Rapid Communication- Primer Design and Sequence Information for Cytochrome B Gene in the Chinese Painted Quail (Excalfactoria C
-Rapid Communication- Primer Design and Sequence Information for Cytochrome b Gene in the Chinese Painted Quail (Excalfactoria chinensis) Xiang-Jun SHEN, Masaoki TSUDZUKIa, Takao NAKAMURAb The United Graduate School of Agricultural Science, Gifu University, Gifu-shi 501-1193, Japan Faculty of Applied Biological Science, Hiroshima University, Higashi-Hiroshima-shi 739-8528,a Japan bFaculty of Agriculture , Gifu University, Gifu-shi 501-1193, Japan (Received April 21, 1999; Accepted May 15, 1999) Abstract In accordance with the chicken and quail mitochondrialDNA sequences,three pairs of primers encompassing light and heavy strand (L14832, L15269, L15500, H15924, H15497, and H16207) with high specificity were designed, synthesized, and used for amplification and sequencing of cytochrome b (Cyt b) gene. The PCR products were sequenced by the direct-sequencing method. The Chinese painted quail cytochrome b gene consisted of 1,143bp nucleotides (base composition: 327 A; 399 C; 281 T and 136 G), encoded for a 380 amino acid sequence, and showed 89.0%, 86.4%, and 86.2%, homology with nucleotide sequences of Japanese quail, silky fowl, and White Leghorn chicken, respectively. The homology of the amino acid sequence of the cytochrome b gene of Chinese painted quail showed 98.2%, 95.3%, and 95.3% with those of Japanese quail, silky fowl, and White Leghorn chicken, respectively. The sequencesreported in this paper have been depositedin the GenBank database(Accession No. AF109217). Animal Science Journal 70 (4): 240-242,1999 Key words: Chinese painted quail,Excalfactoria chinensis, Cytochromeb, Sequence,Homology Chinese painted quail (Excalfactoria chinensis) belongs the test: to the order Galliformes, family Phasianidae, genus (1) L14832: 5'-TACCTGGGTTCCTTCGCCCT-3' Excalfactoria5),and it is the smallest species in the family (2) H15497: 5'-TGTAGGAAGGTGAGTGGAT-3' Phasianidae.
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