'Sick Building Syndrome' Can Be Produced by Wet Wall Fungi
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FINAL RISK ASSESSMENT for Aspergillus Niger (February 1997)
ATTACHMENT I--FINAL RISK ASSESSMENT FOR Aspergillus niger (February 1997) I. INTRODUCTION Aspergillus niger is a member of the genus Aspergillus which includes a set of fungi that are generally considered asexual, although perfect forms (forms that reproduce sexually) have been found. Aspergilli are ubiquitous in nature. They are geographically widely distributed, and have been observed in a broad range of habitats because they can colonize a wide variety of substrates. A. niger is commonly found as a saprophyte growing on dead leaves, stored grain, compost piles, and other decaying vegetation. The spores are widespread, and are often associated with organic materials and soil. History of Commercial Use and Products Subject to TSCA Jurisdiction The primary uses of A. niger are for the production of enzymes and organic acids by fermentation. While the foods, for which some of the enzymes may be used in preparation, are not subject to TSCA, these enzymes may have multiple uses, many of which are not regulated except under TSCA. Fermentations to produce these enzymes may be carried out in vessels as large as 100,000 liters (Finkelstein et al., 1989). A. niger is also used to produce organic acids such as citric acid and gluconic acid. The history of safe use for A. niger comes primarily from its use in the food industry for the production of many enzymes such as a-amylase, amyloglucosidase, cellulases, lactase, invertase, pectinases, and acid proteases (Bennett, 1985a; Ward, 1989). In addition, the annual production of citric acid by fermentation is now approximately 350,000 tons, using either A. -
Allergic Bronchopulmonary Aspergillosis and Severe Asthma with Fungal Sensitisation
Allergic Bronchopulmonary Aspergillosis and Severe Asthma with Fungal Sensitisation Dr Rohit Bazaz National Aspergillosis Centre, UK Manchester University NHS Foundation Trust/University of Manchester ~ ABPA -a41'1 Severe asthma wl'th funga I Siens itisat i on Subacute IA Chronic pulmonary aspergillosjs Simp 1Ie a:spe rgmoma As r§i · bronchitis I ram une dysfu net Ion Lun· damage Immu11e hypce ractivitv Figure 1 In t@rarctfo n of Aspergillus Vliith host. ABP A, aHerg tc broncho pu~ mo na my as µe rgi ~fos lis; IA, i nvas we as ?@rgiH os 5. MANCHl·.'>I ER J:-\2 I Kosmidis, Denning . Thorax 2015;70:270–277. doi:10.1136/thoraxjnl-2014-206291 Allergic Fungal Airway Disease Phenotypes I[ Asthma AAFS SAFS ABPA-S AAFS-asthma associated with fu ngaIsensitization SAFS-severe asthma with funga l sensitization ABPA-S-seropositive a llergic bronchopulmonary aspergi ll osis AB PA-CB-all ergic bronchopulmonary aspergi ll osis with central bronchiectasis Agarwal R, CurrAlfergy Asthma Rep 2011;11:403 Woolnough K et a l, Curr Opin Pulm Med 2015;21:39 9 Stanford Lucile Packard ~ Children's. Health Children's. Hospital CJ Scanford l MEDICINE Stanford MANCHl·.'>I ER J:-\2 I Aspergi 11 us Sensitisation • Skin testing/specific lgE • Surface hydroph,obins - RodA • 30% of patients with asthma • 13% p.atients with COPD • 65% patients with CF MANCHl·.'>I ER J:-\2 I Alternar1a• ABPA •· .ABPA is an exagg·erated response ofthe imm1une system1 to AspergUlus • Com1pUcatio n of asthm1a and cystic f ibrosis (rarell·y TH2 driven COPD o r no identif ied p1 rior resp1 iratory d isease) • ABPA as a comp1 Ucation of asth ma affects around 2.5% of adullts. -
Monoclonal Antibodies As Tools to Combat Fungal Infections
Journal of Fungi Review Monoclonal Antibodies as Tools to Combat Fungal Infections Sebastian Ulrich and Frank Ebel * Institute for Infectious Diseases and Zoonoses, Faculty of Veterinary Medicine, Ludwig-Maximilians-University, D-80539 Munich, Germany; [email protected] * Correspondence: [email protected] Received: 26 November 2019; Accepted: 31 January 2020; Published: 4 February 2020 Abstract: Antibodies represent an important element in the adaptive immune response and a major tool to eliminate microbial pathogens. For many bacterial and viral infections, efficient vaccines exist, but not for fungal pathogens. For a long time, antibodies have been assumed to be of minor importance for a successful clearance of fungal infections; however this perception has been challenged by a large number of studies over the last three decades. In this review, we focus on the potential therapeutic and prophylactic use of monoclonal antibodies. Since systemic mycoses normally occur in severely immunocompromised patients, a passive immunization using monoclonal antibodies is a promising approach to directly attack the fungal pathogen and/or to activate and strengthen the residual antifungal immune response in these patients. Keywords: monoclonal antibodies; invasive fungal infections; therapy; prophylaxis; opsonization 1. Introduction Fungal pathogens represent a major threat for immunocompromised individuals [1]. Mortality rates associated with deep mycoses are generally high, reflecting shortcomings in diagnostics as well as limited and often insufficient treatment options. Apart from the development of novel antifungal agents, it is a promising approach to activate antimicrobial mechanisms employed by the immune system to eliminate microbial intruders. Antibodies represent a major tool to mark and combat microbes. Moreover, monoclonal antibodies (mAbs) are highly specific reagents that opened new avenues for the treatment of cancer and other diseases. -
MM 0839 REV0 0918 Idweek 2018 Mucor Abstract Poster FINAL
Invasive Mucormycosis Management: Mucorales PCR Provides Important, Novel Diagnostic Information Kyle Wilgers,1 Joel Waddell,2 Aaron Tyler,1 J. Allyson Hays,2,3 Mark C. Wissel,1 Michelle L. Altrich,1 Steve Kleiboeker,1 Dwight E. Yin2,3 1 Viracor Eurofins Clinical Diagnostics, Lee’s Summit, MO 2 Children’s Mercy, Kansas City, MO 3 University of Missouri-Kansas City School of Medicine, Kansas City, MO INTRODUCTION RESULTS Early diagnosis and treatment of invasive mucormycosis (IM) affects patient MUC PCR results of BAL submitted for Aspergillus testing. The proportions of Case study of IM confirmed by MUC PCR. A 12 year-old boy with multiply relapsed pre- outcomes. In immunocompromised patients, timely diagnosis and initiation of appropriate samples positive for Mucorales and Aspergillus in BAL specimens submitted for IA testing B cell acute lymphoblastic leukemia, despite extensive chemotherapy, two allogeneic antifungal therapy are critical to improving survival and reducing morbidity (Chamilos et al., are compared in Table 2. Out of 869 cases, 12 (1.4%) had POS MUC PCR, of which only hematopoietic stem cell transplants, and CAR T-cell therapy, presented with febrile 2008; Kontoyiannis et al., 2014; Walsh et al., 2012). two had been ordered for MUC PCR. Aspergillus was positive in 56/869 (6.4%) of neutropenia (0 cells/mm3), cough, and right shoulder pain while on fluconazole patients, with 5/869 (0.6%) positive for Aspergillus fumigatus and 50/869 (5.8%) positive prophylaxis. Chest CT revealed a right lung cavity, which ultimately became 5.6 x 6.2 x 5.9 Differentiating diagnosis between IM and invasive aspergillosis (IA) affects patient for Aspergillus terreus. -
Allergic Bronchopulmonary Aspergillosis: a Perplexing Clinical Entity Ashok Shah,1* Chandramani Panjabi2
Review Allergy Asthma Immunol Res. 2016 July;8(4):282-297. http://dx.doi.org/10.4168/aair.2016.8.4.282 pISSN 2092-7355 • eISSN 2092-7363 Allergic Bronchopulmonary Aspergillosis: A Perplexing Clinical Entity Ashok Shah,1* Chandramani Panjabi2 1Department of Pulmonary Medicine, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi, India 2Department of Respiratory Medicine, Mata Chanan Devi Hospital, New Delhi, India This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. In susceptible individuals, inhalation of Aspergillus spores can affect the respiratory tract in many ways. These spores get trapped in the viscid spu- tum of asthmatic subjects which triggers a cascade of inflammatory reactions that can result in Aspergillus-induced asthma, allergic bronchopulmo- nary aspergillosis (ABPA), and allergic Aspergillus sinusitis (AAS). An immunologically mediated disease, ABPA, occurs predominantly in patients with asthma and cystic fibrosis (CF). A set of criteria, which is still evolving, is required for diagnosis. Imaging plays a compelling role in the diagno- sis and monitoring of the disease. Demonstration of central bronchiectasis with normal tapering bronchi is still considered pathognomonic in pa- tients without CF. Elevated serum IgE levels and Aspergillus-specific IgE and/or IgG are also vital for the diagnosis. Mucoid impaction occurring in the paranasal sinuses results in AAS, which also requires a set of diagnostic criteria. Demonstration of fungal elements in sinus material is the hall- mark of AAS. -
Characterization of Terrelysin, a Potential Biomarker for Aspergillus Terreus
Graduate Theses, Dissertations, and Problem Reports 2012 Characterization of terrelysin, a potential biomarker for Aspergillus terreus Ajay Padmaj Nayak West Virginia University Follow this and additional works at: https://researchrepository.wvu.edu/etd Recommended Citation Nayak, Ajay Padmaj, "Characterization of terrelysin, a potential biomarker for Aspergillus terreus" (2012). Graduate Theses, Dissertations, and Problem Reports. 3598. https://researchrepository.wvu.edu/etd/3598 This Dissertation is protected by copyright and/or related rights. It has been brought to you by the The Research Repository @ WVU with permission from the rights-holder(s). You are free to use this Dissertation in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you must obtain permission from the rights-holder(s) directly, unless additional rights are indicated by a Creative Commons license in the record and/ or on the work itself. This Dissertation has been accepted for inclusion in WVU Graduate Theses, Dissertations, and Problem Reports collection by an authorized administrator of The Research Repository @ WVU. For more information, please contact [email protected]. Characterization of terrelysin, a potential biomarker for Aspergillus terreus Ajay Padmaj Nayak Dissertation submitted to the School of Medicine at West Virginia University in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Immunology and Microbial Pathogenesis Donald H. Beezhold, -
Concomitant Mucormycosis with Aspergillosis in Patients with Uncontrolled Diabetes
DOI: 10.7860/JCDR/2021/47912.14507 Case Series Concomitant Mucormycosis with Aspergillosis in Patients with Uncontrolled Diabetes Microbiology Section Microbiology Mellitus: A Case Series ARPANA SINGH1, AROOP MOHANTY2, SHWETA JHA3, PRATIMA GUPTA4, NEELAM KAISTHA5 ABSTRACT Fungal infections are life threatening especially in presence of immunosuppression or uncontrolled diabetes mellitus mainly due to their invasive potential. Mucormycosis of the oculo-rhino-cerebral region is an opportunistic, aggressive, fatal and rapidly spreading infection caused by organisms belonging to Mucorales order and class Zygomycetes. The organisms associated are ubiquitous. Aspergillosis is a common clinical condition caused by the Aspergillus species, most often by Aspergillus fumigatus (A. fumigatus). Both fungi have a predilection for the immunosuppressive conditions, with uncontrolled diabetes and malignancy being the most common among them. Mucormycosis is caused by environmental spores which get access into the body through the lungs and cause various systemic manifestations like rhino-cerebral mucormycosis. Here, a case series of such concomitant infections of Aspergillus and Mucor spp from Rishikesh, Uttarakhand, India is reported. Keywords: Diabetes, Fungal infection, Invasive mycoses, Rhinosinusitis INTRODUCTION Case 2 Mucorales are the universally distributed saprophytes causing A 60-year-old female patient presented with complaints of aggressive and opportunistic infection. They are angio-invasive fever for three days and ptosis for one day. She was a known in nature. Aspergillosis is the clinical condition caused by the case of Type 2 Diabetes Mellitus (T2DM) and hypertension for Aspergillus species most often A. fumigatus [1]. It proves to be the past seven years but was on irregular drug metformin and fatal, if it infects secondarily to the brain. -
Allergic Bronchopulmonary Aspergillosis in a Patient with Chronic Obstructive Pulmonary Disease
Prim Care Respir J 2012; 21(1): 111-114 CASE-BASED LEARNING Allergic bronchopulmonary aspergillosis in a patient with chronic obstructive pulmonary disease Elias Mira,*Ashok Shaha a Department of Respiratory Medicine, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi, India Originally received 13th April 2011; resubmitted 27th July 2011; revised 16th September 2011; accepted 17th September 2011; online 5th January 2012 Summary Allergic bronchopulmonary aspergillosis (ABPA) is a debilitating lung disease which occurs as a result of interplay between a variety of host and environmental factors. It occurs in certain susceptible individuals who develop hypersensensitivity to the colonised Aspergillus species. ABPA is a complicating factor in 2% of patients with asthma and is also seen in patients with cystic fibrosis. Asthma and chronic obstructive pulmonary disease (COPD) are known to share key elements of pathogenesis. It is well known that ABPA can occur in patients with asthma, but it has recently been reported in patients with COPD as well. We report a 55-year-old male ex-smoker who presented with complaints of exertional breathlessness and productive cough for five years and an episode of haemoptysis four days prior to presentation. Spirometery showed airflow obstruction which was not reversible with bronchodilators. Chest CT scan revealed paraseptal emphysema along with central bronchiectasis (CB) in the right upper lobe and bilateral lower lobes. A type I skin hypersensitivity reaction to Aspergillus species was elicited. He fulfilled the serological criteria for ABPA and was diagnosed as having concomitant COPD and ABPA-CB. The patient was initiated on therapy for COPD along with oral corticosteroids, on which he had remarkable symptomatic improvement. -
Key Nutrients to Aspergillus Fumigatus Virulence
Journal of Fungi Review Nitrogen, Iron, and Zinc Acquisition: Key Nutrients to Aspergillus fumigatus Virulence Uxue Perez-Cuesta , Xabier Guruceaga , Saioa Cendon-Sanchez , Eduardo Pelegri-Martinez, Fernando L. Hernando, Andoni Ramirez-Garcia * , Ana Abad-Diaz-de-Cerio and Aitor Rementeria * Fungal and Bacterial Biomics Research Group, Department of Immunology, Microbiology, and Parasitology, University of the Basque Country (UPV/EHU), 48940 Leioa, Spain; [email protected] (U.P.-C.); [email protected] (X.G.); [email protected] (S.C.-S.); [email protected] (E.P.-M.); fl[email protected] (F.L.H.); [email protected] (A.A.-D.-d.-C.) * Correspondence: [email protected] (A.R.-G.); [email protected] (A.R.) Abstract: Aspergillus fumigatus is a ubiquitous soil decomposer and an opportunistic pathogen that is characterized by its large metabolic machinery for acquiring nutrients from media. Lately, an ever-increasing number of genes involved in fungal nutrition has been associated with its virulence. Of these, nitrogen, iron, and zinc metabolism-related genes are particularly noteworthy, since 78% of them have a direct implication in virulence. In this review, we describe the sensing, uptake and regulation process of the acquisition of these nutrients, the connections between pathways and the virulence-implicated genes. Nevertheless, only 40% of the genes mentioned in this review have been assayed for roles in virulence, leaving a wide field of knowledge that remains uncertain and might Citation: Perez-Cuesta, U.; offer new therapeutic and diagnostic targets. Guruceaga, X.; Cendon-Sanchez, S.; Pelegri-Martinez, E.; Hernando, F.L.; Keywords: Aspergillus fumigatus; nitrogen; iron; zinc; nutrient acquisition; siderophores; metals; Ramirez-Garcia, A.; transcription factors; primary metabolism; nitrogen metabolite repression Abad-Diaz-de-Cerio, A.; Rementeria, A. -
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PEARLS OF LABORATORY MEDICINE Hyaline and Mucorales Molds Dennise E. Otero Espinal MD Assistant Director of Clinical Pathology at Lenox Hill Hospital DOI: 10.15428/CCTC.2019.304881 © Clinical Chemistry Objectives • Name methods for mold identification • Describe the characteristics of hyaline molds • Discuss some of the most commonly Mucorales fungi isolated in the laboratory 2 Methods of Identification • Direct visualization • Slide prepared before setting fungal cultures • Calcofluor White fluorescent stain o Non- specific 1 Hyaline mold stained with Calcofluor White fluorescent stain. Photo credit: Melinda Wills • Histology o Hematoxilin & Eosin stain (H&E) o Gomori Methenamine Silver stain (GMS) o Periodic acid–Schiff (PAS) o Non-specific 2 Rhizopus spp. - H&E, 60x 3 Methods of Identification • Culture • Colony morphology • Lactophenol cotton blue stain • Media types: o Non-selective o With antibiotics o With cycloheximide • MALDI-TOF MS • DNA sequencing Penicillium spp., lactophenol cotton blue stain, 60x 4 Hyaline Molds • Rapid growth • Thin, regularly septate hyphae • Acute angle branching septations (blue arrows) • Aspergillus spp., Fusarium spp., and Scedosporium spp. may be indistinguishable by Hyaline mold, H&E stain, 60x histology 5 Hyaline Molds - Aspergillus fumigatus Complex • Most common cause of invasive aspergillosis, allergic aspergillosis, fungal sinusitis, and aspergilloma (fungus ball) 1 • Fast growing blue-green colonies with white borders (image 1), white to tan on reverse • Dome or flask-shaped vesicle with uniseriate phialides covering 2/3 of the vesicle (red arrows image 2) 2 A. fumigatus complex - Lactophenol cotton blue stain, 60x 6 Hyaline Molds - Aspergillus flavus Complex • Second most common cause of invasive aspergillosis • Producer of aflatoxin • Fast growing, yellow-green 1 colonies, yellowish on reverse (image 1) • Rough or spiny conidiophore, may be hard to see (red arrow image 2) • Globose vesicle covered by uniseriate or biseriate phialides 2 A. -
THE 10 BEST PLANTS to CLEAN YOUR INDOOR AIR the NASA Clean Air Study Identified the Top Plants You Can Use to Improve Your Indoor Air Quality
THE 10 BEST PLANTS TO CLEAN YOUR INDOOR AIR The NASA Clean Air Study identified the top plants you can use to improve your indoor air quality. It’s best to have about one potted plant for every 100 square feet. Many of the plants on NASA’s list are affordable and remove significant amounts of indoor pollutants that contribute to ‘sick building syndrome.’ Symptoms range from allergies, asthma, headaches and fatigue to nervous-system disorders, espiratory issues and chronic illnesses including cancer. The benefits expand beyond your home as well. Plants can create healthier air in hospital rooms, office cubicles, and hair or nail salons. The top ten plants for removing formaldehyde, benzene, and carbon monoxide from the air are: 1 ARECA PALM 5 ENGLISH IVY Also called the “living room plant”. Best suited for This climbing vine is great at removing benzene humid environments. It can reach 10 to 12 feet in and especially formaldehyde. Formaldehyde is a height. This plant was rated best for removing all common household pollutant found in floorboard, indoor air toxins tested. It removes co2 and converts insulation, MDF particleboard and wrinkle-free it into oxygen. or easy care draperies. It also removes benzene, trichloroethylene, xylene and toluene. 2 LADY PALM A tall, fanned-leaf plant, the Lady Palm removes 6 JANET CRAIG (DRACAENA) trichloroethylene, xylene, toluene and ammonia; The Dracaena grows to 10 feet tall. It’s easy to grow which is toxic to the respiratory system and found in and is best suited for bright indirect sunlight. cleaners, textiles, and dyes. -
Smart Plant Based Air Purifier Using Nodemcu
www.ijcrt.org © 2021 IJCRT | Volume 9, Issue 4 April 2021 | ISSN: 2320-2882 SMART PLANT BASED AIR PURIFIER USING NODEMCU 1PRANSHU TAPAN MANDAL, 2DANISH ILTEZA ANSARI, 3ANKIT AJAY NAGDEVE, 4 PROF. JUNAID MANDVIWALA, 5Dr. VARSHA SHAH. 123STUDENT, 4ASSISTANT PROFESSOR,5PRINCIPAL. “RIZVI COLLEGE OF ENGINEERING” (RCOE), MUMBAI UNIVERSITY, MUMBAI, INDIA. Abstract: The increase in pollution everywhere, due to increase in urbanization and industrialization, attracts the major concern of all the authority and the people at global level look forward to find out the way to resolve it. Pollutants are the substances that cause pollution and that affect the environment. Though the efforts were to control air pollution have traditionally focused on the outdoor air, it is now apparent that high concentrations of contaminants are common inside most private and public buildings (indoor air). The concerns about public health problems increase here due to indoor air pollution and are based on the fact that states, urban residents usually spend more than 90 percent of their time indoors, and the concentrations of some contaminants are higher in indoors than outdoors, and some of the pollutants personal exposures are not characterized effectively by outdoor measurements. Our idea is based on the NASA Clean Air Study. This idea is mainly focusing on reducing indoor air pollution through the use of plants. Index Terms - Clean Air Study, Volatile Organic Compounds, Air Pollution, Hydrocarbons, Humidity. I. INTRODUCTION In this idea, the leaves, roots, soil, and associated microorganisms of plants have been evaluated as a possible means of reducing indoor air pollutants. Additionally, a novel approach of using plant systems for removing high concentrations of indoor air pollutants such as cigarette smoke, organic solvents, and possibly radon has been designed from this work.