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Supplementary Table 1 List of 335 Genes Differentially Expressed Between Primary (P) and Metastatic (M) Tumours

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Supplementary Table 1 List of 335 genes differentially expressed between primary (P) and metastatic (M) tumours Spot ID I.M.A.G.E. UniGene Symbol Name Clone ID Cluster 296529 296529 In multiple clusters 731356 731356 Hs.140452 M6PRBP1 mannose-6-phosphate receptor binding protein 1 840942 840942 Hs.368409 HLA-DPB1 major histocompatibility complex, class II, DP beta 1 142122 142122 Hs.115912 AFAP actin filament associated protein 1891918 1891918 Hs.90073 CSE1L CSE1 chromosome segregation 1-like (yeast) 1323432 1323432 Hs.303154 IDS iduronate 2-sulfatase (Hunter syndrome) 788566 788566 Hs.80296 PCP4 Purkinje cell protein 4 591281 591281 Hs.80680 MVP major vault protein 815530 815530 Hs.172813 ARHGEF7 Rho guanine nucleotide exchange factor (GEF) 7 825312 825312 Hs.246310 ATP5J ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F6 784830 784830 Hs.412842 C10orf7 chromosome 10 open reading frame 7 840878 840878 Hs.75616 DHCR24 24-dehydrocholesterol reductase 669443 669443 Hs.158195 HSF2 heat shock transcription factor 2 2485436 2485436 Data not found 82903 82903 Hs.370937 TAPBP TAP binding protein (tapasin) 771258 771258 Hs.85258 CD8A CD8 antigen, alpha polypeptide (p32) 85128 85128 Hs.8986 C1QB complement component 1, q subcomponent, beta polypeptide 41929 41929 Hs.39252 PICALM phosphatidylinositol binding clathrin assembly protein 148469 148469 Hs.9963 TYROBP TYRO protein tyrosine kinase binding protein 415145 415145 Hs.1376 HSD11B2 hydroxysteroid (11-beta) dehydrogenase 2 810017 810017 Hs.179657 PLAUR plasminogen activator, urokinase receptor 770859 770859 Hs.149846 ITGB5 integrin, beta 5 839682 839682 Hs.458359 UBE2N ubiquitin-conjugating enzyme E2N (UBC13 homolog, yeast) 487373 487373 Hs.80986 ATP5G1 ATP synthase, H+ transporting, mitochondrial F0 complex, subunit c (subunit 9), isoform 1 786607 786607 Hs.95243 TCEAL1 transcription elongation factor A (SII)-like 1 772111 772111 Hs.287460 PDZK3 PDZ domain containing 3 130156 130156 Hs.372446 AKAP10 A kinase (PRKA) anchor protein 10 139009_1 139009 Hs.418138 FN1 fibronectin 1 41406 41406 Hs.348802 BAMBI BMP and activin membrane-bound inhibitor homolog (Xenopus laevis) 823900 823900 Hs.410715 CENTB2 centaurin, beta 2 739183 739183 Data not found 291216 291216 Hs.82794 CETN2 centrin, EF-hand protein, 2 685371 685371 Hs.423348 MEN1 multiple endocrine neoplasia I 878545 878545 Hs.409634 RPL18 ribosomal protein L18 1461664 1461664 Hs.422857 BCHE butyrylcholinesterase 128783 128783 Hs.407477 PEX13 peroxisome biogenesis factor 13 110788 110788 Hs.56729 LSP1 lymphocyte-specific protein 1 413733 413733 Hs.446641 TIMP1 tissue inhibitor of metalloproteinase 1 (erythroid potentiating activity, collagenase inhibitor) 853988 853988 Hs.108104 UBE2L3 ubiquitin-conjugating enzyme E2L 3 825451 825451 Hs.325948 VDP vesicle docking protein p115 809421 809421 Hs.3192 PCBD 6-pyruvoyl-tetrahydropterin synthase/dimerization cofactor of hepatocyte nuclear factor 1 alpha (TCF1) 825726 825726 Hs.111749 PMS1 PMS1 postmeiotic segregation increased 1 (S. cerevisiae) 42627 42627 Hs.21016 COCH coagulation factor C homolog, cochlin (Limulus polyphemus) 487425 487425 Hs.528302 CETN3 centrin, EF-hand protein, 3 (CDC31 homolog, yeast) 488303 488303 Hs.153884 APACD ATP binding protein associated with cell differentiation 810504 810504 Hs.77422 PLP2 proteolipid protein 2 (colonic epithelium- enriched) 283034 283034 Hs.372616 ARL1 ADP-ribosylation factor-like 1 788286 788286 Hs.439530 DYRK4 dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 4 302310 302310 Hs.232058 CDNA FLJ36340 fis, clone THYMU2006468 782800 782800 Hs.355735 UQCRFS1 ubiquinol-cytochrome c reductase, Rieske iron- sulfur polypeptide 1 289143 289143 Hs.421150 ZCWCC3 zinc finger, CW-type with coiled-coil domain 3 149013 149013 Hs.159118 AMD1 adenosylmethionine decarboxylase 1 878129 878129 Hs.157429 SOX3 SRY (sex determining region Y)-box 3 784319 784319 Data not found 839888 839888 Hs.7768 FIBP fibroblast growth factor (acidic) intracellular binding protein 46786 46786 In multiple clusters 783721 783721 Hs.307944 SEPT8 septin 8 796198 796198 Hs.30942 EFNB2 ephrin-B2 204148 204148 Hs.188361 RPS6KA3 ribosomal protein S6 kinase, 90kDa, polypeptide 3 141966 141966 Hs.364941 HSD3B1 hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 809648 809648 Hs.440835 SF1 splicing factor 1 814095 814095 Hs.81118 LTA4H leukotriene A4 hydrolase 179534 179534 Hs.4975 KCNQ2 potassium voltage-gated channel, KQT-like subfamily, member 2 35077 35077 Hs.21639 APEG1 aortic preferentially expressed protein 1 842884_1 842884 Hs.115474 RFC3 replication factor C (activator 1) 3, 38kDa 276449 276449 Hs.255230 GUSB glucuronidase, beta 884766 884766 Hs.35052 TEGT testis enhanced gene transcript (BAX inhibitor 1) 291255 291255 Hs.406455 PSAP prosaposin (variant Gaucher disease and variant metachromatic leukodystrophy) 139009 139009 Hs.418138 FN1 fibronectin 1 34849 34849 In multiple clusters 80399 80399 Hs.68714 SFRS1 splicing factor, arginine/serine-rich 1 (splicing factor 2, alternate splicing factor) 120881 120881 Hs.223025 RAB31 RAB31, member RAS oncogene family 773922 773922 In multiple clusters 714210 714210 Hs.54649 RY1 putative nucleic acid binding protein RY-1 842971 842971 Hs.412597 DSG2 desmoglein 2 842863 842863 Hs.318567 NDRG1 N-myc downstream regulated gene 1 741831 741831 Hs.439312 PLTP phospholipid transfer protein 824728 824728 Hs.25313 MCRS1 microspherule protein 1 782047 782047 Hs.428779 UNQ6077 AAAP6077 1474174 1474174 Hs.367877 MMP2 matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase) 795191 795191 Hs.390623 XPNPEP1 X-prolyl aminopeptidase (aminopeptidase P) 1, soluble 824552 824552 Hs.169388 CD99L2 CD99 antigen-like 2 950367 950367 Hs.7957 ADAR adenosine deaminase, RNA-specific 49352 49352 Hs.386741 ANXA7 annexin A7 771084 771084 Hs.12272 BECN1 beclin 1 (coiled-coil, myosin-like BCL2 interacting protein) 143306 143306 Hs.446566 CDNA clone IMAGE:4801360, with apparent retained intron 2095412 2095412 Hs.449070 RPL13A ribosomal protein L13a 840990 840990 Hs.348387 GSTM4 glutathione S-transferase M4 810117 810117 Hs.75510 ANXA11 annexin A11 1404841 1404841 Data not found 120108 120108 Hs.445534 PER1 period homolog 1 (Drosophila) 35185 35185 Hs.89768 GABRB1 gamma-aminobutyric acid (GABA) A receptor, beta 1 810512 810512 Hs.164226 THBS1 thrombospondin 1 80109_1 80109 In multiple clusters 814381 814381 Hs.75189 DAP death-associated protein 51447 51447 Hs.372679 FCGR3A Fc fragment of IgG, low affinity IIIa, receptor for (CD16) 884355 884355 Data not found 49351 49351 Hs.21432 PLXN3 likely ortholog of mouse plexin 3 868575 868575 Hs.420569 DSIPI delta sleep inducing peptide, immunoreactor 711768 711768 Hs.76057 GALE galactose-4-epimerase, UDP- 703581 703581 Hs.1908 PRG1 proteoglycan 1, secretory granule 47021 47021 Hs.515694 Transcribed sequence with weak similarity to protein sp:O14640 (H.sapiens) DVL1_HUMAN Segment polarity protein dishevelled homolog DVL-1 487704 487704 In multiple clusters 840658 840658 Hs.227429 PTOV1 prostate tumor overexpressed gene 1 232826 232826 Hs.439511 ARPC3 actin related protein 2/3 complex, subunit 3, 21kDa 564846 564846 Hs.1540 THOC1 THO complex 1 814444 814444 Hs.279902 CRSP9 cofactor required for Sp1 transcriptional activation, subunit 9, 33kDa 243181 243181 Hs.110903 CLDN5 claudin 5 (transmembrane protein deleted in velocardiofacial syndrome) 2021468 2021468 Hs.284275 PAK2 p21 (CDKN1A)-activated kinase 2 358643 358643 Hs.23111 FARSLA phenylalanine-tRNA synthetase-like, alpha subunit 826459 826459 Hs.356739 PPP6C protein phosphatase 6, catalytic subunit 488413 488413 Hs.149436 KIF5B kinesin family member 5B 72395 72395 Hs.475654 MSTP9 macrophage stimulating, pseudogene 9 884673 884673 Hs.227823 LOC283820 hypothetical protein LOC283820 289677 289677 Hs.131727 PFAAP5 phosphonoformate immuno-associated protein 5 323474 323474 Hs.286221 ARF1 ADP-ribosylation factor 1 298155 298155 Hs.254593 ACADM acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain 363086 363086 Hs.425633 CKMT1 creatine kinase, mitochondrial 1 (ubiquitous) 811088 811088 Hs.26988 EFNB3 ephrin-B3 2393764 2393764 Hs.443227 RFC5 replication factor C (activator 1) 5, 36.5kDa 123980 123980 Hs.147189 CTDSPL CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase-like 824393 824393 Hs.29285 C9orf60 chromosome 9 open reading frame 60 789011 789011 Hs.83347 AAMP angio-associated, migratory cell protein 760344 760344 Hs.2057 UMPS uridine monophosphate synthetase (orotate phosphoribosyl transferase and orotidine-5'- decarboxylase) 627542 627542 Hs.435726 BLVRA biliverdin reductase A 752873 752873 Data not found 810761 810761 Hs.26126 CLDN10 claudin 10 809784 809784 Hs.79361 KLK6 kallikrein 6 (neurosin, zyme) 591864 591864 Hs.1802 HLA-DOB major histocompatibility complex, class II, DO beta 788832 788832 Hs.296638 GDF15 growth differentiation factor 15 824531 824531 Hs.270 PSCDBP pleckstrin homology, Sec7 and coiled-coil domains, binding protein 83549 83549 Hs.376414 C1R complement component 1, r subcomponent 781222 781222 Hs.354085 TIAF1 TGFB1-induced anti-apoptotic factor 1 43207 43207 Hs.441202 GFRA2 GDNF family receptor alpha 2 768377 768377 In multiple clusters 489626 489626 Hs.75160 PFKM phosphofructokinase, muscle 810104 810104 Hs.355888 PLCB2 phospholipase C, beta 2 366481 366481 Hs.432677 KRT6B keratin 6B 229579 229579 Hs.78979 GLG1 golgi apparatus protein 1 504236 504236 Hs.1905 PRL prolactin 824041 824041 Hs.77608 SFRS9 splicing factor, arginine/serine-rich
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    RESEARCH ARTICLE Heme oxygenase-1 regulates mitochondrial quality control in the heart Travis D. Hull,1,2 Ravindra Boddu,1,3 Lingling Guo,2,3 Cornelia C. Tisher,1,3 Amie M. Traylor,1,3 Bindiya Patel,1,4 Reny Joseph,1,3 Sumanth D. Prabhu,1,4,5 Hagir B. Suliman,6 Claude A. Piantadosi,7 Anupam Agarwal,1,3,5 and James F. George2,3,4 1Department of Medicine, 2Department of Surgery, 3Nephrology Research and Training Center, and 4Comprehensive Cardiovascular Center, University of Alabama at Birmingham, Birmingham, Alabama, USA. 5Department of Veterans Affairs, Birmingham, Alabama, USA. 6Department of Anesthesiology and 7Department of Pulmonary, Allergy and Critical Care, Duke University School of Medicine, Durham, North Carolina, USA. The cardioprotective inducible enzyme heme oxygenase-1 (HO-1) degrades prooxidant heme into equimolar quantities of carbon monoxide, biliverdin, and iron. We hypothesized that HO-1 mediates cardiac protection, at least in part, by regulating mitochondrial quality control. We treated WT and HO-1 transgenic mice with the known mitochondrial toxin, doxorubicin (DOX). Relative to WT mice, mice globally overexpressing human HO-1 were protected from DOX-induced dilated cardiomyopathy, cardiac cytoarchitectural derangement, and infiltration of CD11b+ mononuclear phagocytes. Cardiac-specific overexpression of HO-1 ameliorated DOX-mediated dilation of the sarcoplasmic reticulum as well as mitochondrial disorganization in the form of mitochondrial fragmentation and increased numbers of damaged mitochondria in autophagic vacuoles. HO-1 overexpression promotes mitochondrial biogenesis by upregulating protein expression of NRF1, PGC1α, and TFAM, which was inhibited in WT animals treated with DOX. Concomitantly, HO-1 overexpression inhibited the upregulation of the mitochondrial fission mediator Fis1 and resulted in increased expression of the fusion mediators, Mfn1 and Mfn2.
  • CDH12 Cadherin 12, Type 2 N-Cadherin 2 RPL5 Ribosomal

    CDH12 Cadherin 12, Type 2 N-Cadherin 2 RPL5 Ribosomal

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