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Proteínas De Superfície De Paracoccidioides Brasiliensis

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Proteínas De Superfície De Paracoccidioides Brasiliensis UNIVERSIDADE DE BRASÍLIA FACULDADE DE MEDICINA PROGRAMA DE PÓS-GRADUAÇÃO EM PATOLOGIA MOLECULAR Proteínas de superfície de Paracoccidioides brasiliensis CANDIDATA: NADYA DA SILVA CASTRO ORIENTADORA: DRA. CÉLIA MARIA DE ALMEIDA SOARES TESE APRESENTADA AO PROGRAMA DE PÓS-GRADUAÇÃO EM PATOLOGIA MOLECULAR, DA FACULDADE DE MEDICINA, DA UNIVERSIDADE DE BRASÍLIA COMO REQUISITO PARCIAL À OBTENÇÃO DO TÍTULO DE DOUTOR EM PATOLOGIA MOLECULAR. BRASÍLIA – DF MAIO 2008 TRABALHO REALIZADO NO LABORATÓRIO DE BIOLOGIA MOLECULAR, DEPARTAMENTO DE BIOQUÍMICA E BIOLOGIA MOLECULAR, INSTITUTO DE CIÊNCIAS BIOLÓGICAS, DA UNIVERSIDADE FEDERAL DE GOIÁS. APOIO FINANCEIRO: CAPES/ CNPQ/ FINEP/ FAPEG/ SECTEC-GO. II BANCA EXAMINADORA TITULARES Profa. Dra. Célia Maria de Almeida Soares, Instituto de Ciências Biológicas, Universidade Federal de Goiás. Prof. Dr. Augusto Schrank Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul Prof. Dr. Ivan Torres Nicolau de Campos Instituto de Ciências Biológicas, Universidade Federal de Goiás. Prof. Dr. Bergmann Morais Ribeiro Instituto de Ciências Biológicas, Universidade de Brasília. Prof. Dra. Anamélia Lorenzetti Bocca Instituto de Ciências Biológicas, Universidade de Brasília. SUPLENTE Prof. Dr. Fernando Araripe Gonçalves Torres Instituto de Ciências Biológicas, Universidade de Brasília. III ´- Os homens do seu planeta ² disse o pequeno Príncipe ² cultivam cinco mil rosas num jardim... e não encontram o que procuram... - É verdade ² respondi. - E, no entanto, o que eles procuram poderia ser encontrado numa só rosa, ou num pouco de água... - É verdade. E o principezinho acrescentou: Mas os olhos são cegos. eSUHFLVRYHUFRPRFRUDomRµ ³O pequeno príncipe´ de Antonie de Saint-Exupéry IV Dedico esta tese aos meus queridos pais, Nadson e Genialda, que foram e são exemplos de dedicação e de perseverança e cujos incentivos, apoio e amor contribuíram em muito para a realização deste trabalho. V AGRADECIMENTOS À prof. Célia Soares, pela valiosa e dedicada orientação durante o decorrer desta tese, sobretudo nesta reta final. Com palavras talvez eu não consiga exprimir em totalidade minha gratidão, mas deixo aqui, os meus sinceros agradecimentos a você, quem admiro e respeito pelo exemplo de competência e entusiasmo. Às profs. Maristela, Rosália, Fabrícia e Úrsula pela disposição, sugestões, por todo auxílio prestado durante a execução deste trabalho e acima de tudo pela valiosa amizade e conselhos. Aos profs. Sônia, Marilene e Augusto por me acolherem tão bem em seus laboratórios e por contribuírem para o meu aperfeiçoamento profissional. Aos pesquisadores Luciano Feitosa,Marina Vai e Ivan Orlandi pela prontidão e essencial colaboração científica. Aos meus queridíssimos amigos, Kelly, Pati Zambuzzi, Regilda, Sarah, Cristina e Pati Lima, pelo carinho, apoio e pela estimada amizade. Obrigada por tornarem os dias mais agradáveis. Ao Luiz, Wesley, Rogério Fiúza e Pati Kott pelRDX[tOLR³TXHEUD-JDOKRV´macetes e troca de experiências e idéias. A Sabrina, Ronney, Rogério Troian e Rodrigo pela convivência prazerosa durante estes anos e pelos momentos cômicos. Clayton, Alexandre, Juliana e Mônica Santiago pela constante ajuda e dedicação as atividades gerais do laboratório, o que contribui em muito para a realização do trabalho de todos. À Lilian, Zilminha, Ju Camargos, Bruno, Moniquinha, Lidiane, Aline, Sheyla, Glaciane, Kleber, Milce, Bernadete, Karinne, Fernanda, Cristielly, Kesser, Tércio e a tantos outros que já não mais desfrutamos de suas agradáveis companhias no laboratório, mas que deixaram lembranças de muitos bons momentos e de grandes ensinamentos. VI Também a Nathalie, Elisa e Mirelle pelo carinho, fácil sorriso e ajuda em muitos momentos. E aos mais recentes amigos Netto, Mariana, Raquel, Amanda, Ana Flávia, Dayane e Leandro pelo agradável convívio e amizade. Não posso esquecer também dos amigos de Porto Alegre: Julia, Mel, Cris, Irina, Charley, Márcia e a outros que eu possa ter esquecido de citar os nomes, agradeço pelos ótimos momentos de convivência, receptividade e pelo apoio durante a execução dos experimentos. De modo especial, agradeço à Lívia, uma pessoa fantástica que contribuiu muito para o êxito dos meus experimentos. À minha família pelos incentivos e apoio em todos os momentos, e por fazer a vida valer a pena. E ao que merece a minha maior gratidão, Deus, meu alicerce, minha luz... meu rochedo nos momentos alegres e difíceis. Obrigada por estar sempre comigo e por me provar que com fé e coragem a gente chega a muitos lugares e alcança grandes sonhos! A todos que contribuíram direta ou indiretamente para a realização deste trabalho, os meus sinceros agradecimentos! VII PRODUÇÃO CIENTÍFICA DURANTE O DOUTORAMENTO Artigos completos publicados em periódicos 1. Castro NS, Barbosa MS, Maia ZA, Báo SN, Felipe MSS, Santana JM, Mendes- Giannini MJS, Pereira M, Soares CMA. Characterization of Paracoccidioides brasiliensis PbDfg5p, a cell-wall protein implicated in filamentous growth. Yeast 24:141-54, 2008. 2. Santos MO, Castro NS, Pereira M, Soares CMA. Genes involved in translation of Mycoplasma hyopneumoniae and Mycoplasma synoviae. Genetics and Molecular Biology 30:230-5, 2007. 3. Oliveira JC, Castro NS, Felipe MSS, Pereira M, Soares CMA. Comparative analysis of the cDNA encoding a ClpA homologue of Paracoccidioides brasiliensis. Mycological Research 109:707-16, 2005. 4. Castro NS, Maia ZA, Pereira M, Soares CMA. Screening for glycosylphosphatidylinositol (GPI)-anchored proteins in Paracoccidioides brasiliensis. Genetics and Molecular Research 4:326-45, 2005. 5. Felipe MSS, Andrade RV, Arraes FBM, Nicola AM, Maranhão AQ, Torres FA, Silva-Pereira I, Poças-Fonseca MJ, Campos EG, Moraes LMP, Andrade PA, Tavares AHFP, Silva SS, Kyaw CM, Souza DP, PbGenome Network*, Pereira M, Jesuíno RSA, Andrade EV, Parente JA, Oliveira GS, Barbosa MS, Martins NF, Fachin AL, Cardoso RS, Passos GAS, Almeida NF, Walter MEMT, Soares CMA, Carvalho MJA, Brigido MM. Transcriptional profile of the human pathogenic fungus Paracoccidioides brasiliensis in mycelium and yeast cells. The Journal of Biological Chemistry 280:24706-14, 2005. * PbGenome Network: Alda M.T. Ferreira, Alessandra Dantas, Alessandra J. Baptista, Alexandre M. Bailão, Ana L. Bonato, André C. Amaral, Bruno S. Daher, Camila M. Silva, Christiane S. Costa, Clayton L. Borges, Cléber O. Soares, Cristina M. Junta, Daniel A.S. Anjos, Edans F.O. Sandes, VIII Eduardo A. Donadi, Elza T. Sakamoto-Hojo, Flábio R. Araújo, Flávia C. Albuquerque, Gina C. Oliveira, João R.M. Almeida, Juliana C. Oliveira, Kláudia G. Jorge, Larissa Fernandes, Lorena S. Derengowski, Luís A.M. Bataus, Marcus A.M. Araújo, Marcus K. Inoue, Marlene T. De-Souza, Mauro F. Almeida, Nádia S. Parachin, Nadya S. Castro, Odair P. Martins, Patrícia L.N. Costa, Paula Sandrin-Garcia, Renata B.A. Soares, Stephano S. Mello, Viviane C.B. Reis. Artigo submetido para publicação 1. Castro NS, Castro KP, Orlandi I, Feitosa LS, Rosa e Silva LK, Vainstein MH, Báo SN, Vai M, Soares CMA. The beta-1,3-glucanosyltransferase 3 of Paracoccidioides brasiliensis is involved in cell wall biosynthesis and morphogenesis. FEMS Yeast Research (Submetido), 2008. Manuscrito em preparação 1. Castro KP, Castro NS, Feitosa LS, Rosa e Silva LK, Vainstein MH, Báo SN, Soares CMA. The proline-rich cell-wall protein of Paracoccidioides brasiliensis. Trabalhos apresentados em eventos 1. Castro NS, Castro KP, Maia ZA, Felipe MSS, Pereira M, Niño-Vega G, Soares CMA. A family of beta-1,3-glucanosyltransferases of Paracoccidioides brasiliensis: Identification, comparative analysis and heterologous expression. In: IX International Meeting on Paracoccidioidomycosis. Águas de Lindóia. Journal of the São Paulo Institute of Tropical Medicine. São Paulo: v.47. p.50, 2005. 2. Castro KP, Castro NS, Maia ZA, Felipe MSS, Pereira M, Soares CMA. A Proline- rich antigen (PRA/Ag2) homologue of Paracoccidioides brasiliensis In: IX International Meeting on Paracoccidioidomycosis. Águas de Lindóia. Journal of the São Paulo Institute of Tropical Medicine. São Paulo: v.47. p.51, 2005. IX 3. Maia ZA, Castro NS, Castro KP, Felipe MSS, Pereira M, Soares CMA. Characterization and heterologous expression of a recombinant DFG5-like protein of Paracoccidioides brasiliensis. In: IX International Meeting on Paracoccidioidomycosis. Águas de Lindóia. Journal of the São Paulo Institute of Tropical Medicine. São Paulo: v.47. p.50, 2005. 4. Parente JA, Costa M, Castro NS, Felipe MSS, Pereira M, Deepe Jr GS, Soares CMA. Characterization of a serine proteinase from Paracoccidioides brasiliensis accumulated during the infective process. In: IX International Meeting on Paracoccidioidomycosis. Águas de Lindóia. Journal of the São Paulo Institute of Tropical Medicine. São Paulo: v.47. p.54, 2005. 5. Tacco BACA, Costa M, Parente JA, Felipe MSS, Castro NS, Pereira M, Soares CMA. Characterization of an aspartyl proteinase of Paracoccidioides brasiliensis. In: IX International Meeting on Paracoccidioidomycosis. Águas de Lindóia. Journal of the São Paulo Institute of Tropical Medicine. São Paulo: v.47. p.51, 2005. 6. Rezende TCV, Castro NS, Moreira SFI, Santos MO, Felipe MSS, Oliveira EHD, Pereira M, Soares CMA. Identification of new antigens of Paracoccidioides brasiliensis by using the IVIAT technology. In: IX International Meeting on Paracoccidioidomycosis. Águas de Lindóia. Journal of the São Paulo Institute of Tropical Medicine. São Paulo: v.47. p.52, 2005. 7. Chagas RF, Bailão AM, Pereira M, Felipe MSS, Castro NS, Soares CMA. The catalase A of Paracoccidioides brasiliensis: proteomic
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