Lab 22 –Female Reproductive System II A560 – Fall 2015

I. Introduction II. Learning Objectives III. Slides and Micrographs A. Genital Tract Female Reproductive System II 1. Oviduct () 2. a. General structure b. Phases i. Proliferative phase ii. Secretory phase iii. Onset of menstruation c. 3. B. Mammary Gland IV. Summary Lab 22 –Female Reproductive System II A560 – Fall 2015

I. Introduction Learning Objectives II. Learning Objectives III. Slides and Micrographs A. Genital Tract 1. Identify the layers in the wall of the uterine tube, know the functions of 1. Oviduct (Fallopian tube) the component cells, and recognize the different tubal regions. 2. Uterus a. General structure 2. Recognize the layers of the uterine wall, the functional significance of b. Phases their components, and their changes during the menstrual cycle. i. Proliferative phase ii. Secretory phase iii. Onset of menstruation 3. Recognize histological feature of the cervix, ectocervix, and vagina. c. Cervix 3. Vagina 4. Understand the structure of the mammary gland and how it changes B. Mammary Gland during puberty, pregnancy, and lactation. IV. Summary Lab 22 –Female Reproductive System II A560 – Fall 2015 Keywords I. Introduction II. Learning Objectives III. Slides and Micrographs A. Genital Tract basal layer lactiferous duct 1. Oviduct (Fallopian tube) cervix mammary gland 2. Uterus ectocervix myoepithelial cells a. General structure endometrial glands b. Phases perimetrium i. Proliferative phase fibrous support tissue uterus ii. Secretory phase functional layer vagina iii. Onset of menstruation c. Cervix 3. Vagina B. Mammary Gland IV. Summary Lab 22 –Female Reproductive System II A560 – Fall 2015

I. Introduction II. Learning Objectives III. Slides and Micrographs A. Genital Tract 1. Oviduct (Fallopian tube) 2. Uterus a. General structure b. Phases i. Proliferative phase ii. Secretory phase Slide 60: Oviduct, Ampulla iii. Onset of menstruation c. Cervix 3. Vagina B. Mammary Gland IV. Summary

Slide 59: Oviduct, Isthmus Lab 22 –Female Reproductive System II A560 – Fall 2015 Slide 19: Oviduct, Masson Trichrome I. Introduction II. Learning Objectives mucosa III. Slides and Micrographs A. Genital Tract 1. Oviduct (Fallopian tube) 2. Uterus a. General structure b. Phases i. Proliferative phase ii. Secretory phase iii. Onset of menstruation c. Cervix serosa 3. Vagina B. Mammary Gland IV. Summary

muscularis Lab 22 –Female Reproductive System II A560 – Fall 2015 Slide 19: Oviduct, Masson Trichrome I. Introduction II. Learning Objectives mucosa III. Slides and Micrographs simple columnar epithelium and thin lamina propria A. Genital Tract 1. Oviduct (Fallopian tube) 2. Uterus a. General structure b. Phases i. Proliferative phase ii. Secretory phase iii. Onset of menstruation c. Cervix muscularis 3. Vagina B. Mammary Gland IV. Summary

serosa Lab 22 –Female Reproductive System II A560 – Fall 2015 Slide 19: Oviduct, Masson Trichrome

I. Introduction II. Learning Objectives III. Slides and Micrographs A. Genital Tract 1. Oviduct (Fallopian tube) 2. Uterus a. General structure b. Phases i. Proliferative phase ii. Secretory phase ciliated cells iii. Onset of menstruation cilia help sweep c. Cervix fluid toward 3. Vagina uterus B. Mammary Gland lamina IV. Summary propria

secretory peg cells non‐ciliated; secrete nutritive film Lab 22 –Female Reproductive System II A560 – Fall 2015

I. Introduction II. Learning Objectives III. Slides and Micrographs A. Genital Tract 1. Oviduct (Fallopian tube) Examine slides 59 and 60 of the 2. Uterus oviduct to identify the following a. General structure structure: b. Phases  mucosa i. Proliferative phase ii. Secretory phase Slide 60: Oviduct, Ampulla  muscularis iii. Onset of menstruation  serosa c. Cervix 3. Vagina How do the structures compare from B. Mammary Gland one slide to the other? IV. Summary

Slide 59: Oviduct, Isthmus Lab 22 –Female Reproductive System II A560 – Fall 2015 Slide 1: Uterus, Trichrome I. Introduction II. Learning Objectives III. Slides and Micrographs A. Genital Tract myometrium 1. Oviduct (Fallopian tube) endometrium 2. Uterus a. General structure b. Phases i. Proliferative phase perimetrium ii. Secretory phase iii. Onset of menstruation c. Cervix 3. Vagina B. Mammary Gland IV. Summary Lab 22 –Female Reproductive System II A560 – Fall 2015 Slide 1: Uterus, Trichrome

I. Introduction II. Learning Objectives III. Slides and Micrographs A. Genital Tract 1. Oviduct (Fallopian tube) 2. Uterus a. General structure b. Phases i. Proliferative phase ii. Secretory phase iii. Onset of menstruation endometrial c. Cervix (uterine) 3. Vagina glands B. Mammary Gland IV. Summary

myometrium

endometrium mucosa with simple columnar epithelium and thick underlying lamina propria containing glands Lab 22 –Female Reproductive System II A560 – Fall 2015

I. Introduction Slide 1: Uterus, Trichrome II. Learning Objectives III. Slides and Micrographs A. Genital Tract 1. Oviduct (Fallopian tube) 2. Uterus a. General structure b. Phases i. Proliferative phase ii. Secretory phase perimetrium iii. Onset of menstruation primarily serosa c. Cervix 3. Vagina B. Mammary Gland IV. Summary

endometrium myometrium Lab 22 –Female Reproductive System II A560 – Fall 2015 Layers of Endometrium

I. Introduction II. Learning Objectives III. Slides and Micrographs A. Genital Tract 1. Oviduct (Fallopian tube) functional 2. Uterus layer a. General structure b. Phases i. Proliferative phase basal layer ii. Secretory phase iii. Onset of menstruation c. Cervix 3. Vagina B. Mammary Gland IV. Summary

myometrium Lab 22 –Female Reproductive System II A560 – Fall 2015 Menstrual Cycle I. Introduction II. Learning Objectives III. Slides and Micrographs 1) Menstrual Phase A. Genital Tract Slide 81 1. Oviduct (Fallopian tube) 2. Uterus a. General structure b. Phases i. Proliferative phase 2) Proliferative Phase ii. Secretory phase iii. Onset of menstruation Slide 80 c. Cervix Slide 83 3. Vagina B. Mammary Gland IV. Summary 3) Secretory Phase Slide 82 Slide 84 Lab 22 –Female Reproductive System II A560 – Fall 2015

I. Introduction Slide 80: Endometrium, H&E II. Learning Objectives III. Slides and Micrographs A. Genital Tract 1. Oviduct (Fallopian tube) 2. Uterus a. General structure b. Phases i. Proliferative phase ii. Secretory phase iii. Onset of menstruation c. Cervix myometrium 3. Vagina B. Mammary Gland endometrium IV. Summary perimetrium Lab 22 –Female Reproductive System II A560 – Fall 2015 Slide 80: Endometrium, H&E I. Introduction II. Learning Objectives III. Slides and Micrographs A. Genital Tract 1. Oviduct (Fallopian tube) 2. Uterus a. General structure b. Phases i. Proliferative phase ii. Secretory phase iii. Onset of menstruation c. Cervix endometrial 3. Vagina glands myometrium B. Mammary Gland IV. Summary

• few glands in endometrium • lots of space between glands Lab 22 –Female Reproductive System II A560 – Fall 2015

I. Introduction Slide 82: Endometrium, H&E II. Learning Objectives III. Slides and Micrographs A. Genital Tract 1. Oviduct (Fallopian tube) 2. Uterus a. General structure b. Phases i. Proliferative phase ii. Secretory phase iii. Onset of menstruation c. Cervix 3. Vagina B. Mammary Gland IV. Summary

myometrium

endometrium Lab 22 –Female Reproductive System II A560 – Fall 2015 Slide 82: Endometrium, H&E I. Introduction II. Learning Objectives III. Slides and Micrographs A. Genital Tract 1. Oviduct (Fallopian tube) vascular 2. Uterus lacunae a. General structure b. Phases i. Proliferative phase ii. Secretory phase iii. Onset of menstruation c. Cervix endometrial 3. Vagina B. Mammary Gland glands IV. Summary

• more numerous, jagged glands • presence of thin‐ walled, blood‐filled lacunae in stroma Lab 22 –Female Reproductive System II A560 – Fall 2015 Slide 81: Endometrium, H&E I. Introduction II. Learning Objectives III. Slides and Micrographs A. Genital Tract 1. Oviduct (Fallopian tube) 2. Uterus a. General structure b. Phases i. Proliferative phase ii. Secretory phase iii. Onset of menstruation c. Cervix 3. Vagina B. Mammary Gland myometrium IV. Summary endometrium Lab 22 –Female Reproductive System II A560 – Fall 2015 Slide 81: Endometrium, H&E I. Introduction II. Learning Objectives III. Slides and Micrographs A. Genital Tract 1. Oviduct (Fallopian tube) 2. Uterus a. General structure b. Phases blood in stroma i. Proliferative phase ii. Secretory phase iii. Onset of menstruation c. Cervix 3. Vagina B. Mammary Gland IV. Summary • blood in stroma, not in lacunae • thick, well‐developed functional layer Lab 22 –Female Reproductive System II A560 – Fall 2015 Slide 133: Cervix, H&E

I. Introduction II. Learning Objectives III. Slides and Micrographs A. Genital Tract 1. Oviduct (Fallopian tube) 2. Uterus a. General structure b. Phases cervical i. Proliferative phase glands ii. Secretory phase iii. Onset of menstruation c. Cervix 3. Vagina B. Mammary Gland IV. Summary

transition of cut edge epithelium stratified squamous epithelium Lab 22 –Female Reproductive System II A560 – Fall 2015 Slide 133: Cervix, H&E I. Introduction II. Learning Objectives III. Slides and Micrographs endocervical gland A. Genital Tract 1. Oviduct (Fallopian tube) 2. Uterus a. General structure ectocervix b. Phases i. Proliferative phase ii. Secretory phase iii. Onset of menstruation c. Cervix 3. Vagina B. Mammary Gland IV. Summary Lab 22 –Female Reproductive System II A560 – Fall 2015 Slide 108: Mammary Gland, H&E I. Introduction II. Learning Objectives III. Slides and Micrographs A. Genital Tract 1. Oviduct (Fallopian tube) fibrous 2. Uterus interlobular a. General structure support tissue b. Phases secretory acini i. Proliferative phase (lobule) ii. Secretory phase lobule consists of iii. Onset of menstruation multiple acini all c. Cervix draining to same 3. Vagina terminal duct B. Mammary Gland IV. Summary adipose Lab 22 –Female Reproductive System II A560 – Fall 2015 Slide 108: Mammary Gland, H&E

I. Introduction II. Learning Objectives III. Slides and Micrographs A. Genital Tract fibrous 1. Oviduct (Fallopian tube) secretory support tissue 2. Uterus acini a. General structure (lobules) b. Phases i. Proliferative phase ii. Secretory phase lactiferous iii. Onset of menstruation duct c. Cervix 3. Vagina B. Mammary Gland IV. Summary Lab 22 –Female Reproductive System II A560 – Fall 2015 Slide 108: Mammary Gland, H&E I. Introduction II. Learning Objectives III. Slides and Micrographs A. Genital Tract 1. Oviduct (Fallopian tube) myoepithelial 2. Uterus cell inside a. General structure basement b. Phases membrane i. Proliferative phase plasma secretory acini and ii. Secretory phase cells ducts are both iii. Onset of menstruation composed of two c. Cervix layers of cells: the 3. Vagina luminal epithelial cells and the basal B. Mammary Gland myoepithelial cells IV. Summary Lab 22 –Female Reproductive System II Common Confusion: A560 – Fall 2015 Seminal vesicle vs. Ampulla (of Oviduct) I. Introduction II. Learning Objectives Seminal vesicle: secretory gland of male reproductive III. Slides and Micrographs tract, producing majority of seminal fluid volume; consists of single tube coiled upon itself; joins with vas deferens to A. Genital Tract form ejaculatory duct 1. Oviduct (Fallopian tube) 2. Uterus Look for: (1) glandular with the appearance of multiple a. General structure lumens as result of coiling of tube; (2) highly‐folded mucosa, often having fine “honeycomb” appearance; (3) b. Phases non‐ciliated pseudostratified columnar epithelium with i. Proliferative phase lipid droplets in cytoplasm giving “foamy” appearance; (4) ii. Secretory phase lipofuscin pigment is often seen in mucosa iii. Onset of menstruation Seminal Vesicle c. Cervix 3. Vagina Ampulla of oviduct (uterine tube, Fallopian tube,or B. Mammary Gland salpinx): tube leading from ovary to uterus; tube transmits IV. Summary ovum to uterus and is the usual site of fertilization, usually in the ampulla

Look for: (1) single lumen tube w/ mucosa surrounded by muscularis and serosa continuous with broad ; (2) mucosal folds often appear “thicker” than in seminal vesicle due to more lamina propria in the folds; (3) ciliated simple columnar epithelium and non‐ciliated “peg cells” present in mucosa; (4) size of muscularis changes with region of oviduct, generally thin in ampulla and thick in Oviduct isthmus Lab 22 ‐ Characteristics of uterine wall throughout menstrual cycle

Time (Days) of Cycle Influencing Hormones Thickness of Endometrium Endometrial Glands Vasculature

Proliferative phase

Secretory phase

Menstrual phase