Improvements to the Maize (Zea Mays L.) in Vivo Maternal Doubled Haploid System Gerald Neil De La Fuente Iowa State University
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Iowa State University Capstones, Theses and Graduate Theses and Dissertations Dissertations 2015 Improvements to the maize (Zea mays L.) in vivo maternal doubled haploid system Gerald Neil De La Fuente Iowa State University Follow this and additional works at: https://lib.dr.iastate.edu/etd Part of the Agriculture Commons, Genetics Commons, and the Plant Sciences Commons Recommended Citation De La Fuente, Gerald Neil, "Improvements to the maize (Zea mays L.) in vivo maternal doubled haploid system" (2015). Graduate Theses and Dissertations. 14767. https://lib.dr.iastate.edu/etd/14767 This Dissertation is brought to you for free and open access by the Iowa State University Capstones, Theses and Dissertations at Iowa State University Digital Repository. It has been accepted for inclusion in Graduate Theses and Dissertations by an authorized administrator of Iowa State University Digital Repository. For more information, please contact [email protected]. Improvements to the maize (Zea mays L.) in vivo maternal doubled haploid system by Gerald N. De La Fuente A dissertation submitted to the graduate faculty in partial fulfillment of the requirements for the degree of DOCTOR OF PHILOSOPHY Major: Plant Breeding Program of Study Committee: Thomas Lübberstedt, Major Professor Dan Nettleton Michael Blanco Paul Scott Erik Vollbrecht Iowa State University Ames, Iowa 2015 Copyright © Gerald N. De La Fuente, 2015. All rights reserved. ii DEDICATION This dissertation is dedicated to Mr. Mark J. Lamon. My passion for agriculture was found working on the Lamon family farm, and it continues to this day. Many were the hours spent discussing farming and agriculture with Mr. Lamon, and the lessons I learned were put to use in the development of this dissertation. Thank you, Mark. iii TABLE OF CONTENTS DEDICATION ......................................................................................................... ii ABSTRACT ......................................................................................................... vi CHAPTER ONE: GENERAL INTRODUCTION .................................................... 1 Evolution of maize breeding ................................................................................ 2 Doubled haploids ................................................................................................. 3 Organization of the thesis .................................................................................... 9 References ......................................................................................................... 11 CHAPTER TWO: A DIALLEL ANALYSIS OF A MAIZE DONOR POPULATION RESPONSE TO IN VIVO MATERNAL HAPLOID INDUCTION I: INDUCIBILITY .............................................................................. 15 Abstract ......................................................................................................... 15 Introduction ......................................................................................................... 16 Materials and methods ......................................................................................... 20 Results ......................................................................................................... 24 Discussion ......................................................................................................... 26 References ......................................................................................................... 31 Tables and figures ................................................................................................ 34 CHAPTER THREE: REGISTRATION OF BHI306 MAIZE MATERNAL HAPLOID INDUCER GERMPLSAM ..................................................................... 38 Abstract ......................................................................................................... 38 Introduction ......................................................................................................... 38 Methods ......................................................................................................... 43 Characteristics ...................................................................................................... 45 iv Availability ......................................................................................................... 47 References ......................................................................................................... 48 Tables and figures ................................................................................................ 50 CHAPTER FOUR: DISCRIMINATION OF HAPLOID AND DIPLOID MAIZE KERNELS VIA MULTISPECTRAL IMAGING ..................................................... 52 Abstract ......................................................................................................... 52 Introduction ......................................................................................................... 53 Methods ......................................................................................................... 56 Results ......................................................................................................... 62 Discussion ......................................................................................................... 64 References ......................................................................................................... 70 Tables and figures ................................................................................................ 73 CHAPTER FIVE: A DIALLEL ANALYSIS OF A MAIZE DONOR POPULATION RESPONSE TO IN VIVO MATERNAL HAPLOID INDUCTION II: SPONTANEOUS CHROMOSOME DOUBLING .............................................. 84 Abstract ......................................................................................................... 84 Introduction ......................................................................................................... 85 Materials and methods ......................................................................................... 88 Results ......................................................................................................... 92 Discussion ......................................................................................................... 94 References ......................................................................................................... 103 Tables and figures ................................................................................................ 107 CHAPTER SIX: ACCELERATING PLANT BREEDING ...................................... 114 Abstract ......................................................................................................... 114 Glossary box ........................................................................................................ 114 v Keeping up with demand ..................................................................................... 116 The breeder’s equation ......................................................................................... 117 Speeding up ......................................................................................................... 118 The in vitro nursery .............................................................................................. 118 Challenges ......................................................................................................... 123 References ......................................................................................................... 126 Boxes ......................................................................................................... 134 Figures ......................................................................................................... 136 CHAPTER SEVEN: GENERAL CONCLUSIONS AND THE FUTURE OF MATERNAL IN VIVO DOUBLED HAPLOIDS ..................................................... 141 ACKNOWLEDGEMENTS ....................................................................................... 146 vi ABSTRACT The maize (Zea mays L.) in vivo maternal doubled haploid system has been widely applied to maize breeding and genetics in recent decades and is an important part of the majority of public and private maize breeding programs today. The principal advantage of the doubled haploid system is the ability to generate completely homozygous inbred lines in as little as two seasons. Other advantages to this system include more rapid integration of loci of interest and increased usefulness over traditional lines developed through self-pollination. In this dissertation, some of the major problems in the maternal doubled haploid system are addressed. Namely, improvement of maternal inducers, improved understanding of the genetics controlling inducibility, development of an automated system to sort haploid kernels, investigation and application of spontaneous chromosome doubling, and a proposal for the acceleration of the breeding cycle beyond doubled haploids through the in vitro nursery. This dissertation provides some new insight into these problems, as follows. The development and release of a new improved maternal haploid inducer for use in doubled haploid programs. Improved understanding of the quantitative nature of inducibility and the effects of misclassification are discussed. Successful automated discrimination of haploid and diploid kernels using optical and fluorescence methods is described. In