Tuttnauer Model 5596 SP-1 @ FV
Glasswasher @ BRDG Autoclave & Sterilizers (Detect Instrument Picture to Jump to that section of SOP)
Bacticinerators and Bead Micro-Burner, Butane Primus Model A Sterilizer W RO water feed Prepared by: Bob Morrison @BRDG STLCC, Instrumentation Specialist
Dec 08, Last revision May 2015 (microburner) STLCC-CPLS:Morrison 5/27/2015 Page 1 Glasswasher; @BRDG, VWR Washer with DI Rinse and DI Pump, Spindle-Ready 97021-364
Feature stainless steel construction for long life and corrosion protection.
Multiple cycle selections and microprocessor control ensure that each load is processed efficiently and with the minimum energy required to meet cleaning requirements.
Operation is simple and loading is easy and flexible. A delayed-start feature allows the washers to operate automatically unattended during off-peak hours.
Uses a maximum of 15L (3.9gal.) of tap water. Low water requirement and precise detergent and neutralizer dosing minimize chemical costs
Spindle-ready models use an existing deionized water supply and can accommodate either one or two direct injection spindle racks to clean up to 44 flasks.
Volume: 170 L (6 cu. ft.) Exterior Dimensions, Undercounter Unit: 61W x 62D x 87–93H cm (24 x 241/2 x 341/4–361/2") Freestanding/Mobile Unit: 61W x 62D x 97.5H cm (24 x 241/2 x 383/8")(minimum height) Interior Dimensions: 55 x 48 x 64 cm (211/2 x 19 x 251/4") Clearance, Standard Upper Open Rack: 25.7 cm (101/8") Standard Lower Open Rack: 30.5 cm (12") Spindle Rack-Ready Upper Rack*: 19.7 cm (73/4") Spindle Rack-Ready Lower Rack*: 26 cm (101/4") Electrical: 115V, 60Hz, 15A *Maximum height with spindle clip at lowest position on spindle tube.
Link to Manf website, Optional equipment, details….
STLCC-CPLS:Morrison 5/27/2015 Page 2 Glasswasher: Control Panel, Detergent, @BRDG
PROG: Press to START; press set cycles; Max to Stop; Hold >3 sec Rinse only LED Displays Time remaining in cycle and current Temp.
High Heat Rinse High Heat Wash Button and LED Button and LED
Delayed Start and LED (above)
Detergent; Load main and prewash cups if desired
MASTER ON/OFF (Open Door to access)
STLCC-CPLS:Morrison 5/27/2015 Page 3 Glasswasher: Cycles/Phases, Settings
STLCC-CPLS:Morrison 5/27/2015 Page 4 Autoclave: Sterilizer, BRDG, Primus, Clinical Model A Emergency Clinical Model A, SN 17413, Primus Sterilizer Co. www.primus-sterilizer.com Power OFF 316L Stainless Steel Chamber with Standard PriMirror Interior Surface , Dimensions: 20”x20”x38” PSS500 Microcomputer Control Standard Vacuum, Gravity, Liquid and Test Cycles Single, Manual, Vertical Sliding Door Electric Steam Boiler, 208V, #4 ACW, 4 wire, 3ph, 67amps Cybertech Thermal Printer with Take Up Reel Drain Water Conservation Quench Includes Air Compressor
Safety Gloves
Loading cart/gurney Service: Link to Primus Clinical – 636-343-8928 Small Size Spec Sheet .pdf
STLCCInstall-CPLS:Morrison date @BRDG 5/27/2015 1/14/10 B-E Hospital Equipment Co, Inc. Page 5 Autoclave: BRDG, Primus, MTC Services Sep 2013
Clinical Model A, SN 17413, Primus Sterilizer Co. www.primus-sterilizer.com
Alternative Services CDS or ACE Lab Systems
STLCC-CPLS:Morrison 5/27/2015 Page 6 Autoclave: Primus, BRDG, Control Panel, Defining Cycles
Press buttons 1-4 to choose those cycles.
Press up-arrow and Displays current then 1-4 buttons to state or cycle access cycles 5-8 and parameters
Changing Cycle Parameters: 1. Press Cycle to be changed, then press MENU key, LED will light. ADV Key; resets 2. To change CycleType, press Enter, any operation then up arrow to see all types, press (Exit) Enter again to select a type. 3. To change other parameters on this OFF Key, press Cycle, use up arrow to scroll to the to stop next cycle parameter to be modified. operations 4. To change that value, Press Enter key, “asterisk” key then lights up. 5. Use up/down arrows to change the value (must be within allowable range Lights: Indicate see pg 14) current Cycle 6. Press ENTER key when done, then phase or activity continue up arrow to next parameter 7. Press ADV key to exit and return to the idle screen for normal operations.
STLCC-CPLS:Morrison 5/27/2015 Link to Primus Operator Manual .pdf Page 7 Autoclave: Starting a Cycle, Primus, BRDG
STARTING A CYCLE, be patient 1. Press the keypad for the desired cycle, the LED for that pad will turn on. For cycles above 4, press the up arrow and keypad numbers 1-4 (cycles 5-8)
2. Close the Sterilizer Door. You should see confirmation with the Door Closed LED.
3. Wait ..…..The “START” button be not be operative until the jacket temperature is within 6 F of the cycle operating temperature.
4. To view the Jacket temperature, press the Upper left hand heart on the Primus symbol.
5. When the jacket temperature has been reached, the READY and START LEDs will be lit.
6. Press the START button and hold for a few seconds to begin the cycle. The READY and START LED indicators will go out, Door button: may other cycle indicator LEDs will follow the be required to Cycle as it runs. release door after Page 8 aSTLCC cycle-CPLS:Morrison 5/27/2015 Autoclave: Primus, Typical Cycle Diagram, Liquids
STLCC-CPLS:Morrison 5/27/2015 Page 9 Autoclave: Primus, BRDG Modified Cycles
0
STLCC-CPLS:Morrison 5/27/2015 Page 10 Autoclave: Primus, Gravity Cycle Description
STLCC-CPLS:Morrison 5/27/2015 Page 11 Autoclave: Primus, Liquids Cycle Description
STLCC-CPLS:Morrison 5/27/2015 Page 12 Autoclave: Primus, Vacuum Cycle Description
STLCC-CPLS:Morrison 5/27/2015 Page 13 Autoclave: Primus, Cycle Sequences
STLCC-CPLS:Morrison 5/27/2015 Page 14 Autoclave: Primus, Parameter Range
STLCC-CPLS:Morrison 5/27/2015 Page 15 Autoclave: Safety Guidelines, Primus, BRDG 1. Never overload the Sterilizer, sterilization may never be achieved.
2. LIQUID cycles are only for the sterilization of Liquids, DO NOT USE ANY OTHER CYCLE for Liquids.
3. When processing LIQUIDS, use containers with vented closures..do not use ordinary glass bottles or any containers not designed for this purpose.
4. NEVER sterilize sealed containers in a cycle other than a LIQUIDS cycle, rapid pressure changes in other cycles can cause explosive results.
5. NEVER obstruct the drains in the bottom of the chamber, always load in a manner where no objects will touch or move to chamber walls.
6. ALWAYS use “hot gloves” when loading or unloading the sterilizer, before or after a cycle…. ALWAYS assume the door, walls, racks, and/or gurney are HOT!
7. ALWAYS place “hot gloves” on the ends of a loading cart to indicate to others that the cart and labware are HOT!
STLCC-CPLS:Morrison 5/27/2015 Page 16 WEEKLY – Execute this BLOW OUT protocol to Autoclave: Primus, BRDG, maintain boiler when unit is at operating Maintenance, Blow-down, condition Weekly (per Wally 11/28/11) 1. Turn OFF boiler (toggle switch on rear of boiler) 2. Turn OFF mail 208V power switch (left front wall) 3. Turn OFF autoclave (control panel) 4. Turn OFF Main water supply valve at rear wall 5. Unplug all three 110V power cords at rear 6. Remove Right side Autoclave Stainless Panel Blowdown line to 7. Using a protective glove locate Blow-Out Valve floor drain on other (Green) at lower rear side of the wall by 8. Open valve slowly about 30 degrees Millipore, steam exits counterclockwise there. 9. Observe water/steam exiting to drain on wall behind Millipore…. Wait until steam subsides 10. Open valve another 30 degrees, wait, and repeat again until valve is fully open (aligned with the exit pipe) 11. Return Valve to closed (pointing up) position 12. Turn ON main water supply valve 13. Turn ON main 208V switch and boiler toggle switch (left rear) 14. Re-insert 110V power plugs 15. Watch Glass tube on side of boiler to ensure it has refilled to acceptable level
16. Turn Autoclave back on 17. If RED Button (low boiler water level) on front panel is lighted (out), press to reset 18. Resume operations STLCC -CPLS:Morrison 5/27/2015 Page 17 Autoclave: Boiler Blowdown & Maintenance (from Boiler manual)
STLCC-CPLS:Morrison 5/27/2015 Page 18 Autoclave: Primus, Boiler Plumbing Diagram 1/4” Water Supply line
On/Off Master switch (access from rear of unit Water level glass tube
Drain/Pressure and Blowdown release valve
STLCC-CPLS:Morrison 5/27/2015 Page 19 Autoclave: Primus, PRI-Pure RO System Diagram 1/2
STLCC-CPLS:Morrison 5/27/2015 Page 20 Autoclave: Primus, PRI-Pure RO System Diagram 2/2
STLCC-CPLS:Morrison 5/27/2015 Page 21 Autoclave: Primus, Pri-Pure RO System
STLCC-CPLS:Morrison 5/27/2015 Page 22 Autoclave: Sterilizer, BRDG, Schematic Power 3 outlets Bldg Cold Compressed Bldg Cold Water 110V, 15Amp Water Feed Air (not Water Line Filter Feed Line to RO/Boiler used) non-boiler use
110v 110v 110v
Drain and Overflow Line RO Filters and 100L tank To Boiler Remove Panel to access Blow-out Valve (Weekly) Millipore DI water system (ref only, not connected) Primus Clinical Model A Autoclave 208V 3-Phase
Power Switch, On/Up position
Link to Primus Clinical – 208V Small Size Spec Sheet .pdf
Link to Primus Dwg .pdf For Boiler 208V, 67Amp B-E. Installation See Spec shts Jeremy Jamerson 314-680-4248
STLCC-CPLS:Morrison 5/27/2015 Page 23 Autoclave: Primus PRI-Pure RO System (Futuramics Clean Water, www.omahawater.com), to Boiler and Water Line Filter (added June 2012)
STLCC-CPLS:Morrison 5/27/2015 Page 24 Autoclave: Primus, Water Line-feed Filter (Grainger; Aqua-Pure)
Valve in Head, Single High, Grainger Item # 2P275 Size 3/4 In FNPT, Price (ea.) $124.75 Color Clear, Brand AQUA-PURE Mfr. Length 14 1/4 In, Width 4 9/16 In, Height 14 1/4 In, Model # AP101T Max. Temp. 100 F, 125 Max. Pressure (PSI), Dia. 4 9/16 In., Reduces Dirt And Rust, Plastic Housing Material, HotLink to Aqua-Pure Manual .pdf Cartridges Required 1 Includes 1P753 Cartridge
Water Filter Cartridge, Material of Construction Melt Blown Polypropylene, Rating 5 Microns, Application Rust and Dirt, Flow 8 GPM, Recommended by Jeremy of B&E Height 9 3/4 Inches, Diameter 2 5/8 Inches Grainger Item # 1P753 4/26/12 for non-boiler related water use Price (pk.) $25.55 Package Qty. 2 Brand AQUA-PURE Mfr. Model # AP110
STLCC-CPLS:Morrison 5/27/2015 http://www.aquapurefilters.com/store/product/00026.200018/ap101t.html Page 25 Autoclave: Water Line Feed, AP101T, Filter Replacement, every 6mo
STLCC-CPLS:Morrison 5/27/2015 Page 26 Autoclave: Primus, Print Interval Setting
STLCC-CPLS:Morrison 5/27/2015 Page 27 Autoclave: Primus, Loading Thermal Paper
First : Remove the Take-up Reel and SAVE the take-up reel by pushing it out of the spool.
Paper Can be Ordered from Supply Concepts: Item #: SPR-25347 $2.24 Sparco Co. Thermal Paper Roll, 2-1/4"x85', 3/PK,
Or OfficeMax: Item# 20045997 Manf# OM98105 Thermal 2 1/4" x 85' 12rolls/pk $19.99
STLCC-CPLS:Morrison 5/27/2015 Page 28 Autoclave: Primus, Loading Printer Take-up Spool
Recommended Method (BobM) 1. Place empty spool in the area of the hubs 2. Use a screwdriver to open/expand the width of the open slot to make it easier to snap over the hub ends 3. When empty spool is in place, fold paper into a “V”, insert into spool slot 4. Press FEED to take up some paper on spool.
STLCC-CPLS:Morrison 5/27/2015 Page 29 Autoclave: Thermometer, Temp Max after cycle
For use in autoclaves without built-in temperature recording devices
Has constricted capillary to hold temperature after heat is reduced so you can monitor maximum temperature reached during sterilization.
Thermometer, Autoclave; Fisherbrand; Length: 200mm; Total immersion; Sun division: 0.5°C; Range: 85° to 135°C; Contains mercury
Fisher Sci: CAT # 15-114-10 Each for $66.95
STLCC-CPLS:Morrison 5/27/2015 Page 30 Autoclave: Temperature Loggers (example) Omega.com Specifications Temperature Sensor: 100Ω Platinum RTD Autoclave Temperature Data Logger Temperature Range (Body): -40 to 140ºC (-40 to 284ºF) OM-CP-HITEMP140 , Approx $499 Temperature Measurement Range (Probe): -200 to 260ºC (-328 to 500ºF) Temperature Resolution: 0.01ºC (0.02ºF) Calibrated Accuracy: ±0.1ºC (±0.18ºF) [-20 to 140ºC (-4 to 284ºF)] Start Modes: • Software programmable immediate start • Delay start up to eighteen months in advance Real Time Recording: May be used with PC to monitor and record data in real time Memory: 32,700 readings Reading Rate: One second up to once every 24 hours Battery Type: 3.6V high-temperature lithium battery (included) Battery Life: 1 year typical [1 minute reading rate at 25ºC (77ºF)] Calibration: Digital calibration through software Calibration Date: Automatically recorded within device OM-CP-HITEMP140 3 Weeks $499.00 Data Format: Date and time stamped ºC, ºF, K, ºR Autoclave temperature data logger qty. Time Accuracy: ±1 minute/month at 20 to 30ºC (68 to 86ºF) (RS-232 cable not in use) OM-CP-HITEMP140-CERT $559.00 Computer Interface: OM-CP-IFC400 USB docking station Autoclave temperature data logger and NIST certificate required 125,000 baud qty. Software: XP SP3/Vista/Windows® 7 (32- and 64-bit) Operating Environment: -40 to 140ºC (-40 to 284ºF), 0 to 100% OM-CP-IFC400 $120.00 RH Docking station with USB cable, software and manual Dimensions qty. Body: 67 H x 25 mm D (2.7 x 0.9") Probe: 50 L x 3.8 to 3.2 mm transitional dia (2.0 x 0.188 to OM-CP-BAT110 $50.00 0.125") Replacement 3.6 V high-temperature lithium battery Weight: 120 g (4.2 oz) Material: 316 stainless steel STLCC-CPLS:Morrison 5/27/2015 Page 31 Autoclave FV ; General Information Link to B-E Hospital Equip Co, Inc . STL sales/service Wally Rodemacher tech Spec. 314.241.0855
Description: Tuttnauer 5596 SP -1 Sterilizer
Dimensions: 43” W x 56” D x 75” H (External Stainless walls with enclosed boiler and pump. )
Requires: DI and standard cold water input and 1.5” exhaust/vapor line.
Clearances: 500mm on sides and rear. Front should have clear access for trays on carts.
• Chamber size is 15" dia x 29.5" deep. Chamber Dimensions WxDxH mm (inc.): 508 x 508 x 970 Chamber Volume (Liters): 250 Total Weight with all components 1000 KG
• High speed, high vacuum, high capacity, hospital grade sterilizer with FDA cleared flash cycle • A built in vacuum pump and steam generator allows for increased load size and dramatically reduces total cycle time. • The closed door drying system provides a faster and more thorough drying cycle. Seven automatic cycles are available. • Five sterilization cycles and two test cycles. • Designed for sterilization of medical and surgical materials such as packed and wrapped solids and instruments. STLCC -CPLS:Morrison 5/27/2015 Page 32 Autoclave: FV-Tuttnauer Model 5596
Autoclave: General Information from: David Morganstern Sales Manager tuttnauer USA 25 Power Drive Hauppauge, NY 11788Ph: 800-624-5836 x112 Fax: 631-737-1034 E-mail: [email protected]
Link to General Notes for Architects/Contractors .pdf
Link to Installation and Clearance Drawing .pdf
• Chamber Size: 20x20x38 with Manual Hinged Door • Chamber Volume: 250 Liters • External Dimensions: 30”W x 56”D x 75”H • Carbon Steel Electric Steam Generator (Integral) • Free-Standing with Stainless Steel Cabinet Enclosure • Standard Cycles: (4)Gravity, (4)Pre-Vac, (4)Liquid and (2)Test Cycles • Utilities required (by others): Water (approx. 50Mohm), 3-phase electric, Compressed air, Floor Drain • Designed for sterilization of porous and non-porous material, liquids, glassware etc. • Warranty: 1 year parts and labor, 15 years on the pressure vessel • Accessories: Rack with two shelves Options not included: • Integral air compressor (if house air is not available) • Auto- Vertical Sliding Door (Model 5596 ECP-V) STLCC-CPLS:Morrison 5/27/2015 Page 33 Autclave FV; Inside Panel Views; Tuttnauer 5596
Boiler for Steam/Pressure
RODI Water storage from Millipore system
Air Compressor and tank; out for
STLCC-CPLS:Morrison 5/27/2015 service goes into lower bay Page 34 Autoclave : Tuttnauer Basic Operating Instructions 1. Open the main steam inlet valve and watch the jacket pressure gauge on the front panel. When the jacket pressure reaches 2.3 bars, the sterilization cycle can be started. (condensate may exit through the jacket steam trap during this period) 2. Open the door by turning the control wheel handle counter clockwise to release the locking arms. 3. Load the material to be sterilized per the guidelines in this protocol 4. Close the door by pushing towards the frame and turning the wheel handle clockwise until the “Door Closed” lamp is lit. 5. On the control panel, chose the sterilization program according to the materials loaded. The light for the program should be on with parameters displayed. 6. Press the START key to begin the cycle. Lights on the bottom indicate the stage of the cycles in progress. – Air is purged from the chamber at the beginning of the sterilization cycle. – Steam under pressure is introduced into the chamber and the temperature rises to the desired level. – The autoclave is maintained under pressure at a high temperature for a predetermined time. – Steam is then exhausted from the chamber at the end of the cycle. 7. A buzzer will sound and the START light will turn off at the end of the cycle. The printer will print a summary of the cycle. 8. Open the door by turning the wheel handle counter clockwise. 9. Remove sterilized materials carefully using orange or heat resistant gloves
STLCC-CPLS:Morrison 5/27/2015 Page 35 Autoclave : Tuttnauer Safety Features and Audible Alarms 1. Steel bar Door Locks 2. Safety Vent/Vent Valve • Safety valve – opens as back-up protection to reduce chamber pressure in the event of exceeding 262 kPa • Vent Valve – needed if chamber pressure should exceed 241kPa, the vent valve will open and the P-2 alarm will display.
3. Always use “HOT GLOVES” (orange) when removing items from the Autoclave
1. Overheat Protection – chamber temperature is set so as not to exceed 149 C and has additional overheat protection if temperature reaches 180 C.
2. Electrical power interruption – in case of an electrical power failure during the sterilization cycle, pressure in the chamber is automatically vented to the atmosphere.
3. Audible Signals – Each time a switch is depressed one beep occurs (except when depressing the four arrows). – Five beeps indicates sterilization cycle is complete. – Five beeps indicates drying cycle is complete. – Six beeps operational error or alarm. – Continuous beeps – door is open during fill cycle. Page 36 STLCC -CPLS:Morrison 5/27/2015
• Unwrapped Instruments (without drying stage) Autoclave : Tuttenaeur • Wrapped Instrumentss (with drying stage) Control and Monitoring Keys • Liquids; pressing one of the program keys determines the stages to be run • Vacuum test/Bowie Dick test; press once for Vacuum, twice for Bowie test • TEMP; shows sterilization temperature. Press UP/DN keys to permanently change this value from the nominal setting. • STE Time; shows sterilization time. Use UP/DN to permanently alter this setting. • DRY Time; shows drying time. Alter with UP/DN keys. • Clock ; Press to change settings with UP/DN keys. • START; begins selected sterilization program. Will not start if door in not secured. • STOP; Only key that can be used to stop operations once a program cycle has begun.
PROGRAM CYCLE or STATUS
STLCC-CPLS:Morrison 5/27/2015 Page 37 Autoclave: Tuttnauer Sterilizing Media in Flask; Setup
1. Make sure pressure Jacket pressure is at or above 18 psi
2: Select Liquids on control panel
(prepared from: E. Lyons 10/22/08) STLCC-CPLS:Morrison 5/27/2015 Page 38 Autoclave: Tuttnauer Sterilizing Media in Flask; Run Settings, Door operations 3. Check to make sure that the autoclave is set to run for 20 minutes at 250F. (The photo shows that this is program 12, and the temperature and time are set correctly.)
4. Open the autoclave door by turning counter clockwise. Place the flasks in the autoclave with some room between them. Close the door tightly with clockwise motion.
Open Door: Counter Clockwise CCW
Close Door: Clockwise CW (prepared from: E. Lyons 10/22/08) STLCC-CPLS:Morrison 5/27/2015 Page 39 Autoclave: Tuttnauer Sterilizing Media in Flask ; Start and Run Verify 5. Press the START button on the control panel.
6. Check to make sure that the autoclave has functioned properly during the heating cycle by checking the recording tape for errors. (This tape can be copied for inclusion in your lab notebook.)
(prepared from: E. Lyons 10/22/08) STLCC-CPLS:Morrison 5/27/2015 Page 40 DATE 4/13/2009 TIME 4:36:07 Autoclave: Tuttnauer Run Print Tape PROG Liqud1 TEMP 250F Examples TIME 20 min DRY 00 min PROGS: Liqud1, UnWrp1, UNWrp3 EXH MODE 4 END TEMP 203 DATE 4/13/2009 MN TEMP PRESS TIME 4:36:07 V01 231F 15P PROG UnWrp1 V02 201F 15I TEMP 250F S04 250 17P TIME 20 min S05 252 17P DRY 10Min S06 252 17P EXH MODE 1 S07 252 17P MN TEMP PRESS S08 252 17P V01 231F 15P S09 252 17P V03 191 15I …. … … S04 250 17P S23 252 17P S05 252 17P S24 251 16P S06 252 17P E25 247 13P S07 253 17P DATE 4/13/2009 E28 229 04P S08 252 16P TIME 4:36:07 E31 218 00P …. … … PROG UnWrp3 E34 215 00P S18 255 18P TEMP 270F E37 210 00P E20 237 03P TIME 05 Min E40 210 01I D20 183 23I DRY 00Min E43 210 01I D22 197 22I EXH MODE 1 E46 210 01I D28 168 23I MN TEMP PRESS E49 204 01I D30 162 22I F08 079F 01P E49 204 01I O.K. F09 079F 01P O.K. Operator Operator Operator Load NO 7 Load NO. 2 Load NO 5 Autoclave No. 12 Autoclave No. 12 Autoclave No. 12
STLCC-CPLS:Morrison 5/27/2015 Page 41
Autoclave: Sterilizing Media in Flask ; Removing and Cooling
7a. When the autoclave is finished and the inside pressure has dissipated, remove your flask of media using orange hot gloves. 7b. Swirl the media gently because the agar may settle to the bottom of the flask during autoclaving.
8a. Place the media into a water bath set at 55-60C, which is the temperature it should cool to for pouring the plates. (Note: If the solution is not placed in a water bath, cool until you can just hold your bare hands on the bottom of the flask. 8b. If it begins to solidify, it will have to be re-melted by autoclaving again for about 5 minutes.
(prepared from: E. Lyons 10/22/08) STLCC-CPLS:Morrison 5/27/2015 Page 42 Autoclave: Sterilization Process and Verification
• Sterilization – the highest level of disinfection; the killing of all living organisms on a surface
• Testing whether sterilization has occurred during autoclaving is done using spores (dormant microorganisms) – Spore strips are dried pieces of paper to which large number of pathogenic , heat-resistant Bacillus thermopholus spores are adhered. – After autoclaving the strips are transferred to growth medium, if successful autoclaving, then the spores will not grow.
STLCC-CPLS:Morrison 5/27/2015 Page 43 Autoclaves: Cycles, Gravity vs. Vacuum
Gravity Cycles: Displacement Autoclaves – The Old Workhorse
Traditional models rely upon gravity to displace the air from the sterilizing chamber. Steam is much lighter than air and, as it is injected into the pressure chamber, it forces air downwards, trapping it in a sink. A system of valves and taps bleed out this excess air, allowing the steam to sterilize the contents of the chamber.
These autoclaves are the cheapest option and they are very easy to use. As a result, they are common in kitchens and manicurists, where they are more than adequate. However, for sterilizing porous or wrapped materials, displacement autoclaves are not efficient enough. Hot air may be trapped inside, allowing pockets of microbes and other pathogens to evade the sterilization cycle.
Vacuum Autoclaves – The Safer Solution to Sterilization
For complex sterilization, or in areas where complete sterility is necessary, a vacuum autoclave is the superior choice. Vacuum autoclaves evacuate the air, with a vacuum, and steam is then injected into the chamber. This allows the steam to penetrate porous materials, wiping out any pockets of pathogens in hard-to-reach areas.
After this steam sterilization stage, the pump again evacuates the chamber, drying the autoclave contents thoroughly. This added stage prevents germs and prions being retransmitted by trapped moisture. Vacuum autoclaves are the preferred option in medical institutions, as a more effective alternative to the simpler displacement autoclaves.
Many hospitals use disposable medical equipment, to prevent the transmission of CJD variants, but the contaminated waste is still autoclaved before landfill. Autoclaving is a more environmentally friendly method than traditional incineration, which ejects toxic gases into the atmosphere.
STLCC-CPLS:Morrison 5/27/2015 Page 44 Autoclave; Tuttnauer 5596 SP Series
STLCC-CPLS:Morrison 5/27/2015 Page 45 Autoclave : Tuttnauer Utilities Consumption
• 16”x16”x28” 20”x20”x38” • H44 • D • W • H • D • W • H • D
H55 Series
STLCC-CPLS:Morrison 5/27/2015 Page 46 Autoclave/Sterilizer: BRDG Proposal, STERIS/AMSCO-Lab 250
The Amsco Lab 250 sterilizer was designed with the needs and the budget of the laboratory research market in mind. The lab series sterilizer provides the same time-proven, safe, reliable chamber and stand assembly as the Century® sterilizer that has been offered to the healthcare and life sciences markets since 1992.
The Amsco Lab 250 features an Allen-Bradley MicroLogix" control and a full-color operator interface. The unit has a chamber size of 20 x 20 x 38 inches (508 x 508 x 965 mm) and is equipped with gravity and liquid type cycles as standard. Optional prevacuum and isothermal cycles are also available if required. A manually operated vertical sliding door is combined with the non-lubricated door seal for reliability and ease of use. The drain line piping package meets or exceeds the maximum water temperature requirements of the industry - 60ºC (140ºF).
Link to AMSCO Lab 250 Brochure…. pdf
Link to AMSCO Lab 250 Spec Sheets…. pdf
Link to AMSCO Lab 250 Tech Sheets…. pdf
Page 47 STLCC-CPLS:Morrison 5/27/2015 Autoclave/Sterilizer: BRDG Proposal, AMSCO Lab 250 Spec Sheet
STLCC-CPLS:Morrison 5/27/2015 Page 48 Autoclave/Sterilizer: BRDG Proposal, AMSCO Lab 250 Spec Sheet
STLCC-CPLS:Morrison 5/27/2015 Page 49 Sterilizer: Bacti-Cinerator
• Sterilizes loops and needles safely and conveniently.
• Prevents infectious spatter and/or cross contamination.
• Safeguard laboratory personnel -no open flame -no hazardous gas.
• Ideal for use in anaerobic chambers.
• Core Tube does not contain asbestos
On/off toggle switch
Heat element “on” indicator
Oxford® Bacti-Cinerator® IV (Cole-Palmer) EW-01850-26 Replacement Heating Element for 01850-22 (120V)
STLCC-CPLS:Morrison 5/27/2015 Page 50 Sterilizer; Bacti Cinerator- Features, Safety
Core will glow red-hot when approx 815C temp has been reached
Extremely Hot surface. Do not touch or allow other items to come in contact
• Allow 6-10 minutes for the Bacti-Cinerator to reach an optimum sterilizing temperature of 815°C (1500°F) throughout the interior of the core. • 5-7 seconds of exposure sterilizes loops, needles and culture tubes. Unit is not intended for use with scalpels, forceps or sharp instruments. • Use only insulated loop holders when inserting loops into the heater element. • Suitable for anaerobic procedures in chambers and hoods. • Yellow Light indicates that the heating element is ON. Note, the Core element may remain very hot for 30 minutes after it is turned off. Allow 1 hour of cooling before cleaning or storage. • Avoid any contact with the heat shield or end caps while the Sterilizer is on. • Do not immerse the sterilizer in water or other cleaning solutions. • Periodically inspect the element for cracks (yellow-orange fissures) and wear.
Do not wrap power cord around
STLCC-CPLS:Morrison 5/27/2015 Heat element for storage. Page 51
Sterilizer: Bead
Effectively sterilize small, solid metal and glass implements in seconds
• Heated glass beads destroy all microbes and spores
• Low power consumption allows for all day operation
• Safe to use—no gases, fumes, open flames or harsh liquids
STLCC-CPLS:Morrison 5/27/2015 Page 52 Bead Sterilizer: Setup and Operating Protocol
1. Sterilizer Usage: • Remove the metal cover cap before turning on the unit • Allow 15 minutes to reach 240C before use • Unit can be left on for further use • Turn off at the end of the day • Replace metal cap when cooled to prevent contamination 2. Caution: Do not touch beads or sides of container when in use. 3. Small Dish: • Use for small metal objects • Cover dish with metal cap for 3 minutes maximum • Remove dish and cap when finished 4. Glass beads are heated to 250°C to effectively destroy all microbes and spores. Suitable for sterilizing scissors, forceps, scalpels, inoculating loops and needles. 5. Power consumption is low allowing for all day operation. 6. Dial thermometer on front of sterilizer ensures proper heating and back-up safety thermostat prevents over-heating.
STLCC-CPLS:Morrison 5/27/2015 Page 53 Sterilizer/Heat Source- Micro Burner Butane This small butane burner uses clean burning butane and features a trigger ignition, eliminating the need for wicks, matches or a central gas line. Avg use rate 2oz/hr, refill about every 45min
STLCC-CPLS:Morrison 5/27/2015 Page 54 Sterilizer/Heat Source- Micro Burner Butane
STLCC-CPLS:Morrison 5/27/2015 Page 55