Pasteurella Multocida Isolated from Cattle
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Rapid Identification of Clinical Isolates of Klebsiella Pneumoniae Using MALDI-TOF MS from North India
Bulletin of Pure and Applied Sciences Print version ISSN 0970 0765 Vol.39A (Zoology), No.1, Online version ISSN 2320 3188 January-June 2020: P.194-199 DOI 10.5958/2320-3188.2020.00022.4 Original Research Article Available online at www.bpasjournals.com Rapid Identification of Clinical Isolates of Klebsiella pneumoniae using MALDI-TOF MS from North India 1Sunil Kumar Abstract: 2Zainab Saifi Infections caused by multi-drug resistant (MDR) 3Anil Kumar Sharma Klebsiella pneumoniae are increasing day by day. K. pneumoniae poses a severe public health concern 4 * Sushil Kumar Upadhyay and causes wide array of healthcare associated infections with limited treatment options. The Author’s Affiliation: quick detection of these isolates is of prime 1,2,3,4 Department of Biotechnology, Maharishi importance for the adoption of proper antibiotic Markandeshwar (Deemed to be University), treatment and control measures. Also the mis- Mullana-Ambala, Haryana 133207, India identification using standard lab-based methods is quite common and hence, the clinical *Corresponding author: significance of K. pneumoniae complex members is Dr. Sushil Kumar Upadhyay, inaccurately defined. Here, authors evaluated the Assistant Professor, Department of potential of MALDI-TOF (Matrix-Assisted Laser Biotechnology, Maharishi Markandeshwar Desorption Ionization-Time of Flight) MS (Mass (Deemed to be University), Mullana-Ambala, Spectrometry) to discriminate and quick Haryana 133207, India identification K. pneumoniae complex members. Thus, this study aimed at full MALDI-based E-mail: approach to rapidly detect the K. [email protected] pneumoniae isolates up to species level. We also ORCID: https://orcid.org/0000-0002-1229-4275 explored the antibiotic resistance profile of K. -
Characterization and Antibiotic Sensitivity Profile of Bacteria Isolated from Patients with Respiratory Tract Infections in Bangladesh
Characterization and Antibiotic Sensitivity Profile of Bacteria Isolated from Patients with Respiratory Tract Infections in Bangladesh Shukla Promite1, Sajal K. Saha2, Sunjukta Ahsan1 and Marufa Zerin Akhter1 1Department of Microbiology, University of Dhaka, Dhaka, Bangladesh 2Department of General Practice, Monash University, Building 1, 270 Ferntree Gully Road, Notting Hill VIC 3168, Australia (Received: October 08, 2017; Accepted: December 15, 2017; Published (web): December 23, 2017) ABSTRACT: The study was aimed to characterize bacterial isolates from respiratory tract infections (RTI) and investigate their antibiotic sensitivity profile. Selective media and biochemical tests were used to characterize 40 bacterial isolates. Antibiotic sensitivity testing was conducted using Kirby-Bauer disc diffusion method. About 42.5% (17) RTI patients were infected by Klebsiella pneumoniae, 30% (12) by Escherichia coli and 27.5% (11) by Pseudomonas aeruginosa with no significant gender variation (p-value <0.578). Overall, 47% (out of 20) antibiotics were sensitive, whereas 48% were resistant. Surprisingly, 18% P. aeruginosa and 20% K. pneumoniae were carbapenem-resistant and 4 out of 7 cephalosporin antibiotics were highly resistant irrespective of pathogens. E. coli showed better sensitivity to nitrofurantoin (78%) and levofloxacin (89%), while K. pneumoniae was insensitive to cotrimoxazole (88%), gentamycin (77%) and piperacillin/tazobactam (66%). On the other hand, P. aeruginosa did not respond to P. aeruginosa to nalidixic acid (60%) and ciprofloxacin (60%). This study concludes that nitrofurantoin, levofloxacin, cotrimoxazole, gentamycin and piperacillin/tazobactam antibiotics could be better alternative in treating bacterial RTIs. Key words: Antibiotic sensitivity, bacterial pathogens, RTIs, Bangladesh. INTRODUCTION Antibiotic resistance (AR) is a global public The rise of AR in Bangladesh is probably due to 1 health concern. -
Redalyc.Establishment of a Pathogenicity Index for One-Day-Old
Revista Brasileira de Ciência Avícola ISSN: 1516-635X [email protected] Fundação APINCO de Ciência e Tecnologia Avícolas Brasil Pilatti, RM; Furian, TQ; Lima, DA; Finkler, F; Brito, BG; Salle, CTP; Moraes, HLS Establishment of a Pathogenicity Index for One-day-old Broilers to Pasteurella multocida Strains Isolated from Clinical Cases in Poultry and Swine Revista Brasileira de Ciência Avícola, vol. 18, núm. 2, abril-junio, 2016, pp. 255-260 Fundação APINCO de Ciência e Tecnologia Avícolas Campinas, Brasil Available in: http://www.redalyc.org/articulo.oa?id=179746750008 How to cite Complete issue Scientific Information System More information about this article Network of Scientific Journals from Latin America, the Caribbean, Spain and Portugal Journal's homepage in redalyc.org Non-profit academic project, developed under the open access initiative Brazilian Journal of Poultry Science Revista Brasileira de Ciência Avícola Establishment of a Pathogenicity Index for One-day- ISSN 1516-635X May - Jun 2016 / v.18 / n.2 / 255-260 old Broilers to Pasteurella multocida Strains Isolated http://dx.doi.org/10.1590/1806-9061-2015-0089 from Clinical Cases in Poultry and Swine Author(s) ABSTracT Pilatti RMI Although Pasteurella multocida is a member of the respiratory Furian TQI microbiota, under some circumstances, it is a primary agent of diseases Lima DAI , such as fowl cholera (FC), that cause significant economic losses. Finkler FII Brito BGII Experimental inoculations can be employed to evaluate the pathogenicity Salle CTPI of strains, but the results are usually subjective and knowledge on the Moraes HLSI pathogenesis of this agent is still limited. -
ﺑﺴﻢ اﷲ اﻟﺮﺣﻤﻦ اﻟﺮﺣﻴﻢ Molecular Characterization Of
View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by KhartoumSpace ﺑﺴﻢ اﷲ اﻟﺮﺣﻤﻦ اﻟﺮﺣﻴﻢ Molecular Characterization of Pasteurella multocida Vaccine Strains By: Hajir Badawi Mohammed Ahmed B.V.M Khartoum University (2006) Supervisor: Dr. Awad A. Ibrahim A dissertation submitted to the University of Khartoum in partial fulfillment of the requirements for the degree of M. Sc. in Microbiology Department of Microbiology, Faculty of Veterinary Medicine, University of Khartoum June, 2010 Dedication To my mother Father Brother, sister and friends With great love Acknowledgments First and foremost, I would like to thank my Merciful Allah, the most beneficent for giving me strength and health to accomplish this work. Then I would like to deeply thank my supervisor Dr. Awad A. Ibrahim for his advice, continuous encouragement and patience throughout the period of this work. My gratitude is also extended to prof. Mawia M. Mukhtar and for Dr. Manal Gamal El-dein, Institute of Endemic Disease. My thanks extend to members of Department of Microbiology Faculty of Veterinary Medicine for unlimited assistant and for staff of Central Laboratory Soba. I am grateful to my family for their continuous support and standing beside me all times. My thanks also extended to all whom I didn’t mention by name and to the forbearance of my friends, and colleagues who helped me. Finally I am indebted to all those who helped me so much to make this work a success. Abstract The present study was carried out to study the national haemorrhagic septicaemia vaccine strains at their molecular level. -
Identification of Pasteurella Species and Morphologically Similar Organisms
UK Standards for Microbiology Investigations Identification of Pasteurella species and Morphologically Similar Organisms Issued by the Standards Unit, Microbiology Services, PHE Bacteriology – Identification | ID 13 | Issue no: 3 | Issue date: 04.02.15 | Page: 1 of 28 © Crown copyright 2015 Identification of Pasteurella species and Morphologically Similar Organisms Acknowledgments UK Standards for Microbiology Investigations (SMIs) are developed under the auspices of Public Health England (PHE) working in partnership with the National Health Service (NHS), Public Health Wales and with the professional organisations whose logos are displayed below and listed on the website https://www.gov.uk/uk- standards-for-microbiology-investigations-smi-quality-and-consistency-in-clinical- laboratories. SMIs are developed, reviewed and revised by various working groups which are overseen by a steering committee (see https://www.gov.uk/government/groups/standards-for-microbiology-investigations- steering-committee). The contributions of many individuals in clinical, specialist and reference laboratories who have provided information and comments during the development of this document are acknowledged. We are grateful to the Medical Editors for editing the medical content. For further information please contact us at: Standards Unit Microbiology Services Public Health England 61 Colindale Avenue London NW9 5EQ E-mail: [email protected] Website: https://www.gov.uk/uk-standards-for-microbiology-investigations-smi-quality- and-consistency-in-clinical-laboratories UK Standards for Microbiology Investigations are produced in association with: Logos correct at time of publishing. Bacteriology – Identification | ID 13 | Issue no: 3 | Issue date: 04.02.15 | Page: 2 of 28 UK Standards for Microbiology Investigations | Issued by the Standards Unit, Public Health England Identification of Pasteurella species and Morphologically Similar Organisms Contents ACKNOWLEDGMENTS ......................................................................................................... -
Antibiotic Susceptibility Pattern of Bacterial Uropathogens Isolated
ANTIBIOTIC SUSCEPTIBILITY PATTERN OF BACTERIAL UROPATHOGENS ISOLATED FROM PATIENTS IN NAKURU LEVEL 5 HOSPITAL, KENYA GEORGE TIBI GACHUHI (B.Sc.) I56/CE/20963/2010 A Thesis Submitted in Partial Fulfillment of the Requirements for the Award of the Degree of Master of Science (Microbiology) in the School of Pure and Applied Sciences of Kenyatta University JULY, 2017 ii DECLARATION This thesis is my own original work and has not been presented for the award of a degree in any other university. George Tibi Gachuhi Signature…………………………. Date………………….. Department of Microbiology APPROVAL BY SUPERVISORS This thesis has been submitted for examination with our approval as University supervisors. Dr John Maingi Signature………………………………… Date…………………………………. Department of Microbiology Kenyatta University Dr. Anthony Kebira Signature…………………………………………Date…………………………. Department of Microbiology Kenyatta University iii DEDICATION This research project is dedicated to my beloved wife Salome Tibi and our beloved children Victor, Grace, Martha, Daisy, Caleb and Ann for their support, encouragement and prayer during my time of my study. iv ACKNOWLEDGEMENTS I thank God for His love, guidance and wisdom to be able to successfully complete my research project. I would like to appreciate a number of people who helped me during the preparation and writing of this research project especially Dr. John Maingi and Dr. Anthony Kebira of Kenyatta University, Department of Microbiology. Thank you for your advice and guidance. They have not only been outstanding supervisors, but credible role models. Their guidance, support and encouragement right from the proposal development to the final report would not have been successfully completed without them. I am greatly indebted to the staff at Nakuru Level 5 Hospital especially Dr. -
Clinical Antibiotic Guidelines†
CLINICAL ANTIBIOTIC GUIDELINES† ACYCLOVIR IV*/PO *RESTRICTED TO ANTIBIOTIC FORM Predictable activity: Unpredictable activity: No activity: Herpes Simplex Cytomegalovirus Epstein Barr Virus Herpes Zoster Indicated: IV: 1. Therapy for suspected or documented Herpes simplex encephalitis 2. Therapy for suspected or documented Herpes simplex infection of a newborn or immunocompromised patient 3. Therapy for primary varicella infection in immunocompromised patients 4. Therapy for severe or disseminated varicella-zoster infections in immunocompromised or immunocompetent patient 5. Therapy for primary genital herpes with neurologic complications Oral: 1. Therapy for primary Herpes simplex infections (oral/genital) 2. Suppressive (preventative) therapy for recurrent (³ 6 episodes/year) severe Herpes simplex infections (oral/genital) 3. Episodic therapy for recurrent (³ 6 episodes/year) Herpes simplex genital infections (initiate within 24 hours of prodrome onset) 4. Prophylaxis for HSV in bone marrow transplants where patient is seropositive 5. Therapy and suppressive therapy for Eczema Herpeticum 6. Therapy for varicella-zoster infections in immunocompetent and immunocompromised patients (if not severe) 7. Therapy for primary varicella infections in pregnancy 8. Therapy for varicella in immunocompetent patients > 13 years old (initiate within 24 hours of rash onset) 9. Therapy for varicella in patients < 13 years old (initiate within 24 hours of rash onset) if there is a chronic cutaneous or pulmonary disorder, long term salicylate therapy, or short, intermittent or aerosolized corticosteroid use Not Indicated: 1. Therapy for acute Epstein-Barr infections (acute mononucleosis) 2. Therapy for documented CMV infections CLINICAL ANTIBIOTIC GUIDELINES† AMIKACIN RESTRICTED TO ANTIBIOTIC FORM Predictable activity: Unpredictable activity: No activity: Enterobacteriaceae Staphylococcus spp Streptococcus spp Pseudomonas spp Enterococcus spp some Mycobacterium spp Alcaligenes spp Anaerobes Indicated: 1. -
Physico-Chemical and Bacteriological Quality of Water, And
PHYSICO-CHEMICAL AND BACTERIOLOGICAL QUALITY OF WATER, AND ANTIMICROBIAL SUSCEPTIBILITY OF PATHOGENIC ISOLATES FROM SELECTED WATER SOURCES IN SAMBURU SOUTH. BY JEOPHITA JUNE MWAJUMA (B.Sc, P.G.D.E) REG. NO. I56/7341/02 DEPARTMENT OF PLANT AND MICROBIAL SCIENCES A thesis submitted in partial fulfillment of the requirements for the award of the degree of Master of Science (Microbiology) in the School of Pure and Applied Sciences, Kenyatta University April 2010 ii DECLARATION I, Jeophita June Mwajuma, declare that this thesis is my original work and has not been presented for the award of a degree in any other University or any other award. Signature…………………………………... Date………………………….. We confirm that the work reported in this thesis was carried out by the candidate under our supervision. SUPERVISORS: Prof. Paul Okemo Department of Plant and Microbial Sciences Kenyatta University Signature…………………………………... Date………………………….. Dr. Alexander Njue Department of Plant and Microbial Sciences Kenyatta University Signature…………………………………... Date………………………….. Prof. Kiplagat Kotut Department of Plant and Microbial Sciences Kenyatta University Signature…………………………………... Date………………………….. iii DEDICATION For my girls, Neema and Wema. Babies, the sky is the limit! iv Formatted: Centered, Indent: Left: 0.25", 1. ACKNOWLEDGEMENT No bullets or numbering I wish to thank my supervisors Prof. Paul Okemo, Dr. Alexander Njue and Prof. Kiplagat Kotut for their expert advice and encouragement throughout the period of this study. My gratitude also goes to Earthwatch Institute for funding my research work through the Samburu Communities, Water and Wildlife project. I also wish to thank KEMRI, Welcome Trust Laboratories Kilifi, Wamba Mission Hospital and Mombasa Polytechnic University College for providing me with laboratory space and analytical tools. -
Resistant Gram-Negative Bacteria in Urine of Pregnant Women Attending Antenatal Clinic of Mother and Child Hospital, Ondo, Nigeria
Vol. 15(5), pp. 209-216, May, 2021 DOI: 10.5897/AJMR2021.9491 Article Number: FAE2CD666760 ISSN: 1996-0808 Copyright ©2021 Author(s) retain the copyright of this article African Journal of Microbiology Research http://www.academicjournals.org/AJMR Full Length Research Paper Phenotypic and molecular characterization of multiple- resistant gram-negative bacteria in urine of pregnant women attending antenatal clinic of Mother and Child hospital, Ondo, Nigeria 1 1 2* Eunice Damilola Wilkie , Anthonia Olufunke Oluduro , Thonda Oluwakemi Abike and Chidinma Vivian Chukwudum1 1Department of Microbiology, Faculty of Sciences, Obafemi Awolowo University, Ile-Ife, Osun State, Nigeria. 2Department of Biological Sciences, Faculty of Sciences, Kings University, Odeomu, Osun State, Nigeria. Received 27 January, 2021; Accepted 12 March, 2021 Phenotypic and molecular characterization of multiple antibiotic resistant Gram-negative bacteria in urine samples of pregnant women in Mother and Child Hospital, Nigeria was reported. In the study, 407 apparently healthy pregnant women were recruited. Structured questionnaire was administered to the patients to obtain their socio-demographic information and the medical history. Urine samples were collected, processed and analysed using standard microbiological procedures. Detailed identification of the bacteria isolates was done using biochemical characterization using Bergey’s Manual of Determinative Bacteriology and Analytical Profile Index (API) Kit. The antimicrobial susceptibility testing of the bacteria isolates was carried out using the Kirby-Bauer’s disk diffusion technique. Detection of the beta lactamase resistance genes (bla CTX-M and Tet A) was done by polymerase chain reactions (PCR) with appropriate primers. The following Gram-negative bacteria were recovered comprising Pseudomonas aeruginosa 48 (34.0%), Escherichia coli 30 (21.3%), Klebsiella sp. -
Interaction Study of Pasteurella Multocida with Culturable Aerobic
Hanchanachai et al. BMC Microbiology (2021) 21:19 https://doi.org/10.1186/s12866-020-02071-4 RESEARCH ARTICLE Open Access Interaction study of Pasteurella multocida with culturable aerobic bacteria isolated from porcine respiratory tracts using coculture in conditioned media Nonzee Hanchanachai1,2, Pramote Chumnanpuen2,3 and Teerasak E-kobon2,4,5* Abstract Background: The porcine respiratory tract harbours multiple microorganisms, and the interactions between these organisms could be associated with animal health status. Pasteurella multocida is a culturable facultative anaerobic bacterium isolated from healthy and diseased porcine respiratory tracts. The interaction between P. multocida and other aerobic commensal bacteria in the porcine respiratory tract is not well understood. This study aimed to determine the interactions between porcine P. multocida capsular serotype A and D strains and other culturable aerobic bacteria isolated from porcine respiratory tracts using a coculture assay in conditioned media followed by calculation of the growth rates and interaction parameters. Results: One hundred and sixteen bacterial samples were isolated from five porcine respiratory tracts, and 93 isolates were identified and phylogenetically classified into fourteen genera based on 16S rRNA sequences. Thirteen isolates from Gram-negative bacterial genera and two isolates from the Gram-positive bacterial genus were selected for coculture with P. multocida. From 17 × 17 (289) interaction pairs, the majority of 220 pairs had negative interactions indicating competition for nutrients and space, while 17 pairs were identified as mild cooperative or positive interactions indicating their coexistence. All conditioned media, except those of Acinetobacter, could inhibit P. multocida growth. Conversely, the conditioned media of P. multocida also inhibited the growth of nine isolates plus themselves. -
Metaproteomics Characterization of the Alphaproteobacteria
Avian Pathology ISSN: 0307-9457 (Print) 1465-3338 (Online) Journal homepage: https://www.tandfonline.com/loi/cavp20 Metaproteomics characterization of the alphaproteobacteria microbiome in different developmental and feeding stages of the poultry red mite Dermanyssus gallinae (De Geer, 1778) José Francisco Lima-Barbero, Sandra Díaz-Sanchez, Olivier Sparagano, Robert D. Finn, José de la Fuente & Margarita Villar To cite this article: José Francisco Lima-Barbero, Sandra Díaz-Sanchez, Olivier Sparagano, Robert D. Finn, José de la Fuente & Margarita Villar (2019) Metaproteomics characterization of the alphaproteobacteria microbiome in different developmental and feeding stages of the poultry red mite Dermanyssusgallinae (De Geer, 1778), Avian Pathology, 48:sup1, S52-S59, DOI: 10.1080/03079457.2019.1635679 To link to this article: https://doi.org/10.1080/03079457.2019.1635679 © 2019 The Author(s). Published by Informa View supplementary material UK Limited, trading as Taylor & Francis Group Accepted author version posted online: 03 Submit your article to this journal Jul 2019. Published online: 02 Aug 2019. Article views: 694 View related articles View Crossmark data Citing articles: 3 View citing articles Full Terms & Conditions of access and use can be found at https://www.tandfonline.com/action/journalInformation?journalCode=cavp20 AVIAN PATHOLOGY 2019, VOL. 48, NO. S1, S52–S59 https://doi.org/10.1080/03079457.2019.1635679 ORIGINAL ARTICLE Metaproteomics characterization of the alphaproteobacteria microbiome in different developmental and feeding stages of the poultry red mite Dermanyssus gallinae (De Geer, 1778) José Francisco Lima-Barbero a,b, Sandra Díaz-Sanchez a, Olivier Sparagano c, Robert D. Finn d, José de la Fuente a,e and Margarita Villar a aSaBio. -
Supplementary Materials A
Supplementary Materials A. Different tests for identification of bacteria Gram’s staining: This test categorizes organisms into two large groups: Gram-positive and Gram- negative. In this test, there are basically four steps: primary staining with crystal violet to a heat-fixed smear, followed by the addition of mordant (Gram’s iodine), rapid decolorization with alcohol or mixture of alcohol and acetone, and finally counterstaining with safranin. Gram-positive bacteria appear as purple, and Gram-negative bacteria appear as pink or red. Spore staining: This staining helps to determine whether the bacterium is a spore producer or not. The staining method uses malachite green as the primary stain and safranin as the counterstain. If spores are present, they appear as bright green, while vegetative cells appear as brownish red to pink. Catalase test: This test determines whether the bacterium contains a catalase enzyme or not. For this, the bacterium inoculum is taken with the non-iron loop and smeared on the surface of the glass slide. Then, 2-4 drops of hydrogen peroxide are added to the smear. If bubbles are observed, the bacterium is catalase-positive; otherwise, it is regarded as catalase-negative. Oxidase test: This test determines the presence of cytochrome oxidase enzyme. For this, an inoculum of pure bacterial culture is added to the surface of the test strip, impregnated with the reagent. Purple color observed within 5-10 seconds indicates that the bacterium has the oxidase enzyme. Methyl red–Voges Proskauer (MR-VP) test: This consists of two tests. 1. The MR test is useful for determining the fermentation pathway for glucose utilization.