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Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK South Tees Hospitals NHS Foundation Trust Issue No: 003 Issue date: 07 December 2020 South Tees Hospitals NHS Contact: Ann Wallis Foundation Trust Tel: +44 (0)1642 850850 Ext 55239 Pathology Services Fax: +44 (0)1642 282636 The James Cook University E-Mail: [email protected] 9210 Hospital Website: www.southtees.nhs.

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Pasteurella Multocida Isolated from Cattle
Journal of Applied Pharmaceutical Science Vol. 3 (04), pp. 106-110, April, 2013 Available online at http://www.japsonline.com DOI: 10.7324/JAPS.2013.3419 ISSN 2231-3354 Antibiotic Susceptibility and Molecular Analysis of Bacterial Pathogen Pasteurella Multocida Isolated from Cattle Azmat Jabeen, Mahrukh Khattak, Shahzad Munir*, Qaiser Jamal, Mubashir Hussain Department of Microbiology, Kohat University of Science and Technology, Kohat, Khyber Pakhtunkhwa, Pakistan. ARTICLE INFO ABSTRACT Article history: Pasteurella multocida is a Gram negative, non motile and coccobacillus bacterium. It has 5 strains i.e. A, B, D, Received on: 01/02/2013 E and F and 16 serotypes (1-16). In present study, we analyzed Pasteurella multocida B: 2 strains, responsible Revised on: 19/02/2013 for Hemorrhagic Septicemia (HS) in cattle, on morphological/microbial, biochemical, molecular level and to Accepted on: 15/03/2013 check the antibiotic sensitivity of the Pasteurella multocida. Microbial analysis showed that while grown on Available online: 27/04/2013 Brain Heart Infusion agar plates and Blood Agar Base Medium, grayish lustrous colonies of Pasteurella multocida were observed. Gram staining showed that Pasteurella multocida are gram negative. Microscopic Key words: observations revealed it to be coccobacillus and it was non- motile. Identification was conducted by Pasteurella multocida, conventional biochemical tests and percentage identification of Analytical Profile Index was 96 %. Antibiotic Hemorrhagic Septicemia, sensitivity with different antibiotics was checked by disk diffusion method and was found resistant to Analytical Profile Index, Augmentin, Amoxicillin and Aztreonam and was more susceptible to Ceftiofur. On molecular level its DNA Antibiotic sensitivity. was extracted and was run with marker having range from 0.5 – 10 kb.
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Physico-Chemical and Bacteriological Quality of Water, And
PHYSICO-CHEMICAL AND BACTERIOLOGICAL QUALITY OF WATER, AND ANTIMICROBIAL SUSCEPTIBILITY OF PATHOGENIC ISOLATES FROM SELECTED WATER SOURCES IN SAMBURU SOUTH. BY JEOPHITA JUNE MWAJUMA (B.Sc, P.G.D.E) REG. NO. I56/7341/02 DEPARTMENT OF PLANT AND MICROBIAL SCIENCES A thesis submitted in partial fulfillment of the requirements for the award of the degree of Master of Science (Microbiology) in the School of Pure and Applied Sciences, Kenyatta University April 2010 ii DECLARATION I, Jeophita June Mwajuma, declare that this thesis is my original work and has not been presented for the award of a degree in any other University or any other award. Signature…………………………………... Date………………………….. We confirm that the work reported in this thesis was carried out by the candidate under our supervision. SUPERVISORS: Prof. Paul Okemo Department of Plant and Microbial Sciences Kenyatta University Signature…………………………………... Date………………………….. Dr. Alexander Njue Department of Plant and Microbial Sciences Kenyatta University Signature…………………………………... Date………………………….. Prof. Kiplagat Kotut Department of Plant and Microbial Sciences Kenyatta University Signature…………………………………... Date………………………….. iii DEDICATION For my girls, Neema and Wema. Babies, the sky is the limit! iv Formatted: Centered, Indent: Left: 0.25", 1. ACKNOWLEDGEMENT No bullets or numbering I wish to thank my supervisors Prof. Paul Okemo, Dr. Alexander Njue and Prof. Kiplagat Kotut for their expert advice and encouragement throughout the period of this study. My gratitude also goes to Earthwatch Institute for funding my research work through the Samburu Communities, Water and Wildlife project. I also wish to thank KEMRI, Welcome Trust Laboratories Kilifi, Wamba Mission Hospital and Mombasa Polytechnic University College for providing me with laboratory space and analytical tools.
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Resistant Gram-Negative Bacteria in Urine of Pregnant Women Attending Antenatal Clinic of Mother and Child Hospital, Ondo, Nigeria
Vol. 15(5), pp. 209-216, May, 2021 DOI: 10.5897/AJMR2021.9491 Article Number: FAE2CD666760 ISSN: 1996-0808 Copyright ©2021 Author(s) retain the copyright of this article African Journal of Microbiology Research http://www.academicjournals.org/AJMR Full Length Research Paper Phenotypic and molecular characterization of multiple- resistant gram-negative bacteria in urine of pregnant women attending antenatal clinic of Mother and Child hospital, Ondo, Nigeria 1 1 2* Eunice Damilola Wilkie , Anthonia Olufunke Oluduro , Thonda Oluwakemi Abike and Chidinma Vivian Chukwudum1 1Department of Microbiology, Faculty of Sciences, Obafemi Awolowo University, Ile-Ife, Osun State, Nigeria. 2Department of Biological Sciences, Faculty of Sciences, Kings University, Odeomu, Osun State, Nigeria. Received 27 January, 2021; Accepted 12 March, 2021 Phenotypic and molecular characterization of multiple antibiotic resistant Gram-negative bacteria in urine samples of pregnant women in Mother and Child Hospital, Nigeria was reported. In the study, 407 apparently healthy pregnant women were recruited. Structured questionnaire was administered to the patients to obtain their socio-demographic information and the medical history. Urine samples were collected, processed and analysed using standard microbiological procedures. Detailed identification of the bacteria isolates was done using biochemical characterization using Bergey’s Manual of Determinative Bacteriology and Analytical Profile Index (API) Kit. The antimicrobial susceptibility testing of the bacteria isolates was carried out using the Kirby-Bauer’s disk diffusion technique. Detection of the beta lactamase resistance genes (bla CTX-M and Tet A) was done by polymerase chain reactions (PCR) with appropriate primers. The following Gram-negative bacteria were recovered comprising Pseudomonas aeruginosa 48 (34.0%), Escherichia coli 30 (21.3%), Klebsiella sp.
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Characterization, Prevalance and Antimicrobial Susceptibility Pattern
International Journal of Clinical Obstetrics and Gynaecology 2018; 2(6): 31-42 ISSN (P): 2522-6614 ISSN (E): 2522-6622 © Gynaecology Journal Characterization, prevalance and antimicrobial www.gynaecologyjournal.com 2018; 2(6): 31-42 susceptibility pattern of bacterial uropathogens isolated Received: 21-09-2018 Accepted: 24-10-2018 from pregnant women at Lahore general hospital, Lahore, Pakistan Rabia Habib Institute of Molecular Biology and Biotechnology, University of Lahore, Pakistan Rabia Habib, Muhammad Danish Mehmood, Sana Noreen, Huma Anwar, Mehreen Gul, Nazia Ayub and Almas Raza Muhammad Danish Mehmood Ottoman Pharma (Immuno Division), Raiwind Road Lahore, Abstract Pakistan Urinary tract infection (UTI) is common in ladies living in developing countries which may progress to complications such as pyelonephritis and preterm delivery during pregnancy. The present study provides an Sana Noreen insight for causative agent of UTI, their prevalence in pregnant ladies and its association with age, Ottoman Pharma (Immuno metabolic disorder and gestational period. Total of 375 midstream samples were collected from pregnant Division), Raiwind Road Lahore, women, pure culture were segregated on selective media and identified through analytical profile index Pakistan (API) to evaluate prevalence of uropathogens in UTI and ASB patients. Isolated uropathogenic E. coli were further characterized by polymerase chain reaction (PCR) using specific primers for genotype cjrA, cjrB, Huma Anwar and cjrC. Among 375 midstream urine samples of pregnant women, 160 cases of UTI and ASB (≥105 Ottoman Pharma (Immuno CFU) were recorded. API analysis of such samples showed 65(40.6%), 55(34.35%) and 40(25%) of E. coli, Division), Raiwind Road Lahore, Pakistan Enterococci and Staphylococci respectively.
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Rapid Identification and Antimicrobial Susceptibility Profiling of Anaerobic Bacteria in Clinical Specimens
Egyptian Journal of Medical Microbiology, July 2012 Vol. 21, No. 3 Rapid Identification and Antimicrobial Susceptibility Profiling of Anaerobic Bacteria in Clinical Specimens *Wafaa N. El-tayab, **Fatma Alzhraa M. Gomaa *Associate Professor of Microbiology & Immunology, Faculty of Pharmacy Misr International University, Cairo, Egypt **Associate Professor of Microbiology & Immunology, Faculty of Pharmacy for girls- Al-Azhar University, Egypt ABSTRACT Objective: The aim of the current study was to evaluate the ability of API 20A, and Microscan Rapid- Anaerobe identification system to identify a spectrum of clinically significant anaerobic isolates against the gold standard conventional method. Also to determine the antimicrobial susceptibility of anaerobic isolates using different methods. Materials and Methods: Anaerobes were isolated from the different clinical specimens and all isolates were initially identified by conventional tests. Identification of isolates were made also by using the API 20A and Microscan systems. Antimicrobial susceptibility of all isolates were determined by disc diffusion and broth-disk method and compared to MIC determined by broth dilution method. Results: A total of 51 isolates were recovered from clinical specimens. API 20A and Microscan correctly identified 70.6% and 82.4% of strains to species level respectively. Eight strains were misidentified by API 20A and 9 were misidentified by Microscan. B. fragilis group isolates were the most encountered clinically significant isolates among the gram-negative anaerobes. Penicillin and ampicillin had poor activity against B. fragilis group, Prevotella spp. Eubacterium spp. and Veillonella spp. Members of the B. fragilis group were more resistant to cefoxitin, than other anaerobes (66.6%). Resistance to clindamycin varied among the species range from 11.1% to 50%.
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Pdf 1032003 6
Peer-Reviewed Journal Tracking and Analyzing Disease Trends pages 1891–2100 EDITOR-IN-CHIEF D. Peter Drotman Managing Senior Editor EDITORIAL BOARD Polyxeni Potter, Atlanta, Georgia, USA Dennis Alexander, Addlestone Surrey, United Kingdom Senior Associate Editor Barry J. Beaty, Ft. Collins, Colorado, USA Brian W.J. Mahy, Atlanta, Georgia, USA Martin J. Blaser, New York, New York, USA Christopher Braden, Atlanta, GA, USA Associate Editors Carolyn Bridges, Atlanta, GA, USA Paul Arguin, Atlanta, Georgia, USA Arturo Casadevall, New York, New York, USA Charles Ben Beard, Ft. Collins, Colorado, USA Kenneth C. Castro, Atlanta, Georgia, USA David Bell, Atlanta, Georgia, USA Thomas Cleary, Houston, Texas, USA Charles H. Calisher, Ft. Collins, Colorado, USA Anne DeGroot, Providence, Rhode Island, USA Michel Drancourt, Marseille, France Vincent Deubel, Shanghai, China Paul V. Efﬂ er, Perth, Australia Ed Eitzen, Washington, DC, USA K. Mills McNeill, Kampala, Uganda David Freedman, Birmingham, AL, USA Nina Marano, Atlanta, Georgia, USA Kathleen Gensheimer, Cambridge, MA, USA Martin I. Meltzer, Atlanta, Georgia, USA Peter Gerner-Smidt, Atlanta, GA, USA David Morens, Bethesda, Maryland, USA Duane J. Gubler, Singapore J. Glenn Morris, Gainesville, Florida, USA Richard L. Guerrant, Charlottesville, Virginia, USA Patrice Nordmann, Paris, France Scott Halstead, Arlington, Virginia, USA Tanja Popovic, Atlanta, Georgia, USA David L. Heymann, Geneva, Switzerland Jocelyn A. Rankin, Atlanta, Georgia, USA Daniel B. Jernigan, Atlanta, Georgia, USA Didier Raoult, Marseille, France Charles King, Cleveland, Ohio, USA Pierre Rollin, Atlanta, Georgia, USA Keith Klugman, Atlanta, Georgia, USA Dixie E. Snider, Atlanta, Georgia, USA Takeshi Kurata, Tokyo, Japan Frank Sorvillo, Los Angeles, California, USA S.K. Lam, Kuala Lumpur, Malaysia David Walker, Galveston, Texas, USA Bruce R.
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API 20E Manufacture Instructions
REF 20 100 / 20 160 07584D - GB - 2002/10 ® IVD 20 E Identification system for Enterobacteriaceae and other non-fastidious Gram-negative rods SUMMARY AND EXPLANATION Material : API 20 E is a standardized identification system for - Pipettes or PSIpettes Enterobacteriaceae and other non-fastidious, Gram- - Ampule protector negative rods which uses 21 miniaturized biochemical - Ampule rack tests and a database. The complete list of those - General microbiology laboratory equipment organisms that it is possible to identify with this system is given in the Identification Table at the end of this package POSSIBLE ADDITIONAL REAGENTS : insert. - API OF Medium (Ref. 50 110) : Test for the determination of fermentative or oxidative PRINCIPLE metabolism. The API 20 E strip consists of 20 microtubes containing - API M Medium (Ref. 50 120) : dehydrated substrates. These tests are inoculated with Test for motility of facultative anaerobic bacteria. a bacterial suspension that reconstitutes the media. During incubation, metabolism produces color changes WARNINGS AND PRECAUTIONS that are either spontaneous or revealed by the addition of • For in vitro diagnostic use and microbiological reagents. control. The reactions are read according to the Reading Table • For professional use only. and the identification is obtained by referring to the • This kit contains products of animal origin. Certified Analytical Profile Index or using the identification software. knowledge of the origin and/or sanitary state of the animals does not totally guarantee the absence of CONTENT OF THE KIT transmissible pathogenic agents. It is therefore Kit for 25 tests (ref. 20 100) recommended that these products be treated as - 25 API 20 E strips potentially infectious, and handled observing the usual - 25 incubation boxes safety precautions (do not ingest or inhale).
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Biochemical Characterization and Identification of Bacterial Strains
Biochemical characterization and identification of bacterial strains isolated from drinking water sources of Kohat, Pakistan Tassadaq Hussain, Aneela Roohi, Shehzad Munir, Iftikhar Ahmed, Jafar Khan, Veronique Edel-Hermann, Kil Yong Kim, Muhammad Anees To cite this version: Tassadaq Hussain, Aneela Roohi, Shehzad Munir, Iftikhar Ahmed, Jafar Khan, et al.. Biochemical characterization and identification of bacterial strains isolated from drinking water sources ofKohat, Pakistan. African Journal of Microbiology Research, Academic Journal, 2013, 7 (16), pp.1579-1590. 10.5897/AJMR12.2204. hal-01005074 HAL Id: hal-01005074 https://hal.archives-ouvertes.fr/hal-01005074 Submitted on 29 May 2020 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Vol. 7(16), pp. 1579-1590, 16 April, 2013 DOI: 10.5897/AJMR12.2204 ISSN 1996-0808 ©2013 Academic Journals African Journal of Microbiology Research http://www.academicjournals.org/AJMR Full Length Research Paper Biochemical characterization and identification of bacterial strains isolated from drinking water sources of Kohat, Pakistan Tassadaq Hussain1, Aneela Roohi1, Shehzad Munir1, Iftikhar Ahmed2, Jafar Khan1, Veronique Edel-Hermann3, Kil Yong Kim4 and Muhammad Anees1* 1Department of Microbiology, Kohat University of Science and Technology, Kohat, Pakistan.
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Factors Affecting Experimental Streptococcus Agalactiae Infection in Tilapia, Oreochromis Niloticus
View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Stirling Online Research Repository FACTORS AFFECTING EXPERIMENTAL STREPTOCOCCUS AGALACTIAE INFECTION IN TILAPIA, OREOCHROMIS NILOTICUS THESIS SUBMITTED TO THE UNIVERSITY OF STIRLING FOR THE DEGREE OF DOCTOR OF PHILOSOPHY BY DILOK WONGSATHEIN 27 SEPTEMBER 2012 INSTITUTE OF AQUACULTURE Declaration Declaration I declare that this thesis has been composed in its entirety by me. Except where specifically acknowledged, the work described in this thesis has been conducted by me and has not been submitted for any other degree. Signature: _________________________________________ Signature of supervisor: _________________________________________ Date: _________________________________________ II Abstract Abstract Streptococcus agalactiae infection is one of the major disease problems affecting farmed tilapia (Oreochromis niloticus) worldwide. Tilapia are highly susceptible to this disease which results in mortality of up to 70% over a period of around 7 days and significant economic losses for farmers. Affected tilapia commonly present with an irregular behaviour associated with meningoencephalitis and septicaemia. Currently, factors affecting the virulence and transmission of S. agalactiae in fish including tilapia are poorly understood. Reports from natural outbreaks of S. agalactiae infection on tilapia farms have suggested larvae and juvenile or fish smaller than 20 g are not susceptible. In addition, there is variability in individual response to experimental inflammatory challenge associated with coping styles (bold, shy) in common carp (Cyprinus carpio). The central hypotheses of this thesis were that weight, age and coping style might affect the development and progression of this bacterial disease. This study investigated these three factors with experimental S. agalactiae infection in Nile tilapia.
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Antimicrobial Sensitivity Pattern of Urine Isolates from Asymptomatic Bacteriuria During Pregnancy
E:/Biomedica Vol.22 Jan. – Jun. 2006/Bio-9 (A) ANTIMICROBIAL SENSITIVITY PATTERN OF URINE ISOLATES FROM ASYMPTOMATIC BACTERIURIA DURING PREGNANCY 1AZIZ MARJAN KHATTAK, 2HABIB-ULLAH KHAN, 3IHSAN-ULLAH MASHUD 4BUSHRA ASHIQ AND 5SYED HUMAYUN SHAH Departments of 1,5 Pathology, 2,3 Medicine, Gomal Medical College, Dera Ismail Khan – Pakistan 4Departments of Microbiology, BMSI, JPMC, Karachi Screening women for asymptomatic bacteriuria (ASB) on the first antenatal visit is a part of standard obstetric care. Treating women with ASB decreases the chances of maternal and foetal complications. This study was conducted to find out the spectrum of urine pathogens and their drug susceptibility pattern for ASB during pregnancy. The study was conducted in the Basic Medical Sciences Institute, Jinnah Postgraduate Medical Centre Karachi, from September 2001 to March 2002. Two hundred and ninety women, apparently normal with confirmed pregnancy, were registered. A voided midstream urine specimen was collected and cultured. A significant growth i.e. ≥10 5 organisms/ml was identified with Analytical Profile Index 20 tests for identification of Enterobacteraceae (API-20-E) and for Gram positive cocci by other standard methods. The prevalence of ASB was found 6.2%. Antimicrobial sensitivity was determined by disc diffusion Kirby Bauyer method after matching the turbidity with 0.5 McFarland ,s standard. Most of the recommended drugs were found to have encouraging results, however, Escherichia coli showed 66.67% resistance to ampicillins and sulphonamides. Enterobacters showed 100% resistance to ampicillins, cephalosporins and nitrofurantoin. Staphylococcus saprophyticus showed 66.67% resistance to ampicillins and sulphonamides. It was concluded that detection of ASB during pregnancy and appropriate use of antimicrobials is only possible after culture of urine.
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Medical Microbiology Manual
LABORATORY EXERCISES to accompany MICROBIOLOGY LABORATORY BSL 214 Professor Susan C. Kavanaugh Bluegrass Community & Technical College 2010 INTRODUCTION TO CULTURING, MEDIA, AND ASEPTIC TECHNIQUES Even though you may be starting a career in a health-related field, you may be unaware of the number and variety of microorganisms (microbes) found everywhere in our environment, including the human body. In this laboratory you will learn new techniques and observations which relate to the concepts of microbiology and to your future health career. Most of the microorganisms that you will use in these laboratories are normal inhabitants of our environment. These microbes are called normal microbiota for the environment in which they normally reside. Health professionals need this knowledge in order to be able to distinguish normal flora from a possible infectious agent when interpreting microbiological reports. They also need to understand how normal flora can occasionally cause an infection when they invade a different area of the body, or when the patient's immune responses have been compromised. Microorganisms are found almost everywhere. In these first laboratory exercises you will be introduced to aseptic techniques, the procedures followed by microbiologists and healthcare workers to prevent contamination of cultures from outside sources and to prevent introduction of potentially disease-causing microbes (pathogens) into the human body. The methods for handling previously sterilized materials, for taking samples, for handling cultures, and for disposing contaminated materials are all designed to prevent the spread of microbes from one area to another. Pay close attention to the details in the written procedures and to the instructor's demonstrations to prevent contamination of your cultures, yourself, your environment, and the other people in your laboratory as well as prevention of infecting people outside of the laboratory, such as your friends and family.
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