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(12) United States Patent (10) Patent No.: US 9,161,992 B2 Jefferies Et Al

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(12) United States Patent (10) Patent No.: US 9,161,992 B2 Jefferies Et Al US009 161992B2 (12) United States Patent (10) Patent No.: US 9,161,992 B2 Jefferies et al. (45) Date of Patent: Oct. 20, 2015 (54) P97 FRAGMENTS WITH TRANSFER 4,683.202 A 7, 1987 Mullis ACTIVITY 4,704,362 A 11/1987 Itakura et al. 4,766,075 A 8, 1988 Goeddeletal. (71) Applicant: biosis Technologies, Inc., Richmond 4,800,1594,784.950 A 11/19881/1989 MullisHagen et al. (CA) 4,801,542 A 1/1989 Murray et al. 4.866,042 A 9, 1989 Neuwelt (72) Inventors: Wilfred Jefferies, South Surrey (CA); 4,935,349 A 6/1990 McKnight et al. Mei Mei Tian, Coquitlam (CA): 4.946,778 A 8, 1990 Ladner et al. Timothy Vitalis, Vancouver (CA) 5,091,513 A 2f1992 Huston et al. 5,132,405 A 7, 1992 Huston et al. (73) Assignee: biOasis Technologies, Inc., British 5, 186,941 A 2f1993 Callahan et al. Columbia (CA) 5,672,683 A 9, 1997 Friden et al. 5,677,171 A 10, 1997 Hudziak et al. c - r 5,720,937 A 2f1998 Hudziak et al. (*) Notice: Subject to any disclaimer, the term of this 5,720,954. A 2f1998 Hudziak et al. patent is extended or adjusted under 35 5,725,856 A 3, 1998 Hudziak et al. U.S.C. 154(b) by 0 days. 5,770,195 A 6/1998 Hudziak et al. 5,772,997 A 6/1998 Hudziak et al. (21) Appl. No.: 14/226,506 5,844,093 A 12/1998 Kettleborough et al. 5,962,012 A 10, 1999 Lin et al. (22) Filed: Mar. 26, 2014 5,981, 194 A 11/1999 Jefferies et al. 6,015,557 A 1/2000 Tobinicket al. (65) Prior Publication Data 6,165,464 A 12/2000 Hudziak et al. 6,177,077 B1 1/2001 Tobinick US 2015/0056218 A1 Feb. 26, 2015 6.261,595 B1 7/2001 Stanley et al. 6,387,371 B1 5, 2002 Hudziak et al. 6,399,063 B1 6/2002 Hudziak et al. Related U.S. Application Data 6,419,934 B1 7/2002 Tobinick (63) Continuation of application No. 13/566,260, filed on 6,419,944 B2 7/2002 Tobinick Aug 3, 2012, now Pat. No. 8,722,019 6,455,494 B1 9, 2002 Jefferies et al. • - 9 s • v Y Y’s a 4-3 W J - 6,537,549 B2 3/2003 Tobinick (60) Provisional application No. 61/515,792, filed on Aug. 6,596,541 B2 7/2003 Murphy et al. 5, 2011. 6,765,087 B1 7/2004 Casterman et al. 6,838,254 B1 1/2005 Hamers et al. (51) Int. Cl. 6,982,089 B2 1/2006 Tobinick A6 IK 47/48 (2006.01) (Continued) A6 IK38/17 (2006.01) C07K 4/705 (2006.01) FOREIGN PATENT DOCUMENTS C07K I4/79 (2006.01) C07K 6/8 (2006.01) GB 2188637 10, 1987 A6 IK39/44 (2006.01) WO WO 89,04663 6, 1989 A 6LX39/395 (2006.01) (Continued) A6 IK38/40 (2006.01) A61 K38/00 (2006.01) OTHER PUBLICATIONS (52) U.S. Cl. CPC ....... A61 K47748276 (2013.01); A61K 38/1793 International Search Report for International Application No. PCT/ (2013.01); A61 K38/40 (2013.01); A61 K US2003/00894, mailed Sep. 10, 2003, 3 pages. 39/3955 (2013.01); A61K.39/39533 (2013.01); (Continued) A61K 39/44 (2013.01); C07K 14/70575 (2013.01); C07K 16/18 (2013.01); A61 K38/00 (2013.01); C07K 14/79 (2013.01); C07K 2319/00 (2013.01); C07K 2319/55 (2013.01); Primary Examiner — Amber D Steele C07K 2319/60 (2013.01); C07K 2319/70 Assistant Examiner — Schuyler Milton (2013.01); Y10S 977/773 (2013.01); Y10S (74) Attorney, Agent, or Firm — Cooley LLP 977/92 (2013.01); Y10S 977/927 (2013.01) (58) Field of Classification Search None (57) ABSTRACT See application file for complete search history. The present invention is related to fragments of human mel (56) References Cited anotransferrin (p97). In particular, this invention relates to treatment of diseases through the introduction of the melan U.S. PATENT DOCUMENTS otransferrin fragment conjugated to a therapeutic or diagnos 4,391904. A 7, 1983 Litman et al. tic agent to a Subject. 4,394,448 A 7/1983 Szoka, Jr. et al. 4,399.216 A 8, 1983 Axel et al. 4,683, 195 A 7, 1987 Mullis et al. 16 Claims, 11 Drawing Sheets US 9,161,992 B2 Page 2 (56) References Cited Bickel, U. et al., “In vivo demonstration of subcellular localization of anti-transferrin receptor monoclonal antibody-colloidal gold conju U.S. PATENT DOCUMENTS gate in brain capillary endothelium.” Journal of Histochemistry and Cytochemistry, 42(11): 1493-1497 (1994). 7,132,511 B2 11/2006 Carr et al. 7,138,371 B2 11/2006 DeFrees et al. 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