Information Technology Laboratory Technical Accomplishments
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Use of Laboratory Equipement
USE OF LABORATORY EQUIPEMENT C. Laboratory Thermometers Most thermometers are based upon the principle that liquids expand when heated. Most common thermometers use mercury or colored alcohol as the liquid. These thermometers are constructed as that a uniform-diameter capillary tube surmounts a liquid reservoir. To calibrate a thermometer, one defines two reference points, normally the freezing point of water (0°C, 32°F) and the boiling point of water (100°C, 212°F) at 1 tam of pressure (1 tam = 760 mm Hg). Once these points are marked on the capillary, its length is then subdivided into uniform divisions called degrees. There are 100° between these two points on the Celsius (°C, or centigrade) scale and 180° between those two points on the Fahrenheit (°F) scale. °F = 1.8 °C + 32 The Thermometer and Its Calibration This section describes the proper technique for checking the accuracy of your thermometer. These measurements will show how measured temperatures (read from thermometer) compare with true temperatures (the boiling and freezing points of water). The freezing point of water is 0°C; the boiling point depends upon atmospheric pressure but at sea level it is 100°C. Option 1: Place approximately 50 mL of ice in a 250-mL beaker and cover the ice with distilled water. Allow about 15 min for the mixture to come to equilibrium and then measure and record the temperature of the mixture. Theoretically, this temperature is 0°C. Option 2: Set up a 250-mL beaker on a wire gauze and iron ring. Fill the beaker about half full with distilled water. -
Laboratory Glassware N Edition No
Laboratory Glassware n Edition No. 2 n Index Introduction 3 Ground joint glassware 13 Volumetric glassware 53 General laboratory glassware 65 Alphabetical index 76 Índice alfabético 77 Index Reference index 78 [email protected] Scharlau has been in the scientific glassware business for over 15 years Until now Scharlab S.L. had limited its sales to the Spanish market. However, now, coinciding with the inauguration of the new workshop next to our warehouse in Sentmenat, we are ready to export our scientific glassware to other countries. Standard and made to order Products for which there is regular demand are produced in larger Scharlau glassware quantities and then stocked for almost immediate supply. Other products are either manufactured directly from glass tubing or are constructed from a number of semi-finished products. Quality Even today, scientific glassblowing remains a highly skilled hand craft and the quality of glassware depends on the skill of each blower. Careful selection of the raw glass ensures that our final products are free from imperfections such as air lines, scratches and stones. You will be able to judge for yourself the workmanship of our glassware products. Safety All our glassware is annealed and made stress free to avoid breakage. Fax: +34 93 715 67 25 Scharlab The Lab Sourcing Group 3 www.scharlab.com Glassware Scharlau glassware is made from borosilicate glass that meets the specifications of the following standards: BS ISO 3585, DIN 12217 Type 3.3 Borosilicate glass ASTM E-438 Type 1 Class A Borosilicate glass US Pharmacopoeia Type 1 Borosilicate glass European Pharmacopoeia Type 1 Glass The typical chemical composition of our borosilicate glass is as follows: O Si 2 81% B2O3 13% Na2O 4% Al2O3 2% Glass is an inorganic substance that on cooling becomes rigid without crystallising and therefore it has no melting point as such. -
PML Brochure
PHYSICAL MEASUREMENT LABORATORY Gauging nature on all scales NIST.GOV/PML PHYSICAL MEASUREMENT LABORATORY (PML) The Physical Measurement Laboratory (PML), a major frequency, electricity, temperature, humidity, pressure operating unit of the National Institute of Standards and vacuum, liquid and gas flow, and electromag- and Technology (NIST), sets the definitive U.S. standards netic, optical, acoustic, and ionizing radiation. PML for nearly every kind of measurement in modern life, collaborates directly with industry, universities, profes- sometimes across more than 20 orders of magni- sional and standards-setting organizations, and other tude. PML is a world leader in the science of physical agencies of government to ensure accuracy and to measurement, devising procedures and tools that make solve problems. It also supports research in many continual progress possible. Exact measurements are fields of urgent national importance, such as manu- absolutely essential to industry, medicine, the research facturing, energy, health, law enforcement and community, and government. All of them depend on homeland security, communications, military defense, PML to develop, maintain, and disseminate the official electronics, the environment, lighting and display, standards for a wide range of quantities, including radiation, remote sensing, space exploration, length, mass, force and shock, acceleration, time and and transportation. IMPACTS ❱ Provides 700 kinds of calibration services ❱ Numerous special testing services NIST.GOV/PML | 2 NIST.GOV/PML -
Chemostat Culture for Yeast Experimental Evolution
Downloaded from http://cshprotocols.cshlp.org/ at Cold Spring Harbor Laboratory Library on August 9, 2017 - Published by Cold Spring Harbor Laboratory Press Protocol Chemostat Culture for Yeast Experimental Evolution Celia Payen and Maitreya J. Dunham1 Department of Genome Sciences, University of Washington, Seattle, Washington 98195 Experimental evolution is one approach used to address a broad range of questions related to evolution and adaptation to strong selection pressures. Experimental evolution of diverse microbial and viral systems has routinely been used to study new traits and behaviors and also to dissect mechanisms of rapid evolution. This protocol describes the practical aspects of experimental evolution with yeast grown in chemostats, including the setup of the experiment and sampling methods as well as best laboratory and record-keeping practices. MATERIALS It is essential that you consult the appropriate Material Safety Data Sheets and your institution’s Environmental Health and Safety Office for proper handling of equipment and hazardous material used in this protocol. Reagents Defined minimal medium appropriate for the experiment For examples, see Protocol: Assembly of a Mini-Chemostat Array (Miller et al. 2015). Ethanol (95%) Glycerol (20% and 50%; sterile) Yeast strain of interest Equipment Agar plates (appropriate for chosen strain) Chemostat array Assemble the apparatus as described in Miller et al. (2013) and Protocol: Assembly of a Mini-Chemostat Array (Miller et al. 2015). Cryo deep-freeze labels Cryogenic vials Culture tubes Cytometer (BD Accuri C6) Glass beads, 4 mm (sterile; for plating yeast cells) Glass cylinder Kimwipes 1Correspondence: [email protected] © 2017 Cold Spring Harbor Laboratory Press Cite this protocol as Cold Spring Harb Protoc; doi:10.1101/pdb.prot089011 559 Downloaded from http://cshprotocols.cshlp.org/ at Cold Spring Harbor Laboratory Library on August 9, 2017 - Published by Cold Spring Harbor Laboratory Press C. -
BROOKFIELD DIAL READING VISCOMETER with Electronic Drive
BROOKFIELD DIAL READING VISCOMETER with Electronic Drive Operating Instructions Manual No. M00-151-I0614 SPECIALISTS IN THE MEASUREMENT AND CONTROL OF VISCOSITY with offices in : Boston • Chicago • London • Stuttgart • Guangzhou BROOKFIELD ENGINEERING LABORATORIES, INC. 11 Commerce Boulevard, Middleboro, MA 02346 USA TEL 508-946-6200 or 800-628-8139 (USA excluding MA) FAX 508-946-6262 INTERNET http://www.brookfieldengineering.com TABLE OF CONTENTS I. INTRODUCTION .....................................................................................5 I.1 Components .......................................................................................................5 I.2 Utilities ................................................................................................................6 I.3 Specifications .....................................................................................................6 I.4 Set-Up ................................................................................................................7 I.5 IQ, OQ, PQ .........................................................................................................7 I.6 Safety Symbols and Precautions .......................................................................8 I.7 Cleaning .............................................................................................................8 II. GETTING STARTED ..............................................................................9 II.1 Operation ...........................................................................................................9 -
ELISA Plate Reader
applications guide to microplate systems applications guide to microplate systems GETTING THE MOST FROM YOUR MOLECULAR DEVICES MICROPLATE SYSTEMS SALES OFFICES United States Molecular Devices Corp. Tel. 800-635-5577 Fax 408-747-3601 United Kingdom Molecular Devices Ltd. Tel. +44-118-944-8000 Fax +44-118-944-8001 Germany Molecular Devices GMBH Tel. +49-89-9620-2340 Fax +49-89-9620-2345 Japan Nihon Molecular Devices Tel. +06-6399-8211 Fax +06-6399-8212 www.moleculardevices.com ©2002 Molecular Devices Corporation. Printed in U.S.A. #0120-1293A SpectraMax, SoftMax Pro, Vmax and Emax are registered trademarks and VersaMax, Lmax, CatchPoint and Stoplight Red are trademarks of Molecular Devices Corporation. All other trademarks are proprty of their respective companies. complete solutions for signal transduction assays AN EXAMPLE USING THE CATCHPOINT CYCLIC-AMP FLUORESCENT ASSAY KIT AND THE GEMINI XS MICROPLATE READER The Molecular Devices family of products typical applications for Molecular Devices microplate readers offers complete solutions for your signal transduction assays. Our integrated systems γ α β s include readers, washers, software and reagents. GDP αs AC absorbance fluorescence luminescence GTP PRINCIPLE OF CATCHPOINT CYCLIC-AMP ASSAY readers readers readers > Cell lysate is incubated with anti-cAMP assay type SpectraMax® SpectraMax® SpectraMax® VersaMax™ VMax® EMax® Gemini XS LMax™ ATP Plus384 190 340PC384 antibody and cAMP-HRP conjugate ELISA/IMMUNOASSAYS > nucleus Single addition step PROTEIN QUANTITATION cAMP > λEX 530 nm/λEM 590 nm, λCO 570 nm UV (280) Bradford, BCA, Lowry For more information on CatchPoint™ assay NanoOrange™, CBQCA kits, including the complete procedure for this NUCLEIC ACID QUANTITATION assay (MaxLine Application Note #46), visit UV (260) our web site at www.moleculardevices.com. -
An Introduction to the SAS System
An Introduction to the SAS System Dileep K. Panda Directorate of Water Management Bhubaneswar-751023 [email protected] Introduction The SAS – Statistical Analysis System (erstwhile expansion of SAS) - is the one of the most widely used Statistical Software package by the academic circles and Industry. The SAS software was developed in late 1960s at North Carolina State University and in 1976 SAS Institute was formed. The SAS system is a collection of products, available from the SAS Institute in North Carolina. SAS software is a combination of a statistical package, a data – base management system, and a high level programming language. The SAS is an integrated system of software solutions that performs the following tasks: Data entry, retrieval, and management Report writing and graphics design Statistical and mathematical analysis Business forecasting and decision support Operations research and project management Applications development At the core of the SAS System is the Base SAS software. The Base SAS software includes a fourth-generation programming language and ready-to-use programs called procedures. These integrated procedures handle data manipulation, information storage and retrieval, statistical analysis, and report writing. Additional components offer capabilities for data entry, retrieval, and management; report writing and graphics; statistical and mathematical analysis; business planning, forecasting, and decision support; operations research and project management; quality improvement; and applications development. In general, the Base SAS software has the following capabilities A data management facility A programming language Data analysis and reporting utilities Learning to use Base SAS enables you to work with these features of SAS. It also prepares you to learn other SAS products, because all SAS products follow the same basic rules. -
The-Pathologists-Microscope.Pdf
The Pathologist’s Microscope The Pathologist’s Microscope Rudolf Virchow, the father of Pathology, had available to him wonderful microscopes during the 1850’s to 1880’s, but the one you have now is far better. Your microscope is the most highly perfected of all scientific instruments. These brief notes on alignment, the objective lens, the condenser, and the eyepieces are what you need to know to get the most out of your microscope and to feel comfortable using it. Figure 1 illustrates the important parts of a generic modern light microscope. Figure 1 - Parts of the Microscope UNC Pathology & Lab Med, MSL, July 2013 1 The Pathologist’s Microscope Alignment August Köhler, in 1870, invented the method for aligning the microscope’s optical system that is still used in all modern microscopes. To get the most from your microscope it should be Köhler aligned. Here is how: 1. Focus a specimen slide at 10X. 2. Open the field iris and the condenser iris. 3. Observe the specimen and close the field iris until its shadow appears on the specimen. 4. Use the condenser focus knob to bring the field iris into focus on the specimen. Try for as sharp an image of the iris as you can get. If you can’t focus the field iris, check the condenser for a flip-in lens and find the configuration that lets you see the field iris. You may also have to move the field iris into the field of view (step 5) if it is grossly misaligned. 5.Center the field iris with the condenser centering screws. -
Microplate Selection and Recommended Practices in High-Throughput Screening and Quantitative Biology
NLM Citation: Auld DSPh.D., Coassin PAB.S., Coussens NPPh.D., et al. Microplate Selection and Recommended Practices in High-throughput Screening and Quantitative Biology. 2020 Jun 1. In: Sittampalam GS, Grossman A, Brimacombe K, et al., editors. Assay Guidance Manual [Internet]. Bethesda (MD): Eli Lilly & Company and the National Center for Advancing Translational Sciences; 2004-. Bookshelf URL: https://www.ncbi.nlm.nih.gov/books/ Microplate Selection and Recommended Practices in High-throughput Screening and Quantitative Biology Douglas S. Auld, Ph.D.,1 Peter A. Coassin, B.S.,2 Nathan P. Coussens, Ph.D.,3 Paul Hensley,4 Carleen Klumpp-Thomas,5 Sam Michael,5 G. Sitta Sittampalam, Ph.D.,5 O. Joseph Trask, B.S.,6 Bridget K. Wagner, Ph.D.,7 Jeffrey R. Weidner, Ph.D.,8 Mary Jo Wildey, Ph.D.,9 and Jayme L. Dahlin, M.D., Ph.D. 7,10 Created: June 1, 2020. Abstract High-throughput screening (HTS) can efficiently assay multiple discrete biological reactions using multi-well microplates. The choice of microplate is a crucial yet often overlooked technical decision in HTS and quantitative biology. This chapter reviews key criteria for microplate selection, including: well number, well volume and shape, microplate color, surface treatments/coatings, and considerations for specialized applications such as high-content screening. This chapter then provides important technical advice for microplate handling including mixing, dispensing, incubation, and centrifugation. Special topics are discussed, including microplate surface properties, plate washing, well-to-well contamination, microplate positional effects, well-to-well and inter-lot variability, and troubleshooting. This information and best practices derived from academic, government, and industry screening centers, as well as microplate vendors, should accelerate assay development and enhance assay quality. -
PLATE READER INTRODUCTION Selecting the Right Plate Reader for Your Lab Can Be Challenging
CHOOSING THE RIGHT PLATE READER INTRODUCTION Selecting the right plate reader for your lab can be challenging. There are multiple factors to consider including: detection type, additional features, support, and price. With numerous options for each variable, it can be confusing to navigate the selection process. Focusing on striking a balance between functionality, optimization, and growth potential requires keen insight and continued vendor support throughout the life cycle of the plate reader. This detailed guide will help you choose the right plate reader for your needs by examining the marketplace and pointing out considerations to keep in mind when making your decision. UNDERSTANDING THE PROCESS A few key questions can help customers navigate toward the best fit for a reader meeting the individual specification requirements for your lab: What detection technology should you look for when selecting a plate reader for your lab? Is this technology ideal for multiple therapeutic areas? How can you get the best performance optimization out of your reader? What support options does your vendor offer in conjunction with the purchase? WHAT DETECTION TECHNOLOGY SHOULD YOU LOOK FOR WHEN SELECTING A PLATE READER FOR YOUR LAB? Whether a lab performs development, high-throughput screening, or a more specialized area of focus, a reader should offer, at minimum, the requirements needed for each individual laboratory. For example, a growing lab focused on performing reporter gene assays requires a plate reader that delivers on the importance of sensitivity while offering the potential for future upgrades or additional features as the lab matures over time. Vendors with reader option focused on providing sensitivity, speed, and multiplex capabilities can meet the sensitivity needs of reporter gene assay performance while offering additional reading technologies for customization and accommodating the fluctuation of industry trends in support of long-term growth. -
Precision Lab-Line Water Baths
Thermo Scientific Precision and Lab-Line Water Baths outstanding performance and reliability for laboratory applications Thermo Scientific Precision and Lab-Line Water Baths value, performance and reliability Thermo Scientific™ Precision™ and Thermo Scientific™ Lab-Line™ water baths provide outstanding performance and reliability for your laboratory applications. Available in a variety of models and capacities, our water baths can be equipped with a broad range of accessories to support your specific application requirements. Applications Precision & Precision Circulating Precision Shaking Lab-Line General Purpose Models 260, Coliform Models Dubnoff and water baths 265, 270 25 and 50 Shallow Form Bacteriological Examinations • • • • • Coagulation Tests • • • Coliform Determinations • • • Copper Strip Corrosion Tests • • • Crude Oil Studies • • Cytochemistry • • • Dialysis • Demulsibility Studies • • Enzyme Studies • • Electrophoresis Gel Destaining • Environmental Studies • • • • • Food Processing QC • • • • Genetic Studies • • • • Hormone Studies • • • • Immunological Research • • • • Incubation for Microbiological Assays • • • • Incubation for Microcentrifugation Tubes • • Melting Agar • Metallurgical Analysis • • • • Molecular Biology • • • • Protein Analysis • • Radioactive Isotope Uptake Studies • • • • Radiochemistry • • • Serological Research • • • • Thawing • • • • Thawing Blood • • • • Thawing Cryopreservation Vials • Tissue Culture Research • • • • Virology Research • • • • Warming Reagents • • Water Quality Research • • • • -
Paquetes Estadísticos Con Licencia Libre (I) Free Statistical
2013, Vol. 18 No 2, pp. 12-33 http://www.uni oviedo.es/reunido /index.php/Rema Paquetes estadísticos con licencia libre (I) Free statistical software (I) Carlos Carleos Artime y Norberto Corral Blanco Departamento de Estadística e I. O. y D.M.. Universidad de Oviedo RESUMEN El presente artículo, el primero de una serie de trabajos, presenta un panorama de los principales paquetes estadísticos disponibles en la actualidad con licencia libre, entendiendo este concepto a partir de los grandes proyectos informáticos así autodefinidos que han cristalizado en los últimos treinta años. En esta primera entrega se presta atención fundamentalmente al programa R. Palabras clave : paquetes estadísticos, software libre, R. ABSTRACT This article, the first in a series of works, presents an overview of the major statistical packages available today with free license, understanding this concept from the large computer and self-defined projects that have crystallized in the last thirty years. In this first paper we pay attention mainly to the program R. Keywords : statistical software, free software, R. Contacto: Norberto Corral Blanco Email: [email protected] . 12 Revista Electrónica de Metodología Aplicada (2013), Vol. 18 No 2, pp. 12-33 1.- Introducción La palabra “libre” es usada, y no siempre de manera adecuada, en múltiples campos de conocimiento así que la informática no iba a ser una excepción. La palabra es especialmente problemática porque su traducción al inglés, “free”, es aun más polisémica, e incluye el concepto “gratis”. En 1985 se fundó la Free Software Foundation (FSF) para defender una definición rigurosa del sófguar libre. La propia palabra “sófguar” (del inglés “software”, por oposición a “hardware”, quincalla, aplicado al soporte físico de la informática) es en sí misma problemática.