-chercheurs marocains d’actualiser leurs connaissances dans le domaine des biotechnologies microbiennes, qui serviront à l’amélioration de leurs recherches et enseignements. Le congrès MICROBIOD sera également l’occasion pour certains de nos collaborateurs étrangers de contribuer de près à la formation de nos étudiants en biotechnologie microbienne et ceci par la discussion des protocoles de travail, des méthodes d’analyse et des résultats obtenus. -
Revised Taxonomy of the Family Rhizobiaceae, and Phylogeny of Mesorhizobia Nodulating Glycyrrhiza Spp
Division of Microbiology and Biotechnology Department of Food and Environmental Sciences University of Helsinki Finland Revised taxonomy of the family Rhizobiaceae, and phylogeny of mesorhizobia nodulating Glycyrrhiza spp. Seyed Abdollah Mousavi Academic Dissertation To be presented, with the permission of the Faculty of Agriculture and Forestry of the University of Helsinki, for public examination in lecture hall 3, Viikki building B, Latokartanonkaari 7, on the 20th of May 2016, at 12 o’clock noon. Helsinki 2016 Supervisor: Professor Kristina Lindström Department of Environmental Sciences University of Helsinki, Finland Pre-examiners: Professor Jaakko Hyvönen Department of Biosciences University of Helsinki, Finland Associate Professor Chang Fu Tian State Key Laboratory of Agrobiotechnology College of Biological Sciences China Agricultural University, China Opponent: Professor J. Peter W. Young Department of Biology University of York, England Cover photo by Kristina Lindström Dissertationes Schola Doctoralis Scientiae Circumiectalis, Alimentariae, Biologicae ISSN 2342-5423 (print) ISSN 2342-5431 (online) ISBN 978-951-51-2111-0 (paperback) ISBN 978-951-51-2112-7 (PDF) Electronic version available at http://ethesis.helsinki.fi/ Unigrafia Helsinki 2016 2 ABSTRACT Studies of the taxonomy of bacteria were initiated in the last quarter of the 19th century when bacteria were classified in six genera placed in four tribes based on their morphological appearance. Since then the taxonomy of bacteria has been revolutionized several times. At present, 30 phyla belong to the domain “Bacteria”, which includes over 9600 species. Unlike many eukaryotes, bacteria lack complex morphological characters and practically phylogenetically informative fossils. It is partly due to these reasons that bacterial taxonomy is complicated. -
Defining the Rhizobium Leguminosarum Species Complex
Preprints (www.preprints.org) | NOT PEER-REVIEWED | Posted: 12 December 2020 doi:10.20944/preprints202012.0297.v1 Article Defining the Rhizobium leguminosarum species complex J. Peter W. Young 1,*, Sara Moeskjær 2, Alexey Afonin 3, Praveen Rahi 4, Marta Maluk 5, Euan K. James 5, Maria Izabel A. Cavassim 6, M. Harun-or Rashid 7, Aregu Amsalu Aserse 8, Benjamin J. Perry 9, En Tao Wang 10, Encarna Velázquez 11, Evgeny E. Andronov 12, Anastasia Tampakaki 13, José David Flores Félix 14, Raúl Rivas González 11, Sameh H. Youseif 15, Marc Lepetit 16, Stéphane Boivin 16, Beatriz Jorrin 17, Gregory J. Kenicer 18, Álvaro Peix 19, Michael F. Hynes 20, Martha Helena Ramírez-Bahena 21, Arvind Gulati 22 and Chang-Fu Tian 23 1 Department of Biology, University of York, York YO10 5DD, UK 2 Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark; [email protected] 3 Laboratory for genetics of plant-microbe interactions, ARRIAM, Pushkin, 196608 Saint-Petersburg, Russia; [email protected] 4 National Centre for Microbial Resource, National Centre for Cell Science, Pune, India; [email protected] 5 Ecological Sciences, The James Hutton Institute, Invergowrie, Dundee DD2 5DA, UK; [email protected] (M.M.); [email protected] (E.K.J.) 6 Department of Ecology and Evolutionary Biology, University of California, Los Angeles, CA 90095, USA; [email protected] 7 Biotechnology Division, Bangladesh Institute of Nuclear Agriculture (BINA), Bangladesh; [email protected] 8 Ecosystems and Environment Research programme , Faculty of Biological and Environmental Sciences, University of Helsinki, FI-00014 Finland; [email protected] 9 Department of Microbiology and Immunology, University of Otago, Dunedin 9016, New Zealand; [email protected] 10 Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Cd. -
Specificity in Legume-Rhizobia Symbioses
International Journal of Molecular Sciences Review Specificity in Legume-Rhizobia Symbioses Mitchell Andrews * and Morag E. Andrews Faculty of Agriculture and Life Sciences, Lincoln University, PO Box 84, Lincoln 7647, New Zealand; [email protected] * Correspondence: [email protected]; Tel.: +64-3-423-0692 Academic Editors: Peter M. Gresshoff and Brett Ferguson Received: 12 February 2017; Accepted: 21 March 2017; Published: 26 March 2017 Abstract: Most species in the Leguminosae (legume family) can fix atmospheric nitrogen (N2) via symbiotic bacteria (rhizobia) in root nodules. Here, the literature on legume-rhizobia symbioses in field soils was reviewed and genotypically characterised rhizobia related to the taxonomy of the legumes from which they were isolated. The Leguminosae was divided into three sub-families, the Caesalpinioideae, Mimosoideae and Papilionoideae. Bradyrhizobium spp. were the exclusive rhizobial symbionts of species in the Caesalpinioideae, but data are limited. Generally, a range of rhizobia genera nodulated legume species across the two Mimosoideae tribes Ingeae and Mimoseae, but Mimosa spp. show specificity towards Burkholderia in central and southern Brazil, Rhizobium/Ensifer in central Mexico and Cupriavidus in southern Uruguay. These specific symbioses are likely to be at least in part related to the relative occurrence of the potential symbionts in soils of the different regions. Generally, Papilionoideae species were promiscuous in relation to rhizobial symbionts, but specificity for rhizobial genus appears to hold at the tribe level for the Fabeae (Rhizobium), the genus level for Cytisus (Bradyrhizobium), Lupinus (Bradyrhizobium) and the New Zealand native Sophora spp. (Mesorhizobium) and species level for Cicer arietinum (Mesorhizobium), Listia bainesii (Methylobacterium) and Listia angolensis (Microvirga). -
Common Commensals
Common Commensals Actinobacterium meyeri Aerococcus urinaeequi Arthrobacter nicotinovorans Actinomyces Aerococcus urinaehominis Arthrobacter nitroguajacolicus Actinomyces bernardiae Aerococcus viridans Arthrobacter oryzae Actinomyces bovis Alpha‐hemolytic Streptococcus, not S pneumoniae Arthrobacter oxydans Actinomyces cardiffensis Arachnia propionica Arthrobacter pascens Actinomyces dentalis Arcanobacterium Arthrobacter polychromogenes Actinomyces dentocariosus Arcanobacterium bernardiae Arthrobacter protophormiae Actinomyces DO8 Arcanobacterium haemolyticum Arthrobacter psychrolactophilus Actinomyces europaeus Arcanobacterium pluranimalium Arthrobacter psychrophenolicus Actinomyces funkei Arcanobacterium pyogenes Arthrobacter ramosus Actinomyces georgiae Arthrobacter Arthrobacter rhombi Actinomyces gerencseriae Arthrobacter agilis Arthrobacter roseus Actinomyces gerenseriae Arthrobacter albus Arthrobacter russicus Actinomyces graevenitzii Arthrobacter arilaitensis Arthrobacter scleromae Actinomyces hongkongensis Arthrobacter astrocyaneus Arthrobacter sulfonivorans Actinomyces israelii Arthrobacter atrocyaneus Arthrobacter sulfureus Actinomyces israelii serotype II Arthrobacter aurescens Arthrobacter uratoxydans Actinomyces meyeri Arthrobacter bergerei Arthrobacter ureafaciens Actinomyces naeslundii Arthrobacter chlorophenolicus Arthrobacter variabilis Actinomyces nasicola Arthrobacter citreus Arthrobacter viscosus Actinomyces neuii Arthrobacter creatinolyticus Arthrobacter woluwensis Actinomyces odontolyticus Arthrobacter crystallopoietes -
Light Modulates Important Physiological Features of Ralstonia
www.nature.com/scientificreports OPEN Light modulates important physiological features of Ralstonia pseudosolanacearum during the colonization of tomato plants Josefna Tano1,6, María Belén Ripa1,6, María Laura Tondo2, Analía Carrau1, Silvana Petrocelli2, María Victoria Rodriguez3, Virginia Ferreira4, María Inés Siri4, Laura Piskulic5 & Elena Graciela Orellano1* Ralstonia pseudosolanacearum GMI1000 (Rpso GMI1000) is a soil-borne vascular phytopathogen that infects host plants through the root system causing wilting disease in a wide range of agro- economic interest crops, producing economical losses. Several features contribute to the full bacterial virulence. In this work we study the participation of light, an important environmental factor, in the regulation of the physiological attributes and infectivity of Rpso GMI1000. In silico analysis of the Rpso genome revealed the presence of a Rsp0254 gene, which encodes a putative blue light LOV-type photoreceptor. We constructed a mutant strain of Rpso lacking the LOV protein and found that the loss of this protein and light, infuenced characteristics involved in the pathogenicity process such as motility, adhesion and the bioflms development, which allows the successful host plant colonization, rendering bacterial wilt. This protein could be involved in the adaptive responses to environmental changes. We demonstrated that light sensing and the LOV protein, would be used as a location signal in the host plant, to regulate the expression of several virulence factors, in a time and tissue dependent way. Consequently, bacteria could use an external signal and Rpsolov gene to know their location within plant tissue during the colonization process. Light is an important environmental factor in all ecosystems because it is a source of energy and information. -
Analysis of the Interaction Between Pisum Sativum L. and Rhizobium Laguerreae Strains Nodulating This Legume in Northwest Spain
plants Article Analysis of the Interaction between Pisum sativum L. and Rhizobium laguerreae Strains Nodulating This Legume in Northwest Spain 1, 1 1, 2 José David Flores-Félix y , Lorena Carro , Eugenia Cerda-Castillo z, Andrea Squartini , Raúl Rivas 1,3,4 and Encarna Velázquez 1,3,4,* 1 Departamento de Microbiologíay Genética, Universidad de Salamanca, 37007 Salamanca, Spain; jdfl[email protected] (J.D.F.-F.); [email protected] (L.C.); [email protected] (E.C.-C.); [email protected] (R.R.) 2 Department of Agronomy, Food, Natural Resources, Animals and Environment, University of Padova, 35020 Legnaro, Italy; [email protected] 3 Instituto Hispanoluso de Investigaciones Agrarias, 37007 Salamanca, Spain 4 Unidad Asociada USAL-IRNASA, 37007 Salamanca, Spain * Correspondence: [email protected]; Tel.: +349-2329-4532 Present address: CICS-UBI–Health Sciences Research Centre, University of Beira Interior, y 6200-506 Covilhã, Portugal. Present address: Departamento de Biología, Facultad de Ciencia y Tecnología, Universidad Nacional z Autónoma de Nicaragua, León 21000, Nicaragua. Received: 3 November 2020; Accepted: 9 December 2020; Published: 11 December 2020 Abstract: Pisum sativum L. (pea) is one of the most cultivated grain legumes in European countries due to the high protein content of its seeds. Nevertheless, the rhizobial microsymbionts of this legume have been scarcely studied in these countries. In this work, we analyzed the rhizobial strains nodulating the pea in a region from Northwestern Spain, where this legume is widely cultivated. The isolated strains were genetically diverse, and the phylogenetic analysis of core and symbiotic genes showed that these strains belong to different clusters related to R. -
Characterisation of Bacteria Associated with the Root Nodules of Hypocalyptus and Related Genera
Characterisation of bacteria associated with the root nodules of Hypocalyptus and related genera by Chrizelle Winsie Beukes Dissertation submitted in partial fulfilment of the requirements for the degree Magister Scientiae In the Faculty of Natural and Agricultural Sciences, Department of Microbiology and Plant Pathology, Forestry and Agricultural Biotechnology Institute, University of Pretoria, Pretoria, South Africa Promoter: Prof. E.T. Steenkamp Co-promoters: Prof. S.N. Venter Dr. I.J. Law August 2011 © University of Pretoria Dedicated to my parents, Hendrik and Lorraine. Thank you for your unwavering support. © University of Pretoria I certify that this dissertation hereby submitted to the University of Pretoria for the degree of Magister Scientiae (Microbiology), has not previously been submitted by me in respect of a degree at any other university. Signature _________________ August 2011 © University of Pretoria Table of Contents Acknowledgements i Preface ii Chapter 1 1 Taxonomy, infection biology and evolution of rhizobia, with special reference to those nodulating Hypocalyptus Chapter 2 80 Diverse beta-rhizobia nodulate legumes in the South African indigenous tribe Hypocalypteae Chapter 3 131 African origins for fynbos associated beta-rhizobia Summary 173 © University of Pretoria Acknowledgements Firstly I want to acknowledge Our Heavenly Father, for granting me the opportunity to obtain this degree and for putting the special people along my way to aid me in achieving it. Then I would like to take the opportunity to thank the following people and institutions: My parents, Hendrik and Lorraine, thank you for your support, understanding and love; Prof. Emma Steenkamp, for her guidance, advice and significant insights throughout this project; My co-supervisors, Prof. -
Mesorhizobium Septentrionale Sp Nov and Mesorhizobium Temperatum Sp Nov., Isolated from Astragalus Adsurgens Growing in the Northern Regions of China
This is a repository copy of Mesorhizobium septentrionale sp nov and Mesorhizobium temperatum sp nov., isolated from Astragalus adsurgens growing in the northern regions of China. White Rose Research Online URL for this paper: https://eprints.whiterose.ac.uk/290/ Article: Gao, J-L., Turner, S.L., Kan, F.L. et al. (8 more authors) (2004) Mesorhizobium septentrionale sp nov and Mesorhizobium temperatum sp nov., isolated from Astragalus adsurgens growing in the northern regions of China. International Journal of Systematic and Evolutionary Microbiology. pp. 2003-2012. ISSN 1466-5034 https://doi.org/10.1099/ijs.0.02840-0 Reuse Items deposited in White Rose Research Online are protected by copyright, with all rights reserved unless indicated otherwise. They may be downloaded and/or printed for private study, or other acts as permitted by national copyright laws. The publisher or other rights holders may allow further reproduction and re-use of the full text version. This is indicated by the licence information on the White Rose Research Online record for the item. Takedown If you consider content in White Rose Research Online to be in breach of UK law, please notify us by emailing [email protected] including the URL of the record and the reason for the withdrawal request. [email protected] https://eprints.whiterose.ac.uk/ International Journal of Systematic and Evolutionary Microbiology (2004), 54, 2003–2012 DOI 10.1099/ijs.0.02840-0 Mesorhizobium septentrionale sp. nov. and Mesorhizobium temperatum sp. nov., isolated from Astragalus adsurgens growing in the northern regions of China Jun-Lian Gao,1,2,3 Sarah Lea Turner,3 Feng Ling Kan,1 En Tao Wang,1,4 Zhi Yuan Tan,5 Yu Hui Qiu,1 Jun Gu,1 Zewdu Terefework,2 J. -
Identifying Elite Rhizobia for Commercial Soybean
IDENTIFYING ELITE RHIZOBIA FOR COMMERCIAL SOYBEAN (GLYCINE MAX) INOCULANTS MAUREEN N. WASWA BSc Horticulture (JKUAT) THESIS SUBMITTED IN PARTIAL FULFILMENT FOR THE AWARD OF DEGREE OF MASTER OF SCIENCE IN SOIL SCIENCE DEPARTMENT OF LAND RESOURCE MANAGEMENT AND AGRICULTURAL TECHNOLOGY, UNIVERSITY OF NAIROBI 2013 1 DECLARATION This thesis is my original work and has not been submitted for a degree in any other University Signature………………….. Date…………………… Maureen Nekoye Waswa This thesis has been submitted for examination with our approval as supervisors 1. Signature……………… Date…………………… Prof. Nancy Karanja (UoN) 2. Signature……………… Date…………………… Dr. Paul Woomer (CIAT-TSBF) 3. Signature……………… Date…………………… Dr. Frederick Baijukya (CIAT-TSBF) i DEDICATION To: my father, Chrispus W. Wanjala; my mother, Norah N. Wanjala; and my brothers and sisters. My parents’ vision for a better tomorrow is unrivalled by the best educators in my life. Above all I extend my sincere gratitude to the Almighty God for making this journey possible. ii ACKNOWLEDGEMENT I take this opportunity to express my gratitude to my supervisors Prof. K.N Karanja (University of Nairobi), Dr. Paul L. Woomer (CIAT-TSBF) and Dr. Frederick Baijukya (CIAT-TSBF) for their constant expert guidance, advice and support through all the stages of the research work. I also wish to thank all technicians in the department of LARMAT particularly Stanley Kisamuli for the encouragement and support provided during my research. My gratitude also goes to the Bill and Melinda Gates Foundation for funding my course and research work through N2Africa project. Appreciation also goes to my husband, Andrew S. Mabonga for his support during long period of my study. -
Identification of Small Non-Coding Rnas from Rhizobium Etli By
Rajendran et al. ExRNA (2020) 2:14 https://doi.org/10.1186/s41544-020-00054-1 ExRNA RESEARCH Open Access Identification of small non-coding RNAs from Rhizobium etli by integrated genome- wide and transcriptome-based methods Kasthuri Rajendran1, Vikram Kumar2, Ilamathi Raja1, Manoharan Kumariah1 and Jebasingh Tennyson2* Abstract Background: Small non-coding RNAs (sRNAs) are regulatory molecules, present in all forms of life, known to regulate various biological processes in response to the different environmental signals. In recent years, deep sequencing and various other computational prediction methods have been employed to identify and analyze sRNAs. Results: In the present study, we have applied an improved sRNA scanner method to predict sRNAs from the genome of Rhizobium etli, based on PWM matrix of conditional sigma factor 32. sRNAs predicted from the genome are integrated with the available stress specific transcriptome data to predict putative conditional specific sRNAs. A total of 271 sRNAs from the genome and 173 sRNAs from the transcriptome are computationally predicted. Of these, 25 sRNAs are found in both genome and transcriptome data. Putative targets for these sRNAs are predicted using TargetRNA2 and these targets are involved in a wide array of cellular functions such as cell division, transport and metabolism of amino acids, carbohydrates, energy production and conversion, translation, cell wall/membrane biogenesis, post- translation modification, protein turnover and chaperones. Predicted targets are functionally classified based on COG analysis and GO annotations. Conclusion: sRNAs predicted from the genome, using PWM matrices for conditional sigma factor 32 could be a better method to identify the conditional specific sRNAs which expand the list of putative sRNAs from the intergenic regions (IgRs) of R.