DOCSLIB.ORG

(12) United States Patent (10) Patent No.: US 8,597,395 B2 G00dwin (45) Date of Patent: Dec

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
(12) United States Patent (10) Patent No.: US 8,597,395 B2 G00dwin (45) Date of Patent: Dec US00859.7395 B2 (12) United States Patent (10) Patent No.: US 8,597,395 B2 G00dwin (45) Date of Patent: Dec. 3, 2013 (54) METHODS OF REDUCING PLANTABIOTIC 5,599,769 A 2f1997 Hacker et al. STRESS BY APPLYING ACOMPOSITION 5,665,671 A 9, 1997 Zanin 5,747,020 A 5/1998 Rutherford et al. COMPRISING LIGNINS, TANNINS, AND 5,849,320 A 12/1998 Turnbladet al. HYDROCARBONS 5,928,997 A 7/1999 Bauer et al. 5,951,978 A 9, 1999 Redkina (75) Inventor: Brian B. Goodwin, Collierville, TN 6,022,744 A 2/2000 Tetteroo et al. 6,077,505 A 6/2000 Parke et al. (US) 6,080,220 A 6/2000 Sequiet al. 6,080,319 A 6/2000 Alther (73) Assignee: Floratine Biosciences, Inc., Collierville, 6,083,877 A 7/2000 Kinnersley et al. TN (US) 6,090,750 A 7/2000 Chollet et al. 6,121,193 A 9/2000 Segaud et al. (*) Notice: Subject to any disclaimer, the term of this 6,199,318 B1 3/2001 Stewart et al. patent is extended or adjusted under 35 6,261,996 B1 7/2001 Kittich et al. 6,277,787 B1 8/2001 Malefytet al. U.S.C. 154(b) by 239 days. 6,372,008 B1 * 4/2002 Boote et al. ....................... T1.63 6,434,884 B1 8/2002 Hartung (21) Appl. No.: 12/892,564 6,453,608 B1 9/2002 Flanagan et al. 6,557,298 B2 5/2003 Obert et al. (22) Filed: Sep. 28, 2010 6,698,137 B2 3, 2004 Muhr 6,855,536 B2 2/2005 Loh et al. (65) Prior Publication Data 6,911.415 B1 6/2005 Ueland et al. 7,001,869 B2 2/2006 Johnson US 2011 FOOT881.6 A1 Mar. 31, 2011 7,003,914 B2 2/2006 Legro et al. 7,182,951 B1 2/2007 Balachander et al. 7,213,367 B2 5, 2007 Wertz et al. Related U.S. Application Data 7,393,678 B2 7/2008 Triplett et al. 7,510,590 B2 3/2009 Anaya-Olvera (60) Provisional application No. 61/246,453, filed on Sep. 7,687,434 B2 3/2010 De Billot et al. 28, 2009. 2002fOO95864 A1 7/2002 Obert et al. 2002/0134012 A1 9/2002 Ding et al. 2003/0044382 A1 3/2003 Selvig et al. (51) Int. Cl. 2003. O130 120 A1 7/2003 Ziemer et al. AOIN 25/02 (2006.01) 2003/0228.679 A1 12/2003 Smith et al. AOIN 27/00 (2006.01) AOIN 65/00 (2009.01) (Continued) CI2O I/68 (2006.01) AOIH 3/00 (2006.01) FOREIGN PATENT DOCUMENTS AOIH 3/04 (2006.01) CA 1328.238 4f1994 (52) U.S. Cl. CA 2056107 7, 1998 USPC .......... 71/64.1; 71/64.01:504/100: 536/23.1; 536/25.3: 530/500 (Continued) (58) Field of Classification Search OTHER PUBLICATIONS None See application file for complete search history. Schulze et al. Environment as stress factor: stress physiology of plants. Plant Ecology. Springer, 2005. pp. 7-11.* (56) References Cited U.S. PATENT DOCUMENTS (Continued) 2,200,532 A 12/1939 Sherman 3,958,016 A 5, 1976 Galle et al. Primary Examiner — Cathy Kingdon Worley 4,069,034 A 1/1978 Hoover Assistant Examiner — Ashley K Buran 4,245,432 A 1/1981 Dannelly 4,249,343 A 2/1981 Dannelly (74) Attorney, Agent, or Firm — Christopher J. Knors: 4,272,920 A 6, 1981 Dawson Moore & Van Allen PLLC 4,337,077 A 6, 1982 Rutherford 4,367,609 A 1/1983 Lloyd 4,698,090 A 10, 1987 Marihart 4,769,221 A 9, 1988 Marihart (57) ABSTRACT 4,786.307 A 11, 1988 Marihart 4,828,600 A 5, 1989 McCabe et al. A method of regulating plant genes is provided. The method 4,875,921 A 10, 1989 Paau provides improved drought stress or salinity stress for plants. 4,878,936 A 11, 1989 Handelsman et al. 5,026,416 A 6, 1991 Alexander The method comprises treating a part of a plant or the locus 5,044,116 A 9/1991 Gago et al. thereof with a composition of matter, the composition of 5,087.475 A 2f1992 Bazinet al. matter comprising an agriculturally acceptable mixture of 5,129, 180 A 7, 1992 Stewart compounds of natural organic material of defined composi 5,178,661. A * 1/1993 van der Watt et al. ............ T1/24 5,204,368 A 4/1993 Cronje et al. tion. 5,250,500 A 10, 1993 Jones et al. 5,300,127 A 4, 1994 Williams RE34,670 E 7, 1994 Williams et al. 21 Claims, 18 Drawing Sheets US 8,597,395 B2 Page 2 (56) References Cited Wershaw, “Evaluation of Conceptual methods of natural matter (Humus) from a a consideration of the chemical and biochemical U.S. PATENT DOCUMENTS processes of Humification.” 2004, US Dept. of the Interior, US geo logical Survey; Scientific Investigations Report 2004-5121; pp. 5.6, 2003/0228981 A1 12/2003 Wertz et al. and 11. 2004/OO77498 A1 4/2004 Lynch Machine English translation of JP 05-194951 (1993).* 2004/O118040 A1 6/2004 Asrar et al. Johnson et al. Genetic control of plant growth. New Phytologist. 2005/O1972.51 A1 9/2005 Ding et al. 2001, 191: 319-333. 2005/O1972.53 A1 9, 2005 Stoller et al. Zhang. Influence of plant growth regulators on turgrass growth, 2006, OO32120 A1 2/2006 McPherson antioxidant status, and drought tolerance. Dissertation. Virginia Poly 2006, OO32281 A1 2/2006 Meyer 2006/0229203 A1 10, 2006 Peltonen et al. technic Institute and State University. 1997.* 2007/0O393.65 A1 2/2007 King et al. Shandong Chuangxin Humic Acid Technology Co., Ltd. Humic Acid 2007.0068072 A1 3/2007 Xavier et al. + Amino Acid Powder. http://www.humicacidcorp.com/.2009. p. 1.* 2007/0074451 A1 4/2007 Pearce et al. Shandong Chuangxin Humic Acid Technology Co., Ltd. Nitro 2007/0212772 A1 9, 2007 Hill et al. Humic Acid. http://www.humicacidcorp.com/. 2009. pp. 1-2.* 2008.0004178 A1 1/2008 Ding et al. Korean Intellectual Property Office, PCT International Preliminary 2008/0242544 A1 10/2008 Duckham et al. Report on Patentability and Written Opinion of the International 2008/0274885 A1 11/2008 Martin et al. Searching Authority for International Application No. PCT/US2010, 2009/0105076 A1 4/2009 Stewart et al. 050520, dated Apr. 3, 2012. 2009/0105077 A1 4/2009 Bhatti et al. Korean Intellectual Property Office, PCTInternational Search Report 2010, OO16162 A1 1/2010 Goodwin and Written Opinion of the International Searching Authority for 2011/0053771 A1 3/2011 Goodwin 2011/0077155 A1 3/2011 Goodwin International Application No. PCT/US2010/050520, dated Jun. 22, 2012/001.5805 A1 1/2012 Goodwin ...................... 504,100 2011. 2012fO196747 A1* 8, 2012 Goodwin .... 504,100 Wershaw, Robert L., “Evaluation of Conceptual Models of Natural 2013,0005570 A1 1/2013 Goodwin ...................... 504,101 Organic Matter (Humus) From a Consideration of the Chemical and Biochemical Processes of Humification'. Scientific Investigations FOREIGN PATENT DOCUMENTS Report 2004-5121. US Department of the Interior, US Geological Survey (2004). EP 164908 9, 1989 Pandey, Girdhar, et al., “ABR1, an APETALA2-Domain Transcrip EP 560943 3, 1999 tion Factor that Functions as a Repressor of ABA Response in EP 949.975 10, 2002 Arabidopsis'. Plant Physiology, vol. 139, No.3, pp. 1185-1193 (Nov. EP 1464635 A1 10, 2004 2005). EP 1238714 3, 2005 http://ihss.gatech.edu/ihss2/whatarehs.html. What are Humic Sub JP 05-194951 * 8, 1993 ............. CO9K 17 OO stances? (Dec. 2007). WO WO90 13420 11, 1990 http://ihss.gatech.edu/ihss2/sources.html, Source Materials for IHSS WO WO9015138 12/1990 Samples (Aug. 1, 2009). WO WO9210081 6, 1992 Landec AG Inc.—Seeds of Innovation, IntelliCoat Early Plant Corn, WO WO9517806 7, 1995 Reference Guide. WO WOO3O20O28 3, 2003 WO WOO3O2O837 3, 2003 Chinese Patent Office, Chinese Patent Application No. WO WOO3094,614 11, 2003 2013070300854820 Office Action dated Jul. 8, 2013, pp. 1-8. WO WO2007O24753 3, 2007 European Patent Office, European Patent Application No. WO WO2007 143791 12/2007 10819641.1 Extended European Search Reportdated Jul. 8, 2013. pp. WO WO2O09068195 6, 2009 1-6. WO WO2O09068213 6, 2009 Collaberative, “Humus'. Wikipedia, Internet Article, Jan. 1, 2002, URL: http://en.wikipedia.org/wiki/Humus, retreived on Jun. 24. OTHER PUBLICATIONS 2013, p. 1. Steinberg, et al., “Humic Substances, Part 2: Interactions with Organ Jonak et al. Stress signaling in plants: a mitogen-activated protein isms”, Environ Sci Pollut Res Int (2008) 15(2), pp. 128-235. kinase pathway is activated by cold and drought. PNAS. 1996. 93: 11274-11279.* * cited by examiner U.S. Patent Dec. 3, 2013 Sheet 1 of 18 US 8,597,395 B2 C CONTRO FIG. 1 U.S. Patent Dec. 3, 2013 Sheet 2 of 18 US 8,597,395 B2 s U.S. Patent Dec. 3, 2013 Sheet 3 of 18 US 8,597,395 B2 C CONTRO FIG. 3 U.S. Patent Dec. 3, 2013 Sheet 4 of 18 US 8,597,395 B2 Control FIG. 4 U.S. Patent Dec. 3, 2013 Sheet 5 of 18 US 8,597,395 B2 CONTRO FIG. 5 U.S. Patent Dec. 3, 2013 Sheet 6 of 18 US 8,597,395 B2 U.S. Patent Dec. 3, 2013 Sheet 7 of 18 US 8,597,395 B2 ********** 88:38: FIG. 7 U.S. Patent Dec. 3, 2013 Sheet 8 of 18 US 8,597,395 B2 3. 3. : 8883:38: FIG. 8 U.S.
Load more

Recommended publications
  • Hormonal Regulation During Initial Berry Development in Grapevine
    1 Année 2013 Thèse N° 2064 THESE pour le DOCTORAT DE L’UNIVERSITE BORDEAUX 2 Ecole Doctorale des Sciences de la Vie et de la Santé de l’Université Bordeaux-Segalen Spécialité: Biologie Végétale Hormonal regulation during initial berry development in grapevine par María Francisca Godoy Santin Née le 20 Février 1985 à Santiago, Chili Soutenue publiquement le 15 Novembre, à Pontificia Universidad Católica de Chile, Santiago, Chili Membres du Jury : Pr. Alexis KALERGIS, Universidad Católica de Chile Pr. Michael HANDFORD, University of Chile Rapporteur Pr. Claudia STANGE, University of Chile Rapportrice Pr. Loreto HOLUIGUE, Universidad Católica de Chile Examinatrice Pr. Serge DELROT, Université Victor Ségalen Bordeaux2 Examinateur Pr. Patricio ARCE-JONHSON, Universidad Católica de Chile Co-Directeur de thèse Dr. Virginie LAUVERGEAT, Université Bordeaux1 Co-Directrice de thèse 2 Acknowledgements I would like to thank my advisors Dr Patricio Arce and Dr Virginie Lauvergeat for their guidance, patience and help at every step of the way. I also want to specially acknowledge to all my labmates for their ideas, support, laughs and much more: Jenn, Xime, Amparo, Mindy, Anita, Felipe, Tomás, Susan, Mónica, Jessy, Claudia, Dani H, and everyone else who made the working place the best one ever. Special thanks to Consuelo, who guided me from the first day, and to Anibal, who has been an amazing help during these lasts years. I want to thank Nathalie Kühn for all her ideas, discussion, company and help during these experiments, where she played a fundamental part. Also, I am very grateful to my other lab across the sea, in INRA, for receiving me there: Mariam, María José, Julien, Eric, Pierre, Le, Huan, Messa, David, Fatma and all the staff for all their help.
    [Show full text]
  • The Metabolism of Androstenone and Other Steroid Hormone Conjugates in Relation to Boar Taint
    The Metabolism of Androstenone and Other Steroid Hormone Conjugates in Relation to Boar Taint by Heidi M. Laderoute A Thesis presented to The University of Guelph In partial fulfillment of requirements for the degree of Master of Science in Animal and Poultry Science with Toxicology Guelph, Ontario, Canada © Heidi M. Laderoute, April, 2015 ABSTRACT THE METABOLISM OF ANDROSTENONE AND OTHER STEROID HORMONE CONJUGATES IN RELATION TO BOAR TAINT Heidi M. Laderoute Advisor: University of Guelph, 2015 Dr. E.J. Squires Increased public interest in the welfare of pigs reared for pork production has led to an increased effort in finding new approaches for controlling the unpleasant odour and flavour from heated pork products known as boar taint. Therefore, this study investigated the metabolism of androstenone and the enzymes involved in its sulfoconjugation in order to further understand the pathways and genes involved in the development of this meat quality defect. Leydig cells that were incubated with androstenone produced 3-keto- sulfoxy-androstenone, providing direct evidence, for the first time, that sulfoconjugation of this steroid does occur in the boar. In addition, human embryonic kidney cells that were overexpressed with porcine sulfotransferase (SULT) enzymes showed that SULT2A1, but not SULT2B1, was responsible for sulfoconjugating androstenone. These findings emphasize the importance of conjugation in steroid metabolism and its relevance to boar taint is discussed. ACKNOWLEDGEMENTS I would like to gratefully and sincerely thank my advisor, Dr. E. James Squires, for providing me with the opportunity to be a graduate student and for introducing me to the world of boar taint. This project would not have been possible without your guidance, encouragement, and patience over the last few years.
    [Show full text]
  • Intratumoral Estrogen Sulfotransferase Induction Contributes to the Anti-Breast Cancer Effects of the Dithiocarbamate Derivative TM208
    npg Acta Pharmacologica Sinica (2015) 36: 1246–1255 © 2015 CPS and SIMM All rights reserved 1671-4083/15 www.nature.com/aps Original Article Intratumoral estrogen sulfotransferase induction contributes to the anti-breast cancer effects of the dithiocarbamate derivative TM208 Xi-wei JI 1, 2, Guang-ping CHEN4, Yan SONG5, Ming HUA1, Li-jie WANG1, Liang LI1, Yin YUAN1, Si-yuan WANG1, Tian-yan ZHOU1, 3, *, Wei LU1, 3 1Department of Pharmaceutics, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China; 2Institute of Clinical Pharmacology, Peking University First Hospital, Beijing 100191, China; 3State Key Laboratory of Natural and Biomimetic Drugs, Peking University, Beijing 100191, China; 4Department of Physiological Sciences, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK 74078, USA; 5Department of Molecular and Cellular Pharmacology, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China Aim: Sulfotransferase-catalyzed sulfation is the most important pathway for inactivating estrogens. Thus, activation of estrogen sulfotransferase (EST) may be an alternative approach for the treatment of estrogen-dependent breast cancer. In this study we investigated the involvement of EST in anti-breast cancer effects of the dithiocarbamate derivative TM208 in vitro and in vivo. Methods: The viability of human breast cancer MCF-7 cells was determined using a SBB assay. Nude mice bearing MCF-7 cells were orally administered TM208 (50 and 150 mg·kg-1·d-1) for 18 days. The xenograft tumors and uteri were collected. The mRNA expression of EST was examined with real-time PCR. EST protein was detected with Western blot, ELISA or immunohistochemical staining assays. A radioactive assay was used to measure the EST activity.
    [Show full text]
  • The Role of Estrogen Sulfotransferase in Ischemic Acute Kidney Injury
    Title Page The role of estrogen sulfotransferase in ischemic acute kidney injury By Anne Caroline Silva Barbosa Bachelor of Pharmacy, UFRN, 2015 Submitted to the Graduate Faculty of the School of Pharmacy in partial fulfillment of the requirements for the degree of Doctor of Philosophy University of Pittsburgh 2020 i Committee Membership Page UNIVERSITY OF PITTSBURGH SCHOOL OF PHARMACY This dissertation was presented By Anne Caroline Silva Barbosa It was defended on July 17, 2020 And approved by Christian Fernandez, PhD, Assistant Professor, Pharmaceutical Sciences Robert B. Gibbs, PhD, Professor, Pharmaceutical Sciences Youhua Liu, MD, PhD, Professor, Pathology Thomas D. Nolin, PhD, Associate Professor, Pharmacy & Therapeutics Dissertation Advisor: Wen Xie, MD, PhD, Professor, Pharmaceutical Sciences ii Copyright © by Anne Caroline Silva Barbosa 2020 iii Abstract The role of estrogen sulfotransferase in ischemic acute kidney injury Anne Caroline Silva Barbosa, PhD University of Pittsburgh, 2020 Acute kidney injury (AKI) is a sudden impairment of kidney function. It has been suggested that estrogens may protect mice from AKI. Estrogen sulfotransferase (SULT1E1/EST) plays an important role in estrogen homeostasis by sulfonating and deactivating estrogens, but the role of SULT1E1 in AKI has not been previously reported. In this dissertation, Wild-type (WT) mice, Sult1e1 knockout (Sult1e1 KO) mice, and Sult1e1 KO mice with hepatic reconstitution of Sult1e1 were subjected to a bilateral kidney ischemia-reperfusion model of AKI, in the absence or presence of gonadectomy. Kidney injury was assessed at biochemical, histological and gene expression levels. WT mice treated with the Sult1e1 inhibitor triclosan were also used to determine the effect of pharmacological inhibition of Sult1e1 on AKI.
    [Show full text]
  • 12) United States Patent (10
    US007635572B2 (12) UnitedO States Patent (10) Patent No.: US 7,635,572 B2 Zhou et al. (45) Date of Patent: Dec. 22, 2009 (54) METHODS FOR CONDUCTING ASSAYS FOR 5,506,121 A 4/1996 Skerra et al. ENZYME ACTIVITY ON PROTEIN 5,510,270 A 4/1996 Fodor et al. MICROARRAYS 5,512,492 A 4/1996 Herron et al. 5,516,635 A 5/1996 Ekins et al. (75) Inventors: Fang X. Zhou, New Haven, CT (US); 5,532,128 A 7/1996 Eggers Barry Schweitzer, Cheshire, CT (US) 5,538,897 A 7/1996 Yates, III et al. s s 5,541,070 A 7/1996 Kauvar (73) Assignee: Life Technologies Corporation, .. S.E. al Carlsbad, CA (US) 5,585,069 A 12/1996 Zanzucchi et al. 5,585,639 A 12/1996 Dorsel et al. (*) Notice: Subject to any disclaimer, the term of this 5,593,838 A 1/1997 Zanzucchi et al. patent is extended or adjusted under 35 5,605,662 A 2f1997 Heller et al. U.S.C. 154(b) by 0 days. 5,620,850 A 4/1997 Bamdad et al. 5,624,711 A 4/1997 Sundberg et al. (21) Appl. No.: 10/865,431 5,627,369 A 5/1997 Vestal et al. 5,629,213 A 5/1997 Kornguth et al. (22) Filed: Jun. 9, 2004 (Continued) (65) Prior Publication Data FOREIGN PATENT DOCUMENTS US 2005/O118665 A1 Jun. 2, 2005 EP 596421 10, 1993 EP 0619321 12/1994 (51) Int. Cl. EP O664452 7, 1995 CI2O 1/50 (2006.01) EP O818467 1, 1998 (52) U.S.
    [Show full text]
  • Obesity-Induced Diabetes (Diabesity) in C57BL/Ksj Mice Produces Aberrant Trans-Regulation of Sex Steroid Sulfotransferase Genes
    Obesity-induced diabetes (diabesity) in C57BL/KsJ mice produces aberrant trans-regulation of sex steroid sulfotransferase genes. E H Leiter, H D Chapman J Clin Invest. 1994;93(5):2007-2013. https://doi.org/10.1172/JCI117194. Research Article The diabetes (db) gene is a recessive obesity mutation in the mouse capable of producing diabetes only through interaction with heretofore undefined modifiers in the genetic background of certain inbred strains. Here we identify the genetic map locations of androgen and estrogen sulfotransferase genes important in maintaining the balance of active sex steroids in the liver. The Std locus encoding dehydroepiandrosterone sulfotransferase was mapped to proximal Chromosome 7, and the Ste locus encoding estrogen sulfotransferase was mapped to Chromosome 5. The db mutation in the diabetes-susceptible C57BL/KsJ strain aberrantly regulated mRNA transcript levels from these two loci. Hepatic Ste mRNA transcripts were increased from undetectable levels in normal males and females to high levels in db/db mice of both sexes. An anomalous suppression of Std transcription was observed in db/db females, but not in normal females. These reciprocal changes in mRNA concentrations in mutant females were reflected by an induction of a high affinity estrogen sulfotransferase activity and a concomitant loss of dehydroepiandrosterone sulfotransferase activity. These db gene-elicited effects were specific for the sex steroid sulfotransferases since other potential sex steroid metabolizing enzymes (phenol sulfotransferase, sex steroid sulfohydrolase, and UDP-glucuronyltransferase) were unaffected. These aberrant changes would virilize hepatic metabolism in females by increasing the ratio of active androgens to estrogens. In human females, non-insulin-dependent diabetes mellitus often develops when visceral […] Find the latest version: https://jci.me/117194/pdf Obesity-induced Diabetes (Diabesity) in C57BL/KsJ Mice Produces Aberrant trans-Regulation of Sex Steroid Sulfotransferase Genes Edward H.
    [Show full text]
  • Table S1 List of Genes A) List of Genes Up-Regulated at Least Three-Fold in 15-16 DAA Immature Seeds of Ex1/Ex2 Vs Wt
    Table S1 List of genes A) List of genes up-regulated at least three-fold in 15-16 DAA immature seeds of ex1/ex2 vs wt Up (74) ex1ex2 vs wt Gene ID Common Description Localization Fold (+) At2g26150 ATHSFA2 heat shock transcription factor family protein nucleus 3.01 At4g01250 WRKY22 member of WRKY Transcription Factor; Group II-e nucleus 4.24 At1g68670 myb family transcription factor nucleus 5.64 a member of the ERF subfamily B-3 of ERF/AP2 transcription At4g34410 nucleus 11.02 factor a member of the ERF subfamily B-2 of ERF/AP2 transcription At2g47520 nucleus 4.72 factor a member of the ERF subfamily B-3 of ERF/AP2 transcription At4g17490 ATERF6 nucleus 4.99 factor At3g54810 zinc finger (GATA type) family protein, nucleus 4.98 At1g32640 ATMYC2 Basic helix-loop-helix (bHLH) protein (RAP-1) nucleus 3.62 a member of the DREB subfamily A-5 of ERF/AP2 transcription At1g19210 nucleus 17.89 factor At5g59820 RHL41 Encodes a zinc finger protein nucleus 4.55 At2g18160 GBF5 bZIP transcription factor family protein nucleus 5.25 At2g38470 WRKY33 putative WRKY-type DNA binding protein nucleus 17.47 At3g55980 zinc finger (CCCH-type) family protein, unknown 4.70 At3g10040 Expressed protein chloroplast 21.97 At4g10270 wound-responsive family protein chloroplast 8.68 At3g19680 Expressed protein chloroplast 3.63 At3g13310 DNAJ heat shock N-terminal domain-containing protein chloroplast 9.48 At3g52760 integral membrane Yip1 family protein, Yip1 domain chloroplast 3.11 At4g27280 calcium-binding EF hand family protein chloroplast 3.81 At5g26030 ferrochelatase-I
    [Show full text]
  • View PDF Version
    RSC Advances View Article Online PAPER View Journal | View Issue Structure-based design of nucleoside-derived analogues as sulfotransferase inhibitors† Cite this: RSC Adv.,2019,9,32165 Neil M. Kershaw, *a Dominic P. Byrne,b Hollie Parsons,a Neil G. Berry,a David G. Fernig,b Patrick A. Eyers b and Richard Cossticka Sulfotransferases (STs) catalyse the transfer of a sulfonyl group (‘sulfation’) from the enzyme co-factor 30- phosphoadenosine 50-phosphosulfate (PAPS) to a variety of biomolecules. Tyrosine sulfation of proteins and carbohydrate sulfation play a crucial role in many protein–protein interactions and cell signalling pathways in the extracellular matrix. This is catalysed by several membrane-bound STs, including tyrosylprotein sulfotransferase 1 (TPST1) and heparan sulfate 2-O-sulfotransferase (HS2ST1). Recently, involvement of these enzymes and their post-translational modifications in a growing number of disease areas has been reported, including inflammation, cancer and Alzheimer's disease. Despite their growing importance, the development of small molecules to probe the biological effect of TPST and carbohydrate ST inhibition remains in its infancy. We have used a structure-based approach and Creative Commons Attribution 3.0 Unported Licence. molecular docking to design a library of adenosine 30,50-diphosphate (PAP) and PAPS mimetics based Received 18th August 2019 upon 20-deoxyadenosine and using 20-deoxy-PAP as a benchmark. The use of allyl groups as masked Accepted 3rd October 2019 methyl esters was exploited in the synthesis of PAP-mimetics, and click chemistry was employed for the DOI: 10.1039/c9ra07567d divergent synthesis of a series of PAPS-mimetics. A suite of in vitro assays employing TPST1 and HS2ST, rsc.li/rsc-advances and a kinase counter screen, were used to evaluate inhibitory parameters and relative specificity for the STs.
    [Show full text]
  • All Enzymes in BRENDA™ the Comprehensive Enzyme Information System
    All enzymes in BRENDA™ The Comprehensive Enzyme Information System http://www.brenda-enzymes.org/index.php4?page=information/all_enzymes.php4 1.1.1.1 alcohol dehydrogenase 1.1.1.B1 D-arabitol-phosphate dehydrogenase 1.1.1.2 alcohol dehydrogenase (NADP+) 1.1.1.B3 (S)-specific secondary alcohol dehydrogenase 1.1.1.3 homoserine dehydrogenase 1.1.1.B4 (R)-specific secondary alcohol dehydrogenase 1.1.1.4 (R,R)-butanediol dehydrogenase 1.1.1.5 acetoin dehydrogenase 1.1.1.B5 NADP-retinol dehydrogenase 1.1.1.6 glycerol dehydrogenase 1.1.1.7 propanediol-phosphate dehydrogenase 1.1.1.8 glycerol-3-phosphate dehydrogenase (NAD+) 1.1.1.9 D-xylulose reductase 1.1.1.10 L-xylulose reductase 1.1.1.11 D-arabinitol 4-dehydrogenase 1.1.1.12 L-arabinitol 4-dehydrogenase 1.1.1.13 L-arabinitol 2-dehydrogenase 1.1.1.14 L-iditol 2-dehydrogenase 1.1.1.15 D-iditol 2-dehydrogenase 1.1.1.16 galactitol 2-dehydrogenase 1.1.1.17 mannitol-1-phosphate 5-dehydrogenase 1.1.1.18 inositol 2-dehydrogenase 1.1.1.19 glucuronate reductase 1.1.1.20 glucuronolactone reductase 1.1.1.21 aldehyde reductase 1.1.1.22 UDP-glucose 6-dehydrogenase 1.1.1.23 histidinol dehydrogenase 1.1.1.24 quinate dehydrogenase 1.1.1.25 shikimate dehydrogenase 1.1.1.26 glyoxylate reductase 1.1.1.27 L-lactate dehydrogenase 1.1.1.28 D-lactate dehydrogenase 1.1.1.29 glycerate dehydrogenase 1.1.1.30 3-hydroxybutyrate dehydrogenase 1.1.1.31 3-hydroxyisobutyrate dehydrogenase 1.1.1.32 mevaldate reductase 1.1.1.33 mevaldate reductase (NADPH) 1.1.1.34 hydroxymethylglutaryl-CoA reductase (NADPH) 1.1.1.35 3-hydroxyacyl-CoA
    [Show full text]
  • Xenobiotic Metabolism, Disposition, and Regulation by Receptors: from Biochemical Phenomenon to Predictors of Major Toxicities
    TOXICOLOGICAL SCIENCES 120(S1), S49–S75 (2011) doi:10.1093/toxsci/kfq338 Advance Access publication November 8, 2010 Xenobiotic Metabolism, Disposition, and Regulation by Receptors: From Biochemical Phenomenon to Predictors of Major Toxicities Curtis J. Omiecinski,1 John P. Vanden Heuvel, Gary H. Perdew, and Jeffrey M. Peters Department of Veterinary and Biomedical Sciences, Center for Molecular Toxicology and Carcinogenesis, Penn State University, University Park, Pennsylvania 16802 1To whom correspondence should be addressed at Department of Veterinary and Biomedical Sciences, Center for Molecular Toxicology and Carcinogenesis, Downloaded from 101 Life Sciences Building, Penn State University, University Park, PA 16802. Fax: (814) 863-1696. E-mail: [email protected]. Received September 1, 2010; accepted November 1, 2010 processes. Indeed, in the past 50 years, much has been gleaned To commemorate the 50th anniversary of the Society of regarding our mechanistic understanding of chemical toxicities toxsci.oxfordjournals.org Toxicology, this special edition article reviews the history and contributed by these coordinate biotransformation systems. current scope of xenobiotic metabolism and transport, with special From the early discoveries of these pathways, our scientific emphasis on the discoveries and impact of selected ‘‘xenobiotic receptors.’’ This overall research realm has witnessed dynamic perspective has witnessed extraordinary advances led by the development in the past 50 years, and several of the key milestone availability of new
    [Show full text]
  • Specific Estrogen Sulfotransferase (SULT1E1) Substrates and Molecular Imaging Probe Candidates
    Specific estrogen sulfotransferase (SULT1E1) substrates and molecular imaging probe candidates Graham B. Colea, Gyochang Keuma, Jie Liua, Gary W. Smallb, Nagichettiar Satyamurthya, Vladimir Kepea, and Jorge R. Barrioa,1 aDepartments of Molecular and Medical Pharmacology and bPsychiatry and Biobehavioral Sciences, David Geffen School of Medicine at University of California, Los Angeles, CA 90095-6948 Communicated by Michael E. Phelps, University of California, Los Angeles, Los Angeles, CA, December 28, 2009 (received for review July 24, 2009) This work focuses on the development of specific substrates for estrogen sulfotransferase (SULT1E1) to produce molecular imaging probes for this enzyme. SULT1E1 is a key enzyme in estrogen homeostasis, playing a central role in the prevention and develop- ment of human disease. In vitro sulfation assays showed alkyl and aryl substitutions to a fused heterocyclic system modeled after β- naphthol (βN), based on compounds that interact with the estrogen receptor, rendered several molecules with enhanced specificity for SULT1E1 over SULT1A1*1, SULT1A1*2, SULT1A3, and SULT2A1. Sev- eral 6-hydroxy-2-arylbenzothiazoles tested demonstrated excel- —V ∕K — K lent affinity max m ratios and specificity for SULT1E1. m values ranged from 0.12–2.36 μM. A strong correlation was ob- served between polarity of the 4′-sustituent on the 2-aryl moiety σ ðV ∕K Þ r ¼ 0 964 Fig. 1. (A) Interplay of estrogen sulfation and estrogen receptor (ER). Tissue (Hammett p) and the log max m ( . ). Substrate sensitiv- levels of E2 are regulated by SULT1E1 and STS, thus, modulating E2 interac- ity is influenced by the acidity of the 6-phenolic group tion with the estrogen receptor (ER).
    [Show full text]
  • (12) Patent Application Publication (10) Pub. No.: US 2015/0240226A1 Mathur Et Al
    US 20150240226A1 (19) United States (12) Patent Application Publication (10) Pub. No.: US 2015/0240226A1 Mathur et al. (43) Pub. Date: Aug. 27, 2015 (54) NUCLEICACIDS AND PROTEINS AND CI2N 9/16 (2006.01) METHODS FOR MAKING AND USING THEMI CI2N 9/02 (2006.01) CI2N 9/78 (2006.01) (71) Applicant: BP Corporation North America Inc., CI2N 9/12 (2006.01) Naperville, IL (US) CI2N 9/24 (2006.01) CI2O 1/02 (2006.01) (72) Inventors: Eric J. Mathur, San Diego, CA (US); CI2N 9/42 (2006.01) Cathy Chang, San Marcos, CA (US) (52) U.S. Cl. CPC. CI2N 9/88 (2013.01); C12O 1/02 (2013.01); (21) Appl. No.: 14/630,006 CI2O I/04 (2013.01): CI2N 9/80 (2013.01); CI2N 9/241.1 (2013.01); C12N 9/0065 (22) Filed: Feb. 24, 2015 (2013.01); C12N 9/2437 (2013.01); C12N 9/14 Related U.S. Application Data (2013.01); C12N 9/16 (2013.01); C12N 9/0061 (2013.01); C12N 9/78 (2013.01); C12N 9/0071 (62) Division of application No. 13/400,365, filed on Feb. (2013.01); C12N 9/1241 (2013.01): CI2N 20, 2012, now Pat. No. 8,962,800, which is a division 9/2482 (2013.01); C07K 2/00 (2013.01); C12Y of application No. 1 1/817,403, filed on May 7, 2008, 305/01004 (2013.01); C12Y 1 1 1/01016 now Pat. No. 8,119,385, filed as application No. PCT/ (2013.01); C12Y302/01004 (2013.01); C12Y US2006/007642 on Mar. 3, 2006.
    [Show full text]