ARP36414 P050) Data Sheet

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SNRNP200 antibody - N-terminal region (ARP36414_P050) Data Sheet Product Number ARP36414_P050 Product Name SNRNP200 antibody - N-terminal region (ARP36414_P050) Size 50ug Gene Symbol SNRNP200 Alias Symbols ASCC3L1; BRR2; FLJ11521; HELIC2; RP33; U5-200KD Nucleotide Accession# NM_014014 Protein Size (# AA) 2136 amino acids Molecular Weight 244kDa Product Format Lyophilized powder NCBI Gene Id 23020 Host Rabbit Clonality Polyclonal Official Gene Full Name Small nuclear ribonucleoprotein 200kDa (U5) This is a rabbit polyclonal antibody against SNRNP200. It was validated on Western Blot by Aviva Systems Biology. At Aviva Systems Biology we manufacture rabbit polyclonal antibodies on a large scale (200-1000 Description products/month) of high throughput manner. Our antibodies are peptide based and protein family oriented. We usually provide antibodies covering each member of a whole protein family of your interest. We also use our best efforts to provide you antibodies recognize various epitopes of a target protein. For availability of antibody needed for your experiment, please inquire (). Peptide Sequence Synthetic peptide located within the following region: GDEDVYGEVREEASDDDMEGDEAVVRCTLSANLVASGELMSSKKKDLHPR Pre-mRNA splicing is catalyzed by the spliceosome, a complex of specialized RNA and protein subunits that removes introns from a transcribed pre-mRNA segment. The spliceosome consists of small nuclear RNA proteins (snRNPs) U1, U2, U4, U5 and U6, together with approximately 80 conserved proteins. U5 snRNP Description of Target contains nine specific proteins. This gene encodes one of the U5 snRNP-specific proteins. This protein belongs to the DEXH-box family of putative RNA helicases. It is a core component of U4/U6-U5 snRNPs and appears to catalyze an ATP-dependent unwinding of U4/U6 RNA duplices. Mutations in this gene cause autosomal- dominant retinitis pigmentosa. Partner Proteins CHMP1B,MEGF10,SMNDC1,YWHAB,YWHAG,APC,CD2BP2,MCC,MEPCE,MYC,PIN1,PRKAB1,RNPS1,RN U11,RNU12-2P,SMNDC1,SNRNP40,SNRPB,SRRM1,TCEA1,USP39,YWHAG,tat Reconstitution and Add 50 ul of distilled water. Final anti-SNRNP200 antibody concentration is 1 mg/ml in PBS buffer with 2% Storage sucrose. For longer periods of storage, store at -20C. Avoid repeat freeze-thaw cycles. Lead Time Domestic: within 24 hours delivery International: 3-5 business days Blocking Peptide For anti-SNRNP200 antibody is Catalog # AAP36414 Swissprot Id O75643 Protein Name U5 small nuclear ribonucleoprotein 200 kDa helicase Protein Accession # NP_054733 Purification Affinity Purified Species Reactivity Human, Bovine, Rat, Pig, Horse, Rabbit, Guinea pig, Mouse, Zebrafish Application WB Predicted Homology Based on Immunogen Pig: 100%; Rat: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Bovine: 100%; Rabbit: 100%; Guinea pig: 100%; Zebrafish: 79% Sequence Human COLO205 WB Suggested Anti-SNRNP200 Antibody Titration: 1.0 ug/ml Positive Control: COLO205 Whole Cell Image 1 __________________________________________________________________________________________________________________________________________________________________ This product is for Research Use Only. Not for diagnostic, human, or veterinary use. Optimal conditions of its use should be determined by end users..

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Supplementary File 2A Revised
Supplementary file 2A. Differentially expressed genes in aldosteronomas compared to all other samples, ranked according to statistical significance. Missing values were not allowed in aldosteronomas, but to a maximum of five in the other samples. Acc UGCluster Name Symbol log Fold Change P - Value Adj. P-Value B R99527 Hs.8162 Hypothetical protein MGC39372 MGC39372 2,17 6,3E-09 5,1E-05 10,2 AA398335 Hs.10414 Kelch domain containing 8A KLHDC8A 2,26 1,2E-08 5,1E-05 9,56 AA441933 Hs.519075 Leiomodin 1 (smooth muscle) LMOD1 2,33 1,3E-08 5,1E-05 9,54 AA630120 Hs.78781 Vascular endothelial growth factor B VEGFB 1,24 1,1E-07 2,9E-04 7,59 R07846 Data not found 3,71 1,2E-07 2,9E-04 7,49 W92795 Hs.434386 Hypothetical protein LOC201229 LOC201229 1,55 2,0E-07 4,0E-04 7,03 AA454564 Hs.323396 Family with sequence similarity 54, member B FAM54B 1,25 3,0E-07 5,2E-04 6,65 AA775249 Hs.513633 G protein-coupled receptor 56 GPR56 -1,63 4,3E-07 6,4E-04 6,33 AA012822 Hs.713814 Oxysterol bining protein OSBP 1,35 5,3E-07 7,1E-04 6,14 R45592 Hs.655271 Regulating synaptic membrane exocytosis 2 RIMS2 2,51 5,9E-07 7,1E-04 6,04 AA282936 Hs.240 M-phase phosphoprotein 1 MPHOSPH -1,40 8,1E-07 8,9E-04 5,74 N34945 Hs.234898 Acetyl-Coenzyme A carboxylase beta ACACB 0,87 9,7E-07 9,8E-04 5,58 R07322 Hs.464137 Acyl-Coenzyme A oxidase 1, palmitoyl ACOX1 0,82 1,3E-06 1,2E-03 5,35 R77144 Hs.488835 Transmembrane protein 120A TMEM120A 1,55 1,7E-06 1,4E-03 5,07 H68542 Hs.420009 Transcribed locus 1,07 1,7E-06 1,4E-03 5,06 AA410184 Hs.696454 PBX/knotted 1 homeobox 2 PKNOX2 1,78 2,0E-06
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Lineage-Specific Programming Target Genes Defines Potential for Th1 Temporal Induction Pattern of STAT4
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Mechanism of Regulation of Spliceosome Activation by Brr2 And
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Analysis of RP2 and RPGR Mutations in Five X-Linked Chinese Families
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Supplementary Figures and Table
SUPPLEMENTARY DATA Supplementary Figure 1. ©2014 American Diabetes Association. Published online at http://diabetes.diabetesjournals.org/lookup/suppl/doi:10.2337/db141 -0066/-/DC1 SUPPLEMENTARY DATA Supplementary Figure 2. ©2014 American Diabetes Association. Published online at http://diabetes.diabetesjournals.org/lookup/suppl/doi:10.2337/db142 -0066/-/DC1 SUPPLEMENTARY DATA -/- Supplementary Table 1. Fold increase of Ser/Thr/Tyr phosphorylation in livers of MKP-3 male mice versus wild type male mice fed on a high fat diet (n=5 for each group). Symbol Name Phosphorylation KO/WT ratio Q Value sites Apoptosis ACIN1 Acin1 protein S64 11.4 0.02 T66 8.3 0.02 API5 Apoptosis inhibitor 5 S461 2.2 0.03 S462 1.8 0.03 AIFM3 Apoptosis-inducing factor 3 S30 7.4 0.03 TP53BP2 Apoptosis-stimulating of p53 protein 2 S479 3.7 0.02 ACIN1 Apoptotic chromatin condensation inducer S64S70 5.7 0.02 1 S208 7.1 0.02 S210 7.0 0.02 S479S482S491 105.7 0.03 S729 2.8 0.02 PEA15 Astrocytic phosphoprotein PEA-15 S116 10.8 0.02 BAG3 BAG family molecular chaperone regulator S179 3.3 0.02 3 S353S357 2.3 0.03 S360 2.3 0.03 S390 8.4 0.02 BNIP2 BCL2/adenovirus E1B 19 kDa-interacting S114 3.9 0.02 protein 2 alpha BNIP3 BCL2/adenovirus E1B 19 kDa protein- S60 19.8 0.03 interacting protein 3 S85T86 14.5 0.02 S88 6.1 0.02 BCL2L13 Bcl-2-like protein 13 S387 4.0 0.02 T389 3.1 0.02 CAAP1 Caspase activity and apoptosis inhibitor S183 2.3 0.03 CARD6 Card6 caspase recruitment domain family, S809 3.6 0.03 member 6 CASP8 Caspase-8 S188 2.2 0.02 DAP Death-associated protein S51 5.4 0.02 DAPK2 Death-associated protein kinase 2 S299 3.8 0.02 S349 3.5 0.02 FAF1 FAS-associated factor 1 S269 17.1 0.04 GAS2 Growth arrest-specific protein 2 T282 5.3 0.02 S283 7.4 0.02 S287 5.3 0.02 S289 7.4 0.02 GCH1 GTP cyclohydrolase 1 S24 3.9 0.02 HTT Huntingtin S398S409S411 9.7 0.02 KRT18 Keratin, type I cytoskeletal 18 T9 2.7 0.02 S31S32S35 2.8 0.02 S43S45 3.1 0.02 PDCD5 MCG128907 S119 10.7 0.02 Y126 4.0 0.02 BNIP3I MCG2480, isoform CRA_b S61S62 12.9 0.03 S63S64 8.1 0.02 ©2014 American Diabetes Association.
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Vaccine-Increased Seq ID Unigene ID Uniprot ID Gene Names
BMJ Publishing Group Limited (BMJ) disclaims all liability and responsibility arising from any reliance Supplemental material placed on this supplemental material which has been supplied by the author(s) J Immunother Cancer Vaccine-Increased Seq ID Unigene ID Uniprot ID Gene Names 1_HSPA1A_3303 Hs.274402 P0DMV8 HSPA1A HSP72 HSPA1 HSX70 100_AKAP17A_8227 Hs.522572 Q02040 AKAP17A CXYorf3 DXYS155E SFRS17A XE7 1000_H2AFY_9555 Hs.420272 O75367 H2AFY MACROH2A1 1001_ITPK1_3705 Hs.308122 Q13572 ITPK1 1002_PTPN11_5781 Hs.506852 Q06124 PTPN11 PTP2C SHPTP2 1003_EIF3J_8669 Hs.404056 O75822 EIF3J EIF3S1 PRO0391 1004_TRIP12_9320 Hs.591633 Q14669 TRIP12 KIAA0045 ULF 1006_YEATS2_55689 Hs.632575 Q9ULM3 YEATS2 KIAA1197 1007_SEL1L3_23231 Hs.479384 Q68CR1 SEL1L3 KIAA0746 1008_IDH1_3417 Hs.593422 O75874 IDH1 PICD 101_HSPH1_10808 Hs.36927 Q92598 HSPH1 HSP105 HSP110 KIAA0201 1010_LDLR_3949 Hs.213289 P01130 LDLR 1011_FAM129B_64855 Hs.522401 Q96TA1 NIBAN2 C9orf88 FAM129B 1012_MAP3K5_4217 Hs.186486 Q99683 MAP3K5 ASK1 MAPKKK5 MEKK5 1013_NEFH_4744 Hs.198760 P12036 NEFH KIAA0845 NFH 1014_RAP1B_5908 Hs.369920 P61224 RAP1B OK/SW-cl.11 1015_MCCC1_56922 Hs.47649 Q96RQ3 MCCC1 MCCA 1017_MT1E_4493 Hs.534330 P04732 MT1E 1022_TXNDC5_81567 Hs.150837 Q8NBS9 TXNDC5 TLP46 UNQ364/PRO700 1023_STRA13_201254 Hs.37616 O14503 BHLHE40 BHLHB2 DEC1 SHARP2 STRA13 1024_NPEPPS_9520 Hs.443837 P55786 NPEPPS PSA 1025_YIPF6_286451 Hs.82719 Q96EC8 YIPF6 1026_CLIP1_6249 Hs.524809 P30622 CLIP1 CYLN1 RSN 1027_SRSF7_6432 Hs.309090 Q16629 SRSF7 SFRS7 103_RPS25_6230 Hs.512676 P62851 RPS25 1031_SOCS7_30837
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Supplementary Data
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Contribution of SNRNP200 Sequence Variations to Retinitis Pigmentosa
Eye (2013) 27, 1204–1213 & 2013 Macmillan Publishers Limited All rights reserved 0950-222X/13 www.nature.com/eye 1 1 1 1 LABORATORY STUDY Contribution X Zhang , TYY Lai , SWY Chiang , POS Tam , DTL Liu1,2, CKM Chan1,3, CP Pang1,2, of SNRNP200 C Zhao4 and LJ Chen1,2 sequence variations to retinitis pigmentosa Abstract mutations in two respective patients affirmed that SNRNP200 contributes to a proportion Purpose Mutations in the SNRNP200 gene of overall RP. have been reported to cause autosomal Eye (2013) 27, 1204–1213; doi:10.1038/eye.2013.137; dominant retinitis pigmentosa (adRP). In this published online 26 July 2013 study, we evaluate the mutation profile of SNRNP200 in a cohort of southern Chinese 1Department of Keywords: retinitis pigmentosa; SNRNP200; RP patients. Ophthalmology and Visual mutation Sciences, The Chinese Methods Twenty adRP patients from University of Hong Kong, 11 families and 165 index patients with Hong Kong, China non-syndromic RP with mixed inheritance patterns were screened for mutations in the Introduction 2Department of mutation hotspots of SNRNP200. These Ophthalmology and Visual Retinitis pigmentosa (RP; MIM268000; included exons 12–16, 22–32, and 38–45, Sciences, Prince of Wales Mendelian Inheritance in Man; National Center Hospital, The Chinese which covered the two helicase ATP-binding for Biotechnology Information, Bethesda, MD, University of Hong Kong, domains in DEAD-box and two sec-63 USA) refers to a clinically and genetically Hong Kong, China domains. The targeted regions were diverse group of diffuse retinal dystrophies. amplified by polymerase chain reaction and 3Hong Kong Eye Hospital, The prevalence of RP is about 1/4000 to 1/6000 analyzed by direct DNA sequencing, Hong Kong, China in certain populations1–3 with no significant followed by in silico analyses.
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