Improved Rootstocks for Imperial Mandarin Industry Impact: Proven Track Record
Improved rootstocks for Imperial mandarin Industry impact: proven track record
Prior to 2000…… Troyer and Cleopatra
Benton for Imperial 2003, Sunstate Experiment
Barkley for Imperial 2017, Bakers Experiment 13-years-old
Troyer: Benching & tree decline Cleopatra: Phytophthora challenge Troyer 1960s Benton 2003 Barkley 2017
Joint field-day: 7th March 2017, Gayndah
Organised by: QRAC, Citrus Australia Ltd Queensland Citrus Improvement Scheme Inc. Dept of Agriculture & Fisheries Queensland RSVP 3rd March (this Friday) “Growing to market requirements”
What’s that got do with rootstocks?
Funding: Voluntary Contribution project
CT13004 Queensland Citrus Improvement Scheme: finding better rootstocks for Australia. 1. Gayndah ‘Imperial’ rootstock experiment
2. CTV replication and movement in advanced rootstocks
3. Emerald ‘Imperial’ rootstock experiment
4. Breeding new P.trifoliata hybrids with CTV resistance
5. The commercial value of “Extreme hybrids” as rootstocks Laboratory and Nursery Trials: Citrus Tristeza Virus inoculation and screening: >2,500 hybrids Phytophthora screening: >1,500 hybrids
Field Trials: Tegege, Sunstate, 1998: 10 rootstocks→Benton Wallaville, McClennan 2000: 28 rootstocks Gayndah, Bakers, 2004: 33 rootstocks→Barkley Emerald, Presslers, 2011: 66 rootstocks Gayndah, Shepherds, 2013: 245 rootstocks Wallaville, Spencer Ranch, 2016: 138 rootstocks Mundubbera/Gayndah, MHP, 2016/17: 14 rootstocks
Why use Imperial scions?.....good ‘canary’ Rootstock objective: better fruit quality Rootstock objective: better fruit quality
But… Rootstock requirements for Australian conditions
Essential: tolerance to CTV resistance to phytophthora root and collar rot good horticultural performance
Desirable: salt tolerance lime tolerance resistance to nematodes tolerance to waterlogging control of tree size
Minor importance: tolerance to exocortis drought tolerance cold tolerance seedy and nucellar embryony
Australian National Citrus Rootstock Screening Working Party, 1985 Breeding for CTV resistance
Infected bud onto each hybrid Hybrid bud onto infected rootstock Better method, faster results
>800 hybrids completely resistant to CTV, carrying the resistance gene from Poncirus. New challenge…rootstock hybrids with 2 copies of the resistance gene → rapid field deployment.
Breeding for phytophthora resistance
Old Method
Cheaper New Method Faster Cleaner Breeding for salt tolerance Breeding for granulation resistance
Use field trials to identify potential parents (with low granulation) Hybridise these parents Cull for CTV, phytophthora, salt Assess for granulation Unproven method Rootstock requirements for Australian conditions
Essential: tolerance to CTV resistance to phytophthora root and collar rot good horticultural performance
Desirable: salt tolerance lime tolerance resistance to nematodes tolerance to waterlogging control of tree size
Minor importance: tolerance to exocortis drought tolerance cold tolerance seedy and nucellar embryony
Australian National Citrus Rootstock Screening Working Party, 1985 Rootstock requirements for Australian conditions
Essential: tolerance to CTV resistance to phytophthora root and collar rot good horticultural performance HLB tolerance
Desirable: salt tolerance lime tolerance resistance to nematodes tolerance to waterlogging control of tree size warm climate productivity
Minor importance: tolerance to exocortis drought tolerance cold tolerance seedy and nucellar embryony gall wasp
Breeding for new challenges
Choose the right parents (e.g. pumelo, Eremocitrus, Microcitrus for HLB tolerance) Maximise genetic diversity Build breeding populations that have the ‘right stuff’ and dump the rest
e.g. K15 x 14Q055 • 556 hybrid seedlings • 88% fail phytophthora screening • 66 hybrids remaining – 71% fail CTV screening – 19 hybrids remaining • ??% fail salt screening • perhaps ~10 hybrids for field rootstock testing
~98% of hybrids dumped within 2 years of sowing, and prior to field planting. Minimum cost and effort.
Emerald Imperial Rootstock Trial
• Planted August 2011 • 65 different rootstocks, 891 data trees • Detailed assessments 2014, 2015, 2016
• Major impacts on canopy size, productivity, quality
• Emergence of winners and losers
N639 X639 H639 Nelspruit
Don’t use it! Wallaville Imperial Rootstock Trial
Planted November 2016
605 trees
138 rootstocks (screened for CTV and phytophthora resistance prior to planting)
Genetic diversity Hybrids containing: Poncirus, Swinglea, Clymenia, Atalantia, Fortunella, Severinia, Citropsis, Hesperathusa, Citrus australasica, C.garrawayi, C.glauca, C.junos, C.wintersii, C.reticulata, C.maxima, C.aurantium.
The most genetically diverse rootstock experiment ever planted
Thankyou
• Funding: DAF Qld, Hort Innovation, Qld Citrus Improvement Scheme • Staff: Deb Gultzow, Toni Newman • Field-trial hosts: Bakers, Benhams, Berthelsens, Emmertons, Presslers, Shepherds, Spencer, Trotts. • Professional input and supplies: Andrew Miles and other private researchers/consultants. Helen Hofman, Graeme Sanderson, Nerida Donovan, Mark Skewes, Matt Weinert, Yu Pei Tan and other Agency colleagues. Pat Barkley, Andrew Harty and other industry professionals. John Owen-Turner and Committee Members QCIS. Tim Herrman and the AusCitrus Board • National/ International collaboration and germplasm: NSW DPI, Uni of California, Riverside, CREA Italy, IAC New Caledonia, Jeju Uni South Korea, Uni of Florida Lake Alfred, USDA Fort Pierce, USDA Riverside, Thankyou
• Funding: DAF Qld, Hort Innovation, Qld Citrus Improvement Scheme • Staff: Deb Gultzow, Toni Newman • Field-trial hosts: Bakers, Benhams, Berthelsens, Emmertons, Presslers, Shepherds, Spencer, Trotts. • Professional input and supplies: Andrew Miles and other private researchers/consultants. Helen Hofman, Graeme Sanderson, Nerida Donovan, Mark Skewes, Matt Weinert, Yu Pei Tan and other Agency colleagues. Pat Barkley, Andrew Harty and other industry professionals. John Owen-Turner and Committee Members QCIS. Tim Herrman and the AusCitrus Board • National/ International collaboration and germplasm: NSW DPI, Uni of California, Riverside, CREA Italy, IAC New Caledonia, Jeju Uni South Korea, Uni of Florida Lake Alfred, USDA Fort Pierce, USDA Riverside,
Australian citrus growers/nurseries/consultants: for wanting better rootstocks!