Curriculum vitae

Date Prepared: October 11, 2018

Name: Daniel J. Siegwart, Ph.D. Office Address: Simmons Comprehensive Cancer Center Department of Biochemistry 6001 Forest Park Road, ND2.210E Dallas, TX 75390-8807 Work Phone: 214-645-6354 Work E-Mail: [email protected] Work Fax: 214-645-6347 Place of Birth: Pittsburgh, PA

Education

Degree Field of Study Year Institution (Honors) (Thesis advisor for PhDs) 2003 B.S. Biochemistry Lehigh University Chemistry 2005 M.S. Carnegie Mellon University (Krzysztof Matyjaszewski) Chemistry 2008 Ph.D. Carnegie Mellon University (Krzysztof Matyjaszewski)

Postdoctoral Training

Specialty/Discipline Year(s) Titles Institution (Lab PI for postdoc research) Postdoctoral Chemical Engineering, Cancer Massachusetts Institute of 2008 – 2012 Fellow (Robert Langer) Technology

Current Licensure and Certification

Honors and Awards

Year Name of Honor/Award Awarding Organization

Chemistry Department Research 2003 Lehigh University Honors The Joseph A. Solomon Memorial 2005 – 2008 Carnegie Mellon University Fellowship in Chemistry

1 East Asia and Pacific Summer 2006 National Science Foundation (NSF) Institutes (EAPSI) Fellowship Ruth L. Kirschstein National 2010 – 2012 Research Service Award (NRSA) National Institutes of Health (NIH) F32 Postdoctoral Fellowship Cancer Prevention and Research Institute of Texas 2012 – 2016 CPRIT Scholar Award (CPRIT) 2014 Award in Cancer Research Friends of the Comprehensive Cancer Center 2017 Research Scholar American Cancer Society 2018 Young Innovator Award in 2018 Editorial Board of Nano Research Nanobiotechnology

Faculty Academic Appointments

Year(s) Academic Title Department Academic Institution Simmons Comprehensive Cancer Center (primary); 2012 – 2018 Assistant Professor UT Southwestern Medical Center Department of Biochemistry (secondary) Simmons Comprehensive Associate Professor Cancer Center (primary); 2018 – UT Southwestern Medical Center (with tenure) Department of Biochemistry (secondary)

Appointments at Hospitals/Affiliated Institutions

Other Professional Positions

Year(s) Position Title Institution Eli Lilly and Company 2002 Intern Indianapolis, IN Research Fellow University of Tokyo 2006 Department of Materials Engineering Tokyo, Japan InVivo Therapeutics 2010 – 2013 Consultant Cambridge, MA Mirna Therapeutics 2014 – 2016 Consultant Austin, TX ReCode Therapeutics 2015 Co-founder Dallas, TX ReCode Therapeutics 2017 – Present Consultant Dallas, TX 2017 Consultant Back Bay Life Sciences 2017 Consultant Schlesinger Associates Consulting 2017 Consultant Boston Consulting Group 2 2018 Consultant AlphaSights

Major Administrative/Leadership Positions

Committee Service

Year(s) Name of Committee Institution/Organization UTSW Faculty level committees Chemical Safety Advisory Subcommittee 2013 – Present UTSW (CSAS) Committee on Graduate School Awards Representative of the Organic Chemistry 2016 – Present Graduate Program UTSW (Ida M. Green Award, Kirpatrick Endowment, Nominata Award, etc.) Review Committee 2017 – Present Brown-Goldstein Excellence Award for Postdoc UTSW Research

Student education level committees Participated in attainment and proposal exams UTSW Cancer Biology Program 2012 – Present (various) UTSW Organic Chemistry Program Student Thesis Committee (Chair): 2013 – 2016 UTSW Organic Chemistry Program Yang Li (PI: Jinming Gao) Qualifying Exam Committee (Chair): 2015 UTSW Organic Chemistry Program Aaron Coffin (PI: Joseph Ready) Student Thesis Committee (Member): UTSW Biological Chemistry 2015 – 2017 Austin Gay (PI: Arun Radhakrishnan) Program Student Thesis Committee (Chair): 2015 – 2018 UTSW Cancer Biology Program Brandt Nichols (PI: Angelique Whitehurst) Student Thesis Committee (Member): 2015 – Present UTSW Cancer Biology Program Ning Zhang (PIs: Jerry Shay, Gaudenz Danuser) Student Thesis Committee (Member): UTSW Biological Chemistry 2017 – Present Anam Shaikh (PI: John MacMillen) Program

Professional Societies

Dates Society Name, member American Chemical Society (ACS) Society for Biomaterials (SFB)

American Institute of Chemical Engineers (AIChE) Controlled Release Society (CRS)

3 Committees 2013 – 2015 Society for Biomaterials Nanomaterials Special Interest Group (SIG), Treasurer 2015 – 2017 Society for Biomaterials Nanomaterials Special Interest Group (SIG), Chair 2017 – Present Society for Biomaterials Nanomaterials Special Interest Group (SIG), Vice Chair ACS Chemical & Engineering News (C&EN) Selection Committee for the “2017 2017 Talented 12” Founding Chair of the Board, Controlled Release Society, Gene Delivery and Editing 2018 Focus Group

Grant Review Activities

Year(s) Name of Review Committee Organization Biotechnology and Biological 2014 Grant Peer Review Committee Sciences Research Council (BBSRC), United Kingdom Royal College of Surgeons in Ireland 2014 Reviewer Office of Research and Innovation Agency for Science, Technology and 2014 Joint Council Office (JCO) Research (A*STAR), Singapore 2015, 2016, Petroleum Research Foundation (PRF) American Chemical Society (ACS) 2017 2015 Reviewer Ohio Cancer Research Associates 2016 Review Committee Mary Kay Foundation Natural Sciences and Engineering 2017 Reviewer Research Council of Canada (NSERC) 2017 Review Committee (Onsite Panel) National Science Foundation (NSF) 2017 Review Committee (Mail Reviewer) National Institutes of Health (NIH)

Editorial Activities

Year(s) Journal Name Ad Hoc Reviewer (Note: all reviewing activity below occurred from 2012 – Present while at UTSW) ACS Applied Materials & Interfaces ACS Biomaterials Science & Engineering ACS Books ACS Macro Letters ACS Nano Acta Biomaterialia Advanced Materials Advanced Science Angewandte Chemie Bioengineering and Translational Medicine Bioconjugate Chemistry 4 Biomacromolecules Biomaterials Biomaterials Science Cancer Research Cellular and Molecular Bioengineering Chemical Communications Chemical Science Chemistry - A European Journal ChemMedChem Clinical Orthopedics and Related Research Colloid and Polymer Science Drug Delivery European Journal of Pharmaceutics and Biopharmaceutics Experimental Biology and Medicine Gastroenterology Integrative Biology Journal of the American Chemical Society Journal of Biomedical Nanotechnology Journal of Controlled Release Journal of Materials Chemistry B Journal of Visualized Experiments Langmuir Macromolecular Bioscience Macromolecular Rapid Communications Macromolecules Microscopy and Microanalysis Molecular Pharmaceutics Molecular Therapy Molecular Therapy – Gene Therapy Molecular Therapy – Nucleic Acids Nano Letters Nano Research Nanoscale Nature Communications Nature Nanotechnology Nature Reviews Materials Nucleic Acids Research Physiological Reviews Polymer Polymer Chemistry Polymer International Proceedings of the National Academy of Sciences, U.S.A. Progress in Polymer Science Science Advances Scientific Reports WIREs

Invited Lectures 5

Year(s) Title Location International 2014 4th Zing Polymer Chemistry Conference Cancun, Mexico 2016 World Biomaterials Congress Montreal, Quebec, Canada University of British Columbia Vancouver, British Columbia, 2017 Department of Biochemistry Canada European Cystic Fibrosis Society (ECFS) 2017 Albufeira, Portugal Basic Science Conference Beijing Symposium 2017: 2017 Suzhou, China Polymeric Biomaterials 2018 Nano Korea Conference Seoul, Korea Pohang University of Science and Technology 2018 (POSTECH) Pohang, Korea Department of Chemistry Korea Advanced Institute for Science and 2018 Technology (KAIST) Daejeon, Korea Department of Chemistry

National Gordon Research Conference 2013 West Dover, VT on Cancer Nanotechnology 2014 Society for Biomaterials Annual Meeting Denver, CO “Polymers in Medicine”, American Chemical 2015 Santa Rosa, CA Society Polymer Division Workshop “Macromolecular Engineering Symposium Honoring the Impact and 65th Birthday of 2015 Pittsburgh, PA Krzysztof Matyjaszewski”, Carnegie Mellon University 2015 American Chemical Society National Meeting Boston, MA 2015 Society for Biomaterials Annual Meeting Charlotte, NC US-Japan Symposium on Drug Delivery 2015 Lahaina, HI Systems Cystic Fibrosis Foundation Conference: 2016 New Technologies Advancing Toward a One Savannah, GA Time Cure “microRNAs: A Gene Silencing Mechanism 2016 with Therapeutic Implications” conference New York, NY hosted by the New York Academy of Sciences Nanomedicines Symposium: Advances in 2016 Materials, Manufacturing & Therapeutic Seattle, WA Applications

6 American Chemical Society National Meeting, invited lecturer in the “Polymeric Materials as 2016 Philadelphia, PA Imaging Agents & Theranostics” special symposium. Gordon Research Conference on 2016 Waterville Valley, NH Drug Carriers in Medicine & Biology 2017 Society for Biomaterials Annual Meeting Minneapolis, MN 2017 Controlled Release Society Annual Meeting Boston, MA Cystic Fibrosis Foundation Research 2017 Stevenson, WA Conference: Pushing the Frontiers Oregon Health Sciences University 2017 Portland, OR College of Pharmacy Iowa State University 2017 Department of Materials Science and Ames, IA Engineering University of Nebraska Medical Center Department of Pharmacy 2017 Omaha, NE 4th Annual Biopharmaceutical Research and Development Symposium (BRDS) US-Japan Symposium on Drug Delivery 2017 Lahaina, HI Systems 2018 Foundations of Nanoscience Conference Snowbird, Utah University of Notre Dame 2018 South Bend, IN Department of Chemistry “Advancing Gene Editing Technologies for the Treatment of Cystic Fibrosis Lung Disease” 2018 Bethesda, MD workshop co-sponsored by the NIH and Cystic Fibrosis Foundation American Society of Gene & Cell Therapy 2018 Chicago, IL (ASGCT) Annual Meeting Cystic Fibrosis Foundation Research 2018 Conference: New Technologies Advancing Jackson, WY Toward a One-Time Cure Controlled Release Society (CRS) Annual 2018 New York, NY Meeting & Exposition CF Gene Editing Workshop: Advancing Gene Editing Technologies and Tools for Cystic 2018 Fibrosis, sponsored by the National Institutes of Bethesda, MD Health (NIH) and the Cystic Fibrosis Foundation (CFF) 2018 NanoDDS Conference Portland, OR Oligonucleotide Therapeutic Society Annual 2018 Seattle, WA Meeting 2018 mRNA Healthcare Conference Boston, MA 7

Regional/Local University of Texas at Dallas 2013 Richardson, TX Department of Bioengineering 2013 UTSW Biochemistry Department Retreat New Braunfels, TX 2013 UTSW New Faculty Research Forum Dallas, TX 2013 Mirna Therapeutics Austin, TX University of Texas at Arlington 2014 Arlington, TX Department of Bioengineering 2014 UTSW MSTP Program Dallas, TX UTSW Hamon Center/Cancer Center 2014 Dallas, TX Experimental Therapeutics Program American Chemical Society Southwest 2014 Fort Worth, TX Regional Meeting CPRIT Innovations in Cancer Prevention and 2015 Austin, TX Research Conference UTSW Hamon Center/Cancer Center 2015 Dallas, TX Experimental Therapeutics Program Trinity University 2015 San Antonio, TX Department of Chemistry Southern Methodist University 2015 Dallas, TX Department of Chemistry 2015 SURF Presentation to Students Dallas, TX 2016 BioNorthTexas Presentation Dallas, TX The Academy of Medicine, Engineering and 2017 Science of Texas (TAMEST) San Antonio, TX Protege - 2017 Annual Conference University of Texas at Austin 2017 Department of Biomedical Engineering Austin, TX Biomaterials Day Baylor University 2018 Waco, TX Department of Chemistry University of Texas at Dallas 2018 Richardson, TX Department of Chemistry

Technological and Other Scientific Innovations

Innovation Patent, if any, pending or awarded /If described in print/on web, provide citation

App. No. (or Patent No.) / Title Inventor(s)

8 Date of Filing (or Date of Issuance) Lipocationic Polymers and Uses Daniel J. Siegwart, Jing 9,517,270 / December 13, 2016 Thereof (UT Southwestern internal Hao, Kejin Zhou, Jason file reference UTSD:2880 US) Miller, Petra Kos, Lian Xue Lipocationic Polymers and Uses Daniel J. Siegwart, Jing PCT/US2015/064380 / December Thereof (UT Southwestern internal Hao, Kejin Zhou, Jason 8, 2015 file reference UTSD:2880 WO) Miller, Petra Kos, Lian Xue Lipocationic Polymers and Uses Daniel J. Siegwart, Jing 15866947.3 / December 8, 2015 Thereof (UT Southwestern internal Hao, Kejin Zhou, Jason file reference UTSD:2880 EP) Miller, Petra Kos, Lian Xue Lipocationic Polymers and Uses Daniel J. Siegwart, Jing 2,970,177 / December 8, 2015 Thereof (UT Southwestern internal Hao, Kejin Zhou, Jason file reference UTSD:2880 CA) Miller, Petra Kos, Lian Xue Lipocationic Polymers and Uses Daniel J. Siegwart, Jing 2015360794 / December 8, 2015 Thereof (UT Southwestern internal Hao, Kejin Zhou, Jason file reference UTSD:2880 AU) Miller, Petra Kos, Lian Xue Lipocationic Polymers and Uses Daniel J. Siegwart, Jing Thereof (UT Southwestern internal 15/261,245 / September 9, 2016 Hao, Kejin Zhou, Jason file reference UTSD:2880 US Miller, Petra Kos, Lian Xue CON) Lipocationic Dendrimers and Uses Daniel J. Siegwart, Kejin 15/265,064 / September 14, 2016 Thereof (UT Southwestern internal Zhou file reference UTSD:2929 US) Lipocationic Dendrimers and Uses PCT/US2016/051648 / September Daniel J. Siegwart, Kejin Thereof (UT Southwestern internal 14, 2016 Zhou file reference UTSD:2929 WO) Compositions for the Delivery of Daniel J. Siegwart, Philip J. tRNA as Nanoparticles and Thomas, Arthur E. 15/597,063 / May 16, 2017 Methods of Use Therewith (UT Johnson, Michael J. Torres, Southwestern internal file reference Jason B. Miller UTSD:3005 US) Compositions for the Delivery of Daniel J. Siegwart, Philip J. tRNA as Nanoparticles and PCT/US2017/032967 / May 16, Thomas, Arthur E. Methods of Use Therewith (UT 2017 Johnson, Michael J. Torres, Southwestern internal file reference Jason B. Miller UTSD:3005 WO) Cationic Sulfonamide Amino Lipids and Amphiphilic Daniel J. Siegwart, Jason B. 15/597,049 / May 16, 2017 Zwitterionic Amino Lipids (UT Miller Southwestern internal file reference UTSD:3035 US) Cationic Sulfonamide Amino Lipids and Amphiphilic PCT/US2017/032950 / May 16, Daniel J. Siegwart, Jason B. Zwitterionic Amino Lipids (UT 2017 Miller Southwestern internal file reference UTSD:3035 WO)

9

Service to the Community

Organization or Year(s) Role institution May include a brief, one-sentence description of each role if needed (optional)

Gave a research lecture to Biomedical Engineering 2013 UT Dallas students Science Teacher Access to Performed chemistry demonstrations to high school 2014 Resources at Southwestern students (STARS) program UTSW Postdoctoral 10th Annual Postdoctoral Poster Session and Symposium Association, Graduate 2014 presentation entitled “How to Successfully Land a School of Biomedical Faculty Position” Sciences, Postdoctoral Affairs Office Gave a presentation and met with students at the Biomedical and Molecular Imaging Seminar, October 1, 2016 UT Dallas 2016, University of Texas at Dallas. Outreach event for the UTSW Biomedical Engineering graduate program. UTSW Office for Panel presenter, Intellectual Property Day Panel, 2017 Technology Development “Innovation – Improving Lives” (OTD)

Bibliography

Peer-Reviewed Publications

Original Research Articles

Prior to UT Southwestern

1. Sarbu, T; Lin, K; Ell, J; Siegwart, DJ; Spanswick, J; Matyjaszewski, K.* “Polystyrene with designed molecular weight distribution by atom transfer radical coupling.” Macromolecules, 37, 3120-3127 (2004).

2. Sarbu, T; Lin, K; Spanswick, J; Gil, RR; Siegwart, DJ; Matyjaszewski, K.* “Synthesis of hydroxy-telechelic poly(methyl acrylate) and polystyrene by atom transfer radical coupling.” Macromolecules, 37, 9694-9700 (2004). 3. Gao, H; Siegwart, DJ; Jahed, N; Sarbu, T; Matyjaszewski, K.* “Characterization of -,- dihydroxypolystyrene by gradient polymer elution chromatography and two-dimensional liquid chromatography.” Designed Monomers and Polymers, 8, 533-546 (2005).

4. Huang, J; Jia, S; Siegwart, DJ; Kowalewski, T; Matyjaszewski, K.* “Synthesis and characterization of styrene/butyl acrylate linear and star block copolymers via atom transfer 10 radical polymerization.” Macromolecular Chemistry and Physics, 207, 801-811 (2006).

5. Siegwart, DJ; Wu, W; Mandalaywala, M; Tamir, M; Sarbu, T; Silverstein, MS; Kowalewski, T; Hollinger, JO; Matyjaszewski, K.* “Solvent induced morphologies of poly(methyl methacrylate-b-ethylene oxide-b-methyl methacrylate) triblock copolymers synthesized by atom transfer radical polymerization.” Polymer, 48, 7279-7290 (2007).

6. Oh, JK; Siegwart, DJ; Lee, H; Sherwood, G; Peteanu, L; Hollinger, JO; Kataoka, K; Matyjaszewski, K.* “Biodegradable nanogels prepared by atom transfer radical polymerization as potential drug delivery carriers: Synthesis, biodegradation, in vitro release, and bioconjugation.” Journal of the American Chemical Society, 129, 5939-5945 (2007).

7. Oh, JK; Siegwart, DJ; Matyjaszewski, K.* “Synthesis and biodegradation of nanogels as delivery carriers for carbohydrate drugs.” Biomacromolecules, 8, 3326-3331 (2007).

8. Siegwart, DJ; Bencherif, SA; Srinivasen, A; Hollinger, JO; Matyjaszewski, K.* “Synthesis, characterization, and in vitro cell culture viability of degradable poly(N-isopropylacrylamide- co-5,6-benzo-2-methylene-1,3-dioxepane)-based polymers and crosslinked gels.” Journal of Biomedical Materials Research, Part A, 87, 345-358 (2008).

9. Siegwart, DJ; Oh, JK; Bencherif, SA; Bohaty, A.; Hollinger, JO; Matyjaszewski, K.* “Biotin-, pyrene- and GRGDS-functionalized ATRP polymers and nanogels via end group modification.” Macromolecular Chemistry and Physics, 209, 2179-2193 (2008).

10. Siegwart, DJ; Srinivasan, A; Bencherif, SA; Karunanidhi, A; Oh, JK; Vaidya, S; Jin, R; Hollinger, JO; Matyjaszewski, K.* “Cellular uptake of functional nanogels prepared by inverse miniemulsion ATRP with encapsulated proteins, carbohydrates, and gold nanoparticles.” Biomacromolecules, 10, 2300-2309 (2009).

11. Bencherif, SA; Gao, H; Srinivasan, A.; Siegwart DJ; Hollinger, JO; Washburn, NR; Matyjaszewski, K.* “Cell-adhesive star polymers prepared by ATRP.” Biomacromolecules, 10, 1795-1803 (2009).

12. Bencherif, SA; Siegwart, DJ; Srinivasan, A; Horkay, F; Hollinger, JO; Washburn, NR; Matyjaszewski, K.* “Nanostructured hybrid hydrogels prepared by a combination of atom transfer radical polymerization and free radical polymerization.” Biomaterials, 30, 5270- 5278 (2009). 13. Cheng, H; Kastrup, CJ; Ramanathan, R; Siegwart, DJ; Ma, M; Bogatyrev, SR; Xu, Q; Whitehead, KA; Langer, R; Anderson, DG.* “Nanoparticulate cellular patches for cell- mediated tumoritropic delivery.” ACS Nano, 4, 625-631 (2010).

14. Cho, HY; Gao, H; Srinivasan, A; Hong, J; Bencherif, SA; Siegwart, DJ; Paik, H; Hollinger, JO; Matyjaszewski, K.* “Rapid cellular internalization of multifunctional star polymers prepared by atom transfer radical polymerization.” Biomacromolecules, 11, 2199-2203 (2010).

15. Pritchard, CD; O’Shea, TM; Siegwart, DJ; Calo, E; Anderson, DG; Reynolds, FM; Woodard, EJ; Langer, R.* “An injectable thiol-acrylate poly(ethylene glycol) hydrogel for sustained release of methylprednisolone sodium succinate.” Biomaterials, 32, 587-597 (2011). 16. Cheng, H; Hill, PS; Siegwart, DJ; Vacanti, N; Lytton-Jean, AKR; Cho, S-W; Ye, A; Langer, R; Anderson, DG.* “A novel family of biodegradable poly(ester amide) elastomers.” Advanced Materials, 23, H95-H100 (2011).

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17. Ma, M; Liu, WF; Hill, PS; Bratlie, KM; Siegwart, DJ; Chin, J; Park, M; Guerreiro, J; Anderson, DG.* “Development of cationic polymer coatings to regulate foreign-body responses.” Advanced Materials, 23, H189-H194 (2011).

18. Siegwart, DJ; Whitehead, KA; Nuhn, L; Sahay, G; Cheng, H; Jiang, S; Ma, M; Lytton-Jean, AKR; Vegas, A; Fenton, P; Love, KT; Levins, CG; Cortez, C; Lee, H; Collins, SP; Li, YF; Jang, J; Querbes, W; Zurenko, C; Novobrantseva, T; Langer, R; Anderson, DG.* “Combinatorial synthesis of chemically diverse core-shell nanoparticles for intracellular delivery.” Proceedings of the National Academy of Sciences, U.S.A., 108, 12996-13001 (2011).

19. Siegwart, DJ; Leiendecker, M; Langer, R; Anderson, DG.* “Automated ARGET ATRP accelerates catalyst optimization for the synthesis of thiol-functionalized polymers.” Macromolecules, 45, 1254-1261 (2012).

20. Averick, SE; Paredes, E; Irastorza, A; Shrivats, AR; Srinivasan, A; Siegwart, DJ; Magenau, AJ; Cho, HY; Hsu. E; Averick, AA; Kim, J; Liu, S; Hollinger, JO; Das, SR; Matyjaszewski, K.* “Preparation of cationic nanogels for nucleic acid delivery.” Biomacromolecules, 13, 3445-3499 (2012).

21. Eltoukhy, AA; Siegwart, DJ; Alabi, CA; Rajan, JS; Langer, R; Anderson, DG.* “Effect of molecular weight of amine end-modified poly(ß-amino ester)s on gene delivery efficiency and toxicity." Biomaterials, 33, 3594-3603 (2012).

22. Dong, Y; Love, KT; Dorkin, JR; Sirirungruang, S; Zhang, Y; Chen, D; Bogorad, RL; Yin, H; Chen, Y; Vegas, AJ; Alabi, CA; Sahay, G; Olejnik, KT; Wang, W; Schroeder, A; Lytton-Jean, AKR; Siegwart, DJ; Akinc, A; Barnes, C; Barros, SA; Carioto, M; Fitzgerald, K; Hettinger, J; Kumar, V; Novobrantseva, TI; Qin, J; Querbes, W; Koteliansky, V; Langer, R; Anderson, DG.* “Lipopeptide nanoparticles for potent and selective siRNA delivery in rodents and nonhuman primates.” Proceedings of the National Academy of Sciences U.S.A., 111, 3955- 3960 (2014).

Independent research at UT Southwestern

23. Yan, Y; Siegwart, DJ.* “Scalable synthesis and derivation of functional polyesters bearing ene and epoxide side chains.” Polymer Chemistry, 5, 1362-1371 (2014). 24. Rainbolt, EA; Miller, JB; Washington, KE; Senevirathne, SA; Biewer, MC; Siegwart, DJ*; Stefan, MC.* “Fine-tuning thermoresponsive functional poly(ε-caprolactone)s to enhance micelle stability and drug loading.” Journal of Materials Chemistry B, 3, 1779-1787 (2015).

25. Yan, Y; Xue, L; Miller, JB; Zhou, K; Kos, P; Elkassih, S; Liu, L; Nagai, A; Xiong, H; Siegwart, DJ.* “One-pot synthesis of functional poly(amino ester sulfide)s and utility in delivering pDNA and siRNA.” Polymer, 72, 271-280 (2015).

26. Nagai, A*; Miller, JB; Du, J; Kos, P; Stefan, MC; Siegwart, DJ.* “Biocompatible organic charge transfer complex nanoparticles based on a semi-crystalline cellulose template.” Chemical Communications, 51, 11868-11871 (2015).

27. Hao, J; Kos, P; Zhou, K; Miller, JB; Xue, L; Yan, Y; Xiong, H; Elkassih, S; Siegwart, DJ.* “Rapid synthesis of a lipocationic polyester library via ring-opening polymerization of functional valerolactones for efficacious siRNA delivery.” Journal of the American 12 Chemical Society, 137, 9206-9209 (2015).

28. Wu, L; Nguyen, LH; Zhou, K; de Soysa, Y; Li, L; Miller, JB; Tian, J; Locker, J; Zhang, S; Shinoda, G; Seligson, MT; Zeitels, LR; Acharya, A; Wang, SC; Mendell, JT; Nishino, J; Morrison, SJ; Siegwart, DJ; Daley, GC; Shyh-Chang, N; Zhu, H.* “Precise regulation of let- 7 levels balance organ regeneration against tumor suppression.” eLife, 4, e09431 (2015).

29. Nagai, A*; Miller, JB; Kos, P; Elkassih, S; Xiong, H; Siegwart, DJ.* “Tumor imaging based on photon upconversion of Pt(II) porphyrin rhodamine co-modified NIR excitable cellulose enhanced by aggregation.” ACS Biomaterials Science & Engineering, 1, 1206-1210 (2015). 30. Zhou, K; Nguyen, LH; Miller, JB; Yan, Y; Kos, P; Xiong, H; Li, L; Hao, J; Minnig, JT; Zhu, H; Siegwart, DJ.* “Modular degradable dendrimers enable small RNAs to extend survival in an aggressive liver cancer model.” Proceedings of the National Academy of Sciences, U.S.A., 113, 520-525 (2016).

31. Xiong, H; Kos, P; Yan, Y; Zhou, K; Miller, JB; Elkassih, S; Siegwart, DJ.* “Activatable water soluble probes enhance tumor imaging by responding to dysregulated pH and exhibiting high tumor-to-liver fluorescence emission contrast.” Bioconjugate Chemistry, 27, 1737-1744 (2016).

32. Hao, J; Elkassih, S; Siegwart, DJ.* “Progress towards the synthesis of amino polyesters via ring-opening polymerization (ROP) of functional lactones.” Synlett, 27, 2285-2292 (2016).

33. Zhou, K; Kos, P; Yan, Y; Xiong, H; Min, Y; Minnig, JT; Miller, JB; Siegwart, DJ.* “Intercalation-mediated nucleic acid nanoparticles for siRNA delivery.” Chemical Communications, 52, 12155-12158 (2016).

34. Yan, Y; Liu, L; Xiong, H; Miller, JB; Zhou, K; Kos, P; Huffman, KE; Elkassih, S; Norman, JW; Carstens, R; Kim, J; Minna, JD; Siegwart, DJ.* “Functional polyesters enable selective siRNA delivery to lung cancer over matched normal cells.” Proceedings of the National Academy of Sciences, U.S.A., 113, E5702–E5710 (2016).

35. Senevirathne, SA; Washington, KE; Miller, JB; Biewer, MC; Oupicky, D;* Siegwart, DJ;* Stefan, MC.* "HDAC inhibitor conjugated polymeric prodrug micelles for doxorubicin delivery." Journal of Materials Chemistry B, 5, 2106-2114 (2017).

36. Chen, P; Zuo, H; Xiong, H; Kolar, MJ; Chu, Q; Saghatelian, A; Siegwart, DJ; Wan, Y.* “Gpr132 sensing of lactate mediates tumor-macrophage interplay to promote breast cancer metastasis.” Proceedings of the National Academy of Sciences, U.S.A., 114, 580-585 (2017).

37. Yan, Y; Zhou, K; Xiong, H; Miller, JB; Motea, EA; Boothman, DA; Liu, L;* Siegwart, DJ.* “Aerosol delivery of stabilized polyester-siRNA nanoparticles to silence gene expression in orthotopic lung tumors.” Biomaterials, 118, 84-93 (2017).

38. Miller, JB; Zhang, S; Kos, P; Xiong, H; Zhou, K; Perelman, SS; Zhu, H; Siegwart, DJ.* “Non-viral CRISPR/Cas gene editing in vitro and in vivo enabled by synthetic nanoparticle co-delivery of Cas9 mRNA and sgRNA.” Angewandte Chemie International Edition, 56, 1059-1063 (2017). 39. Xiong, H; Zuo, H; Yan, Y; Occhialini, G; Zhou, K; Wan, Y; Siegwart, DJ.* “High-contrast fluorescence detection of metastatic breast cancer including bone and liver micrometastases via size-controlled pH-activatable water-soluble probes.” Advanced Materials, 29, 1700131 (2017). 13

40. Yan, Y; Xiong, H; Zhang, X; Cheng, Q; Siegwart, DJ.* “Systemic mRNA delivery to the lungs by functional polyester-based carriers.” Biomacromolecules, 18, 4307-4315 (2017).

41. Miller, JB; Kos, P; Tieu, V; Siegwart, DJ.* “Development of cationic quaternary ammonium sulfonamide amino lipids (CSALs) for nucleic acid delivery.” ACS Applied Materials & Interfaces, 10, 2302-2311 (2018).

42. Hagemeijer, MC; Siegwart, DJ; Strug, LJ; Cebotaru, L; Torres, MJ; Sofoluwe, A; Beekman, JM.* “Translational research to enable personalized treatment of cystic fibrosis.” Journal of Cystic Fibrosis, 17, S46–S51 (2018).

43. Zhang, S; Nguyen, LH; Zhou, K; Tu, H; Sehgal, A; Nassour, I; Li, L; Gopal, P; Goodman, J; Singal, AG; Yopp, A; Zhang, Y; Siegwart, DJ; Zhu, H.* “Knockdown of anillin actin binding protein blocks cytokinesis in hepatocytes and reduces liver tumor development in mice without affecting regeneration.” Gastroenterology, 154, 1421-1434 (2018).

44. Zhang, S; Zhou, K; Luo, X; Li, L; Tu, HC; Sehgal, A; Nguyen, LH; Zhang, Yu; Gopal, P; Tarlow, B; Siegwart, DJ; Zhu, H. “The polyploid state plays a tumor suppressive role in the liver.” Developmental Cell, 44, 447-459 (2018).

45. Xiong, H; Zhou, K; Yan, Y; Miller, JB; Siegwart, DJ.* “Tumor-activated water-soluble photosensitizers for near-infrared photodynamic cancer therapy.” ACS Applied Materials & Interfaces, 10, 16335-16343 (2018).

46. Wang, Y; Su, l; Morin, MD; Jones, BT; Mifune, Y; Shi, H; Wang, K-W; Zhan, X; Liu, A; Wang, J; Li, X; Tang, M; Ludwig, S; Hildebrand, S; Zhou, K; Siegwart, DJ; Moresco, EMY; Zhang, H; Boger, DL; Bruce Beutler, B.* “Adjuvant effect of the novel TLR1/TLR2 agonist Diprovocim synergizes with anti–PD-L1 to eliminate melanoma in mice.” Proceedings of the National Academy of Sciences, U.S.A., 2018, early view.

Reviews, Chapters, Monographs and Editorials

47. Oh, JK; Drumright, R.; Siegwart, DJ; Matyjaszewski, K.* “The development of microgels/nanogels for drug delivery applications.” Progress in Polymer Science, 33, 448- 477 (2008). 48. Siegwart, DJ; Oh, JK; Matyjaszewski, K.* “ATRP in the design of functional materials for biomedical applications.” Progress in Polymer Science, 37, 18-37 (2012). 49. Siegwart, DJ.* “Polymers for drug delivery.” McGraw-Hill 2015 Yearbook of Science & Technology. New York: McGraw-Hill, (book chapter), ISBN 0071835768 / 9780071835763 (2015). 50. Nagai, A;* Siegwart, DJ. “Theory study and present CT trend of organic charge transfer complexes.” Conjugated Objects: Developments, Synthesis, and Applications. Singapore: Pan Stanford Publishing, 381-412 (book chapter) (2017). 51. Miller, JB; Siegwart, DJ.* “Design of synthetic materials for intracellular delivery of RNAs: From siRNA-mediated gene silencing to CRISPR/Cas gene editing.” Nano Research, 11, 5310–5337 (2018).

Highlights in Video and Print

14 1. My lab’s PNAS paper on microRNA delivery using dendrimer-based nanoparticles to treat liver cancer: • Highlighted in a UT Southwestern press release entitled “Scientists synthesize nanoparticles that can deliver tumor suppressors to damaged livers.” (January 25, 2016) http://www.utsouthwestern.edu/newsroom/articles/year-2016/dendrimers- siegwart.html • Highlighted by at least 13 news outlets in separate articles 2. My lab’s PNAS paper on siRNA delivery using functional polyester carriers to treat lung cancer: • Highlighted in a UT Southwestern press release entitled “Drug-loaded synthetic nanoparticles can distinguish lung cancer cells from healthy cells.” (September 14, 2016) http://www.utsouthwestern.edu/newsroom/articles/year-2016/nanoparticles- siegwart.html • Highlighted in Carnegie Mellon Today, as a part of an alumni profile story entitled “Cancer Breakthrough” (December 6, 2016) http://cmtoday.cmu.edu/science_technology/cancer-breakthrough/ • Highlighted by at least 12 news outlets in separate articles 3. My lab’s Angew. Chem. Int. Ed. paper on CRISPR/Cas gene editing: • Highlighted in Chemical & Engineering News (C&EN)’s February 2017 cover article entitled “CRISPR’s breakthrough problem: If the CRISPR gene editing system is to live up to its disease-curing potential, researchers must devise a plan to deliver it into the body” https://cen.acs.org/articles/95/i7/CRISPRs-breakthrough-problem.html Relevant text from the magazine: “New and improved lipid nanoparticles are popping up that can deliver both Cas9 mRNA and the guide RNA in the same particle. Although that dual packaging would in theory improve editing efficiency, it also poses some logistical problems. The molecules used in RNAi are only about 20 nucleotides long. Guide RNAs used in CRISPR, on the other hand, are about 100 nucleotides long, and the mRNA encoding Cas9 is an unwieldy beast of 4,500 nucleotides. “So if you take the off-the-shelf lipid nanoparticle formulation and instead encapsulate the CRISPR system, it’s just not very good,” says Daniel J. Siegwart of the University of Texas Southwestern Medical Center. Siegwart, who was previously a postdoctoral researcher in Anderson’s lab, became the first to successfully deliver that kind of dual packaging to mice in December. Lipid nanoparticles require several ingredients for ferrying RNA into cells, including positively charged lipids for binding the negatively charged RNA. Siegwart’s group synthesized zwitterionic amino lipids, ones containing both positive and negative charges, which help bind, stabilize, and release the mRNA as the particles cross into cells (Angew. Chem. Int. Ed. 2016, DOI: 10.1002/anie.201610209).” • This paper was selected by the editors and referees of Angew. Chem. Int. Ed.as a Very Important Paper (VIP), which is a special designation given to less than 5% of all accepted papers. • Highlighted by at least 41 news and Twitter outlets 4. My lab’s overall research was highlighted in the UT Southwestern Annual Review 2016:

15 • Annual Review section on “Breaking New Ground in Research and Treatment” (February 7, 2017) http://www.utsouthwestern.edu/life-at/features/2017/nanoparticles- cancer.html

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