Laboratory Glassware for Your Lab 2006
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Use of Laboratory Equipement
USE OF LABORATORY EQUIPEMENT C. Laboratory Thermometers Most thermometers are based upon the principle that liquids expand when heated. Most common thermometers use mercury or colored alcohol as the liquid. These thermometers are constructed as that a uniform-diameter capillary tube surmounts a liquid reservoir. To calibrate a thermometer, one defines two reference points, normally the freezing point of water (0°C, 32°F) and the boiling point of water (100°C, 212°F) at 1 tam of pressure (1 tam = 760 mm Hg). Once these points are marked on the capillary, its length is then subdivided into uniform divisions called degrees. There are 100° between these two points on the Celsius (°C, or centigrade) scale and 180° between those two points on the Fahrenheit (°F) scale. °F = 1.8 °C + 32 The Thermometer and Its Calibration This section describes the proper technique for checking the accuracy of your thermometer. These measurements will show how measured temperatures (read from thermometer) compare with true temperatures (the boiling and freezing points of water). The freezing point of water is 0°C; the boiling point depends upon atmospheric pressure but at sea level it is 100°C. Option 1: Place approximately 50 mL of ice in a 250-mL beaker and cover the ice with distilled water. Allow about 15 min for the mixture to come to equilibrium and then measure and record the temperature of the mixture. Theoretically, this temperature is 0°C. Option 2: Set up a 250-mL beaker on a wire gauze and iron ring. Fill the beaker about half full with distilled water. -
Laboratory Glassware N Edition No
Laboratory Glassware n Edition No. 2 n Index Introduction 3 Ground joint glassware 13 Volumetric glassware 53 General laboratory glassware 65 Alphabetical index 76 Índice alfabético 77 Index Reference index 78 [email protected] Scharlau has been in the scientific glassware business for over 15 years Until now Scharlab S.L. had limited its sales to the Spanish market. However, now, coinciding with the inauguration of the new workshop next to our warehouse in Sentmenat, we are ready to export our scientific glassware to other countries. Standard and made to order Products for which there is regular demand are produced in larger Scharlau glassware quantities and then stocked for almost immediate supply. Other products are either manufactured directly from glass tubing or are constructed from a number of semi-finished products. Quality Even today, scientific glassblowing remains a highly skilled hand craft and the quality of glassware depends on the skill of each blower. Careful selection of the raw glass ensures that our final products are free from imperfections such as air lines, scratches and stones. You will be able to judge for yourself the workmanship of our glassware products. Safety All our glassware is annealed and made stress free to avoid breakage. Fax: +34 93 715 67 25 Scharlab The Lab Sourcing Group 3 www.scharlab.com Glassware Scharlau glassware is made from borosilicate glass that meets the specifications of the following standards: BS ISO 3585, DIN 12217 Type 3.3 Borosilicate glass ASTM E-438 Type 1 Class A Borosilicate glass US Pharmacopoeia Type 1 Borosilicate glass European Pharmacopoeia Type 1 Glass The typical chemical composition of our borosilicate glass is as follows: O Si 2 81% B2O3 13% Na2O 4% Al2O3 2% Glass is an inorganic substance that on cooling becomes rigid without crystallising and therefore it has no melting point as such. -
PML Brochure
PHYSICAL MEASUREMENT LABORATORY Gauging nature on all scales NIST.GOV/PML PHYSICAL MEASUREMENT LABORATORY (PML) The Physical Measurement Laboratory (PML), a major frequency, electricity, temperature, humidity, pressure operating unit of the National Institute of Standards and vacuum, liquid and gas flow, and electromag- and Technology (NIST), sets the definitive U.S. standards netic, optical, acoustic, and ionizing radiation. PML for nearly every kind of measurement in modern life, collaborates directly with industry, universities, profes- sometimes across more than 20 orders of magni- sional and standards-setting organizations, and other tude. PML is a world leader in the science of physical agencies of government to ensure accuracy and to measurement, devising procedures and tools that make solve problems. It also supports research in many continual progress possible. Exact measurements are fields of urgent national importance, such as manu- absolutely essential to industry, medicine, the research facturing, energy, health, law enforcement and community, and government. All of them depend on homeland security, communications, military defense, PML to develop, maintain, and disseminate the official electronics, the environment, lighting and display, standards for a wide range of quantities, including radiation, remote sensing, space exploration, length, mass, force and shock, acceleration, time and and transportation. IMPACTS ❱ Provides 700 kinds of calibration services ❱ Numerous special testing services NIST.GOV/PML | 2 NIST.GOV/PML -
Chemostat Culture for Yeast Experimental Evolution
Downloaded from http://cshprotocols.cshlp.org/ at Cold Spring Harbor Laboratory Library on August 9, 2017 - Published by Cold Spring Harbor Laboratory Press Protocol Chemostat Culture for Yeast Experimental Evolution Celia Payen and Maitreya J. Dunham1 Department of Genome Sciences, University of Washington, Seattle, Washington 98195 Experimental evolution is one approach used to address a broad range of questions related to evolution and adaptation to strong selection pressures. Experimental evolution of diverse microbial and viral systems has routinely been used to study new traits and behaviors and also to dissect mechanisms of rapid evolution. This protocol describes the practical aspects of experimental evolution with yeast grown in chemostats, including the setup of the experiment and sampling methods as well as best laboratory and record-keeping practices. MATERIALS It is essential that you consult the appropriate Material Safety Data Sheets and your institution’s Environmental Health and Safety Office for proper handling of equipment and hazardous material used in this protocol. Reagents Defined minimal medium appropriate for the experiment For examples, see Protocol: Assembly of a Mini-Chemostat Array (Miller et al. 2015). Ethanol (95%) Glycerol (20% and 50%; sterile) Yeast strain of interest Equipment Agar plates (appropriate for chosen strain) Chemostat array Assemble the apparatus as described in Miller et al. (2013) and Protocol: Assembly of a Mini-Chemostat Array (Miller et al. 2015). Cryo deep-freeze labels Cryogenic vials Culture tubes Cytometer (BD Accuri C6) Glass beads, 4 mm (sterile; for plating yeast cells) Glass cylinder Kimwipes 1Correspondence: [email protected] © 2017 Cold Spring Harbor Laboratory Press Cite this protocol as Cold Spring Harb Protoc; doi:10.1101/pdb.prot089011 559 Downloaded from http://cshprotocols.cshlp.org/ at Cold Spring Harbor Laboratory Library on August 9, 2017 - Published by Cold Spring Harbor Laboratory Press C. -
Q Path Adhesive Slide
In Vitro Diagnostic Medical Device For professional use only Q PATH ADHESIVE SLIDE According to standard ISO 18113-2 : 2009, Point 7 Requirements for instructions or use and consolidated Directive 98/79/EC PRODUCT NAME Cat. No Description Pack Size 10048001 Q path Adhesive Slide white Box of 72 slides 10048002 Q path Adhesive Slide blue Box of 72 slides 10048003 Q path Adhesive Slide green Box of 72 slides 10048004 Q path Adhesive Slide yellow Box of 72 slides 10048005 Q path Adhesive Slide pink Box of 72 slides INTENDED PURPOSE These microscope slides may be used for the routine diagnosis of suspensions of cells and samples of tissue. Given that the possibilities for their use are highly varied, these glass slides should only be handled by users who hold a professional qualification that complies with national legislation. These microscope slides are intended for single use only. Any re-use or inappropriate treatment of their surface could lead to inaccurate results, the deterioration of preparations, and erroneous diagnoses. WARNING AND PRECAUTIONS Before use, please read all this information carefully. Microscope slides and cover slips are intended for single use by qualified personnel. They must under no circumstances be used more than once, and must be disposed of after use or at the end of the archive period in an appropriate manner as potentially infectious waste. If you observe any damage to the packaging, or any signs of glass fragments, the slides and cover slips must not be used, due to the risk of injury from glass splinters. Recommendation: In order to reduce the risk of injury due to sharp edges as much as possible, we strongly recommend the use of our microscope slides with polished edges. -
Uv and Visible Spectroscopy1
Page 1 of 4 I. INTRODUCTION TO UV AND VISIBLE SPECTROSCOPY1 When white light passes through or is reflected by a colored substance, a characteristic portion of the mixed wavelengths is absorbed. The remaining light will then assume the complementary color to the wavelength(s) absorbed. Thus, absorption of 420-430 nm light renders a substance yellow, and absorption of 500-520 nm light makes it red. Green is unique in that it can be created by absorption close to 400 nm as well as absorption near 800 nm. When sample molecules are exposed to light having an energy that matches a possible electronic transition within the molecule, some of the light energy will be absorbed as the electron is promoted to a higher energy orbital. An optical spectrometer records the wavelengths at which absorption occurs, together with the degree of absorption at each wavelength. The resulting spectrum is presented as a graph of absorbance (A) versus wavelength. Absorbance usually ranges from 0 (no absorption) to 2 (99% absorption), and is precisely defined in context with spectrometer operation. Because the absorbance of a sample will be proportional to the number of absorbing molecules in the spectrometer light beam (e.g. their molar concentration in the sample tube), it is necessary to correct the absorbance value for this and other operational factors if the spectra of different compounds are to be compared in a meaningful way. The corrected absorption value is called "molar absorptivity", and is particularly useful when comparing the spectra of different compounds and determining the relative strength of light absorbing functions (chromophores). -
BROOKFIELD DIAL READING VISCOMETER with Electronic Drive
BROOKFIELD DIAL READING VISCOMETER with Electronic Drive Operating Instructions Manual No. M00-151-I0614 SPECIALISTS IN THE MEASUREMENT AND CONTROL OF VISCOSITY with offices in : Boston • Chicago • London • Stuttgart • Guangzhou BROOKFIELD ENGINEERING LABORATORIES, INC. 11 Commerce Boulevard, Middleboro, MA 02346 USA TEL 508-946-6200 or 800-628-8139 (USA excluding MA) FAX 508-946-6262 INTERNET http://www.brookfieldengineering.com TABLE OF CONTENTS I. INTRODUCTION .....................................................................................5 I.1 Components .......................................................................................................5 I.2 Utilities ................................................................................................................6 I.3 Specifications .....................................................................................................6 I.4 Set-Up ................................................................................................................7 I.5 IQ, OQ, PQ .........................................................................................................7 I.6 Safety Symbols and Precautions .......................................................................8 I.7 Cleaning .............................................................................................................8 II. GETTING STARTED ..............................................................................9 II.1 Operation ...........................................................................................................9 -
ELISA Plate Reader
applications guide to microplate systems applications guide to microplate systems GETTING THE MOST FROM YOUR MOLECULAR DEVICES MICROPLATE SYSTEMS SALES OFFICES United States Molecular Devices Corp. Tel. 800-635-5577 Fax 408-747-3601 United Kingdom Molecular Devices Ltd. Tel. +44-118-944-8000 Fax +44-118-944-8001 Germany Molecular Devices GMBH Tel. +49-89-9620-2340 Fax +49-89-9620-2345 Japan Nihon Molecular Devices Tel. +06-6399-8211 Fax +06-6399-8212 www.moleculardevices.com ©2002 Molecular Devices Corporation. Printed in U.S.A. #0120-1293A SpectraMax, SoftMax Pro, Vmax and Emax are registered trademarks and VersaMax, Lmax, CatchPoint and Stoplight Red are trademarks of Molecular Devices Corporation. All other trademarks are proprty of their respective companies. complete solutions for signal transduction assays AN EXAMPLE USING THE CATCHPOINT CYCLIC-AMP FLUORESCENT ASSAY KIT AND THE GEMINI XS MICROPLATE READER The Molecular Devices family of products typical applications for Molecular Devices microplate readers offers complete solutions for your signal transduction assays. Our integrated systems γ α β s include readers, washers, software and reagents. GDP αs AC absorbance fluorescence luminescence GTP PRINCIPLE OF CATCHPOINT CYCLIC-AMP ASSAY readers readers readers > Cell lysate is incubated with anti-cAMP assay type SpectraMax® SpectraMax® SpectraMax® VersaMax™ VMax® EMax® Gemini XS LMax™ ATP Plus384 190 340PC384 antibody and cAMP-HRP conjugate ELISA/IMMUNOASSAYS > nucleus Single addition step PROTEIN QUANTITATION cAMP > λEX 530 nm/λEM 590 nm, λCO 570 nm UV (280) Bradford, BCA, Lowry For more information on CatchPoint™ assay NanoOrange™, CBQCA kits, including the complete procedure for this NUCLEIC ACID QUANTITATION assay (MaxLine Application Note #46), visit UV (260) our web site at www.moleculardevices.com. -
Laboratory Equipment Reference Sheet
Laboratory Equipment Stirring Rod: Reference Sheet: Iron Ring: Description: Glass rod. Uses: To stir combinations; To use in pouring liquids. Evaporating Dish: Description: Iron ring with a screw fastener; Several Sizes Uses: To fasten to the ring stand as a support for an apparatus Description: Porcelain dish. Buret Clamp/Test Tube Clamp: Uses: As a container for small amounts of liquids being evaporated. Glass Plate: Description: Metal clamp with a screw fastener, swivel and lock nut, adjusting screw, and a curved clamp. Uses: To hold an apparatus; May be fastened to a ring stand. Mortar and Pestle: Description: Thick glass. Uses: Many uses; Should not be heated Description: Heavy porcelain dish with a grinder. Watch Glass: Uses: To grind chemicals to a powder. Spatula: Description: Curved glass. Uses: May be used as a beaker cover; May be used in evaporating very small amounts of Description: Made of metal or porcelain. liquid. Uses: To transfer solid chemicals in weighing. Funnel: Triangular File: Description: Metal file with three cutting edges. Uses: To scratch glass or file. Rubber Connector: Description: Glass or plastic. Uses: To hold filter paper; May be used in pouring Description: Short length of tubing. Medicine Dropper: Uses: To connect parts of an apparatus. Pinch Clamp: Description: Glass tip with a rubber bulb. Uses: To transfer small amounts of liquid. Forceps: Description: Metal clamp with finger grips. Uses: To clamp a rubber connector. Test Tube Rack: Description: Metal Uses: To pick up or hold small objects. Beaker: Description: Rack; May be wood, metal, or plastic. Uses: To hold test tubes in an upright position. -
Kimblecatalog Dwkcover Digital.Pdf
1 ADAPTERS 223 GAS SAMPLING 16 AMPULES 226 HYDROMETERS 16 ARSINE GENERATORS 229 ISO 17 BEADS 235 JARS 18 BEAKERS 236 JUGS 21 BOTTLES 237 KITS AND LABSETS 41 BURETS 265 NMR 47 CAPS, CLOSURES, SEPTA 271 PETROCHEMICAL 59 CELL CULTURE 289 PIPETS 62 CENTRIFUGE TUBES 294 PURGE AND TRAP 71 CHROMATOGRAPHY 295 RAY-SORB 112 CLAMPS 300 ROTARY EVAPORATORS 114 CONCENTRATORS 305 SAFETY 118 CONDENSERS 319 SERIALIZED AND CERTIFIED 124 CONES 324 SLEEVES 124 CRUCIBLES 324 STARTER PACKS 125 CYLINDERS 325 STIRRERS 131 DAIRYWARE 328 STOPCOCKS AND VALVES 135 DESICCATORS 337 STOPPERS 135 DISHES 340 TISSUE GRINDERS 136 DISPENSERS 348 TUBES 136 DISSOLUTION VESSELS 365 VACUUM AND AIRLESS 137 DISTILLATION 374 VIALS 158 DRYING 389 WASHERS 159 EXTRACTION 390 WEIGHING BOATS 165 FILTRATION 391 TECHNICAL INFORMATION 179 FLASKS 433 INDEX TABLE OF CONTENTS TABLE 210 FREEZE DRYING 210 FRITTED WARE 211 FUNNELS Kimble has the products and expertise to support our customers’ workflows. We focus on providing laboratory glassware solutions from sample storage to sample disposition for market segments such as petrochemical, pharma/biotech/life sciences, environmental and food/beverage. With our breadth of products and depth of knowledge, Kimble offers everything you need to streamline your workflow and simplify everyday life in the lab. From vials and NMR tubes to barcoding services and beakers, we’ve got you—and your sample— covered from start to finish. Discover why Every Sample Deserves Kimble Sample Collection Sample Storage Sample Preparation Detection Sample Disposition -
Information Technology Laboratory Technical Accomplishments
CONTENTS Director’s Foreword 1 ITL at a Glance 4 ITL Research Blueprint 6 Accomplishments of our Research Program 7 Foundation Research Areas 8 Selected Cross-Cutting Themes 26 Industry and International Interactions 36 Publications 44 NISTIR 7169 Conferences 47 February 2005 Staff Recognition 50 U.S. DEPARTMENT OF COMMERCE Carlos M. Gutierrez, Secretary Technology Administration Phillip J. Bond Under Secretary of Commerce for Technology National Institute of Standards and Technology Hratch G. Semerjian, Jr., Acting Director About ITL For more information about ITL, contact: Information Technology Laboratory National Institute of Standards and Technology 100 Bureau Drive, Stop 8900 Gaithersburg, MD 20899-8900 Telephone: (301) 975-2900 Facsimile: (301) 840-1357 E-mail: [email protected] Website: http://www.itl.nist.gov INFORMATION TECHNOLOGY LABORATORY D IRECTOR’S F OREWORD n today’s complex technology-driven world, the Information Technology Laboratory (ITL) at the National Institute of Standards and Technology has the broad mission of supporting U.S. industry, government, and Iacademia with measurements and standards that enable new computational methods for scientific inquiry, assure IT innovations for maintaining global leadership, and re-engineer complex societal systems and processes through insertion of advanced information technology. Through its efforts, ITL seeks to enhance productivity and public safety, facilitate trade, and improve the Dr. Shashi Phoha, quality of life. ITL achieves these goals in areas of Director, Information national priority by drawing on its core capabilities in Technology Laboratory cyber security, software quality assurance, advanced networking, information access, mathematical and computational sciences, and statistical engineering. utilizing existing and emerging IT to meet national Information technology is the acknowledged engine for priorities that reflect the country’s broad based social, national and regional economic growth. -
Laboratory Supplies and Equipment
Laboratory Supplies and Equipment Beakers: 9 - 12 • Beakers with Handles • Printed Square Ratio Beakers • Griffin Style Molded Beakers • Tapered PP, PMP & PTFE Beakers • Heatable PTFE Beakers Bottles: 17 - 32 • Plastic Laboratory Bottles • Rectangular & Square Bottles Heatable PTFE Beakers Page 12 • Tamper Evident Plastic Bottles • Concertina Collapsible Bottle • Plastic Dispensing Bottles NEW Straight-Side Containers • Plastic Wash Bottles PETE with White PP Closures • PTFE Bottle Pourers Page 39 Containers: 38 - 42 • Screw Cap Plastic Jars & Containers • Snap Cap Plastic Jars & Containers • Hinged Lid Plastic Containers • Dispensing Plastic Containers • Graduated Plastic Containers • Disposable Plastic Containers Cylinders: 45 - 48 • Clear Plastic Cylinder, PMP • Translucent Plastic Cylinder, PP • Short Form Plastic Cylinder, PP • Four Liter Plastic Cylinder, PP NEW Polycarbonate Graduated Bottles with PP Closures Page 21 • Certified Plastic Cylinder, PMP • Hydrometer Jar, PP • Conical Shape Plastic Cylinder, PP Disposal Boxes: 54 - 55 • Bio-bin Waste Disposal Containers • Glass Disposal Boxes • Burn-upTM Bins • Plastic Recycling Boxes • Non-Hazardous Disposal Boxes Printed Cylinders Page 47 Drying Racks: 55 - 56 • Kartell Plastic Drying Rack, High Impact PS • Dynalon Mega-Peg Plastic Drying Rack • Azlon Epoxy Coated Drying Rack • Plastic Draining Baskets • Custom Size Drying Racks Available Burn-upTM Bins Page 54 Dynalon® Labware Table of Contents and Introduction ® Dynalon Labware, a leading wholesaler of plastic lab supplies throughout