2nd International Conference and Determination of antioxidant activity and phenolic profile of Paliurus spina-christi P. Mill. Exhibition on Pharmacology and Ethnopharmacology Sevim Küçük1, Fatih Göger2, Emre Kayalar1 May 02-04, 2016 Chicago, Illinois, USA 1 Anadolu University, Faculty of Pharmacy, Department of Pharmaceutical Botany, Eskisehir, Turkey 2 Anadolu University, Faculty of Pharmacy, Department of Pharmacognosy, Eskisehir, Turkey

Paliurus spina-christi P. Mill. (Rhamnaceae), also known as “Christ's thorn, Karaçalı or Çaltı”, is a

perennial thorny shrub of widespread distribution in dry and rocky places in the Mediterranean -EPI (300.73) Charge (+0) CE (20) FT (250): Exp 3, 31.353 min from Sample 1 (FG135V (SA 1023) Paliurus spina cristi) of FG135V (SA 1023) Pali... Max. 2.2e5 cps. 300.7 2.2e5 2.1e5

2.0e5 region and Asia, and the unique species of the genus Paliurus represented in Anatolia. Christ's 1.9e5 1.8e5

1.7e5 thorn is used as a diuretic, against diarrhoea and rheumatism in traditional Turkish herbal 1.6e5 1.5e5

1.4e5

1.3e5 medicine. The aim of this study was to determine the antioxidant activity and phenolic profile of 1.2e5 1.1e5

1.0e5

Intensity, cps Paliurus spina-christi. 9.0e4 8.0e4

7.0e4

6.0e4 5.0e4 178.8 4.0e4 150.8

3.0e4 For this purpose plant materials were collected from Eskişehir:Bozdağ in Turkey on August 2015. 2.0e4 1.0e4 272.8 0.0 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 1000 Powdered dried fruits were macerated with methanol at 25 °C for 24h. After evaporation and m/z, Da lyofilization steps the extract was analyzed with ABsciex 3200 Q trap LC-MS/MS system. Quercetin Paliurus spina-christi P. Mill.

-EPI (576.61) Charge (+1) CE (20) FT (250): Exp 3, 11.058 min from Sample 1 (FG135V (SA 1023) Paliurus spina cristi) of FG135V (SA 1023) Pali... Max. 6.7e5 cps.

576.4 6.7e5 Catechin hydrate, proanthocyanidin B dimer, naringenin-C-diglycoside, 5-caffeoylquinic acid 5-p- 6.5e5

6.0e5

coumaroylquinic acid, rutin, quercetin hexoside and quercetin were found as major compounds. 5.5e5

5.0e5 Extract presented strong antioxidant activity with the score of DPPH IC50=0.062 mg/ml. IC 50 of 4.5e5 4.0e5

406.8 3.5e5 BHT was determined 0,054 mg/ml. Total phenolic content of the extract was found as same 109.54 424.7 3.0e5

Intensity, cps

2.5e5

mgGAE in 1gr extract. 2.0e5

1.5e5 288.9 1.0e5 450.8 124.9 5.0e4 286.8 299.3 0.0 This study was supported by the Anadolu University, Commission of the Scientific Research Projects. 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 1000 m/z, Da

B type proanthocyanidin Paliurus spina-christi P. Mill.

-EPI (288.83) Charge (+1) CE (20) FT (250): Exp 3, 13.488 min from Sample 1 (FG135V (SA 1023) Paliurus spina cristi) of FG135V (SA 1023) Pali... Max. 5.0e5 cps.

288.8 5.0e5 4.8e5 4.6e5 4.4e5 4.2e5 4.0e5 3.8e5

3.6e5 244.9 3.4e5 3.2e5 3.0e5 2.8e5 2.6e5 2.4e5

Intensity,2.2e5 cps 2.0e5 1.8e5 1.6e5 Paliurus spina-christi P. Mill. LC-MS/MS system 1.4e5 1.2e5 205.1 1.0e5 179.0 8.0e4 6.0e4 137.0 161.2 4.0e4 123.1 221.0246.8 2.0e4 167.0 270.8 147.0 189.0 0.0 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 1000 m/z, Da

-EPI (352.73) Charge (+0) CE (30) FT (250): Exp 4, 15.771 min from Sample 1 (FG135V (SA 1023) Paliurus spina cristi) of FG135V (SA 1023) Pali... Max. 1.2e5 cps. -EPI (609.36) Charge (+1) CE (30) FT (250): Exp 4, 24.689 min from Sample 1 (FG135V (SA 1023) Paliurus spina cristi) of FG135V (SA 1023) Pali... Max. 2.1e5 cps.

190.8 608.5 2.1e5 Paliurus spina-christi P. Mill. 1.20e5 2.0e5 1.15e5 1.9e5 Catechin 1.10e5 1.8e5 299.9 1.05e5 1.7e5 1.00e5 9.50e4 1.6e5

9.00e4 1.5e5 8.50e4 1.4e5 8.00e4 1.3e5 7.50e4 1.2e5 7.00e4 6.50e4 1.1e5 6.00e4 1.0e5

Intensity,5.50e4 cps Intensity,9.0e4 cps 5.00e4 8.0e4 Baytop, A. 1977, Farmasötik Botanik, İÜ Eczacılık Fakültesi Yayın No:25, İstanbul, 407. 4.50e4 7.0e4 4.00e4 3.50e4 6.0e4 Guner ,A.; Aslan, S.; Ekim, T.; Vural, M.; Babac, M.-T. (ed).; A checklist of the Flora of Turkey (vascular plants), Nezahat Gökyiğit Botanik 3.00e4 5.0e4

2.50e4 4.0e4 2.00e4 3.0e4 1.50e4 Bahçesi Yayınları, Flora Dizisi 1, 12-14, Kasım 2012. 2.0e4 270.8 1.00e4 178.9 254.8 150.8 5000.00 1.0e4 302.6 342.8 610.4 0.00 0.0 Tanker N., Koyuncu M., Coşkun, M. 2014. Farmasötik Botanik, Ankara Üniversitesi, Eczacılık Fakültesi Yayın No:105, Ankara, 258. 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 1000 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 1000 m/z, Da m/z, Da 5-Caffeoylquinic acid (30 Ev) Rutin