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Use of Laboratory Equipement
USE OF LABORATORY EQUIPEMENT C. Laboratory Thermometers Most thermometers are based upon the principle that liquids expand when heated. Most common thermometers use mercury or colored alcohol as the liquid. These thermometers are constructed as that a uniform-diameter capillary tube surmounts a liquid reservoir. To calibrate a thermometer, one defines two reference points, normally the freezing point of water (0°C, 32°F) and the boiling point of water (100°C, 212°F) at 1 tam of pressure (1 tam = 760 mm Hg). Once these points are marked on the capillary, its length is then subdivided into uniform divisions called degrees. There are 100° between these two points on the Celsius (°C, or centigrade) scale and 180° between those two points on the Fahrenheit (°F) scale. °F = 1.8 °C + 32 The Thermometer and Its Calibration This section describes the proper technique for checking the accuracy of your thermometer. These measurements will show how measured temperatures (read from thermometer) compare with true temperatures (the boiling and freezing points of water). The freezing point of water is 0°C; the boiling point depends upon atmospheric pressure but at sea level it is 100°C. Option 1: Place approximately 50 mL of ice in a 250-mL beaker and cover the ice with distilled water. Allow about 15 min for the mixture to come to equilibrium and then measure and record the temperature of the mixture. Theoretically, this temperature is 0°C. Option 2: Set up a 250-mL beaker on a wire gauze and iron ring. Fill the beaker about half full with distilled water. -
Oristm Cell Migration Assay – Tricoated
TM Oris Cell Migration Assay – TriCoated Product No.: CMATR1 & CMATR5 96-well, 2-D Assay for Investigating Cell Migration of Adherent Cell Lines on Three Coated Surfaces PROTOCOL & INSTRUCTIONS Table of Contents I. INTRODUCTION .................................................................................................. 2 II. ORISTM PLATE DIMENSIONS .............................................................................. 3 III. MATERIALS PROVIDED ...................................................................................... 3 IV. MATERIALS REQUIRED ...................................................................................... 3 V. CELL MIGRATION ASSAY – TRICOATED PROTOCOL .................................... 4 VI. DATA ACQUISITION ............................................................................................ 6 VII. ORDERING INFORMATION ................................................................................ 7 VIII. TERMS & CONDITIONS ...................................................................................... 7 Appendix I: Determining Optimal Cell Seeding Concentration ............................. 8 Appendix II: Determining Optimal Fluorescence Microplate Reader Settings ...... 8 Platypus Technologies, LLC 5520 Nobel Drive, Suite 100, Madison, WI 53711 Toll Free: 866.3296.4455 Phone: 608.237.1270 Fax: 608.237.1271 www.platypustech.com SP0034.06 Oris™ CELL MIGRATION ASSAY – TRICOATED I. INTRODUCTION The Oris™ Cell Migration Assay – TriCoated is a reproducible, sensitive, and flexible -
Laboratory Glassware N Edition No
Laboratory Glassware n Edition No. 2 n Index Introduction 3 Ground joint glassware 13 Volumetric glassware 53 General laboratory glassware 65 Alphabetical index 76 Índice alfabético 77 Index Reference index 78 [email protected] Scharlau has been in the scientific glassware business for over 15 years Until now Scharlab S.L. had limited its sales to the Spanish market. However, now, coinciding with the inauguration of the new workshop next to our warehouse in Sentmenat, we are ready to export our scientific glassware to other countries. Standard and made to order Products for which there is regular demand are produced in larger Scharlau glassware quantities and then stocked for almost immediate supply. Other products are either manufactured directly from glass tubing or are constructed from a number of semi-finished products. Quality Even today, scientific glassblowing remains a highly skilled hand craft and the quality of glassware depends on the skill of each blower. Careful selection of the raw glass ensures that our final products are free from imperfections such as air lines, scratches and stones. You will be able to judge for yourself the workmanship of our glassware products. Safety All our glassware is annealed and made stress free to avoid breakage. Fax: +34 93 715 67 25 Scharlab The Lab Sourcing Group 3 www.scharlab.com Glassware Scharlau glassware is made from borosilicate glass that meets the specifications of the following standards: BS ISO 3585, DIN 12217 Type 3.3 Borosilicate glass ASTM E-438 Type 1 Class A Borosilicate glass US Pharmacopoeia Type 1 Borosilicate glass European Pharmacopoeia Type 1 Glass The typical chemical composition of our borosilicate glass is as follows: O Si 2 81% B2O3 13% Na2O 4% Al2O3 2% Glass is an inorganic substance that on cooling becomes rigid without crystallising and therefore it has no melting point as such. -
PML Brochure
PHYSICAL MEASUREMENT LABORATORY Gauging nature on all scales NIST.GOV/PML PHYSICAL MEASUREMENT LABORATORY (PML) The Physical Measurement Laboratory (PML), a major frequency, electricity, temperature, humidity, pressure operating unit of the National Institute of Standards and vacuum, liquid and gas flow, and electromag- and Technology (NIST), sets the definitive U.S. standards netic, optical, acoustic, and ionizing radiation. PML for nearly every kind of measurement in modern life, collaborates directly with industry, universities, profes- sometimes across more than 20 orders of magni- sional and standards-setting organizations, and other tude. PML is a world leader in the science of physical agencies of government to ensure accuracy and to measurement, devising procedures and tools that make solve problems. It also supports research in many continual progress possible. Exact measurements are fields of urgent national importance, such as manu- absolutely essential to industry, medicine, the research facturing, energy, health, law enforcement and community, and government. All of them depend on homeland security, communications, military defense, PML to develop, maintain, and disseminate the official electronics, the environment, lighting and display, standards for a wide range of quantities, including radiation, remote sensing, space exploration, length, mass, force and shock, acceleration, time and and transportation. IMPACTS ❱ Provides 700 kinds of calibration services ❱ Numerous special testing services NIST.GOV/PML | 2 NIST.GOV/PML -
Chemostat Culture for Yeast Experimental Evolution
Downloaded from http://cshprotocols.cshlp.org/ at Cold Spring Harbor Laboratory Library on August 9, 2017 - Published by Cold Spring Harbor Laboratory Press Protocol Chemostat Culture for Yeast Experimental Evolution Celia Payen and Maitreya J. Dunham1 Department of Genome Sciences, University of Washington, Seattle, Washington 98195 Experimental evolution is one approach used to address a broad range of questions related to evolution and adaptation to strong selection pressures. Experimental evolution of diverse microbial and viral systems has routinely been used to study new traits and behaviors and also to dissect mechanisms of rapid evolution. This protocol describes the practical aspects of experimental evolution with yeast grown in chemostats, including the setup of the experiment and sampling methods as well as best laboratory and record-keeping practices. MATERIALS It is essential that you consult the appropriate Material Safety Data Sheets and your institution’s Environmental Health and Safety Office for proper handling of equipment and hazardous material used in this protocol. Reagents Defined minimal medium appropriate for the experiment For examples, see Protocol: Assembly of a Mini-Chemostat Array (Miller et al. 2015). Ethanol (95%) Glycerol (20% and 50%; sterile) Yeast strain of interest Equipment Agar plates (appropriate for chosen strain) Chemostat array Assemble the apparatus as described in Miller et al. (2013) and Protocol: Assembly of a Mini-Chemostat Array (Miller et al. 2015). Cryo deep-freeze labels Cryogenic vials Culture tubes Cytometer (BD Accuri C6) Glass beads, 4 mm (sterile; for plating yeast cells) Glass cylinder Kimwipes 1Correspondence: [email protected] © 2017 Cold Spring Harbor Laboratory Press Cite this protocol as Cold Spring Harb Protoc; doi:10.1101/pdb.prot089011 559 Downloaded from http://cshprotocols.cshlp.org/ at Cold Spring Harbor Laboratory Library on August 9, 2017 - Published by Cold Spring Harbor Laboratory Press C. -
White Paper Absorbance Or Fluorescence: Which Is the Best
WHITE PAPER No. 40 Absorbance or Fluorescence: Which Is the Best Way to Quantify Nucleic Acids? Natascha Weiß1, Martin Armbrecht1 ¹Eppendorf AG, Hamburg, Germany Executive Summary When performing molecular experiments on nucleic acids, it is a basic requirement to determine the concentration as well as the quality of the sample. The standard method which serves this purpose is UV-Vis spectrophotometry – but is it always the right choice? In the present White Paper, the advantages and disadvantages of this technique will be described and it will be compared with nucleic acid quantification via fluorescence. In this context, it will be discussed which situations warrant the use of which one of the two methods. The decision will generally depend on the condition of the sample as well as on the requirements of downstream applications. Since the advantages of both methods complement each other well, it is most practical to have the ability to perform both methods on a single instrument in a flexible manner. Introduction Experiments involving nucleic acids are a mainstay in any evaluated by measuring the sample at additional wave molecular laboratory. DNA and RNA are isolated from micro- lengths (230 nm, 280 nm) and calculating the purity ratios, organisms and from cells of higher order organisms in order i.e. the ratios of the values obtained at 260/230 nm and at to be employed in a broad variety of processing steps and 260/280 nm, respectively. In this way, it will be evident analyses. It is crucial for any type of downstream applica- whether cellular debris or remainders of reagent used during tion that a defined amount of nucleic acid is used and that purification such as proteins, sugar molecules, certain salts the sample is free from contaminations that may impact the or phenols, are present in the solution, as these will generate experiments. -
Increasing the Reproducibility of Serial Dilutions
Increasing the Reproducibility of Serial Dilutions Michael Beier* Abstract Pipetting serial dilutions reproducibly can be difficult because it is a time consuming and consequently error prone methodology, especially in 384 well plates. Based upon the assumption that your pipettes are properly maintained and calibrated, the human influence has the largest impact on pipetting results. Observing good pipetting practice helps immensely to improve reproducibility and accuracy. In this article we focus on a selection of good pipetting techniques, which if maintained offer the best chance of increasing and maintaining the reproducibility of your serial dilutions. Mixing is a key component of dilution protocols and in this article we additionally discuss different mixing parameters to help you achieve a homogenous sample. To maximize consistency of dilution assays, automating the process offers many benefits. For ensuring highly reproducible serial dilutions we outline what features an automated pipetting system should have and introduce the VIAFLO ASSIST – a product purpose designed for this task. Introduction to good pipetting practice Good pipetting practice describes guidelines covering the basic techniques to ensure that the pipettes are used in an optimal and reproducible manner. Before preparing your assay, you should perform a pre-wet of your pipette tips. Do this by aspirating and dispensing the full volume 2-3 times. This procedure not only equilibrates temperature differences between liquid and tip but also humidifies the dead air space inside the pipette and tip. Neglecting to perform a pre-wet can result in a smaller delivery volume in the first few dispenses, thereby introducing errors into your subsequent dilution steps. -
On Food, Spectrophotometry, and Measurement Data Processing (Keynote Lecture)
12th IMEKO TC1 & TC7 Joint Symposium on Man Science & Measurement September, 3–5, 2008, Annecy, France ON FOOD, SPECTROPHOTOMETRY, AND MEASUREMENT DATA PROCESSING (KEYNOTE LECTURE) Roman Z. Morawski Warsaw University of Technology, Faculty of Electronics and Information Technology, Institute of Radioelectronics Warsaw, Poland [email protected] Abstract: Spectrophotometry is getting more and more Despite philosophical abnegation of food issues, the often the method of choice not only in laboratory analysis of practice of food preparation and refinement has flourished (bio)chemical substances, but also in the off-laboratory for centuries, and inspired research-and-invention-oriented identification and testing of physical properties of various minds. Today, we may speak about a fully developed products, in particular – of various organic mixtures discipline of science and technology. Enough to say that the including food products and ingredients. Specialized Institute of Food Technologists – the largest international, spectrophotometers, called spectrophotometric analyzers are non-profit professional organization involved in the designed for such applications. This keynote lecture is on advancement of food science and technology – is the state of the art and developmental trends in the domain encompassing 23 000 members worldwide. Its Committee of spectrophotometric analyzers of food with particular on Higher Education provided the following definition of emphasis on wine analyzers. The following issues are food science: "Food science is the discipline in which the covered: philosophical and methodological background of engineering, biological, and physical sciences are used to food analysis, physical and metrological principles of study the nature of foods, the causes of deterioration, the spectrophotometry, the role of measurement data processing principles underlying food processing, and the improvement in spectrophotometry, food analyzers on the market and of foods for the consuming public" [1]. -
Evos Cell Imaging Analysis Systems
Imaging, labeling, and detection solutions Microscopy | High-content analysis | Cell counting | Plate reading Minimizing the complexities of cellular analysis Our cellular analysis product portfolio combines the strengths of Invitrogen™ fluorescent reagents and a complete line of versatile analysis instrumentation. Select from a line of heavily peer-referenced platforms to make the discoveries that catalyze advances toward your research goals of tomorrow. Invitrogen™ EVOS™ imaging systems Our comprehensive imaging portfolio includes: • Cell imaging systems • Automated cell counting systems • High-content analysis systems Invitrogen™ Countess™ cell counters • Cell imaging reagents • Microplate readers All of our analysis systems are designed to work together— Thermo Scientific™ CellInsight™ from the initial cell culture check to more complex analyses. high-content analysis systems Discover more about your samples with automated cell counting, long-term live-cell imaging, automated multiwell plate scanning, and phenotypic screening. Thermo Scientific™ Varioskan™ LUX multimode plate reader Contents Microscopy Compact and portable imaging systems 4 EVOS imaging systems at a glance 5 EVOS M7000 Imaging System 6 Live-cell imaging with the Onstage Incubator 8 Image analysis with Celleste software 10 EVOS M5000 Imaging System 12 EVOS FLoid Imaging Station 14 EVOS XL Core Imaging System 15 EVOS vessel holders and stage plates 16 The power of LED illumination 17 EVOS objectives 18 Fluorophore selection guide 20 High-content analysis CellInsight high-content analysis platforms 22 CellInsight CX7 LZR High-Content Analysis Platform 24 CellInsight CX5 High-Content Screening Platform 25 HCS Studio Cell Analysis Software 26 Store Image and Database Management Software 27 Cell counting Countess II Automated Cell Counters 28 Plate reading Microplate readers 30 Microscopy Compact and portable imaging systems Now you can have an easy-to-use cell imaging platform From intimate hands-on demonstrations to presentations of where you want it and when you want it. -
BROOKFIELD DIAL READING VISCOMETER with Electronic Drive
BROOKFIELD DIAL READING VISCOMETER with Electronic Drive Operating Instructions Manual No. M00-151-I0614 SPECIALISTS IN THE MEASUREMENT AND CONTROL OF VISCOSITY with offices in : Boston • Chicago • London • Stuttgart • Guangzhou BROOKFIELD ENGINEERING LABORATORIES, INC. 11 Commerce Boulevard, Middleboro, MA 02346 USA TEL 508-946-6200 or 800-628-8139 (USA excluding MA) FAX 508-946-6262 INTERNET http://www.brookfieldengineering.com TABLE OF CONTENTS I. INTRODUCTION .....................................................................................5 I.1 Components .......................................................................................................5 I.2 Utilities ................................................................................................................6 I.3 Specifications .....................................................................................................6 I.4 Set-Up ................................................................................................................7 I.5 IQ, OQ, PQ .........................................................................................................7 I.6 Safety Symbols and Precautions .......................................................................8 I.7 Cleaning .............................................................................................................8 II. GETTING STARTED ..............................................................................9 II.1 Operation ...........................................................................................................9 -
Dynapro® Plate Reader™ II User's Guide
DynaPro® Plate Reader™ II User’s Guide M3101 Rev C Copyright © 2014, Wyatt Technology Corporation WYATT TECHNOLOGY Corp., makes no warranties, either express or implied, regarding this instrument, computer software package, its merchantability or its fitness for any particular purpose. The software is provided “as is,” without warranty of any kind. Furthermore, Wyatt Technology does not warrant, guarantee, or make any representations regarding the use, or the results of the use, of the software or written materials in terms of correctness, accuracy, reliability, currentness, or otherwise. The exclusion of implied warranties is not permitted by some states, so the above exclusion may not apply to you. All rights reserved. No part of this publication may be reproduced, stored in a retrieval system, or transmitted, in any form by any means, electronic, mechanical, photocopying, recording, or otherwise, without the prior written permission of Wyatt Technology Corporation. ® DynaPro, DYNAMICS, Wyatt Technology, and its logo are registered trademarks of the Wyatt Technology Corporation. ™ Plate Reader and Protein Solutions are trademarks of Wyatt Technology Corporation. A variety of U.S. and foreign patents have been issued and/or are pending on various aspects of the apparatus and methodology implemented by this instrumentation. Table of Contents Chapter 1: Introduction Overview ............................................................................................................................6 The Instrument ............................................................................................................6 -
Information Technology Laboratory Technical Accomplishments
CONTENTS Director’s Foreword 1 ITL at a Glance 4 ITL Research Blueprint 6 Accomplishments of our Research Program 7 Foundation Research Areas 8 Selected Cross-Cutting Themes 26 Industry and International Interactions 36 Publications 44 NISTIR 7169 Conferences 47 February 2005 Staff Recognition 50 U.S. DEPARTMENT OF COMMERCE Carlos M. Gutierrez, Secretary Technology Administration Phillip J. Bond Under Secretary of Commerce for Technology National Institute of Standards and Technology Hratch G. Semerjian, Jr., Acting Director About ITL For more information about ITL, contact: Information Technology Laboratory National Institute of Standards and Technology 100 Bureau Drive, Stop 8900 Gaithersburg, MD 20899-8900 Telephone: (301) 975-2900 Facsimile: (301) 840-1357 E-mail: [email protected] Website: http://www.itl.nist.gov INFORMATION TECHNOLOGY LABORATORY D IRECTOR’S F OREWORD n today’s complex technology-driven world, the Information Technology Laboratory (ITL) at the National Institute of Standards and Technology has the broad mission of supporting U.S. industry, government, and Iacademia with measurements and standards that enable new computational methods for scientific inquiry, assure IT innovations for maintaining global leadership, and re-engineer complex societal systems and processes through insertion of advanced information technology. Through its efforts, ITL seeks to enhance productivity and public safety, facilitate trade, and improve the Dr. Shashi Phoha, quality of life. ITL achieves these goals in areas of Director, Information national priority by drawing on its core capabilities in Technology Laboratory cyber security, software quality assurance, advanced networking, information access, mathematical and computational sciences, and statistical engineering. utilizing existing and emerging IT to meet national Information technology is the acknowledged engine for priorities that reflect the country’s broad based social, national and regional economic growth.