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Use of Laboratory Equipement
USE OF LABORATORY EQUIPEMENT C. Laboratory Thermometers Most thermometers are based upon the principle that liquids expand when heated. Most common thermometers use mercury or colored alcohol as the liquid. These thermometers are constructed as that a uniform-diameter capillary tube surmounts a liquid reservoir. To calibrate a thermometer, one defines two reference points, normally the freezing point of water (0°C, 32°F) and the boiling point of water (100°C, 212°F) at 1 tam of pressure (1 tam = 760 mm Hg). Once these points are marked on the capillary, its length is then subdivided into uniform divisions called degrees. There are 100° between these two points on the Celsius (°C, or centigrade) scale and 180° between those two points on the Fahrenheit (°F) scale. °F = 1.8 °C + 32 The Thermometer and Its Calibration This section describes the proper technique for checking the accuracy of your thermometer. These measurements will show how measured temperatures (read from thermometer) compare with true temperatures (the boiling and freezing points of water). The freezing point of water is 0°C; the boiling point depends upon atmospheric pressure but at sea level it is 100°C. Option 1: Place approximately 50 mL of ice in a 250-mL beaker and cover the ice with distilled water. Allow about 15 min for the mixture to come to equilibrium and then measure and record the temperature of the mixture. Theoretically, this temperature is 0°C. Option 2: Set up a 250-mL beaker on a wire gauze and iron ring. Fill the beaker about half full with distilled water. -
Laboratory Glassware N Edition No
Laboratory Glassware n Edition No. 2 n Index Introduction 3 Ground joint glassware 13 Volumetric glassware 53 General laboratory glassware 65 Alphabetical index 76 Índice alfabético 77 Index Reference index 78 [email protected] Scharlau has been in the scientific glassware business for over 15 years Until now Scharlab S.L. had limited its sales to the Spanish market. However, now, coinciding with the inauguration of the new workshop next to our warehouse in Sentmenat, we are ready to export our scientific glassware to other countries. Standard and made to order Products for which there is regular demand are produced in larger Scharlau glassware quantities and then stocked for almost immediate supply. Other products are either manufactured directly from glass tubing or are constructed from a number of semi-finished products. Quality Even today, scientific glassblowing remains a highly skilled hand craft and the quality of glassware depends on the skill of each blower. Careful selection of the raw glass ensures that our final products are free from imperfections such as air lines, scratches and stones. You will be able to judge for yourself the workmanship of our glassware products. Safety All our glassware is annealed and made stress free to avoid breakage. Fax: +34 93 715 67 25 Scharlab The Lab Sourcing Group 3 www.scharlab.com Glassware Scharlau glassware is made from borosilicate glass that meets the specifications of the following standards: BS ISO 3585, DIN 12217 Type 3.3 Borosilicate glass ASTM E-438 Type 1 Class A Borosilicate glass US Pharmacopoeia Type 1 Borosilicate glass European Pharmacopoeia Type 1 Glass The typical chemical composition of our borosilicate glass is as follows: O Si 2 81% B2O3 13% Na2O 4% Al2O3 2% Glass is an inorganic substance that on cooling becomes rigid without crystallising and therefore it has no melting point as such. -
PML Brochure
PHYSICAL MEASUREMENT LABORATORY Gauging nature on all scales NIST.GOV/PML PHYSICAL MEASUREMENT LABORATORY (PML) The Physical Measurement Laboratory (PML), a major frequency, electricity, temperature, humidity, pressure operating unit of the National Institute of Standards and vacuum, liquid and gas flow, and electromag- and Technology (NIST), sets the definitive U.S. standards netic, optical, acoustic, and ionizing radiation. PML for nearly every kind of measurement in modern life, collaborates directly with industry, universities, profes- sometimes across more than 20 orders of magni- sional and standards-setting organizations, and other tude. PML is a world leader in the science of physical agencies of government to ensure accuracy and to measurement, devising procedures and tools that make solve problems. It also supports research in many continual progress possible. Exact measurements are fields of urgent national importance, such as manu- absolutely essential to industry, medicine, the research facturing, energy, health, law enforcement and community, and government. All of them depend on homeland security, communications, military defense, PML to develop, maintain, and disseminate the official electronics, the environment, lighting and display, standards for a wide range of quantities, including radiation, remote sensing, space exploration, length, mass, force and shock, acceleration, time and and transportation. IMPACTS ❱ Provides 700 kinds of calibration services ❱ Numerous special testing services NIST.GOV/PML | 2 NIST.GOV/PML -
Chemostat Culture for Yeast Experimental Evolution
Downloaded from http://cshprotocols.cshlp.org/ at Cold Spring Harbor Laboratory Library on August 9, 2017 - Published by Cold Spring Harbor Laboratory Press Protocol Chemostat Culture for Yeast Experimental Evolution Celia Payen and Maitreya J. Dunham1 Department of Genome Sciences, University of Washington, Seattle, Washington 98195 Experimental evolution is one approach used to address a broad range of questions related to evolution and adaptation to strong selection pressures. Experimental evolution of diverse microbial and viral systems has routinely been used to study new traits and behaviors and also to dissect mechanisms of rapid evolution. This protocol describes the practical aspects of experimental evolution with yeast grown in chemostats, including the setup of the experiment and sampling methods as well as best laboratory and record-keeping practices. MATERIALS It is essential that you consult the appropriate Material Safety Data Sheets and your institution’s Environmental Health and Safety Office for proper handling of equipment and hazardous material used in this protocol. Reagents Defined minimal medium appropriate for the experiment For examples, see Protocol: Assembly of a Mini-Chemostat Array (Miller et al. 2015). Ethanol (95%) Glycerol (20% and 50%; sterile) Yeast strain of interest Equipment Agar plates (appropriate for chosen strain) Chemostat array Assemble the apparatus as described in Miller et al. (2013) and Protocol: Assembly of a Mini-Chemostat Array (Miller et al. 2015). Cryo deep-freeze labels Cryogenic vials Culture tubes Cytometer (BD Accuri C6) Glass beads, 4 mm (sterile; for plating yeast cells) Glass cylinder Kimwipes 1Correspondence: [email protected] © 2017 Cold Spring Harbor Laboratory Press Cite this protocol as Cold Spring Harb Protoc; doi:10.1101/pdb.prot089011 559 Downloaded from http://cshprotocols.cshlp.org/ at Cold Spring Harbor Laboratory Library on August 9, 2017 - Published by Cold Spring Harbor Laboratory Press C. -
ELISA Plate Reader
applications guide to microplate systems applications guide to microplate systems GETTING THE MOST FROM YOUR MOLECULAR DEVICES MICROPLATE SYSTEMS SALES OFFICES United States Molecular Devices Corp. Tel. 800-635-5577 Fax 408-747-3601 United Kingdom Molecular Devices Ltd. Tel. +44-118-944-8000 Fax +44-118-944-8001 Germany Molecular Devices GMBH Tel. +49-89-9620-2340 Fax +49-89-9620-2345 Japan Nihon Molecular Devices Tel. +06-6399-8211 Fax +06-6399-8212 www.moleculardevices.com ©2002 Molecular Devices Corporation. Printed in U.S.A. #0120-1293A SpectraMax, SoftMax Pro, Vmax and Emax are registered trademarks and VersaMax, Lmax, CatchPoint and Stoplight Red are trademarks of Molecular Devices Corporation. All other trademarks are proprty of their respective companies. complete solutions for signal transduction assays AN EXAMPLE USING THE CATCHPOINT CYCLIC-AMP FLUORESCENT ASSAY KIT AND THE GEMINI XS MICROPLATE READER The Molecular Devices family of products typical applications for Molecular Devices microplate readers offers complete solutions for your signal transduction assays. Our integrated systems γ α β s include readers, washers, software and reagents. GDP αs AC absorbance fluorescence luminescence GTP PRINCIPLE OF CATCHPOINT CYCLIC-AMP ASSAY readers readers readers > Cell lysate is incubated with anti-cAMP assay type SpectraMax® SpectraMax® SpectraMax® VersaMax™ VMax® EMax® Gemini XS LMax™ ATP Plus384 190 340PC384 antibody and cAMP-HRP conjugate ELISA/IMMUNOASSAYS > nucleus Single addition step PROTEIN QUANTITATION cAMP > λEX 530 nm/λEM 590 nm, λCO 570 nm UV (280) Bradford, BCA, Lowry For more information on CatchPoint™ assay NanoOrange™, CBQCA kits, including the complete procedure for this NUCLEIC ACID QUANTITATION assay (MaxLine Application Note #46), visit UV (260) our web site at www.moleculardevices.com. -
HAAKE Viscometer Standard Operating Procedure [Updated Sept 10, 2014]
HAAKE Viscometer Standard Operating Procedure [Updated Sept 10, 2014] HAAKE Viscometer 7 R+ Location of Machine: Composites Lab, RFM 1218 Location of SOP and Machine Operating & Safety Manual: Composites Lab website under resources; Composites Lab TRACS site; and Hardcopy near machine. Emergency Contact: Call 911 Call EHS & Risk Management at 512-245-3616 Call Head Lab Technician, Dr. Ray Cook (office 512-245-2050) Call Dr. Jitendra S Tate (office 512-245-4872) Before using this machine: You must have permission from Dr. Tate. You must have received formal training from technician or, trained research student (designated by Dr. Tate) related to machine safety and operation. You must read and understand SOP and Machine Cleaning Manual. You must use this machine under direct supervision of Dr. Tate or, Dr. Cook or, trained research student (designated by Dr. Tate). You must have signed “Lab Rules” document with Dr. Tate. This document must be signed every semester fall, spring, and summer (as applicable). If you do NOT follow above instructions you will be held responsible for your own safety and damages. Safety Precautions: Protective Equipment: Prior to performing this procedure, the following personal protective equipment must be obtained and ready for use: Gloves, Safety Goggles, Face Mask, Lab Coat. Important Safeguards: 1. Prior to performing this procedure, the following safety equipment must be accessible and ready for use: (e.g. chemical fume hood, biological safety cabinet, laminar flow hood, chemical spill kits) Fume hood 2. All liquids should be drained to containers for chemical disposal and properly marked. 3. In the event that a hazardous material spill during this procedure, be prepared to clean with cleaner according to MSDS of materials used. -
Information Technology Laboratory Technical Accomplishments
CONTENTS Director’s Foreword 1 ITL at a Glance 4 ITL Research Blueprint 6 Accomplishments of our Research Program 7 Foundation Research Areas 8 Selected Cross-Cutting Themes 26 Industry and International Interactions 36 Publications 44 NISTIR 7169 Conferences 47 February 2005 Staff Recognition 50 U.S. DEPARTMENT OF COMMERCE Carlos M. Gutierrez, Secretary Technology Administration Phillip J. Bond Under Secretary of Commerce for Technology National Institute of Standards and Technology Hratch G. Semerjian, Jr., Acting Director About ITL For more information about ITL, contact: Information Technology Laboratory National Institute of Standards and Technology 100 Bureau Drive, Stop 8900 Gaithersburg, MD 20899-8900 Telephone: (301) 975-2900 Facsimile: (301) 840-1357 E-mail: [email protected] Website: http://www.itl.nist.gov INFORMATION TECHNOLOGY LABORATORY D IRECTOR’S F OREWORD n today’s complex technology-driven world, the Information Technology Laboratory (ITL) at the National Institute of Standards and Technology has the broad mission of supporting U.S. industry, government, and Iacademia with measurements and standards that enable new computational methods for scientific inquiry, assure IT innovations for maintaining global leadership, and re-engineer complex societal systems and processes through insertion of advanced information technology. Through its efforts, ITL seeks to enhance productivity and public safety, facilitate trade, and improve the Dr. Shashi Phoha, quality of life. ITL achieves these goals in areas of Director, Information national priority by drawing on its core capabilities in Technology Laboratory cyber security, software quality assurance, advanced networking, information access, mathematical and computational sciences, and statistical engineering. utilizing existing and emerging IT to meet national Information technology is the acknowledged engine for priorities that reflect the country’s broad based social, national and regional economic growth. -
The-Pathologists-Microscope.Pdf
The Pathologist’s Microscope The Pathologist’s Microscope Rudolf Virchow, the father of Pathology, had available to him wonderful microscopes during the 1850’s to 1880’s, but the one you have now is far better. Your microscope is the most highly perfected of all scientific instruments. These brief notes on alignment, the objective lens, the condenser, and the eyepieces are what you need to know to get the most out of your microscope and to feel comfortable using it. Figure 1 illustrates the important parts of a generic modern light microscope. Figure 1 - Parts of the Microscope UNC Pathology & Lab Med, MSL, July 2013 1 The Pathologist’s Microscope Alignment August Köhler, in 1870, invented the method for aligning the microscope’s optical system that is still used in all modern microscopes. To get the most from your microscope it should be Köhler aligned. Here is how: 1. Focus a specimen slide at 10X. 2. Open the field iris and the condenser iris. 3. Observe the specimen and close the field iris until its shadow appears on the specimen. 4. Use the condenser focus knob to bring the field iris into focus on the specimen. Try for as sharp an image of the iris as you can get. If you can’t focus the field iris, check the condenser for a flip-in lens and find the configuration that lets you see the field iris. You may also have to move the field iris into the field of view (step 5) if it is grossly misaligned. 5.Center the field iris with the condenser centering screws. -
Building and Validating a Rotational Viscometer Brian Cherrington & Jack Rothstein Mechanical Engineering Faculty Mentors: Dr
Building and Validating a Rotational Viscometer Brian Cherrington & Jack Rothstein Mechanical Engineering Faculty Mentors: Dr. Maria-Isabel Carnasciali, Dr. Samuel Daniels Abstract This project was an effort to redesign an initial prototype rotational viscometer to experimentally test whether or not viscosity values vary significantly when the geometry of the viscometer is changed. The scope of the project involved designing and building a viscometer that could vary the gap between the inner and outer cylinders, variation of the testing fluid’s temperature, and control of the device’s RPM. After weeks of planning, designing, and fabrication the new viscometer was complete. In order to control the device, monitor the sensor readings, and calculate the testing fluid’s viscosity a LabVIEW program was created. Testing on medium to high viscous fluids was completed to determine if the viscosity values and the geometry of the viscometer are dependent or independent of each other. The results did show a correlation between measured viscosity and variations in the geometry of the viscometer. More testing is required to further verify the results and properly calibrate the device. Introduction For this project a new design was conceptualized, fabricated, and tested. This new design met several criteria including, Viscosity is often referred to as a fluid’s thickness or how much it resists deformation due to an applied force. Designed for future use in ME labs; Rotational viscometers measure the amount of torque needed Designed to be durable, sustainable, and easy to to rotate an object moving through fluid at a known RPM. dissemble and clean; Using the measured torque, RPM, and dimensions of the Multiple inner cylinders for varying gap sizes; device, the viscosity can be calculated using equation 1. -
Miniav®-X Automatic Viscometer Instruction & Operation Manual
MiniAV®-X Automatic Viscometer Instruction & Operation Manual 81.2254 i CONTENTS 1 INTRODUCTION/INSTALLATION 1 The miniAV®-X Automatic Viscometer .................................................................................. 1 Measuring kinematic viscosity ............................................................................................... 2 Safety cautions ..................................................................................................................... 2 Specifications ....................................................................................................................... 4 Installation ............................................................................................................................ 4 Required installation components ............................................................................... 4 Vacuum Pump unit connections ................................................................................. 6 Bath unit connections ................................................................................................ 6 VISCPRO® for Windows® XP® ............................................................................................ 6 Installing VISCPRO® software .............................................................................................. 7 Computer requirements ............................................................................................. 7 Windows® XP® installation ....................................................................................... -
Microplate Selection and Recommended Practices in High-Throughput Screening and Quantitative Biology
NLM Citation: Auld DSPh.D., Coassin PAB.S., Coussens NPPh.D., et al. Microplate Selection and Recommended Practices in High-throughput Screening and Quantitative Biology. 2020 Jun 1. In: Sittampalam GS, Grossman A, Brimacombe K, et al., editors. Assay Guidance Manual [Internet]. Bethesda (MD): Eli Lilly & Company and the National Center for Advancing Translational Sciences; 2004-. Bookshelf URL: https://www.ncbi.nlm.nih.gov/books/ Microplate Selection and Recommended Practices in High-throughput Screening and Quantitative Biology Douglas S. Auld, Ph.D.,1 Peter A. Coassin, B.S.,2 Nathan P. Coussens, Ph.D.,3 Paul Hensley,4 Carleen Klumpp-Thomas,5 Sam Michael,5 G. Sitta Sittampalam, Ph.D.,5 O. Joseph Trask, B.S.,6 Bridget K. Wagner, Ph.D.,7 Jeffrey R. Weidner, Ph.D.,8 Mary Jo Wildey, Ph.D.,9 and Jayme L. Dahlin, M.D., Ph.D. 7,10 Created: June 1, 2020. Abstract High-throughput screening (HTS) can efficiently assay multiple discrete biological reactions using multi-well microplates. The choice of microplate is a crucial yet often overlooked technical decision in HTS and quantitative biology. This chapter reviews key criteria for microplate selection, including: well number, well volume and shape, microplate color, surface treatments/coatings, and considerations for specialized applications such as high-content screening. This chapter then provides important technical advice for microplate handling including mixing, dispensing, incubation, and centrifugation. Special topics are discussed, including microplate surface properties, plate washing, well-to-well contamination, microplate positional effects, well-to-well and inter-lot variability, and troubleshooting. This information and best practices derived from academic, government, and industry screening centers, as well as microplate vendors, should accelerate assay development and enhance assay quality. -
List of Equipments in the Department (Chemical Engineering) Mechanical
List of Equipments in the Department (Chemical Engineering) Mechanical Operation lab Fluid Mechanics Lab Heat transfer Lab Mass Transfer lab • Cyclone Separator • Reynold’s Apparatus • Double Pipe Heat Exchanger • Tray Dryer • Plate & Frame Filter Press • Bernoulli’s Theorem Apparatus • Shell & Tube Heat Exchanger • Sieve Plate Distillation Column • Vibrating Screen • Pitot Tube Apparatus • Vertical Condenser • Liquid-Liquid Extraction • Jaw Crusher • Calibration of Orifice meter, • Computerized Control Shell & • Adsorption of CO 2 • Ball Mill Venturi meter and Rota meter Tube Heat Exchanger • Steam Distillation • Roll Crusher • Coefficient of Discharge of • Thermal Conductivity of Metal • Bubble Cap Distillation Column • Rotary Vacuum filter Orifice and Mouthpiece Bar • Cooling Tower • Fluidized Bed • Film & Drop Wise Condensation • Diffusivity Apparatus • Single Effect Evaporator • Fluidized Bed Dryer • Simple Steam Distillation • Simple Distillation • VLE Apparatus • Equilibrium Flash Distillation • Humidification & Dehumidification • Refractometer Process Control Lab Chemical Reaction Engg. Lab Fuel Combustion Energy Technology Lab Environmental Lab • Process Training Simulator with • Plug Flow Reactor • Flash & Fire Point Apparatus • BOD Incubator Modules (Software) • Isothermal Batch Reactor • Aniline Point Apparatus • pH Meter • Pressure Control System • Single Tube Packed Bed Reactor • Orsat Gas Analysis Apparatus • Sedimentation Apparatus • Flow Control System • RTD in CSTR / Mixed flow • Redwood Viscometer • UV-VIS Spectrophotometer • pH control System Reactor • Bomb Calorimeter • DO Meter • Real Time Simulator Trainer • RTD in Tubular Reactor • Smoke Point Apparatus • Digital Conductivity Meter • Level Control System • Adiabatic Batch Reactor • Temperature Control System • Distillation Column • Two Tank Interacting system • Two Tank Non-Interacting System • CSTR Control System Research Equipments • UV-Spectrophotometer • Gas Chomatography .