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(Musa AAA) As Influenced by Agronomic Factors
Institut für Nutzpflanzenwissenschaften und Ressourcenschutz der Rheinischen Friedrich-Wilhelms-Universität Bonn The importance of the antagonistic potential in the management of populations of plant-parasitic nematodes in banana (Musa AAA) as influenced by agronomic factors Inaugural-Dissertation zur Erlangung des Grades Doktor der Agrarwissenschaften (Dr. agr.) der Hohen Landwirtschaftlichen Fakultät der Rheinischen Friedrich- Wilhelms-Universität zu Bonn vorgelegt am 15. Juni 2010 von Anthony Barry Pattison South Johnstone Australia Referent: Prof. Dr. R.A. Sikora Korreferent: Prof. Dr. H. Goldbach Tag der mündlichen Prüfung: 15 September 2011 Erscheinungsjahr: 2011 Dedication: This work is dedicated to the support given to me by family and friends. Especially to my wife Susan, daughters Katie and Emily for their patience while I completed this work. Also, to the friends I have made along the way, who have helped to make the world a little smaller. Summary Dr.agr. Thesis: A Pattison The importance of the antagonistic potential in the management of populations of plant-parasitic nematodes in banana ( Musa AAA) as influenced by agronomic factors Plant-parasitic nematodes are a major obstacle to sustainable banana production around the world. The use of organic amendments was investigated as one method to stimulate organisms that are antagonistic to plant-parasitic nematodes. Nine different amendments; mill mud, mill ash (by-products from processing sugarcane), biosolids, municipal waste (MW) compost, banana residue, grass hay, legume hay, molasses and calcium silicate (CaSi) were applied in a glasshouse experiment. Significant suppression of Radopholus similis occurred in soils amended with legume hay, grass hay, banana residue and mill mud relative to untreated soil, which increased the nematode community structure index, indicating greater potential for predation. -
Influence of Pratylenchus Vulnus and Meloidogyne Hapla on the Growth of Rootstocks of Rose~ G
influence of Pratylenchus vulnus and Meloidogyne hapla on the Growth of Rootstocks of Rose~ G. S. SANTO 2 and BERT LEAR '~ Abstract: Pratylenchus vulnus is involved in a disease of Rosa noisettiana 'Manetti" rose rootstock characterized by darkening of roots, death of feeder toots, and stunting of entire plants. The disease is more severe when plants are grown in silt loam soil than when they are grown in sandy loam soil. The nematodes reproduce best in silt loam soil at 20 C. Meloidogyne hapla did not affect the growth of Manetti. Rosa sp. 'Dr. Huey', Manetti, and R. odorata rose rootstocks were found to be good hosts for P. vulnus whereas R. multiflora was less suitable. M. hapla re- produced well on R. odorata, Dr. Huey, and R. multi[lora, but not on Maneni. Key Words: root- lesion nematode, root-knot nematode, reproduction, soil temperature, soil type. Pratylenchus vulnus Allen and Jensen roses was associated with a reduction in the was first reported from rose roots in Cali- population of M. hapla and Xiphinema fornia in 1953 (14). A 1970 survey of com- arnericanum Cobb as a result of multiple mercial rose greenhouses in northern Cali- applications of 1,2-dibromo-3-chloropro- fornia shows that this nematode is now pane (DBCP) (8). widely distributed (9), and Allen and Jen- Rosa noisettiana Thory 'Manetti' is the sen (l) also report that P. vulnus is widely most popular rootstock used in growing distributed on field-grown roses in southern greenhouse roses for cut flowers. R. odorata California. Sweet and Rosa sp. -
Nematodes and Agriculture in Continental Argentina
Fundam. appl. NemalOl., 1997.20 (6), 521-539 Forum article NEMATODES AND AGRICULTURE IN CONTINENTAL ARGENTINA. AN OVERVIEW Marcelo E. DOUCET and Marîa M.A. DE DOUCET Laboratorio de Nematologia, Centra de Zoologia Aplicada, Fant/tad de Cien.cias Exactas, Fisicas y Naturales, Universidad Nacional de Cordoba, Casilla df Correo 122, 5000 C6rdoba, Argentina. Acceplecl for publication 5 November 1996. Summary - In Argentina, soil nematodes constitute a diverse group of invertebrates. This widely distributed group incJudes more than twO hundred currently valid species, among which the plant-parasitic and entomopathogenic nematodes are the most remarkable. The former includes species that cause damages to certain crops (mainly MeloicU:igyne spp, Nacobbus aberrans, Ditylenchus dipsaci, Tylenchulus semipenetrans, and Xiphinema index), the latter inc1udes various species of the Mermithidae family, and also the genera Steinernema and Helerorhabditis. There are few full-time nematologists in the country, and they work on taxonomy, distribution, host-parasite relationships, control, and different aspects of the biology of the major species. Due tO the importance of these organisms and the scarcity of information existing in Argentina about them, nematology can be considered a promising field for basic and applied research. Résumé - Les nématodes et l'agriculture en Argentine. Un aperçu général - Les nématodes du sol représentent en Argentine un groupe très diversifiè. Ayant une vaste répartition géographique, il comprend actuellement plus de deux cents espèces, celles parasitant les plantes et les insectes étant considèrées comme les plus importantes. Les espèces du genre Me/oi dogyne, ainsi que Nacobbus aberrans, Dùylenchus dipsaci, Tylenchulus semipenetrans et Xiphinema index représentent un réel danger pour certaines cultures. -
"Structure, Function and Evolution of the Nematode Genome"
Structure, Function and Advanced article Evolution of The Article Contents . Introduction Nematode Genome . Main Text Online posting date: 15th February 2013 Christian Ro¨delsperger, Max Planck Institute for Developmental Biology, Tuebingen, Germany Adrian Streit, Max Planck Institute for Developmental Biology, Tuebingen, Germany Ralf J Sommer, Max Planck Institute for Developmental Biology, Tuebingen, Germany In the past few years, an increasing number of draft gen- numerous variations. In some instances, multiple alter- ome sequences of multiple free-living and parasitic native forms for particular developmental stages exist, nematodes have been published. Although nematode most notably dauer juveniles, an alternative third juvenile genomes vary in size within an order of magnitude, com- stage capable of surviving long periods of starvation and other adverse conditions. Some or all stages can be para- pared with mammalian genomes, they are all very small. sitic (Anderson, 2000; Community; Eckert et al., 2005; Nevertheless, nematodes possess only marginally fewer Riddle et al., 1997). The minimal generation times and the genes than mammals do. Nematode genomes are very life expectancies vary greatly among nematodes and range compact and therefore form a highly attractive system for from a few days to several years. comparative studies of genome structure and evolution. Among the nematodes, numerous parasites of plants and Strikingly, approximately one-third of the genes in every animals, including man are of great medical and economic sequenced nematode genome has no recognisable importance (Lee, 2002). From phylogenetic analyses, it can homologues outside their genus. One observes high rates be concluded that parasitic life styles evolved at least seven of gene losses and gains, among them numerous examples times independently within the nematodes (four times with of gene acquisition by horizontal gene transfer. -
Theory Manual Course No. Pl. Path
NAVSARI AGRICULTURAL UNIVERSITY Theory Manual INTRODUCTORY PLANT NEMATOLOGY Course No. Pl. Path 2.2 (V Dean’s) nd 2 Semester B.Sc. (Hons.) Agri. PROF.R.R.PATEL, ASSISTANT PROFESSOR Dr.D.M.PATHAK, ASSOCIATE PROFESSOR Dr.R.R.WAGHUNDE, ASSISTANT PROFESSOR DEPARTMENT OF PLANT PATHOLOGY COLLEGE OF AGRICULTURE NAVSARI AGRICULTURAL UNIVERSITY BHARUCH 392012 1 GENERAL INTRODUCTION What are the nematodes? Nematodes are belongs to animal kingdom, they are triploblastic, unsegmented, bilateral symmetrical, pseudocoelomateandhaving well developed reproductive, nervous, excretoryand digestive system where as the circulatory and respiratory systems are absent but govern by the pseudocoelomic fluid. Plant Nematology: Nematology is a science deals with the study of morphology, taxonomy, classification, biology, symptomatology and management of {plant pathogenic} nematode (PPN). The word nematode is made up of two Greek words, Nema means thread like and eidos means form. The words Nematodes is derived from Greek words ‘Nema+oides’ meaning „Thread + form‟(thread like organism ) therefore, they also called threadworms. They are also known as roundworms because nematode body tubular is shape. The movement (serpentine) of nematodes like eel (marine fish), so also called them eelworm in U.K. and Nema in U.S.A. Roundworms by Zoologist Nematodes are a diverse group of organisms, which are found in many different environments. Approximately 50% of known nematode species are marine, 25% are free-living species found in soil or freshwater, 15% are parasites of animals, and 10% of known nematode species are parasites of plants (see figure at left). The study of nematodes has traditionally been viewed as three separate disciplines: (1) Helminthology dealing with the study of nematodes and other worms parasitic in vertebrates (mainly those of importance to human and veterinary medicine). -
Silencing Parasitism Effectors of the Root Lesion Nematode, Pratylenchus Thornei
Silencing parasitism effectors of the root lesion nematode, Pratylenchus thornei. This thesis is presented for the degree of Doctor of Philosophy of Murdoch University by Sameer Dilip Khot B.Sc. (Botany) & M.Sc. (Plant Pathology & Mycology), University of Mumbai, India M.S. (Plant Pathology), North Dakota State University, USA Western Australian State Agricultural Biotechnology Centre School of Veterinary and Life Sciences Murdoch University Perth, Western Australia 2018 1 DECLARATION I declare that this thesis is my own account of my research and contains as its main content work which has not previously been submitted for a degree at any tertiary education institution. Signature: Sameer D. Khot Date: 22-01-2018 2 ABSTRACT The root lesion nematode (RLN), Pratylenchus thornei, is a biotrophic migratory pest of plant roots and its infestation causes losses in many economically important crops. RNA interference (RNAi) is a naturally occurring eukaryotic phenomenon and can be used to silence parasitism effector genes of P. thornei using host-mediated RNAi. This may be developed as an environmentally friendly and a cost-effective control strategy. The overall aims of this research were to investigate the effects of in vitro and in planta RNAi silencing of putative P. thornei parasitism effector genes, and their nematicidal effects in two host plants. Five putative target parasitism genes vital for nematode entry into roots (Pt-Eng-1, Pt- PL), feeding (Pt-CLP) and suppressing host defence responses (Pt-UEP, Pt-GST) were identified, validated in silico using comparative bioinformatics, cloned into suitable in vitro transcription and binary vectors, and advanced to RNAi studies. -
Diversity, Phylogeny, Characterization and Diagnostics of Root-Knot and Lesion Nematodes
Diversity, phylogeny, characterization and diagnostics of root-knot and lesion nematodes Toon Janssen Promotors: Prof. Dr. Wim Bert Prof. Dr. Gerrit Karssen Thesis submitted to obtain the degree of doctor in Sciences, Biology Proefschrift voorgelegd tot het bekomen van de graad van doctor in de Wetenschappen, Biologie 1 Table of contents Acknowledgements Chapter 1: general introduction 1 Organisms under study: plant-parasitic nematodes .................................................... 11 1.1 Pratylenchus: root-lesion nematodes ..................................................................................... 13 1.2 Meloidogyne: root-knot nematodes ....................................................................................... 15 2 Economic importance ..................................................................................................... 17 3 Identification of plant-parasitic nematodes .................................................................. 19 4 Variability in reproduction strategies and genome evolution ..................................... 22 5 Aims .................................................................................................................................. 24 6 Outline of this study ........................................................................................................ 25 Chapter 2: Mitochondrial coding genome analysis of tropical root-knot nematodes (Meloidogyne) supports haplotype based diagnostics and reveals evidence of recent reticulate evolution. 1 Abstract -
PCR-RFLP and Sequencing Analysis of Ribosomal DNA of Bursaphelenchus Nematodes Related to Pine Wilt Disease(L)
Fundam. appl. Nemalol., 1998,21 (6), 655-666 PCR-RFLP and sequencing analysis of ribosomal DNA of Bursaphelenchus nematodes related to pine wilt disease(l) Hideaki IvVAHORI, Kaku TSUDA, Natsumi KANZAKl, Katsura IZUI and Kazuyoshi FUTAI Cmduate School ofAgriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan. Accepted for publication 23 December 1997. Summary -A polymerase chain reaction - restriction fragment polymorphism (PCR-RFLP) analysis was used for the discri mination of isolates of Bursaphelenchus nematode. The isolares of B. xylophilus examined originared from Japan, the United Stares, China, and Canada and the B. mucronatus isolates from Japan, China, and France. Ribosomal DNA containing the 5.8S gene, the internai transcribed spacer region 1 and 2, and partial regions of 18S and 28S gene were amplified by PCR. Digestion of the amplified products of each nematode isolate with twelve restriction endonucleases and examination of resulting RFLP data by cluster analysis revealed a significant gap between B. xylophllus and B. mucronatus. Among the B. xylophilus isolares examined, Japanese pathogenic, Chinese and US isolates were ail identical, whereas Japanese non-pathogenic isolares were slightly distinct and Canadian isolates formed a separate cluster. Among the B. mucronalUS isolates, two Japanese isolares were very similar to each other and another Japanèse and one Chinese isolare were identical to each other. The DNA sequence data revealed 98 differences (nucleotide substitutions or gaps) in 884 bp investigated between B. xylophilus isolare and B. mucronmus isolate; DNA sequence data of Aphelenchus avenae and Aphelenchoides fragariae differed not only from those of Bursaphelenchus nematodes, but also from each other. -
A Reappraisal of Tylenchina (Nemata) 1
University of Nebraska - Lincoln DigitalCommons@University of Nebraska - Lincoln Faculty Publications from the Harold W. Manter Laboratory of Parasitology Parasitology, Harold W. Manter Laboratory of 1987 A Reappraisal of Tylenchina (Nemata) 1. For a New Approach to the Taxonomy of Tylenchina Michel Luc Muséun1 national d'Histoire naturelle Armand R. Maggenti University of California - Davis Renaud Fortuner California Department of Food and Agriculture Dewey J. Raski University of California - Davis Etienne Geraert Instituut voor Dierkunde Follow this and additional works at: https://digitalcommons.unl.edu/parasitologyfacpubs Part of the Parasitology Commons Luc, Michel; Maggenti, Armand R.; Fortuner, Renaud; Raski, Dewey J.; and Geraert, Etienne, "A Reappraisal of Tylenchina (Nemata) 1. For a New Approach to the Taxonomy of Tylenchina" (1987). Faculty Publications from the Harold W. Manter Laboratory of Parasitology. 109. https://digitalcommons.unl.edu/parasitologyfacpubs/109 This Article is brought to you for free and open access by the Parasitology, Harold W. Manter Laboratory of at DigitalCommons@University of Nebraska - Lincoln. It has been accepted for inclusion in Faculty Publications from the Harold W. Manter Laboratory of Parasitology by an authorized administrator of DigitalCommons@University of Nebraska - Lincoln. Tribune A REAPPRAISAL OF "IYL,ENCHINA (NEMATA) 1. FOR A NEW APPROACH TO THE TAXONOMY OF TYLENCHINA Michel LUC",Armand R. MAGGENTI**, Renaud FORTUNER***, Dewey J. RASKI** and Etienne GERAERT**** * Muséun1 national d'Histoire naturelle, Laboratoire des Vers, 61, rue de Buffon, 75005 Paris; ** Division of Nematology, University of California, Davis, CA 95616, USA; *** California Department of Food and Agriculture, Analysis and Identification (Nematology), 1220 N. Street, Sacramento, CA 95814, USA, and **** Rijksuniversiteit Gent, Instituut voor Dierkunde, Ledeganckstraat 35, 9000 Gent, Belgium. -
Draft Policy Review
Draft policy review A categorisation of invertebrate and pathogen organisms associated with fresh table grape bunches (Vitis spp.) imported from other Australian states and territories Supporting your success Draft pest categorisation report Contributing authors Bennington JM Research Officer – Biosecurity and Regulation, Plant Biosecurity Hammond NE Research Officer – Biosecurity and Regulation, Plant Biosecurity Hooper RG Research Officer – Biosecurity and Regulation, Plant Biosecurity Jackson SL Research Officer – Biosecurity and Regulation, Plant Biosecurity Poole MC Research Officer – Biosecurity and Regulation, Plant Biosecurity Tuten SJ Senior Policy Officer – Biosecurity and Regulation, Plant Biosecurity Department of Agriculture and Food, Western Australia, December 2014 Document citation DAFWA 2015, Draft policy review: A categorisation of invertebrate and pathogen organisms associated with fresh table grape bunches (Vitis spp.) imported from other Australian states and territories. Department of Agriculture and Food, Western Australia, South Perth. Copyright© Western Australian Agriculture Authority, 2015 Western Australian Government materials, including website pages, documents and online graphics, audio and video are protected by copyright law. Copyright of materials created by or for the Department of Agriculture and Food resides with the Western Australian Agriculture Authority established under the Biosecurity and Agriculture Management Act 2007. Apart from any fair dealing for the purposes of private study, research, -
Western Australian Viticulture Industry Biosecurity Plan
Western Australian Viticulture Industry Biosecurity Plan Version 1.1; August 2017 Contributing Organisations The Western Australian Viticulture Industry Biosecurity Plan was coordinated by the Department of Primary Industries and Regional Development and developed through a partnership approach using government and industry resources and expertise. The development of this plan was made possible by Royalties for Regions Funding. The following organisations and agencies were involved in the review of the plan: • The Department of Primary Industries and Regional Development. • Wines of Western Australia • Table Grapes Western Australia Document citation DPIRD 2017, Western Australian Viticulture Industry Biosecurity Plan, version 1.1. Department of Primary Industries and Regional Development, Western Australia. Copyright © Western Australian Agriculture Authority, 2017 Western Australian Government materials, including website pages, documents and online graphics, audio and video are protected by copyright law. Copyright of materials created by or for the Department of Primary Industries and Regional Development resides with the Western Australian Agriculture Authority established under the Biosecurity and Agriculture Management Act 2007. Apart from any fair dealing for the purposes of private study, research, criticism or review, as permitted under the provisions of the Copyright Act 1968, no part may be reproduced or reused for any commercial purposes whatsoever without prior written permission of the Western Australian Agriculture Authority. -
Diversidad De Nemátodos Fitoparásitos Asociados Al Cultivo De Maíz En El Municipio De Guasave, Sinaloa
INSTITUTO POLITÉCNICO NACIONAL CENTRO INTERDISCIPLINARIO DE INVESTIGACIÓN PARA EL DESARROLLO INTEGRAL REGIONAL UNIDAD SINALOA Diversidad de nemátodos fitoparásitos asociados al cultivo de maíz en el municipio de Guasave, Sinaloa. TESIS QUE PARA OBTENER EL GRADO DE MAESTRÍA EN RECURSOS NATURALES Y MEDIO AMBIENTE PRESENTA ULISES GONZÁLEZ GÜITRÓN GUASAVE, SINALOA; MÉXICO DICIEMBRE DE 2013 I II III RECONOCIMIENTO A PROYECTOS Y BECAS La investigación se realizó en los laboratorios de Nemátodos y Nutrición Vegetal pertenecientes al centro interdisciplinario de Investigación para el Desarrollo Integral Regional Unidad Sinaloa (CIIDIR-SIN) del Instituto Politécnico Nacional (IPN). El trabajo de maestría estuvo asesorado por el Dr. Manuel Mundo Ocampo y el M.C. Jesús Ricardo Camacho Báez, contando con el apoyo otorgado por el Consejo Nacional de Ciencia y Tecnologia CONACyT, con número de CVU 418291. Se agradece el apoyo al programa Institucional de Formación de Investigadores (PIFI) con el proyecto “Búsqueda de nemátodos potencialmente entomopatógenos asociados al cultivo de maíz en el norte de Sinaloa (segunda etapa)” con clave 20131792. Así como el reconocimiento a INAPI SINALOA por su beca de terminación de tesis y a la Organización de Nematólogos de los Trópicos Americanos (ONTA) por su premio económico con el cual pude asistir al congreso número XLIV en Cancún, México. IV DEDICATORIA El presente trabajo está dedicado a dos personas muy especiales que quiero con todo mi corazón, que supieron hacer de mí una persona consiente y con valores. Las cuales me han ayudado a sobrellevar todo tipo de problemas y situaciones. Me refiero a mis padres Joel González Betancourt e Irma María Güitrón Padilla.