Multicenter Evaluation Supports Accuracy of the Beckman Coulter
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Redalyc.Establishment of a Pathogenicity Index for One-Day-Old
Revista Brasileira de Ciência Avícola ISSN: 1516-635X [email protected] Fundação APINCO de Ciência e Tecnologia Avícolas Brasil Pilatti, RM; Furian, TQ; Lima, DA; Finkler, F; Brito, BG; Salle, CTP; Moraes, HLS Establishment of a Pathogenicity Index for One-day-old Broilers to Pasteurella multocida Strains Isolated from Clinical Cases in Poultry and Swine Revista Brasileira de Ciência Avícola, vol. 18, núm. 2, abril-junio, 2016, pp. 255-260 Fundação APINCO de Ciência e Tecnologia Avícolas Campinas, Brasil Available in: http://www.redalyc.org/articulo.oa?id=179746750008 How to cite Complete issue Scientific Information System More information about this article Network of Scientific Journals from Latin America, the Caribbean, Spain and Portugal Journal's homepage in redalyc.org Non-profit academic project, developed under the open access initiative Brazilian Journal of Poultry Science Revista Brasileira de Ciência Avícola Establishment of a Pathogenicity Index for One-day- ISSN 1516-635X May - Jun 2016 / v.18 / n.2 / 255-260 old Broilers to Pasteurella multocida Strains Isolated http://dx.doi.org/10.1590/1806-9061-2015-0089 from Clinical Cases in Poultry and Swine Author(s) ABSTracT Pilatti RMI Although Pasteurella multocida is a member of the respiratory Furian TQI microbiota, under some circumstances, it is a primary agent of diseases Lima DAI , such as fowl cholera (FC), that cause significant economic losses. Finkler FII Brito BGII Experimental inoculations can be employed to evaluate the pathogenicity Salle CTPI of strains, but the results are usually subjective and knowledge on the Moraes HLSI pathogenesis of this agent is still limited. -
ﺑﺴﻢ اﷲ اﻟﺮﺣﻤﻦ اﻟﺮﺣﻴﻢ Molecular Characterization Of
View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by KhartoumSpace ﺑﺴﻢ اﷲ اﻟﺮﺣﻤﻦ اﻟﺮﺣﻴﻢ Molecular Characterization of Pasteurella multocida Vaccine Strains By: Hajir Badawi Mohammed Ahmed B.V.M Khartoum University (2006) Supervisor: Dr. Awad A. Ibrahim A dissertation submitted to the University of Khartoum in partial fulfillment of the requirements for the degree of M. Sc. in Microbiology Department of Microbiology, Faculty of Veterinary Medicine, University of Khartoum June, 2010 Dedication To my mother Father Brother, sister and friends With great love Acknowledgments First and foremost, I would like to thank my Merciful Allah, the most beneficent for giving me strength and health to accomplish this work. Then I would like to deeply thank my supervisor Dr. Awad A. Ibrahim for his advice, continuous encouragement and patience throughout the period of this work. My gratitude is also extended to prof. Mawia M. Mukhtar and for Dr. Manal Gamal El-dein, Institute of Endemic Disease. My thanks extend to members of Department of Microbiology Faculty of Veterinary Medicine for unlimited assistant and for staff of Central Laboratory Soba. I am grateful to my family for their continuous support and standing beside me all times. My thanks also extended to all whom I didn’t mention by name and to the forbearance of my friends, and colleagues who helped me. Finally I am indebted to all those who helped me so much to make this work a success. Abstract The present study was carried out to study the national haemorrhagic septicaemia vaccine strains at their molecular level. -
Pasteurella Multocida Isolated from Cattle
Journal of Applied Pharmaceutical Science Vol. 3 (04), pp. 106-110, April, 2013 Available online at http://www.japsonline.com DOI: 10.7324/JAPS.2013.3419 ISSN 2231-3354 Antibiotic Susceptibility and Molecular Analysis of Bacterial Pathogen Pasteurella Multocida Isolated from Cattle Azmat Jabeen, Mahrukh Khattak, Shahzad Munir*, Qaiser Jamal, Mubashir Hussain Department of Microbiology, Kohat University of Science and Technology, Kohat, Khyber Pakhtunkhwa, Pakistan. ARTICLE INFO ABSTRACT Article history: Pasteurella multocida is a Gram negative, non motile and coccobacillus bacterium. It has 5 strains i.e. A, B, D, Received on: 01/02/2013 E and F and 16 serotypes (1-16). In present study, we analyzed Pasteurella multocida B: 2 strains, responsible Revised on: 19/02/2013 for Hemorrhagic Septicemia (HS) in cattle, on morphological/microbial, biochemical, molecular level and to Accepted on: 15/03/2013 check the antibiotic sensitivity of the Pasteurella multocida. Microbial analysis showed that while grown on Available online: 27/04/2013 Brain Heart Infusion agar plates and Blood Agar Base Medium, grayish lustrous colonies of Pasteurella multocida were observed. Gram staining showed that Pasteurella multocida are gram negative. Microscopic Key words: observations revealed it to be coccobacillus and it was non- motile. Identification was conducted by Pasteurella multocida, conventional biochemical tests and percentage identification of Analytical Profile Index was 96 %. Antibiotic Hemorrhagic Septicemia, sensitivity with different antibiotics was checked by disk diffusion method and was found resistant to Analytical Profile Index, Augmentin, Amoxicillin and Aztreonam and was more susceptible to Ceftiofur. On molecular level its DNA Antibiotic sensitivity. was extracted and was run with marker having range from 0.5 – 10 kb. -
Delineation of Aeromonas Hydrophila Pathotypes by Dectection of Putative Virulence Factors Using Polymerase Chain Reaction and N
View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by DigitalCommons@Kennesaw State University Kennesaw State University DigitalCommons@Kennesaw State University Master of Science in Integrative Biology Theses Biology & Physics Summer 7-20-2015 Delineation of Aeromonas hydrophila Pathotypes by Dectection of Putative Virulence Factors using Polymerase Chain Reaction and Nematode Challenge Assay John Metz Kennesaw State University, [email protected] Follow this and additional works at: http://digitalcommons.kennesaw.edu/integrbiol_etd Part of the Integrative Biology Commons Recommended Citation Metz, John, "Delineation of Aeromonas hydrophila Pathotypes by Dectection of Putative Virulence Factors using Polymerase Chain Reaction and Nematode Challenge Assay" (2015). Master of Science in Integrative Biology Theses. Paper 7. This Thesis is brought to you for free and open access by the Biology & Physics at DigitalCommons@Kennesaw State University. It has been accepted for inclusion in Master of Science in Integrative Biology Theses by an authorized administrator of DigitalCommons@Kennesaw State University. For more information, please contact [email protected]. Delineation of Aeromonas hydrophila Pathotypes by Detection of Putative Virulence Factors using Polymerase Chain Reaction and Nematode Challenge Assay John Michael Metz Submitted in partial fulfillment of the requirements for the Master of Science Degree in Integrative Biology Thesis Advisor: Donald J. McGarey, Ph.D Department of Molecular and Cellular Biology Kennesaw State University ABSTRACT Aeromonas hydrophila is a Gram-negative, bacterial pathogen of humans and other vertebrates. Human diseases caused by A. hydrophila range from mild gastroenteritis to soft tissue infections including cellulitis and acute necrotizing fasciitis. When seen in fish it causes dermal ulcers and fatal septicemia, which are detrimental to aquaculture stocks and has major economic impact to the industry. -
Experimental Infection of Aeromonas Hydrophila in Pangasius J Sarker1, MAR Faruk*
Progressive Agriculture 27 (3): 392-399, 2016 ISSN: 1017 - 8139 Experimental infection of Aeromonas hydrophila in pangasius J Sarker1, MAR Faruk* Department of Aquaculture, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh, 1Department of Aquaculture, Chittagong Veterinary and Animal Sciences University, Khulshi, Chittagong, Bangladesh Abstract Experimental infections of Aeromonas hydrophila in juvenile pangasius (Pangasianodon hypophthalmus) were studied. Five different challenge routes included intraperitoneal (IP) injection, intramuscular (IM) injection, oral administration, bath and agar implantation were used with different preparations of the bacteria to infect fish. The challenge experiments were continued for 15 days. A challenge dose of 4.6×106 colony forming unit (cfu) fish-1 was used for IP and IM injection and oral administration method. Generally, IP route was found more effective for infecting and reproducing clinical signs in fish that caused 100% mortality at the end of challenge. IM injection, oral and bath administration routes were also found effective for infecting and reproducing the clinical signs in fish to some extent. Agar implantation with fresh colonies of bacteria also caused 100% mortality of challenged fish very quickly with no visible clinical signs in fish. The major clinical signs of challenged fish included reddening around eyes and mouth, bilateral exophthalmia, hemorrhage and ulceration at fin bases and fin erosion. Key words: Experimental infection, Aeromonas hydrophila, pangasius Progressive Agriculturists. All rights reserve *Corresponding Author: [email protected] Introduction Bacterial diseases are the most common infectious fishes in different locations of Bangladesh (Rahman problem of commercial fish farms causing much and Chowdhury, 1996; Sarker et al., 2000; Alam, mortality. Among the bacterial genera, Aeromonas spp. -
Aeromonas Veronii Biovar Sobria Gastoenteritis: a Case Report
iMedPub Journals 2011 ARCHIVES OF CLINICAL MICROBIOLOGY Vol. 2 No. 5:3 This article is available from: http://www.acmicrob.com doi: 10:3823/240 Aeromonas veronii biovar sobria gastoenteritis: a case report Afreenish Hassan*, Javaid Usman, Fatima Kaleem, National University of Sciences and Technology, Islamabad, Pakistan Maria Omair, Ali Khalid, Muhammad Iqbal * Corresponding author: Dr Afreenish Hassan Abstract E-mail: [email protected] Aeromonas veronii biovar sobria is associated with various infections in humans. Isola- tion of Aeromonas sobria in patients with gastroenteritis is not unusual. We describe a case of Aeromonas veronii biovar sobria gastroenteritis in a young patient. This is the first documented case reported from Pakistan. Introduction were collected for laboratory investigation. He was shifted to the medical ward and was started on Inj. Ciprofloxacin 200mg The genus Aeromonas include many species but the most twice daily, infusion Metronidazole 500mg three times a day, common ones associated with human infections are Aeromo- injection Maxolon 10 mg three times a day. He was rehydrated nas veronii, Aeromons hydrophila, Aeromonas jandaei, Aeromo- with infusion Normal saline 1000ml once daily. He was advised nas caviae and Aeromonas schubertii [1]. The diseases caused to take orally Oral Rehydration salt (ORS). His blood complete by Aeromonas include gastroenteritis, ear and wound infec- picture and urine routine examination was unremarkable ex- tions, cellulitis, urinary tract infections and septicemia [2]. We cept mildly raised neutrophil count in blood (73%) (Table 1,2,3). describe here a case of Aeromonas veronii biovar sobria gastro- On gross examination, his stool sample was of green in colour, enteritis in a young patient. -
Clinical Antibiotic Guidelines†
CLINICAL ANTIBIOTIC GUIDELINES† ACYCLOVIR IV*/PO *RESTRICTED TO ANTIBIOTIC FORM Predictable activity: Unpredictable activity: No activity: Herpes Simplex Cytomegalovirus Epstein Barr Virus Herpes Zoster Indicated: IV: 1. Therapy for suspected or documented Herpes simplex encephalitis 2. Therapy for suspected or documented Herpes simplex infection of a newborn or immunocompromised patient 3. Therapy for primary varicella infection in immunocompromised patients 4. Therapy for severe or disseminated varicella-zoster infections in immunocompromised or immunocompetent patient 5. Therapy for primary genital herpes with neurologic complications Oral: 1. Therapy for primary Herpes simplex infections (oral/genital) 2. Suppressive (preventative) therapy for recurrent (³ 6 episodes/year) severe Herpes simplex infections (oral/genital) 3. Episodic therapy for recurrent (³ 6 episodes/year) Herpes simplex genital infections (initiate within 24 hours of prodrome onset) 4. Prophylaxis for HSV in bone marrow transplants where patient is seropositive 5. Therapy and suppressive therapy for Eczema Herpeticum 6. Therapy for varicella-zoster infections in immunocompetent and immunocompromised patients (if not severe) 7. Therapy for primary varicella infections in pregnancy 8. Therapy for varicella in immunocompetent patients > 13 years old (initiate within 24 hours of rash onset) 9. Therapy for varicella in patients < 13 years old (initiate within 24 hours of rash onset) if there is a chronic cutaneous or pulmonary disorder, long term salicylate therapy, or short, intermittent or aerosolized corticosteroid use Not Indicated: 1. Therapy for acute Epstein-Barr infections (acute mononucleosis) 2. Therapy for documented CMV infections CLINICAL ANTIBIOTIC GUIDELINES† AMIKACIN RESTRICTED TO ANTIBIOTIC FORM Predictable activity: Unpredictable activity: No activity: Enterobacteriaceae Staphylococcus spp Streptococcus spp Pseudomonas spp Enterococcus spp some Mycobacterium spp Alcaligenes spp Anaerobes Indicated: 1. -
An Update on the Genus Aeromonas: Taxonomy, Epidemiology, and Pathogenicity
microorganisms Review An Update on the Genus Aeromonas: Taxonomy, Epidemiology, and Pathogenicity Ana Fernández-Bravo and Maria José Figueras * Unit of Microbiology, Department of Basic Health Sciences, Faculty of Medicine and Health Sciences, IISPV, University Rovira i Virgili, 43201 Reus, Spain; [email protected] * Correspondence: mariajose.fi[email protected]; Tel.: +34-97-775-9321; Fax: +34-97-775-9322 Received: 31 October 2019; Accepted: 14 January 2020; Published: 17 January 2020 Abstract: The genus Aeromonas belongs to the Aeromonadaceae family and comprises a group of Gram-negative bacteria widely distributed in aquatic environments, with some species able to cause disease in humans, fish, and other aquatic animals. However, bacteria of this genus are isolated from many other habitats, environments, and food products. The taxonomy of this genus is complex when phenotypic identification methods are used because such methods might not correctly identify all the species. On the other hand, molecular methods have proven very reliable, such as using the sequences of concatenated housekeeping genes like gyrB and rpoD or comparing the genomes with the type strains using a genomic index, such as the average nucleotide identity (ANI) or in silico DNA–DNA hybridization (isDDH). So far, 36 species have been described in the genus Aeromonas of which at least 19 are considered emerging pathogens to humans, causing a broad spectrum of infections. Having said that, when classifying 1852 strains that have been reported in various recent clinical cases, 95.4% were identified as only four species: Aeromonas caviae (37.26%), Aeromonas dhakensis (23.49%), Aeromonas veronii (21.54%), and Aeromonas hydrophila (13.07%). -
Interaction Study of Pasteurella Multocida with Culturable Aerobic
Hanchanachai et al. BMC Microbiology (2021) 21:19 https://doi.org/10.1186/s12866-020-02071-4 RESEARCH ARTICLE Open Access Interaction study of Pasteurella multocida with culturable aerobic bacteria isolated from porcine respiratory tracts using coculture in conditioned media Nonzee Hanchanachai1,2, Pramote Chumnanpuen2,3 and Teerasak E-kobon2,4,5* Abstract Background: The porcine respiratory tract harbours multiple microorganisms, and the interactions between these organisms could be associated with animal health status. Pasteurella multocida is a culturable facultative anaerobic bacterium isolated from healthy and diseased porcine respiratory tracts. The interaction between P. multocida and other aerobic commensal bacteria in the porcine respiratory tract is not well understood. This study aimed to determine the interactions between porcine P. multocida capsular serotype A and D strains and other culturable aerobic bacteria isolated from porcine respiratory tracts using a coculture assay in conditioned media followed by calculation of the growth rates and interaction parameters. Results: One hundred and sixteen bacterial samples were isolated from five porcine respiratory tracts, and 93 isolates were identified and phylogenetically classified into fourteen genera based on 16S rRNA sequences. Thirteen isolates from Gram-negative bacterial genera and two isolates from the Gram-positive bacterial genus were selected for coculture with P. multocida. From 17 × 17 (289) interaction pairs, the majority of 220 pairs had negative interactions indicating competition for nutrients and space, while 17 pairs were identified as mild cooperative or positive interactions indicating their coexistence. All conditioned media, except those of Acinetobacter, could inhibit P. multocida growth. Conversely, the conditioned media of P. multocida also inhibited the growth of nine isolates plus themselves. -
Comparative Pathogenomics of Aeromonas Veronii from Pigs in South Africa: Dominance of the Novel ST657 Clone
microorganisms Article Comparative Pathogenomics of Aeromonas veronii from Pigs in South Africa: Dominance of the Novel ST657 Clone Yogandree Ramsamy 1,2,3,* , Koleka P. Mlisana 2, Daniel G. Amoako 3 , Akebe Luther King Abia 3 , Mushal Allam 4 , Arshad Ismail 4 , Ravesh Singh 1,2 and Sabiha Y. Essack 3 1 Medical Microbiology, College of Health Sciences, University of KwaZulu-Natal, Durban 4000, South Africa; [email protected] 2 National Health Laboratory Service, Durban 4001, South Africa; [email protected] 3 Antimicrobial Research Unit, College of Health Sciences, University of KwaZulu-Natal, Durban 4000, South Africa; [email protected] (D.G.A.); [email protected] (A.L.K.A.); [email protected] (S.Y.E.) 4 Sequencing Core Facility, National Institute for Communicable Diseases, National Health Laboratory Service, Johannesburg 2131, South Africa; [email protected] (M.A.); [email protected] (A.I.) * Correspondence: [email protected] Received: 9 November 2020; Accepted: 15 December 2020; Published: 16 December 2020 Abstract: The pathogenomics of carbapenem-resistant Aeromonas veronii (A. veronii) isolates recovered from pigs in KwaZulu-Natal, South Africa, was explored by whole genome sequencing on the Illumina MiSeq platform. Genomic functional annotation revealed a vast array of similar central networks (metabolic, cellular, and biochemical). The pan-genome analysis showed that the isolates formed a total of 4349 orthologous gene clusters, 4296 of which were shared; no unique clusters were observed. All the isolates had similar resistance phenotypes, which corroborated their chromosomally mediated resistome (blaCPHA3 and blaOXA-12) and belonged to a novel sequence type, ST657 (a satellite clone). -
Contagious Antibiotic Resistance: Plasmid Transfer Among Bacterial Residents Of
bioRxiv preprint doi: https://doi.org/10.1101/2020.11.09.375964; this version posted November 10, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 1 Contagious Antibiotic Resistance: Plasmid Transfer Among Bacterial Residents of 2 the Zebrafish Gut. 3 4 Wesley Loftie-Eaton1,2, Angela Crabtree1, David Perry1, Jack Millstein1,2, Barrie 5 Robinson1,2, Larry Forney1,2 and Eva Top1,2 # 6 7 1Department of Biological Sciences, 2Institute for Bioinformatics and Evolutionary Studies 8 (IBEST), University of Idaho, PO Box 443051, Moscow, Idaho, USA. Phone: +1-208-885- 9 8858; E-mail: [email protected]; Fax: 208-885-7905 10 # Corresponding author: Department of Biological Sciences, Institute for Bioinformatics 11 and Evolutionary Studies (IBEST), University of Idaho, PO Box 443051, Moscow, Idaho, 12 USA. Phone: +1-208-885-5015; Email: [email protected]; Fax: 208-885-7905 13 14 15 Running title: Plasmid transfer in the zebrafish gut microbiome 16 1 bioRxiv preprint doi: https://doi.org/10.1101/2020.11.09.375964; this version posted November 10, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 17 Abstract 18 By characterizing the trajectories of antibiotic resistance gene transfer in bacterial 19 communities such as the gut microbiome, we will better understand the factors that 20 influence this spread of resistance. -
Metaproteomics Characterization of the Alphaproteobacteria
Avian Pathology ISSN: 0307-9457 (Print) 1465-3338 (Online) Journal homepage: https://www.tandfonline.com/loi/cavp20 Metaproteomics characterization of the alphaproteobacteria microbiome in different developmental and feeding stages of the poultry red mite Dermanyssus gallinae (De Geer, 1778) José Francisco Lima-Barbero, Sandra Díaz-Sanchez, Olivier Sparagano, Robert D. Finn, José de la Fuente & Margarita Villar To cite this article: José Francisco Lima-Barbero, Sandra Díaz-Sanchez, Olivier Sparagano, Robert D. Finn, José de la Fuente & Margarita Villar (2019) Metaproteomics characterization of the alphaproteobacteria microbiome in different developmental and feeding stages of the poultry red mite Dermanyssusgallinae (De Geer, 1778), Avian Pathology, 48:sup1, S52-S59, DOI: 10.1080/03079457.2019.1635679 To link to this article: https://doi.org/10.1080/03079457.2019.1635679 © 2019 The Author(s). Published by Informa View supplementary material UK Limited, trading as Taylor & Francis Group Accepted author version posted online: 03 Submit your article to this journal Jul 2019. Published online: 02 Aug 2019. Article views: 694 View related articles View Crossmark data Citing articles: 3 View citing articles Full Terms & Conditions of access and use can be found at https://www.tandfonline.com/action/journalInformation?journalCode=cavp20 AVIAN PATHOLOGY 2019, VOL. 48, NO. S1, S52–S59 https://doi.org/10.1080/03079457.2019.1635679 ORIGINAL ARTICLE Metaproteomics characterization of the alphaproteobacteria microbiome in different developmental and feeding stages of the poultry red mite Dermanyssus gallinae (De Geer, 1778) José Francisco Lima-Barbero a,b, Sandra Díaz-Sanchez a, Olivier Sparagano c, Robert D. Finn d, José de la Fuente a,e and Margarita Villar a aSaBio.