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Modulus™ II Microplate Multimode Reader

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Modulus™ II Microplate Multimode Reader Modulus™ II Microplate Multimode Reader Operating Manual Part Number 998-9375 Rev E Turner BioSystems, Inc. and its suppliers own the written and other materials in the Modulus II Microplate Reader operating manual. The written materials are copyrighted and protected by the copyright laws of the United States of America and, throughout the world, by the copyright laws of each jurisdiction according to pertinent treaties and conventions. No part of this manual may be reproduced manually, electronically, or in any other way without the written permission of Turner BioSystems, Inc. Modulus™ is a trademark of Turner BioSystems, Inc. All of the trademarks, service marks, and logos used in these materials are the property of Turner BioSystems, Inc. or their respective owners. All goods and services are sold subject to the terms and conditions of sale of the company within the Turner BioSystems, Inc. group that supplies them. A copy of these terms and conditions is available on request. NOTICE TO PURCHASER: The Modulus™ II Microplate Reader is for research purposes only. It is not intended or approved for diagnosis of disease in humans or animals. © Turner BioSystems, Inc. 2008 - All rights reserved. Made in the USA 2 Table of Contents 1 Instrument ............................................................................................................................... 7 1.1 Getting Started ................................................................................................................. 7 1.1.1 Description ............................................................................................................ 7 1.1.2 Precautions ........................................................................................................... 7 1.1.3 Unpacking and Inspection ..................................................................................... 8 1.1.4 Instrument Set Up ................................................................................................. 8 1.1.5 Instrument Overview ............................................................................................. 9 1.2 Touch Screen Basics ....................................................................................................... 10 1.2.1 Buttons and Icons on the INSTRUMENT CONTROL Screen............................... 11 1.2.2 Buttons on the READ Screen ................................................................................ 12 1.2.3 Buttons on the RESULTS Screen ......................................................................... 13 1.2.4 Buttons on the TOOLS Screen ............................................................................. 13 1.2.5 Terminology used in Parameter Settings .............................................................. 14 1.3 HOME Screen .................................................................................................................... 15 1.4 Protocol Management ...................................................................................................... 15 1.4.1 Defining a New Protocol through Instrument Control ............................................ 16 1.4.2 Modifying a saved protocol ................................................................................... 17 1.4.3 To save the Modified Protocol ............................................................................... 17 1.5 Using the Protocol Composer to Set Up Multiple Read Formats ................................ 18 1.5.1 Defining a Multiple Parameter Option Protocol ..................................................... 18 1.6 Plate Format Selection ..................................................................................................... 19 1.6.1 Plate Format Selection .......................................................................................... 20 1.6.2 Well Selection ........................................................................................................ 20 1.6.3 Selecting Reference Wells .................................................................................... 21 1.6.4 Changing the Plate Adapter Orientation ............................................................... 21 1.6.5 Changing the Optical Crosstalk Mask for 384-well option .................................... 22 1.6.6 Well Scan mode for 6 to 48-well formats .............................................................. 23 1.6.7 Viewing multiple read results for 6 to 48 well plate formats .................................. 23 1.6.8 Accessing the Interior of the Instrument................................................................ 24 1.7 Viewing the Data in the RESULTS Screen ..................................................................... 24 1.8 Transferring Data ............................................................................................................. 25 1.9 Repeat Runs ..................................................................................................................... 26 1.9.1 Repeat Runs Parameters ...................................................................................... 26 1.9.2 Viewing Repeat Runs Data on the RESULTS Screen .......................................... 26 2 Luminescence ............................................................................................................................ 27 2.1 General Information ......................................................................................................... 27 2.2 Microplate Sample Plate Cover ....................................................................................... 27 2.3 PMT Activation ................................................................................................................. 27 2.4 Starting a Run ................................................................................................................... 28 2.5 Kinetic ............................................................................................................................... 29 2.6 Kinetic Parameters ........................................................................................................... 29 2.7 Using a Luminescence Light Plate ................................................................................. 29 2.7.1 Getting Started ...................................................................................................... 29 2.7.2 Running the Luminescence Light Plate Protocol .................................................. 30 2.7.3 Analyzing the Results from the Luminescence Light Plate ................................... 30 2.8 Luminescence Data .......................................................................................................... 31 2.8.1 Kinetic .................................................................................................................... 31 2.8.2 Ratio Readings ...................................................................................................... 31 2.9 Luminescence Module ..................................................................................................... 31 3 Fluorescence .............................................................................................................................. 32 3.1 General Information ......................................................................................................... 32 3.2 Optical Wavelengths and Commonly Used Fluorophores ........................................... 32 3.3 Fluorescence Data ........................................................................................................... 32 3.4 Switching Fluorescence Optical Kits ............................................................................. 33 3.5 Starting a Run .................................................................................................................. 33 3.6 Fluorescence Module ....................................................................................................... 34 3.6.1 Installing the Fluorescence Module ....................................................................... 34 4 Visible Absorbance .................................................................................................................... 35 4.1 General Information ......................................................................................................... 35 4.2 Optical Filter Wheels ........................................................................................................ 35 4.2.1 Customizable Absorbance Paddle ........................................................................ 35 4.3 Starting a Run ................................................................................................................... 36 4.4 Ratiometric Assay ............................................................................................................ 37 4.5 Absorbance Data .............................................................................................................. 37 4.5.1 Ratiometric Data .................................................................................................... 37 4.6 Absorbance Module ......................................................................................................... 38 4.6.1 Installing the Visible Absorbance Housing Unit .................................................... 38 4.6.1 Installing the Visible Absorbance Module ............................................................
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