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Microbiology Standard Operating Procedures (Sops) Microbiology Standard Operating Procedures (SOPs) وزارة الصحة - اﻹدارة العامة للمستشفٍات - دائرة مختبرات وبنوك دم المستشفٍات Microbiology Department STANDARD OPERATING PROCEDURES (SOPs) April 2016 1 Microbiology Standard Operating Procedures (SOPs) وزارة الصحة - اﻹدارة العامة للمستشفٍات - دائرة مختبرات وبنوك دم المستشفٍات اهداء: اىل أرواح شهدائنا اﻷبزار واىل أرواح شهدائنا خاصة يف يهنة انتحانٍم انطبٍة انشهٍد/ رايً انسهىت و انشهٍد/ حساو راضً وكذنك اىل روح انشيٍم/ حساو أبى مشانة و روح انشيٍم/ ادٌب عهىاٌ واىل يزضاَا انشيﻻء/ يهند انشنطً وانشيٍم حمًىد اجلزو )وهى عضى فزٌق اعداد SOPs املٍكزوبٍىنىجً( َسال اهلل هلى انشفاء انعاجم ونهشهداء انفزدوص اﻷعهى. اىل كم يٍ ٌنتًً اىل هذه املهنةاﻻَساٍَة يٍ سيٍﻻت وسيﻻء هندي هذا انعًم ريشاً نهىفاء 2 Microbiology Standard Operating Procedures (SOPs) وزارة الصحة - اﻹدارة العامة للمستشفٍات - دائرة مختبرات وبنوك دم المستشفٍات شكز وتقدٌز يتقـــــــ م فريـــــق عمـــخ برتتـــروك َمىـــُت فم و مجتيـــلشكك ماليـــخ و يـــ ر َو تقـــ ير إ ــــ كـــخ بــــه اﻷخ الدددور/ يو ف ادددا ايددد الدددافة الرحددد ل اي ال ددد ا، َإ ـــ اﻷخ الدددور/ يو دددو اللطحدددا ال ددد ما ردددوفا ددد ي ال / ددد ح " مـــك وـــ بُي بـــه فعـــ َلجـــٍشخ بـــه و ـــخ وواـــك ٌــــلو و ومــــخ َكــــل إ ــــ اﻻادددد/ او دددد را ايدددد دددد تا رددددوفا دا ددددا ر / دددداا لي دددد دي ال / ح ا يق ا مك و بً به فع َبيُرة في مشع بروحخ وواك ٌلو و ومخ . كمـــك وتقـــ م ماليـــخ و يـــ ر َو تقـــ ير و ـــ كـــخ بـــه فريـــق واعـــ وف َفريـــق و مرو وـــ مـــك مـــل ُي بــه ٍــ بــه و ــخ ولمــكم ٌــلو و ومــخ َكــل و ــ مشــع وارــُة و وــكب شه فــي برتتــروك َمىــُت فم و مجتيلشكك و ليه سش ُن ٍ و َر و روئ في لطتشق ٌلي وا روءوك. وكذذذ لك نت ذذذدم بلزٍذذذا الشذذذكر والعرةذذذاو الذذذي ملسسذذذة العذذذوو ال بذذذٌ للفلسذذذ ٍنٍٍو لر ذذذاٍت م با ة ه ا الدلٍا. 3 Microbiology Standard Operating Procedures (SOPs) وزارة الصحة - اﻹدارة العامة للمستشفٍات - دائرة مختبرات وبنوك دم المستشفٍات بسم هللا الرحمو الرحٍم قاا تعالي: ) و قا ا ملوا ةسٍرى هللا ملكم ورسوله و الملمنوو( صدق هللا العظٍم اﻷخوة واﻷخوات الزمﻻء اﻷكارم... السﻻم لٍكم ورحمة هللا وبركاته..... أما بعد انذذه لمذذو دوا ذذٌ سذذرورً أو أيذذك بذذٍو أٍذذدٍكم ) دلٍذذا ذذرق الفحوصذذات المخبرٍذذة الموحذذد( (Standard Operating Procedure SOPs) والذذ ً ٍتيذذمو توًٍ ذذا وتفصٍﻻ ل رق ما الفحوصات التٌ ت دم ا مختبرات وبنوك دم المستشفٍات, و لك مذو ألذا تنظٍم العما ويماو لودته ةٌ لمٍك لوانب خدمات المختبرات وبنوك الدم. تأتٌ الحالة إلي ه ا العمذا تأكٍذدا وترسذٍخا لمبذدأ العمذا بذرول الفرٍذق الواحذد وتوًٍذق وتوحٍذد رق العما المبنٌ لي أسس لمٍة وال ً تنت لذه وزارة الصذحة واﻹدارة العامذة للمستشذفٍات ةٌ كاةة مناحٌ العمذا وت لعذا للرقذٌ بم نذة التحالٍذا ال بٍذة خاصذ ة, و بلذودة الخذدمات ال بٍذة الم دمة ةٌ الوزارة بشكا ام. كما ٍعتبر ه ا الدلٍا اﻷوا والموحد للمٍك مختبرات وبنذوك دم المستشذفٍات و الذ ً سذٍكوو لذه بمشٍئة هللا اﻷًر اﻻٍلابٌ لي لودة الخدمات المخبرٍة وملمذا اﻷداء الفنذٌ مذو ألذا اﻻرت ذاء بالخدمات الم دمة للموا و. ةٌ الن اٍة ﻻ ٍسعنٌ إﻻ أو أت دم بخالص الشكر و الت دٍر لفرٍق ما دائرة مختبرات وبنوك دم المستشفٍات ولمٍك مو ساهم ةذٌ انلذاز هذ ا العمذا سذوا ء ةذٌ اﻹ ذداد أو المرالعذة أو ال با ذة واﻹخراج. د. عبد اللطيف الحاج روفا ي ال / ح 4 Microbiology Standard Operating Procedures (SOPs) وزارة الصحة - اﻹدارة العامة للمستشفٍات - دائرة مختبرات وبنوك دم المستشفٍات فافق اﻻ واد اﻻسم مكان العمل ناهض زات بد الل ٍف ملمك الشفاء ال بٌ محمود بد الحكٍم اللرو ملمك الشفاء ال بٌ اسامه زكرٍا اللوراني مستشفي غزة اﻷوروبٌ ةلاير شعباو وض مستشفي النصر لﻷ فاا مرٍاو سلٍم الخيرى مستشفي د. بد العزٍز الرنتٍسٌ التخصصٌ محمد دناو اللدى مستشفي الش ٍد محمد الدرة ا مر النلار مستشفي الش ٍد أبو ٍوسف النلار فافق ال ااج اﻻام رك ت ال عمش بيتٍ وﻹفورة و وكب مجتيلشكك رش ي رصرص بجتيل غلة واَرَمي مكل و يشخ فيب بجتيل و ىصر ﻷطلكل ككي أحم بامع و يلكء و طتي سحر غكو وﻹفورة و وكب مجتيلشكك 5 Microbiology Standard Operating Procedures (SOPs) وزارة الصحة - اﻹدارة العامة للمستشفٍات - دائرة مختبرات وبنوك دم المستشفٍات Contents SOPs S No Subject NO. Quality Assurance In Microbiology (Equipment, Safety Measures SPs 01 1 Blood Agar Preparation and Sterilization SPs 02 2 Chocolate Agar Preparation and Sterilization SPs 03 3 MacConky Agar Preparation and Sterilization SPs 04 4 Muller Hinton Agar Preparation and Sterilization SPs 05 5 Sabouraud Dextrose Agar Preparation and Sterilization SPs 06 6 Shigella Salmonella Aagr Preparation and Sterilization SPs 07 7 Hekton Enteric Aagr Preparation and Sterilization SPs 08 8 Xylose lysine deoxycholate Preparation and Sterilization SPs 09 9 Fluid Selenite Cystine Medium Preparation and Sterilization SPs 10 10 Catalase Test SPs 11 11 Bacitracin test SPs 12 12 Optochin susceptibility test SPs 13 13 Oxidase test (Kovac's Method) SPs 14 14 Coagulase tube test SPs 15 15 Slide Staphylococcus aureus Kit SPs 16 16 6 Microbiology Standard Operating Procedures (SOPs) وزارة الصحة - اﻹدارة العامة للمستشفٍات - دائرة مختبرات وبنوك دم المستشفٍات DNase test SPs 17 17 Novobiocin test SPs 18 18 Bile Esculin Agar Slant SPs 19 19 Streptococcus – Check Kit SPs 20 20 SPs 21 21 API 20 E test SPs 22 22 Gram stain SPs 23 23 Acid fast stain SPs 24 24 KOH test SPs 25 25 Meningitides Antisera SPs 26 26 Cerebrospinal fluid (CSF) SPs 27 27 Body Fluid Culture, Sterile SPs 28 28 Blood Culture SPs 29 29 Throat Swab Culture SPs 30 30 Sputum Culture SPs 31 31 Pus (wound, Abscesses, Burns and sinuses) culture SPs 32 32 Genital Culture SPs 33 33 Urine culture SPs 34 34 Stool Culture SPs 35 35 Antibiotic Susceptibility Test In Clinical Laboratory SPs 36 36 INDEX 7 Microbiology Standard Operating Procedures (SOPs) وزارة الصحة - اﻹدارة العامة للمستشفٍات - دائرة مختبرات وبنوك دم المستشفٍات Quality Assurance In Microbiology (Equipment ,Safety Measures) SOPs\HGA \......H\ M / 01 Version: …1……………………………....... Head of department:…..…………………….. Date effective: ..……………………....... Quality Officer: ………………………............ Copy number: ..………...................... Director of :……………………………............ Introduction Microbiology can be defined as a branch of biology which is studying of microorganisms that cant seen by naked eye (bacteria-viruses-fungi-parasite). Quality assurance programmers are an efficient way of maintaining the standards of performance of diagnostic laboratories, and of upgrading those standards where necessary. In microbiology, quality goes beyond technical perfection to take into account the speed, cost, and usefulness or clinical relevance of the test. Purpose & Definition: To be of good quality, a diagnostic test must be clinically relevant, i.e. it must help in the prevention or treatment of disease. Care of equipment: It is particularly important to take good care of laboratory equipment. Good quality tests cannot be performed if the equipment used is either of poor quality or poorly maintained. Maintenance Of Equipment : Good quality equipment is absolutely essential to generate quality results . Suggested maintenance of commonly used equipment : Autoclave : 1. Adjust Clean and change water monthly . 2. Adjust water level before each run . 3. Record time , temperature and pressure for each run . 4. Inspect gasket in the lid weekly . 5. Technical inspection every six months . Incubator: 1. Clean inside walls once in a month . 8 Microbiology Standard Operating Procedures (SOPs) وزارة الصحة - اﻹدارة العامة للمستشفٍات - دائرة مختبرات وبنوك دم المستشفٍات 2. Record temperature at the start of each working day . 3. Technical maintenance every six months . Microscope : 1. Wipe lenses with lens paper at the end of each day’s work . 2. Protect the microscope from dust , vibrations , and moisture . 3. Place a shallow plate containing dry blue silica gel in a box to absorb moisture 4. Check alignment of the condenser once a month . 5. Technical inspection once in a year . Balance : 1. Keep the balance clean and dry . 2. Always use a container or weighing paper , do not put material directly on the pan Prevent the balance from drafts of air . Refrigerator: 1. Place at least 10 inches away from the wall . 2. Clean and defrost at least every two months . 3. Records temperature daily . 4. Technical service at least once a year . Centrifuge : 1. Wipe inner walls with antiseptic solution weekly . 2. Check brushes and bearings every six months . Laboratory Safety Measures: Every microbiology laboratory must have a strictly enforced safety program to protect the technical personnel working in the area , to ensure the validity of the testing procedures by preventing contamination , and to prevent the spread to infectious microbial agents . The following regulations are recommended for the safety program of general microbiology laboratories: 1. Benches should be washed with a suitable disinfectant (e.g. chlorine compound) before work is begun. 3. When work is completed , all benches should be disinfected again with a suitable disinfectant . 4. Infectious organisms should be performed in safety cabinet. 9 Microbiology Standard Operating Procedures (SOPs) وزارة الصحة - اﻹدارة العامة للمستشفٍات - دائرة مختبرات وبنوك دم المستشفٍات 5. Infectious Materials should be placed in biohazard containers and sent to incineration. 6. The laboratory is restricted access to authorized personnel only . 7. No smoking , drinking , or eating is permitted in the area . 8. No mouth pipetting is allowed in the lab . 9. All syringes should be placed in a puncture –proof container . 13. safety cabinet should be checked yearly for efficiency . 14. All biohazard materials should label clearly . 15. The use of gloves is recommended for handling infectious materials . 16. Hands should be washed before and after the work. 17. All spills and accidents should be reported to supervisor. 11 Microbiology Standard Operating Procedures (SOPs) وزارة الصحة - اﻹدارة العامة للمستشفٍات - دائرة مختبرات وبنوك دم المستشفٍات Blood Agar Preparation and Sterilization SOPs \ HGA \......H\ M \ 02 Version: …1…………………………….. Head of department:…..………………….. Date effective: ..………………….. Quality Officer: ……………………….......... Copy number: ..……….............. Director of :…………………………….......... Purpose & Definition: A culture media is special medium to grow different kinds of microorganisms and distinguish the growth characteristics of microorganisms in various differential, selective and enriched media, blood agar used to differentiating hemolytic organisms.
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